CN108503466A - A kind of tea tree special bio bacterial manure and the preparation method and application thereof - Google Patents
A kind of tea tree special bio bacterial manure and the preparation method and application thereof Download PDFInfo
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- CN108503466A CN108503466A CN201810692789.XA CN201810692789A CN108503466A CN 108503466 A CN108503466 A CN 108503466A CN 201810692789 A CN201810692789 A CN 201810692789A CN 108503466 A CN108503466 A CN 108503466A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention discloses a kind of tea tree special bio bacterial manure and the preparation method and application thereof,, with 10 parts of Bacillus cercus microbial inoculum, 5 parts of bacillus subtilis microbial agent, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 6 parts of humic acid, 5 parts of bentonite for raw material, tea tree special bio bacterial manure is made in agitated mixing, screening, drying, packaging for it.Enhance tea tree disease resistance applied to the yield, improvement tea tree quality, induction for increasing tea tree, improve tea root micro organism quantity and soil enzyme activities etc..Have many advantages, such as high significant effect, green non-pollution, utilization rate of fertilizer, improvement soil, prevention and control disease, improve yield and quality, and is of low cost, easy to spread.
Description
Technical field
The present invention relates to tree plant cultivation technologies and fertilising field, and in particular to a kind of tea tree special bio bacterial manure and its preparation
Method and application.
Background technology
The poor efficiency of chemical fertilizer and its high environmental risk brought are always to perplex the significant problem in the whole world in agricultural production,
Chemical fertilizer use has also resulted in tea place ecological environment severe exacerbation in Tea Production, and tea leaf quality is difficult to ensure.How to make
The special fertilizer of standby tealeaves green ecological, optimizes growth of tea plant environment, improves the yield and quality of tea tree, to promoting entire tea place to give birth to
The improvement of state system is that tea industry sustainable development is of great significance.And the tea leaf quality of simple application of organic fertilizers production is good,
But yield is not high.Although being increased substantially using the tealeaves yield of inorganic fertilizer production merely, quality is declined.And due to tea
Tree is more batches of harvestings in 1 year, relatively continuous and steady to the absorption of nutrient, without obviously peak period.Therefore tea tree
The fertilizer of application should fertilizer efficiency it is long, in order to avoid frequently fertilising cause root system to injure, nutrient supplies stabilization again, to ensure that blade is equal
It is even, improve tea quality.And bio-bacterial manure is the important side of a kind of environmental friendly fertilizer and the future of agriculture sustainable development
One of to, have and maintain and increase soil fertility, be conducive to the breeding of microorganism sociales, keep ecological balance, reduces ring
Many advantages, such as border is polluted.
Currently, tea tree application fertilizer be mainly Common compound fertilizer and common organic fertilizer, rarely have in the prior art exclusively for
The ideal biological bacterial manure of tea tree design.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, utilization rate of fertilizer be high, improvement
Soil, the tea tree special bio bacterial manure for meeting growth of tea plant nutritional need.
Technical scheme is as follows：
A kind of tea tree special bio bacterial manure, it is made from the following raw materials in parts by weight：8-12 parts of Bacillus cercus microbial inoculum,
3-7 parts of bacillus subtilis microbial agent, 3-7 parts of trichoderma bacteria agent, 25-35 parts of organic fertilizer, 10-15 parts of nitrogenous fertilizer, 6-10 parts of phosphate fertilizer, potassium
10-15 parts fertile, micro- 3-7 parts of fertilizer, 1-3 parts of plant growth-promoting substance, 4-8 parts of humic acid, 3-7 parts of bentonite.
Preferably, it is made from the following raw materials in parts by weight：10 parts of Bacillus cercus microbial inoculum, bacillus subtilis microbial agent
5 parts, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, plant growth-promoting substance 2
Part, 6 parts of humic acid, 5 parts of bentonite.
Wherein, the strain contained in the Bacillus cercus microbial inoculum that the present invention uses can select Bacillus cercus LY05
(CCTCC NO:M 2015397), Bacillus cercus LGY06 (CCTCC NO:M 2015398) and Bacillus cercus WL08
(CCTCCNO:M2015399 a combination of one or more)；Bacillus subtilis and Trichoderma are commercial product.They
Production method be：After bacterial strain expansion connects cultivation and fermentation, bacterium solution is adsorbed with sterile solid absorbent, is placed on after mixing well black
Dark place stands 2 days, is then dried under the conditions of 25-30 DEG C, water tariff collection is within 30% to get solid fungicide, effective viable bacteria
Number >=10,000,000,000/gram.
Further, the solid absorbent is 3 by weight by maize straw powder+peanut shell powder+sawdust:2:1
It is made.
Further, organic fertilizer is by farm animal excrement, stalk, rubsen cake, peat soil, vinasse, Chinese medicine slag, edible fungi
Slag is 5 in mass ratio:2:1:1:0.5:0.5:0.5 uniformly mixing, and water tariff collection is added in 30-35%, pH 6.0-6.8
Enter the EM fermenting agents that mass percent is 2-3%, decomposed post-processing in 15-20 days is made.Nitrogenous fertilizer refers to ammonium fertilizer, nitrate fertilizer
Or the combination of one or more of amide nitrogen fertilizer；The phosphate fertilizer refers to phosphorus pentoxide, natural phosphate fertilizer or chemical phosphorus
The combination of one or more of fertilizer；Potash fertilizer refers to one or both of potassium chloride, potassium sulfate, plant ash, kainite
Above combination；Micro- fertilizer for mass ratio is:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Calcareous fertilisers+boron fertilizer+magnesium of 0.5-1
The mixed fertilizer of fertilizer+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer；Plant growth-promoting substance is that mass ratio is:1-2:1-2:0.1-0.2's
Chitosan+amino acid+seaweeds；Humic acid water content is 3-5%, the content of organic matter >=75%.
The preparation method of the tea tree special bio bacterial manure of the present invention, comprises the technical steps that：
Step 1. takes Bacillus cercus microbial inoculum, bacillus subtilis microbial agent, trichoderma bacteria agent, organic by regulation proportioning
Fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare；
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly；
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, the special life of tea tree is made in screened, drying, packaging
Object bacterial manure.
The bio-bacterial manure of the present invention can also be applied at least one of following (1)-(3)：
(1) yield for increasing tea tree, improves tea tree quality；
(2) induction enhancing tea tree disease resistance, the diseases such as effective prevention and control tea tree root rot, root knot, root-knot nematode；
(3) growth for promoting low age and the tea tree plant that grows into forest, improves tea root micro organism quantity and soil enzyme activities.
Compared with prior art, the present invention bio-bacterial manure of the present invention is using Bacillus cercus microbial inoculum, bacillus subtilis
Microbial inoculum, trichoderma bacteria agent, organic fertilizer, plant growth-promoting substance, a great number of elements and trace element etc. form bio-bacterial manure, can effectively carry
High tea tree economic benefit, improving quality promote plant well growth and improvement root soil environment, and effectively tea tree can be induced to plant
Strain, the enhancing of tea reality disease resistance and the generation for preventing the great root diseases such as tea tree root rot, root knot, root-knot nematode, have effect
Tea is significantly, green non-pollution, utilization rate of fertilizer are high, improvement soil, prevention and control disease pest, improves the advantages that yield and quality, and at low cost
It is honest and clean, easy to spread.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure is formed and is prepared：
Bio-bacterial manure A formula compositions and preparation, (1) composition：10 parts of Bacillus cercus microbial inoculum, bacillus subtilis microbial agent
5 parts, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, plant growth-promoting substance 2
Part, 6 parts of humic acid, 5 parts of bentonite.(2) it prepares：1. taking Bacillus cercus microbial inoculum, bacillus subtilis bacterium by regulation proportioning
Agent, trichoderma bacteria agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare；2. walking
The rapid 1. above raw material is placed in blender is thoroughly mixed uniformly；3. in 25-30 DEG C of temperature, the condition of pH 6.0-6.8
Under, tea tree special bio bacterial manure A is made in screened, drying, packaging.
Bio-bacterial manure B formula compositions and preparation, (1) composition：10 parts of bacillus subtilis microbial agent, 10 parts of trichoderma bacteria agent,
30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 6 parts of humic acid, bentonite
5 parts.(2) it prepares：1. by regulation proportioning take bacillus subtilis microbial agent, trichoderma bacteria agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer,
Micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare；2. step 1. the above raw material is placed in blender be sufficiently stirred it is mixed
It closes uniform；3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, tea tree special bio bacterium is made in screened, drying, packaging
Fertile B.
2. field trial：
Experimental field overview：Experimental field it is located at Guizhou Zhengan County tea tree garden, kind is " Zhengan County's white tea ", and mean sea level is
1276m, subtropical zone monsoon moist climate, has a moderate climate, and 15.2 DEG C average, abundant rainfall, long annual rainfall is in 1100-1300
Between millimeter, the hydro-thermal same period.It is yellow earth for examination tea garden soil type, random in tea place before experiment, multiple spot mixing acquisition depth 0-
The soil sample of 60cm is as soil background, pH value 5.86, total nitrogen content 1.72gkg-1, the content of organic matter is
36.58g·kg-1, alkaline hydrolysis nitrogen content is 106.76mgkg-1, available phosphorus content 4.67mgkg-1, effective potassium content is
3.66mg·kg-1, effective iron content 55.36mgkg-1。
Field experimental design：By the application of tea tree of tea tree special bio bacterial manure, tea garden soil type is yellow earth.Tea place makes
Used time, base manure and top dressing are respectively applied once, and base manure is applied mid-November current year, account for total fertilizer 60%, top dressing is mid-March next year
It applies, accounts for the 40% of fertilizer total amount.As a contrast with conventional organic and inorganic fertilizer, the same bio-bacterial manure of dose.Experiment is set 3 times
It repeats.
3. analysis project and method：
Yield and quality：Tea polyphenols contains in investigation dry tea yield, the young sprout fresh leaf water extraction content of tealeaves, fresh tea leaves
Free aminoacid content, fresh tea leaves Determination of Chlorophyll total amount in amount, fresh tea leaves.Investigate breeding time tea tree root rot, moire blight,
False smaller green leaf hopper incidence counts cumulative life-incidence or insect population number；Incidence (%)=100 × (morbidity strain number/investigation strain
Number).
Soil root micro organism quantity and enzyme assay：Urease activity indophenols colorimetric method for determining, active unit is with 1g
Soil sample hydrolyzes the milligram number of the ammonia nitrogen of generation to indicate under the conditions of 37 DEG C after reaction in 24 hours；Sucrose activity phosphorus
Molybdic acid colorimetric method for determining, unit of enzyme activity is with (37 DEG C, 24 hours) meters of reduced sugar mg/g dry ground；Catalase is with using permanganic acid
Potassium titration, the ml that unit of enzyme activity reacts post consumption 0.1mol/LKMnO4 solution with 1g soil samples in 25 DEG C, 20min are come
It indicates；Alkaline phosphatase disodium phenyl phosphate colorimetric method for determining, unit of enzyme activity is with the 24 hours phenol (PhOH) generated of every gram of soil
Milligram number indicates.Edaphon clump count is measured using colony counting method：Bacterium beef-protein medium culture 24
It is counted after hour；Fungi is counted with PDA culture medium culture；Actinomyces are counted using Gause I synthetic media culture.
4. embodiment effect：
It is extremely aobvious from table 1 to table 3 as can be seen that can effectively increase tea cured leaf yield using tea tree special bio bacterial manure
It writes and improves fresh leaf water extraction, tea polyphenols, free amino acid and chlorophyll content, promote the formation of tea bush productivity and changing for quality
It is kind；And it can effectively reduce the generation of the diseases such as tea tree root rot, root knot, root-knot nematode；And soil enzyme activities can be improved and carried
High soil microbe quantity.
Influence of the application bio-bacterial manure of table 1 to tea bush productivity quality
Note：With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
To disease for tea plant, a situation arises for the application of table 2 bio-bacterial manure
Influence of the application bio-bacterial manure of table 3 to tea soil enzymatic activity and micro organism quantity
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.
Claims (6)
1. a kind of tea tree special bio bacterial manure, it is characterised in that：It is made from the following raw materials in parts by weight：Bacillus cercus bacterium
8-12 parts of agent, 3-7 parts of bacillus subtilis microbial agent, 3-7 parts of trichoderma bacteria agent, 25-35 parts of organic fertilizer, 10-15 parts of nitrogenous fertilizer, phosphate fertilizer
6-10 parts, 10-15 parts of potash fertilizer, micro- 3-7 parts of fertilizer, 1-3 parts of plant growth-promoting substance, 4-8 parts of humic acid, 3-7 parts of bentonite.
2. tea tree special bio bacterial manure according to claim 1, it is characterised in that：It by following parts by weight raw material system
At：10 parts of Bacillus cercus microbial inoculum, 5 parts of bacillus subtilis microbial agent, 5 parts of trichoderma bacteria agent, 30 parts of organic fertilizer, nitrogenous fertilizer 12
Part, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 2 parts of plant growth-promoting substance, 6 parts of humic acid, 5 parts of bentonite.
3. tea tree special bio bacterial manure according to claim 1 or 2, it is characterised in that：The organic fertilizer is to arrange livestock and poultry
It is 5 to let out object, stalk, rubsen cake, peat soil, vinasse, Chinese medicine slag, edible fungi residue in mass ratio:2:1:1:0.5:0.5:0.5
Even mixing, and by water tariff collection in 30-35%, pH 6.0-6.8, the EM fermenting agents that mass percent is 2-3% are added, it is rotten
Ripe post-processing in 15-20 days is made.
4. tea tree special bio bacterial manure according to claim 1 or 2, it is characterised in that：The nitrogenous fertilizer refer to ammonium fertilizer,
The combination of one or more of nitrate fertilizer or amide nitrogen fertilizer；The phosphate fertilizer refers to phosphorus pentoxide, natural phosphate fertilizer
Or the combination of one or more of chemical phosphatic ferfilizer；The potash fertilizer refers in potassium chloride, potassium sulfate, plant ash, kainite
A combination of one or more；Micro- fertilizer for mass ratio is:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:
The mixed fertilizer of calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1；The plant growth-promoting substance is quality
Than for:1-2:1-2:Chitosan+amino acid+seaweeds of 0.1-0.2；The humic acid water content is 3-5%, and organic matter contains
Amount >=75%.
5. a kind of preparation method of tea tree special bio bacterial manure as claimed in claim 1 or 2, it is characterised in that：Including as follows
Processing step：
Step 1. takes Bacillus cercus microbial inoculum, bacillus subtilis microbial agent, trichoderma bacteria agent, organic fertilizer, nitrogen by regulation proportioning
Fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, plant growth-promoting substance, humic acid, bentonite, it is spare；
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly；
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, tea tree special bio bacterium is made in screened, drying, packaging
Fertilizer.
6. a kind of application of tea tree special bio bacterial manure as claimed in claim 1 or 2, it is characterised in that including following side
The application in face：(1) yield for increasing tea tree, improves tea tree quality；(2) induction enhancing tea tree disease resistance, effective prevention and control include tea
Root disease including tree root maize ear rot, root knot, root-knot nematode；(3) growth for promoting low age and the tea tree plant that grows into forest, improves tea
Tree root system micro organism quantity and soil enzyme activities.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108863618A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of tea tree selenium-rich liquid bacterial manure and the preparation method and application thereof |
| CN109650972A (en) * | 2018-12-28 | 2019-04-19 | 孙奕明 | A kind of organic-inorganic compound fertilizer and preparation method thereof |
| CN109761715A (en) * | 2019-03-29 | 2019-05-17 | 江城中澳农业科技发展有限公司 | A kind of Queensland nut special bio bacterial manure and preparation method thereof |
| CN111635282A (en) * | 2020-06-15 | 2020-09-08 | 重庆市农业科学院 | Special bacterial fertilizer for tea trees and preparation method thereof |
| CN112314639A (en) * | 2020-11-03 | 2021-02-05 | 广东省农业科学院茶叶研究所 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
| CN112552101A (en) * | 2020-09-30 | 2021-03-26 | 黄山市新安源有机茶开发有限公司 | Organic fertilizer for tea planting |
| CN113831183A (en) * | 2021-10-11 | 2021-12-24 | 思南五盛肥业有限责任公司 | Special biochar-based fertilizer for improving soil for tea trees and preparation method thereof |
| CN113875472A (en) * | 2021-09-16 | 2022-01-04 | 中南林业科技大学 | Tea oil tree planting method through soil improvement |
| CN115851502A (en) * | 2022-09-28 | 2023-03-28 | 云南大学 | Bacillusmycoides, microbial inoculum, liquid-state biological organic fertilizer and application of bacillus mycoides, microbial inoculum and liquid-state biological organic fertilizer |
| CN116836023A (en) * | 2023-06-30 | 2023-10-03 | 泰安市农业科学院 | Microbial fertilizer special for tea trees and preparation method thereof |
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2018
- 2018-06-29 CN CN201810692789.XA patent/CN108503466A/en not_active Withdrawn
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108863618A (en) * | 2018-09-19 | 2018-11-23 | 贵州大学 | A kind of tea tree selenium-rich liquid bacterial manure and the preparation method and application thereof |
| CN109650972A (en) * | 2018-12-28 | 2019-04-19 | 孙奕明 | A kind of organic-inorganic compound fertilizer and preparation method thereof |
| CN109761715A (en) * | 2019-03-29 | 2019-05-17 | 江城中澳农业科技发展有限公司 | A kind of Queensland nut special bio bacterial manure and preparation method thereof |
| CN111635282B (en) * | 2020-06-15 | 2022-04-22 | 重庆市农业科学院 | Special bacterial fertilizer for tea trees and preparation method thereof |
| CN111635282A (en) * | 2020-06-15 | 2020-09-08 | 重庆市农业科学院 | Special bacterial fertilizer for tea trees and preparation method thereof |
| CN112552101A (en) * | 2020-09-30 | 2021-03-26 | 黄山市新安源有机茶开发有限公司 | Organic fertilizer for tea planting |
| CN112314639A (en) * | 2020-11-03 | 2021-02-05 | 广东省农业科学院茶叶研究所 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
| CN113875472B (en) * | 2021-09-16 | 2023-02-21 | 中南林业科技大学 | Tea oil tree planting method through soil improvement |
| CN113875472A (en) * | 2021-09-16 | 2022-01-04 | 中南林业科技大学 | Tea oil tree planting method through soil improvement |
| CN113831183A (en) * | 2021-10-11 | 2021-12-24 | 思南五盛肥业有限责任公司 | Special biochar-based fertilizer for improving soil for tea trees and preparation method thereof |
| CN115851502A (en) * | 2022-09-28 | 2023-03-28 | 云南大学 | Bacillusmycoides, microbial inoculum, liquid-state biological organic fertilizer and application of bacillus mycoides, microbial inoculum and liquid-state biological organic fertilizer |
| CN115851502B (en) * | 2022-09-28 | 2023-06-30 | 云南大学 | Bacillus pseudomycoides, microbial inoculum and liquid bio-organic fertilizer and application thereof |
| CN116836023A (en) * | 2023-06-30 | 2023-10-03 | 泰安市农业科学院 | Microbial fertilizer special for tea trees and preparation method thereof |
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Application publication date: 20180907 |