CN107778035A - A kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof - Google Patents
A kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to bio-feritlizer preparing technical field, a kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof is provided, formed by fermentation substrate fermentation of agricultural wastes such as Chicken dung, powder of straw, wheat bran, furfural dregs, apply in farmland, the content of soil with organic matter, alkali-hydrolyzable nitrogen and available potassium is improved, improves crop yield and quality.The percentage by weight of each raw material is：60% decomposed chicken manure, 25% powder of straw, 5% wheat bran and 10% furfural dregs are mixed into fermentation substrate, are inoculated with by the bacterium amount that connects of fermentation substrate weight 0.5% into composite bacteria agent；The composite bacteria agent is by volume：10% bacillus amyloliquefaciens, 20% Kang Shi pseudomonads, 20% Trichoderma harzianum and the wine brewing Daqu mixing of 50% delicate fragrance type.Growth-promoting obvious effect of increasing production is applied in field.Using special composite ferment by animal wastes, the discarded object of agricultural, house refuse etc., the green fertilizer rich in beneficial microbe and organic matter is changed into, application prospect is had an optimistic view of.
Description
Technical field
The invention belongs to bio-feritlizer preparing technical field, and in particular to a kind of growth-promoting volume increase microbial organic fertilizer and its system
Preparation Method.
Background technology
China's agricultural chemicals, chemical fertilizer widely use and abused the public hazards for turning into agriculturally maximum.The waste water of agricultural production, give up
Gas, waste residue, bring the heavy damage of ecological environment.House refuse, animal wastes, agriculture discarded object, while exacerbate people
The deterioration of class living environment.Blue sky and white cloud is can not see in some cities, and in the case that food security coefficient constantly declines, people are to green
Color grain, green vegetables, green fruit, green meat, eggs and milk, the demand of green environment are further strong.
Microbial organic fertilizer is according to rhizosphere soil microecology and plant nutrient physiological principle, is sent out using modern biotechnology
The microbial manure that ferment engineering technology develops.It is using multifunctional microbial active bacteria as core, is had with fertilizer type
Machine matter is carrier, then is equipped with a small amount of organic nutrient and micro- reasonable combination, is formed using advanced technique process technology
There is pollution-free, nuisanceless, the new fertilizer suitable for Development of Green Food.This bio-feritlizer can be to crops fertilizer and can
Draw into crop rhizosphere soils and be implanted with beneficial microorganism dominant group, reach green, fertilize the soil strong bacterium, growth-promoting diseases prevention, high-quality height
The purpose of production.Patent CN201310659542 provides a kind of production method of microbial organic fertilizer, matches somebody with somebody by substrate of stalk
Microbial organic fertilizer is made in the composite bacteria fermentation for closing the compositions such as bacillus subtilis, aspergillus and trichoderma.Patent
CN201510207068 is using chicken manure and bacteria residue as primary raw material, by adding complex micro organism fungicide, function strain Rapid Fermentation
Into the organic fertilizer of high quality.
Furfural dregs are biomass class material such as corncob, cornstalk, rice husk, cotton seed hulls and agricultural byproducts processing leftover bits and pieces
In poly-pentose composition hydrolysis production furfural (furtural) caused by biomass class discarded object.Furfural dregs are as biomass by hydrolyzation
During caused discarded object, its salt content is high, can produce pollution to air, soil, river in acidity, its bulk deposition.
Furfural dregs contain substantial amounts of cellulose, hemicellulose, lignin as a kind of biomass class discarded object, have good recycling
Value.Therefore, reasonably recycling furfural dregs, eliminate its pollution to environment, while the economy for increasing furfural dregs is added
Value, realizes the zero release of pollutant target in furfural enterprise production process, the purpose for reach clean manufacturing, recycling, is chaff
The problem of aldehyde slag recycling urgent need to resolve.
The content of the invention
The invention provides a kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof, with decomposed chicken manure, powder of straw,
The agricultural wastes such as wheat bran, furfural dregs are fermentation substrate, prepare microbial organic fertilizer, are applied in farmland, are improved organic in soil
The content of matter, alkali-hydrolyzable nitrogen and available potassium, improve crop yield and quality.
What the present invention was realized by following technical scheme：A kind of growth-promoting increases production microbial organic fertilizer, the weight percent of each raw material
Than for：60% decomposed chicken manure, 25% powder of straw, 5% wheat bran and 10% furfural dregs are mixed into fermentation substrate, by fermentation substrate weight
0.5% bacterium amount that connects is inoculated with into composite bacteria agent；The composite bacteria agent is by volume：10% bacillus amyloliquefaciens（Bacillus amyloliquefaciens）, 20% Kang Shi pseudomonads（Pseudomonas costantinii）, 20% Trichoderma harzianum
（Trichoderma harzianum）With the wine brewing Daqu mixing of 50% delicate fragrance type.
The delicate fragrance type wine brewing Daqu is that the bent and rear fiery song of the red heart of delicate fragrance type white spirit by solid state method is 1 by weight：2 mixing
Mixed starters.
The specific fermentation process of Novel microbial organic fertilizer is：It is inoculated with fermentation substrate into composite bacteria agent, then by water
Point regulation is to 50%-55%, and heap pile in echelon, it is 40-45 DEG C control fermentation temperature, and fermentation 15 days, then spreading for cooling is air-dried to aqueous
Amount≤15%, bacteria containing amount >=5*109Cfu/g.
In the composite bacteria agent：Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）With Kang Shi vacation unit cells
Bacterium（Pseudomonas costantinii）Bacteria containing amount >=4*109Cfu/g, Trichoderma harzianum（Trichoderma harzianum）Bacteria containing amount >=3*108cfu/g。
The bacillus amyloliquefaciens（Bacillus amyloliquefaciens）, Kang Shi pseudomonads
（Pseudomonas costantinii）And Trichoderma harzianum（Trichoderma harzianum）It is commercially available, it is advised
Lattice meet national sector standard.Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）, Kang Shi pseudomonads
（Pseudomonas costantinii）And Trichoderma harzianum（Trichoderma harzianum）Trained according to inclined-plane culture, seed
Support, the preparation of liquid state fermentation third stage culture, specific preparation method is：Bacillus amyloliquefaciens use beef extract with Kang Shi pseudomonads
Protein culture medium culture, cultivation temperature are 25-30 DEG C, primary inclined plane culture 48 hours；By the solution starch gemma of inclined-plane culture
Bacillus or Kang Shi pseudomonads are inoculated into 150r/min in fluid nutrient medium, and seed liquor is made in 24 hours in 25-30 DEG C of culture；Will kind
Sub- liquid is inoculated in the fermentation tank of the beef extract-peptone fluid nutrient medium equipped with sterilizing for 1%-5% inoculum concentration by volume,
Tank internal pressure is 0.02-0.04MPa, 37 DEG C, 1.8 ± 0.2mg/L of dissolved oxygen, rotating speed 150r/min of temperature, bacillus amyloliquefaciens
Incubation time is 36-40 hours, and Kang Shi pseudomonads incubation time is 40-48 hours；Trichoderma harzianum is trained with PDA culture medium
Support, cultivation temperature is 28-32 DEG C, primary inclined plane culture 48 hours；The Trichoderma harzianum of inclined-plane culture is inoculated into fluid nutrient medium
Seed liquor is made in 24 hours in middle 180r/min, 28-32 DEG C of culture；Seed liquor is inoculated in for 1%-5% inoculum concentration by volume
In the fermentation tank of PDA liquid medium equipped with sterilizing, tank internal pressure is 0.02-0.04MPa, 37 DEG C of temperature, dissolved oxygen 2.0 ±
0.2mg/L, rotating speed 180r/min, incubation time are 44-48 hours.
The trapezoidal pile base heap is wide 3 meters, and top heap is wide 1 meter, and heap is high 1 meter.
In strain of the present invention：Bacillus amyloliquefaciens：Belong to bacillus, no pod membrane, peritrichous, energy
Motion.Caused subtilin, polymyxins, nystatin, gramicidins isoreactivity material during thalli growth, these work
Property material has obvious inhibitory action to pathogenic bacteria or conditioned pathogen.Abundant metabolism product can be produced：Synthesizing a variety of has
The materials such as machine acid, enzyme, physiologically active and other a variety of nutrients being easily utilized.Leaf is lost using the undesirable root of crop and applies soil
Organic fertilizer in earth, rot to decompose, discharge nutrient, for crop utilization, and form humus, improve the structure of soil
And fertility.Kang Shi pseudomonads：Belong to Gram-negative bacteria, be widely present in soil, water, plant, animal activity environment.Can
Effectively facilitate seed to sprout, improve germinating energy, promote the root elongation effect of crop side notable.Trichoderma harzianum：Belong to Fungi Imperfecti Asia
Door, Hyphomycetes, hyphomycetales, spore mushroom is glued, be the fungi of a kind of generally existing.Its secretion passes the withered cause of disease of various crops to soil
Bacterium sickle-like bacteria has strong inhibition effect.Metabolite can improve crop seed viability, promote seed to sprout ahead of time.
Delicate fragrance type wine brewing Daqu includes bent and rear three kinds of the fire song of clear stubble song, red heart, and through test of many times, we only select red heart
Bent and rear fire is bent（1：2）As leavening, delicate fragrance type makes wine Daqu by the offer of Jin Yu white wine company.
Contain abundant microbe groups in delicate fragrance type wine brewing Daqu, such as：Aspergillus, biped capsule bacterium, Candida, folliculus
Bacterium, Mucor, Pichia pastoris, enterobacteria, acetobacter, leukonid, lactobacillus and bacillus etc., themselves all it is environment
The strong kind of middle colonization ability, this composite ferment have flora species is more, structural stability is strong, fermenting speed is fast, metabolism
The features such as activity is high.It is good production alcoholic fermentation when fermentation temperature is less than 30 DEG C using cereal crops such as sorghums as substrate
Agent.We change its fermentation temperature（40-45℃）, with the addition of disease-resistant growth-promoting functional microorganism, change with chicken manure, stalk, furfural
The agricultural wastes such as slag are fermentation substrate, and the directed change course of fermentation of composite ferment, saccharomycete no longer take advantage ground
Position, the beneficial functions microorganism of addition and Aspergillus in delicate fragrance type wine brewing Daqu and the monoid such as actinomyces turn into the master in flora
Flow and largely quickly breed；Caused a small amount of alcohol is also metabolized utilization as mesostate, in stalk and furfural dregs
Cellulose and lignin are effectively degraded, and organic materials nutrient content greatly increases.
Compared with other microbial organic fertilizers：Energy is colonized in microorganism inherently environment in delicate fragrance type wine brewing Daqu
The strong flora of power, supplement beneficial microbe after fermenting speed is fast in organic compound, can ferment for finished product within 15 days.It is known micro-
The difference of biological organic fertilizer and organic fertilizer is that rush caused by the metabolic activity of bacteria containing amount and these microorganisms in soil
Raw production-increasing function.General fermenting and producing microbial organic fertilizer all undergoes pyroprocess（60-70℃）Although starter bacteria content
Height, but thalline mortality in pyroprocess, the bacterial content after the completion of fermentation in fertilizer be not high.And ferment and complete in the present invention
Total bacterial content is high in microbial organic fertilizer afterwards（≥5*109cfu/g）, far super general microbial organic fertilizer；Microbial organic fertilizer
In beneficial microbe it is very active in soil, apply the soil of microbial organic fertilizer compared with other experimental groups, organic matter,
Alkali-hydrolyzable nitrogen and quick-acting potassium content significantly improve, and especially quick-acting potassium content improves 1 times than other experimental groups, illustrates that this applies micro- life
Organic fertilizer is improved the special efficacy of soil effective K content.
Script is used for the delicate fragrance type wine brewing Daqu of fermentation alcohol by the present invention using cereal crops such as sorghums as fermentation substrate, adds
Add specific beneficial functions microbe groups, using agricultural wastes such as chicken manure, stalk and furfural dregs as fermentation substrate, change fermentation bar
Part, fermentation are made the microbial organic fertilizer containing a large amount of beneficial functions microorganisms, growth-promoting obvious effect of increasing production are applied in field.This
The prepared microbial organic fertilizer of invention is applied widely, can be applied in the growth-promoting volume increase of fruits and vegetables, crops, nursery stock etc.,
It is respectively provided with good effect of increasing production.The present invention applies special composite ferment by animal wastes, the discarded object of agricultural, life rubbish
Rubbish etc., changes into the green fertilizer rich in beneficial microbe and organic matter, and application prospect is had an optimistic view of.
Brief description of the drawings
Fig. 1 is the horizontal composition testing result figure of delicate fragrance type wine brewing Daqu Bacteriophyta；Fig. 2 is delicate fragrance type wine brewing Daqu Eumycota
Level composition testing result figure；Fig. 3 is the horizontal composition testing result figure of delicate fragrance type wine brewing Daqu bacterium category；Fig. 4 makes for delicate fragrance type
The horizontal composition testing result figure of wine Daqu fungi；Fig. 5 is that the growth-promoting prepared by the present invention increases production microbial organic fertilizer Bacteriophyta
Level composition testing result figure；Fig. 6 is that growth-promoting increases production the horizontal composition testing result figure of microbial organic fertilizer Eumycota；Fig. 7 is rush
The raw horizontal composition testing result figure of volume increase microbial organic fertilizer bacterium category；Fig. 8 is that growth-promoting increases production microbial organic fertilizer fungi water
Flat composition testing result figure；Fig. 9 is bacterial content curve map during fermentation；Figure 10 is three kinds of Daqu different ratio testing inspection results
Figure.
Embodiment
Embodiment 1：A kind of growth-promoting increases production microbial organic fertilizer, and the percentage by weight of each raw material is：60% decomposed chicken
Excrement, 25% powder of straw, 5% wheat bran and 10% furfural dregs are mixed into fermentation substrate, by fermentation substrate weight 0.5% connect bacterium amount be inoculated with into
Composite bacteria agent；The composite bacteria agent is by volume：10% bacillus amyloliquefaciens（Bacillus amyloliquefaciens）, 20% Kang Shi pseudomonads（Pseudomonas costantinii）, 20% Trichoderma harzianum
（Trichoderma harzianum）With the wine brewing Daqu mixing of 50% delicate fragrance type.Delicate fragrance type wine brewing Daqu is the red heart of delicate fragrance type wine
Bent bent and rear fire is 1 by weight：The mixed starters of 2 mixing.
Growth-promoting increases production the specific fermentation process of microbial organic fertilizer：It is inoculated with fermentation substrate into composite bacteria agent, then by water
Point regulation is to 50%-55%, and heap pile in echelon, it is 40-45 DEG C control fermentation temperature, and fermentation 15 days, then spreading for cooling is air-dried to aqueous
Amount≤15%, bacteria containing amount >=5*109Cfu/g.Trapezoidal pile base heap is wide 3 meters, and top heap is wide 1 meter, and heap is high 1 meter.
In composite bacteria agent：Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）With Kang Shi pseudomonads
（Pseudomonas costantinii）Bacteria containing amount >=4*109Cfu/g, Trichoderma harzianum（Trichoderma harzianum）
Bacteria containing amount >=3*108cfu/g。
Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）, Kang Shi pseudomonads（Pseudomonas costantinii）And Trichoderma harzianum（Trichoderma harzianum）According to inclined-plane culture, seed culture, liquid state fermentation three
Prepared by level culture, specific preparation method is：Bacillus amyloliquefaciens use beef-protein medium with Kang Shi pseudomonads
Culture, cultivation temperature are 25-30 DEG C, primary inclined plane culture 48 hours；The bacillus amyloliquefaciens of inclined-plane culture or Kang Shi are false
Monad is inoculated into 150r/min in fluid nutrient medium, and seed liquor is made in 24 hours in 25-30 DEG C of culture；By seed liquor by volume
It is inoculated in for 1%-5% inoculum concentration in the fermentation tank of the beef extract-peptone fluid nutrient medium equipped with sterilizing, tank internal pressure is 0.02-
0.04MPa, 37 DEG C, 1.8 ± 0.2mg/L of dissolved oxygen, rotating speed 150r/min of temperature, bacillus amyloliquefaciens incubation time are 36-
40 hours, Kang Shi pseudomonads incubation time was 40-48 hours；Trichoderma harzianum PDA culture medium culture, cultivation temperature are
28-32 DEG C, primary inclined plane culture 48 hours；The Trichoderma harzianum of inclined-plane culture is inoculated into 180r/min in fluid nutrient medium, 28-
Seed liquor is made in 24 hours in 32 DEG C of cultures；Seed liquor is inoculated in the PDA liquid equipped with sterilizing for 1%-5% inoculum concentration by volume
In the fermentation tank of body culture medium, tank internal pressure is 0.02-0.04MPa, 37 DEG C, 2.0 ± 0.2mg/L of dissolved oxygen, rotating speed 180r/ of temperature
Min, incubation time are 44-48 hours.
Test example 1：Three kinds of Daqu different ratio experiments.4 groups of different ratios of experiment point are carried out, and are respectively：A：Clear stubble is bent：It is red
Innermost being：Fire song=1 afterwards：1：1；B：Red heart is bent：Fire song=1 afterwards：1；C：Red heart is bent：Fire song=1 afterwards：2；D：Red heart is bent：Fire song=1 afterwards：
3.60% decomposed chicken manure, 25% powder of straw, 5% wheat bran and 10% furfural dregs are mixed into fermentation substrate, by fermentation substrate weight
0.5% bacterium amount that connects is inoculated with 4 groups of different leavenings；Then by moisture regulation to 50%-55%, 4 trapezoidal piles, control hair are piled
Ferment temperature is 40-45 DEG C, is fermented 15 days, then spreading for cooling is air-dried to water content≤15%.Trapezoidal pile base heap is wide 3 meters, and top heap is wide
1 meter, heap is high 1 meter.Using flat board gradient dilution method, 1,3,5 ... ..13 in fermentation process, organic mixture is detected respectively within 15 days
Bacterial content in material.As shown in Figure 10, after the completion of fermentation, experimental group C（Red heart is bent：Fire song=1 afterwards：2）Bacteria containing amount is maximum, reaches
4.86×109cfu/g.Thereby determine that, Daqu optimal proportion is that red heart is bent：Fire song=1 afterwards：2.
Test example 2：Bacteria detection and bacterium in growth-promoting volume increase microbial organic fertilizer after the completion of fermentation in delicate fragrance type wine brewing Daqu
The detection of group's composition, delicate fragrance type wine brewing Daqu（Red heart is bent：Fire song=1 afterwards：2）In Flora distribution：Measured using genome high pass
Flora composition and distribution in sequence technical Analysis Daqu.
1. the extraction of genomic DNA：Genomic DNA using bacterium and fungal genomic DNA extracts kit to sample
Extracted, detect DNA purity and concentration using agarose gel electrophoresis afterwards, take appropriate sample to make in centrifuge tube
With sterilized water dilute sample to 1ng/ μ l.
2.PCR is expanded：Genomic DNA after dilution is template；According to the selection in sequencing region, using with Barcode's
Special primer；Use the Phusion High-Fidelity PCR Master Mix of New England Biolabs companies
with GC Buffer.Enter performing PCR using efficient and high-fidelity enzyme, it is ensured that amplification efficiency and accuracy.Primer corresponding region：
16S V4 areas primer is 515F-806R；ITS1 areas primer is ITS1F- ITS2；ITS2 areas primer is：ITS2-3F-- ITS2-
4R。
The sample mixing of 3.PCR products and purifying：PCR primer carries out electrophoresis detection using the Ago-Gel of 2% concentration；According to
PCR primer concentration carries out isoconcentration sample mixing, detects PCR primer using 2% agarose gel electrophoresis after fully mixing, uses
The GeneJET glue reclaim kit recovery products of Thermo Scientific companies.
4. library construction and the sequencing of upper machine：Use the NEB Next Ultra of New England Biolabs companies
DNA Library Prep Kit for Illumina build the structure that storehouse kit carries out library, and the library built is passed through
Qubit is quantified and library detection, and after qualified, upper machine sequencing is carried out using MiSeq.
5. data message is analyzed：Obtained initial data (Raw Data) is sequenced, a certain proportion of interference data be present
(Dirty Data), in order that the result of information analysis is more accurate, reliable, initial data is spliced first, filtered, is obtained
To valid data (Clean Data).It is then based on valid data and carries out OTUs (Operational Taxonomic Units
) cluster, then carry out the species taxonomy analysis in sample room cluster and each sample.Analysis exhibition is made in door level and category level
Show, bacterium, Eumycota in delicate fragrance type wine brewing Daqu, the horizontal result of category are shown in Fig. 1 to Fig. 4, and growth-promoting increases production micro- life after the completion of fermentation
The horizontal result of door, category of bacterium, fungi in organic fertilizer is shown in Fig. 5 to Fig. 8, from experimental result, growth-promoting volume increase microorganism
After the completion of organic fertilizer fermentation, the beneficial functions Pseudomonas amount reproduction such as bacillus, pseudomonas, trichoderma and aspergillus accounts for
According to superiority, and the dominant bacteria Candida content of script falls sharply, and course of fermentation is directed change.
Test example 3：Using flat board gradient dilution method, the bacterial content increased production to growth-promoting in microbial organic fertilizer fermentation process becomes
Change is detected.1,3,5 ... ..13,15 days bacterial contents detected respectively in organic mixture material in fermentation process.Experiment knot
Fruit sees Fig. 9, and as seen from the figure, delicate fragrance type wine brewing Daqu composite bacteria agent is bred rapidly during the fermentation, bacterial content fast lifting, hair
5.31*10 is reached after the completion of ferment9cfu/g。
Test example 4：Increase production the application effect of microbial organic fertilizer for detection growth-promoting, carry out quality and yield to having a competition
Test, using watermelon as studying thing（Western No. 4 kinds of agriculture）, the watermelon seed of full seed is chosen, with 10% hydrogen peroxide surface sterilization
5min, 55 DEG C of hot water treatments are soaked 8 hours to excite seed vitality, are subsequently placed at 30 DEG C and protect for 30 minutes at ambient temperature
Warm vernalization.After seed shows money or valuables one carries unintentionally, choose the consistent seed of germination degree and be sown into quartz sand, moved after seedling grows 2 true leaves
Plant big Tanaka.
Experiment is divided into 3 groups, control group（CK）It is not added with any fertilizer；Experimental group 1（BOF）Apply the present invention by 1000kg/ mus
Prepared growth-promoting volume increase microbial organic fertilizer；Experimental group 2（OF）Apply the natural Chicken dung of equivalent.Every group of 3 repetitions, often
Individual 0.5 mu of repetition, 700 seedlings of planting density/mu.Investigation center pol, edge pol, vitamin C and solubility after watermelon harvest
The 4 big index of quality of albumen, conversion per mu yield investigation yield index.
The quality of watermelon yield application form of table 1
| Processing | Center pol (" BX) | Edge pol (" BX) | Vitamin C（mg·kg-1） | Soluble protein（mg·kg-1） | Per mu yield（kg） |
| CK | 8.01±0.15c | 7.08±0.32b | 145±3 | 211±2c | 3623±17c |
| BOF | 11.48±0.23a | 9.76±0.29a | 174±3a | 269±5a | 4203±11a |
| OF | 9.25±0.31b | 7.34±0.14b | 153±2b | 243±4b | 3999±25b |
Note：In same row, significant difference (p between different alphabetical expression processing<0.05).Similarly hereinafter.
As shown in Table 1, the 4 big index of quality of watermelon are all remarkably higher than control group after application growth-promoting volume increase microbial organic fertilizer
With chicken manure group, per mu yield improves 580 kilograms than control, improves 204 kilograms than applying chicken manure, growth-promoting volume increase microbial organic fertilizer growth-promoting
Obvious effect of increasing production.
Test example 5：Rhizosphere soil sample, every part of 100g, each experimental group are taken in experimental group of the harvest time in test example 4
5 parts are taken, detection soil pH, organic matter, alkali-hydrolyzable nitrogen, available phosphorus, the content of effective these indexs of potassium.
The soil physico-chemical property application form of table 2
| Processing | pH | Organic matter (g kg−1) | Alkali-hydrolyzable nitrogen (mg kg−1) | Available phosphorus (mg kg−1) | Available potassium (mg kg−1) |
| Ck | 6.64 ± 0.05a | 14.59 ± 0.27b | 24.50 ± 1.48c | 37.03 ± 0.91b | 241.06 ± 3.09c |
| BOF | 6.71 ± 0.04a | 19.05 ± 0.52a | 59.43 ± 1.86a | 51.63 ± 1.68a | 512.37 ± 2.26a |
| OF | 6.67 ± 0.03a | 18.87 ± 0.43a | 38.72 ± 1.23b | 49.54 ± 0.72a | 259.74 ± 2.64b |
As shown in Table 2, after increasing production microbial organic fertilizer using growth-promoting, organic matter, alkali-hydrolyzable nitrogen, available phosphorus and available potassium in soil
Content is all improved, and especially quick-acting potassium content is higher by 1 times than other experimental groups, and it is organic to illustrate that this applies growth-promoting volume increase microorganism
Fertilizer is improved the special efficacy of soil effective K content.
Test example 6：Using cucumber as studying thing（Double excellent kings）, the application effect of detection growth-promoting volume increase microbial organic fertilizer,
Quality and yield contrast test is carried out：The cucumber seeds of full seed are chosen, with 10% hydrogen peroxide surface sterilization 5min, 45 DEG C
Hot water treatment is soaked 4 hours to excite seed vitality, is subsequently placed at 30 DEG C and is incubated vernalization for 20 minutes at ambient temperature.Treat
After seed shows money or valuables one carries unintentionally, choose the consistent seed of germination degree and be sown into quartz sand, grow to " page four are wholeheartedly " after seedling and be transplanted to after the phase
Big Tanaka.
Experiment is divided into 3 groups, control group（CK）It is not added with any fertilizer；Experimental group 1（BOF）Apply the present invention by 2000kg/ mus
Prepared growth-promoting volume increase microbial organic fertilizer；Experimental group 2（OF）Apply the natural Chicken dung of equivalent.Every group of 3 repetitions, often
Individual 0.5 mu, spacing in the rows 25cm, line-spacing 60cm of repetition.Fruit is investigated after watermelon harvest to indulge footpath, fruit transverse diameter, single melon quality refers to again etc.
Mark, conversion per mu yield investigation yield index.
The cucumber quality yield application form of table 3
As shown in Table 3, the index of quality of cucumber is all remarkably higher than control group and chicken manure after application growth-promoting volume increase microbial organic fertilizer
Group, per mu yield improve 662 kilograms than control, improve 424 kilograms than applying chicken manure, microbial organic fertilizer growth-promoting obvious effect of increasing production.
Test example 7：Using 5 years in the orchard of Shanxi Province Wanrong County raw Fuji apples as studying thing, detection growth-promoting volume increase microorganism
The application effect of organic fertilizer, experiment are divided into 3 groups, control group（CK）It is not added with any fertilizer；Experimental group 1（BOF）Applied by 4000kg/ mus
The growth-promoting prepared by the present invention is added to increase production microbial organic fertilizer；Experimental group 2（OF）Apply the natural Chicken dung of equivalent.Every group 3
Individual repetition, it is each to repeat 0.5 mu, the index of quality such as pol, vitamin C and titratable acid are investigated after apple harvest, convert per mu yield
Investigate yield index.
The apple quality yield application form of table 4
| Processing | Pol (" BX) | Vitamin C（mg·kg-1） | Titratable acid（%） | Per mu yield（kg） |
| CK | 11.55±0.34c | 131±2 | 0.30±0.04c | 1581±25c |
| BOF | 14.30±0.87a | 154±6a | 0.45±0.03a | 1932±77a |
| OF | 12.97±0.99b | 143±2b | 0.43±0.07b | 1804±14b |
As shown in Table 4, the index of quality of apple is all remarkably higher than control group and chicken manure after application growth-promoting volume increase microbial organic fertilizer
Group, per mu yield improve 351 kilograms than control, improve 128 kilograms than applying chicken manure, the growth-promoting volume increase of growth-promoting volume increase microbial organic fertilizer
Significant effect.
Claims (6)
1. a kind of growth-promoting increases production microbial organic fertilizer, it is characterised in that：The percentage by weight of each raw material is：60% decomposed chicken
Excrement, 25% powder of straw, 5% wheat bran and 10% furfural dregs are mixed into fermentation substrate, by fermentation substrate weight 0.5% connect bacterium amount be inoculated with into
Composite bacteria agent；The composite bacteria agent is by volume：10% bacillus amyloliquefaciens（Bacillus amyloliquefaciens）, 20% Kang Shi pseudomonads（Pseudomonas costantinii）, 20% Trichoderma harzianum
（Trichoderma harzianum）With the wine brewing Daqu mixing of 50% delicate fragrance type.
A kind of 2. growth-promoting volume increase microbial organic fertilizer according to claim 1, it is characterised in that：The delicate fragrance type wine brewing is big
Song is that the bent and rear fiery song of the red heart of delicate fragrance type white spirit by solid state method is 1 by weight：The mixed starters of 2 mixing.
A kind of 3. growth-promoting volume increase microbial organic fertilizer according to claim 1 or 2, it is characterised in that：New micro- life
The specific fermentation process of organic fertilizer is：It is inoculated with fermentation substrate into composite bacteria agent, then by moisture regulation to 50%-55%, piles
Trapezoidal pile, it is 40-45 DEG C to control fermentation temperature, is fermented 15 days, then spreading for cooling is air-dried to water content≤15%, bacteria containing amount >=5*
109Cfu/g.
A kind of 4. growth-promoting volume increase microbial organic fertilizer according to claim 3, it is characterised in that：In the composite bacteria agent：
Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）With Kang Shi pseudomonads（Pseudomonas costantinii）Bacteria containing amount >=4*109Cfu/g, Trichoderma harzianum（Trichoderma harzianum）Bacteria containing amount >=3*
108cfu/g。
A kind of 5. growth-promoting volume increase microbial organic fertilizer according to claim 4, it is characterised in that：The solution starch gemma bar
Bacterium（Bacillus amyloliquefaciens）, Kang Shi pseudomonads（Pseudomonas costantinii）It is wooden with thatch is breathed out
It is mould（Trichoderma harzianum）Prepared according to inclined-plane culture, seed culture, liquid state fermentation third stage culture, it is specific to prepare
Method is：Bacillus amyloliquefaciens use beef-protein medium culture, cultivation temperature 25-30 with Kang Shi pseudomonads
DEG C, primary inclined plane culture 48 hours；The bacillus amyloliquefaciens of inclined-plane culture or Kang Shi pseudomonads are inoculated into Liquid Culture
Seed liquor is made in 24 hours in 150r/min in base, 25-30 DEG C of culture；Seed liquor is inoculated with for 1%-5% inoculum concentration by volume
In the fermentation tank of the beef extract-peptone fluid nutrient medium equipped with sterilizing, tank internal pressure is 0.02-0.04MPa, and 37 DEG C of temperature is molten
1.8 ± 0.2mg/L of oxygen is solved, rotating speed 150r/min, bacillus amyloliquefaciens incubation time is 36-40 hours, Kang Shi pseudomonads
Incubation time is 40-48 hours；Trichoderma harzianum PDA culture medium culture, cultivation temperature are 28-32 DEG C, primary inclined plane training
Support 48 hours；The Trichoderma harzianum of inclined-plane culture is inoculated into 180r/min in fluid nutrient medium, 28-32 DEG C of culture is made for 24 hours
Seed liquor；Seed liquor is inoculated in the fermentation tank of the PDA liquid medium equipped with sterilizing for 1%-5% inoculum concentration by volume
In, tank internal pressure is 0.02-0.04MPa, 37 DEG C, 2.0 ± 0.2mg/L of dissolved oxygen, rotating speed 180r/min of temperature, and incubation time is
44-48 hours.
A kind of 6. growth-promoting volume increase microbial organic fertilizer according to claim 3, it is characterised in that：The trapezoidal pile base heap
Wide 3 meters, top heap is wide 1 meter, and heap is high 1 meter.
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