CN110117562A - A kind of microbial bacterial agent of efficiently decomposed crop material - Google Patents
A kind of microbial bacterial agent of efficiently decomposed crop material Download PDFInfo
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- CN110117562A CN110117562A CN201910373387.8A CN201910373387A CN110117562A CN 110117562 A CN110117562 A CN 110117562A CN 201910373387 A CN201910373387 A CN 201910373387A CN 110117562 A CN110117562 A CN 110117562A
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- bacterium
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- microbial bacterial
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of microbial bacterial agents of efficiently decomposed crop material, are related to bacteria agent made of microbial bacterial agent field more particularly to a kind of multi-strain bacteria mixed fermentation.Decomposed high-efficient, use cost is low, and has a large amount of effective microbe in digest process, and balanced soil pH value improves soil, is passivated heavy metal-polluted soil, improves ecological environment of soil, is conducive to soil water-reataining fertilizer-keeping, improves the ability that crop root absorbs nutrient.To further increase stalk resource effective use, ecological environmental protection reduces pollution etc. and plays impetus.
Description
Technical field
The present invention provides a kind of microbial bacterial agents of efficiently decomposed crop material, are related to microbial bacterial agent field, especially
What is involved is bacteria agents made of a kind of multi-strain bacteria mixed fermentation.
Background technique
With the development of modern society, the development of China's agricultural has welcome new opportunities and challenges, and chemical fertilizer, pesticide are at me
The application popularization of state also makes agricultural that historic leap have occurred, and agricultural enters the chemical epoch from natural form, chemical farming
Development is great to pushing agricultural development and raising crop yield to play the role of.But agricultural crop straw deep processing, comprehensive benefit
With reducing pollution by pesticides, also always the center topic of China's agricultural, stalk remnants especially in cornstalk, contain a large amount of
There are a large amount of larva ovum and carrier, be difficult to remove in crushing process, can grow up quickly after being embedded in soil, easily initiation disease pest
Evil causes the agriculture underproduction, causes damages.Based on this, the microbial bacterial agent of decomposed stalk be solve subproblem good method it
One.
Summary of the invention
The main object of the present invention is to provide a kind of microbial bacterial agent that can significantly improve straw decomposing speed.Decomposed efficiency
Height, use cost is low, and has a large amount of effective microbe in digest process, balanced soil pH value, improves soil, passivation soil
Earth heavy metal improves ecological environment of soil, is conducive to soil water-reataining fertilizer-keeping, improves the ability that crop root absorbs nutrient.To realize
Foregoing invention purpose, the technical scheme adopted by the invention is that: a kind of decomposed fermentative microorganism microbial inoculum of efficient straw, by bacterium,
Actinomyces, fungi, bacillus subtilis, pseudomonas aeruginosa, aspergillus niger, simple mould composition.
The present invention is realized by the following method:
(1) preparation of microbial bacterial agent:
By isolated thermophilic actinomycete Thermoactinomyces sp GF1 and Thermoactinomyces sp GF2
It is mixed to prepare microbial inoculum in 2: 5: 3 ratio, basidiomycetes Coprinus sp MF1 and Coprinus spMF2 is pressed into 3:2
Ratio be mixed to prepare lignin degradation microbial inoculum, by cellulose-degrading bacteria Trichoderma sp XF1 and Rhizopus sp
XF4 is mixed to prepare cellulose-degrading bacteria in the ratio of 3:2；
(2) it ferments:
Two plants of bacterium are inoculated into the plate of the culture medium containing PDA respectively, is placed in 55 DEG C of incubator and cultivates 5-7 days, to
Spore is eluted respectively, is collected using sterile water after growing generation spore on plate by two plants of bacterium, obtains the spore of two plants of bacterium respectively
Sub- suspension.It is centrifuged using spore suspension of the supercentrifuge to two plants of bacterium, centrifugal speed is 8000 r/min, obtains two plants of bacterium
Spore.Then the spore dilution of two plants of bacterium is prepared into the suspension that concentration is every milliliter of 1 × 109 spore respectively.
Strain is collected in 36 counties and cities in Heilongjiang Province, chooses after nearly thousand bacterial strain well-chosens.Pass through different function
Microbial strains be combined proportion, through special zymotechnique, have and promote various organic matters and nitrogen, phosphorus, potassium in stalk
Equal elements decompose returning to the field, the special bacteria agent of the multi-functionals such as prevention and treatment plant root disease.
Since Heilongjiang Province is located in China northernmost, winter is very long, and very cold is dry, and fetch water, institute big in task difficulty
Be typically chosen in front of early spring spring ploughing with the straw compost time or after autumn harvest before winter approached it is more appropriate.Spring stalk stack retting is answered
In annual mid or late March between mid or late April.
Specific example one, experiment place Heilongjiang Province is the town Harbin City Wang Gang, using maize straw manure mode.
(1) stalk pre-processing:
Firstly, with stalk Jian1.5 meter Gao base, 4 meters of width or more.
Secondly, the uniform sprinkling on base's stalk, watering amount about accounts for the 60% of base's stalk weight.
(2) fungus scattering agent and urea.
Stalk: microbial inoculum: urea ratio 1:2:2.That is 1 ton of stalk, 2 kilograms of microbial inoculums, 2 kilograms of urea.Microbial inoculum, urea are pressed
After fixed proportion mixing, then identical weight soil is admixed, uniformly shed on base's stalk, appropriate soil, which is added, facilitates microbial inoculum
Uniformly shed.
(3) heap is built.
Upper layer stalk is covered with machinery, the high entirety of stalk heap reaches 3 meters or more.When covering upper layer stalk, side stalks mulching
Side watering, appropriate soil is mixed in stalk heap, facilitates stalk heap moisturizing.Secondary watering amount reaches the 60% of upper layer stalk weight
More than.
It builds after the completion of heap, it is startup stage respectively that central temperature variation, which is divided into four-stage, in stalk heap, the temperature rise period,
Hot stage, temperature-fall period, temperature reaches 50-70 degree in 7-10 days stalk heaps, and after 60 days, stalk heap central temperature drops to 50 degree
Hereinafter, straw decomposing finishes.
Experimental result is shown, 2/3rds is relatively reduced before stalk heap volume is more decomposed, the total loss of weight of stalk is greater than 50%.
Respectively by not decomposed stalk and it is decomposed after stalk, it is 3-5 minutes static with clear water by the mixing of 1:5 ratio,
Solution colour notable difference, control group color do not change substantially, and experimental group color is in buff yellow；
Laboratory testing experimental group deep yellow solution ingredient, as the result is shown high concentration humic acid.Nutriment in stalk after decomposed
Liter in content, wherein 11 .0-13.0g/kg of full nitrogen, full phosphorus 0.45-0 .66g/kg, full potassium 25.0-27 .6g/kg.
Fig. 1 Fertilizer Transformed data
Experimental data shows that the soil of continuous administration 3 years or more decomposition stalks, soil fertility obviously increases, while can reduce spring ploughing
The 21-31% of base fertilizer dosage.When outdoor environment is at 10 DEG C or more, composite bacteria agent fermentation capable of fast starting, in most suitable environment
Under, 8-10 days compost temperatures can be rapidly increased to 60 DEG C or more, 5 weeks or so can complete decomposed various crop materials.Meanwhile it can be with
Kill disease, worm's ovum and the weeds in stalk.
In conclusion microbial inoculum of the present invention accelerates straw decomposing speed, efficiently quickly decomposition corn, rice, wheat etc. are made
Object stalk, and it is at low cost, effect is good.The organic matter in soil is increased after straw decomposition, so as to improve soil physical property, is mentioned
High soil fertility.The straw decomposing inoculant is directly acted on stalk, by straw directly returning to field, crop straw burning had both been solved and has brought
Problem of environmental pollution, and stalk resource can be efficiently used, to further increase stalk resource effective use, ecological environment is protected
Shield reduces pollution etc. and plays impetus.
The foregoing is only a preferred embodiment of the present invention, the scope of protection of the present invention is not limited to this, it is any ripe
Know those skilled in the art within the technical scope of the present disclosure, the letter for the technical solution that can be become apparent to
Altered or equivalence replacement are fallen within the protection scope of the present invention.
Claims (3)
1. a kind of microbial bacterial agent of efficiently decomposed crop material, it is characterized in that: multi-strain bacteria mixed fermentation is made.
2. a kind of microbial bacterial agent of efficiently decomposed crop material according to claim 1, which is characterized in that the fermentation
The raw material of microbial inoculum are as follows: by bacterium, actinomyces, fungi, bacillus subtilis, pseudomonas aeruginosa, aspergillus niger, simple mould.
3. one according to claim 1 it is a kind of efficiently decomposed crop material microbial bacterial agent, the preparation method is as follows:
(1) preparation of microbial bacterial agent:
By isolated thermophilic actinomycete Thermoactinomyces sp GF1 and Thermoactinomyces sp GF2
It is mixed to prepare microbial inoculum in 2: 5: 3 ratio, basidiomycetes Coprinus sp MF1 and Coprinus spMF2 is pressed into 3:2
Ratio be mixed to prepare lignin degradation microbial inoculum, by cellulose-degrading bacteria Trichoderma sp XF1 and Rhizopus sp
XF4 is mixed to prepare cellulose-degrading bacteria in the ratio of 3:2；
(2) it ferments:
Two plants of bacterium are inoculated into the plate of the culture medium containing PDA respectively, is placed in 55 DEG C of incubator and cultivates 5-7 days, to
Spore is eluted respectively, is collected using sterile water after growing generation spore on plate by two plants of bacterium, obtains the spore of two plants of bacterium respectively
Sub- suspension is centrifuged using spore suspension of the supercentrifuge to two plants of bacterium, and centrifugal speed is 8000 r/min, obtains two plants of bacterium
Then the spore dilution of two plants of bacterium is prepared into the suspension that concentration is every milliliter of 1 × 109 spore respectively by spore.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910373387.8A CN110117562A (en) | 2019-05-07 | 2019-05-07 | A kind of microbial bacterial agent of efficiently decomposed crop material |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910373387.8A CN110117562A (en) | 2019-05-07 | 2019-05-07 | A kind of microbial bacterial agent of efficiently decomposed crop material |
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| CN201910373387.8A Pending CN110117562A (en) | 2019-05-07 | 2019-05-07 | A kind of microbial bacterial agent of efficiently decomposed crop material |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698276A (en) * | 2019-10-23 | 2020-01-17 | 中国农业科学院烟草研究所 | Microbial fertilizer for preventing and treating nematodes |
| CN112457857A (en) * | 2020-10-31 | 2021-03-09 | 兰州资源环境职业技术学院 | Soil conditioner for removing heavy metals in coal mining area |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424614A (en) * | 2011-09-16 | 2012-04-25 | 昆明理工大学 | Three-stage inoculation process and microbial agents for composting lignocelluloses raw material |
-
2019
- 2019-05-07 CN CN201910373387.8A patent/CN110117562A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102424614A (en) * | 2011-09-16 | 2012-04-25 | 昆明理工大学 | Three-stage inoculation process and microbial agents for composting lignocelluloses raw material |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110698276A (en) * | 2019-10-23 | 2020-01-17 | 中国农业科学院烟草研究所 | Microbial fertilizer for preventing and treating nematodes |
| CN112457857A (en) * | 2020-10-31 | 2021-03-09 | 兰州资源环境职业技术学院 | Soil conditioner for removing heavy metals in coal mining area |
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Application publication date: 20190813 |
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