CN108707035A - A kind of tea tree high-performance bio bacterial manure and the preparation method and application thereof - Google Patents

A kind of tea tree high-performance bio bacterial manure and the preparation method and application thereof Download PDF

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Publication number
CN108707035A
CN108707035A CN201810866789.7A CN201810866789A CN108707035A CN 108707035 A CN108707035 A CN 108707035A CN 201810866789 A CN201810866789 A CN 201810866789A CN 108707035 A CN108707035 A CN 108707035A
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parts
fertilizer
tea tree
tea
bacterial manure
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CN201810866789.7A
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Inventor
吴小毛
张承
龙友华
李姣红
王秋萍
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Guizhou University
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Guizhou University
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Priority to CN201810866789.7A priority Critical patent/CN108707035A/en
Publication of CN108707035A publication Critical patent/CN108707035A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a kind of tea tree high-performance bio bacterial manure and the preparation method and application thereof,, with 15 parts of microbial bacterial agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 3 parts of chitosan, 2 parts of amino acid, 2 parts of seaweeds, 6 parts of humic acid, 5 parts of bentonite for raw material, tea tree high-performance bio bacterial manure is made in agitated mixing, screening, drying, packaging for it.Enhance tea tree disease resistance applied to the yield, improvement tea tree quality, induction for increasing tea tree, improve tea root micro organism quantity and soil enzyme activities etc..Have many advantages, such as high significant effect, green non-pollution, utilization rate of fertilizer, improvement soil, prevention and control disease, improve yield and quality, and is of low cost, easy to spread.

Description

A kind of tea tree high-performance bio bacterial manure and the preparation method and application thereof
Technical field
The present invention relates to tree plant cultivation technologies and fertilising field, and in particular to a kind of tea tree high-performance bio bacterial manure and its preparation Method and application.
Background technology
The poor efficiency of chemical fertilizer and its high environmental risk brought are always to perplex the significant problem in the whole world in agricultural production, Chemical fertilizer use has also resulted in tea place ecological environment severe exacerbation in Tea Production, and tea leaf quality is difficult to ensure.How to make The special fertilizer of standby tealeaves green ecological, optimizes growth of tea plant environment, improves the yield and quality of tea tree, to promoting entire tea place to give birth to The improvement of state system is that tea industry sustainable development is of great significance.And the tea leaf quality of simple application of organic fertilizers production is good, But yield is not high.Although being increased substantially using the tealeaves yield of inorganic fertilizer production merely, quality is declined.And due to tea Tree is more batches of harvestings in 1 year, relatively continuous and steady to the absorption of nutrient, without obviously peak period.Therefore tea tree The fertilizer of application should fertilizer efficiency it is long, in order to avoid frequently fertilising cause root system to injure, nutrient supplies stabilization again, to ensure that blade is equal It is even, improve tea quality.And bio-bacterial manure is the important side of a kind of environmental friendly fertilizer and the future of agriculture sustainable development One of to, have and maintain and increase soil fertility, be conducive to the breeding of microorganism sociales, keep ecological balance, reduces ring Many advantages, such as border is polluted.
Currently, tea tree application fertilizer be mainly Common compound fertilizer and common organic fertilizer, rarely have in the prior art exclusively for The ideal biological bacterial manure of tea tree design.
Invention content
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, utilization rate of fertilizer be high, improvement Soil, the tea tree high-performance bio bacterial manure for meeting growth of tea plant nutritional need.
Technical scheme is as follows:
A kind of tea tree high-performance bio bacterial manure, it is made from the following raw materials in parts by weight:1, a kind of tea tree high-performance bio bacterial manure, It is characterized in that:It is made from the following raw materials in parts by weight:10-20 parts of microbial bacterial agent, 25-35 parts of organic fertilizer, nitrogenous fertilizer 10-15 Part, 8-12 parts of phosphate fertilizer, 10-15 parts of potash fertilizer, micro- 3-7 parts of fertilizer, 2-5 parts of chitosan, -3 parts of amino acid 1,1-3 parts of seaweeds, rotten plant 4-8 parts sour, 3-7 parts of bentonite.
Preferably, it is made from the following raw materials in parts by weight:15 parts of microbial bacterial agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, phosphorus 8 parts of fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 3 parts of chitosan, 2 parts of amino acid, 2 parts of seaweeds, 6 parts of humic acid, 5 parts of bentonite.
Wherein, the strain contained in the microbial bacterial agent is germ oligotrophy unit cell XQ08 (CCTCC NO:M 2015726), providencia stuartii JD (CCTCC NO:M 2017738) and monospore category bacterium J3 (CCTCCNO:M2017739) Mix bacterium agent;Its production method is:3 kinds of bacterial strains expand respectively connect cultivation and fermentation after, bacterium solution is adsorbed with sterile solid absorbent, By weight XQ08:JD:J3=3-5:2-3:1-2 is that standing 2d at dark is placed on after mixing well, then at 25~30 DEG C Under the conditions of dry, water tariff collection within 30% to get solid fungicide, living bacteria count >=10,000,000,000/gram.
Further, the solid absorbent is 3 by weight by maize straw powder+peanut shell powder+sawdust:2:1 It is made.
Further, organic fertilizer is by farm animal excrement, stalk, rubsen cake, vinasse, Chinese medicine slag, edible mushroom in the present invention Bacteria residue is 5 in mass ratio:2:1:0.5:0.5:0.5 uniformly mixing, and water tariff collection is added in 30-35%, pH 6.0-6.8 Enter the EM fermenting agents that mass percent is 2-3%, decomposed post-processing in 15-20 days is made.The nitrogenous fertilizer that the present invention uses refers to ammonium state The combination of one or more of nitrogenous fertilizer, nitrate fertilizer or amide nitrogen fertilizer;The phosphate fertilizer refers to phosphorus pentoxide, natural The combination of one or more of phosphate fertilizer or chemical phosphatic ferfilizer;Potash fertilizer refers in potassium chloride, potassium sulfate, plant ash, kainite A combination of one or more;Micro- fertilizer for mass ratio is:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:0.5-1's The mixed fertilizer of calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer;Chitosan is water solubility, and deacetylation is more than 95%;Amino acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, egg ammonia Appointing in acid, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate, cysteine The combination of five kinds of meaning or more;Seaweeds are pure natural marine algae extract;Humic acid water content be 3-5%, the content of organic matter >= 75%.
The preparation method of the tea tree high-performance bio bacterial manure of the present invention, comprises the technical steps that:
Step 1. takes microbial bacterial agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, chitosan, amino by regulation proportioning Acid, seaweeds, humic acid, bentonite, it is spare;
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly;
Under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, screened, drying, packaging are made tea tree and efficiently give birth to step 3. Object bacterial manure.
The bio-bacterial manure of the present invention can also be applied at least one of following (1)-(3):
(1) yield for increasing tea tree, improves tea tree quality;
(2) induction enhancing tea tree disease resistance, the diseases such as effective prevention and control tea tree root rot, root knot, root-knot nematode;
(3) growth for promoting low age and the tea tree plant that grows into forest, improves tea root micro organism quantity and soil enzyme activities.
Compared with prior art, the present invention bio-bacterial manure of the present invention is using microbial bacterial agent, organic fertilizer, plant growth-promoting object Matter, a great number of elements and trace element etc. form bio-bacterial manure, can effectively improve tea tree economic benefit, improving quality, promote plant Good growth and improvement root soil environment, and can effectively induce tea tree plant, the enhancing of tea reality disease resistance and prevent tea tree root-rot The generation of the great root disease such as disease, root knot, root-knot nematode, green non-pollution, utilization rate of fertilizer height notable with effect tea, It improves soil, prevention and control disease pest, improve the advantages that yield and quality, and is of low cost, easy to spread.
Specific implementation mode
With reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1:
1. bio-bacterial manure is formed and is prepared:
Bio-bacterial manure A formula compositions and preparation, (1) composition:15 parts of microbial bacterial agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, phosphorus 8 parts of fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 3 parts of chitosan, 2 parts of amino acid, 2 parts of seaweeds, 6 parts of humic acid, 5 parts of bentonite.(2) It prepares:By regulation proportioning take microbial bacterial agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, chitosan, amino acid, seaweeds, Humic acid, bentonite, it is spare;The above raw material is placed in blender by step to be thoroughly mixed uniformly;25-30 DEG C of temperature, Under conditions of pH 6.0-6.8, tea tree high-performance bio bacterial manure A is made in screened, drying, packaging.
Composite fertilizer's B formula compositions and preparation, (1) composition:It is 45 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, micro- 5 parts of fertilizer, 3 parts of chitosan, 2 parts of amino acid, 2 parts of seaweeds, 6 parts of humic acid, 5 parts of bentonite.(2) it prepares:It is taken by regulation proportioning Organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, amino acid, seaweeds, humic acid, bentonite, it is spare;Step sets the above raw material It is thoroughly mixed in blender uniformly;Under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, screened, drying, packaging Tea tree compound fertilizer B is made.
2. field trial:
Experimental field overview:Experimental field it is located at Guizhou Zhengan County tea tree garden, kind is " Zhengan County's white tea ", and mean sea level is 1276m, subtropical zone monsoon moist climate, has a moderate climate, and 15.2 DEG C average, abundant rainfall, long annual rainfall is in 1100-1300 Between millimeter, the hydro-thermal same period.It is yellow earth for examination tea garden soil type, random in tea place before experiment, multiple spot mixing acquisition depth 0- The soil sample of 60cm is as soil background, pH value 5.86, total nitrogen content 1.72gkg-1, the content of organic matter is 36.58g·kg-1, alkaline hydrolysis nitrogen content is 106.76mgkg-1, available phosphorus content 4.67mgkg-1, effective potassium content is 3.66mg·kg-1, effective iron content 55.36mgkg-1
Field experimental design:By the application of tea tree of tea tree high-performance bio bacterial manure, tea garden soil type is yellow earth.Tea place makes Used time, base manure and top dressing are respectively applied once, and base manure is applied mid-November current year, account for total fertilizer 60%, top dressing is mid-March next year It applies, accounts for the 40% of fertilizer total amount.As a contrast with conventional organic and inorganic fertilizer, the same bio-bacterial manure of dose.Experiment is set 3 times It repeats.
3. analysis project and method:
Yield and quality:Tea polyphenols contains in investigation dry tea yield, the young sprout fresh leaf water extraction content of tealeaves, fresh tea leaves Free aminoacid content, fresh tea leaves Determination of Chlorophyll total amount in amount, fresh tea leaves.Investigate breeding time tea tree root rot, moire blight, False smaller green leaf hopper incidence counts cumulative life-incidence or insect population number;Incidence (%)=100 × (morbidity strain number/investigation strain Number).
Soil root micro organism quantity and enzyme assay:Urease activity indophenols colorimetric method for determining, active unit is with 1g Soil sample hydrolyzes the milligram number of the ammonia nitrogen of generation to indicate under the conditions of 37 DEG C after reaction in 24 hours;Sucrose activity phosphorus Molybdic acid colorimetric method for determining, unit of enzyme activity is with (37 DEG C, 24 hours) meters of reduced sugar mg/g dry ground;Catalase is with using permanganic acid Potassium titration, the ml that unit of enzyme activity reacts post consumption 0.1mol/LKMnO4 solution with 1g soil samples in 25 DEG C, 20min are come It indicates;Alkaline phosphatase disodium phenyl phosphate colorimetric method for determining, unit of enzyme activity is with the 24 hours phenol (PhOH) generated of every gram of soil Milligram number indicates.Edaphon clump count is measured using colony counting method:Bacterium beef-protein medium culture 24 It is counted after hour;Fungi is counted with PDA culture medium culture;Actinomyces are counted using Gause I synthetic media culture.
4. embodiment effect:
From table 1 to table 3 as can be seen that can effectively increase tea cured leaf yield using tea tree high-performance bio bacterial manure, extremely significantly Fresh leaf water extraction, tea polyphenols, free amino acid and chlorophyll content are improved, the formation of tea bush productivity and changing for quality are promoted It is kind;And it can effectively reduce the generation of the diseases such as tea tree root rot, root knot, root-knot nematode;And soil enzyme activities can be improved and carried High soil microbe quantity.
Influence of the application bio-bacterial manure of table 1 to tea bush productivity quality
Note:With marking different lowercase letters after column data in P<0.05 time difference reaches the level of signifiance, different capitalizations It indicates in P<0.01 time difference is same up to pole level of signifiance following tables
To disease for tea plant, a situation arises for the application of table 2 bio-bacterial manure
Influence of the application bio-bacterial manure of table 3 to tea soil enzymatic activity and micro organism quantity
The specific application example of the above only present invention, the present invention also has other embodiments, all to use equivalent replacement Or the technical solution that equivalent transformation is formed, it all falls within protection domain of the presently claimed invention.

Claims (6)

1. a kind of tea tree high-performance bio bacterial manure, it is characterised in that:It is made from the following raw materials in parts by weight:Microbial bacterial agent 10- 20 parts, 25-35 parts of organic fertilizer, 10-15 parts of nitrogenous fertilizer, 8-12 parts of phosphate fertilizer, 10-15 parts of potash fertilizer, micro- 3-7 parts of fertilizer, 2-5 parts of chitosan, - 3 parts of amino acid 1,1-3 parts of seaweeds, 4-8 parts of humic acid, 3-7 parts of bentonite.
2. tea tree high-performance bio bacterial manure according to claim 1, it is characterised in that:It by following parts by weight raw material system At:15 parts of microbial bacterial agent, 30 parts of organic fertilizer, 12 parts of nitrogenous fertilizer, 8 parts of phosphate fertilizer, 12 parts of potash fertilizer, fertile 5 parts micro-, 3 parts of chitosan, amino 2 parts of acid, 2 parts of seaweeds, 6 parts of humic acid, 5 parts of bentonite.
3. tea tree high-performance bio bacterial manure according to claim 1 and the preparation method and application thereof, it is characterised in that:It is described micro- The strain contained in bacteria agent is the mixed of germ oligotrophy unit cell XQ08, providencia stuartii JD and monospore category bacterium J3 Combined bacteria agent;Its production method is:3 kinds of bacterial strains expand respectively connect cultivation and fermentation after, bacterium solution is adsorbed with sterile solid absorbent, by weight Amount compares XQ08:JD:J3=3-5:2-3:1-2 is that standing 2d at dark is placed on after mixing well, then in 25~30 DEG C of conditions Lower drying, water tariff collection within 30% to get solid fungicide, living bacteria count >=10,000,000,000/gram.
4. tea tree high-performance bio bacterial manure according to claim 1 or 2, it is characterised in that:The organic fertilizer is to arrange livestock and poultry It is 5 to let out object, stalk, rubsen cake, vinasse, Chinese medicine slag, edible fungi residue in mass ratio:2:1:0.5:0.5:0.5 uniformly mixing, and By water tariff collection in 30-35%, pH 6.0-6.8, it is added the EM fermenting agents that mass percent is 2-3%, decomposed 15-20 days Post-processing is made.The nitrogenous fertilizer refers to the combination of one or more of ammonium fertilizer, nitrate fertilizer or amide nitrogen fertilizer; The phosphate fertilizer refers to the combination of one or more of phosphorus pentoxide, natural phosphate fertilizer or chemical phosphatic ferfilizer;The potash fertilizer is Refer to the combination of one or more of potassium chloride, potassium sulfate, plant ash, kainite;Micro- fertilizer for mass ratio is:2-5: 1-2:1-2:1-2:1-2:0.5-1:0.5-1:Calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer of 0.5-1 Mixed fertilizer;The chitosan is water solubility, and deacetylation is more than 95%;The amino acid is proline, glycine, paddy ammonia Acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, Arbitrary five kinds or more of combination in alanine, serine, arginine, asparatate, cysteine;The seaweeds are Pure natural marine algae extract;The humic acid water content is 3-5%, the content of organic matter >=75%.
5. a kind of preparation method of tea tree high-performance bio bacterial manure as claimed in claim 1 or 2, it is characterised in that:Including as follows Processing step:
Step 1. takes microbial bacterial agent, organic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, micro- fertilizer, chitosan, amino acid, sea by regulation proportioning Algae element, humic acid, bentonite, it is spare;
The above raw material is placed in blender by step 2. to be thoroughly mixed uniformly;
For step 3. under conditions of 25-30 DEG C of temperature, pH 6.0-6.8, tea tree high-performance bio bacterium is made in screened, drying, packaging Fertilizer.
6. a kind of application of tea tree high-performance bio bacterial manure as claimed in claim 1 or 2, it is characterised in that including following side The application in face:(1) yield for increasing tea tree, improves tea tree quality;(2) induction enhancing tea tree disease resistance, effective prevention and control include tea Root disease including tree root maize ear rot, root knot, root-knot nematode;(3) growth for promoting low age and the tea tree plant that grows into forest, improves tea Tree root system micro organism quantity and soil enzyme activities.
CN201810866789.7A 2018-08-01 2018-08-01 A kind of tea tree high-performance bio bacterial manure and the preparation method and application thereof Withdrawn CN108707035A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110959458A (en) * 2020-01-08 2020-04-07 刘景欣 Green planting method for organic tea
CN111955259A (en) * 2020-08-27 2020-11-20 广西南亚热带农业科学研究所 Long-spike cutting breeding method for tea trees
CN111955258A (en) * 2020-08-26 2020-11-20 广西南亚热带农业科学研究所 Full-illumination spraying cutting seedling method for tea trees

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110959458A (en) * 2020-01-08 2020-04-07 刘景欣 Green planting method for organic tea
CN111955258A (en) * 2020-08-26 2020-11-20 广西南亚热带农业科学研究所 Full-illumination spraying cutting seedling method for tea trees
CN111955259A (en) * 2020-08-27 2020-11-20 广西南亚热带农业科学研究所 Long-spike cutting breeding method for tea trees

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Application publication date: 20181026