CN107445694A - A kind of composite bio-chemical preparation and its applied in straw-returning - Google Patents

A kind of composite bio-chemical preparation and its applied in straw-returning Download PDF

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Publication number
CN107445694A
CN107445694A CN201710682227.2A CN201710682227A CN107445694A CN 107445694 A CN107445694 A CN 107445694A CN 201710682227 A CN201710682227 A CN 201710682227A CN 107445694 A CN107445694 A CN 107445694A
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chemical
composite
straw
stalk
composite bio
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张雅琪
张军
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张雅琪
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The present invention provides a kind of composite bio-chemical preparation, and the composite bio-chemical preparation includes straw decomposing preparation and compost fermentation preparation, and the compost fermentation preparation is:Urea:Lime:Feces of livestock and poultry is according to 45:5‑7:10 15 weight is than preparing, and the straw decomposing preparation is by composite bacteria liquid and carrier 1:4 weight is than preparing.Utilize composite bio-chemical preparation of the present invention, material obtains thoroughly decomposed during stalk processing, produce a large amount of functional microorganisms and a variety of metabolites such as plant hormone, antibiotic etc., so as to stimulate crop growth, soil nutrient is improved, improved soil structure, improves chemical fertilizer utilization ratio, its is easy to operate, beneficial to production.

Description

A kind of composite bio-chemical preparation and its applied in straw-returning
Technical field
The invention belongs to stalk resource comprehensive application technology field, and in particular to a kind of composite bio-chemical preparation and its in straw The application of stalk also Tanaka.
Background technology
Modern agriculture cultivated area is big, and agricultural production causes stalk excessive, and the open incineration of stalk causes resource unrestrained The atmosphere pollution for taking and verifying, but because that can not be fully utilized, burn phenomenon and remain incessant after repeated prohibition, composition whole society focus of attention is asked Topic.Straw-returning is the stalk that should not directly make feed(Wheat straw, corn stalk and rice straw etc.)After direct or accumulation is decomposed A kind of method in being manured into soil.The process of agricultural production is also the process of an energy conversion.Crop will in growth course Continuous consumed energy, it is also desirable to energy, constantly regulate soil reclaimed water, fertilizer, the content of air and heat is continuously replenished.Containing big in stalk Amount fresh organic materials, giving back after farmland, through decay after a while, it is possible to change into organic matter and Available nutrient.Both improve soil physical and chemical property, also be available for answering the nutrients such as certain potassium.Straw-returning can promote agricultural water conservation, section Cost, volume increase, synergy, also should fully it be paid attention in environmental protection and agricultural sustainable development.Straw-returning is in the world today The well stimulation of one culture fertility of most attention, also have while the atmosphere pollution caused by having prevented crop straw burning and increase Fertile production-increasing function.Straw-returning can increase the soil organism, and improved soil structure makes loosing soil, porosity increase, and capacity subtracts Gently, the development of microbial activity and crop root is promoted.Straw-returning getting fat production-increasing function is notable, can typically increase production 5%~10%, But if method is improper, the increase of soil germ is also resulted in, crop disease is aggravated and is short of seedling(Stiff seedling)Etc. bad phenomenon.Therefore take Rational straw-returning measure, can just play good field effect.
The cell inclusion balanced in stalk containing precious, including organic matter, C, N, P, K and various trace elements, It more provides the physical condition such as water retention, drainage and voidage, and these nutrition and environmental condition are more formed beneficial to soil " the organic hotbed " that earth microbial proliferation is grown, thus improve the biological of soil.If it is allowed to rotten organic growth in the ground If adding Reasonable Regulation And Control, very strong negative effect can be produced, the amount reproduction of microorganism will cause that crop " is robbed nitrogen to show As " and Xian Xian Yellowization delay long phenomenon, more seriously in the case of non-aerobic bacteria advantage, crop young root will be vulcanized stalk The infringement Er Wei Yellow of the harmful substances such as hydrogen, ammonia or methyl mercaptan.
Straw-returning has diversified forms, can be divided into 5 major classes:Stalk crushes and turns over returning to the field, straw mulching and returning, stack retting also Field, burn and recycle in field, cross abdomen returning to the field.Stalk is crushed and turns over returning to the field, is exactly crop harvesting, and the stalk after obtaining is crushed by mechanization, Arable land, is directly turned in soil.So can the advantages of have four:1st, soil physico-chemical property is improved.2nd, the nutriment of stalk Sufficiently it is retained in soil.3rd, chemical fertilizer utilization ratio is improved.4th, crop drought resistance saline-alkali tolerance is improved.
Using the extensive adaptability and multifunctionality of microorganism domestic and international scientific researcher has been increasingly subject to convert stalk Pay attention to, because microorganism has the advantages that purposes is more, nutritive value is high, the cycle is short, renewable in stover.As utilizing straw The new way of stalk resource, have it is wide should have prospect, will be played an increasingly important role in agricultural production.At present, grind System develops preferable crop material quick composting agent has turned into a focus of microorganism formulation.Such product in China agriculturally Application be in the starting stage, although some kinds have applied several years, also effective good product, most products are still deposited Improved place is wanted in many, application effect also also needs to the checking of multispots trial, technical indicator particularly in product quality, In terms of strain combination and raising agricultural product quality.Prior art it is known that energy decomposition of cellulose bacterial spore Bacillus (Bacillus), class bacillus(Paenibacillus), pseudomonas (Pseudomones), vibrio (Vibrio), Micrococcus (Micrococcus), streptococcus (Streptococcus), fusobacterium (Clostridium), Promyxobacterium (Promyxobecterium), Cytophaga (Cytophaga), raw born of the same parents' Cytophage (Sporocytophage), heap capsule Pseudomonas (Sorangium), Spirochaeta (Spirochaeta)Etc..
The key of microbial straw biological conversion has three:Microbial degradation lignin and cellulose;It is single to produce microorganism Cell protein, improve the nutritive value of stalk;Optimized production process.And how according to the strain characteristic being separated to, it is individually or multiple Close, then detect and test its capacity of decomposition, selected matched combined is even more the most important thing.
The content of the invention
The present invention, because stalk C/N is high, is difficult in its natural state in order to solve prior art during straw degradative It is decomposed by the microorganisms, so as to the cycle length for the conversion that causes after straw-returning to be decomposed in soil, it is difficult to as this season crop fertilizer The problem of source.A kind of composite bio-chemical preparation and its application in straw-returning are provided, use the composite bio-chemical preparation of the present invention Fermented stalk, obtain organic fertilizer, decomposition into field.
The composite bio-chemical preparation of the present invention, reasonable compatibility between each strain, symbiosis are coordinated, mutually not antagonism, its preparation method Simplicity, it is easy to implement the method, due to during processing material obtain thoroughly it is decomposed, produce a large amount of functional microorganisms and a variety of generations Product such as plant hormone, antibiotic etc. are thanked, so as to stimulate crop growth, soil nutrient is improved, improved soil structure, improves Chemical fertilizer utilization ratio, its is easy to operate, beneficial to production.
The present invention adopts the following technical scheme that realization:
A kind of composite bio-chemical preparation, the composite bio-chemical preparation include straw decomposing preparation and compost fermentation preparation.
The compost fermentation preparation is:Urea:Lime:Feces of livestock and poultry is according to 4-5:5-7:10-15 weight is than preparing.
The straw decomposing preparation is by composite bacteria liquid and carrier 1:4 weight is than preparing;
The composite bacteria liquid is by bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, Phanerochaete chrysosporium, denitrogenation pair ball Bacterium, Bacillus foecalis alkaligenes are according to volume ratio 3:2:4:4:5:1 volume ratio mixing.
The bacillus amyloliquefaciens are(Bacillus amyloliquefaciens)ATCC 23843;
The Li Shi trichodermas are Li Shi trichodermas(Trichodermareesei)ATCC 24449;
The trichoderma pseudokiningii is trichoderma pseudokiningii(Trichoderma pseudokiningii)CGMCC NO.3221 (referring to CN101781565)
The Phanerochaete chrysosporium is(Phanerochaete chrysosporium)ATCC 34541;
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC 13543;
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes(Alcaligenes faecalis)ATCC 31555;
The preparation method of the composite bacteria agent is as follows:By bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, yellow archespore hair It is 5-8 × 10 that flat lead fungi, Paracoccus denitrificans, Bacillus foecalis alkaligenes are cultivated to concentration respectively9Individual/ml bacterium solution, then according to 3:2: 4:4:5:1 volume ratio mixing, is produced;Composite bacteria agent in the range of this bacteria concentration, can quick composting stalk, if bacterium number compared with Small, then the time that stalk is recovered can extend, the sowing of one batch of crop so under the influence of meeting, and excessive can increase of bacterium number is trained This.
The carrier is:Chitosan, turfy soil are according to weight than 2:5 weight are than preparing;
It is used for the method for straw-returning using above-mentioned composite bio-chemical preparation, step is as follows:
(1)Stalk is collected after harvest corn, takes advantage of the blue or green section shape for being ground into 10-20cm;
(2)Milpa after harvesting digs cellar for storing things, and long 3m, wide 2m, the deep 1.5-2m at the cellar for storing things, that digs out is native standby.
(3)By step(1)The stalk of processing is piled up in cellar for storing things, and straw decomposing preparation carries the previous day and adds the dilution of 10 times of weight waters (Be advantageous to recover and strengthen the vigor and activity of strain, improve the efficiency of decomposing agent corruption stalk)Dilution microbial inoculum is obtained, according to The 25-35% addition dilution microbial inoculums of stalk weight, after being well mixed, ferment under normal temperature, fermentate temperature are detected in fermentation process, When temperature rises to more than 60 DEG C, by mixed material turning, hereafter every other day turning once, and detects the moisture content of material, Terminate to ferment when moisture content≤25%, obtain decomposed product;
(4)According to decomposed product:Compost fermentation preparation is 3:2 ratio addition compost fermentation preparation, is covered standby after well mixed With soil, ferment 10 d, obtains the organic fertilizer of stalk fermentation, is used directly for returning to the field.
The maize straw organic fertilizer digs out returning to the field from cellar for storing things, according to the amount of application of 300kg/ mus by maize straw organic fertilizer Uniformly spread fertilizer over the fields and used in field as base manure, field is turned over after spreading fertilizer over the fields.
Stubble is corn before the crop, and lower stubble is wheat;
The feces of livestock and poultry is selected from chicken manure, duck excrement, pig manure, cow dung etc..
Strain of the present invention can be from China Committee for Culture Collection of Microorganisms's common micro-organisms center And American Type Culture collection warehousing (CGMCC)(ATCC)It is commercially available.
Strain of the present invention can obtain the bacterium solution of required concentration by the cultural method of routine, as space is limited, and Do not repeat one by one.
The present invention takes advantage of blue or green crushing when being crushed to stalk, it is possible to reduce sugar loss in stalk, it is rotten to accelerating stalk Solution, increase soil nutrient play an important role;A variety of nutrients, also containing various trace elements, the energy after everfermentation are rich in stalk It is enough that enough nutrients are provided to Soil Microorganism, moreover it is possible to accumulate for the nutrient needed for plant growth;
Biological agent provided by the invention can under cryogenic 10-18 DEG C quickening the quick returning to the field of stalk, the microbial inoculum microorganism Between there is good cooperative effect;There is efficient degradation property to corn, the stalk such as rice for being difficult to degrade, especially low Under the conditions of temperature;It is strong to adapt to external environment ability;The microbial inoculum is mainly used in crop material decomposition into field in agricultural planting, by its turn Turn to efficient, safe and environment-friendly biological organic fertilizer.Can be decomposed rapidly by field straw at one week or so, relative to prior art Microbial inoculum greatly reduce the decomposed time, solve the problems, such as to exist in crops straw returning field decomposed slow, returning to the field difficulty, improve Soil organic matter content, serve the effect of " land used is increased soil fertility ", cracked for a long time in straw-returning problem government encourage, The quagmire that peasant slacks off.
Microbe species collocation of the present invention is reasonable, with strong complementarity by the synergy of several different strains, It can rapidly be bred using rear composite bacteria agent, form dominant population, and can be brought rapidly up, promote the decomposition of stalk, and heap The nutrient content such as organic matter is high after corruption;Can utilize dominant bacteria destroy stalk eucaryotic cell structure, effectively facilitate starch, protein, The dissolution of the active ingredients such as cellulose, lignin, so that the protein of most of difficult degradation, cellulose, lignin etc. aoxidize Decompose, to mitigate the pressure of subsequent degradation, and, produce more than 60 DEG C high temperature come accelerate maize straw degrade speed, overcome The problem of Straw decomposing transformation period caused by northern temperature is relatively low is long;
The application adds feces of livestock and poultry during the fermentation can accelerate to form humus as straw degradative accelerator, urea, promote Enter the formation of crop fertilizer, be advantageous to the shortening of degradation cycle, and nitrogen, phosphorus, potassium element in the Straw manures prepared can be made Content is high, is advantageous to further increase soil fertility.
Embodiment
Embodiment 1:
A kind of composite bio-chemical preparation, the composite bio-chemical preparation include straw decomposing preparation and compost fermentation preparation.
The compost fermentation preparation is:Urea:Lime:Feces of livestock and poultry is according to 4:5:10 weight is than preparing.
The straw decomposing preparation is by composite bacteria liquid and carrier 1:4 weight is than preparing;
The composite bacteria liquid is by bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, Phanerochaete chrysosporium, denitrogenation pair ball Bacterium, Bacillus foecalis alkaligenes are according to volume ratio 3:2:4:4:5:1 volume ratio mixing.
The bacillus amyloliquefaciens are(Bacillus amyloliquefaciens)ATCC 23843;
The Li Shi trichodermas are Li Shi trichodermas(Trichodermareesei)ATCC 24449;
The trichoderma pseudokiningii is trichoderma pseudokiningii(Trichoderma pseudokiningii)CGMCC NO.3221 (referring to CN101781565)
The Phanerochaete chrysosporium is(Phanerochaete chrysosporium)ATCC 34541;
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC 13543;
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes(Alcaligenes faecalis)ATCC 31555;
The preparation method of the composite bacteria agent is as follows:By bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, yellow archespore hair It is 5 × 10 that flat lead fungi, Paracoccus denitrificans, Bacillus foecalis alkaligenes are cultivated to concentration respectively9Individual/ml bacterium solution, then according to 3:2:4: 4:5:1 volume ratio mixing, is produced;Composite bacteria agent in the range of this bacteria concentration, can quick composting stalk, if bacterium number compared with Small, then the time that stalk is recovered can extend, the sowing of one batch of crop so under the influence of meeting, and excessive can increase of bacterium number is trained This.
The carrier is:Chitosan, turfy soil are according to weight than 2:5 weight are than preparing;
It is used for the method for straw-returning using above-mentioned composite bio-chemical preparation, step is as follows:
(1)Stalk is collected after harvest corn, takes advantage of the blue or green section shape for being ground into 10-20cm;
(2)Milpa after harvesting digs cellar for storing things, and long 3m, wide 2m, the deep 1.5-2m at the cellar for storing things, that digs out is native standby.
(3)By step(1)The stalk of processing is piled up in cellar for storing things, and straw decomposing preparation carries the previous day and adds the dilution of 10 times of weight waters (Be advantageous to recover and strengthen the vigor and activity of strain, improve the efficiency of decomposing agent corruption stalk)Dilution microbial inoculum is obtained, according to The 25-35% addition dilution microbial inoculums of stalk weight, after being well mixed, ferment under normal temperature, fermentate temperature are detected in fermentation process, When temperature rises to more than 60 DEG C, by mixed material turning, hereafter every other day turning once, and detects the moisture content of material, Terminate to ferment when moisture content≤25%, obtain decomposed product;
(4)According to decomposed product:Compost fermentation preparation is 3:2 ratio addition compost fermentation preparation, is covered standby after well mixed With soil, ferment 10 d, obtains the organic fertilizer of stalk fermentation, is used directly for returning to the field.
The maize straw organic fertilizer digs out returning to the field from cellar for storing things, according to the amount of application of 300kg/ mus by maize straw organic fertilizer Uniformly spread fertilizer over the fields and used in field as base manure, field is turned over after spreading fertilizer over the fields.
Stubble is corn before the crop, and lower stubble is wheat;
The feces of livestock and poultry is selected from chicken manure.
Strain of the present invention can be from China Committee for Culture Collection of Microorganisms's common micro-organisms center And American Type Culture collection warehousing (CGMCC)(ATCC)It is commercially available.
Embodiment 2:
A kind of composite bio-chemical preparation, the composite bio-chemical preparation include straw decomposing preparation and compost fermentation preparation.
The compost fermentation preparation is:Urea:Lime:Feces of livestock and poultry is according to 5:7:15 weight is than preparing.
The straw decomposing preparation is by composite bacteria liquid and carrier 1:4 weight is than preparing;
The composite bacteria liquid is by bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, Phanerochaete chrysosporium, denitrogenation pair ball Bacterium, Bacillus foecalis alkaligenes are according to volume ratio 3:2:4:4:5:1 volume ratio mixing.
The bacillus amyloliquefaciens are(Bacillus amyloliquefaciens)ATCC 23843;
The Li Shi trichodermas are Li Shi trichodermas(Trichodermareesei)ATCC 24449;
The trichoderma pseudokiningii is trichoderma pseudokiningii(Trichoderma pseudokiningii)CGMCC NO.3221 (referring to CN101781565)
The Phanerochaete chrysosporium is(Phanerochaete chrysosporium)ATCC 34541;
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC 13543;
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes(Alcaligenes faecalis)ATCC 31555;
The preparation method of the composite bacteria agent is as follows:By bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, yellow archespore hair It is 8 × 10 that flat lead fungi, Paracoccus denitrificans, Bacillus foecalis alkaligenes are cultivated to concentration respectively9Individual/ml bacterium solution, then according to 3:2:4: 4:5:1 volume ratio mixing, is produced;Composite bacteria agent in the range of this bacteria concentration, can quick composting stalk, if bacterium number compared with Small, then the time that stalk is recovered can extend, the sowing of one batch of crop so under the influence of meeting, and excessive can increase of bacterium number is trained This.
The carrier is:Chitosan, turfy soil are according to weight than 2:5 weight are than preparing;
It is used for the method for straw-returning using above-mentioned composite bio-chemical preparation, step is as follows:
(1)Stalk is collected after harvest corn, takes advantage of the blue or green section shape for being ground into 10-20cm;
(2)Milpa after harvesting digs cellar for storing things, and long 3m, wide 2m, the deep 1.5-2m at the cellar for storing things, that digs out is native standby.
(3)By step(1)The stalk of processing is piled up in cellar for storing things, and composite bacteria agent carries the previous day and adds the dilution of 10 times of weight waters(Have Beneficial to the vigor and activity for recovering and strengthening strain, the efficiency of raising decomposing agent corruption stalk)Dilution microbial inoculum is obtained, according to stalk The 25-35% addition dilution microbial inoculums of weight, after being well mixed, ferment under normal temperature, fermentate temperature are detected in fermentation process, works as temperature For degree when rising to more than 60 DEG C, by mixed material turning, hereafter every other day turning once, and detects the moisture content of material, when containing Terminate to ferment during water rate≤25%, obtain decomposed product;
(4)According to decomposed product:Compost fermentation preparation is 3:2 ratio addition compost fermentation preparation, is covered standby after well mixed With soil, ferment 10 d, obtains the organic fertilizer of stalk fermentation, is used directly for returning to the field.
The maize straw organic fertilizer digs out returning to the field from cellar for storing things, according to the amount of application of 300kg/ mus by maize straw organic fertilizer Uniformly spread fertilizer over the fields and used in field as base manure, field is turned over after spreading fertilizer over the fields.
Stubble is corn before the crop, and lower stubble is wheat;
The feces of livestock and poultry is selected from cow dung.
Strain of the present invention can be from China Committee for Culture Collection of Microorganisms's common micro-organisms center And American Type Culture collection warehousing (CGMCC)(ATCC)It is commercially available.
Influence of the crops straw returning field of embodiment 3 to topsoil soils
Increase soil fertility using embodiment 1-2 maize straw method, wherein, corn stalk during maize straw machinery returning to the field The amount of application of stalk is 300kg/ mus, respectively collection experiment before and experiment 1 year after topsoil soils, analyze its basic physics and chemistry number Value, concrete outcome are shown in Table 1:
Influence of the straw-returning mode of table 1 to soil
Index parameter Organic matter(g/kg) Full nitrogen(g/kg) Phosphorus(g/kg) Potassium(g/kg) Unit weight(g/cm3 Total porosity(%)
Soil base value 16.74 1.33 17.8 213.42 1.44 40.31
Maize straw machinery returning to the field 18.28 1.42 18.5 232.43 1.36 41.07
Embodiment 1 26.34 2.62 29.74 295.63 1.12 43.75
Embodiment 2 27.21 2.73 29.98 293.25 1.13 43.92
As it can be seen from table 1 after using embodiment 1-2 maize straw method, the physicochemical property of soil has obtained very big Change, not only Nutrient Elements in Soil content substantial increase, fertility greatly improves, and also reduces the soil weight, increase Soil porosity is added.And by the way of maize straw machinery returning to the field, nitrogen, P elements increment in the rotten rear soil of stalk It is smaller for embodiment 1 and embodiment 2, because the nitrogen P elements contained in maize straw are relatively low, and nitrogen P elements will also be influenceed by soil environment (air, temperature, moisture, microbial activities) and crop vigor and respiratory intensity, Content can be reduced further, in order to maintain plant growth, generally required and applied composite fertilizer in addition, and embodiment 1 and embodiment 2 exist Just microorganism normal growth, and remaining a large amount of nitrogen, phosphorus, potassium elements are maintained when fermentation supplemented with nitrogen, phosphorus, potassium element It can also be retained in the Straw manures that fermentation obtains, the needs of edaphon and plant growth are disclosure satisfy that after returning to the field.
Acted synergistically between the application biological agent composition of embodiment 4
Using composite bacteria liquid made from embodiment one as experimental group;
Compare one group:Bacillus amyloliquefaciens are not added, remaining is the same as embodiment 1;
Compare two groups:Li Shi trichodermas are not added, and remaining is the same as embodiment 1;
Compare three groups:Trichoderma pseudokiningii is not added, remaining is the same as embodiment 1;
Compare four groups:Phanerochaete chrysosporium is not added, remaining is the same as embodiment 1;
Compare five groups:Paracoccus denitrificans is not added, remaining is the same as embodiment 1;
Compare six groups:Bacillus foecalis alkaligenes is not added, remaining is the same as embodiment 1.
Blank control group:Composite bacteria liquid is not added, only adds the blank control group of water,
8 experimental groups altogether, 2 repetitions are tested.Take fresh stalk to be intercepted, ensure that the stalk of every group of experiment is suitable, keep Apply that water, temperature, humidity, sunlight sunshine condition are consistent, and other conditions are also essentially identical.
Respectively by eight experimental group composite bacteria liquids(Blank group only water)Carrying the previous day adds the dilution of 10 times of water to obtain dilution activation Liquid, activating solution is diluted according to 20% addition of stalk weight,
All experimental groups are placed in identical environment and daily Timing measurement temperature, humidity change, and the decomposed degree of stalk.
The calculation formula of resolution ratio is:
Rate of corrosion (part)=(weight of the decomposed preceding stalk of weight/carry out of stalk after decomposed) × 100%
After 7 days, the decomposition that the stalk in eight test groups is compared in terms of color, feel and stretching resistance becomes fermentation maturity at the same time Change situation is referring to table 2;
The straw decomposing contrast test of table 2
Embodiment 1 Control one Control two Control three Control four Control five Control six Blank control
Color Dark-grey rust Brown rust Brown rust Grey Grey It is dark blue or green It is dark blue or green Without significant change
Feel It is very soft It is slightly soft It is slightly soft It is soft It is slightly soft It is slightly soft It is slightly soft Firmly
Smell It is very smelly Micro- corruption It is slightly smelly It is smelly It is smelly Micro- corruption Micro- corruption Without significant change
Rate of corrosion 69% 33% 35% 41% 37% 21% 26% 10%
Influence of the 5 direct decomposition into field of embodiment to crop yield.
Stubble is the plot of corn before selection, and plot is divided into three equal parts, in the case of other cultivation condition identicals, Do following three kinds of processing:
Test 1 group:By preceding stubble corn decomposition into field, using the method for embodiment 1, by maize straw full dose returning to the field on the spot, then Wheat is planted, no longer applies other fertilizer during wheat growth;
Test 2 groups:By preceding stubble corn decomposition into field, using the method for embodiment 2, by maize straw full dose returning to the field on the spot, then Wheat is planted, no longer applies other fertilizer during wheat growth;
Control group:Then preceding stubble corn plants wheat, 15-15-15 N P and Ks are applied according to conventional amount used in wheat growth NPK;
Under three kinds of dispositions, the rice per mu yield situation in each plot is as shown in table 3.
Each plot wheat per mu yield situation of table 3
Area(Mu) Yield(Kg/ mus)
Test 1 group 4.1 604.7
Test 2 groups 3.7 617.5
Control group 2 543.5
As can be seen from the above table, by after the direct decomposition into field of maize straw, while succession crop does not apply fertilizer, wheat is still So increase production 11.3% and 13.6% respectively than Routine control group, it can be seen that, maize straw method of the invention can change Kind soil physico-chemical property, increases soil fertility, reduces the administration of chemical fertilizer, and improve crop yield.
Although above detailed explanation is made to this case with generality explanation and embodiment, in the present invention On the basis of, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not Deviate the modification or improvement made on the basis of present invention spirit, belong to the scope of protection of present invention.

Claims (8)

1. a kind of composite bio-chemical preparation, the composite bio-chemical preparation includes straw decomposing preparation and compost fermentation preparation.
2. composite bio-chemical preparation according to claim 1, it is characterised in that the compost fermentation preparation is:Urea:Stone Ash:Feces of livestock and poultry is according to 4-5:5-7:10-15 weight is than preparing.
3. the composite bio-chemical preparation according to claim 1-2, it is characterised in that the straw decomposing preparation is by composite bacteria liquid With carrier 1:4 weight is than preparing.
4. the composite bio-chemical preparation according to claim 1-3, it is characterised in that the composite bacteria liquid is by solution starch gemma bar Bacterium, Li Shi trichodermas, trichoderma pseudokiningii, Phanerochaete chrysosporium, Paracoccus denitrificans, Bacillus foecalis alkaligenes are according to volume ratio 3:2:4:4: 5:1 volume ratio mixing.
5. the composite bio-chemical preparation according to claim 1-4, it is characterised in that
The bacillus amyloliquefaciens are(Bacillus amyloliquefaciens)ATCC 23843;
The Li Shi trichodermas are Li Shi trichodermas(Trichodermareesei)ATCC 24449;
The trichoderma pseudokiningii is trichoderma pseudokiningii(Trichoderma pseudokiningii)CGMCC NO.3221;
The Phanerochaete chrysosporium is(Phanerochaete chrysosporium)ATCC 34541;
The Paracoccus denitrificans is (Paracoccus denitrificans) ATCC 13543;
The Bacillus foecalis alkaligenes is Bacillus foecalis alkaligenes(Alcaligenes faecalis)ATCC 31555.
6. the composite bio-chemical preparation according to claim 1-5, it is characterised in that the preparation method of the composite bacteria agent is such as Under:By bacillus amyloliquefaciens, Li Shi trichodermas, trichoderma pseudokiningii, Phanerochaete chrysosporium, Paracoccus denitrificans, Bacillus foecalis alkaligenes It is 5-8 × 10 to cultivate respectively to concentration9Individual/ml bacterium solution, then according to 3:2:4:4:5:1 volume ratio mixing, is produced.
7. the composite bio-chemical preparation according to claim 1-6, it is characterised in that the carrier is:Chitosan and turfy soil are pressed According to weight than 2:5 weight are than preparing.
8. the stalk fertilizer prepared using the composite bio-chemical preparation described in claim 1-7.
CN201710682227.2A 2017-08-10 2017-08-10 A kind of composite bio-chemical preparation and its applied in straw-returning Pending CN107445694A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107226760A (en) * 2017-06-29 2017-10-03 朱玲芳 A kind of method of maize straw full dose returning to the field on the spot
CN108990749A (en) * 2018-06-18 2018-12-14 林伟 A method of utilizing biological organic fertilizer field planting short-tube lycoris and culture fertility
CN111662125A (en) * 2020-07-29 2020-09-15 湖北田头生物科技有限公司 Returning-to-field corrosion-aiding biological organic fertilizer and processing method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102199564A (en) * 2011-03-28 2011-09-28 武汉合缘绿色生物工程有限公司 Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof
CN103497915A (en) * 2013-09-22 2014-01-08 东北农业大学 Complex microbial agent for composting fermentation and application thereof
CN104099282A (en) * 2014-07-19 2014-10-15 尹梦珍 Environment-friendly straw degradation agent
CN105746028A (en) * 2016-01-22 2016-07-13 杭州富阳飞博科技有限公司 Straw decomposing field-returning method capable of effectively reducing environmental pollution

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102199564A (en) * 2011-03-28 2011-09-28 武汉合缘绿色生物工程有限公司 Composite microbial agent for direct decomposition of crop straws returned to field and preparation method thereof
CN103497915A (en) * 2013-09-22 2014-01-08 东北农业大学 Complex microbial agent for composting fermentation and application thereof
CN104099282A (en) * 2014-07-19 2014-10-15 尹梦珍 Environment-friendly straw degradation agent
CN105746028A (en) * 2016-01-22 2016-07-13 杭州富阳飞博科技有限公司 Straw decomposing field-returning method capable of effectively reducing environmental pollution

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107226760A (en) * 2017-06-29 2017-10-03 朱玲芳 A kind of method of maize straw full dose returning to the field on the spot
CN108990749A (en) * 2018-06-18 2018-12-14 林伟 A method of utilizing biological organic fertilizer field planting short-tube lycoris and culture fertility
CN111662125A (en) * 2020-07-29 2020-09-15 湖北田头生物科技有限公司 Returning-to-field corrosion-aiding biological organic fertilizer and processing method thereof

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Application publication date: 20171208