CN107652027A - A kind of complex composition of biological control crop droop and its application - Google Patents
A kind of complex composition of biological control crop droop and its application Download PDFInfo
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- CN107652027A CN107652027A CN201710818873.7A CN201710818873A CN107652027A CN 107652027 A CN107652027 A CN 107652027A CN 201710818873 A CN201710818873 A CN 201710818873A CN 107652027 A CN107652027 A CN 107652027A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention belongs to crop disease control field, and in particular to a kind of complex composition of biological control crop yellow leaf droop and its application.The complex composition of the biological control crop droop of the present invention, including organic fertilizer and strain original powder；The weight ratio of organic fertilizer and strain the original powder is 100:1‑20：1.The various droops crop occurred frequently that the complex composition of the present invention can be used in agricultural planting industry, such as banana, dwarf banana.The complex composition and its prevention and controls cost of the biological control crop droop of the present invention are low, and preventing and treating stability is good, is not likely to produce disease resistance, make full use of the advantage of biological control, prevention effect is improved, the daily management that crop is cultivated, planted is improved, reduces economic loss and cost accounting.
Description
Technical field
The invention belongs to crop disease control field, and in particular to a kind of compounded combination of biological control crop droop
Thing and its application.
Background technology
Banana blight is commonly called as yellowtop, Panama wilt disease, and the growth of early stage disease plant is stunt, or even dead.Growth
Growth is weaker after strain is susceptible, it is impossible to which solid, even if solid can not grow up, the plant of infection is also easily infected by bunchy top virus.
Banana infects this after being ill, and quickly, for diseased plant into geometric progression increase, banana blight is a kind of destructive disease of banana for sprawling.
It is a kind of infectious disease as caused by fungal infection.Pathogen is sickle-like bacteria Fusarium oxysporum, is a kind of soil inhabitant, can in soil
Survive even 20 years as long as several years.
According to for many years our researchs to yellow leaf droop, crop yellow leaf is withered to have many reasons to cause, investigation hair
Existing, soil fail, fertilizer damage and disease are the reason for causing crop yellow leaf to be withered.The destruction of wherein soil causes root system quilt completely
Damage, the basic basic reason withered as crop yellow leaf, in recent years since, due to chemical fertilizer, agricultural chemicals (herbicide, bactericide etc.)
It is a large amount of to use so that soil with organic matter be nearly consumed by totally, have impact on the environment that biological self reproducing is survived in soil so that soil
Original existing microbial ecological balance is broken in earth, and harmful microorganism and insects shortage rival or restraint natural enemy are opened
Begin intrusion crop root, is bred in root, destroys root structure, cause root system to rot.Infected in wound and root activity declines
Under the collective effect of the two harm so that crop root does not absorb nutrition, moisture etc. and directly triggered yellowtop droop
Generation.And parasitize the miniature pest type in soil and cannot get required nutriment from soil, crop tuber class, then
The breach of root system is found from the young root of the new tiller of crop, all newborn root systems of crop has been damaged, has made crop slowly because of water shortage
Point, nutrient and wither, cause crop comprehensively wither, death.
Banana blight has following characteristics：(1), soil passes；(2), high temperature and humidity weather is advantageous to disease infestation and extension；
(3), soil acidity is higher, short texture, more field occurs for subsoil poor permeability and banana nematode, and with carrying disease germs
Soil incubation test tube seedling, be advantageous to the generation of disease；(4), initial stage does not fall ill, and is fallen ill before taking out flower bud.
The sick pathogen can closely be propagated by soil and flowing water, moreover it is possible to be carried out by crop seed allocation and transportation remote
Propagate, therefore disease spread speed is exceedingly fast.Preventing and treating to droop at present mainly by disease-resistant variety of choosing seeds, with reference to cultivation management,
The aggregate measures such as Agro-chemicals control.Seed selection disease-resistant variety, early stage, operation was more complicated, and the cost that need to be put into advance is higher.Chemistry
Chemical control, it is main using medicament seed disinfection and the method for soil chemicals treatment.The long term frequent of chemical agent uses, and can draw
The drug-fast generation of pathogen is played, causes prevention effect undesirable, and Practice for Pesticide Residue in Agricultural Products is exceeded and the wildness of harmful organism,
Also result in the serious problem of society today such as environmental pollution, disruption of ecological balance.Conventional chemical method can prevent and treat droop to a certain extent, but
Its cost is high, effect is poor, toxicity of pesticide is big, and residual is high, and its popularity is limited in the presence of the deficiencies of pollution to environment.Biology is anti-
Tool has that cost is low, without environmental pollution, has broad prospects, at present the biological control microbial inoculum of crop droop using bacterium as
Main, preventing and treating stability is poor, and wilt variability is strong in addition, easily produces disease resistance, cause the practical application of biological control by
To larger limitation.
Reflect, prior art also has larger deficiency, it would be highly desirable to improves.
The content of the invention
In consideration of it, be necessary for it is above-mentioned the problem of provide a kind of biological control crop droop complex composition and its
Using, the advantage of biological control is made full use of, improves prevention effect, improves the daily management that crop is cultivated, planted, reduction
Economic loss and cost accounting.
The object of the invention is achieved through the following technical solutions：
A kind of complex composition of biological control crop droop, including organic fertilizer and strain original powder；The organic fertilizer and
The weight ratio of strain original powder is 100:1-20：1；It is preferred that 100:1.The viable bacteria of function bacterium can reach alternate with organic fertilizer during this ratio
The optimum state of profit altogether.
Further, the applicable scope of the organic fertilizer is wide, can be any commercially available organic fertilizer.
Further, the organic fertilizer moisture content ensures below 10%, is being manured into soil with security function strain original powder
In before keep resting state, the organic matter contained by organic fertilizer reaches more than 45%, humic acid more than 8%.
Further, the strain original powder includes trichoderma viride and bacillus subtilis；Trichoderma viride and withered grass bud
The mass ratio of spore bacillus is 7:3-3:7；It is preferred that 1:1.
Further, the strain original powder includes the strain component of following mass ratio：1000000000/gram trichoderma viride 5-
20%th, 200,000,000,000/gram bacillus subtilis 5-20%, 10,000,000,000/gram bacillus laterosporus 1-10%, 5,000,000,000/gram jelly
Sample bacillus 5-20%, 100,000,000,000/gram bacillus megaterium 0.5-5%, 5,000,000,000/gram mycorrhizal fungi 0.1-5%, 50
Hundred million/gram photosynthetic bacteria 1-10%, 5,000,000,000/gram Paecilomyces lilacinus 5-20% and glucose surplus, each component summation 100%.
Further, the strain original powder includes the strain component of following mass ratio：1000000000/gram trichoderma viride
10%th, 200,000,000,000/gram bacillus subtilis 10%, 10,000,000,000/gram bacillus laterosporus 5%, 5,000,000,000/gram gel-shaped bud
Spore bacillus 10%, 100,000,000,000/gram bacillus megaterium 2%, 5,000,000,000/gram mycorrhizal fungi 2%, 5,000,000,000/gram photosynthetic bacteria
5%th, 5,000,000,000/gram Paecilomyces lilacinus 10% and glucose 46%.
The various droops that the complex composition of the biological control crop droop can be used in agricultural planting industry are occurred frequently
Crop, such as banana, dwarf banana.
A kind of method that complex composition using the biological control crop droop plants banana, including：(1) in perfume (or spice)
Any of several broadleaf plants seedling stage replaces Fertilizer application, mu 500kg-1000kg with above-mentioned complex composition；(2) with spade in each tree drip position
Put and dig four row application ditches by four direction, it is wide 20 centimeters, long 30 centimeters per about 20 centimeters of row application ditch depth；(3) banana seedling stage
1kg complex compositions are applied by each dressing furrow, every banana applies 4kg complex compositions；(4) the banana trophophase is applied as each
Fertile ditch applies 2kg complex compositions, and every banana applies 8kg complex compositions；(5) earthing after ditch spread should be carried out when using, is prevented
Only filled after direct sunlight (6) use using clear water leaching and play effect on overburden soil to activate culture propagation.
Beneficial effect of the present invention：
The viable count of a variety of compound bacterias in inventive formulation reached 239.5 hundred million/gram, they mutually promote, are mutually complementary
Fill, anti-soil-borne disease effect is far longer than single culture.Profitable strain mutually cooperates with, collective effect, can not only reach crop
Disease-resistant, diseases prevention, the effect of upgrading high yield, moreover it is possible to which, to improved soil, immeasurable effect is played in enhancement of environment.
Wherein, trichoderma viride is important to having the function that in plant pathology biological control.Its mechanism of action have with
Under it is several：(1) antibiotic is produced：When density of the trichoderma viride in soil reaches certain limit, to ensure oneself existence ring
Border, it will secrete some to other harmful microorganisms and material that the existence without the small insects of shell is inhibited, thus suppresses
Or reduce and make pathogen in object or without the small insects of shell, even become extinct, protect root system to be corroded from pest type；(2) heavy responsibilities of government
Raw effect：Hyperparasitism refers to identification to pathogen, contact, winds, penetrates and a series of complexity of parasitic consecutive steps
Process.During Trichoderma and pathogen interaction, host mycelia, which secretes some materials, makes Trichoderma tend to the life of host fungi
It is long, once host is identified by Trichoderma parasitic animal and plant, parasitism will be established.After Trichoderma identifies to host fungi, trichoderma
Mycelia grows along host mycelia Parallel Growth and spiral wound, and produces appresorium shape branch and be adsorbed on host mycelia, leads to
Secretion ectoenzyme dissolving cell membrane is crossed, host mycelia is penetrated, draws nutrition, and then pathogen is killed, this is Trichoderma conduct
The most important mechanism of Antagonistic Fungi；(3) bacteriolysis：Trichoderma viride can produce a variety of enzyme systems with bioactivity, such as：Fiber
Plain enzyme, chitinase, zytase etc., these enzymes, which can dissolve, kills some fungal diseases.Trichoderma viride is produced fiber
One of plain enzymatic activity highest bacterial strain；(4) Competition：Trichoderma encloses and can mushroomed out in rhizosphere, the leaf of plant, seizes plant
The site of body surface, a protective cover is formed, just as putting on boots to plant, prevent disease fungus from touching plant roots
System and blade surface, protect the plant root, leaf portion to be infected from above-mentioned pathogen with this, and ensure that plant can be healthily
Growth.
Bacillus subtilis can increase crop anti-adversity, fixed nitrogen, promote plant growth.Albumen in soil and organic matter,
Fat, carbohydrate are just easy to crop and absorb, utilize, supported so as to strengthen itself after microbial bacteria effect is degraded to cell cube molecule
Drag, root system is promoted to develop toward favourable aspect.Its cardinal principle is as follows：(1) antibiotic, the antibiotic of bacillus subtilis are produced
There are ribosomes and non-ribosomal to synthesize two classes, antibiotic can directly kill sickle-like bacteria；(2) bacteriocin is produced, bacteriocin is thin
The small molecular weight protein to other microorganisms with antibiosis of bacterium synthesis, has the effect of antibacterium and fungi；(3) produce
Raw chitinase and glucolase, both enzymes can destroy the cell membrane of sickle-like bacteria so that such fungi osmotic pressure is uneven
It is and dead.
Bacillus laterosporus energy promoting root growth, harmful bacteria and degraded heavy metal are killed, its microorganism species was metabolized in breeding, division
Cheng Zhongneng secretes substantial amounts of biochemical substances, such as biochemical enzymes, albumen, with improved soil it is biological, dissolve it chemically, promote
Crop root growth, and the propagation of pathogen can be suppressed.
Bacillusmusilaginosiengineering energy potassium decomposing, discharge micro in solvable phosphorus potassium element and calcium, sulphur, magnesium, iron, zinc, molybdenum, manganese etc.
Element, in order to crop absorption, utilize.With the biochemical action of biological bacteria, required for being discharged in itself from soil, decompositing plant
Various a great number of elements and trace element, strengthen its absorption and utilization to nutrients.
Bacillus megaterium energy phosphorus decomposing (phosphobacteria), there is the effect of organophosphor in degraded soil well.Utilize biology
Bacterium division, breeding when, secrete substantial amounts of biology enzyme, macromolecular complex in soil be converted into crop can absorb, utilize it is beneficial
Element and nutrient.
Mycorrhizal fungi can expand Root Absorption face, increase the absorption to the element (particularly phosphorus) outside primitive root hair absorption region
Ability.When the breeding of its bacterium, division, a large amount of gases of exhalation and mycelia, loosing soil can be made, and the biology enzyme secreted can stimulate children
Bud growth, germination, and root circle flora is established, the health of seedling is protected, with inner improved soil, beneficial to root system beneficial growth.
Photosynthetic bacteria can utilize hydrogen sulfide, carbon dioxide, water etc. to synthesize carbohydrate, amino acids, vitamins, nitrogen chemical combination
Thing, antiviral substance and physiological activator etc. are rich soils and promote the main force of animal and plant growth.
Paecilomyces lilacinus has preventing and treating efficiency to a variety of nematodes, is the preventing and treating most promising biological prevention and control agent of root-knot nematode.Its
It is disease-resistant, disease-prevention and insecticide principle is identical with above-mentioned trichoderma viride.It is intended to radical cure yellow leaf droop Zhiduo County and adds one of protecting valve.
The present invention carries strain original powder and is typically also referred to as function bacterium, and its bacterium can be given birth to for 0 degree in temperature with up to less than 80 degree
Deposit, humidity if being more than this humidity, can just be activated in a dormant state below 10%, make strain just start to grow using preceding
Consumed energy, it is not easy to maintain, and shorten composition with pathogen action time.Meet to supply it while above-mentioned condition
Enough, monomolecular organic matter, the source of nutrition being used as required for function bacterium, it will act on soil by dividing, breeding
A huge microbiologic population is formed in earth, in continuous breeding metabolism caused mycoprotein, biology enzyme and its
His metabolin is that crop absorbs and utilized well, meanwhile, the small pest type of no shell is run into, its albumen is can dissolve, is eliminated, reached
To the growth for reducing or suppressing atomic type insects in pathogen, soil, so as to increase the ability of crop disease-resistant diseases prevention, make crop
Root system is no longer destroyed, and required moisture, nutrient can continuously be absorbed from soil, so as to fundamentally prevent yellow leaf
The generation of droop.Nutrition carrier of the glucose as beneficial bacterium in the present invention, protects beneficial bacterium not to be activated at normal temperatures,
One of nutrient source as beneficial bacterium when activating, can also be as the nutriment that plant absorption utilizes.
The complex composition of the biological control crop droop of the present invention has that cost is low, without environmental pollution, has wide
Wealthy prospect, preventing and treating stability is good, is not likely to produce disease resistance, makes full use of the advantage of biological control, improve prevention effect, change
It has been apt to crop cultivation, the daily management of plantation, has reduced economic loss and cost accounting.
Embodiment
In order to better illustrate technical solution of the present invention problem to be solved, the technical scheme used and reach beneficial
Effect, it is expanded on further in conjunction with embodiment.What deserves to be explained is technical solution of the present invention is including but not limited to following
Embodiment.
Technical solution of the present invention and using effect are described further below in conjunction with instantiation.Employed in example
Strain is purchased from Guangdong Microbiological Culture Collection administrative center：Trichoderma viride (GIM3.141) bacillusmusilaginosiengineering
(GIM1.15), Bacillus Lateraporus (GIM1.404), Paecilomyces lilacinus (GIM3.405), bacillus megaterium (GIM1.270),
Used bacillus subtilis, photosynthetic bacteria and mycorrhizal fungi are the banana body and week that Hainan banana blight area survives
Collarette border extracts the strain harmless to the human body identified by Guangdong Province microorganism detection center.Various strains are routinely
Preparation method obtains, and makes production trichoderma viride living bacteria count >=109Cfu/g, bacillus subtilis living bacteria count >=2*
1012Cfu/g, bacillus laterosporus living bacteria count >=1010Cfu/g, bacillus megaterium living bacteria count >=1012Cfu/g glue
Freeze sample bacillus living bacteria count >=5*109Cfu/g, photosynthetic bacteria living bacteria count >=5*109Cfu/g, Paecilomyces lilacinus effect are lived
Bacterium number >=5*109Cfu/g, mycorrhizal fungi living bacteria count >=5*109Cfu/g, organic fertilizer are that the Leizhou City basis ecological agriculture is limited
Company produces.
The trichoderma viride of embodiment 1 and bacillus subtilis are combined to droop prevention effect
100 parts of organic fertilizers are coordinated to be mixed and made into composition A agent with 1 part of trichoderma viride respectively by quality；With 1 part of withered grass bud
Spore bacillus coordinates 100 parts of organic fertilizers to be mixed and made into composition B agent；With 0.5 part of trichoderma viride, 0.5 part of bacillus subtilis is matched somebody with somebody
Close 100 parts of organic fertilizers and be mixed and made into composition C agent.
Four pieces of soil properties of selection are consistent, fertility is uniformly medium, and the banana region that droop morbidity is heavier, are grouped into experiment
Field A, B, C and control group, and the banana planted to four pieces of ground is handled as follows respectively：
Experimental plot A (1 mu)：A composition A agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot B (1 mu)：A composition B agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot C (1 mu)：A composition C agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Compare field (1 mu)：Organic fertilizer is used alone as control in seedling stage and trophophase；
Each experimental plot uses ten point samplings, and every samples 10 plants, and droop is investigated, records and counts the incidence of disease
And control rate, computational methods are as follows：
The incidence of disease (%)=morbidity strain number/investigation total strain number × 100
Control rate (%)=(the control field incidence of disease-experimental plot incidence of disease)/control field incidence of disease × 100
Result of the test：The biological composition of the present invention can effectively reduce dead seedling it can be seen from the result of the test of table 1
Rate, there is obvious prevention effect to banana blight, wherein composition C agent is to banana blight control rate to more than 85%.Say
Bright trichoderma viride and bacillus subtilis are used cooperatively best to the prevention effect of banana blight.
The trichoderma viride of table 1 and bacillus subtilis combine the prevention effect to droop
| Group | Investigate strain number | Droop incidence of disease % | Control rate % |
| Experimental plot 1 | 100 | 20 | 63.6 |
| Experimental plot 2 | 100 | 15 | 72.7 |
| Experimental plot 3 | 100 | 8 | 85.4 |
| Compare field | 100 | 55 | -- |
The trichoderma viride of embodiment 2 and bacillus subtilis different proportion formula are to droop using effect
100 parts of organic fertilizers are coordinated to be mixed and made into combination with 0.7 part of trichoderma viride, 0.3 part of bacillus subtilis by quality
Thing A agent, 100 parts of organic fertilizers are coordinated to be mixed and made into composition B agent with 0.3 part of trichoderma viride, 0.7 part of bacillus subtilis, with
0.5 part of trichoderma viride, 0.5 part of bacillus subtilis coordinate 100 parts of organic fertilizers to be mixed and made into composition C agent,
Four pieces of soil properties of selection are consistent, fertility is uniformly medium, and the banana region that droop morbidity is heavier, packet
For experimental plot A, B, C and control group, and the banana planted to four pieces of ground is handled as follows respectively：
Experimental plot A (1 mu)：A composition A agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot B (1 mu)：A composition B agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot C (1 mu)：A composition C agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Compare field (1 mu)：Independent organic fertilizer is used in seedling stage and trophophase as control；
Each experimental plot uses ten point samplings, and every samples 10 plants, and droop is investigated, records and counts the incidence of disease
And control rate, computational methods are as follows：
The incidence of disease (%)=morbidity strain number/investigation total strain number × 100
Control rate (%)=(the control field incidence of disease-experimental plot incidence of disease)/control field incidence of disease × 100
Result of the test：Trichoderma viride and bacillus subtilis are used cooperatively to perfume (or spice) it can be seen from the result of the test of table 2
The prevention effect of any of several broadleaf plants droop has good result, wherein with trichoderma viride and bacillus subtilis in mass ratio 1:1 coordinates
When organic fertilizer using effect reach optimal.
The prevention effect of the trichoderma viride of table 2 and bacillus subtilis different proportion formula to droop
| Group | Investigate strain number | Droop incidence of disease % | Control rate % |
| Experimental plot 1 | 100 | 14 | 74.5 |
| Experimental plot 2 | 100 | 10 | 81.8 |
| Experimental plot 3 | 100 | 8 | 85.4 |
| Compare field | 100 | 55 | -- |
The trichoderma viride of embodiment 3 and bacillus subtilis coordinate the organic fertilizer of different proportion to droop using effect
The core of radical cure yellow leaf droop is that function bacterium, but function bacterium plays a role and also be unable to do without organic fertilizer, the two
Complement each other.Microorganism in nature is " ubiquitous, always to have ", either air, river, ground, a few kms
Deep deep-sea, animals and plants body are divided into beneficial microbe and harmful microorganism with the presence of a large amount of microorganisms." active probiotic "
That is beneficial microbe, the jinx of the various disease existence of plant, and the jinx of various pollutions are not only.Its existence is except sky
Gas and water is graded natural endowment, it is necessary to is relied on certain organic matter and is depended on for existence and breed for nutrient source, and solid-state is organic
Fertilizer, Water soluble fertilizer etc. are stood in the breach the source in the nutritional benefit source for becoming function bacterium.Prevented based on the root growth to crop, disease pest
Control, the research of soil improvement action, certain proportion relation between discovery feature bacterium and organic fertilizer be present, in this proportionate relationship internal strength
The viable bacteria of energy bacterium can be only achieved the optimum state of the common profit of alternate with organic fertilizer.
500 parts of organic fertilizers are coordinated to be mixed and made into combination with 0.5 part of trichoderma viride, 0.5 part of bacillus subtilis by quality
Thing A agent, 200 parts of organic fertilizers are coordinated to be mixed and made into composition B agent with 0.5 part of trichoderma viride, 0.5 part of bacillus subtilis, with
0.5 part of trichoderma viride, 0.5 part of bacillus subtilis coordinate 100 parts of organic fertilizers to be mixed and made into composition C agent, with 0.5 part of green
Trichoderma, 0.5 part of bacillus subtilis coordinate 50 parts of organic fertilizers to be mixed and made into composition D agent, with 0.5 part of trichoderma viride, 0.5
Part bacillus subtilis coordinates 20 parts of organic fertilizers to be mixed and made into composition E agent.
Six pieces of soil properties of selection are consistent, fertility is uniformly medium, and the banana region that droop morbidity is heavier, packet
For experimental plot A, B, C, D, E and control group, and the banana planted to six pieces of ground is handled as follows respectively：
Experimental plot A (1 mu)：A composition A agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot B (1 mu)：A composition B agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot C (1 mu)：A composition C agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot D (1 mu)：A composition D agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot E (1 mu)：A composition E agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Compare field (1 mu)：Independent organic fertilizer is used in seedling stage and trophophase as control；
Each experimental plot uses ten point samplings, and every samples 10 plants, and droop is investigated, records and counts the incidence of disease
And control rate, computational methods are as follows：
The incidence of disease (%)=morbidity strain number/investigation total strain number × 100
Control rate (%)=(the control field incidence of disease-experimental plot incidence of disease)/control field incidence of disease × 100
Result of the test：Trichoderma viride and bacillus subtilis coordinate organic fertilizer to make it can be seen from the result of the test of table 3
It is better to prevent and treat the effect of droops for more its of trichoderma viride usage amount in the range of certain usage amount, wherein bacterium powder and has
Machine fertilizer ratio is 1:100 and 1:50 effects reach optimal.
The trichoderma viride of table 3 and bacillus subtilis coordinate the organic fertilizer of different proportion to the prevention effect of droop
| Group | Investigate strain number | Droop incidence of disease % | Control rate % |
| Experimental plot A (500:1) | 100 | 18 | 67.3 |
| Experimental plot B (200:1) | 100 | 14 | 74.5 |
| Experimental plot C (100:1) | 100 | 8 | 85.4 |
| Experimental plot D (50:1) | 100 | 8 | 85.4 |
| Experimental plot E (20:1) | 100 | 9 | 83.6 |
| Compare field | 100 | 55 | -- |
The strain cooperation that embodiment 4 increases different proportion on equal portions trichoderma viride and bacillus subtilis proportioning is organic
Fertilizer is to droop using effect.
Composition A agent is prepared：By the strain component of following mass ratio：1000000000/gram 5 parts of trichoderma viride, 200,000,000,000/gram
5 parts of bacillus subtilis, 100,000,000,000/gram 1 part of bacillus laterosporus, 5,000,000,000/gram 20 parts of bacillusmusilaginosiengineering, 1000
Hundred million/gram 0.5 part of bacillus megaterium, 5,000,000,000/gram 0.1 part of mycorrhizal fungi, 5,000,000,000/gram 10 parts of photosynthetic bacteria, 5,000,000,000/gram
It is made after the 53.4 parts of mixing of 5 parts of Paecilomyces lilacinus and glucose.Microbial inoculum and organic fertilizer are pressed 1:Combination is made after the mixing of 100 ratios
Thing A agent.
Composition B agent is prepared：By the strain component of following mass ratio：1000000000/gram 10 parts of trichoderma viride, 200,000,000,000/
Gram 10 parts of bacillus subtilis, 10,000,000,000/gram 5 parts of bacillus laterosporus, 5,000,000,000/gram 10 parts of bacillusmusilaginosiengineering,
100000000000/gram 2 parts of bacillus megaterium, 5,000,000,000/gram 2 parts of mycorrhizal fungi, 5,000,000,000/gram 5 parts of photosynthetic bacteria, 5,000,000,000/gram
It is made after the 46 parts of mixing of 10 parts of Paecilomyces lilacinus and glucose.Microbial inoculum and organic fertilizer are pressed 1:Combination is made after the mixing of 100 ratios
Thing B agent.
Composition C agent is prepared：By the strain component of following mass ratio：1000000000/gram 20 parts of trichoderma viride, 200,000,000,000/
Gram 20 parts of bacillus subtilis, 10,000,000,000/gram 10 parts of bacillus laterosporus, 5,000,000,000/gram 5 parts of bacillusmusilaginosiengineering,
100000000000/gram 5 parts of bacillus megaterium, 5,000,000,000/gram 5 parts of mycorrhizal fungi, 5,000,000,000/gram 1 part of photosynthetic bacteria, 5,000,000,000/gram
It is made after the 14 parts of mixing of 20 parts of Paecilomyces lilacinus and glucose.Microbial inoculum and organic fertilizer are pressed 1:Combination is made after the mixing of 100 ratios
Thing C agent.
Composition D agent is prepared：By the strain component of following mass ratio：1000000000/gram 10 parts of trichoderma viride, 200,000,000,000/
Gram the 80 parts of mixing of 10 parts of bacillus subtilis and glucose after be made.Microbial inoculum and organic fertilizer are pressed 1:Made after the mixing of 100 ratios
Obtain composition D agent.
Five pieces of soil properties of selection are consistent, fertility is uniformly medium, and the banana region that droop morbidity is heavier, packet
For experimental plot A, B, C, D and control group, and the banana planted to four pieces of ground is handled as follows respectively：
Experimental plot A (1 mu)：A composition A agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot B (1 mu)：A composition B agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot C (1 mu)：A composition C agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Experimental plot D (1 mu)：A composition D agent is used respectively in seedling stage and trophophase, every banana of seedling stage uses 4kg,
Every banana of trophophase uses 8kg.
Compare field (1 mu)：Independent organic fertilizer is used in seedling stage and trophophase as control；
Each experimental plot uses ten point samplings, and every samples 10 plants, and droop is investigated, records and counts the incidence of disease
And control rate, computational methods are as follows：
The incidence of disease (%)=morbidity strain number/investigation total strain number × 100
Control rate (%)=(the control field incidence of disease-experimental plot incidence of disease)/control field incidence of disease × 100
Result of the test：Trichoderma viride and bacillus subtilis coordinate certain proportion it can be seen from the result of the test of table 4
Other microbial bacterias it is better to the effect for preventing and treating droop than only using trichoderma viride and bacillus subtilis, illustrate other auxiliary
Bacterium has gain effect to trichoderma viride and bacillus subtilis, and wherein composition B proportioning effect reaches optimal.
The strain that table 4 increases different proportion on equal portions trichoderma viride and bacillus subtilis proportioning coordinates organic fertilizer pair
Droop using effect.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
1. a kind of complex composition of biological control crop droop, it is characterised in that including organic fertilizer and strain original powder；It is described
The weight ratio of organic fertilizer and strain original powder is 100:1-20：1.
2. composition according to claim 1, it is characterised in that the weight ratio of organic fertilizer and strain the original powder is 100:
1。
3. composition according to claim 1, it is characterised in that the organic fertilizer moisture content is less than 10%, organic fertilizer institute
The organic matter contained reaches more than 45%, humic acid more than 8%.
4. composition according to claim 1, it is characterised in that the strain original powder includes trichoderma viride and withered grass bud
Spore bacillus；Trichoderma viride and the mass ratio of bacillus subtilis are 7:3-3:7.
5. composition according to claim 4, it is characterised in that the quality of the trichoderma viride and bacillus subtilis
Than for 1:1.
6. composition according to claim 1, it is characterised in that the strain original powder includes the strain group of following mass ratio
Point：1000000000/gram trichoderma viride 5-20%, 200,000,000,000/gram bacillus subtilis 5-20%, 10,000,000,000/gram side spore gemma
Bacillus 1-10%, 5,000,000,000/gram bacillusmusilaginosiengineering 5-20%, 100,000,000,000/gram bacillus megaterium 0.5-5%, 5,000,000,000/
Gram mycorrhizal fungi 0.1-5%, 5,000,000,000/gram photosynthetic bacteria 1-10%, 5,000,000,000/gram Paecilomyces lilacinus 5-20% and glucose more than
Amount, each component summation 100%.
7. composition according to claim 6, it is characterised in that the strain original powder includes the strain group of following mass ratio
Point：1000000000/gram trichoderma viride 10%, 200,000,000,000/gram bacillus subtilis 10%, 10,000,000,000/gram bacillus laterosporus
5%th, 5,000,000,000/gram bacillusmusilaginosiengineering 10%, 100,000,000,000/gram bacillus megaterium 2%, 5,000,000,000/gram mycorrhizal fungi
2%th, 5,000,000,000/gram photosynthetic bacteria 5%, 5,000,000,000/gram Paecilomyces lilacinus 10% and glucose 46%.
8. the complex composition of the biological control crop droop described in claim any one of 1-7 is applied to banana or dwarf banana
In plantation.
9. the method that usage right requires the complex composition plantation banana of any one of the 1-7 biological control crop droop,
It is characterised in that it includes following steps：(1) used in banana seedling stage and state complex composition and replace Fertilizer application, mu 500kg-
1000kg；(2) four row application ditches are dug by four direction in the tree line position that drips with spade, per 20 centimeters of row application ditch depth, wide by 20
Centimetre, 30 centimeters of length；(3) banana seedling stage applies 1kg complex compositions by each dressing furrow, and every banana applies 4kg compounding groups
Compound；(4) the banana trophophase applies 2kg complex compositions by each dressing furrow, and every banana applies 8kg complex compositions；(5)
Earthing after ditch spread should be carried out during use, prevents direct sunlight；(6) fill numerous to activate strain on overburden soil using clear water leaching after use
Grow performance effect.
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| CN109485498A (en) * | 2018-11-29 | 2019-03-19 | 长沙浩博生物技术有限公司 | A kind of method of preparation and use of high organic complex microorganism flushing and spreading fertilizer |
| CN109497089A (en) * | 2019-01-22 | 2019-03-22 | 山东土木启生物科技有限公司 | With the complex micro organism fungicide and preparation method for reducing continuous cropping, wilt disease |
| CN111004061A (en) * | 2019-11-30 | 2020-04-14 | 广西金穗农业集团有限公司 | Anti-wilt agent applied to banana fertilizer and preparation method thereof |
| CN111206002A (en) * | 2020-02-19 | 2020-05-29 | 郑州职业技术学院 | Compound bacterium for resisting rehmannia wilt and application thereof |
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| CN109497089A (en) * | 2019-01-22 | 2019-03-22 | 山东土木启生物科技有限公司 | With the complex micro organism fungicide and preparation method for reducing continuous cropping, wilt disease |
| CN111004061A (en) * | 2019-11-30 | 2020-04-14 | 广西金穗农业集团有限公司 | Anti-wilt agent applied to banana fertilizer and preparation method thereof |
| CN111206002A (en) * | 2020-02-19 | 2020-05-29 | 郑州职业技术学院 | Compound bacterium for resisting rehmannia wilt and application thereof |
| CN111849490A (en) * | 2020-07-21 | 2020-10-30 | 成都华宏生物科技有限公司 | Composition for biologically preventing and treating pepper phytophthora blight and preparation method and application thereof |
| CN111849490B (en) * | 2020-07-21 | 2022-01-25 | 成都华宏生物科技有限公司 | Composition for biologically preventing and treating pepper phytophthora blight and preparation method and application thereof |
| CN112772679A (en) * | 2021-02-01 | 2021-05-11 | 广西南亚热带农业科学研究所 | Biopesticide for sugarcane fungal diseases and application thereof |
| CN112772679B (en) * | 2021-02-01 | 2021-12-17 | 广西南亚热带农业科学研究所 | Biopesticide for sugarcane fungal diseases and application thereof |
| CN115226455A (en) * | 2022-07-22 | 2022-10-25 | 海南宝绿春农业开发有限公司 | Fertilizing method for conditioning banana continuous cropping obstacle soil |
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