CN112457125A - Banana wilt prevention bio-organic fertilizer and preparation method and application thereof - Google Patents
Banana wilt prevention bio-organic fertilizer and preparation method and application thereof Download PDFInfo
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- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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Abstract
The invention relates to the technical field of agricultural soil biofertilizers, and particularly discloses a banana vascular wilt preventing bio-organic fertilizer and a preparation method and application thereof. The biological organic fertilizer for preventing banana wilt is prepared by the following method: mixing pig manure, cassava residues, banana stem leaves, banana peels, molasses fermentation powder and earthworm soil according to the mass ratio of 50-55: 25-30: 3-5: 15-20 of dry substances, composting and fermenting to prepare an organic fertilizer semi-finished product, inoculating a compound bacterium consisting of bacillus subtilis, paenibacillus polymyxa and Mortierella elongata, and preparing the biological organic fertilizer for preventing banana wilt through secondary fermentation. The high-quality organic fertilizer disclosed by the invention is found in banana planting application, and not only can improve the soil for banana growth and improve the nutrient utilization rate, but also can effectively prevent and control diseases and insect pests such as banana wilt and the like and improve the banana yield.
Description
Technical Field
The invention belongs to the technical field of agricultural soil biofertilizers, and particularly relates to a banana vascular wilt preventing biological organic fertilizer as well as a preparation method and application thereof.
Background
The banana vascular wilt is a typical fungal soil-borne disease for damaging vascular bundles caused by fusarium oxysporum cubeba specialized infection, wherein the damage of No. 4 physiological race is the most serious, and the banana vascular wilt occurs in almost all banana production areas in the world. At present, banana wilt has entered the rapid expansion stage, seriously threatens the healthy development of banana industry, and seeks an effective prevention and treatment method, which is an important concern in the main production area of bananas. The use of a large amount of chemical agents pollutes the environment, destroys the ecological balance and reduces the quality of bananas; the cleaning effect of the banana garden soil is poor; the short-term crop rotation is possible to spread diseases; the transgenic banana varieties have less application in production. Among the numerous control methods, biological control is recognized as a relatively safe, environmentally friendly and sustainable control measure for agricultural production. Scholars at home and abroad screen out certain antagonistic fungi, bacteria and actinomycetes which have an inhibiting effect on banana wilt, but a large number of researches show that the purpose of efficiently inhibiting plant diseases and insect pests for a long time can be achieved by applying a certain antagonistic bacterium only rarely; in addition, the antagonistic bacteria needs to be added into the soil by a proper carrier, and the antagonistic bacteria cannot survive and have poor prevention and treatment effects after being added into the soil by no proper carrier.
Disclosure of Invention
The invention aims to provide a biological organic fertilizer for preventing banana vascular wilt, which is characterized in that compound functional bacteria and the organic fertilizer are jointly applied, so that the banana vascular wilt is well prevented and controlled, the pollution of chemical fertilizers and pesticides to the ecological environment is reduced, the soil fertility is improved, the growth of banana plants is promoted, and the yield and the quality of bananas are improved.
In order to achieve the purpose, the invention provides a biological organic fertilizer for preventing banana wilt, which is prepared by adding composite bacterial liquid of Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX for secondary fermentation by taking cassava residue, pig manure, banana stem and leaf crushed material, banana peel crushed material, molasses fermentation powder and earthworm soil as main raw materials. The preparation method comprises the following steps:
(1) preparing materials: mixing the raw materials of pig manure, cassava residues, banana tree stems and leaves, banana peel, molasses baking powder and earthworm soil according to the mass of dry substances of 50-55: 25-30: 3-5;
(2) primary fermentation: uniformly mixing the prepared materials, and carrying out stack retting fermentation for 6-7 d to prepare an organic fertilizer semi-finished product;
(3) inoculating functional bacteria: after the water content of the semi-finished organic fertilizer is adjusted to be 40-50%, adding a compound bacterial liquid, wherein the compound bacterial liquid comprises Bacillus subtilis (Bacillus subtilis), Paenibacillus polymyxa (Paenibacillus polymyxa) and Mortierella elongata (Mortierella elongata SX), and mixing the compound bacterial liquid and the Mortierella elongata SX according to the proportion that the colony forming unit number is 1: 0.8-1.2: 1.0-1.4;
(4) and (3) secondary fermentation: and inoculating the compound bacterial liquid to the semi-finished organic fertilizer, and performing secondary fermentation, wherein the material is turned for 1 time every 2 days in the fermentation process, the material is turned for 3-5 times totally, and the preparation of the biological organic fertilizer can be completed after 7-10 days.
The earthworm soil is soil after earthworms are bred for 2-4 months, and the earthworm soil is obtained by adding 15-18% of crustacean shell powder crushed aggregates, 4.0-8.0% of potassium ore powder, 1.5-4.5% of phosphate rock and 0.01-0.02% of sodium borate into the earthworm breeding soil.
The invention provides a biological organic fertilizer, which is characterized in that: the water content of the biological organic fertilizer is 22-25%, the pH value is 5.0-6.0, and the total viable bacteria activity number is more than 0.2 multiplied by 109cfu/g, 47-51% of organic matter, 5-6.5% of total nitrogen and P2O51.6-1.9% of total nitrogen％，K2O 12.5％～14％。
Compared with the prior art, the invention has the following beneficial effects:
1. the cassava residues are used as the basic raw materials of the biological organic fertilizer, the advantages of Guangxi are combined, the cassava residues are changed into valuable materials, the application approach of the cassava residues is expanded, and meanwhile, the cassava residues are high in organic matter content and free of heavy metal, so that the banana quality is improved; in addition, the cassava dregs contain less harmful bacteria and pathogens, and the cassava dregs are used as the bio-organic fertilizer for banana planting, so that the risk of banana plant diseases and insect pests is reduced.
2. The banana stem leaf crushed material and the banana peel crushed material have almost all necessary nutrients in the banana growth process, the banana stem leaf crushed material and the banana peel crushed material are used as organic fertilizer raw materials to be beneficial to balance of all nutrients in the banana planting process, conditions are provided for sufficient growth of banana trees, on the other hand, the banana stem leaf crushed material and the banana peel crushed material are used as organic fertilizer raw materials, waste such as banana stem leaves and banana peels is reasonably utilized, and the problem of aspects of environmental sanitation and the like is reduced.
3. The molasses fermentation powder and the earthworm soil are added into the microbial organic fertilizer to provide a good growth and propagation environment for the inoculated beneficial strains; meanwhile, the molasses fermentation powder is rich in humic acid, yellow rot and other substances, and the earthworm soil is rich in organic matters, fluffy and porous and has a remarkable improvement effect on the planting soil; the earthworm soil is obtained by adding shell powder crushed aggregates of crustaceans, phosphorite powder, potassium ore powder and sodium borate into earth for raising earthworms, the raw materials are cheap and easy to obtain, the content of components such as potassium and phosphorus is particularly increased according to the demand of nutritional components for banana growth, the preparation is reasonable, the nutritional components of the fertilizer formed after the earthworm phagocytosis and digestion have strong pertinence to the banana growth, and the components are easy to absorb; the earthworm soil formed by adding the crustacean shell crushed material into the earth for feeding earthworms is rich in chitin, has certain inhibiting effect on some soil viruses and insect pests, is beneficial to reducing the use of chemical pesticides, and simultaneously, the chitin in the earthworm soil can also promote the growth of banana roots, induce the disease resistance and stress resistance of plants and promote the absorption and utilization of nutrient components in the soil;
4. the invention screens out the compound bacterial liquid which can synergistically inhibit banana vascular wilt, and combines the compound bacterial liquid with organic fertilizer to prepare the bio-organic fertilizer, wherein the bio-organic fertilizer is applied to banana planting: compared with conventional fertilization, the yield of bananas per mu is obviously increased after application, the banana seed fertilizer has a good prevention and control effect on germs such as banana wilt and the like, and the banana soil environment can be effectively improved.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
Preparation of bacterial liquid
Taking rhizosphere soil of a healthy banana garden as a research object, separating, purifying, screening to obtain Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX, activating, respectively selecting 1-ring activated bacterial colonies, inoculating into 1000ml of LB culture solution, and culturing in a constant-temperature oscillation incubator at 28 ℃ for 24h at 160rpm to form a bacterial solution.
Several 100mL conical flasks were prepared, and 20mL of PDA (pH adjusted to 7) culture medium was poured into the 100mL conical flasks, and the flasks were sterilized at high temperature after being filled.
Inoculating the cultured three functional bacteria into a 100mL conical flask filled with 20mL of PDA culture solution according to the proportion of 1:100, and culturing for 48h at 30 ℃ and the shaking table speed of 120rpm to obtain a seed solution.
Example 2
Test of prevention and treatment effect of bacterial strain on banana wilt
1.1 materials and methods
1.1.1 test materials
Banana test: the tested banana variety is the bacon banana and 6 leaves.
Test microbial inoculum: FOC4 pathogenic bacterium, spore concentration 106cfu/ml; antagonistic bacteria Bacillus subtilis KC with the thallus concentration of 107cfu/ml; antagonistic bacterium Paenibacillus polymyxa DN with the thallus concentration of 107cfu/ml; mortierella elongataCB, cell concentration 107cfu/ml; the composite bacteria FH1 (Bacillus subtilis, Paenibacillus polymyxa) and Mortierella elongata SX (Mortierella elongata SX) are mixed according to the proportion that the number of colony forming units is 1:0.8: 1), and the thallus concentration is 107cfu/ml; the composite bacteria FH2 (Bacillus subtilis, Paenibacillus polymyxa) and Mortierella elongata (Mortierella elongata SX) are mixed according to the proportion that the number of colony forming units is 1:1.2: 1.4), and the thallus concentration is 107cfu/ml；
The composite bacteria CD (Bacillus subtilis) and Paenibacillus polymyxa (Paenibacillus polymyxa) are mixed according to the proportion that the number of colony forming units is 1:1, and the thallus concentration is 107cfu/ml)。
Test soil: the soil is collected from a healthy banana garden soil of a test base of the Taiping town Xinliancun in the Wuming district, and each bucket is 7.5 kg.
Test time: transplanting at 25 months 8 in 2016, and spraying 3 times every 20 days a ternary compound fertilizer (N: P: K: 15: 15: 15) with concentration of 2.5%. Carrying out root injury treatment on 1 day at 11 months, simultaneously carrying out FOC4 strain inoculation on leaves, and respectively inoculating 100ml of experimental microbial inoculum with the concentration of 10 strains to the treatment 2-7 after 10 days7cfu/ml。
1.1.2 test design
The test set 6 treatments, each of which planted 30 pots, were randomly arranged. The treatment is respectively as follows:
treatment 1: control, 100ml of pathogenic bacteria inoculated with FOC4 (spore concentration 10)6cfu/ml)
And (3) treatment 2: 100ml of pathogenic bacteria FOC4 + 100ml of bacillus subtilis KC
And (3) treatment: 100ml of pathogenic bacteria FOC4 + DN 100ml of paenibacillus polymyxa
And (4) treatment: 100ml of pathogenic bacteria FOC4 + 100ml of Mortierella elongata CB
And (4) treatment 5: 100ml pathogenic bacteria FOC4 inoculation + composite bacteria FH 1100 ml
And (6) treatment: 100ml pathogenic bacteria FOC4 inoculation + composite bacteria FH 2100 ml
And (7) treatment: 100ml of FOC4 pathogenic bacteria and 100ml of composite bacteria CD
Test site: glass greenhouse of the test base of Guangxi academy of agricultural sciences.
1.2 prevention and treatment Effect of the bacteria on the banana wilt disease of the banana plants
As shown in Table 1, different treatments had different effects on disease index and relative prevention and control effects of banana vascular wilt. The disease indexes of 7 treatments are represented by that composite bacteria FH1 is more than composite bacteria FH2 is more than composite bacteria CD is more than Bacillus subtilis KC is more than Paenibacillus polymyxa DN is more than Mortierella elongata CB is less than a control. The disease index is 0.6 by applying the bacillus subtilis, and the prevention and treatment effect of the banana vascular wilt reaches 27.97 percent; the disease index of the paenibacillus polymyxa is 0.733, and the control effect of the banana vascular wilt is 19.9%; the disease index of the Mortierella elongata is 1.167, and the prevention and treatment effect of the banana vascular wilt is 12 percent; the disease index of the compound bacteria FH1 is 0.333, and the prevention and treatment effect of the banana vascular wilt is 60%; the disease index of the compound bacteria FH2 is 0.367, the control effect of the banana vascular wilt is 55.9 percent obvious, and the effect of reducing the banana vascular wilt by applying the compound bacteria consisting of the bacillus subtilis, the paenibacillus polymyxa and the mortierella elongata is obviously better than that of independently using the bacillus subtilis, the paenibacillus polymyxa, the mortierella elongata or the compound bacteria consisting of only the bacillus subtilis and the paenibacillus polymyxa.
TABLE 1 situation of the strains on the prevention and control of banana vascular wilt
Example 3
1. Preparation of bio-organic fertilizer 1
(1) Preparation of organic fertilizer semi-finished product
Pig manure, cassava residue, banana stem and leaf crushed material, banana peel crushed material, molasses fermentation powder and earthworm soil are used as main raw materials, the main raw materials are mixed according to the mass ratio of 50:25:25:25:3, and the mixture is piled and fermented for 7 days to prepare the semi-finished organic fertilizer.
The earthworm soil is soil for breeding earthworms for 2-4 months, and the earthworm soil is obtained by adding 15-18% of crustacean shell powder crushed aggregates, 4.0-8.0% of potassium ore powder, 1.5-4.5% of phosphate rock and 0.01-0.02% of sodium borate into the earthworm breeding soil.
(2) Fermentation culture
After the water content of the semi-finished organic fertilizer is adjusted to 40%, seed liquids of Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX are respectively mixed according to the proportion that the number of community units is 1:0.8:1, and then added for secondary fermentation. The material is turned over for 1 time every 2 days in the fermentation process, the material is turned over for 5 times totally, and the preparation of the bio-organic fertilizer can be finished after 7 days, so that the total bacteria activity is more than 0.2 multiplied by 109cfu/g。
(3) Analysis of characteristics of finished product of bio-organic fertilizer 1
Through detection, the prepared bio-organic fertilizer contains 24.96% of water, 46.88% of organic matters, 6.4% of total nitrogen and P2O51.61％，K2O12.5%, pH 5.15, effective viable count is 0.81 hundred million cfu/g.
2. Preparation of bio-organic fertilizer 2
(1) Preparation of organic fertilizer semi-finished product
Pig manure, cassava residue, banana stem and leaf crushed material, banana peel crushed material, molasses fermentation powder and earthworm soil are used as main raw materials, the main raw materials are mixed according to the mass ratio of 55:30:30:30:5, and the mixture is piled and fermented for 6 days to prepare the semi-finished organic fertilizer.
The earthworm soil is soil for breeding earthworms for 2-4 months, and the earthworm soil is obtained by adding 15-18% of crustacean shell powder crushed aggregates, 4.0-8.0% of potassium ore powder, 1.5-4.5% of phosphate rock and 0.01-0.02% of sodium borate into the earthworm breeding soil.
(2) Fermentation culture
After the water content of the semi-finished organic fertilizer is adjusted to 50%, seed liquids of Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX are respectively mixed according to the proportion that the number of community units is 1:1.2:1.4, and then added for secondary fermentation. In the fermentation process, the material is turned for 1 time every 2 days,the preparation of the bio-organic fertilizer can be finished after 10 days after 3 times of material turning, so that the total bacteria activity is more than 0.2 multiplied by 109cfu/g。
(3) Analysis of characteristics of finished bio-organic fertilizer 2
Through detection, the prepared bio-organic fertilizer contains 22.04% of water, 50.97% of organic matters, 5.1% of total nitrogen, P2O51.87%, K2O 13.8.8%, and has the pH value of 5.88, and the effective viable count is 0.81 hundred million cfu/g.
Example 4
Use of bio-organic fertilizer
1 materials and methods
The banana variety to be tested was Brazil banana.
The test biological organic fertilizer comprises the following components: a sample biological organic fertilizer and a conventional fertilizer for test.
Test site: banana planting bases of Yufa agriculture science and technology development Limited company in the Taiping town of the Wuming district in Nanning city, and the like are provided with an old planting area with blight and a new planting area without blight. Each area is provided with 3 treatments, each treatment comprises 20 rows, each row comprises 40 plants, the planting is carried out for 800 plants in total, and the row spacing of the plants is 2m multiplied by 2.5 m. The 2 test areas were fertilized 1 st on day 26 in 4 months and 2 nd on day 22 in 7 months in 2016.
2 design of the experiment
(1) The old planting area (third generation seedling) experimental design is 3 treatments, which are respectively:
treatment 1: the sample biological organic fertilizer 1 (the effective viable count is more than or equal to 0.20 hundred million cfu/g, the organic matter is more than or equal to 40 percent, and the fertilizer is applied in two times), which is hereinafter referred to as LF 1.
And (3) treatment 2: the sample biological organic fertilizer 2 (the effective viable count is more than or equal to 0.20 hundred million cfu/g, the organic matter is more than or equal to 40 percent, and the fertilizer is applied in two times), which is hereinafter referred to as LF 2.
And (3) treatment: comparison (conventional fertilization), hereinafter abbreviated as LCK.
The old planting areas LF1 and LF2 are 12 jin/plant, wherein 6 jin/plant is used as a base fertilizer (3 months per year), and 6 jin/plant is additionally fertilized in 7-8 months; LCK treatments (control, conventional fertilization method), field management was consistent for each treatment.
(2) New planting area (first generation seedling)
Experimental design 3 treatments were respectively:
treatment 1: sample biological organic fertilizer 1 (the effective viable count is more than or equal to 0.20 hundred million cfu/g, the organic matter is more than or equal to 40 percent, and the fertilizer is applied twice), which is hereinafter referred to as XF 1.
And (3) treatment 2: sample biological organic fertilizer 2 (effective viable count is more than or equal to 0.20 hundred million cfu/g, organic matter is more than or equal to 40 percent, fertilization is carried out twice), hereinafter referred to as XF 2.
And (3) treatment: control (conventional fertilization). Hereinafter referred to as XCK.
The XF1 and XF2 of the new planting areas are 8 jin/plant, wherein 5 jin/plant is used as a base fertilizer, 3 jin/plant is additionally fertilized in 7-8 months, and XCK treatment (contrast and conventional fertilization method) is carried out, and the field management of each treatment is consistent.
3. Investigation of prevention and control of banana wilt in old planting area by different treatments
As can be seen from the table 2, when 200 plants are investigated and treated by the prevention and control test by applying the bio-organic fertilizer, the incidence rates of the banana vascular wilt are respectively 14% and 12%, the incidence rates are reduced by 12% and 14% compared with the incidence rate of the control treatment (LCK), and the reduction rate reaches 46% and 54%.
TABLE 2 Banana wilt occurrence in old planting area
2. Effect of different treatments on Banana yield in old growing areas
As can be seen from the table 3, the bio-organic fertilizer is applied to the old planting area, the yield of each banana plant is 52.3 jin and 52.5 jin respectively, the reduced yield per mu is 3140.4 and 3152.7 kg, the yield per mu is increased by 16.21 percent and 16.66 percent compared with the control, and the increase effect is obvious.
TABLE 3 influence of bio-organic fertilizer on banana yield in old planting area
5 Effect of different treatments on physicochemical Properties of soil in old planting areas
As can be seen from Table 4, the application of the bio-organic fertilizer significantly increases the contents of the soil available nitrogen, available phosphorus, available potassium, exchangeable calcium, exchangeable magnesium, organic matters, soil total nitrogen and total phosphorus.
TABLE 4 influence of the application of the bio-organic fertilizer on the physicochemical properties of the soil in the old planting areas
| Treatment of | LF1 | LF2 | LCK |
| All N (%) | 0.274 | 0.257 | 0.156 |
| All P (%) | 0.892 | 0.906 | 0.361 |
| Whole K (%) | 0.174 | 0.186 | 0.148 |
| Speed N (mg/kg) | 71 | 74 | 62 |
| Speed P (mg/kg) | 131 | 129 | 68 |
| Speed K (mg/kg) | 503 | 511 | 302 |
| pH | 7.35 | 7.24 | 7.06 |
| Organic matter (g/kg) | 65.7 | 63.5 | 37.5 |
| Exchangeable Ca (cmol/kg) | 12.81 | 12.94 | 11.88 |
| Exchangeable mg (cmol/kg) | 0.31 | 0.33 | 0.13 |
6 influence of different treatments on prevention and control of banana wilt in new planting area
As can be seen from Table 5, no banana wilt plants were found in any of the 200 investigated treated with the bio-organic fertilizer, while 2 diseased plants were found in the control treatment, respectively, with a 4% incidence rate. Therefore, compared with the old planting area, the incidence rate of banana vascular wilt in the new planting area is obviously reduced, and the banana vascular wilt planted year by year is increased. From the table data, the biological organic fertilizer has better effect on preventing and controlling the banana wilt.
TABLE 5 occurrence of banana vascular wilt in newly planted area
7 Effect of different treatments on Banana yield in New plantations
As can be seen from Table 6, when the bio-organic fertilizer is applied to the new planting area, the yield of each banana plant is 53.8 and 54.1 jin respectively, the reduced yield per mu is 3228.0 and 213.4 kg, and compared with the control, the yield of each mu is increased by 6.41 percent and 7.03 percent, and the yield increasing effect of the bio-organic fertilizer is obvious.
TABLE 6 influence of bio-organic fertilizer on banana yield in new planting area
8 Effect of different treatments on physicochemical Properties of soil in New planting areas
As can be seen from Table 7, the application of the bio-organic fertilizer increases the total nitrogen, total phosphorus, quick-acting potassium, quick-acting phosphorus content, organic matter content and the like in the soil slightly reduced, but the levels are equivalent to the levels before the application of the fertilizer.
TABLE 7 Effect of the application of the Bio-organic fertilizers on the physicochemical Properties of the soil in the New planting areas
In conclusion, the biological organic fertilizer has obvious effects of preventing and controlling the blight of the bananas, improving the physical and chemical properties of the soil and obviously increasing the acre yield of the bananas.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (9)
1. The biological organic fertilizer for preventing banana wilt is characterized by being prepared by adding compound bacteria for secondary fermentation by using pig manure, cassava dregs, banana stem and leaf crushed materials, banana peel crushed materials, molasses fermentation powder and earthworm soil as main raw materials, wherein the water content of the biological organic fertilizer is 22-25%, the pH value of the biological organic fertilizer is 5.0-6.0, and the total viable bacteria activity is more than 0.2 multiplied by 109cfu/g, 48-51% of organic matter, 5-6.5% of total nitrogen and P2O5 1.6%~1.9%，K2O 12.5%~14%。
2. The biological organic fertilizer for preventing banana vascular wilt according to claim 1, wherein the mass ratio of the pig manure, the cassava residue, the banana stem and leaf crushed material, the banana peel crushed material, the molasses fermentation powder and the earthworm soil is 50-55: 25-30: 3-5: 15-20.
3. The bio-organic fertilizer for preventing banana wilt disease according to claim 1, wherein the compound bacteria are prepared by mixing bacillus subtilis, paenibacillus polymyxa and mortierella elongata according to the proportion of 1: 0.8-1.2: 1.0-1.4 of the number of colony forming units.
4. The bio-organic fertilizer for preventing banana vascular wilt according to claim 1 or 4, wherein the bacillus subtilis, paenibacillus polymyxa and mortierella elongata are obtained by separating, identifying and activating rhizosphere soil of healthy banana plants and then performing fermentation culture.
5. The biological organic fertilizer for preventing banana vascular wilt according to claim 1, wherein the earthworm soil is soil after earthworms are bred for 2-4 months.
6. The bio-organic fertilizer for preventing banana wilt disease as claimed in claim 1, wherein said earthworm soil is obtained by adding crustacean shell powder crushed aggregates, potassium ore powder, phosphate rock powder and sodium borate into earth for breeding earthworms.
7. The bio-organic fertilizer for preventing banana wilt disease as claimed in claim 6, wherein the crustacean shell powder crushed aggregates, the potassium ore powder, the phosphate ore and the sodium borate respectively account for 15-18%, 4.0-8.0%, 1.5-4.5% and 0.01-0.02% of the soil mass for breeding earthworms.
8. The bio-organic fertilizer for preventing banana wilt disease according to claim 1, characterized in that the bio-organic fertilizer is prepared by the following method steps:
(1) preparing materials: mixing pig manure, cassava residues, banana tree stems and leaves, banana peel, molasses baking powder and earthworm soil;
(2) primary fermentation: uniformly mixing the prepared materials, and carrying out stack retting fermentation for 6-7 d to prepare an organic fertilizer semi-finished product;
(3) inoculating compound bacteria: after the water content of the semi-finished organic fertilizer is adjusted to 40% -50%, adding compound bacterial liquid;
(4) and (3) secondary fermentation: and inoculating the compound bacterial liquid to the semi-finished organic fertilizer, and performing secondary fermentation, wherein the material is turned for 1 time every 2 days in the fermentation process, the material is turned for 3-5 times totally, and the preparation of the biological organic fertilizer can be completed after 7-10 days.
9. The application of the biological organic fertilizer for preventing banana vascular wilt according to any one of claims 1-8 in preventing and controlling banana vascular wilt or promoting banana yield increase.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011531201.6A CN112457125A (en) | 2020-12-22 | 2020-12-22 | Banana wilt prevention bio-organic fertilizer and preparation method and application thereof |
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| CN112899188A (en) * | 2021-01-29 | 2021-06-04 | 西南大学 | Microbial agent for promoting crop root development and preparation and application thereof |
| CN113087571A (en) * | 2021-04-25 | 2021-07-09 | 天津科技大学 | Special microbial fertilizer for strawberries and preparation method thereof |
| CN115226455A (en) * | 2022-07-22 | 2022-10-25 | 海南宝绿春农业开发有限公司 | Fertilizing method for conditioning banana continuous cropping obstacle soil |
| CN116746353A (en) * | 2023-07-10 | 2023-09-15 | 中国热带农业科学院热带作物品种资源研究所 | Method for preventing and controlling banana vascular wilt by fertilizer adjustment |
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