CN110627537A - Multifunctional microbial fertilizer and preparation method thereof - Google Patents
Multifunctional microbial fertilizer and preparation method thereof Download PDFInfo
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- CN110627537A CN110627537A CN201810643778.2A CN201810643778A CN110627537A CN 110627537 A CN110627537 A CN 110627537A CN 201810643778 A CN201810643778 A CN 201810643778A CN 110627537 A CN110627537 A CN 110627537A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a multifunctional microbial fertilizer and a preparation method thereof. The technical scheme of the invention is that pig manure, seaweed and mushroom residues are mixed according to the mass ratio of 40:20:40, inoculated into the tobacco arthrobacter fermentation broth in an inoculation amount of 10 percent and fermented in a solar aeration aerobic fermentation tank for 15-20 days to form a primary fermentation material; uniformly mixing the bacillus mucilaginosus fermentation liquor and the bacillus amyloliquefaciens fermentation liquor with the primary fermentation material according to the inoculation amount of 5%, and fermenting for 5-7 days to finally obtain the required multifunctional microbial fertilizer. The multifunctional microbial fertilizer can promote the development of crop roots, promote the growth of crops, improve the disease resistance of crops, well inhibit tobacco bacterial wilt and improve the quality and yield of crops to a certain extent.
Description
Technical Field
The invention relates to a fertilizer, in particular to a multifunctional microbial fertilizer.
Background
Along with the improvement of urbanization level, the demand of people for meat and eggs is continuously improved, the number of livestock and poultry farms is increased, and the scale is enlarged. Pig manure is brought to intensive pig raising, and untreated pig manure seriously pollutes the ecological environment of a farmland, causes eutrophication of surface water, brings odor and the like. Domestic excrementThe treatment technology mainly comprises a compost fermentation method, a methane fermentation method and the like[1]。
The edible fungus industry plays a certain role in agricultural development. According to statistics, one ton of edible fungi produced in an industrial production process averagely produce four tons of mushroom residues, and a large amount of mushroom residues are combusted or randomly discharged, so that serious environmental pollution of atmosphere and water is caused. The mushroom residue can be used as edible mushroom reproduction ingredient, livestock ingredient, etc. The mushroom dregs contain various mycoprotein, metabolites and unutilized nutrient substances, have higher heat energy compared with other agricultural wastes, and are better compost raw materials[2]。
The use of seaweeds in agriculture has a long history. The seaweed is rich in various inorganic and organic substances useful for crop growth, and the effective components of the seaweed are extracted by chemical, physical or biological methods to prepare fertilizer for plant to use as nutrient. The seaweed fertilizer contains seaweed polysaccharide, auxin, gibberellin and the like, can stimulate the production of nonspecific active factors in plants and regulate the balance of endogenous hormones, can promote the growth of the plants, improve the yield and improve the quality of agricultural products[3]。
CN201510769449.9 discloses a method for growing a bio-organic fertilizer by using pig manure and mushroom dregs, which comprises the steps of selecting bacillus subtilis to thoroughly decompose the pig manure and the mushroom dregs, and then compounding probiotics with antagonistic or inhibitory action on harmful fungi.
CN201410514541.6 discloses a process for preparing a pig manure bio-organic fertilizer by a microbial fermentation method, wherein 10% -20% of seaweed is added in compost ingredients, so that the prepared bio-organic fertilizer has the advantages of seaweed fertilizers, but the preparation process is complex and is inconvenient for actual operation of agricultural production.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a process method of a multifunctional microbial fertilizer, which takes pig manure, seaweed and mushroom residues as main raw materials, inoculates tobacco arthrobacter, carries out primary fermentation, and then adds bacillus mucilaginosus and bacillus amyloliquefaciens to continue fermentation, thus obtaining the required multifunctional microbial fertilizer.
The multifunctional microbial fertilizer prepared from the arthrobacter tabacum comprises pig manure, seaweed, mushroom residues, arthrobacter tabacum fermentation liquor and bacillus mucilaginosus fermentation liquor. Calculated according to the mass ratio, the pig manure is: seaweed: mushroom residue =40:20: 40. The inoculum sizes of the Arthrobacter tabacum fermentation liquor, the Bacillus mucilaginosus fermentation liquor and the Bacillus amyloliquefaciens are respectively 10%, 5% and 5%.
The raw materials are processed according to the following steps:
A. mixing raw materials: preparing the pig manure, the seaweed and the mushroom residues according to the mass ratio of 40:20:40, stacking the prepared pig manure, seaweed and mushroom residues in a solar aeration aerobic fermentation tank, and uniformly mixing;
b: scattering the prepared separately fermented tobacco bacillus fermentation liquor into the mixed material of pig manure, seaweed and mushroom residue in an inoculation amount of 10%, and uniformly mixing;
c: controlling the water content to be about 55-60%, and performing primary fermentation. Keeping a wet state in the fermentation process, regularly observing the change of water content, supplementing water in time, and turning by using a turning machine when the temperature of a stack reaches above 55 ℃, wherein the primary fermentation time is 15-20 days;
d: respectively and uniformly spreading the bacillus mucilaginosus fermentation liquor and the bacillus amyloliquefaciens fermentation liquor which are obtained by independent fermentation into the primary fermentation material according to the inoculation amount of 5 percent, uniformly mixing by using a turner, and performing secondary fermentation for about 5 to 7 days to obtain the required multifunctional microbial fertilizer.
The Arthrobacter nicotianae is Arthrobacter nicotianae (Arthrobacter nicotianae) three-torch-16, which is preserved in China general microbiological culture Collection center (CGMCC) at 27 months 2 and 2018, and the number of the preservation registration is CGMCC No. 15402; the Bacillus mucilaginosus is a Bacillus mucilaginosus (Bacillus mucoarginosus) three-torch-01 strain which is preserved in China general microbiological culture Collection center on 7-month and 2-month 2010, and the number of the preservation registration is CGMCC NO. 3995; the bacillus amyloliquefaciens is bacillus amyloliquefaciens (Bacillus amyloliquefaciens) Sanju-17 which is preserved in China general microbiological culture Collection center (CGMCC) in 2018, 2 and 27 months and has the preservation registration number of CGMCC No. 15403.
The number of effective live bacteria in fermentation liquor of the arthrobacter tabacum, the bacillus mucilaginosus and the bacillus amyloliquefaciens is more than 2 hundred million/mL.
The content of organic matters in the multifunctional microbial fertilizer is more than or equal to 45 percent, the number of effective live bacteria is more than or equal to 10 hundred million/g, the number of effective live bacteria of the arthrobacter tabacum is more than or equal to 8 hundred million/g, the number of effective live bacteria of the bacillus mucilaginosus is more than or equal to 2 hundred million/g, and the number of effective live bacteria of the bacillus amyloliquefaciens is more than or equal to 2 hundred million/.
The invention has the following beneficial effects:
1. the invention has obvious advantages in the aspect of quickly decomposing the pig manure and the mushroom residues, the solar aeration aerobic fermentation tank has a flora structure, dissolved oxygen and constant temperature, and the decomposition speed is accelerated by adding the arthrobacter tabacum, so that the problem of instability of the degradation products of the traditional organic fertilizer is further solved.
2. The Bacillus mucilaginosus (Bacillus licheniformis) triple-torch-01 strain used by the invention has high yield of natural plant hormone and free amino acid, and the yield is more than 190 ppm and 300 ppm in 2 d; the natural phytohormone in the bacillus mucilaginosus fermentation liquor exceeds 120 ppm, the free amino acid is as high as 400 ppm, and the bacillus mucilaginosus fermentation liquor has strong plant growth promoting capability.
3. After the multifunctional microbial fertilizer is applied, the development of crop root systems can be promoted, the growth of crops can be promoted, the disease resistance of crops can be improved, and particularly, the multifunctional microbial fertilizer has a relatively obvious inhibiting effect on tobacco bacterial wilt.
4. The multifunctional microbial fertilizer has the advantages of simple process, low cost, safety, environmental protection and high popularization value.
Detailed Description
The present invention will be described in more detail below with reference to examples for further understanding, but the present invention is not limited to the following examples.
EXAMPLE 1 preparation of multifunctional microbial Fertilizer
The pig manure is prepared from the following raw materials in parts by mass: mixing seaweed and mushroom residue =40:20:40, wherein the inoculation amounts of the tobacco arthrobacter fermentation liquid, the jelly-like bacillus fermentation liquid and the amyloliquefaciens fermentation liquid are respectively 10%, 5% and 5%. The raw materials are processed according to the following steps:
A. mixing raw materials: preparing the pig manure, the seaweed and the mushroom residues according to the mass ratio of 40:20:40, and uniformly mixing in a solar aeration aerobic fermentation tank;
b: spreading the prepared tobacco bacillus fermentation liquid fermented by the single liquid into pig manure and mushroom residues according to the inoculation amount of 10%, and uniformly mixing;
c: controlling the water content to be about 60%, and performing primary fermentation;
d: when the temperature of the stack reaches above 55 ℃, turning and throwing by using a turner, wherein the primary fermentation time is 15-20 days;
e: respectively and uniformly spreading the separately fermented bacillus mucilaginosus and bacillus amyloliquefaciens fermentation liquid into the primary fermentation material according to the inoculation amount of 5 percent, uniformly mixing by using a turner, and performing secondary fermentation for 5 days to obtain the multifunctional microbial fertilizer.
The preparation method of the Arthrobacter nicotianae fermentation liquid comprises the following steps:
preparing a slant culture medium, inoculating the arthrobacter tabacum to the slant culture medium for streak culture, picking a ring of activated arthrobacter tabacum from the slant, inoculating the ring of activated arthrobacter tabacum to a liquid seed culture medium to obtain a seed solution, and inoculating the seed solution to a fermentation culture medium to obtain the required arthrobacter tabacum fermentation broth.
The slant culture medium comprises the following components: tryptone 1 g/L, yeast powder 0.5 g/L, sodium chloride 1 g/L, agar powder 20 g/L, pH7.0. The slant culture condition is culture at 28 ℃ for 36 h; the liquid seed culture medium comprises tryptone 1 g/L, yeast powder 0.5 g/L, sodium chloride 1 g/L, and pH7.0. The seed culture condition is that the seed is cultured for 24 hours at the temperature of 28 ℃ and at the speed of 200 r/min. The fermentation medium can be composed of tryptone 0.5 g/L, potassium dihydrogen phosphate 1.5 g/L, magnesium sulfate heptahydrate 1.25 g/L, calcium chloride 0.05 g/L, zinc sulfate heptahydrate 0.02 g/L, ferrous sulfate heptahydrate 0.01 g/L, and pH7.0. The fermentation culture condition is that the culture is carried out for 48 hours at the temperature of 28 ℃ and at the speed of 200 r/min.
The preparation method of the bacillus mucilaginosus fermentation liquor comprises the following steps:
preparing nitrogen-free solid culture medium, and culturing Bacillus mucilaginosus (B.), (Bacillus Mucilaginosus) Inoculating the strain of the third torch 01 to a nitrogen-free solid culture medium for streak culture, and activating the bacillus mucilaginosus on the nitrogen-free solid culture medium (the first strain is bacillus mucilaginosus and the second strain is bacillus mucilaginosus)Bacillus Mucilaginosus) The three-torch 01 strain is transferred to a nitrogen-free liquid culture medium, a primary seed solution is obtained through culture, the primary seed solution is inoculated to a seed fermentation tank, and a secondary seed solution is obtained through culture. Inoculating the seed liquid into a fermentation culture medium for fermentation to prepare a bacillus mucilaginosus fermentation liquid;
the solid nitrogen-free culture medium comprises 10 g/L of sucrose, 0.8 g/L of dipotassium phosphate, 0.4 g/L of magnesium sulfate, 0.15 g/L of sodium chloride, 0.5 g/L of calcium carbonate and 20 g/L of agar. The pH value before sterilization is 7.5; the activation culture condition is to culture for 48 hours at the temperature of 30 ℃; the seed culture medium comprises 10 g/L of sucrose, 0.8 g/L of dipotassium phosphate, 0.4 g/L of magnesium sulfate, 0.15 g/L of sodium chloride and 0.5 g/L of calcium carbonate, and the pH value is 7.5 before sterilization. The condition of the first-stage seed culture can be at 30 ℃ and 150 rpm for 24 h; the secondary seed culture condition can be in a seed fermentation tank, the rotation speed of the tank is kept at 125-150 rpm under the condition of 30 ℃, and the culture is carried out for 24 hours; the fermentation medium comprises 1.5 g/L of yeast extract, 5.0 g/L of starch, 5.0 g/L of soybean meal, 1.2 g/L of magnesium sulfate, 2 g/L of dipotassium phosphate, 8.5 g/L of calcium carbonate and 0.2 g/L of sodium chloride, and the pH value is 7.2 before sterilization; the inoculation amount of the seed liquid inoculated into the culture medium of the fermentation tank for fermentation is 10 percent of the seed liquid according to the volume percentage; the fermentation conditions may be: the rotation speed is controlled at 200 rpm, the dissolved oxygen is controlled at 20% (V/V) by adjusting the rotation speed and the ventilation quantity, the tank temperature is controlled at 30 ℃, the tank pressure is kept at 0.05-0.06 MPa, the pH value is controlled at 7.2 by 10% of ammonia water and 10% of hydrochloric acid, and the fermentation time is 60 hours.
The preparation method of the bacillus amyloliquefaciens comprises the following steps:
preparing a slant culture medium, inoculating the bacillus amyloliquefaciens to the slant culture medium, and culturing for 36 hours at the temperature of 30 ℃. Inoculating the activated bacillus amyloliquefaciens into a seed culture medium, and carrying out shaking culture at 30 ℃ and 150 r/min for 24 h. Then inoculating the seed liquid into a fermentation culture medium according to the inoculation amount of 6%, and culturing for 48 h under the conditions of 28 ℃ and 150 r/min.
The composition of the slant culture medium can be 3 g/L of beef extract, 10 g/L of peptone, 2.5 g/L of glucose, 15 g/L of agar and pH 7.2-7.4; the seed culture medium comprises beef extract 3 g/L, peptone 10 g/L, glucose 2.5 g/L, and pH7.2-7.4. The fermentation medium comprises 16 g/L of soybean meal, 6.5 g/L of corn starch, 0.8 g/L of magnesium sulfate heptahydrate, 1.25 g/L of sodium chloride and 0.75 g/L of potassium dihydrogen phosphate.
EXAMPLE 2 Effect of multifunctional microbial Fertilizer on tomato growth
(1) Basic conditions of the test cell:
the test time is 2016, 8, ~ 2017, 1, and the test site is Nanjing county, Zhangzhou, Fujian province.
The test field has flat vigor and uniform land force, and the soil to be tested is sandy loam. Soil samples with the depth of 0-20 cm are collected before the test, and the basic conditions of the soil are measured as follows:
TABLE 1 soil conditions tested
(2) Design of test protocol
The test plant is tomato, the variety is Shenshi 308, the test is provided with 4 treatments and random arrangement, each treatment is repeated for 4 times, and the cell area is about 30 m2The test treatment components are as follows:
treatment 1: blank control
And (3) treatment 2: conventional fertilization
And (3) treatment: multifunctional microbial fertilizer subjected to conventional fertilization and inactivated application
And (4) treatment: multifunctional microbial fertilizer for conventional fertilization and application
The specific fertilization method comprises the following steps: the multifunctional microbial fertilizer is treated in a transplanting period (9 months and 15 days), a fruit setting period (10 months and 9 days) and a harvesting period (11 months and 13 days) for fertilization. Treatment 1: blank control, no fertilization; and (3) treatment 2: conventional fertilization; and (3) treatment: applying the inactivated multifunctional microbial fertilizer at 150 kg/mu by using the inactivation matrix of the conventional fertilization and the test fertilizer; and (4) treatment: conventional fertilization and active multifunctional microbial fertilizer: the usage and the dosage are the same as the multifunctional microbial fertilizer after the inactivation is applied. And respectively registering the yield of each cell from the beginning of harvesting according to the investigation result, and counting the yield. Biological yield statistics were performed at the end of harvest (1 month and 3 days).
(3) The results are shown below:
a) effect of different treatments on tomato biological traits
And after transplanting, testing the tomato plants in the residential quarter, measuring the number of actual plants and harvested plants of the tomatoes under different treatment conditions, calculating the dead seedling rate, detecting the rotten fruit rate and the plant height, and counting the average value of each index. As can be seen from Table 2, treatment 4 had lower rates of death and rotten fruits and relatively higher plant heights than the other three groups, indicating that the application of the multifunctional microbial fertilizer has a promoting effect on the growth of tomatoes and can reduce the rates of death and rotten fruits.
TABLE 2 Effect of different treatments on tomato traits
b) Effect of different treatments on tomato yield
From the yield (table 3), after the multifunctional microbial fertilizer is applied, the yield per mu is 4775.8 kg, the yield is increased by 502.3 kg compared with the conventional fertilizer, the yield is increased by 11.7%, and the yield of the sterilized multifunctional microbial fertilizer is increased by 88.9 kg compared with the conventional fertilizer, and the yield is increased by 2.1%.
TABLE 3 Effect of different treatments on tomato yield
The results of the anova (F test) (tables 4 and 5) show that the difference between the application of the multifunctional microbial fertilizer and the treatment without applying the fertilizer and the conventional fertilizer application is very significant, which indicates that the application of the multifunctional microbial fertilizer can effectively improve the yield of the tomatoes, and the difference between the multifunctional microbial fertilizer after spraying and sterilization and the conventional fertilizer application is not significant. In conclusion, the multifunctional microbial fertilizer has the effect of increasing the yield of the tomatoes.
TABLE 4 analysis of variance of tomato yield
Note: analysis of variance (. sup. sup
TABLE 5 multiple comparison of tomato yields (SSR method)
(4) The field plot test result shows that:
the field test report shows that the multifunctional microbial fertilizer has obvious effect on the tomato yield increase, the applied multifunctional microbial fertilizer is increased by 502.3 kg compared with a control group, the yield is increased by 11.7 percent, and the difference is very obvious. Compared with the conventional fertilization, the yield of the sterilized multifunctional microbial fertilizer is increased by 88.9 kg, the yield is increased by 2.1 percent, and the difference is not obvious. The functional bacteria in the multifunctional microbial fertilizer play a role in increasing the yield in tomato planting.
EXAMPLE 3 Effect of multifunctional microbial Fertilizer on tobacco growth
(2) Basic conditions of the test cell:
the test time is 2 months in 2017 and ~ 2017 months in 2017 and 8 months in 2017, and the test site is Chongqing Fuling Jordan Tokyo.
The tobacco varieties tested were: yunyan 97.
Summary of the test: the soil to be tested is yellow-red soil, the soil fertilizer level is medium, and the basic properties are as follows:
TABLE 1 soil conditions tested
(2) Design of test protocol
The experiment was carried out with 4 treatments, randomly arranged, repeated 3 times, and a cell area of 60 m2And protective rows are arranged around the test bed, and the test treatment components are as follows:
a: blank control (CK 1)
B: conventional fertilizer (CK 2)
C: multifunctional microbial fertilizer (matrix CK 3) for conventional fertilization and inactivation
D: conventional fertilization and multifunctional microbial fertilizer
Ridging for 3 months and 28 days to 4 months and 2 days according to the specification of 120 cm, wherein the height of a ridge body is more than 35 cm. In the ridging process, base fertilizer is applied according to the conventional production requirement. Treatment group D at 50 kg/666.7m on 12 days 4 months in 2017 (1 day before transplantation)2The multifunctional microbial fertilizer is applied into tobacco holes at the application amount of 50 g per plant. And 4, 13 days, transplanting the tobacco matrix. When the new leaves of the tobacco seedlings exceed the ground surface by 3cm, the special fertilizer for the tobacco is applied in holes by 25 kg/666.7m2. At the same time, control group C was given an equivalent amount of inactivated multifunctional microbial fertilizer (substrate). Test group a was not fertilized and test group B was performed according to conventional production. Other methods for fertilizing and pesticide management are consistent during the growth period.
And (3) surveying 3 representative tobacco plants at each point, and measuring the agronomic characters of the tobacco plants treated, such as plant height, effective leaf number, maximum leaf length, maximum leaf width, stem circumference and the like, when the tobacco leaves are taken at 5 points in each cell 7 days after topping.
The tobacco bacterial wilt was investigated on 7/7 and 27/7.
Tobacco yield calculations were performed on days 8, 15.
(3) The results are shown below:
(a) the influence of the multifunctional microbial fertilizer on various agronomic performance indexes of tobacco plants.
As is clear from Table 2, the tobacco plants in treatment group D were 38.03 cm, 20.41 cm and 13.36 cm higher than those in test group A, B, C at agronomic levels such as plant height, stem circumference, pitch, etc., and the gains were 44.5%, 19.8% and 12.1%, respectively. The stem circumference, the maximum leaf length and the leaf width of the treatment group are all increased compared with those of the treatment A, B, C, which indicates that the multifunctional microbial fertilizer applied to the tobacco can obviously promote the growth of tobacco plants.
TABLE 2 influence of different treatments on various agronomic performance indexes of tobacco plants
As can be seen from Table 3, treatment 4 gave the highest yield, on average 666.7m2The yield was 181.56 kg, which was specific for treatment 2 at 666.7m2The yield is increased by 19.33 kg and increased by 11.91 percent. The application of the multifunctional microbial fertilizer can effectively promote the yield increase of tobacco.
TABLE 3 Effect of different treatments on tobacco yield
It can be known from analysis of variance that the difference between treatments is significant, and the difference between repetitions is not significant, and the F test result shows that the F value between treatments is very significant, and further multiple comparisons show that the differences between treatment 4 and treatment 2 and treatment 3 are very significant.
TABLE 4 analysis of variance of the yields of different treatments
TABLE 5 multiple comparison of tobacco yields
(b) Control situation of multifunctional microbial fertilizer on tobacco bacterial wilt
Treatment A, B, C found evidence of bacterial wilt development at 2/6 and increased the area of development of bacterial wilt at 7/6. The number of bacterial wilt-causing plants and the number of disease stages are not increased rapidly until 7 months and 27 days.
The investigation on 7 months and 7 days shows that the bacterial wilt appears in A, B, C cells, but the bacterial wilt appears less in D cells of the treatment group, the incidence rate is 4.5 percent and is less than that of A, B, C cells of the treatment group. 7 months and 27 days, treatment D incidence was reduced by 5.1, 3.5 and 3.3, respectively, compared to treatment A, B, C; the disease indices were reduced by 6.6, 3.81 and 3.67, respectively, compared to treatment A, B, C. Compared with the treatment B, the relative prevention effect reaches 55.6 percent, which shows that the multifunctional microbial fertilizer has better prevention and treatment effect on bacterial wilt.
TABLE 6 Effect of different treatments on bacterial wilt disease
(4) The field plot test result shows that:
(a) the multifunctional microbial fertilizer can promote the growth and development of tobacco plants after field transplantation and improve the yield of tobacco leaves, and the yield is increased by 11.91 percent compared with the conventional fertilizer application; analysis of variance shows that the yield increase by applying the multifunctional microbial fertilizer reaches an extremely significant level.
(b) The incidence of bacterial wilt of tobacco plants applying the multifunctional microbial fertilizer is obviously lower than that of a control tobacco field, so that the growth vigor of the tobacco plants is enhanced, and the economic benefit is increased.
In addition, the various embodiments of the invention can be combined arbitrarily, and the invention should be regarded as the disclosure of the invention as long as the idea of the invention is not violated.
Reference documents:
1. shaohao, Ma Yang Hui, Jiang Xionghui, the current situation of livestock and poultry manure treatment and utilization in China and the prospect [ J ] agricultural research, 2009, 31(2):222 + 224.
2. An application study of the yunzhang mushroom dregs in melon seedling culture [ D ]. Shanghai university of traffic, 2014.
3. Research progress of inducing plant stress resistance by fipronil, heshuya, linkeming, marine oligosaccharide [ J ]. Chinese agricultural science and technology report 2016, 18(4):44-51.
Claims (5)
1. The multifunctional microbial fertilizer has an organic matter content of more than or equal to 45 percent, and is characterized in that: the number of effective live bacteria of the tobacco arthrobacter is more than or equal to 10 hundred million/g, the number of effective live bacteria of the bacillus mucilaginosus is more than or equal to 8 hundred million/g, the number of effective live bacteria of the bacillus mucilaginosus is more than or equal to 2 hundred million/g, and the number of effective live bacteria of the bacillus amyloliquefaciens is more than or equal to 2 hundred million/g; the Arthrobacter nicotianae is three torches-16 of Arthrobacter nicotianae (Arthrobacter nicotianae), three torches-01 of Bacillus mucilaginosus (Bacillus mucilaginosus) and three torches-17 of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are all preserved in the China general microbiological culture Collection center, and the registration numbers of the preservation center are CGMCC No.15402, CGMCC No.3995 and CGMCC No.15403 respectively;
the multifunctional microbial fertilizer comprises the following raw materials in parts by weight: 40 parts of pig manure, 20 parts of seaweed, 40 parts of mushroom residue, 10 parts of tobacco arthrobacter, 5 parts of bacillus mucilaginosus and 5 parts of bacillus amyloliquefaciens.
2. The multifunctional microbial fertilizer as claimed in claim 1, wherein the seaweed may be one or more of Gracilaria verrucosa, Sargassum, Brown algae, etc.
3. The use of the multifunctional microbial fertilizer as claimed in claim 1 or 2 for promoting the quality improvement and yield increase of crops and improving the disease resistance of crops.
4. The use of the multifunctional microbial fertilizer according to claim 3, for the prevention of tobacco bacterial wilt.
5. The preparation method of the multifunctional microbial fertilizer as claimed in claim 1 or 2, which is obtained by processing the following steps:
A. mixing raw materials: preparing the pig manure, the seaweed and the mushroom residues according to the mass ratio of 40:20:40, stacking the prepared pig manure, seaweed and mushroom residues in a solar aeration aerobic fermentation tank, and uniformly mixing;
b: scattering the prepared tobacco bacillus fermentation liquor fermented by the single liquid into the mixed material of the pig manure, the seaweed and the mushroom residue in an inoculation amount of 5%, and uniformly mixing;
c: controlling the water content to be about 55-60%, performing primary fermentation, keeping a wet state in the fermentation process, regularly observing the change of the water content, supplementing water in time, and turning by using a turner when the temperature of a stack reaches above 55 ℃, wherein the primary fermentation time is 15-20 days;
d: respectively and uniformly spreading the bacillus mucilaginosus fermentation liquor and the bacillus amyloliquefaciens fermentation liquor which are obtained by independent fermentation into the primary fermentation material according to the inoculation amount of 5 percent, uniformly mixing by using a turner, and performing secondary fermentation for about 5 to 7 days to obtain the required multifunctional microbial fertilizer.
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| CN112795518A (en) * | 2021-02-25 | 2021-05-14 | 福建三炬生物科技股份有限公司 | Disease-resistant and quality-improving microbial agent special for tobacco and application thereof |
| CN112919982A (en) * | 2021-02-25 | 2021-06-08 | 福建三炬生物科技股份有限公司 | Compound microbial agent and fertilizer special for eucalyptus |
| CN113213986A (en) * | 2020-01-21 | 2021-08-06 | 吉林农业大学 | Biological organic fertilizer containing bacillus amyloliquefaciens and preparation method and application thereof |
| CN113277910A (en) * | 2021-07-06 | 2021-08-20 | 石河子大学 | Bio-organic fertilizer and preparation method and application thereof |
| CN114149808A (en) * | 2021-11-26 | 2022-03-08 | 云南省烟草公司保山市公司 | Soil conditioner for preventing and treating tobacco bacterial wilt and improving proportion of middle-grade and high-grade tobacco leaves and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110683875A (en) * | 2019-11-13 | 2020-01-14 | 贵州中烟工业有限责任公司 | Biological bacterial fertilizer for preventing bacterial wilt of flue-cured tobacco, preparation method and application |
| CN113213986A (en) * | 2020-01-21 | 2021-08-06 | 吉林农业大学 | Biological organic fertilizer containing bacillus amyloliquefaciens and preparation method and application thereof |
| CN112795518A (en) * | 2021-02-25 | 2021-05-14 | 福建三炬生物科技股份有限公司 | Disease-resistant and quality-improving microbial agent special for tobacco and application thereof |
| CN112919982A (en) * | 2021-02-25 | 2021-06-08 | 福建三炬生物科技股份有限公司 | Compound microbial agent and fertilizer special for eucalyptus |
| CN113277910A (en) * | 2021-07-06 | 2021-08-20 | 石河子大学 | Bio-organic fertilizer and preparation method and application thereof |
| CN114149808A (en) * | 2021-11-26 | 2022-03-08 | 云南省烟草公司保山市公司 | Soil conditioner for preventing and treating tobacco bacterial wilt and improving proportion of middle-grade and high-grade tobacco leaves and preparation method thereof |
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Application publication date: 20191231 |