CN105130671A - Preparation and application methods for novel multi-resistant compound microorganism fertilizer for preventing soil-borne diseases of watermelons and muskmelons - Google Patents
Preparation and application methods for novel multi-resistant compound microorganism fertilizer for preventing soil-borne diseases of watermelons and muskmelons Download PDFInfo
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- Fertilizers (AREA)
Abstract
The invention relates to preparation and application methods for a novel multi-resistant compound microorganism fertilizer for preventing soil-borne diseases of watermelons and muskmelons, which belong to the field of fertilizer production. According to the preparation and application methods, carbon-containing plant powder and protein grain powder are added into organic fertilizer compost to prepare an organic material; functional bacteria are added into the organic material; the novel multi-resistant compound microorganism fertilizer is obtained by mixing fully and uniformly. The functional bacteria of the novel multi-resistant compound microorganism fertilizer multiply massively in the rhizosphere of melon crops to form a circular protective cover and can effectively resist the diseases caused by a variety of fungi; a good ecological environment is created for the growth of the watermelons and the muskmelons; the soil environment can be improved; the hardening can be broken; the permeability and oxygen supply amount of soil are improved; the product quality and yield are improved; double effects of a fertilizer and a pesticide are achieved. The fertilizer contains greater than or equal to 50 percent of organic matter, and each gram of the fertilizer contains 500 million beneficial bacteria; the beneficial bacteria belong to the hyphomycetes of the deuteromycotina and cannot generate mutual antagonism. The fertilizer is convenient to apply and high in fertilizer efficiency, requires use for only one time, is labor-saving, and does not pollute the environment.
Description
technical field:
The present invention relates to a kind of novel how anti-composite microbe fertilizer preparations and applicatio method of preventing west, muskmelon soil-borne disease, belong to field of fertilizer manufacturing.
background technology:
Existing biological organic fertilizer, microorganism fertilizer, the function yeast of adding pre-disease prevention is all generally 5406 bacterium or subtilis, and the hormone produced by bacterial classification suppresses pathogenic bacteria to occur.Amount of application is few, and fertilizing method is not right, and in matrix, albumen lacks, and effective supply can not have function yeast flourish, and its effect is not too obvious, particularly soil-borne disease.As: blight, root knot nematode disease, almost can't see effect.
Summary of the invention
An object of the present invention is to provide a kind of can intercept infecting and attacking of pathogenic bacteria around west, muskmelon offspring, simultaneously for improve protein content in organic matter, provide nutrition to growth, reach fertile, a kind of medicine economic benefits and social benefits object novel how anti-composite microbe fertilizer preparations and applicatio method of preventing west, muskmelon soil-borne disease.
Technical solution
A kind of novel how anti-composite microbe fertilizer preventing west, muskmelon soil-borne disease, it is characterized in that, preparation method is as follows: in fertilizer becomes thoroughly decomposed, add carbon containing Plant Powder and protein-contg cereal powder ferments, carbon-nitrogen ratio 22-28:1, add function yeast again in organic materials after fermentation, fully mix and obtain how anti-composite microbe fertilizer.
Described fertilizer becomes thoroughly decomposed employing animal excreta, comprises one or two or more kinds mixing in sheep excrement, cow dung, chicken manure.
Described carbon containing Plant Powder comprises: straw powder, furfural dregs, sweet sorghum slag.
Described cereal powder comprises one or two or more kinds mixing in Semen Maydis powder, oil cake or soya-bean cake.
Described function yeast comprises: content reach 45-55 hundred million/gram trichoderma viride, content reach 45-55 hundred million/gram trichoderma harzianum, content reach 45-55 hundred million/gram Paecilomyces lilacinus 4%.
The present invention, for accelerating organic materials fermenting speed, adds the decomposing agent of 1.5-3% in organic materials.
Material of the present invention is all weight percentage, and fertilizer becomes thoroughly decomposed for 40-60% sheep excrement and 15-25% chicken manure; Carbon Plant Powder is 15-25% straw powder, and protein-contg cereal powder is 4-6% Semen Maydis powder, 2-4% oil cake; Also comprise decomposing agent 1.5-3 and content reach 45-55 hundred million/gram trichoderma viride 2-3%, content reach 45-55 hundred million/gram trichoderma harzianum 3.5-4.5%, content reach 45-55 hundred million/gram Paecilomyces lilacinus 3.5-4.5%.
Prevent the preparation method of novel how anti-composite microbe fertilizer for west, muskmelon soil-borne disease, it is characterized in that, preparation method is as follows:
1) preparation of organic materials is by 40-60% sheep excrement, 15-25% chicken manure, 15-25% straw powder, 4-6% Semen Maydis powder, 2-4% oil cake, 1.5-3% fertilizer decomposing agent, fully mix, in temperature: compost under the high temperature of 55-60 DEG C, kill pathogenic bacteria, when stacking subsides, be covered with white hypha, odorless, reaches innoxious standard, namely makes organic materials;
2) in 85-95% organic materials, add function bacterial classification, the add-on of function bacterial classification accounts for the 10-12% of organic materials total amount; Fully mix, namely make how anti-composite microbe fertilizer; Wherein: probiotics comprises: content reach 45-55 hundred million/gram trichoderma viride 2-3%, content reach 45-55 hundred million/gram trichoderma harzianum 3.5-4.5%, content reach 45-55 hundred million/gram Paecilomyces lilacinus 3.5-4.5%.
Prevent the application method of novel how anti-composite microbe fertilizer for west, muskmelon soil-borne disease, it is characterized in that, adopt plane pothole to execute and spread fertilizer over the fields with ridging; mix with soil; amount reproduction function bacterial classification around the root circle of the every seedling of western muskmelon, forms protective guard, the invasion and attack of opposing pathogenic bacteria.
Positive beneficial effect of the present invention
Describe pest controlling principle of work after bacterial classification compound of the present invention: viride, trichoderma harziarum, Paecilomyces lilacinus all belong to Deuteromycotina, hyphomycetes, hyphomycetales, mutual not antagonism after mixing, the microbiotic of secretion can suppress growth of pathogenic bacteria, field planting.
Trichoderma harziarum and Paecilomyces lilacinus bacterial classification can superparasitism on host bacterium, decompose the cell walls of pathogenic bacteria, penetrate host mycelia, draw nutrition.Killed by pathogenic bacteria and energy Parallel Growth and spiral winding, make root of the crop circle form protective guard, any pathogenic bacteria can not infect and attack, and particularly has toxic action to root knot nematode.Test-results shows: can effectively prevent root knot nematode disease, root rot, damping-off, blight, sclerotium disease, gray mold, anthrax, have how anti-effect.
Three kinds of bacterium compounds also can improve soil, abolish and harden, and improve soil permeability and oxygen-supplying amount, promote crop root and plant strain growth, also have promoter action to seed germination growth.Wheat seeding seedling is neat, Miao Wang, Miao Zhuan.Three bacterium compounds, have fertile medicine double effects.
Fertilizer, as matrix, adds proteinogen, accelerates culture propagation growth.
Facts have proved, technique not only can prevent soil-borne disease, and anti-continuous cropping successful.
Instant invention overcomes the defect of prior art, have the ability of anti-multiple diseases, to root knot nematode disease, blight, root rot, damping-off control efficiency more than 80%, has desinsection, disease resistance, double effects.Use conveniently, fertilizer efficiency is high, uses and only needs once, saves labour, free from environmental pollution.
specific implementation method:
(2) probiotics adds viride 2%, and content reaches 5,000,000,000/gram, trichoderma harzianum adds 4%, and content reaches 5,000,000,000/gram, Paecilomyces lilacinus 3%, content reaches 5,000,000,000/gram.
Fertilizer of the present invention becomes thoroughly decomposed, by sheep excrement 50%, chicken manure 20%, straw powder 20%, with Semen Maydis powder 5%, oily Pie 3% carbon-nitrogen ratio 25:1; With decomposing agent 2%, in order to accelerate fermentation, above-mentioned material, abundant premix, compost fermentation, temperature is raised to 55 DEG C-60 DEG C, turning every day once, is thoroughly become thoroughly decomposed, is killed all bacteriums in ight soil and roundworm egg for 15-20 days, and (namely stacking subsides to reach the requirement of harmless treatment, be covered with white hypha, odorless).Latter stage of ripening, retains 7-10 days, as the carrier of function yeast, completes.Add Semen Maydis powder and oily Pie in organic fertilizer fermentation process of the present invention, object is that the function yeast supplied afterwards is grown.
Add function yeast: in the fertilizer of latter stage of ripening, add trichoderma viride 2%(content reach 5,000,000,000/gram), trichoderma harzianum 4%(content reaches 5,000,000,000/gram), Paecilomyces lilacinus 3%(content reaches 5,000,000,000/gram), fully mixing, make the novel how anti-composite microbe fertilizer of the prevention western muskmelon soil-borne disease prepared, function yeast total content reaches 500,000,000/gram, organic content >=50%, moisture≤30%, is chemically examined by quality inspection, qualified product packaging warehouse-in.
Bacterial classification morphological structure, mechanisms of control wheat scab and effect describe: viride Pseudomonas Deuteromycotina, hyphomycetes, hyphomycetales, sticky spore mushroom, can produce multifunction activity enzyme, as cellulase, chitinase, the small molecules microbiotic that its produces and macromole antibacterial protein or cell wall degradation enzyme suppress the growth of pathogenic bacteria, and breeding, plays an important role to control of plant disease.
Trichoderma harziarum belongs to Moniliaceae, Deuteromycotina, hyphomycetes, hyphomycetales.Energy Secretases and antibiotic substance, decompose the cell walls of pathogenic bacteria.Form protective guard at roots of plants circle, it has parasitization, and host mycelia secretory product makes trichoderma harziarum tend to host's bacteria growing.Once host is by the identification of trichoderma harziarum parasite, trichoderma harziarum grows along host mycelia Parallel Growth and spiral wound and produces appressorium shape branch and is adsorbed on host mycelia.By secretion extracellular enzyme dissolved cell wall, penetrate host mycelia and draw nutrition, pathogenic bacteria is killed; Secondly the microbiotic of secretion also can suppress growth of pathogenic bacteria field planting, alleviates harm.Be used for preventing pythium spp, dry thread Pyrenomycetes, sickle-like bacteria, the pathogen of Botrytis cinerea successful.
Paecilomyces lilacinus hyphomycete section, deuteromycetes, Hyphomycetales, can colonize in the egg capsule of root knot nematode, and in adhesive matrix, biological and ecological methods to prevent plant disease, pests, and erosion mycelia surrounds whole ovum.Due to the activity of exogenous metabolism thing and chitinase, broken in chorion top layer, fungi invades and replaces subsequently.Also can excrete poison and play toxic action to nematode, its effect in pest controlling is remarkable, and diseases prevention rate can reach more than 80%, also has and anti-ly kills effect, have obvious restraining effect, can stimulate plant growth simultaneously to phytopathogen other insect.
the present inventionaccording to the coagulation efficiency and mechanism of three kinds of beneficial functions bacterium, application requiring:
The first, spread manuer in holes.Before sowing, plane nest spreads fertilizer over the fields and puddles with soil, then sows.Such strain growth is grown, and destroys and kills around pathogenic bacteria and insect, make to form protective guard around root of the crop circle, create good conditions to plant growth.Mu consumption 50-80 kilogram.
Second: warmhouse booth is used, ridging spreads fertilizer over the fields with mixing with soil even, mu consumption 100-200 kilogram, and then mulch sowing forms protective guard in such Neng Shilong road.
The cultivation of trichoderma viride
1. one-level test tube slant is cultivated: PDA substratum: peeled potatoes 200g, and glucose 15g, agar 15g, distilled water 1000mL, be inoculated on test tube PDA substratum by trichoderma viride, activation culture 4 days under 28oC, obtains test tube strains.
2. secondary seed is cultivated: PDB substratum: peeled potatoes 200g, glucose 15g, distilled water 1000mL, access in PDB substratum by test tube strains, shaking table is cultivated 4 days (28oC, 140 revs/min) and made seed liquor.
3. liquid fermentation and culture: after fermentation equipment and medium sterilization, by the volume ratio of secondary seed solution 1:10 inoculation PDB fermentation culture, 28oC bottom fermentation 72 hours, tank pressure 0.05MPa; Air flow 6Slpm; Stirring velocity 150rpm; Sampling in every 10 hours once, is observed with or without miscellaneous bacteria, detects viable count reach (2.5--3.0) × 10 with culture medium flat plate method
8ge ∕ mL.The sterilizing of pipeline, air filter, fermentor tank, substratum is undertaken by production technique standard.
The cultivation of trichoderma harzianum strain
Bacterial strain FJAT-9040 are provided by the Chinese Academy of Agricultural Sciences.With potato, sucrose, agar, PDA substratum, liquid fermenting CheckShi substratum, SODIUMNITRATE 3g, potassium primary phosphate 1g, magnesium sulfate 0.5g, Repone K 0.5g, ferrous sulfate 0.01g, sucrose 30g, water 1000mL.
In CheckShi substratum, (liquid amount is 50mL or 250mL triangular flask 28oC120rmin in bacterial classification FJAT-9040 of inoculation activation
-1shaking culture 96h, nutrient solution is crossed after elimination mycelium through sterile gauze, for subsequent use as seed liquor)
Preparation Cha Shi liquid nutrient medium 30L, join 50L fermentor tank, follow procedure is cultivated, sterilizing 30min, cooling 28oC, by seed liquor by 1% inoculum size access fermentor tank, tank temperature 28oC, air flow 0.8vvm, rotating speed 120r.min
-1, carry out fermentation culture.Sample 300mL every 8h, 12h, 24h, be divided into three parts, use fermentation state parametric measurement, measure spore count by dilution coated plate method.
The cultivation of Paecilomyces lilacinus
Bacterial strain is provided by the Chinese Academy of Agricultural Sciences.With PDA or CMA substratum breeding (potato, sucrose medium), incubation time 5d.
Liquid fermenting, with seeding tank fermentation, culture temperature 28oC, tank pressure 0.05-0.01Mpa, rotating speed 200-250r.min
-1, ventilation 1:0.5, cultivates 24-48h.
Fermentor cultivation 62-72h, index 0.5mgmL
-1, every 6h sampling once, inspection is without miscellaneous bacteria, and mycelia big area networks, biomass less than 12%.Mensuration bacterial content is qualified.
advantage of the present invention
1. under using the heavy metal pollution of soil and agricultural product quality that chemical fertilizer brings in recent years in a large number, degradation negative impact was more and more serious, the invention belongs to microbial fertilizer, heavy metal content of can degrading, improve quality of agricultural product, being environmental friendly fertilizer, is the first-selection of producing Organic food.
2. the using of a large amount of agricultural chemicals, makes various disease and pest resistance strengthen, some soil disinfection organophosphorus pesticide, has made many people eat vegetables.Facts have proved have many vegetables organophosphoruss to exceed standard, the present invention can degrading pesticide residues, and suppress multiple pathogenic bacteria to occur and the generation of root knot nematode disease, experimental result shows that control rate reaches 80%, the excellent way of real genus biological control soil-borne disease.Although it is strict to join fertile fertilizing method requirement, effect is fine, has fertile medicine double effects.
3. the present invention with the addition of Semen Maydis powder in composting fermentation process, and oily Pie, adds protein source, grows to the function yeast of adding afterwards and provides grain.
4. the present invention has and increases soil fertility, and improves soil permeability and oxygen delivery capacity, the good actions such as elimination is hardened, preserve moisture and fertility, to promotion crop root growth and seed germination, has the not replaceable effect of other fertilizer.
5. three bacterium compounds, mutually not antagonism, have fertile medicine double effects simultaneously, and be optimal fertilizer in recent years, promotion prospect is very good.According to test measuring and calculating, huge economic and social benefits will be brought.
6. fertilizer practice requires strict, and root of the crop circle really defines protective guard, creates good condition to plant growth.
The present invention has integrated technology, and each link is indispensable, does not see replacement scheme so far.
Key problem in technology point of the present invention and protection point
One is add corn, oily Pie, provides grain to function yeast.
Two is three bacterium compounds, mutually not antagonism.
Three is have fertile medicine double effects.
Four is that fertilizer practice requires strict.
Field application effect test-results is as follows:
One, experiment purpose
Wuyuan County is moistened the novel how anti-composite microbe fertilizer of prevention western muskmelon soil-borne disease that biotechnology limited liability company produces, have and suppress soil surface characters to produce, kill root knot nematode cause of disease bacterium, improvement soil, promotes the effect of crop root growth and seed germination.In order to verify its result of use in west, greenhouse, on muskmelon, we have selected continuous 3 years continuous cropping, root knot nematode disease, root rot, blight, damping-off, the multiple diseases such as gray mold occur very serious, almost can not plant west again, the plot of muskmelon tests, inquire into its protection effect and optimum fertilizing amount, fertilizing method.Special work arranges as follows, provides theoretical foundation for promoting from now on.
Experiment material and place
Trial crops: watermelon (being early preced with dragon), muskmelon (lantern is red)
Test fertilizer: the novel many anti-micro-composite microbe fertilizers of moist board (probiotics 500,000,000/gram, organic > 50%);
West, special melon fertilizer (containing N14, P2O522, K2O12)
Test site: Wuyuan County victory Fengzhen newly red two societies pungent triumph warmhouse booth (autumn and winter stubble)
Three, test method and design
Watermelon is established three process, Gong Sange community, area 205.8 ㎡.Every community 7 film, zone leader 7 meters, wide 9.8 meters, wide row space 90cm, little line-spacing 50cm, spacing in the rows 50cm, area 68.6 ㎡, stays seedling 196 strain, and mu stays seedling 1904 strain.
Muskmelon is established three process, Gong Sange community, area 218.4 ㎡.Every community 8 film, zone leader 7 meters, wide 10.4 meters, wide row space 90cm, little line-spacing 40cm, spacing in the rows 40cm, area 72.8 ㎡, stays seedling 272 strain, and mu stays seedling 2482 strain.
Watermelon: can find out process 1 from table (): general planting, year after year continuous cropping, stay seedling 196 strain altogether, 98 strains of falling ill, sickness rate 50%, morbidity is very serious, almost can not plant watermelon again.
Process 2: behave oneself best plantation 196 strain, 14 strains of falling ill, and sickness rate 7%, prevention rate can reach 93%.It is distinguished that the novel how anti-composite microbe fertilizer of sufficient proof prevents and treats soil-borne disease effect.
Process 3: performance is good, and plant 196 strains, 25 strains of falling ill, sickness rate 13%, prevention rate can reach 87%.Prove that novel how anti-composite microbe fertilizer prevents and treats soil-borne disease obviously, be only second to process 2.Illustrate that ridging spreads fertilizer over the fields good not as the method executed of plane pothole.
Muskmelon: process 1(general planting as can be seen from Table I, continuous cropping year after year) stay seedling 272 strain altogether, 148 strains of falling ill, sickness rate 54%, morbidity is serious, can not plant muskmelon again.
Process 2: behave oneself best plantation 272 strain, 36 strains of falling ill, and sickness rate 13%, prevention rate can reach 87%.It is huge that the novel how anti-composite microbe fertilizer of sufficient proof prevents and treats soil-borne disease effect.
Process 3: performance is good, plants 272 strains, 43 strains of falling ill, sickness rate 16%, prevention rate 84%.Be only second to process 2.Illustrate that how anti-novel composite microbe fertilizer effect be obvious, fertilizing method be noted.
Watermelon, as can be seen from Table II:
Process 1: melon type is not of uniform size, and commodity melon only has 30%, per mu yield 1047 kilograms, and yield poorly, benefit is low, should not plant watermelon again.
Process 2: melon type neat and consistent, commodity rate can reach 90%, and per mu yield 3004 kilograms, output high benefit is high, behaves oneself best.Prove by biological control, watermelon can be planted again.
Process 3: melon type neat and consistent, commodity rate 85%, per mu yield 2489 kilograms, performance is good.
Muskmelon, as can be seen from Table II:
Process 1: melon type is not of uniform size to be caused.Have to obtain dead seedling of not bearing fruit, commodity rate 35%, per mu yield 795Kg, yields poorly, and benefit is low.Unsuitable continuous cropping sowing.
Process 2: melon type neat and consistent, commodity rate 91%, output 1946 kilograms, output is high, high efficiency, behaves oneself best.
Process 3: melon type neat and consistent, commodity rate 85%, output 1888 kilograms, performance is good.Illustrate by biological control soil-borne disease, can also muskmelon be planted.
Four, conclusion
1, at the second crop soil of planting west, muskmelon year after year, enrich novel how anti-composite microbe fertilizer, diseases prevention rate can reach 87%--93%, and effect is extremely obvious, and obtains high-yield and high-efficiency, is worth promoting.
2, fertilizing method is a significant improvement of disease-resistant high yield.For many years, bio-fertilizer protection effect is spread fertilizer over the fields not good.This fertilizer adopts plane pothole to execute and mixes soil evenly, then sows, and substantially increases diseases prevention rate.
3, novel how anti-composite microbe fertilizer bacterial classification is selected rationally, and bacteria containing rate is high, and by digging nest, spreading manuer in holes, function yeast amount reproduction.Heavy responsibilities of government master, in fungal pathogen strain, breaks through cell walls, kills pathogenic bacteria, form protective guard around crop.Create good condition to plant growth, this mechanism is correct.Pre-disease prevention effect is obvious.
Claims (9)
1. the novel how anti-composite microbe fertilizer of prevention west, muskmelon soil-borne disease, it is characterized in that, preparation method is as follows: in fertilizer becomes thoroughly decomposed, add carbon containing Plant Powder and protein-contg cereal powder ferments, carbon-nitrogen ratio 22-28:1, in organic materials, add function yeast again, fully mix and obtain novel how anti-composite microbe fertilizer.
2. the novel how anti-composite microbe fertilizer of prevention west according to claim 1, muskmelon soil-borne disease, it is characterized in that, fertilizer becomes thoroughly decomposed employing animal excreta, comprises one or two or more kinds mixing in sheep excrement, cow dung, chicken manure.
3. the novel how anti-composite microbe fertilizer of prevention west according to claim 1, muskmelon soil-borne disease, it is characterized in that, carbon containing Plant Powder comprises: straw powder or furfural dregs, sweet sorghum slag.
4. the how anti-composite microbe fertilizer of prevention west according to claim 1, muskmelon soil-borne disease, is characterized in that, protein-contg cereal powder comprises one or two or more kinds mixing in Semen Maydis powder, oil cake or soya-bean cake.
5. the novel how anti-composite microbe fertilizer of prevention west according to claim 1, muskmelon soil-borne disease, it is characterized in that, function yeast comprises: content reach 45-55 hundred million/gram trichoderma viride 2%, content reach 45-55 hundred million/gram trichoderma harzianum 4%, content reach 45-55 hundred million/gram Paecilomyces lilacinus 4%.
6. the how anti-composite microbe fertilizer of prevention west according to claim 1, muskmelon soil-borne disease, is characterized in that, for accelerating organic materials fermenting speed, adds the decomposing agent of 1.5-3% in organic materials.
7. the novel how anti-composite microbe fertilizer of prevention west according to claim 1, muskmelon soil-borne disease, it is characterized in that, following materials is all weight percentage, and fertilizer becomes thoroughly decomposed for 40-60% sheep excrement and 15-25% chicken manure; Carbon containing Plant Powder is the protein-contg cereal powder of 15-25% is 4-6% Semen Maydis powder, 2-4% oil cake; Also comprise decomposing agent 1.5-3 and content reach 45-55 hundred million/gram trichoderma viride 2-3%, content reach 45-55 hundred million/gram trichoderma harzianum 3.5-4.5%, content reach 45-55 hundred million/gram Paecilomyces lilacinus 3.5-4.5%.
8. the preparation method of the novel how anti-composite microbe fertilizer of prevention west, muskmelon soil-borne disease, it is characterized in that, preparation method is as follows: 1) preparation of organic materials is by 40-60% sheep excrement, 15-25% chicken manure, 15-25% straw powder, 4-6% Semen Maydis powder, 2-4% oil cake, 1.5-3% fertilizer decomposing agent, fully mixes, in temperature: compost under the high temperature of 55-60 DEG C, kill pathogenic bacteria, when stacking subsides, be covered with white hypha, odorless, reaches innoxious standard, namely makes organic materials;
2) in 85-95% organic materials, add function yeast, the add-on of function bacterial classification accounts for the 10-12% of organic materials total amount; Fully mix, namely make novel how anti-composite microbe fertilizer; Wherein: probiotics comprises: content reach 45-55 hundred million/gram trichoderma viride 2-3%, content reach 45-55 hundred million/gram trichoderma harzianum 3.5-4.5%, content reach 45-55 hundred million/gram Paecilomyces lilacinus 3.5-4.5%.
9. the application method of the novel how anti-composite microbe fertilizer of prevention west, muskmelon soil-borne disease, is characterized in that, adopts plane pothole to execute and spreads fertilizer over the fields with ridging; mix with soil; amount reproduction function bacterial classification around the root circle of the every seedling of western muskmelon, forms protective guard, the invasion and attack of opposing pathogenic bacteria.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105367305A (en) * | 2015-12-24 | 2016-03-02 | 深圳市芭田生态工程股份有限公司 | Compound microbial fertilizer with disease and pest resistance as well as preparation method and application of compound microbial fertilizer |
| CN106221705A (en) * | 2016-08-03 | 2016-12-14 | 南宁绿友农农业科技有限公司 | A kind of disease resistance microbe soil restoration agent and preparation method thereof |
| CN106946626A (en) * | 2017-03-23 | 2017-07-14 | 河南省农业科学院园艺研究所 | Prevent composite microbic bacterial fertilizer of root knot nematode disease and preparation method thereof |
| CN107652027A (en) * | 2017-09-12 | 2018-02-02 | 广州万吉生物科技有限公司 | A kind of complex composition of biological control crop droop and its application |
| CN107778035A (en) * | 2017-11-24 | 2018-03-09 | 山西省农业科学院生物技术研究中心 | A kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof |
| CN108184611A (en) * | 2017-12-28 | 2018-06-22 | 内蒙古润泽源生物科技股份有限公司 | Prevent microorganism seedling medium of soil-borne disease and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724558A (en) * | 2009-12-08 | 2010-06-09 | 营口恒新生物技术开发有限公司 | Disease-resistant compound microbial inoculant and preparation method and application thereof |
| CN104862249A (en) * | 2015-05-07 | 2015-08-26 | 上海坤元环保投资有限公司 | Micro-ecologic preparation for preparing high-sterilization-capability micro-organism bacterial fertilizer and preparation method thereof |
-
2015
- 2015-09-16 CN CN201510584785.6A patent/CN105130671A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724558A (en) * | 2009-12-08 | 2010-06-09 | 营口恒新生物技术开发有限公司 | Disease-resistant compound microbial inoculant and preparation method and application thereof |
| CN104862249A (en) * | 2015-05-07 | 2015-08-26 | 上海坤元环保投资有限公司 | Micro-ecologic preparation for preparing high-sterilization-capability micro-organism bacterial fertilizer and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105367305A (en) * | 2015-12-24 | 2016-03-02 | 深圳市芭田生态工程股份有限公司 | Compound microbial fertilizer with disease and pest resistance as well as preparation method and application of compound microbial fertilizer |
| CN106221705A (en) * | 2016-08-03 | 2016-12-14 | 南宁绿友农农业科技有限公司 | A kind of disease resistance microbe soil restoration agent and preparation method thereof |
| CN106946626A (en) * | 2017-03-23 | 2017-07-14 | 河南省农业科学院园艺研究所 | Prevent composite microbic bacterial fertilizer of root knot nematode disease and preparation method thereof |
| CN107652027A (en) * | 2017-09-12 | 2018-02-02 | 广州万吉生物科技有限公司 | A kind of complex composition of biological control crop droop and its application |
| CN107778035A (en) * | 2017-11-24 | 2018-03-09 | 山西省农业科学院生物技术研究中心 | A kind of growth-promoting volume increase microbial organic fertilizer and preparation method thereof |
| CN108184611A (en) * | 2017-12-28 | 2018-06-22 | 内蒙古润泽源生物科技股份有限公司 | Prevent microorganism seedling medium of soil-borne disease and preparation method thereof |
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