CN109320345A - It is a kind of for improving the complex micro organism fungicide and purposes of crop yield and quality - Google Patents

It is a kind of for improving the complex micro organism fungicide and purposes of crop yield and quality Download PDF

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Publication number
CN109320345A
CN109320345A CN201811264239.4A CN201811264239A CN109320345A CN 109320345 A CN109320345 A CN 109320345A CN 201811264239 A CN201811264239 A CN 201811264239A CN 109320345 A CN109320345 A CN 109320345A
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micro organism
complex micro
organism fungicide
kinds
quality
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CN201811264239.4A
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秦秦
薛永
宋科
孙丽娟
孙雅菲
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Shanghai Academy of Agricultural Sciences
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Shanghai Academy of Agricultural Sciences
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D5/00Fertilisers containing magnesium
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention discloses a kind of complex micro organism fungicide and its in the application for improving crop yield and quality, it is related to microorganism field.The present invention provides a kind of complex micro organism fungicides, are mixed by composite bacteria agent, microelement, diatomite and desiccant;The composite bacteria agent is made of bacillus subtilis, bacillus licheniformis, bacillusmusilaginosiengineering and lactobacillus plantarum.The various probiotics reasonable compatibilities for forming dominant microflora can be applied in agricultural land soil by complex micro organism fungicide of the invention by a certain percentage, be on the one hand conducive to improvement of soil fertility, reduced soil-borne disease, improve chemical fertilizer utilization ratio;On the other hand crop root vigor and resistance are improved, plant is inhibited to absorb heavy metal-polluted soil and pesticide residue, improves crop yield and quality.

Description

It is a kind of for improving the complex micro organism fungicide and purposes of crop yield and quality
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of complex micro organism fungicide answering in agricultural production With.
Background technique
In recent years, with the fast development of China's agricultural economy, soil-borne disease aggravation, soil are generally faced in agricultural production The problems such as microbial population is unbalance, soil fertility declines, heavy metal pollution and pesticide residue are serious, chemical fertilizer utilization ratio is low, into And lead to crop yield and quality decline.
Soil Microorganism is many kinds of, including bacterium, fungi and actinomyces etc., these microorganisms are all soil life entities Important component, it is directly or indirectly closely related with plant growth.Beneficial microbe can be fixed in plant rhizosphere in soil Grow, the relationship of mutualistic symbiosis established with plant: on the one hand can by phosphorus decomposing, potassium decomposing and nitrogen fixation improve the soil nitrogen, phosphorus and The bio-available Zn concentration of potassium nutrition element;On the other hand a large amount of auxins, stimulation and regulating crop growth can be also discharged, is promoted Into the absorption to nutrient.However natural microorganisms activity is low, probiotics are hardly formed dominant microflora.This patent is according to plant Interaction relationship between object and microorganism, using it is two or more and mutually not made of the beneficial microbe strain of antagonism it is compound micro- Bacteria agent is nontoxic and include a large amount of beneficial to viable bacteria substance and natural fermented active material and efficient republicanism yield increasing fungus, energy It is enough quickly to establish beneficial microbe dominant microflora, inhibit pernicious bacteria, activates root system of plant, stimulation and regulating crop growth, promote Soil mineral is enriched in absorption into crop to nutrient, improves stress resistance of plant, while inhibiting plant to heavy metal and agriculture Medicine residual absorbant, to increase agricultural product quality and safety.
Summary of the invention
It is an object of that present invention to provide a kind of complex micro organism fungicides can apply to agricultural production, the microbial composite bacteria Agent has activation root system of plant, promotes plant growth, improves the function of crop yield and quality.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
It is a kind of for improving the complex micro organism fungicide of crop yield and quality, it is by composite bacteria agent, microelement, silicon What diatomaceous earth and desiccant mixed;The composite bacteria agent is by bacillus subtilis, bacillus licheniformis, gel-shaped gemma bar Bacterium and lactobacillus plantarum composition.
Complex micro organism fungicide of the invention can form 4 kinds the probiotics reasonable compatibility of dominant microflora, by certain ratio Example is applied in agricultural land soil, is on the one hand conducive to improvement of soil fertility, is reduced soil-borne disease, improves chemical fertilizer utilization ratio;On the other hand It improves crop root vigor and resistance, plant is inhibited to absorb heavy metal-polluted soil and pesticide residue, improve crop yield and product Matter.
Preferably, bacillus subtilis, bacillus licheniformis, bacillusmusilaginosiengineering and lactobacillus plantarum mass ratio For 2:1:1:1.
Preferably, during bacillus subtilis, bacillus licheniformis, bacillusmusilaginosiengineering and lactobacillus plantarum be purchased from Agricultural Microbiological Culture Collection administrative center of state, deposit number be respectively ACCC01185, ACCC01655, ACCC01434 and ACCC10171。
Preferably, the microelement is made of zinc sulfate, magnesium sulfate and borax.Further, the microelement In, the mass ratio of zinc sulfate, magnesium sulfate and borax is 1:2:1.
Preferably, the desiccant is one of bentonite, silica gel, white carbon black or attapulgite.
Another object of the present invention is to provide a kind of for improving the complex micro organism fungicide of crop yield and quality Preparation method, its step are as follows:
It 1) is 20~35 DEG C in temperature, under confined conditions, by bacillus subtilis, bacillus licheniformis, gel-shaped gemma 4 kinds of microorganism fungus kinds of bacillus and lactobacillus plantarum are inoculated with respectively on activation medium, will after activation culture 24~48 hours Cultured strain respectively expand numerous 24~72 hours in production fermentation medium by fermentation, expands numerous process in activation culture and fermentation In be stirred continuously, revolving speed 180rpm obtains the bacterium solution of above-mentioned 4 kinds of microorganism fungus kinds;
2) it is pre-processed the bacterium solution of the resulting 4 kinds of microorganism fungus kinds of step 1) to obtain the bacterium powder of various strains respectively, The pretreatment is adsorbed for first 1:5 ratio in mass ratio with diatomite support, then again 35~40 DEG C of low temperature dryings to water Divide less than 15%, being finally crushed to granularity is 40 mesh;After the bacterium powder of 4 kinds of microorganism fungus kinds is mixed, with the desiccant of crushing, micro- Secondary element uniformly mixes, and obtains complex micro organism fungicide.
Preferably, the activation medium by beef extract 5g, peptone 5g, sodium chloride 5g and 1000 mL of water prepare and At pH 6.5, the strain 10% of activation is inoculated in production fermentation culture;The preparation method of the production fermentation culture Are as follows: by starch 10kg, beancake powder 10kg, peptone 1kg, ammonium sulfate 1kg, potassium dihydrogen phosphate 0.5kg, yeast powder 0.1kg and carbon After sour calcium 5kg mixing, it is settled to 1t with water, adjusts pH 7.5;Aerobic fermentation ventilatory capacity is 0.6~0.8V/Vmin.
Preferably, the activation medium and production fermentation medium respectively at 121 DEG C preparatory high-temperature sterilization 30~ After 50min, adds various microorganism fungus kinds and cultivated.
Another object of the present invention is to provide a kind of above-mentioned complex micro organism fungicides to improve crop yield and quality On purposes.
Preferably, the crops be industrial crops, including greengrocery, melon and fruit class, fruit tree kind, tealeaves class, herbs Class, Flowers class crop.
Further, the crops are strawberry.
The beneficial effects of the present invention are: the present invention provides a kind of complex micro organism fungicide that can be applied to agricultural production, In order to further analyze influence of the complex micro organism fungicide to crop growth, complex micro organism fungicide is carried out to strawberry Manure trial.The result shows that application complex micro organism fungicide can promote strawberry nutrition growth and leaf growth development, changing Clear superiority is showed in terms of the kind economic characters such as strawberry single plant fruit number and single fruit weight, and makes the obvious effect of increasing production of strawberry.It is multiple The use for closing microbial bacterial agent can increase chemical fertilizer utilization ratio, greatly reduce the usage amount of chemical fertilizer, alleviate to a certain extent Pollution of area source;And by microorganism conversion, it is able to suppress harmful microorganism flora, activates plant root, absorption and passivation weight Metal and pesticide residue have remarkable result to crop yield and quality is improved.
Specific embodiment
The present invention is further elaborated and is illustrated With reference to embodiment.Each embodiment in the present invention Technical characteristic under the premise of not conflicting with each other, can carry out the corresponding combination.
Following implementation examples facilitate a better understanding of the present invention, but do not limit the present invention.Experiment in following embodiments Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even Mean value.
Embodiment 1: complex micro organism fungicide preparation
Complex microbial inoculum used in the present embodiment is purchased from Chinese agriculture Microbiological Culture Collection administrative center (Agricultural Culture Collection of China, ACCC), corresponding strain generic name and deposit number are as follows Table:
Chinese Deposit number Generic name
Bacillus subtilis ACCC01185 Bacillus subtilis
Bacillus licheniformis ACCC01655 Bacillus licheniformis
Gel-shaped bacillus ACCC01434 Bacillus mucilaginosus
Lactobacillus plantarum ACCC10171 Lactobacillus plantarum
Activation medium used in the present embodiment is prepared by beef extract 5g, peptone 5g, sodium chloride 5g, 1000 mL of water, PH is 6.5.
Used in the present embodiment production fermentation culture the preparation method comprises the following steps: by starch 10kg, 10 kg of beancake powder, egg White peptone 1kg, ammonium sulfate 1kg, potassium dihydrogen phosphate 0.5kg, yeast powder 0.1kg and calcium carbonate 5kg are uniformly mixed, and use is water-soluble 1t is solved and be settled to, pH 7.5 is adjusted.
Above-mentioned activation medium and production fermentation medium before use, be required at 121 DEG C high-temperature sterilization 30~ After 50min, the strain for adding various bacillus and lactobacillus plantarum is cultivated.
Complex micro organism fungicide is prepared via a method which:
(1) culture of various microbial inoculums: it is 25 DEG C in temperature, under confined conditions, above-mentioned 4 kinds of microorganism fungus kinds is existed It is inoculated with simultaneously after activation culture 48 hours respectively on above-mentioned activation medium, takes 10% to ferment in production respectively cultured strain Fermentation is expanded numerous 36 hours in culture medium, is stirred continuously during above-mentioned activation culture and fermentation expansion are numerous, revolving speed 180rpm has Aerobe fermentation ventilatory capacity is 0.7 (V/Vmin), obtains above-mentioned 4 kinds of bacillus subtilises, bacillus licheniformis, gel-shaped gemma bar The bacterium solution of bacterium and lactobacillus plantarum;
(2) preparation of complex micro organism fungicide: by the resulting bacillus subtilis of step (1), bacillus licheniformis, jelly The bacterium solution of sample bacillus and lactobacillus plantarum is pre-processed to obtain the bacterium powder of various strains, pretreated specific practice respectively Are as follows: first 1:5 ratio in mass ratio adsorbed with diatomite support, then again 35 DEG C of low temperature dryings to moisture less than 15%, most After be crushed to granularity be 40 mesh.By above-mentioned resulting bacillus subtilis, bacillus licheniformis, bacillusmusilaginosiengineering and plant The bacterium powder of lactobacillus obtains composite bacteria agent, desiccant, the micro member of composite bacteria agent and crushing after mixing according to mass ratio 1:2:1 Plain uniformly mixing, obtains complex micro organism fungicide.Each component content calculates as follows in mass ratio in complex micro organism fungicide: compound Microbial inoculum 40%, microelement 5%, diatomite 50%, desiccant 5%.Desiccant is bentonite.
This microbial inoculum uses three kinds of bacillus body (bacillus subtilis Bacillus based on bacillus subtilis Subtilis, bacillus licheniformis Bacillus licheniformis, bacillusmusilaginosiengineering Bacillus Mucilaginosus) co-conditioning soil, mass propagation is grown in the soil, establishes advantage profitable strain, inhibits pathogenic bacteria raw It is long, and fixed nitrogen and the microelements such as soluble phosphorus potassium element and Calcium Magnesium Sulphur, iron, violent, molybdenum can be released, promote soil fertility, The absorbable nutrient utilized, while a kind of compound lactobacillus plantarum with rhizosphere growth-promoting function are provided for crop, is improved Crop rhizosphere vigor and disease resistance promote the nutrient absorption and growth metabolism of crop, improve crop yield and quality.Furthermore it is multiple The lactobacillus plantarum of conjunction is that viable count is higher with the difference of other lactobacillus, and the big volume production acid of energy, stable soil pH value, with The acidic materials of three kinds of bacillus outputs inhibit crop also to have centainly the absorption of heavy metal to Heavy Metals in Soils is reduced Effect, can be improved crop product quality and foodsafety.
This microbial inoculum is combined proportion, bacillus subtilis, lichens gemma bar by composite bacillus and lactobacillus plantarum The mass ratio of bacterium, bacillusmusilaginosiengineering and lactobacillus plantarum is 2:1:1:1, gemma bar of the microbial inoculum headed by bacillus subtilis Based on bacterium, soil mineral can be enriched, release the nutrient and microelement easily absorbed, increase soil fertility.
Embodiment 2: application test of the complex micro organism fungicide on strawberry
The complex micro organism fungicide being prepared in the present embodiment Example 1 is tested.This test is in Shanghai City Min It is carried out in the village Du Hang of row area Pujiang town.Experimental plot topography is flat, and soil fertility is uniform, soil types rice soil, earth in quality.Before test Pedotheque is acquired for measuring analysis soil nutrient basic condition (being shown in Table 1).Experimental plot planting plants are strawberry (kind: red Face).Specific experimental design is as follows:
Test is that facility plastic greenhouse is cultivated, and planting density is 6500 plants/acre, and the uniform plot of fertility is selected to do plot experiment, A cell is arranged in vertical direction along plot P balance direction, and test sets 3 processing, 3 repetitions of each processing, random area Group arrangement, each each 30 ㎡ of plot area.This test is different except process content, and each field agronomic measures that handle (such as pour water, is miscellaneous Careless prevention and control of plant diseases, pest control etc.) it is consistent.
Processing 1: conventional fertilizer application+microbial bacterial agent (prepares) 50kg/ mus in embodiment 1, applies with base manure.
Processing 2: conventional fertilizer application+inactivation matrix (the inactivation microbial bacterial agent with 1 equivalent of processing).
Processing 3: conventional fertilizer application.
Its biological character is investigated in growth period, calculates per mu yield in harvest.
1) complex micro organism fungicide is to strawberry biological character and at the influence of the factor of production
Application complex micro organism fungicide (processing 1) can promote strawberry nutrition to grow, and promote leaf growth development, facilitate At the formation (as shown in table 1) of the factor of production.Specifically, processing 1 is in plant height, leaf blade size, vane thickness, single plant fruit number and single fruit Double recipe face shows more apparent advantage.Compared with processing 2, processing 3, handles 1 plant height and increase separately 0.9cm,1.3cm;Leaf blade size has increased separately 1.7cm2、 2.6cm2, single plant fruit number increases 0.1,0.4, single fruit weight Increase 1.6g, 2.2g;Processing 2 increases 0.4cm than 3 plant heights of processing, and leaf blade size increases 0.9cm2, single plant fruit number increases 0.3, single fruit weight increases 0.6g.
Respectively influence of the processing to strawberry biological character of table 1
2) influence result of the complex micro organism fungicide to yield of strawberry
Applying complex micro organism fungicide (processing 1) has significant effect (as shown in table 2) to strawberry volume increase.Specifically, Handling 1 cell average product to convert into per mu yield is 1786.8kg/ mus, than handling 2 102.2kg/ mus high, rate of growths 6.06%;Processing 1 It is 171.1kg/ mus higher than 3 per mu yields of processing, rate of growth 10.58%;It is 68.9kg/ mus higher than processing 3 to handle 2 per mu yields, rate of growth is 4.26%.Influence result by each processing to yield carries out variance analysis and Multiple range test (table 4, table 5), processing using LSD method 1, the yield between processing 2 reaches 5% level of signifiance, and the yield between processing 1 and processing 3 reaches 1% extremely significant level;Processing Difference is not significant between 2 and processing 3.
Respectively influence of the processing to yield of strawberry of table 2
3 yield of strawberry analysis of variance table of table
Respectively processing yield of strawberry comparison in difference table (LSD method) of table 4
It is indicated above that application complex micro organism fungicide can promote strawberry nutrition growth and leaf growth development, improving Clear superiority is showed in terms of the economic characters such as strawberry single plant fruit number and single fruit weight, and makes the obvious effect of increasing production of strawberry.
Above-mentioned embodiment is only a preferred solution of the present invention, so it is not intended to limiting the invention.Have The those of ordinary skill for closing technical field can also make various changes without departing from the spirit and scope of the present invention Change and modification.For example, above-mentioned complex micro organism fungicide can be used for the increasing yield and improving quality of other crops, might not be limited to Strawberry.Therefore all mode technical solutions obtained for taking equivalent substitution or equivalent transformation, all fall within protection model of the invention In enclosing.

Claims (10)

1. a kind of for improving the complex micro organism fungicide of crop yield and quality, which is characterized in that by composite bacteria agent, micro What element, diatomite and desiccant mixed;The composite bacteria agent is by bacillus subtilis, bacillus licheniformis, jelly Sample bacillus and lactobacillus plantarum composition.
2. complex micro organism fungicide as described in claim 1, which is characterized in that bacillus subtilis, bacillus licheniformis, glue The mass ratio for freezing sample bacillus and lactobacillus plantarum is 2:1:1:1.
3. complex micro organism fungicide as described in claim 1, which is characterized in that the microelement is by zinc sulfate, magnesium sulfate It is formed with borax.
4. complex micro organism fungicide as claimed in claim 3, which is characterized in that in the microelement, zinc sulfate, magnesium sulfate Mass ratio with borax is 1:2:1.
5. complex micro organism fungicide as described in claim 1, which is characterized in that the desiccant is bentonite, silica gel, hard charcoal One of black or attapulgite.
6. a kind of for improving the complex micro organism fungicide preparation method of crop yield and quality, which is characterized in that step is such as Under:
It 1) is 20~35 DEG C in temperature, under confined conditions, by bacillus subtilis, bacillus licheniformis, bacillusmusilaginosiengineering It is inoculated on activation medium with 4 kinds of microorganism fungus kinds of lactobacillus plantarum, after activation culture 24~48 hours, will cultivate respectively Strain respectively production fermentation medium in fermentation expand numerous 24~72 hours, activation culture and fermentation expand it is numerous during constantly Stirring, revolving speed 180rpm obtain the bacterium solution of above-mentioned 4 kinds of microorganism fungus kinds;
2) it is pre-processed the bacterium solution of the resulting 4 kinds of microorganism fungus kinds of step 1) to obtain the bacterium powder of various strains respectively, it is described Pretreatment is adsorbed for first 1:5 ratio in mass ratio with diatomite support, and then 35~40 DEG C of low temperature dryings are small to moisture again In 15%, being finally crushed to granularity is 40 mesh;After the bacterium powder of 4 kinds of microorganism fungus kinds is mixed, desiccant, micro member with crushing Plain uniformly mixing, obtains complex micro organism fungicide.
7. preparation method as claimed in claim 6, which is characterized in that the activation medium by beef extract 5g, peptone 5g, Sodium chloride 5g and water 1000mL are formulated, pH 6.5, and the strain 10% of activation is inoculated in production fermentation culture;It is described Produce the preparation method of fermentation culture are as follows: by starch 10kg, beancake powder 10kg, peptone 1kg, ammonium sulfate 1kg, biphosphate After potassium 0.5kg, yeast powder 0.1kg and calcium carbonate 5kg are mixed, it is settled to 1t with water, adjusts pH 7.5;Aerobic fermentation ventilatory capacity is 0.6~0.8V/Vmin.
8. preparation method as claimed in claim 6, which is characterized in that the activation medium and production fermentation medium difference At 121 DEG C after 30~50min of preparatory high-temperature sterilization, adds various microorganism fungus kinds and cultivated.
9. a kind of complex micro organism fungicide as described in claim 1 is improving the purposes in crop yield and quality.
10. purposes as claimed in claim 9, which is characterized in that the crops are industrial crops, including greengrocery, melon and fruit Class, fruit tree kind, tealeaves class, herbs Class, flowers class crop.
CN201811264239.4A 2018-10-29 2018-10-29 It is a kind of for improving the complex micro organism fungicide and purposes of crop yield and quality Pending CN109320345A (en)

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CN112136835A (en) * 2020-10-01 2020-12-29 菏泽嘉麟生物科技有限公司 Compound microbial agent capable of improving stress resistance of crops

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