CN108727124A - A kind of multifunctional biological fertilizer and preparation method thereof of prevention root-knot nematode - Google Patents

A kind of multifunctional biological fertilizer and preparation method thereof of prevention root-knot nematode Download PDF

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Publication number
CN108727124A
CN108727124A CN201810888495.4A CN201810888495A CN108727124A CN 108727124 A CN108727124 A CN 108727124A CN 201810888495 A CN201810888495 A CN 201810888495A CN 108727124 A CN108727124 A CN 108727124A
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fermentation
fertilizer
heap
microbial inoculum
organic fertilizer
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吕敦辉
姚渝
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Chongqing Funong Modern Biological Technology Co Ltd
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Chongqing Funong Modern Biological Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

Abstract

The invention discloses a kind of multifunctional biological fertilizers and preparation method thereof with biological control root-knot nematode.Mainly the material of following weight percent and microbial inoculum composition, the material include the organic fertilizer:Dregs of beans 5%-10%, rapeseed meal 2.5-5%, humic acid 25%-35%, vinasse 5-15%, chaff powder 15-30%, wheat bran 5-10%, sawdust powder 5-10% and moisture 10-25%, the microbial inoculum is by bacillus amyloliquefaciens (0.5-2.0%);Colloid bacillus cereus (0.1-0.5%);Paecilomyces lilacinus (0.1-0.5%) forms.The multifunctional biological fertilizer of the present invention can also be achieved nutrient laden, attachmentization other than it can kill root-knot nematode, small microenvironment remakes for soil natural environment, it is made to breed and colonize growth on a variety of nature cultures, it keeps efficacy lasting, improves soil soil property, promote organic matter.

Description

A kind of multifunctional biological fertilizer and preparation method thereof of prevention root-knot nematode
Technical field
The invention belongs to agricultural and biotechnology, a kind of specific multifunctional bio of biological control root-knot nematode is organic Fertilizer and preparation method thereof.
Background technology
In the late three decades, grain yield is increased production every year in China, and main contributions are largely to use chemical fertilizer and production hormone Equal products promote plant growth, while using pesticide disease and pest control.It data show, China's applying quantity of chemical fertilizer has accounted at present The 35% of global total amount is arrived, applying quantity of chemical fertilizer of ploughing per acre is up to 30~100 kilograms.Nearly 30 years from 1981 to 2008 year Between, China's grain yield increases 63%, and the usage amount of same period nitrogenous fertilizer then increases nearly 2 times, and the total dosage of chemical fertilizer ranks world head Position.However, the long-term excessive application of chemical fertilizer also brings a series of problem.It is exactly the decline of soil fertility, great Liang Geng first There is soil hardening in ground, the serious phenomenon of degenerating, and soil is also lower and lower to the utilization ratio of fertilizer, and soil-borne disease constantly goes out It is existing, also affect the quality of agricultural product.Followed by the pollution of environment, the application of nitrogenous fertilizer increases the discharge of greenhouse gases, together When, excess fertilizer outflows with water, and into underground water or rivers and lakes, causes eutrophication.Due to these chemical fertilizer, pesticide and production hormone It is that contemporaneity uses Deng nearly all, auxiliary additives such as dimethylbenzene therein etc. repeatedly remain, and make the soil knot in many places Structure, land fertility and plant ecological are unbalance, degeneration and mutation occurs, and cause crops especially vegetables, fruit tree etc. to occur obstinate Venereal disease is done harm to, and administers the pollution that the chemical pesticide used in these diseases increases soil and agricultural product.In this case, people Start consider find and study some while there is the fertilizer or agricultural additives of drug effect and fertilizer efficiency, to reduce chemical fertilizer and pesticide Amount of application, cost-effective, the purpose reducing work hours, reduce land pollution reached.But it is typically simply by pesticide and fertilizer The complexing agent of material formula is mixed, and produced by certain technique according to a certain percentage pesticide and fertilizer, the two is respectively Individually play its effectiveness, but between have no interaction.
Nemas is a kind of root disease caused by coelenterate nematode, seriously affects crop yield.Currently, plant Getting worse occurs for nematodiasis, and almost all of crop has eelworm harm.Parasitic nematode becomes the weight of plant infectious diseases One of pathogen is wanted, is had a significant impact to whole world agricultural production.It is estimated that the whole world is every year because eelworm harm is to grain and fibre Loss caused by tieing up crop is about 12.3%, causes about 100,000,000,000 dollars of loss.China's Agricultural planting is every year because of line Nearly 23,300,000,000 yuan of damage losses caused by worm.In the prevention of nematode it is most widely used be using nematicide carry out chemical prevention, but The usual toxicity of nematicide is higher, can increase the pollution of environment, and more and more scholars recognize that control parasitic nematode is necessary It takes aggregate measures or uses the Sustainable Treatment system using biological and ecological prevention as core.Fungi is ground in biocontrol of nematodes Study carefully more natural enemy biology and mostly important one of bio-control factors.
CN105859377 discloses a kind of Se-enriched bio-organic fertilizer, consists of the following compositions:40~80 parts of organic detritus, 0.01~0.1 part of selenium-containing compound, 10~15 parts of nitrogen, phosphorus and potassium fertilizer, 0.5~1 part of microbial bacterial agent, 5~10 parts of auxiliary agent, it is described micro- Bacteria agent by bacillus licheniformis, Paecilomyces lilacinus, bacillus amyloliquefaciens form but in terms of reducing root-knot nematode and It can't be satisfactory in terms of improving yield.
In today of resource constraint growing tension, the agricultural production in China's future is faced with dual-pressure, on the one hand to protect Barrier grain continually and steadily increases production, the sustainable development that on the other hand reduce pollution of area source, ensure agricultural, how to use fertilizer, becomes In order to which China's research work, enterprise and peasant must face and solve the problems, such as.The multi-functional characteristic that microbial manure has, Compared with conventional fertilizers, microbial manure in protecting ecology, the agricultural wastes utilization of resources, improve utilization rate of fertilizer and make produce Amount quality etc. has a clear superiority.
Invention content
The object of the present invention is to provide a kind of multifunctional biological fertilizer of biological control root-knot nematode and its preparations Method.The multifunctional biological fertilizer of the present invention, microbial inoculum are attached to nutrient matrix carrier, pass through biological organic fertilizer mu amount of application Greatly, microbial inoculum is easy to use, and rational matrix formulations collocation is conducive to the beneficial bacteriums such as Paecilomyces lilacinus and breeds and colonize in the soil life It is long.
A kind of multifunctional biological fertilizer of prevention root-knot nematode of the present invention, is mainly made of following material and microbial inoculum, Include the raw material of following proportioning:The material includes:Dregs of beans 5-10%, rapeseed meal 2.5-5%, humic acid 25%-35%, vinasse 5- 15%, chaff powder 15-30%, wheat bran 5-10%, sawdust powder 5-10% and moisture 10-25%, the microbial inoculum is by solution starch gemma bar Bacterium 0.5-2.0%, colloid bacillus cereus 0.1-0.5%, Paecilomyces lilacinus 0.1-0.5% compositions, accounting is material total weight pair Answer potency.
The biological organic fertilizer of aforementioned present invention, the preferred material includes dregs of beans 5%-10%, rapeseed meal 2.5-5%, corruption Sour 25%-30%, vinasse 10-15%, chaff powder 15-20%, wheat bran 5-10%, sawdust powder 5-10% and moisture 15-20% are grown, In the carbon content that can directly utilize be 8-20%, the nitrogen quantity that can directly utilize is 0.3-1.2%.
The biological organic fertilizer of aforementioned present invention, the microbial inoculum, precentagewise counts, by bacillus amyloliquefaciens 1.0- 1.5%, colloid bacillus cereus 0.2-0.3% and Paecilomyces lilacinus 0.25-0.4% compositions, content is to account for the weight of entire organic fertilizer Measure percentage.
A kind of method of multifunctional biological fertilizer preparing prevention root-knot nematode of the present invention, includes the following steps:
(1) activation of fermenting microbe
By No. 1 fermenting microbe of rich peasant and water according to 1:50 weight ratio mixing, activation are fermented for 12 hours or so to get activation Strain;Period is preferably sufficiently stirred 1-2 times, is mixed with fermentation materials after the dilution that can be added to the water later.
(2) preparation of fermentation materials
A) after mixing by raw soybean dregs, rapeseed meal, vinasse, chaff powder, wheat bran, sawdust powder, the work that step (1) obtains is added Change fermenting microbe, water is added after mixing, controls moisture;
B) windrow
Upper step is mixed with fermenting microbe material mix after pile fermentation heap, the height of the fermentation heap is 0.8-1.5 meters Left and right, width are 2 meters or so, and length is at 2~4 meters or more, 15 DEG C of environment temperature or less, with covering (preferred film or grass Curtain is as covering), when internal temperature is raised to 30 DEG C, covering is opened, heap temperature is begun to tip over when rising to 60 DEG C, overturns 2 ~3 times.
C) when second of overturning, heap temperature drop is low, again inoculation fermentation strain, second of fermentation, further comprises first No. 1 fermenting microbe of rich peasant is mixed and made into strain mixture with suitable chaff powder or wheat bran, then again by strain mixture and hair The mixed fermentation of ferment material keeps material fermentation abundant, wherein suitable chaff powder or wheat bran (can be reserved from dispensing) are Finger adds 1-2kg chaff powders per 10g fermenting microbes, and either (i.e. fermenting microbe and chaff powder or the weight ratio of wheat bran are 1 to wheat bran:100- 200)。
D) heap temperature is reduced after fermentation fully, material is loose, after fermentation maturity, controls moisture, screening is spare after crushing.
(3) microbial inoculum is added in decomposed good fermentation materials, is mixed 3-5 minutes.
(4) after mixing, humic acid is added, mixes 3-5 minutes again, is uniformly mixed and obtains organic fertilizer finished product.
The method of aforementioned present invention, second of access fermenting microbe of step c), can adequately ferment in this way, meanwhile, Under normal circumstances, heap temperature will appear 2~3 65 DEG C or more of high temperature during the fermentation, be needed when heap temperature is higher than 65 DEG C or more It repeatedly to overturn.
The method of aforementioned present invention, No. 1 fermenting microbe of the rich peasant includes careless bacillus and saccharomyces cerevisiae, dosage are Every 1 ton of material adds No. 1 fermenting microbe of 5-15g rich peasant, preferably 10g.
The method of aforementioned present invention controls moisture described in step a), and hand is made to grab the agglomerating no water droplet of material, webs water breakthrough print But it does not drip, landing can scatter.
The method of aforementioned present invention, the environment temperature of fermentation heap is preferably at 15 DEG C or more, and when heap temperature is more than 65 DEG C, need are multiple It overturns.
The principle of the invention is as follows:
Paecilomyces lilacinus condition of culture:The bacterium is of less demanding to nutritional condition, can be bred on a variety of nature cultures with Colonize growth, such as agricultural and sideline product waste material, waste residue and plant leaf blade etc..All kinds of oilseeds dregs of beans are all suitable culture materials.It should Bacterium is introduced into soil, often by many physics, the influence of chemistry and biological factor.The temperature of soil, moisture are organic Matter content, N-P-K content, acid-base value, salt content, trace element, other microorganisms of pesticide etc..It is not easy to breed in the soil.It causes Keep biological agent unstable to plant soil-borne diseases preventive effect.Biocontrol microorganisms are used in combination with organic substance, by rational natural Matrix material is arranged in pairs or groups, and biofermentation mode may be implemented nutrient laden, attachmentization, small microenvironment, acts on soil natural environment, Keep its efficacy lasting.The Paecilomyces lilacinus mechanism of action:Parasitic root-knot nematode worm's ovum, the time of infection rate and ovum and Fungal contact are long Short related, amount is also related.Spore is manured into soil, and mycelia is grown after sprouting, and mycelia encounters worm's ovum secretion enzyme and destroys periostracum, It penetrates chorion and enters mass propagation in ovum, break oophyte.And second year third year still has the function of partial control.Since organic fertilizer exists Soil improvement, soil organism promotion etc. left and right protrude, and part is applied throughout the year instead of chemical fertilizer at present, and enrichment beneficial bacterium exists Nematicidal, improves soil microbial activities, and effect is big.It is better after enrichment.
Compared with prior art, the present invention haing the following advantages:
1) microbial inoculum is attached in nutrient matrix carrier, and big by biological organic fertilizer mu amount of application, microbial inoculum is easy to use, rationally Matrix formulations collocation be conducive to the beneficial bacteriums such as Paecilomyces lilacinus and breed and colonize in the soil growth.
2) the high-efficiency broad spectrum biology nematocide function of Paecilomyces lilacinus is combined, and is not developed immunity to drugs, is matched and other phosphorus decomposings The colloid gemma and its bacillus amyloliquefaciens of potassium decomposing are made and have multi-functional complex biological organic fertilizer.To promoting crop growth Long, volume increase, construction green non-pollution agricultural, social benefit, ecological benefits are notable.
Specific implementation mode
Following embodiment is used to further understand and illustrate the essence of the present invention, but do not limit the invention in any way Range.
The multifunctional biological fertilizer of 1 biological control root-knot nematode of embodiment, is made of the material of following weight percents:
Material proportion:Dregs of beans:5%, rapeseed meal:5%, humic acid:25%, vinasse:10%, chaff powder:20%, wheat bran:10%, Sawdust powder:5%, appropriate amount of water.
Microbial inoculum matches:Bacillus amyloliquefaciens (1.5%);Colloid bacillus cereus (0.2%);Paecilomyces lilacinus (0.25%).
Preparation method is as follows:
(1) preparation of fermenting microbe
Use front activating:By rich peasant No. 1 fermenting microbe 1g, water according to 1:50 ratio, activation 12 hours or so, during which most It is sufficiently stirred 1-2 times well.It is mixed with fermentation materials after being diluted with water after activation.
(2) preparation of fermentation materials
A. by raw soybean dregs, rapeseed meal, vinasse, chaff powder, wheat bran, sawdust powder weighs by weight percentage, after rear addition activation Rich peasant's No. 1 fermenting microbe mixing, moisture control grabs the agglomerating no water droplet of material in 50%-60%, with hand, webs water breakthrough print but not It drips, landing, which can scatter, to be advisable, and during fermentation materials, whether moisture is suitable for very important.
b:Heap material heap is high
When doing pile fermentation heap, height is generally at 0.8-1.5 meters or so, and 2 meters or so of width, length is at 2~4 meters or more Heap ferment effect it is preferable.When 15 DEG C of environment temperature or less, covered with film or straw screen or mat, it, will when internal temperature is raised to 30 DEG C Covering is opened.Heap temperature is begun to tip over when rising to 60 DEG C, is turned over 2~3 times, and cooling if heap temperature is more than 65 DEG C, need to be overturned repeatedly, one As in the case of, will appear 2~3 65 DEG C or more of high temperature during the fermentation.
When second of turning, heap temperature, again inoculation fermentation strain are reduced, second is carried out and ferments, i.e., first by rich peasant 1 Number fermenting microbe 1g is uniformly mixed with the rice bran (fermentation materials 100KG of this example or so) of 100g, strain mixture is made, then Again by strain mixture and fermentation materials mixed fermentation, material is made adequately to ferment.
After material fully ferments, second of the reduction of heap temperature, material is loose, fermentation maturity, controls moisture, and screening crushes standby With.
(3) according to the percentage requirement of each beneficial bacterium, corresponding each part microbial inoculum is weighed, is obtained with above-mentioned steps b decomposed Good fermentation material mixing, stirs 3-5 minutes, is uniformly mixed.
(4) after mixing, humic acid is added, is again stirring for 3-5 minutes, is uniformly mixed and obtains finished product organic fertilizer.
(5) method of administration:Manufactured product organic fertilizer application holostrome deep placement, i.e., full field is spread fertilizer over the fields before transplanting, in conjunction with soil Turn over deep placement, also can ditch spread, spread manuer in holes, heap is applied, but it is noted that avoid fertilizer from directly being contacted with seed or crop root, to prevent from macerating Root.It is more preferable using effect is sprinkled profoundly water after this fertilizer.
Embodiment 2:Multifunctional biological fertilizer is made of the material of following weight percents:
Dregs of beans:7.5%, rapeseed meal:2.5%, humic acid:30%, vinasse:15%, chaff powder:15%, wheat bran:5%, sawdust Powder:7.5%, appropriate amount of water.
Microbial inoculum matches:Bacillus amyloliquefaciens (1.0%);Colloid bacillus cereus (0.25%);Paecilomyces lilacinus (0.3%).
Preparation method referring to embodiment 1 method.
Preparation method is as follows:
(1) preparation of fermenting microbe
Use front activating:By rich peasant No. 1 fermenting microbe 10g, water according to 1:50 ratio, activation 12 hours or so, during which Preferably it is sufficiently stirred 1-2 times.It is mixed with fermentation materials (material always weighs about 1 ton) after being diluted with water after activation.
(2) preparation of fermentation materials
A. by raw soybean dregs, rapeseed meal, vinasse, chaff powder, wheat bran, sawdust powder weighs by weight percentage, after rear addition activation Rich peasant's No. 1 fermenting microbe mixing, moisture control grabs the agglomerating no water droplet of material in 50%-60%, with hand, webs water breakthrough print but not It drips, landing, which can scatter, to be advisable, and during fermentation materials, whether moisture is suitable for very important.
b:Heap material heap is high
When doing pile fermentation heap, height is generally at 0.8-1.5 meters or so, and 2 meters or so of width, length is at 2~4 meters or more Heap ferment effect it is preferable.When 15 DEG C of environment temperature or less, covered with film or straw screen or mat, it, will when internal temperature is raised to 30 DEG C Covering is opened.Heap temperature is begun to tip over when rising to 60 DEG C, is turned over 2~3 times, if heap temperature is more than 65 DEG C, need to repeatedly be overturned, ordinary circumstance Under, it will appear 2~3 65 DEG C or more of high temperature during the fermentation.
When second of turning, cooling, inoculation fermentation strain, carries out second and ferments again, i.e., first by No. 1 hair of rich peasant Yeast-like fungi kind 10g is uniformly mixed with the rice bran (fermentation materials 1000KG of this example or so) of 2000g, strain mixture is made, then Again by strain mixture and fermentation materials mixed fermentation, material is made adequately to ferment.
After material fully ferments, second of the reduction of heap temperature, material is loose, fermentation maturity, controls moisture, and screening crushes standby With.
(3) according to the percentage requirement of each beneficial bacterium, corresponding each part microbial inoculum is weighed, is obtained with above-mentioned steps b decomposed Good fermentation material mixing, stirs 3-5 minutes, is uniformly mixed.
(4) after mixing, humic acid is added, is again stirring for 3-5 minutes, is uniformly mixed and obtains finished product organic fertilizer.
(5) method of administration:Manufactured product organic fertilizer application holostrome deep placement, i.e., full field is spread fertilizer over the fields before transplanting, in conjunction with soil Turn over deep placement, also can ditch spread, spread manuer in holes, heap is applied, but it is noted that avoid fertilizer from directly being contacted with seed or crop root, to prevent from macerating Root.
It is more preferable using effect is sprinkled profoundly water after this fertilizer.
The material formula (weight percent %) of 3 multifunctional biological fertilizer of embodiment
Material proportion:Dregs of beans:10%, rapeseed meal:2.5%, humic acid:30%, vinasse:10%, chaff powder:15%, wheat bran: 5%, sawdust powder:10%, appropriate amount of water.
Microbial inoculum matches:Bacillus amyloliquefaciens (1.3%);Colloid bacillus cereus (0.3%);Paecilomyces lilacinus (0.4%)
Preparation method and method of administration have method referring to embodiment 1 or 2.
Embodiment 4:Multifunctional biological fertilizer is made of the material of following weight percents:
Material formula:Dregs of beans:7.5%, rapeseed meal:5%, humic acid:30%, vinasse:10%, chaff powder:15%, wheat bran: 5%, sawdust powder:7.5%, appropriate amount of water.
Microbial inoculum matches:Bacillus amyloliquefaciens (2.0%);Colloid bacillus cereus (0.5%);Paecilomyces lilacinus (0.5%)
Preparation method and method of administration have method referring to embodiment 1 or 2.
Embodiment 5:The fertilizer efficiency of the multifunctional biological fertilizer of biological control root-knot nematode is tested
1) for trying fertilizer:A kind of multifunctional biological fertilizer with biological control root-knot nematode, sample are embodiment 1,2 The bio-organic fertilizer product prepared with 3.
2) for studying object:Cucumber (kind Yan Feng)
3) test method:Experiment arranges 5 processing, 3 repetitions, random district's groups arrangement.7.0 meters of zone leader, 4.0 meters wide, face 28 square metres of product.Aisle is 50 centimetres between processing, between repeating, and surrounding stays 1.0 meters of row of protection or more, selects the cucumber of ad eundem Cultivation.90 plants of cucumber, 70 × 50 centimetres of specification are planted per cell, folding mu plants 2142 plants.Cultivation management other than process content is arranged It applies and implements according to locality production, and control consistent.It is completed on the same day per the identical measure of cell.Each process content is as follows:
Processing 1:3 biological organic fertilizers of embodiment+conventional fertilizer application decrement 20%:200 kilograms of biological organic fertilizer of mu before final singling, It is turned over after 16 kilograms of 16.0 kilograms+dung polluted water of Diammonium phosphate (DAP), 400.0 kilograms+potassium sulfate, it is additional after final singling to apply 8 kgs/acre of urea, The phase of yielding positive results applies 8 kgs/acre of urea, 12 kgs/acre of calcium superphosphate, and 8 kgs/acre of potassium chloride respectively imposes once May June.
Processing 2:2 biological organic fertilizers of embodiment+conventional fertilizer application decrement 20%:200 kilograms of biological organic fertilizer of mu before final singling, It is turned over after 16 kilograms of 16.0 kilograms+dung polluted water of Diammonium phosphate (DAP), 400.0 kilograms+potassium sulfate, it is additional after final singling to apply 8 kgs/acre of urea, The phase of yielding positive results applies 8 kgs/acre of urea, 12 kgs/acre of calcium superphosphate, and 8 kgs/acre of potassium chloride respectively imposes once May June.
Processing 3:1 biological organic fertilizer of embodiment+conventional fertilizer application decrement 20%:200 kilograms of biological organic fertilizer of mu before final singling, It is turned over after 16 kilograms of 16.0 kilograms+dung polluted water of Diammonium phosphate (DAP), 400.0 kilograms+potassium sulfate, it is additional after final singling to apply 8 kgs/acre of urea, The phase of yielding positive results applies 8 kgs/acre of urea, 12 kgs/acre of calcium superphosphate, and 8 kgs/acre of potassium chloride respectively imposes once May June.
Processing 4:Conventional fertilizer application:Mu is public with 20.0 kilograms+dung polluted water of Diammonium phosphate (DAP), 500.0 kilograms+potassium sulfate 20 before final singling It is turned over after jin, additional after final singling to apply 10 kgs/acre of urea, the phase of yielding positive results applies 10 kgs/acre of urea, 15 kilograms of calcium superphosphate/ Mu, 10 kgs/acre of potassium chloride respectively impose once May June;
Processing 5:Blank control (not applying any fertilizer) is poured with clear water.
Cucumber is colonized March 13, April 21 starts to harvest, and June 26, harvesting terminated, and single meter yield is received per cell list.
4) the above cucumber yield respectively handled and the influence to per mu yield are calculated
Unit:Kilogram
The per mu yield of 1 embodiment 1-3 organic fertilizers of table and conventional fertilizer application and blank control
It is comparison result of each processing to cucumber yield with upper table 1.
Be below embodiment 1-3 organic fertilizers and conventional fertilizer application and blank control (handling 1-5) per mu yield and Relative growth rate see the table below 2, unit:Kilogram, yield:%.
The opposite rate of growth of 2 embodiment 1-3 organic fertilizers of table and conventional fertilizer application and blank control
Cucumber applies example 3,2,1 biological organic fertilizer+conventional fertilizer application decrement 20%, and paid per mu yield is respectively 3718 public affairs Jin, 3614 kilograms, 3547 kilograms, compare conventional fertilizer application, mu volume increase be respectively 462 kilograms, 358 kilograms, 291 kilograms;It is corresponding Rate of growth is respectively 14%, 11%, 9%.
And the organic fertilizer obtained by the embodiment 1 of CN105859377, carry out experiment per mu yield by the method for above-mentioned processing 1 3345kg。
Embodiment 5:The effect experiment of the multifunctional biological fertilizer of biological control root-knot nematode
5 processing are arranged, 7.0 meters of zone leader is 4.0 meters wide, 28 square metres of area.Aisle is 50 centimetres between processing, and four It stays 1.0 meters of row of protection or more week, selects the cucumber cultivation of ad eundem.90 plants of cucumber is planted per cell, 70 × 50 centimetres of specification connects Kind 2000/plant of root-knot nematode egg.5 processing, which wherein handle the biology that 1,2,3 pair of application sample is the preparation of embodiment 1,2,3, to be had Machine fertilizer product, processing 4 are the organic fertilizer (positive control) obtained by the embodiment 1 of CN105859377, and processing 5 is blank control It is replaced with clear water.
Then root knot line before treatment and around the field cucumber root of treated fruiting period randomly selects each processing (1-5) Worm, investigation nematode population (item/50 gram soil), and the root-knot nematode slip that 1-4 is handled relative to blank control fruiting period is calculated, It the results are shown in Table 3.
Table 3 respectively handles root-knot nematode sample investigation and nematode slip around the cucumber root of field
Processing Before processing Fruiting period Root nematode slip
Processing 1 190 59 60.7%*
Processing 2 188 46 69.3%
Processing 3 192 22 85.3%
Handle 4 (positive controls) 191 71 52.7%
Handle 5 (blank controls) 189 150 ---
* root nematode slip=((blank control fruiting period quantity-respectively handles fruiting period quantity)/blank control fruiting period quantity) X100%.
Cucumber apply 1,2,3 biological organic fertilizer of example handle with blank control in fruiting period compared with, root-knot nematode slip Respectively 60.7%, 69.3%, 85.3%, and the root-knot nematode slip of positive control processing group is 52.7%.Embodiment 4 The root knot line slip of biological organic fertilizer is suitable with embodiment 1.
Above-mentioned Tables 1 and 2 statistics indicate that, the biological organic fertilizer of the bright present invention not only effectively controls root-knot nematode, and Also there is remarkable result in terms of improving crop yield.
Embodiments of the invention described above be only it is representative, it is any in the case that without departing from the present invention Spirit Essence appoint What modification or simple replace also belong to the scope of the present invention.

Claims (10)

1. a kind of multifunctional biological fertilizer of prevention root-knot nematode, is mainly made of following material and microbial inoculum, the material packet Include the raw material of following weight percent:Dregs of beans 5-10%, rapeseed meal 2.5-5%, humic acid 25%-35%, vinasse 5-15%, chaff powder 15-30%, wheat bran 5-10%, sawdust powder 5-10% and moisture 10-25%, the microbial inoculum be bacillus amyloliquefaciens 0.5-2.0%, Colloid bacillus cereus 0.1-0.5%, Paecilomyces lilacinus 0.1-0.5%.
2. biological organic fertilizer as described in claim 1, the material includes dregs of beans 5%-10%, rapeseed meal 2.5-5%, humic acid 25%-30%, vinasse 10-15%, chaff powder 15-20%, wheat bran 5-10%, sawdust powder 5-10% and moisture 15-20%.
3. biological organic fertilizer as claimed in claim 1 or 2, the material, wherein the carbon content that can directly utilize is 8-20%, The nitrogen quantity that can directly utilize is 0.3-1.2%.
4. biological organic fertilizer as claimed in claim 1 or 2, the microbial inoculum, including bacillus amyloliquefaciens 1.0-1.5%, glue Matter bacillus 0.2-0.3% and Paecilomyces lilacinus 0.25-0.4%.
5. a kind of method for the biological organic fertilizer preparing claim 1, includes the following steps:
(1) activation of fermenting microbe
By No. 1 fermenting microbe of rich peasant and water according to 1:50 weight ratio mixing, activation 12 hours or so is to get activation leavening; Period is preferably sufficiently stirred 1-2 times, is mixed with fermentation materials after the dilution that can be added to the water later;
(2) preparation of fermentation materials
A) by raw soybean dregs, rapeseed meal, vinasse, chaff powder, wheat bran, after mixing, the activation bacterium that step (1) obtains is added in sawdust powder Agent, is added water after mixing, moisture is controlled in 50%-60%;
B) windrow:Upper step is mixed with microbial inoculum material close after pile fermentation heap, the height of the fermentation heap is a 0.8-1.5 meter left sides The right side, width are 2 meters or so, and length, by fermentation heap covering and heat insulating, waits for inside at 2~4 meters or more, 15 DEG C of environment temperature or less When temperature is raised to 30 DEG C, covering is opened, heap temperature is begun to tip over when rising to 60 DEG C;
C) when overturning for second, or it can be inoculated with No. 1 fermenting microbe secondary fermentation of rich peasant again, material is made fully to ferment;
D) heap temperature drop is low, and material is loose, after fermentation maturity, controls moisture, it is spare that screening crushes post-processing;
(3) microbial inoculum is added in decomposed good fermentation materials, is mixed;3-5 minutes;
(4) after mixing, humic acid is added, mixes 3-5 minutes obtain organic fertilizer finished product again.
6. method as claimed in claim 5, the secondary fermentation of the step c) further comprise chaff powder or wheat bran and richness first No. 1 fermenting microbe of agriculture is uniformly mixed, and strain mixture is made, is then with strain mixture and fermentation materials mixed fermentation again It can, wherein the weight ratio of chaff powder or wheat bran and No. 1 fermenting microbe of rich peasant is 100-200:1.
7. method as claimed in claim 5, No. 1 fermenting microbe of the rich peasant includes bacillus subtilis and saccharomyces cerevisiae, Dosage is that every 1 ton of material adds No. 1 fermenting microbe of 5-15g rich peasant, preferably 10g.
8. method as claimed in claim 5 controls moisture described in step a), hand is made to grab the agglomerating no water droplet of material, webs are shown in It watermark but does not drip, landing can scatter.
9. method as claimed in claim 5, the environment temperature of fermentation heap is at 15 DEG C or more.
10. method as claimed in claim 5, in step b), when heap temperature is more than 65 DEG C, should repeatedly overturn.
CN201810888495.4A 2018-08-07 2018-08-07 A kind of multifunctional biological fertilizer and preparation method thereof of prevention root-knot nematode Pending CN108727124A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110540439A (en) * 2019-08-16 2019-12-06 广西大学 Biological bacterial fertilizer for preventing and treating sugarcane smut and preparation method thereof
CN111517864A (en) * 2020-05-26 2020-08-11 江西三品生物科技有限公司 Special biological organic fertilizer for preventing and treating nematodes and preparation method thereof
CN112314639A (en) * 2020-11-03 2021-02-05 广东省农业科学院茶叶研究所 Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof

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CN101165016A (en) * 2007-10-12 2008-04-23 马玉新 Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof
CN107619308A (en) * 2016-07-13 2018-01-23 湖南泰谷生物科技股份有限公司 Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application
CN108017450A (en) * 2017-12-26 2018-05-11 广西多得乐生物科技有限公司 Special anti-nematode composite microbiological fertilizer of one planting fruit-trees and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN101165016A (en) * 2007-10-12 2008-04-23 马玉新 Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof
CN107619308A (en) * 2016-07-13 2018-01-23 湖南泰谷生物科技股份有限公司 Prevent and treat root-knot nematode biological organic fertilizer and preparation method and application
CN108017450A (en) * 2017-12-26 2018-05-11 广西多得乐生物科技有限公司 Special anti-nematode composite microbiological fertilizer of one planting fruit-trees and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110540439A (en) * 2019-08-16 2019-12-06 广西大学 Biological bacterial fertilizer for preventing and treating sugarcane smut and preparation method thereof
CN111517864A (en) * 2020-05-26 2020-08-11 江西三品生物科技有限公司 Special biological organic fertilizer for preventing and treating nematodes and preparation method thereof
CN112314639A (en) * 2020-11-03 2021-02-05 广东省农业科学院茶叶研究所 Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof

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