CN108976065A - A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode - Google Patents
A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode Download PDFInfo
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- CN108976065A CN108976065A CN201810834333.2A CN201810834333A CN108976065A CN 108976065 A CN108976065 A CN 108976065A CN 201810834333 A CN201810834333 A CN 201810834333A CN 108976065 A CN108976065 A CN 108976065A
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- Prior art keywords
- compound fertilizer
- biological compound
- fermentation liquid
- knot nematode
- decomposed
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a kind of biological compound fertilizers and its processing method for preventing and treating root-knot nematode, the biological compound fertilizer of the prevention and treatment root-knot nematode, the biological compound fertilizer includes organic matter 60~85%, nematode biocontrol fungi fermentation liquid 8~20%, bacillus amyloliquefaciens fermentation liquid 8~20%.In the present invention on the one hand decomposed organic matter can increase soil fertility as organic fertilizer, improve soil texture, provide nutrition for plant growth, increase yield, improving quality；On the other hand, organic fertilizer can be Paecilomyces lilacinus strain breeding and the required nutrition that plays a role, and significantly improve it in the colonization amount of plant rhizosphere, preferably play the effect of prevention and treatment root knot nematode disease.
Description
Technical field
The present invention relates to technical field of biological fertilizer more particularly to a kind of biological compound fertilizer for preventing and treating root-knot nematode and its add
Work method.
Background technique
In recent years, there is the trend aggravated year by year in facility cultivation in root-knot nematode harm.Root-knot nematode
(Meloidogyne, spp) is a kind of omnivorousness plant pathogeny line insect of height specialized form, vegetable root portion of mainly causing harm, to kind
The important vegetables production such as eggplant, cucumber, capsicum causes damages, and can lead to the production underproduction 20%~70%；The harm of root-knot nematode is also
It can aggravate the generation of the soil fungal disease such as wilt disease, root rot, it is extremely difficult to cure.And soil-fumigating is carried out using chemical agent
Although prevention and treatment root-knot nematode have the effect of it is certain but at high cost, to ecological environment of soil negatively affect it is larger, therefore application give birth to
The harm that object means of prevention prevents and treats root-knot nematode becomes research hotspot.
Paecilomyces lilacinus (Paecilomyces lilacinus (Thorn.) Samson) is a kind of endoparasitism fungi, is
The Important Natural Enemy of some plant nematodes can parasitize ovum, can also infect larva and female adult, can substantially reduced various crop
The harm of root knot nematode disease is presently considered to be most promising nematode biocontrol fungi.Related biocontrol agent existing at present
It is used in production.In practical applications, the effect that often will appear root knot nematode disease evil biological control is unstable, this may be with
Biocontrol microorganisms adaptability deficiency under the ecological environment of crop field complexity is related.
Biological compound fertilizer is a kind of fertilizer for being inoculated with beneficial microbe on the basis of traditional organic fertilizer and producing.Biology
Compound fertilizer both can increase organic matter for soil, improve soil texture, promote plant growth, wherein the beneficial microbe contained is also
It can inhibit soil harmful microorganism, soil ecosystem is promoted to restore, enhance disease resistance of plant.
In view of the foregoing, a kind of bio-feritlizer by biocontrol microorganisms compounding into decomposed organic matter is proposed.
Summary of the invention
The main purpose of the present invention is to provide a kind of biological compound fertilizers for preventing and treating root-knot nematode, it is desirable to provide a kind of to give birth to
Fungi-proofing bio-feritlizer of the compounding into decomposed organic matter promotes biocontrol microorganisms colonizing in the soil, gives full play to biological and ecological methods to prevent plant disease, pests, and erosion characteristic.
To achieve the above object, a kind of biological compound fertilizer for preventing and treating root-knot nematode provided by the invention, the biology are compound
Fertilizer includes organic matter 60~85%, nematode biocontrol fungi fermentation liquid 8~20%, bacillus amyloliquefaciens fermentation liquid 8~20%.
Preferably, the nematode biocontrol fungi fermentation liquid is Paecilomyces lilacinus fermentation liquid.
Preferably, the biological compound fertilizer includes organic matter 70~80%, Paecilomyces lilacinus fermentation liquid 10~15%, solution
Bacillus amyloliquefaciens fermentation liquid 10~15%.
Preferably, the organic matter includes decomposed edible fungi residue 20~30%, decomposed manioc waste 20~30%, decomposed wood
Sugar residue 20~30%.
Preferably, the biological compound fertilizer includes decomposed edible fungi residue 22%, decomposed manioc waste 24%, decomposed xylose residue
23%, Paecilomyces lilacinus fermentation liquid 12%, bacillus amyloliquefaciens fermentation liquid 12%.
In addition, to achieve the above object, the present invention also proposes a kind of processing side of biological compound fertilizer for preventing and treating root-knot nematode
Method, the processing method are used to produce the biological compound fertilizer of prevention and treatment root-knot nematode as described above, and the processing method includes such as
Lower step:
Step S10 selects organic matter material fresh, without mildew, adjusts C/N ratio；
Step S20, decomposing agent fermentation, which is added, keeps organic matter completely decomposed；
Step S30 ferments Paecilomyces lilacinus and bacillus amyloliquefaciens using seeding tank respectively；
Paecilomyces lilacinus and bacillus amyloliquefaciens fermentation liquid are uniformly mixed by step S40 with decomposed organic matter；
Step S50, by uniformly mixed Paecilomyces lilacinus, bacillus amyloliquefaciens fermentation liquid and decomposed organic matter low
It is dried, is granulated at a temperature of 40 DEG C, obtain product.
Preferably, in the step S30, effective spore concentration >=3 × 10 in Paecilomyces lilacinus fermentation liquid after fermentation8
A/ml, gemma and viable count >=1 × 10 in bacillus amyloliquefaciens fermentation liquid9A/ml.
Preferably, in the biological compound fertilizer that the processing method obtains: Paecilomyces lilacinus viable count >=1 × 108CFU/g,
Bacillus amyloliquefaciens gemma and viable count >=1 × 108CFU/g。
Preferably, in the step S10, adjustment C/N ratio is 25:1.
The biological compound fertilizer of prevention and treatment root-knot nematode of the invention, the biological compound fertilizer includes organic matter 60~85%, line
Worm biocontrol fungi fermentation liquid 8~20%, bacillus amyloliquefaciens fermentation liquid 8~20%.Decomposed organic matter is used as and has in the present invention
On the one hand machine fertilizer can increase soil fertility, improve soil texture, provide nutrition for plant growth, increase yield, improving quality；
On the other hand, organic fertilizer can be Paecilomyces lilacinus strain breeding and the required nutrition that plays a role, and significantly improve it in plant
The colonization amount of rhizosphere preferably plays the effect of prevention and treatment root knot nematode disease.
Specific embodiment
It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not intended to limit the present invention.
The present invention provide it is a kind of prevent and treat root-knot nematode biological compound fertilizer, the biological compound fertilizer include organic matter 60~
85%, nematode biocontrol fungi fermentation liquid 8~20%, bacillus amyloliquefaciens fermentation liquid 8~20%.
Preferably, the nematode biocontrol fungi fermentation liquid is Paecilomyces lilacinus fermentation liquid.
Preferably, the biological compound fertilizer includes organic matter 70~80%, Paecilomyces lilacinus fermentation liquid 10~15%, solution
Bacillus amyloliquefaciens fermentation liquid 10~15%.
Preferably, the organic matter include decomposed edible fungi residue 20~30%, it is decomposed manioc waste 20~30%, decomposed
Xylose residue 20~30%.
Preferably, the biological compound fertilizer includes decomposed edible fungi residue 22%, decomposed manioc waste 24%, decomposed xylose residue
23%, Paecilomyces lilacinus fermentation liquid 12%, bacillus amyloliquefaciens fermentation liquid 12%.
In addition, to achieve the above object, the present invention also proposes a kind of processing side of biological compound fertilizer for preventing and treating root-knot nematode
Method, the processing method are used to produce the biological compound fertilizer of prevention and treatment root-knot nematode as described above, and the processing method includes such as
Lower step:
Step S10 selects organic matter material fresh, without mildew, adjusts C/N ratio；
Step S20, decomposing agent fermentation, which is added, keeps organic matter completely decomposed；
Step S30 ferments Paecilomyces lilacinus and bacillus amyloliquefaciens using seeding tank respectively；
Paecilomyces lilacinus and bacillus amyloliquefaciens fermentation liquid are uniformly mixed by step S40 with decomposed organic matter；
Step S50, by uniformly mixed Paecilomyces lilacinus, bacillus amyloliquefaciens fermentation liquid and decomposed organic matter low
It is dried, is granulated at a temperature of 40 DEG C, obtain product.
Preferably, in the step S30, effective spore concentration >=3 × 10 in Paecilomyces lilacinus fermentation liquid after fermentation8
A/ml, gemma and viable count >=1 × 10 in bacillus amyloliquefaciens fermentation liquid9A/ml.
Preferably, in the biological compound fertilizer that the processing method obtains: Paecilomyces lilacinus viable count >=1 × 108CFU/g,
Bacillus amyloliquefaciens gemma and viable count >=1 × 108CFU/g。
Preferably, in the step S10, adjustment C/N ratio is 25:1.
Specifically, organic matter material fresh, without mildew is selected, adjustment C/N ratio is 25:1, using commercially available high-quality decomposing agent
Fermentation maturity is complete.Paecilomyces lilacinus and bacillus amyloliquefaciens are fermented respectively using seeding tank, after fermentation pale purple quasi- blueness
Effective spore concentration >=3 × 10 in mould fermentation liquid8A/ml, gemma and viable count >=1 × 10 in bacillus amyloliquefaciens fermentation liquid9
A/ml.Paecilomyces lilacinus and bacillus amyloliquefaciens fermentation liquid are uniformly mixed with decomposed organic matter, low temperature drying, granulation (temperature
Degree is lower than 40 DEG C), final products Paecilomyces lilacinus viable count >=1 × 108CFU/g, bacillus amyloliquefaciens gemma and viable count
≥ 1×108CFU/g。
The novel strain that Paecilomyces lilacinus system independently separates contained in biological compound fertilizer of the present invention.It is light
Purple Paecilomyces varioti bacterial strain colonizes the quantity of nematode in effectively control soil in plant rhizosphere, reduces harm.The bacterial strain and energy simultaneously
Promote the growth of plant nutrition organ, increases crop yield.
The novel bacteria that bacillus amyloliquefaciens system independently separates contained in biological compound fertilizer of the present invention
Strain, the bacterial strain carry out preservation, preservation in China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC
Number: ACCC04304, preservation time: on 08 23rd, 2013；Preservation address: China Committee for Culture Collection of Microorganisms is general
Logical microorganism center.
In production, root-knot nematode harm is often adjoint or aggravates plant disease.Bacillus amyloliquefaciens, it is anti-with wide spectrum
Bacterium activity, can inhibit plurality of plant diseases.
Without antagonism between bacillus amyloliquefaciens and Paecilomyces lilacinus and two kinds of bacterial strains.Bacillus amyloliquefaciens with it is light
Purple Paecilomyces varioti compounding, effect in unison can preferably inhibit the plant pathogenic microorganisms associated with root-knot nematode, mitigate danger
Evil makes plant not fall ill or mitigate to fall ill and endangers.In addition, the various active substance that bacillus amyloliquefaciens metabolism generates,
It can promote plant growth, improve quality of agricultural product.
Paecilomyces lilacinus and bacillus amyloliquefaciens are lighter plant rhizosphere acquirements of falling ill from different root-knot nematode regions of disease
Pedotheque in the antagonistic strain that filters out, have the characteristics that fast growth, resistance, passage are stable.Two kinds of bacterial strains be from
It is directly separated in soil, inoculating in soil has the characteristics that colonazition is strong, growth is rapid, resistance is strong, overcomes part
In the excellent bacterial strain of the laboratory performance often disadvantage bad in variable rate technology.
It is its important prerequisite for playing biological and ecological methods to prevent plant disease, pests, and erosion effectiveness that biocontrol microorganisms colonize in the soil.When the conditions such as temperature, humidity, nutrition are suitable
It just can guarantee and colonize when preferably.In the present invention on the one hand decomposed organic matter can increase soil fertility as organic fertilizer, improve
Soil texture provides nutrition for plant growth, increases yield, improving quality；On the other hand, organic fertilizer can be Paecilomyces lilacinus
Bacterial strain breeding and the required nutrition that plays a role significantly improve it in the colonization amount of plant rhizosphere, preferably play prevention and treatment root knot
The effect of nematodiasis.
Under the cooperation of above-mentioned each factor, the effect of comparatively ideal prevention and treatment root knot nematode disease can be obtained after present invention fertilizer application
Fruit, and can be used for a long time, while yield can be increased, improve quality of agricultural product.
Biological compound fertilizer dosage of the present invention are as follows: crop field, thousand grams/acre of 50-100；Protecting field, thousand grams/acre of 80-150.
Specific method of application are as follows: as base manure in sowing or disposable application in field planting first 10~15 days.
The present invention is suitable for the multiple kinds of crops such as vegetables, industrial crops.
Embodiment 1
Control efficiency of the present invention to tomato root-knot eelworm disease.The common vegetable for experimental field thering is root-knot nematode to endanger for upper one batch
Dish greenhouse applies the present invention before field planting, to apply common organic fertilizer as control, repeats three times.Tomato variety is that gingko is rich by force,
Line-spacing 35cm, spacing in the rows 30cm, the high 15cm in ridge, Routine Management.As a result it as shown in table 1,2, can be significantly reduced after applying this product
The harm of root-knot nematode enhances tomato disease resistance, moreover it is possible to improve fruit quality, increase the economic value of tamato fruit.
Influence of the present invention of table 1 to tomato yield, picking time and its to the inhibiting effect of root-knot nematode
Influence of 2 present invention of table to Quality of Tomato Fruit
Embodiment 2
Control efficiency of the present invention to Cucumber root-knot nematode disease.Cucumber experimental cultivar is the saliva spring No. 3, using high ridge ridge culture,
Wide-and narrow-row cultivation, plot area 10m2, measurement early yield (are compareed up to the yield in 15d after the beginning receipts phase), and every cell takes 5 plants
Observe the increment in its 15d.As a result as shown in Table 3, 4, the harm of root knot nematode disease can effectively be mitigated after application this product,
Increase yield, improves fruit quality.
3 present invention of table is to cucumber growth, yield and its to the control efficiency of root-knot nematode
Influence of 4 present invention of table to cucumber quality
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair
Equivalent structure or equivalent flow shift made by bright description is applied directly or indirectly in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (9)
1. it is a kind of prevent and treat root-knot nematode biological compound fertilizer, which is characterized in that the biological compound fertilizer include organic matter 60~
85%, nematode biocontrol fungi fermentation liquid 8~20%, bacillus amyloliquefaciens fermentation liquid 8~20%.
2. the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 1, which is characterized in that the nematode biocontrol fungi
Fermentation liquid is Paecilomyces lilacinus fermentation liquid.
3. the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 2, which is characterized in that the biological compound fertilizer packet
Include organic matter 70~80%, Paecilomyces lilacinus fermentation liquid 10~15%, bacillus amyloliquefaciens fermentation liquid 10~15%.
4. the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 2, which is characterized in that the organic matter includes corruption
Ripe edible fungi residue 20~30%, decomposed manioc waste 20~30%, decomposed xylose residue 20~30%.
5. the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 4, which is characterized in that the biological compound fertilizer packet
Include decomposed edible fungi residue 22%, decomposed manioc waste 24%, decomposed xylose residue 23%, Paecilomyces lilacinus fermentation liquid 12%, Xie Dian
Afnyloliquefaciens fermentation liquid 12%.
6. a kind of processing method for the biological compound fertilizer for producing prevention and treatment root-knot nematode as claimed in any one of claims 1 to 5,
It is characterized in that, the processing method includes the following steps:
Step S10 selects organic matter material fresh, without mildew, adjusts C/N ratio；
Step S20, decomposing agent fermentation, which is added, keeps organic matter completely decomposed；
Step S30 ferments Paecilomyces lilacinus and bacillus amyloliquefaciens using seeding tank respectively；
Paecilomyces lilacinus and bacillus amyloliquefaciens fermentation liquid are uniformly mixed by step S40 with decomposed organic matter；
Uniformly mixed Paecilomyces lilacinus, bacillus amyloliquefaciens fermentation liquid and decomposed organic matter are being lower than 40 by step S50
It is dried, is granulated at a temperature of DEG C, obtain product.
7. the processing method of the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 6, which is characterized in that the step
In rapid S30, effective spore concentration >=3 × 10 in Paecilomyces lilacinus fermentation liquid after fermentation8A/ml, bacillus amyloliquefaciens
Gemma and viable count >=1 × 10 in fermentation liquid9A/ml.
8. the processing method of the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 6, which is characterized in that described to add
In the biological compound fertilizer that work method obtains: Paecilomyces lilacinus viable count >=1 × 108CFU/g, bacillus amyloliquefaciens gemma and work
Bacterium number >=1 × 108CFU/g。
9. the processing method of the biological compound fertilizer of prevention and treatment root-knot nematode according to claim 6, which is characterized in that the step
In rapid S10, adjustment C/N ratio is 25:1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810834333.2A CN108976065A (en) | 2018-07-26 | 2018-07-26 | A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode |
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| CN201810834333.2A CN108976065A (en) | 2018-07-26 | 2018-07-26 | A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode |
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Cited By (7)
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| CN109832287A (en) * | 2019-03-21 | 2019-06-04 | 四川省农业科学院生物技术核技术研究所 | A kind of preparation and preparation method thereof improving prevention and treatment root nematode bio-pharmaceutical drug effect |
| CN110668903A (en) * | 2019-10-23 | 2020-01-10 | 中国农业科学院烟草研究所 | Microbial fertilizer for comprehensive treatment of nematode |
| CN110698276A (en) * | 2019-10-23 | 2020-01-17 | 中国农业科学院烟草研究所 | Microbial fertilizer for preventing and treating nematodes |
| CN111362760A (en) * | 2020-03-09 | 2020-07-03 | 沃博特生物科技有限公司 | Compound microbial agent-coated biological compound fertilizer and preparation method thereof |
| CN112314639A (en) * | 2020-11-03 | 2021-02-05 | 广东省农业科学院茶叶研究所 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
| CN114455985A (en) * | 2022-02-14 | 2022-05-10 | 云南省农业科学院农业环境资源研究所 | Preparation method of compound biological agent for preventing and treating aucklandia root rot and nematode disease |
| CN114711069A (en) * | 2022-04-11 | 2022-07-08 | 广西南亚热带农业科学研究所 | Comprehensive prevention and control method for pitaya root-knot nematodes |
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| CN112314639A (en) * | 2020-11-03 | 2021-02-05 | 广东省农业科学院茶叶研究所 | Biological pesticide fertilizer for preventing and treating tea seedling root knot nematode disease and preparation method thereof |
| CN114455985A (en) * | 2022-02-14 | 2022-05-10 | 云南省农业科学院农业环境资源研究所 | Preparation method of compound biological agent for preventing and treating aucklandia root rot and nematode disease |
| CN114711069A (en) * | 2022-04-11 | 2022-07-08 | 广西南亚热带农业科学研究所 | Comprehensive prevention and control method for pitaya root-knot nematodes |
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