CN110698276A - Microbial fertilizer for preventing and treating nematodes - Google Patents

Microbial fertilizer for preventing and treating nematodes Download PDF

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Publication number
CN110698276A
CN110698276A CN201911009446.XA CN201911009446A CN110698276A CN 110698276 A CN110698276 A CN 110698276A CN 201911009446 A CN201911009446 A CN 201911009446A CN 110698276 A CN110698276 A CN 110698276A
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China
Prior art keywords
microbial
base layer
microbial fertilizer
substrate
fermentation
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Pending
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CN201911009446.XA
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Chinese (zh)
Inventor
王杰
王凤龙
王静
杨金广
申莉莉
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Tobacco Research Institute of CAAS
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Tobacco Research Institute of CAAS
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Priority to CN201911009446.XA priority Critical patent/CN110698276A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to a microbial fertilizer for preventing and treating nematodes, which comprises the following components: the fermentation substrate, a first microbial strain, a second microbial strain, a first base layer and a second base layer; the fermentation substrate is fermented in a fermentation tank to form humus; performing activation culture on the first microbial strain and the second microbial strain to form a first culture solution and a second culture solution, and soaking the first substrate and the second substrate in the first culture solution and the second culture solution respectively; at least one side of the first base layer and the second base layer is coated with a fermentation substrate, and the first base layer and the second base layer are compressed to form a layered body; the surface body covers the soil surface or is buried in a shallow layer of the soil, the fermentation substrate on the surface body is mixed with microbial strains for slow-release degradation, and the surface body can be used for nematode prevention and control.

Description

Microbial fertilizer for preventing and treating nematodes
Technical Field
The invention relates to a microbial fertilizer, in particular to a microbial fertilizer for preventing and treating nematodes.
Background
The organic fertilizer is mainly organic substances formed by taking agricultural wastes, livestock and poultry manure, industrial wastes, household garbage and the like as fertilizers and performing microbial fermentation decomposition and conversion, the microbial fertilizer is formed by adding part of microbial strains into the organic fertilizer, so that the fertilizer can promote the growth of plant root systems, has the capability of microbial degradation and can simultaneously prevent and control plant diseases and insect pests and nematodes, and the root-knot nematodes are highly obligate parasitic plant pathogenic nematodes and mainly damage the roots of plants, mainly show that lateral roots and fibrous roots are more normally increased, the overground parts of damaged plants are short and small in growth, slow in growth and abnormal in leaf color, so that the yield is low, and even the plants die in advance.
At present, most of traditional nematode control methods adopt medicines for killing, and the other method adopts the bacillus subtilis and other medicines for matched control, so that the nematode control method cannot achieve long-term effective radical control, cannot achieve comprehensive control in the existing methods, cannot achieve control of root-knot nematodes in the fertilization process of plants, and cannot achieve comprehensive control of nutrient supply and pest control in growth of the plants.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides the microbial fertilizer for preventing and treating the nematodes.
In order to achieve the purpose, the invention adopts a technical scheme that: a microbial fertilizer for controlling nematodes, comprising: the fermentation substrate, a first microbial strain, a second microbial strain, a first base layer and a second base layer; the fermentation substrate is fermented in a fermentation tank to form humus; performing activation culture on the first microbial strain and the second microbial strain to form a first culture solution and a second culture solution, and soaking the first substrate and the second substrate in the first culture solution and the second culture solution respectively; at least one side of the first base layer and the second base layer is coated with a fermentation substrate, and the first base layer and the second base layer are compressed to form a layered body; the surface body covers the soil surface or is buried in a shallow layer of the soil, the fermentation substrate on the surface body is mixed with microbial strains for slow-release degradation, and the surface body can be used for nematode prevention and control.
In a preferred embodiment of the present invention, the first microorganism strain comprises at least one of Bacillus subtilis, Paecilomyces lilacinus, Pythium oligandrum, Trichoderma viride, Bacillus pumilus, Bacillus amyloliquefaciens and Pseudomonas defensins.
In a preferred embodiment of the present invention, the second microorganism strain comprises at least one of Bacillus subtilis, Paecilomyces lilacinus, Pythium oligandrum, Trichoderma viride, Bacillus pumilus, Bacillus amyloliquefaciens and Pseudomonas defensins.
In a preferred embodiment of the invention, anaerobic fermentation biological bacteria are added into the fermentation tank.
In a preferred embodiment of the invention, the fermentation substrate comprises one of cassava starch or pepper cake or pepper seed powder.
In a preferred embodiment of the invention, the fermentation substrate comprises one of cassava starch or pepper cake or pepper seed powder.
In a preferred embodiment of the present invention, the culture medium used in the culturing of the first and second microbial species is cultured according to standard fungal culture media recipes.
In a preferred embodiment of the present invention, the pH value of the laminar body is 7.2-8.3.
In a preferred embodiment of the invention, the carbon-nitrogen ratio of the microbial fertilizer on the laminar body is 24-26: 1.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) cassava starch is selected as the fermentation substrate, indole can be generated after the cassava starch is fermented, the growth of plants can be promoted, the yield can be increased, humus is formed after the fermentation substrate is fermented and is mixed with microbial strains to form a microbial fertilizer, the fertilizer has microbial degradation capability, the prevention and the treatment of nematodes can be carried out, and the effect and the function are more diversified.
(2) Microbial strains and humus are coated on a base layer through a layer structure to form a layer body, and the layer body is laid on the surface of soil or buried in the soil for nematode control, so that pesticide effect and nutrient are slowly released, and long-time nematode control is realized.
(3) The microbial strains slowly permeate into the soil, the degradation effect of the microbial strains and the prevention and control effect of nematodes can be improved, and meanwhile, the microbial strains are soaked in the base layer and can be adhered to the base layer in high concentration, so that the activity of the microbial strains is improved.
(4) The microbial strains are attached to the surface of the base layer in a soaking mode, so that the microbial strains can be uniformly and firmly attached to the surface of the base layer, the microbial strains are prevented from being seriously lost, and meanwhile, the base layer is made of degradable materials, so that the microbial strains can be prevented and treated from nematode, and the soil pollution can not be caused.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic partial structure view of a preferred embodiment of the present invention;
in the figure: 1. the biological fertilizer comprises a first base layer, 2, humus, 3, a second base layer, 4, a second microbial strain, 5 and a first microbial strain.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
As shown in fig. 1, a microbial fertilizer for controlling nematodes includes: the fermentation substrate, a first microbial strain, a second microbial strain, a first base layer and a second base layer; the fermentation substrate is fermented in a fermentation tank to form humus; performing activation culture on the first microbial strain and the second microbial strain to form a first culture solution and a second culture solution, and soaking the first substrate and the second substrate in the first culture solution and the second culture solution respectively; at least one side of the first base layer and the second base layer is coated with a fermentation substrate, and the first base layer and the second base layer are compressed to form a layered body; the surface body covers the soil surface or is buried in a shallow layer of the soil, the fermentation substrate on the surface body is mixed with microbial strains for slow-release degradation, and the surface body can be used for nematode prevention and control.
Microbial strains and humus are coated on a base layer through a layer structure to form a layer body, and the layer body is laid on the surface of soil or buried in the soil for nematode control, so that pesticide effect and nutrient are slowly released, and long-time nematode control is realized.
The first microorganism strain comprises at least one of bacillus subtilis, paecilomyces lilacinus, pythium oligandrum, trichoderma viride, bacillus pumilus, bacillus amyloliquefaciens and pseudomonad defender, the second microorganism strain comprises at least one of bacillus subtilis, paecilomyces lilacinus, pythium oligandrum, trichoderma viride, bacillus pumilus, bacillus amyloliquefaciens and pseudomonad defender, anaerobic fermentation biological bacteria are added into the fermentation tank, the fermentation substrate comprises one of cassava starch, pepper cake or pepper seed powder, and the fermentation substrate comprises one of cassava starch, pepper cake or pepper seed powder.
Cassava starch is selected as a fermentation substrate, tryptophan is contained in cassava, indole is formed after decomposition of bacillus subtilis, growth of root systems can be promoted, starch is used as leftovers, heavy metal is not contained, the cassava starch is green and pollution-free, the indole can be generated after fermentation of the cassava starch, plant growth and yield increase can be promoted, humus formed after fermentation of the fermentation substrate is mixed with microbial strains to form a microbial fertilizer, the fertilizer has microbial degradation capacity, prevention and control of nematodes can be performed, and the effect and the function are diversified.
The culture medium used in the culture process of the first microbial strain and the second microbial strain is cultured according to a standard fungus culture medium, the pH value range of the layer body is 7.2-8.3, the problem of soil acidification can be solved while nematode can be prevented, the carbon-nitrogen ratio of the microbial fertilizer on the layer body is 24-26:1, the growth of root systems and the growth of stems and leaves are inhibited when the carbon-nitrogen ratio is 24-26:1, the layer body is in a balance point when the carbon-nitrogen ratio is 25:1, the growth of the root systems and the stems and leaves is favorable at the moment, the propagation of microorganisms is promoted, the growth of the stems and leaves is favorable when the carbon-nitrogen ratio is low, the growth of the root systems is inhibited, the carbon-nitrogen ratio is controlled within the range of 24-26:1, the growth of the root systems and the stems and leaves can be effectively improved, the propagation of the microorganisms is improved, and the.
The microbial strains are attached to the surface of the base layer in a soaking mode, so that the microbial strains can be uniformly and firmly attached to the surface of the base layer, the microbial strains are prevented from being seriously lost, and meanwhile, the base layer is made of degradable materials, so that the microbial strains can be prevented and treated from nematode, and the soil pollution can not be caused.
The microbial strains slowly permeate into the soil, the degradation effect of the microbial strains and the prevention and control effect of nematodes can be improved, and meanwhile, the microbial strains are soaked in the base layer and can be adhered to the base layer in high concentration, so that the activity of the microbial strains is improved.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims (9)

1. A microbial fertilizer for controlling nematodes, comprising: the fermentation substrate, a first microbial strain, a second microbial strain, a first base layer and a second base layer; it is characterized in that the preparation method is characterized in that,
the fermentation substrate is fermented in a fermentation tank to form humus; performing activation culture on the first microbial strain and the second microbial strain to form a first culture solution and a second culture solution, and soaking the first substrate and the second substrate in the first culture solution and the second culture solution respectively;
at least one side of the first base layer and the second base layer is coated with a fermentation substrate, and the first base layer and the second base layer are compressed to form a layered body; the surface body covers the soil surface or is buried in a shallow layer of the soil, the fermentation substrate on the surface body is mixed with microbial strains for slow-release degradation, and the surface body can be used for nematode prevention and control.
2. The microbial fertilizer for controlling nematodes according to claim 1, wherein: the first microorganism strain comprises at least one of bacillus subtilis, paecilomyces lilacinus, pythium oligandrum, trichoderma viride, bacillus pumilus, bacillus amyloliquefaciens and pseudomonad defensive bacteria.
3. The microbial fertilizer for controlling nematodes according to claim 1, wherein: the second microorganism strain comprises at least one of bacillus subtilis, paecilomyces lilacinus, pythium oligandrum, trichoderma viride, bacillus pumilus, bacillus amyloliquefaciens and pseudomonad defensive bacteria.
4. The microbial fertilizer for controlling nematodes according to claim 1, wherein: anaerobic fermentation biological bacteria are added into the fermentation tank.
5. The microbial fertilizer for controlling nematodes according to claim 1, wherein: the fermentation substrate comprises one of cassava starch, pepper cake or pepper seed powder.
6. The microbial fertilizer for controlling nematodes according to claim 1, wherein: the first base layer and the second base layer are made of degradable materials.
7. The microbial fertilizer for controlling nematodes according to claim 2 or 3, wherein: and the culture medium used in the culture process of the first microbial strain and the second microbial strain is cultured according to standard fungus culture medium ingredients.
8. The microbial fertilizer for controlling nematodes according to any one of claims 1 to 6, wherein: the pH value interval of the laminar body is 7.2-8.3.
9. The microbial fertilizer for controlling nematodes according to claim 1, wherein: the carbon-nitrogen ratio of the microbial fertilizer on the laminated body is 24-26: 1.
CN201911009446.XA 2019-10-23 2019-10-23 Microbial fertilizer for preventing and treating nematodes Pending CN110698276A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170215431A1 (en) * 2011-06-23 2017-08-03 Agrinos AS AGRICULTURAL USES OF HYTd
CN108101679A (en) * 2018-01-10 2018-06-01 成都金昌旺农业科技有限公司 A kind of preparation method for inhibiting soil nematodes fertilizer
CN108546198A (en) * 2018-07-11 2018-09-18 丽水地乐农业科技合伙企业(普通合伙) Slow release fertilizer and its preparation method and application
CN108976065A (en) * 2018-07-26 2018-12-11 山东土秀才生物科技有限公司 A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode
WO2019033124A1 (en) * 2017-08-11 2019-02-14 California Safe Soil, LLC Agricultural admixtures
CN110117562A (en) * 2019-05-07 2019-08-13 黑龙江禾野科技有限公司 A kind of microbial bacterial agent of efficiently decomposed crop material

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170215431A1 (en) * 2011-06-23 2017-08-03 Agrinos AS AGRICULTURAL USES OF HYTd
WO2019033124A1 (en) * 2017-08-11 2019-02-14 California Safe Soil, LLC Agricultural admixtures
CN108101679A (en) * 2018-01-10 2018-06-01 成都金昌旺农业科技有限公司 A kind of preparation method for inhibiting soil nematodes fertilizer
CN108546198A (en) * 2018-07-11 2018-09-18 丽水地乐农业科技合伙企业(普通合伙) Slow release fertilizer and its preparation method and application
CN108976065A (en) * 2018-07-26 2018-12-11 山东土秀才生物科技有限公司 A kind of biological compound fertilizer and its processing method for preventing and treating root-knot nematode
CN110117562A (en) * 2019-05-07 2019-08-13 黑龙江禾野科技有限公司 A kind of microbial bacterial agent of efficiently decomposed crop material

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