CN111635282B - Special bacterial fertilizer for tea trees and preparation method thereof - Google Patents
Special bacterial fertilizer for tea trees and preparation method thereof Download PDFInfo
- Publication number
- CN111635282B CN111635282B CN202010543655.9A CN202010543655A CN111635282B CN 111635282 B CN111635282 B CN 111635282B CN 202010543655 A CN202010543655 A CN 202010543655A CN 111635282 B CN111635282 B CN 111635282B
- Authority
- CN
- China
- Prior art keywords
- parts
- tea trees
- tea
- pig manure
- bacterial fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
Abstract
The invention belongs to the field of tea tree fertilizers and provides a special bacterial fertilizer for tea trees and a preparation method thereof. The special bacterial fertilizer for the tea trees comprises the following raw materials in parts by weight: 5-10 parts of trichoderma asperellum TC01, 10-30 parts of pig manure, 1-5 parts of fulvic acid and 2-10 parts of charcoal, wherein the pig manure, the fulvic acid and the charcoal are mixed according to parts by weight and then inoculated into trichoderma asperellum TC01, and the special bacterial fertilizer for tea trees is obtained. The special bacterial fertilizer for the tea trees, provided by the invention, is prepared from trichoderma asperellum TC01, so that on one hand, all or part of chemical fertilizers are replaced, and the application amount of the chemical fertilizers can be reduced; on the other hand, the bacterial manure can improve the nutrient absorption rate of tea trees, improve the resistance of the tea trees and reduce the application of pesticides. The quality of the tea is improved while the ecological environment is protected, and the quality safety of the tea raw material is ensured.
Description
Technical Field
The invention relates to the technical field of tea tree fertilizers, in particular to a special bacterial fertilizer for tea trees and a preparation method thereof.
Background
Asperellum is a newly recorded trichoderma strain in China in recent years, and the development and utilization of the trichoderma strain are still in the beginning stage.
Tea trees are mostly planted in mountain barren soil, and fertilizer application can increase nutrient supply of the soil to the tea trees, so that the normal physiological metabolic process of the tea trees is maintained, and the method has important significance for improving the yield and quality of the tea trees. With the large-area popularization of clonal tea plant varieties and the pursuit of high yield of partial tea growers, the fertilizer consumption of the tea garden is gradually increased. Excessive fertilization not only increases production cost but also causes environmental problems such as soil acidification, non-point source pollution, greenhouse gas emission and the like. The research of finding a new fertilizer source to replace or partially replace chemical fertilizers is more and more important, the biological bacterial fertilizer grows along with the operation, and the application of the biological bacterial fertilizer into the soil can improve the physicochemical property of the soil, increase the content of the quick-acting nutrients in the soil and promote the absorption of crops to the nutrients.
Disclosure of Invention
The invention aims to provide a special bacterial fertilizer for tea trees and a preparation method thereof by using trichoderma asperellum TC01, which can promote the absorption of tea trees on nutrients and improve the resistance of tea trees.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 5-10 parts of trichoderma echinospora TC01, 10-30 parts of pig manure, 1-5 parts of fulvic acid and 2-10 parts of charcoal.
Preferably, the Trichoderma asperellum TC01, called Trichoderma asperellum by latin, is deposited in the common microorganism center of the china committee for culture collection management of microorganisms, and the deposition address is: the preservation date of No. 3 Xilu Beijing province of Chaoyang, China institute for scientific microbiology, is as follows: and in 2019, 1, 9 days, the preservation number is as follows: CGMCC NO: 3.19218.
preferably, the preparation method of the pig manure comprises the following steps: and mixing the organic fertilizer starter, the auxiliary materials and the pig manure, and then fermenting.
Preferably, the auxiliary materials are one or more of rice bran, corn flour, cane sugar and brown sugar.
Preferably, the mass ratio of the organic fertilizer starter to the auxiliary materials to the pig manure is 1 (6-10) to 1000-1400.
Preferably, the fermentation temperature is 25-55 ℃, the fermentation time is 18-25 days, the water content is 60-70%, and ventilation and pile turning are performed in the fermentation process.
The invention also provides a preparation method of the special bacterial fertilizer for the tea trees, which comprises the following steps: mixing the pig manure, the fulvic acid and the biochar according to the parts by weight, and adding the mixture into trichoderma asperellum TC 01.
The special bacterial fertilizer for the tea trees, provided by the invention, is prepared from trichoderma asperellum, so that on one hand, all or part of chemical fertilizers are replaced, and the application amount of the chemical fertilizers can be reduced; on the other hand, the bacterial fertilizer can improve the nutrient absorption rate of tea trees, improve the resistance and growth condition of the tea trees and reduce the application of pesticides. The quality of the tea is improved while the ecological environment is protected, and the quality safety of the tea raw material is ensured.
Drawings
FIG. 1 shows the growth of tea trees in the CB group of Experimental example 1;
FIG. 2 shows the growth of tea trees in CK group of Experimental example 1.
Detailed Description
The invention provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 5-10 parts of trichoderma echinospora TC01, 10-30 parts of pig manure, 1-5 parts of fulvic acid and 2-10 parts of charcoal.
In the invention, the special bacterial fertilizer for tea trees comprises the following raw materials in parts by weight: 5-10 parts of trichoderma echinospora TC01, preferably 5-8 parts, and more preferably 6 parts; 10-30 parts of pig manure, preferably 15-25 parts of pig manure, and further preferably 20 parts of pig manure; 1-5 parts of fulvic acid, preferably 2-4 parts, and more preferably 3 parts; 2 to 10 parts of biochar, preferably 4 to 7 parts, and more preferably 5 parts.
In the present invention, the Trichoderma asperellum TC01, preferably the latin literature name is Trichoderma asperellum, is deposited in the common microorganism center of the china committee for culture collection management of microorganisms, and the deposition date is: and in 2019, 1, 9 days, the preservation number is as follows: CGMCCNO: 3.19218.
In the invention, the preparation method of the pig manure is preferably as follows: and mixing the organic fertilizer starter, the auxiliary materials and the pig manure, and then fermenting.
In the invention, the auxiliary material is preferably one or more of rice bran, corn flour, cane sugar and brown sugar, more preferably one or more of rice bran, corn flour and cane sugar, and still more preferably a mixed auxiliary material of rice bran, corn flour and cane sugar according to a mass ratio of 1:1: 2.
In the invention, the mass ratio of the organic fertilizer starter to the auxiliary materials to the pig manure is preferably 1 (6-10): 1000-1400, more preferably 1 (7-9): 1100-1300, and still more preferably 1:8: 1200.
In the invention, the fermentation temperature is preferably 25-55 ℃, more preferably 30-50 ℃, and still more preferably 48 ℃.
In the present invention, the water content of the fermentation mixture is preferably 60 to 70%, and more preferably 65%.
In the invention, ventilation and pile turning are preferably carried out in the fermentation process, and the pile turning is preferably carried out for 2-3 times when the fermentation temperature reaches 65 ℃, and ventilation is carried out simultaneously;
in the present invention, the fermentation time is preferably 18 to 25 days, more preferably 20 to 22 days, and still more preferably 21 days.
The invention also provides a preparation method of the special bacterial fertilizer for the tea trees, which preferably comprises the following steps: mixing the pig manure, the fulvic acid and the biochar according to the parts by weight, and adding the mixture into trichoderma asperellum TC 01.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The embodiment provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 6 parts of trichoderma echinospora TC01, 20 parts of pig manure, 3 parts of fulvic acid and 5 parts of charcoal. The fermentation method of the pig manure comprises the steps of uniformly mixing an organic fertilizer starter, auxiliary materials (the auxiliary materials are mixed auxiliary materials of rice bran, corn flour and cane sugar in a mass ratio of 1:1: 2) and the pig manure in a mass ratio of 1:8:1200, controlling the water content to be 65%, and fermenting for 21 days at a fermentation temperature of 48 ℃. Turning the pile 2 times when the fermentation temperature reaches 65 ℃, and ventilating simultaneously.
And (3) mixing the fermented pig manure with fulvic acid and charcoal, adding trichoderma asperellum TC01, and uniformly mixing to obtain the special bacterial manure for the tea trees. The bacterial manure is a complete biological bacterial manure and can be directly applied to tea trees.
Example 2
The embodiment provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 5 parts of trichoderma echinospora TC01, 25 parts of pig manure, 2 parts of fulvic acid and 10 parts of charcoal. The fermentation method of the pig manure comprises the steps of uniformly mixing an organic fertilizer starter, auxiliary materials (the auxiliary materials are mixed auxiliary materials of rice bran, wheat bran and corn flour in a mass ratio of 1:1: 1) and pig manure in a mass ratio of 1:7:1300, controlling the water content to be 60%, and fermenting for 25 days at the fermentation temperature of 25 ℃. Turning over the pile 3 times when the fermentation temperature reaches 65 ℃, and ventilating simultaneously.
And (3) mixing the fermented pig manure with fulvic acid and charcoal, adding trichoderma asperellum TC01, and uniformly mixing to obtain the special bacterial manure for the tea trees. The bacterial manure is a complete biological bacterial manure and can be directly applied to tea trees.
Example 3
The embodiment provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 10 parts of trichoderma echinospora TC01, 30 parts of pig manure, 4 parts of fulvic acid and 4 parts of charcoal. The fermentation method of the pig manure comprises the steps of uniformly mixing an organic fertilizer starter, auxiliary materials (the auxiliary materials are mixed auxiliary materials of rice bran, brown sugar and cane sugar in a mass ratio of 2:1: 1) and the pig manure in a mass ratio of 1:6:1000, controlling the water content to be 70%, and fermenting for 20 days at the fermentation temperature of 50 ℃. Turning the pile 2 times when the fermentation temperature reaches 65 ℃, and ventilating simultaneously.
And (3) mixing the fermented pig manure with fulvic acid and charcoal, adding trichoderma asperellum TC01, and uniformly mixing to obtain the special bacterial manure for the tea trees. The bacterial manure is a complete biological bacterial manure and can be directly applied to tea trees.
Example 4
The embodiment provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 8 parts of trichoderma echinospora TC01, 15 parts of pig manure, 1 part of fulvic acid and 7 parts of charcoal. The fermentation method of the pig manure comprises the steps of uniformly mixing an organic fertilizer starter, auxiliary materials (the auxiliary materials are mixed auxiliary materials of bran and corn flour according to the mass ratio of 1: 1) and the pig manure according to the mass ratio of 1:10:1400, controlling the water content to be 65%, and fermenting at 55 ℃ for 22 days. Turning the pile 3 times when the fermentation temperature reaches 65 ℃, and ventilating simultaneously.
And (3) mixing the fermented pig manure with fulvic acid and charcoal, adding trichoderma asperellum TC01, and uniformly mixing to obtain the special bacterial manure for the tea trees. The bacterial manure is a complete biological bacterial manure and can be directly applied to tea trees.
Example 5
The embodiment provides a special bacterial fertilizer for tea trees, which comprises the following raw materials in parts by weight: 5 parts of trichoderma echinospora TC01, 10 parts of pig manure, 5 parts of fulvic acid and 2 parts of charcoal. The fermentation method of the pig manure comprises the steps of uniformly mixing an organic fertilizer starter, an auxiliary material (rice bran as the auxiliary material) and the pig manure according to the mass ratio of 1:9:1100, controlling the water content to be 70%, and fermenting for 18 days at the fermentation temperature of 30 ℃. Turning the pile 3 times when the fermentation temperature reaches 65 ℃, and ventilating simultaneously.
And (3) mixing the fermented pig manure with fulvic acid and charcoal, adding trichoderma asperellum TC01, and uniformly mixing to obtain the special bacterial manure for the tea trees. The bacterial manure is a complete biological bacterial manure and can be directly applied to tea trees.
Experimental example 1
The tea tree specific bacterial manure prepared in example 1 was applied to tea tree (CB) and a Control (CK) was set.
The application method comprises the following steps: in the middle and late 10 months, experimental group CB: applying a tea formula fertilizer and a special tea tree bacterial fertilizer according to a ratio of 7:3, wherein the application amount is 35 kg/mu of the tea formula fertilizer and 15 kg/mu of the special tea tree bacterial fertilizer; control CK: only applying the tea formula fertilizer according to 50 kg/mu. And then topdressing is carried out on the experimental group and the control group in the last 1 month of the next year, and 15kg of urea is topdressed per mu. The formula fertilizer for the tea comprises: N-P2O5-K2O18: 8:12 (available from shizhu agriculture science and technology ltd, mush, north).
Sampling soil samples before top dressing in 1 month of the next year and before picking tea in 3 months of spring, and detecting the change of soil nutrient content, as shown in table 1:
TABLE 1 variation of soil nutrient content (unit: mg/kg)
The data are analyzed statistically, and p is less than 0.05, so that the statistical significance is achieved.
As can be seen from Table 1, the application of the bacterial manure of the invention to tea trees increases the content of available nutrients in soil, and the trichoderma asperellum TC01 is beneficial to the transportation and absorption of nutrients in soil by tea trees.
Experimental example 2
Fertilizing according to the method of experimental example 1, after topdressing tea leaves in 1 month later, inoculating anthrax pathogenic bacteria, investigating disease index after inoculating for 10 days, and calculating disease index and relative prevention effect of tea trees according to disease grading standard.
The disease grading criteria are as follows:
level 0: the leaves are healthy and disease-free;
level 1:1 to 25 percent of leaf area is attacked;
and 2, stage: 26 to 50 percent of leaf area;
and 3, level: 51 to 75 percent of leaf area is attacked;
4, level: more than 76 percent of the area of the leaf blade is yellow and festered.
Disease index [ Σ (disease number × number of diseased leaves at this level)/(highest disease number × total investigation number) ] ×%;
relative control effect (%) [ (CK group leaf disease index-CB group leaf disease index)/CK group leaf disease index ] ×%.
The investigation and calculation result shows that the disease index of the CB group is 35.6 percent, the disease index of the CK group is 60.2 percent, the relative control effect is 40.86 percent, the resistance of the tea tree is improved, and the application of pesticides is reduced.
Meanwhile, the growth of tea trees is promoted by applying the special bacterial fertilizer for tea trees, and as can be seen from the graph 1-2, the tea branches and leaves applied with the special bacterial fertilizer for tea trees are flourishing, the leaves are tender green, and the tea trees in a control group are short and the leaves are yellow.
The growth of tea trees in the CB and CK groups was compared in this experiment, as shown in Table 2; the fresh weight of the plant height, the stem thickness, the underground part and the overground part of the tea tree are all increased compared with CK.
TABLE 2 comparison of growth of tea plants
Therefore, the special bacterial fertilizer for the tea trees can promote the growth of the tea trees and improve the quality of the tea.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (2)
1. The special bacterial fertilizer for the tea trees is characterized by comprising the following raw materials in parts by weight: 6 parts of trichoderma asperellum TC01, 20 parts of pig manure, 3 parts of fulvic acid and 5 parts of charcoal;
the trichoderma asperellum TC01 is named as Trichoderma aasperellum in Latin, is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and has the preservation date as follows: and in 2019, 1, 9 days, the preservation number is as follows: CGMCC NO: 3.19218, respectively;
the preparation method of the pig manure comprises the following steps: mixing an organic fertilizer starter, auxiliary materials and pig manure and then fermenting;
the auxiliary materials are rice bran, corn flour and cane sugar which are mixed according to the mass ratio of 1:1: 2;
the mass ratio of the organic fertilizer starter to the auxiliary materials to the pig manure is 1:8: 1200;
the fermentation temperature is 48 ℃, the fermentation time is 21 days, the water content is 65%, and ventilation and pile turning are carried out in the fermentation process.
2. The preparation method of the bacterial fertilizer special for tea trees, which is disclosed by claim 1, is characterized by comprising the following steps of: mixing the pig manure, the fulvic acid and the biochar according to the parts by weight, and adding the mixture into trichoderma asperellum TC 01.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010543655.9A CN111635282B (en) | 2020-06-15 | 2020-06-15 | Special bacterial fertilizer for tea trees and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010543655.9A CN111635282B (en) | 2020-06-15 | 2020-06-15 | Special bacterial fertilizer for tea trees and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111635282A CN111635282A (en) | 2020-09-08 |
| CN111635282B true CN111635282B (en) | 2022-04-22 |
Family
ID=72325689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010543655.9A Active CN111635282B (en) | 2020-06-15 | 2020-06-15 | Special bacterial fertilizer for tea trees and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111635282B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232551A (en) * | 2014-09-29 | 2014-12-24 | 山东苏柯汉生物工程股份有限公司 | Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer |
| CN106929431A (en) * | 2017-02-16 | 2017-07-07 | 德强生物股份有限公司 | A kind of straw decomposing inoculant and its preparation method and application |
| CN108503466A (en) * | 2018-06-29 | 2018-09-07 | 贵州大学 | A kind of tea tree special bio bacterial manure and the preparation method and application thereof |
| CN109251098A (en) * | 2018-10-31 | 2019-01-22 | 周口师范学院 | A kind of quick returning to the field method of straw bacterial manure |
| CN109749943A (en) * | 2019-03-06 | 2019-05-14 | 重庆市农业科学院 | A kind of Trichoderma asperellum and its application |
-
2020
- 2020-06-15 CN CN202010543655.9A patent/CN111635282B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232551A (en) * | 2014-09-29 | 2014-12-24 | 山东苏柯汉生物工程股份有限公司 | Pig-chicken excrement fermenting compound bacteria, preparation method and method of utilizing compound bacteria to ferment and prepare organic fertilizer |
| CN106929431A (en) * | 2017-02-16 | 2017-07-07 | 德强生物股份有限公司 | A kind of straw decomposing inoculant and its preparation method and application |
| CN108503466A (en) * | 2018-06-29 | 2018-09-07 | 贵州大学 | A kind of tea tree special bio bacterial manure and the preparation method and application thereof |
| CN109251098A (en) * | 2018-10-31 | 2019-01-22 | 周口师范学院 | A kind of quick returning to the field method of straw bacterial manure |
| CN109749943A (en) * | 2019-03-06 | 2019-05-14 | 重庆市农业科学院 | A kind of Trichoderma asperellum and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111635282A (en) | 2020-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101863695B (en) | Microbial organic fertilizer and preparation method thereof | |
| AU2008331378B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN108976030B (en) | Special fertilizer for strawberries by taking insect manure as matrix and preparation method and application thereof | |
| CN104478550B (en) | It is a kind of suitable for tea grounds earthworm excrement matrix of tomato seedling and preparation method thereof | |
| CN109400372B (en) | Biochar soil improvement organic fertilizer and preparation method thereof | |
| CN107216227A (en) | A kind of apple special bio charcoal base microbe bacterial manure and preparation method thereof | |
| CN109796289A (en) | It is a kind of using oil-tea camellia husks as bio-fertilizer of raw material and preparation method thereof | |
| CN110590421A (en) | Drought-resisting and water-retaining soil conditioner for hilly and mountainous areas and preparation method thereof | |
| CN103992184A (en) | Bio-organic fertilizer | |
| CN103420730B (en) | Hole-tray vegetable cultivation substrate | |
| CN109479671A (en) | A kind of domesticating and cultivating matrix and preparation method thereof improving tuber of pinellia tissue culture shoot survival percent | |
| CN105272421A (en) | Agricultural culturing medium, producing method thereof and application thereof | |
| CN106416799A (en) | Vegetable culture medium | |
| CN113604224A (en) | Composite improver for mountainous area barren soil and use method thereof | |
| CN112457125A (en) | Banana wilt prevention bio-organic fertilizer and preparation method and application thereof | |
| CN110972891B (en) | Ecological composite culture medium taking garden waste as main raw material and application thereof | |
| CN111499430A (en) | Humic acid compound fertilizer for improving tobacco-planting soil and preparation method thereof | |
| CN114097571A (en) | Method for fermentation and matrixing cyclic utilization of corn straw | |
| CN107099490B (en) | Biological agent for treating crop straws | |
| CN105624062B (en) | Eupatorium adenophorum organic fertilizer solid microbial inoculum, preparation method and application in organic fertilizer production | |
| CN111635282B (en) | Special bacterial fertilizer for tea trees and preparation method thereof | |
| CN109924104B (en) | Seedling culture matrix composition of myriophyllum viridis dry matter, preparation method and application | |
| CN103880551B (en) | Utilize the method for paper mill sludge quick fermentation production seedling medium | |
| CN107365199B (en) | Method for preparing special vinasse bio-organic fertilizer for mosaic disease-preventing flue-cured tobacco by using Luzhou vinasse | |
| CN107298596A (en) | A kind of method that straw-returning is carried out using biochemical technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |