CN107176855B - Bio-organic fertilizer and application thereof - Google Patents
Bio-organic fertilizer and application thereof Download PDFInfo
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- CN107176855B CN107176855B CN201610135194.5A CN201610135194A CN107176855B CN 107176855 B CN107176855 B CN 107176855B CN 201610135194 A CN201610135194 A CN 201610135194A CN 107176855 B CN107176855 B CN 107176855B
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 45
- 239000002689 soil Substances 0.000 claims abstract description 65
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 10
- 241000534630 Brevibacillus choshinensis Species 0.000 claims abstract description 10
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 7
- 210000003608 fece Anatomy 0.000 claims abstract description 7
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 235000013311 vegetables Nutrition 0.000 claims abstract 2
- 241000234314 Zingiber Species 0.000 claims description 37
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 14
- 239000011591 potassium Substances 0.000 claims description 14
- 229910052700 potassium Inorganic materials 0.000 claims description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 241000194108 Bacillus licheniformis Species 0.000 claims description 9
- 239000005416 organic matter Substances 0.000 claims description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052698 phosphorus Inorganic materials 0.000 claims description 7
- 239000011574 phosphorus Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 3
- 239000004021 humic acid Substances 0.000 claims description 3
- 235000019484 Rapeseed oil Nutrition 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 17
- 238000002360 preparation method Methods 0.000 abstract description 10
- 235000004977 Brassica sinapistrum Nutrition 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 244000188595 Brassica sinapistrum Species 0.000 abstract 1
- 238000009264 composting Methods 0.000 abstract 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 36
- 235000008397 ginger Nutrition 0.000 description 36
- 230000000694 effects Effects 0.000 description 8
- 230000004720 fertilization Effects 0.000 description 8
- 244000005700 microbiome Species 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 241000186361 Actinobacteria <class> Species 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 4
- 235000015278 beef Nutrition 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 240000002791 Brassica napus Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 235000021049 nutrient content Nutrition 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000011265 semifinished product Substances 0.000 description 3
- 244000000000 soil microbiome Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 235000019691 monocalcium phosphate Nutrition 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000021232 nutrient availability Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a biological organic fertilizer, belongs to the technical field of agricultural soil biological fertilizers, and particularly relates to preparation and application of a biological organic fertilizer. The biological organic fertilizer is prepared by the following method: mixing rabbit dung, mushroom dregs and rape seed cake in the dry matter weight ratio of 6 to 3 to 1, composting and fermenting to obtain semi-finished organic fertilizer, and inoculating Bacillus licheniformisBacillus licheniformis) Bacillus amyloliquefaciens (A) and (B)B.amyloliquefaciens) And Brevibacillus choshinensis: (Brevibacillus choshinensis) And mixing and then carrying out secondary fermentation. The invention takes rabbit dung, mushroom dregs and vegetable seed cakes as main raw materials, and the high-quality organic fertilizer is prepared by adding functional bacteria and performing rapid fermentation.
Description
Technical Field
The invention belongs to the technical field of agricultural soil biofertilizers, and particularly relates to preparation and application of a bio-organic fertilizer.
Background
With the rapid development of agriculture in China, the large application of chemical fertilizers not only causes the reduction of the organic matter content of soil, but also causes the problems of soil hardening, serious acidification and the like, and influences the survival and propagation of soil microorganisms, thereby causing the reduction of crop yield and quality. The biological organic fertilizer is a specific product containing living microorganisms and having a fertilizer effect, and is a novel fertilizer which can activate soil and does not pollute the environment. The biological organic fertilizer integrates the advantages of organic fertilizers, inorganic fertilizers and microbial fertilizers, not only can effectively supplement organic nutrients of soil, but also can improve the water and fertilizer retention performance of the soil, and has important effects on repairing the soil, maintaining the ecological balance of the soil and promoting the growth of crops. The organic fertilizer in the prior art is mainly used for improving the utilization efficiency of crop nutrient components, promoting the growth and development of crops, improving soil properties, degrading toxic and harmful substances in soil and the like, but the products have the problems of uneven quality, unstable action effect, weak pertinence of strains in the products, poor regional ecological adaptability and the like.
Disclosure of Invention
The invention aims to provide preparation and application of a biological organic fertilizer, and the organic fertilizer is low in cost, green and environment-friendly, simple in preparation method, strong in pertinence of functional bacteria and strong in regional ecological adaptability.
The invention provides a bio-organic fertilizer which is prepared by taking rabbit manure, mushroom dregs and rape seed cake as main raw materials and adding functional bacteria for secondary fermentation. The preparation method comprises the following steps:
(1) preparing materials: mixing the rabbit manure, the bacterial slag and the rape seed cake according to the mass ratio of dry substances of 6:3: 1;
(2) primary fermentation: uniformly mixing the prepared materials, and carrying out stack retting fermentation for 6-7 d to prepare an organic fertilizer semi-finished product;
(3) inoculating functional bacteria: after the water content of the semi-finished organic fertilizer is adjusted to 45-55 percent, three functional bacteria seed solutions including bacillus licheniformis (Bacillus licheniformis)Bacillus licheniformis) Bacillus amyloliquefaciens (A) and (B)B. amyloliquefaciens) And Brevibacillus choshinensis: (Brevibacillus choshinensis) Adding the components according to the proportion of 1:0.8-1.2:0.8-1.2 respectively;
(4) and (3) secondary fermentation: and (3) inoculating functional bacteria to the semi-finished product of the organic fertilizer, and then performing secondary fermentation, wherein in the fermentation process, the material is turned for 1 time every 2 days, the material is turned for 3-5 times totally, and the preparation of the biological organic fertilizer can be completed after 7-10 days.
The invention provides a biological organic fertilizer which is characterized in that the water content of the biological organic fertilizer is 25-28%, the pH value is 6.8-7.5, the number of faecal coliform groups is less than 100/g, and the total viable bacteria activity is more than 2.3 × 109cfu/g, 45-48% of organic matter, 12-15% of humic acid, 1.4-1.8% of total nitrogen, 0.6-0.8% of total phosphorus and 1.1-1.3% of total potassium.
The biological organic fertilizer provided by the invention has the main application effects in ginger planting: compared with the conventional fertilization, the yield of ginger per mu is increased by 1704kg (fresh weight), the yield is increased by 40.26 percent, and the occurrence of diseases and insect pests such as ginger blast and the like is effectively inhibited. The quantity of soil bacteria, fungi and actinomycetes is increased to different degrees, and the micro-ecological environment of ginger soil is effectively improved by taking more bacteria. The pH value of the soil is increased to be close to a neutral environment, so that the effectiveness of soil nutrients is improved; the organic matter content of the soil is increased to 67.38-69.11 g/kg, which is beneficial to improving the soil structure and provides a solid material foundation for the life activities of microorganisms; the ginger has high absorption and utilization rate on nitrogen and potassium, so that the alkaline hydrolysis nitrogen and the quick-acting potassium in the soil are reduced to different degrees, and the reduction range of the quick-acting potassium is maximum.
Detailed Description
1. Preparation of biological organic fertilizer
(1) Preparation of organic fertilizer semi-finished product
The rabbit manure, the bacterial residues and the rapeseed cakes are used as main raw materials, mixed according to the mass ratio of 6:3:1 of dry substances, and are piled, retted and fermented for 6-7 days to prepare the semi-finished organic fertilizer.
(2) Preparation of seed liquid of strain
Separating and purifying healthy ginger rhizosphere as target, and screening to obtain bacillus licheniformis (A)Bacillus licheniformis) Bacillus amyloliquefaciens (A) and (B)B. amyloliquefaciens) And Brevibacillus choshinensis: (Brevibacillus choshinensis) Respectively inoculating the activated extract on a culture dish of beef extract peptone at 35oC, culturing in incubator for 24 h, inoculating cultured bacteria into test tube culture medium, and preparing culture medium with volume of 100 m L and 500m LSeveral conical flasks, wherein a 100 m L conical flask is poured with 20 m L beef extract peptone medium, a 500m L conical flask is poured with 237.5m L beef extract peptone medium, subpackaging is carried out, high-temperature sterilization is carried out for later use, the cultured three functional bacteria are inoculated into a 100 m L conical flask filled with 20 m L beef extract peptone medium, and then 35 moC. Culturing for 22-25 h at the shaking table speed of 170-.
(3) Fermentation culture
After the water content of the semi-finished organic fertilizer is adjusted to 45-55 percent, the bacillus licheniformis (B), (B)Bacillus licheniformis) Bacillus amyloliquefaciens (A) and (B)B. amyloliquefaciens) And Brevibacillus choshinensis: (Brevibacillus choshinensis) Adding seed liquid at a ratio of 1:0.8-1.2:0.8-1.2, respectively, performing secondary fermentation, turning over material for 1 time every 2 days in the fermentation process, turning over material for 3-5 times, and completing preparation of bio-organic fertilizer after 7-10 days to make total bacteria activity more than 0.2 × 109cfu/g。
(4) Analysis of characteristics of finished bio-organic fertilizer
Through detection, the prepared bio-organic fertilizer has the water content of 25-28 percent, the pH value of 6.8-7.5, the number of faecal coliform bacteria is less than 100/g, and the total viable bacteria content is more than 2.3 × 109cfu/g, 45-48% of organic matter, 12-15% of humic acid, 1.4-1.8% of total nitrogen, 0.6-0.8% of total phosphorus and 1.1-1.3% of total potassium.
2. Use of bio-organic fertilizer
The ginger is used as a test object, and the basic properties of soil in a test field are shown in table 1. The experiment was set to 3 treatments: t is1Blank control zone, T2Conventional fertilization zone, T3A biological organic fertilizer application area. Each cell area is 10 m2Repeating the steps three times and randomly designing.
TABLE 1 soil basic Properties of test plot (0-20 cm soil horizon)
The fertilizer dosage of the biological organic fertilizer application area is 1500 kg/mu, which is equivalent to nitrogen (N) and phosphorus (P) per mu2O5) Potassium (K)2O) content 21.0, 24.1, 21.8 kg. because the soil pH of the test area is 4.4, the acidity is stronger, the effectiveness of phosphorus is low, 150 kg of calcium superphosphate is applied to each mu of soil during the test, the fertilizer usage amount of the conventional fertilization area is calculated according to the fertilizer usage amount and nutrient content of the bio-organic fertilizer fertilization area to ensure that the nutrient content of the treatment room is the same, the depth of a rotary tillage ginger field is 20cm after the fertilizer base fertilizer is applied, furrowing and ridging are performed, the row spacing is 20cm × 70 cm after the seeding is performed with 28-30 cm. furrow depth, soil covering, watering, weeding and deinsectization are performed, film covering are performed, and other management methods are performed according to the.
Collecting each processed ginger sample and ginger rhizosphere and non-rhizosphere soil samples in a ginger harvesting season, recording the yield, and determining the number of soil microorganisms and the soil nutrient content.
(1) Difference in ginger yield
As can be seen from figure 1, after the bio-organic fertilizer is applied, the yield (fresh weight) of ginger per mu is increased by 1704kg compared with the conventional fertilizer application, and the yield is increased by 40.26%. In the test process, the ginger in the bio-organic fertilizer application area grows vigorously, leaves are thick, stems are thick and strong, symptoms such as yellow leaves and withering rarely occur, no pest and disease damage occurs, and the ginger is good in color and luster and uniform in size. And about 10-20% of ginger seedlings in the conventional fertilizing area have disease and pest symptoms such as ginger blast and the like, yellow leaves and stems are thin, and the sizes of the harvested gingers are uneven. The biological organic fertilizer can promote the increase of the yield of the ginger and can prevent and treat diseases and insect pests such as ginger blast and the like.
(2) Difference in microbial count in soil
As can be seen from figures 2-4, after the bio-organic fertilizer is applied, the soil microorganisms are mainly bacteria, are secondly actinomycetes and fungi, and the number of the soil microorganisms is significantly higher than that of the soil microorganisms applied by the conventional fertilization, wherein the number of rhizosphere soil bacteria and the number of non-rhizosphere soil bacteria of the ginger respectively reach 7.82 × 106、8.80×106cfu/g is 6.26 and 2.32 times of that of conventional fertilizer application, and the number of fungi is 8.40 × 104、1.06×105cfu/g 4.67 and 1.61 times of that of conventional fertilizer application, and actinomycetes 1.32 × 105、7.47×105cfu/g, 1.76, 1.74 times of the conventional fertilization. The biological organic fertilizer can effectively improve the microecological environment of ginger soil and enrich the biological diversity of soil.
(3) Change of physical and chemical properties of soil
As can be seen from figures 5-9, the physicochemical properties of the soil are changed to different degrees after the bio-organic fertilizer is applied. Specifically, the pH value of the soil is improved from acidity (5.36) before planting to 6.13-6.40, and the soil is in a slightly acidic environment close to neutral, which is favorable for the growth of ginger and is an advantage of strong soil nutrient availability. The organic matter content of the soil is obviously improved, the organic matter content of ginger rhizosphere soil and non-rhizosphere soil reaches 67.38 g/kg and 69.11 g/kg, which are 3.52 times and 2.81 times of that of conventional fertilization. The increase of the organic matter content of the soil is not only beneficial to improving the soil structure, but also is a main source of soil nutrient supply, and provides a solid material foundation for the life activities of microorganisms. The alkaline hydrolysis nitrogen and the quick-acting potassium of the soil are reduced to different degrees, and the reduction range of the quick-acting potassium is the largest, which is related to the absorption and utilization of available nutrients. After the biological organic fertilizer is applied, although the content of alkaline hydrolysis nitrogen is reduced compared with that of the conventional fertilizer, nitrogen can be continuously released for crops to absorb and utilize due to the high content of organic matters in the soil, and the yield of the ginger is improved. The potassium content affects the quality of ginger, and a high yield requires a high supply of potassium. Due to the strong mobility of potassium ions, after the bio-organic fertilizer is applied, the deficiency of potassium content is serious, which indicates that the utilization efficiency of the potassium in soil by the ginger is higher than that of conventional fertilization. The available phosphorus content in the soil does not vary significantly.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 shows the economic yield and the difference between the yield of the stem and leaves of ginger processed by each treatment.
FIG. 2 shows the difference in the number of bacteria in rhizosphere soil and non-rhizosphere soil of ginger treated in each treatment.
FIG. 3 shows the difference in the amount of rhizosphere soil and non-rhizosphere soil fungi of each treated ginger.
FIG. 4 shows the difference in the number of actinomycetes in rhizosphere soil and non-rhizosphere soil of each treated ginger.
FIG. 5 shows the pH value changes of rhizosphere soil and non-rhizosphere soil of ginger in each treatment.
FIG. 6 shows the change of organic matter content in rhizosphere soil and non-rhizosphere soil of ginger processed in each treatment.
FIG. 7 shows the variation of alkaline hydrolysis nitrogen content in rhizosphere soil and non-rhizosphere soil of each treated ginger.
FIG. 8 shows the change of available phosphorus content in rhizosphere soil and non-rhizosphere soil of ginger in each treatment.
FIG. 9 shows the variation of the available potassium content in rhizosphere soil and non-rhizosphere soil of ginger in each treatment.
Claims (3)
1. A bio-organic fertilizer is characterized in that rabbit manure, mushroom dregs and rapeseed oil are used as raw materials, bacillus licheniformis, bacillus amyloliquefaciens and brevibacillus choshinensis are added, and secondary fermentation is carried out to prepare the bio-organic fertilizer, wherein the water content of the bio-organic fertilizer is 25% -28%, the pH value is 6.8-7.5, the number of faecal coliform groups is less than 100/g, and the total viable bacteria content is more than 2.3 × 109cfu/g, 45-48% of organic matter, 12-15% of humic acid, 1.4-1.8% of total nitrogen, 0.6-0.8% of total phosphorus and 1.1-1.3% of total potassium.
2. The bio-organic fertilizer according to claim 1, characterized in that: the mass ratio of the rabbit manure to the mushroom dregs to the dried vegetable cake is 6:3: 1.
3. The bio-organic fertilizer according to claim 2, characterized in that: the bacillus licheniformis, the bacillus amyloliquefaciens and the brevibacillus choshinensis are obtained by separating and identifying healthy ginger plants and rhizosphere soil, are subjected to fermentation culture after being activated, and are mixed with raw materials for fermentation according to the proportion of 1:0.8-1.2: 0.8-1.2.
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| CN108849985B (en) * | 2018-05-30 | 2021-02-26 | 唐山市农业科学研究院 | Ternary compound biological agent and application thereof in prevention and treatment of ginger bacterial wilt |
| CN108947601A (en) * | 2018-09-20 | 2018-12-07 | 徐兆林 | A kind of technical method for allowing cake-shaped rape seed dregs Rapid Fermentation |
| CN110367284A (en) * | 2019-08-07 | 2019-10-25 | 金华市农业科学研究院 | A kind of biological pesticide composite and preparation method thereof and the application in prevention and treatment ginger bacterial wilt of ginger |
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| CN102154175A (en) * | 2011-01-25 | 2011-08-17 | 周清明 | Brevibacillus choshinensis X23 and application thereof |
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| CN102154175A (en) * | 2011-01-25 | 2011-08-17 | 周清明 | Brevibacillus choshinensis X23 and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| Soil solarization and amelioration with calcium chloride or Bacillus licheniformis - an effective integrated strategy for the management of bacterial wilt of ginger incited by Ralstonia pseudosolanacearum;R. Suseela Bhai等;《Eur J Plant Pathol》;20190410;第903-917页 * |
| 地衣芽抱杆菌在种植业上应用研究进展;刘付军;《中国园艺文摘》;20131231(第8期);第55页下转第64页 * |
| 微生物菌剂对生姜土壤养分和菌落结构的影响;王西祥;《中国优秀硕士学位论文全文数据库 农业科技辑》;20150115;D048-25 * |
| 生物有机肥的发酵工艺及应用效果研究;扎史品楚等;《环境工程》;20151231;第33卷;第1011-1014页下转第1020页 * |
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