CN112314639A - Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof - Google Patents
Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof Download PDFInfo
- Publication number
- CN112314639A CN112314639A CN202011211442.2A CN202011211442A CN112314639A CN 112314639 A CN112314639 A CN 112314639A CN 202011211442 A CN202011211442 A CN 202011211442A CN 112314639 A CN112314639 A CN 112314639A
- Authority
- CN
- China
- Prior art keywords
- residue
- fertilizer
- biological
- knot nematode
- chinese medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/34—Aspergillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a biological pesticide fertilizer for preventing and treating root knot nematode disease and a preparation method thereof. The biological medicine fertilizer is prepared from the following components in percentage by weight: 10-30% of glutamic acid residue, 0.1-0.3% of microbial agent, 0.5-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue. The compound preparation is scientifically compounded, raw materials are cooperated, the application is simple, the compound preparation has good immune prevention and control effects on the tea seedling root knot nematode disease, meanwhile, pesticides, chemical fertilizers and labor cost are saved, the pollution to the environment is reduced, and the compound preparation has good application prospects.
Description
Technical Field
The invention belongs to the technical field of modern agriculture. More particularly, relates to a biological pesticide fertilizer for preventing and treating root knot nematode diseases and a preparation method thereof.
Background
Root-knot nematodes (Meloidogyne) are a highly specialized species of the xenotrophic phytopathogenic nematodes. The host range of the root knot nematode is wide, and currently, more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, bottle gourds, lettuce, Chinese cabbages and the like can be known to be damaged by the root knot nematode, wherein the number of the crops is nearly 40. Root-knot nematodes are mainly harmful to plant roots and are characterized by a more normal increase in lateral and fibrous roots and the formation of spherical or conical white nodules of varying sizes, sometimes in the form of marbles, on the fibrous roots of young roots. The overground part of the damaged plant grows short and slow, the color of the leaves is abnormal, the fruiting is less, the yield is low, and even the plant dies in advance. At the same time, it can also spread some fungal and bacterial diseases.
The meloidogyne is small in size and difficult to see by naked eyes, and is mostly distributed in 0-20 cm of soil, particularly the number of nematodes in 3-9 cm of soil is the largest. The propagation pathway and pathogenic conditions of root-knot nematodes are the overwintering of the larvae in the soil or of the eggs and female adults in the nodules. In the next spring the air temperature is higher than 10 ℃. The method comprises the steps of hatching 1-year larvae in egg shells after eggs live through winter, allowing molts to enter 2-year larvae and then climb out of the egg shells, spreading the larvae to young and tender root tips by water flow or agricultural tools, puncturing root surfaces and cells by using an anastomosis needle, penetrating the root surfaces and the cells into roots from the root epidermis, and secreting irritants to expand the root cells to form root knots. At this time, the 2 nd larva molts to become a 3 rd larva, and then molts for 1 time to become an adult. The female adults are just inside the galls and the male adults enter the soil. The larvae are often transported with the nursery stock for long-distance propagation. The soil temperature is 25-30 ℃, the relative humidity of the soil is 40-70%, the growth and development of the soil are suitable, and about 25-30 days are needed for completing the first generation. Therefore, the root knot nematode disease has the characteristics of difficult early discovery, difficult control, difficult eradication and the like.
For example, tea seedling root knot nematode disease, the pathogeny mainly comprises southern root knot nematode (Meloidogyne incognita) and peanut root knot nematode (Meloidogyne arenaria), which are distributed in each tea area of the country and mainly damage tea seedlings. Most of the seedlings occur at the roots of 1-2 year old seedlings and cutting seedlings, and the main roots and the lateral roots grow thin nodules, which are also called worm-thin, large soybean-like, small rapeseed-like, yellow brown, rough surface, and a plurality of thin nodules gather together, the fibrous roots are few or no, and the diseased roots are deformed. The cuttage seedlings are infected with diseases, the diseased roots are more and more densely gathered into a whole, the tissues are loose and easy to break, the overground part is thin and small, the leaves gradually turn yellow, and the leaves fall seriously, so that the whole plant is withered.
The Guangdong province is a big province for tea planting, and according to 2019 statistical data, the tea planting area reaches 6 million hectares, and the large-area planting of tea trees also brings rapid development of tea seedling industry. However, the abuse of chemical fertilizers and pesticides for decades pollutes soil, air and water, destroys the soil granular structure and the balance of soil nutrient elements, influences the growth and stress resistance of tea trees, causes the breeding of pathogenic bacteria, particularly has high incidence of root-knot nematodes of tea seedlings, and causes great economic loss to tea nursery.
At present, most of pesticides for preventing and treating tea seedling root knot nematode diseases have high toxicity, single function and great difficulty in prevention and treatment operation. For example, the mixture of the thioisothiocyanatomethane-type dazomethane, the carbofuran and the dichloline ester and the novel medicament products such as the fumigating alkene for replacing the forbidden pesticide methyl bromide can release the thioisothiocyanatomethane, namely cyanide ions to poison and die the nematodes, but the ideal effect can be achieved only by special application instruments and even distribution, and meanwhile, the dosage is large, the application method is complex, the time is long, the cost is high, and the application is generally not easy to accept by growers.
Therefore, the prevention and control of the root knot nematode disease become a difficult problem to be solved urgently in the current tea seedling breeding.
Disclosure of Invention
The invention aims to solve the technical problems of overcoming the defects and shortcomings of the prior art, and the biological pesticide fertilizer which has good control effect on root-knot nematodes, especially on meloidogyne incognita and peanut root-knot nematodes, is prepared by combining Chinese medicine residues, glutamic acid residues and paecilomyces lilacinus and performing processes such as fermentation.
The invention aims to provide a biological pesticide fertilizer for preventing and treating root knot nematode diseases and a preparation method thereof.
The above purpose of the invention is realized by the following technical scheme:
a biological pesticide fertilizer for preventing and treating root knot nematode disease is prepared from the following components in percentage by weight: 10-30% of glutamic acid residue, 0.1-0.3% of microbial agent, 0.5-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
Preferably, the biological medicine fertilizer is prepared from the following components in percentage by weight: 15-25% of glutamic acid residue, 0.15-0.25% of microbial agent, 1-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
Preferably, the biological medicine fertilizer is prepared from the following components in percentage by weight: 20% of glutamic acid residue, 0.15-0.25% of microbial agent, 1-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
Most preferably, the biological medicine fertilizer is prepared from the following components in percentage by weight: 20% of glutamic acid residue, 0.2% of microbial agent, 1.4% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
Preferably, the Chinese medicine residue is one or more of rhizoma Cibotii residue, herba Dianthi residue, and fructus evodiae residue.
More preferably, the rhizoma cibotii decoction dregs: and (3) fringed pink herb residues: the mass ratio of the fructus evodiae dregs is 3-6: 1-2: 1 to 2.
Or rhizoma cibotii dregs: the weight ratio of the fringed pink herb residues is 1-2: 1 to 2.
Or the fringed pink herb residues: the mass ratio of the fructus evodiae dregs is 3-5: 1 to 3.
Most preferably, rhizoma cibotii dregs: and (3) fringed pink herb residues: the mass ratio of the evodia rutaecarpa dregs is 2: 1: 1.
or rhizoma cibotii dregs: the weight ratio of the fringed pink herb residues is 1: 1.
or the fringed pink herb residues: the mass ratio of the evodia rutaecarpa dregs is 5: 3.
more preferably, the rhizoma Cibotii residue is residue obtained by extracting rhizoma Cibotii with water
The herba Dianthi residue is residue obtained by extracting herba Dianthi seed with ethyl acetate.
The evodia rutaecarpa residue is the residue obtained by extracting medicinal components from tender evodia rutaecarpa fruit by an ethyl acetate extraction method.
Preferably, the glutamic acid residue is a mixture of waste thalli and fermentation bottom byproducts after sodium glutamate is extracted from grain fermentation mother liquor in the monosodium glutamate production process.
Preferably, the microbial agent is an organic fermentation agent.
More preferably, the microbial agent comprises bacillus subtilis, aspergillus niger and yeast.
More preferably, the microbial agent comprises 1-5: 1-5: 2-6 of bacillus subtilis, aspergillus niger and yeast.
Preferably, the paecilomyces lilacinus refers to paecilomyces lilacinus granules.
Preferably, the disease applicable to the biological medicine fertilizer is a disease caused by meloidogyne incognita and/or meloidogyne arachidis.
As an alternative embodiment, the preparation method of the biological pesticide fertilizer comprises the following steps: crushing the traditional Chinese medicine residues, mixing and stirring the crushed traditional Chinese medicine residues, glutamic acid residues and a microbial agent, transferring the mixture into a fermentation tank after stirring uniformly, performing compost fermentation for 12-15 days, transferring the mixture into an aging and decomposing tank for 15-20 days to obtain a fermented material, crushing and screening the fermented material, adding paecilomyces lilacinus granules, performing granulation, drying and cooling after stirring uniformly to obtain the biological medicine fertilizer for preventing and treating the root knot nematode disease of the tea seedlings, detecting and packaging to obtain a finished product, and storing the finished product in a warehouse.
In the scheme of the biological pesticide fertilizer, the main components are as follows:
1. rhizoma Cibotii, named rhizoma Cibotii and monkey hair, is rhizoma Cibotii of family Hyriopsis cumingii, and contains starch and aspidinol; the aspidinol B is also called tridentate phenol, contains tannin, and can be used as pesticide for preventing and treating aphid and red spider. The experimental research of the invention finds that the rhizome of the cibotium barometz is extracted by water boiling, and the medicinal component obtained by extraction shows 100 percent of nematicidal rate within 72 hours.
2. Dianthus superbus, also known as Dianthus superbus, is a perennial herb of the genus Dianthus in the family Dianthus. The chemical components comprise 2.62 percent of crude protein, 13.18 percent of nitrogen-free extract, vitamin A substances and alkaloid, and the experimental study of the invention finds that the extract obtained by extracting the dianthus superbus with ethyl acetate has strong activity of killing the root-knot nematodes and shows 100 percent of nematode killing rate within 72 hours.
3. Evodia rutaecarpa, named as Evodia rutaecarpa, such as Evodia rutaecarpa, tea pepper, and Zanthoxylum piperitum. The fructus evodiae tender fruit contains 12 alkaloids, wherein furoquinolones, quinolones, acridones and indotropisoquinolones 4 are main traditional Chinese medicine plants which are ancient. The experimental research of the invention finds that the extract obtained by separating the tender fruit of evodia rutaecarpa through an ethyl acetate extraction method has very strong activity of killing the root-knot nematodes, and shows 100% of nematode killing rate within 72 hours.
4. Glutamic acid residue is produced by using starch as a raw material, urea, salt and the like as nitrogen sources, adding auxiliary materials and culturing glutamic acid producing bacteria. The mixture of the waste thalli and the fermentation bottom by-product after the sodium glutamate is extracted from the grain fermentation mother liquor in the production process of the monosodium glutamate. After the monosodium glutamate wastewater concentrated solution is cooled to room temperature, a large amount of ammonium sulfate crystals are separated out, the ammonium sulfate can be used as an inorganic fertilizer, and the residual concentrated solution can be further prepared into an organic fertilizer after glutamic acid is extracted. The high concentration waste water of monosodium glutamate contains rich nitrogen source and bioactive substances, and the technology for producing organic compound fertilizer by using waste water of monosodium glutamate belongs to the scientific and technological project of 'eight five' and 'nine five'. In addition, the pH value of the monosodium glutamate slag is 3.8-4.5, and the monosodium glutamate slag can play a role in acid-base balance and control of nitrogen volatilization in organic fertilizer fermentation.
5. The paecilomyces lilacinus is an effective parasitic bacterium of meloidogyne incognita and white cyst nematode eggs, and the inhibition mechanism of the paecilomyces lilacinus on the meloidogyne is that after the paecilomyces lilacinus is contacted with the nematode cyst, in a viscous matrix, biocontrol strain hyphae surround the whole egg, the tail end of the hyphae becomes thick, the surface layer of the egg shell is cracked due to the activity of exogenous metabolites and fungal chitinase, and then the fungus invades and replaces the egg shell. Can also secrete toxin to kill nematode. The egg parasitism rate of the paecilomyces lilacinus to the meloidogyne incognita is as high as 60-70%. The compound nematicide has control efficiency on various nematodes, and the parasites mainly comprise root-knot nematodes, cyst nematodes, golden nematodes, heterodera, and even intestinal ascarids of human and livestock, and is the most promising biocontrol preparation for controlling the root-knot nematodes.
The biological fertilizer is prepared by scientifically compounding and cooperating the raw materials and fermenting the raw materials, has good immune prevention and control effects on root knot nematode diseases (particularly diseases caused by meloidogyne incognita and peanut root knot nematodes, such as tea seedling root knot nematode diseases), saves pesticide, chemical fertilizer and labor cost and reduces pollution to the environment.
The application method of the biological pesticide fertilizer is simple: broadcasting 80-120 kg/667m of biological pesticide fertilizer when cutting tea seedlings2And uniformly cutting tea seedlings after rotary tillage and soil preparation are carried out for 15-20 cm, and watering after cutting.
The invention has the following beneficial effects:
the biological pesticide fertilizer has good immune prevention and control effects on root knot nematode diseases (particularly diseases caused by southern root knot nematode and peanut root knot nematode, such as tea seedling root knot nematode diseases).
The biological pesticide fertilizer combines the traditional Chinese medicine residues, the glutamic acid residues and the paecilomyces lilacinus, is scientific and reasonable, has wide main material sources and low production cost, uniformly operates pesticide application and fertilizer application, has a simple application method, is applied once in the whole growth period of tea seedlings, and has a very strong inhibition effect on the root-knot nematodes of the tea seedlings.
The method has the advantages of enhancing the root-knot nematode resistance and stress resistance of the tea seedlings, improving the soil fertility, improving the soil granular structure, reducing the cost of pesticide and fertilizer, saving labor, improving the control efficiency and the pesticide utilization rate and increasing the income of tea farmers.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 biological insecticide fertilizer
1. Preparation of biological pesticide fertilizer
1, biological pesticide fertilizer:
(1) 200kg of rhizoma cibotii dregs and 200kg of fringed pink dregs are crushed into powder, and then the powder, 100kg of glutamic acid dregs and 1.25kg of zymogen agent are put into a stirrer to be mixed and stirred for 3-5 minutes to obtain a fermentation raw material.
(2) And transferring the fermentation raw materials into a fermentation tank for composting fermentation for 12-15 days, then transferring into an aging and decomposing tank for 15-20 days to obtain a fermentation material, crushing, screening, adding 5kg of paecilomyces lilacinus granules, uniformly stirring, granulating and drying to obtain the biological medicine fertilizer for preventing and treating the root knot nematode disease of the tea seedlings.
And 2, biological pesticide fertilizer:
(1) 250kg of fringed pink herb residues and 150kg of fructus evodiae residues are selected and crushed into powder, then the powder, 100kg of glutamic acid residues and 0.75kg of zymogen agent are put into a stirrer to be mixed and stirred, and the fermentation raw material is obtained after stirring for 3-5 minutes.
(2) And transferring the fermentation raw materials into a fermentation tank for composting fermentation for 12-15 days, then transferring into an aging and decomposing tank for 15-20 days to obtain a fermentation material, crushing, screening, adding 6kg of paecilomyces lilacinus granules, uniformly stirring, granulating and drying to obtain the biological medicine fertilizer for preventing and treating the root knot nematode disease of the tea seedlings.
And (3) biological pesticide fertilizer:
(1) 200kg of rhizoma cibotii dregs, 100kg of fringed pink herb dregs and 100kg of fructus evodiae dregs are crushed into powder, and then the powder, 100kg of glutamic acid dregs and 1kg of zymogen are put into a stirrer to be mixed and stirred for 3-5 minutes to obtain a fermentation raw material.
(2) And transferring the fermentation raw materials into a fermentation tank for composting fermentation for 12-15 days, then transferring into an aging and decomposing tank for 15-20 days to obtain a fermentation material, crushing, screening, adding 7kg of paecilomyces lilacinus granules, uniformly stirring, granulating and drying to obtain the biological medicine fertilizer for preventing and treating the root knot nematode disease of the tea seedlings.
2. The execution standard of the biological pesticide fertilizer is as follows:
through quality detection, the product of the invention meets the technical specifications of national agricultural rural department industry standard organic fertilizers (NY525-2012), bio-organic fertilizers (NY884-2012) and granular drug fertilizers (NY/T3589-2020).
3. The application method of the biological pesticide fertilizer comprises the following steps:
broadcasting 80-120 kg/667m of biological pesticide fertilizer when cutting tea seedlings2And uniformly cutting tea seedlings after rotary tillage and soil preparation are carried out for 15-20 cm, and watering after cutting.
Example 2 field control Effect of biological insecticide-fertilizer on root-knot nematodes of tea seedlings
1 materials and methods
1.1 test agent
In order to verify the effect of the biological pesticide fertilizer (taking the biological pesticide fertilizer 3 in the embodiment as an example) and the dazomet, the abamectin and the smoked streakline missible oil on killing the root-knot nematodes, 3 seedling raising fields are selected in Guangdong province, Yuandong province and North district of Guangdong province for testing, the fertility of the test fields is medium, and the root-knot nematodes are serious emergence areas throughout the year. Wherein, Mezhou is the No. 1 test place, Huizhou is the No. 2 test place, and Ender is the No. 3 test place.
1.2 test methods
Each area selects a test field area 667m2Wherein the CK region is 67m2Area of each cell 150m2About 16000 seedlings of tea seedlings are planted in each cell.
(1) Administering 98% dazomet microgranule 30kg/667m in the cell 40 days ahead (2 days and 25 days in 2020)2Uniformly watering after rotary tillage and soil preparation are carried out for 15-20 cm in depth, covering a film for sealing and fumigating for 28 days after the soil humidity is kept to be 60-70%, uncovering the film, carrying out tillage again and aerating for 12 days, and then carrying out tea seedling cuttage.
(2) The dosage of 1.8 percent avermectin missible oil in a cell is 0.5L/667m2The application method comprises diluting the tea seedlings with water and spraying the diluted solution on the soil surface. 1.8% of abamectinThe emulsifiable solution is applied for 1 time after 2 months, the dosage is the same as that of the 1 st time, and the application mode is root irrigation.
(3) The fumigating linear alkene district is applied with 92% fumigating linear alkene missible oil 9kg/667m 14 days ahead (3 days and 21 days in 2020)2When the tea tree seedling cuttage seedling cultivation device is used, after soil is leveled, a soil injector is used for injecting pesticides, then soil covering, film covering, sealed fumigation and uncovering are carried out for 7 days, soil loosening and air dissipation are carried out for 7 days, and then tea seedlings are cut.
(4) In the biological pesticide fertilizer plot, 80kg/667m of biological pesticide fertilizer is scattered when the tea seedlings are cut2And cutting tea seedlings and watering after rotary tillage and soil preparation are uniform to 15-20 cm in depth. The biological pesticide fertilizer is applied for 1 time only when the tea seedlings are inserted.
The experiment is carried out by applying the medicine and cutting the tea seedlings at 4-5 months in 2020, investigating the root disease degree of all plants in the cell at 8-5 months in 2020, and calculating the disease index and the prevention effect. The data analysis adopts Duncan new complex pole difference method.
2 results and analysis
The results are given in Table 1 below
TABLE 1 field control effect of the pesticide fertilizer on tea seedling root-knot nematode
The results show that the control effects of the treatments on the tea seedling root-knot nematode are as follows from high to low in sequence: the biological pesticide fertilizer is larger than dazomet, larger than smoked alkene missible oil and larger than avermectin, wherein the average prevention and treatment effect of the biological pesticide fertilizer on the root-knot nematode is 84.13 percent, which is obviously higher than that of other treatments (P is smaller than 0.05). And the pesticide is only applied once during the cuttage of the tea seedlings, so that the pesticide has a very good effect of preventing and treating the root-knot nematodes of the tea seedlings.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The biological pesticide fertilizer for preventing and treating root knot nematode disease is characterized by being prepared from the following components in percentage by weight: 10-30% of glutamic acid residue, 0.1-0.3% of microbial agent, 0.5-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
2. The adhesive is characterized by being prepared from the following components in percentage by weight: 15-25% of glutamic acid residue, 0.15-0.25% of microbial agent, 1-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
3. The adhesive is characterized by being prepared from the following components in percentage by weight: 20% of glutamic acid residue, 0.15-0.25% of microbial agent, 1-1.5% of paecilomyces lilacinus and the balance of traditional Chinese medicine residue.
4. The biological medicine fertilizer as claimed in any one of claims 1 to 3, wherein the Chinese medicine residue is one or a mixture of more than two of rhizoma cibotii residue, herba dianthi residue and fructus evodiae residue; the rhizoma Cibotii residue is residue obtained by extracting rhizoma Cibotii with water to obtain medicinal components; the fringed pink herb residue is the residue obtained by extracting medicinal components from fringed pink seeds by an ethyl acetate extraction method; the evodia rutaecarpa residue is the residue obtained by extracting medicinal components from tender evodia rutaecarpa fruit by an ethyl acetate extraction method.
5. The biological insecticide-fertilizer according to any one of claims 1 to 3, wherein said glutamic acid residue is a mixture of waste bacteria and fermentation bottom by-products obtained after extracting sodium glutamate from a grain fermentation mother liquor in a monosodium glutamate production process.
6. The biological medicine fertilizer as claimed in any one of claims 1 to 3, wherein the microbial agent is an organic matter fermenting agent.
7. The biological insecticide fertilizer according to claim 6, wherein the main components are Bacillus subtilis, Aspergillus niger and yeast.
8. The biological medical fertilizer as claimed in any one of claims 1 to 3, wherein the paecilomyces lilacinus is paecilomyces lilacinus granule.
9. The biological pesticide fertilizer as claimed in any one of claims 1 to 3, wherein the root knot nematode disease is a disease caused by Meloidogyne incognita and/or Meloidogyne arachidis.
10. The biological pesticide fertilizer according to any one of claims 1 to 3, wherein the biological pesticide fertilizer for preventing and treating the root knot nematode disease of the tea seedlings is prepared by crushing the traditional Chinese medicine residues, mixing and stirring the crushed traditional Chinese medicine residues, the glutamic acid residues and the microbial agent, uniformly stirring the mixture, transferring the mixture into a fermentation tank for composting and fermenting for 12 to 15 days, transferring the mixture into an aging and decomposing tank for 15 to 20 days to obtain a fermented material, crushing and screening the fermented material, adding the paecilomyces lilacinus granules, uniformly stirring the mixture, granulating, drying and cooling the mixture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011211442.2A CN112314639A (en) | 2020-11-03 | 2020-11-03 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011211442.2A CN112314639A (en) | 2020-11-03 | 2020-11-03 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112314639A true CN112314639A (en) | 2021-02-05 |
Family
ID=74324604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011211442.2A Pending CN112314639A (en) | 2020-11-03 | 2020-11-03 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112314639A (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165016A (en) * | 2007-10-12 | 2008-04-23 | 马玉新 | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof |
| CN101803546A (en) * | 2010-03-25 | 2010-08-18 | 陕西农产品加工技术研究院 | Biodegradable agricultural mulching film capable of preventing and controlling root-knot nematodes and preparation method thereof |
| CN104987223A (en) * | 2015-07-21 | 2015-10-21 | 刘奋安 | Organic fertilizer using Chinese medicine residues as raw materials for insect prevention and disease resistance, and preparation method thereof |
| US20160174554A1 (en) * | 2014-12-18 | 2016-06-23 | Derivados Biotecnologicos Sac Derbiotec | Compostion and method for the control of phyto-parasitic nematodes |
| CN106083428A (en) * | 2016-06-15 | 2016-11-09 | 江西天人生态股份有限公司 | A kind of microbial organic fertilizer and preparation method thereof |
| CN106810362A (en) * | 2017-01-17 | 2017-06-09 | 河南漯效王生物科技股份有限公司 | A kind of composite microbiological fertilizer of reduction underground root-knot nematode and preparation method thereof |
| CN106866292A (en) * | 2017-02-27 | 2017-06-20 | 青岛海之星生物科技有限公司 | A kind of organic fertilizer for preventing and treating peanut root-knot nematode and preparation method thereof |
| CN107619308A (en) * | 2016-07-13 | 2018-01-23 | 湖南泰谷生物科技股份有限公司 | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application |
| CN108503466A (en) * | 2018-06-29 | 2018-09-07 | 贵州大学 | A kind of tea tree special bio bacterial manure and the preparation method and application thereof |
| CN108658702A (en) * | 2018-06-14 | 2018-10-16 | 安徽懒农科技有限责任公司 | A kind of bio-organic fertilizer special material for root knot nematode control |
| CN108727124A (en) * | 2018-08-07 | 2018-11-02 | 重庆富农现代生物科技有限公司 | A kind of multifunctional biological fertilizer and preparation method thereof of prevention root-knot nematode |
| CN108976065A (en) * | 2018-07-26 | 2018-12-11 | 山东土秀才生物科技有限公司 | A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode |
| CN109096004A (en) * | 2018-09-05 | 2018-12-28 | 福建省致青生态环保有限公司 | A kind of preparation method and application of bio-feritlizer that preventing and treating tea tree nematode |
| CN110463551A (en) * | 2019-07-25 | 2019-11-19 | 山东省农业科学院植物保护研究所 | A kind of biological control method of groundnut root knot nematode disease |
-
2020
- 2020-11-03 CN CN202011211442.2A patent/CN112314639A/en active Pending
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165016A (en) * | 2007-10-12 | 2008-04-23 | 马玉新 | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof |
| CN101803546A (en) * | 2010-03-25 | 2010-08-18 | 陕西农产品加工技术研究院 | Biodegradable agricultural mulching film capable of preventing and controlling root-knot nematodes and preparation method thereof |
| US20160174554A1 (en) * | 2014-12-18 | 2016-06-23 | Derivados Biotecnologicos Sac Derbiotec | Compostion and method for the control of phyto-parasitic nematodes |
| CN104987223A (en) * | 2015-07-21 | 2015-10-21 | 刘奋安 | Organic fertilizer using Chinese medicine residues as raw materials for insect prevention and disease resistance, and preparation method thereof |
| CN106083428A (en) * | 2016-06-15 | 2016-11-09 | 江西天人生态股份有限公司 | A kind of microbial organic fertilizer and preparation method thereof |
| CN107619308A (en) * | 2016-07-13 | 2018-01-23 | 湖南泰谷生物科技股份有限公司 | Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application |
| CN106810362A (en) * | 2017-01-17 | 2017-06-09 | 河南漯效王生物科技股份有限公司 | A kind of composite microbiological fertilizer of reduction underground root-knot nematode and preparation method thereof |
| CN106866292A (en) * | 2017-02-27 | 2017-06-20 | 青岛海之星生物科技有限公司 | A kind of organic fertilizer for preventing and treating peanut root-knot nematode and preparation method thereof |
| CN108658702A (en) * | 2018-06-14 | 2018-10-16 | 安徽懒农科技有限责任公司 | A kind of bio-organic fertilizer special material for root knot nematode control |
| CN108503466A (en) * | 2018-06-29 | 2018-09-07 | 贵州大学 | A kind of tea tree special bio bacterial manure and the preparation method and application thereof |
| CN108976065A (en) * | 2018-07-26 | 2018-12-11 | 山东土秀才生物科技有限公司 | A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode |
| CN108727124A (en) * | 2018-08-07 | 2018-11-02 | 重庆富农现代生物科技有限公司 | A kind of multifunctional biological fertilizer and preparation method thereof of prevention root-knot nematode |
| CN109096004A (en) * | 2018-09-05 | 2018-12-28 | 福建省致青生态环保有限公司 | A kind of preparation method and application of bio-feritlizer that preventing and treating tea tree nematode |
| CN110463551A (en) * | 2019-07-25 | 2019-11-19 | 山东省农业科学院植物保护研究所 | A kind of biological control method of groundnut root knot nematode disease |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104987223B (en) | It is a kind of using Chinese medicine slag as insect prevention disease-resistant organic fertilizer of raw material and preparation method thereof | |
| KR101431787B1 (en) | Organic Ginseng and the Method of Cultivation thereof | |
| CN103960031A (en) | Method for planting Maca applicable to high and cold high-altitude areas in Pamirs | |
| CN107723258B (en) | Composite microbial inoculum for preventing and treating tobacco bacterial wilt and preparation method thereof | |
| CN112753516B (en) | Ecological cultivation method for intercropping bighead atractylodes rhizome and soybeans | |
| CN108892562A (en) | A kind of composite biological medicinal fertilizer and the preparation method and application thereof improving quality of tobacco | |
| CN106977350A (en) | A kind of bletilla striata bio-feritlizer and preparation method thereof | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| Kumawat et al. | Growth and development of groundnut (Arachis hypogaea) under foliar application of panchgavya and leaf extracts of endemic plants | |
| CN106105632A (en) | A kind of planting technology of Rhizoma Dioscoreae | |
| CN108409479A (en) | Nematicidal microbial organic fertilizer and its preparation method and application | |
| CN110963849A (en) | Special fertilizer for paris polyphylla and preparation and application methods thereof | |
| CN104285772A (en) | Cultivating substrate and preparation method thereof and ginseng planting method | |
| CN105130654A (en) | Composite microbial fertilizer and production process of composite microbial fertilizer | |
| CN108203353A (en) | A kind of desinsection bio-organic fertilizer and preparation method thereof | |
| CN111517864A (en) | Special biological organic fertilizer for preventing and treating nematodes and preparation method thereof | |
| CN107602277A (en) | It is a kind of to prepare the method for biological organic fertilizer and the application on citrus bio-organic fertilizer special using tobacco stem discarded object | |
| CN106518433A (en) | Special citrus fertilizer for controlling underground pests and preparation method of special citrus fertilizer | |
| CN108191504B (en) | Preparation method and use method of special fertilizer for konjak | |
| CN111072420A (en) | Compound amino acid microbial agent and preparation method thereof | |
| CN112830852A (en) | Organic fertilizer with insect expelling effect and preparation method and application thereof | |
| CN105960975A (en) | Method for self-breeding and culturing potato virus-free ancestor by peasant household | |
| CN106064992A (en) | A kind of compound organic and inorganic fertilizer and preparation method thereof | |
| KR101639552B1 (en) | Forest Ginseng Cultivated From Wild Ginseng Berry And Cultivation Method Thereof | |
| CN108840752A (en) | A kind of biological organic fertilizer containing complex micro organism fungicide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |