CN111004061A - Anti-wilt agent applied to banana fertilizer and preparation method thereof - Google Patents
Anti-wilt agent applied to banana fertilizer and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A—HUMAN NECESSITIES
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Abstract
The invention discloses an anti-wilt agent applied to banana fertilizer and a preparation method thereof, belonging to the field of agricultural chemistry. The anti-wilt agent comprises the following components in parts by weight: animal viscera, animal bone meal, rhizoma Coptidis, oil cake, mixed microbial inoculum, ear skin, dimethyl sulfoxide, MgSO4Allyl propyl disulfide, xanthan gum. The preparation method of the anti-wilt agent is characterized by comprising the following steps: s1: mixing animal viscera, animal bone powder, rhizoma Coptidis, oil cake, wheat ear skin, dimethyl sulfoxide, and MgSO4And the allyl propyl disulfide is uniformly mixed, high-frequency resonance grinding and crushing are carried out, the mixed powder is treated by ozone plasma, xanthan gum and deionized water are added, sealing and stirring are carried out, then the mixed microbial inoculum is added, and the anti-withering agent is obtained after stirring and uniform mixing. The anti-wilt agent can be organically combined with banana fertilizer, and can improve the capacity of preventing wilt while providing nutrients for bananas.
Description
Technical Field
The invention relates to the field of agricultural chemistry, in particular to an anti-wilt agent applied to banana fertilizer and a preparation method thereof.
Background
Bananas in the broad sense include both the banana and plantain species, both of the family Musaceae, the genus Musa, and are the second largest fruit crop in the world, with yields second only to citrus. Bananas are perennial evergreen large herbs, monocotyledons, and have huge biomass. The main cultivar of banana in China is Musa AAA group, Dwarf Cavendish subgroup, namely banana, and the plant height is generally 2-4 m. Banana plants consist of roots, bulbs, pseudostems, leaves, fruit stalks, fruit ears, and the like. The base of the plant is called corm (also called root corm), and the main stalk formed by overlapping leaf sheaths is called pseudostem (also called pseudostem). The corm is commonly called banana head, is an organ for growing root systems, leaves and absorbing buds, and is also a central part for supplying and storing nutrients.
The banana Panama wilt disease spreads quickly, and once the banana Panama wilt disease occurs, the banana wilt disease is difficult to eradicate completely, thereby seriously affecting the development of the banana industry of each banana producing country in the world. At present, many scholars screen chemical agents aiming at banana wilt, and researchers pay attention to biological control and do many related biological control experiments to prove that some bacteria have good antagonistic action on fusarium wilt. Although there have been some results in chemical and biological control of banana wilt, the effect is not obvious.
Disclosure of Invention
The invention aims to provide an anti-wilt agent applied to banana fertilizer and a preparation method thereof, and aims to solve the technical problem that the anti-wilt agent in the prior art cannot be combined with the fertilizer and applied.
For this purpose, the invention proposes the following solutions:
an anti-wilt agent applied to banana fertilizer comprises the following components in parts by weight: 34-42 parts of animal viscera, 25-33 parts of animal bone meal, 18-30 parts of rhizoma coptidis from Sichuan of China, 20-26 parts of oil cake, 12-20 parts of mixed microbial inoculum, 8-15 parts of wheat ear skin, 3-6 parts of dimethyl sulfoxide and MgSO42-6 parts of allyl propyl disulfide, 0.9-3.5 parts of xanthan gum and 0.7-2.6 parts of xanthan gum.
Preferably, the anti-wilt agent comprises the following components in parts by weight: 39 parts of animal viscera, 30 parts of animal bone meal, 26 parts of rhizoma coptidis from Sichuan of China, 22 parts of oil cake, 17 parts of mixed microbial inoculum, 11 parts of wheat ear skin, 4 parts of dimethyl sulfoxide and MgSO44 parts of allyl propyl disulfide, 1.9 parts of xanthan gum and 1.6 parts of xanthan gum.
Preferably, the mixed microbial inoculum is prepared by mixing trichoderma harzianum, bacillus licheniformis, bacillus thuringiensis and candida utilis according to the mass ratio of 2-4:1-1.5:0.4-0.7: 1-2.
Preferably, the mixed microbial inoculum is prepared by mixing trichoderma harzianum, bacillus licheniformis, bacillus thuringiensis and candida utilis according to the mass ratio of 2.7:1.3:0.5: 1.4.
Preferably, the content of strains in the trichoderma harzianum, bacillus licheniformis, bacillus thuringiensis and candida utilis in the mixed microbial inoculum is 2-5 hundred million cfu/g.
A preparation method of an anti-wilt agent applied to banana fertilizer comprises the following steps:
s1: mixing animal viscera, animal bone powder, rhizoma Coptidis, oil cake, wheat ear skin, dimethyl sulfoxide, and MgSO4Mixing the allyl propyl disulfide uniformly, and grinding the mixture by high-frequency resonance grinding with the vibration frequency of 43.1-49.2Hz to obtain mixed powder A;
s2: treating the mixed powder A with ozone plasma, adding 18-55 parts of xanthan gum and deionized water, and hermetically stirring at 34-40 deg.C for 25-40min to obtain mixed powder B;
s3: and adding the mixed microbial inoculum into the mixed powder B, stirring for 40-65min, and uniformly mixing to obtain the anti-wilt agent.
Preferably, in the step S1, the high frequency resonance milling is performed to 400-600 μm.
Preferably, in step S1, the vibration frequency is 46.8 Hz.
Preferably, in the step S2, 39 parts of deionized water is added.
Preferably, in the step S2, the mixture is sealed and stirred for 33min at the temperature of 36 ℃.
Compared with the prior art, the invention has the advantages that:
1. as can be seen from Table 1, the incidence of blight in examples was controlled to 5.7% or less, and the individual plant yields were all 29.7kg or more, whereas example 1 was the most preferable example. The average of the blight morbidity of examples 1 to 3 was 4.80%, which was 67.07% lower than that of comparative examples 1 to 4; the average number of fusarium wilt deaths of examples 1-3 was 87.23% lower than that of comparative examples 1-4; the average yield of the single plants in examples 1 to 3 is 31.2kg, which is 15.68% higher than that of the single plants in comparative examples 1 to 4; it can be seen that the lack of a certain component in the anti-wilt agent can greatly affect the disease prevention effect and the planting effect.
2. In the anti-wilt agent, the mixed microbial inoculum can generate substances such as organic acid, hormone and the like which are beneficial to absorption and utilization of plants, effectively inhibit pathogenic bacteria such as fusarium oxysporum and the like, effectively reduce the record of infection of young tissues with wilt, and improve the leaf area of bananas, thereby promoting good growth of plants; dimethyl sulfoxide is an important aprotic polar solvent which is soluble in both water and an organic solvent, can change the permeability of a biological membrane to electrolytes, medicaments, poisons and metabolites, is beneficial to the penetration of an anti-withering agent to plants, and improves the absorption rate of the plants to the nutrients and the anti-withering agent; the sol molecules of xanthan gum can form super-bonded banded spiral copolymer to form a fragile gel-like network structure, so that a strong emulsification stabilizing effect is shown, the stability of the anti-wilt agent in different temperature and pH value environments is further enhanced, and the stability of the anti-wilt agent in fertilizer preparation is improved. By comprehensively comparing the data in the table 1, the synergistic effect can be achieved by using the mixed microbial inoculum, the dimethyl sulfoxide and the xanthan gum, and the growth of banana trees can be promoted while the anti-blight capability of bananas is improved.
3. The anti-wilt agent disclosed by the invention is slightly influenced by environmental factors when being used, takes effect quickly, not only can timely disinfect diseased plants on the spot, but also can greatly shorten the withering time of the diseased plants, is convenient for transferring and destroying diseased plant residues, effectively avoids the diffusion of pathogenic bacteria in a plantation, is strong in stability, can be added into a banana fertilizer, can be used while fertilizing, can play a role in preventing and treating the wilt disease for a long time, is small in environmental pollution when being used, and has no adverse effect on other plants.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments. It should be emphasized that the following description is merely exemplary in nature and is not intended to limit the scope of the invention or its application.
Example 1
An anti-wilt agent applied to banana fertilizer comprises the following components in parts by weight: 39 parts of animal viscera, 30 parts of animal bone meal, 26 parts of rhizoma coptidis from Sichuan of China, 22 parts of oil cake, 17 parts of mixed microbial inoculum, 11 parts of wheat ear skin, 4 parts of dimethyl sulfoxide and MgSO44 parts of allyl propyl disulfide, 1.9 parts of xanthan gum and 1.6 parts of xanthan gum.
The mixed microbial inoculum is prepared by mixing trichoderma harzianum, bacillus licheniformis, bacillus thuringiensis and candida utilis according to the mass ratio of 2.7:1.3:0.5:1.4, and the content of each strain is 4 hundred million cfu/g.
A preparation method of an anti-wilt agent applied to banana fertilizer comprises the following steps:
s1: mixing animal viscera, animal bone powder, rhizoma Coptidis, oil cake, wheat ear skin, dimethyl sulfoxide, and MgSO4Mixing allyl propyl disulfide uniformly, and grinding to 550 μm by high-frequency resonance at vibration frequency of 46.8Hz to obtain mixed powder A;
s2: treating the mixed powder A with ozone plasma, adding 39 parts of xanthan gum and deionized water, and hermetically stirring at 36 ℃ for 33min to obtain mixed powder B;
s3: and adding the mixed microbial inoculum into the mixed powder B, stirring for 52min, and uniformly mixing to obtain the anti-wilt agent.
The fertilizer capable of preventing and treating banana wilt, which is applied in the invention, comprises the following components in parts by weight: n38 parts, P2O531 parts of, K217 parts of O, 25 parts of wormcast, 12 parts of nitrohumic acid, 11 parts of composite microbial inoculum, 15 parts of plant ash, 14 parts of anti-wilt agent, 5 parts of groundsel, 4 parts of European water chestnut, 3 parts of cellulose hydroxyethyl ether, 1.6 parts of 2-chloro-6-trichloromethylpyridine, 0.8 part of cellulose gum, 0.17 part of boron and 0.11 part of molybdenum.
The complex microbial inoculum comprises the following components in parts by weight: 38 parts of lactobacillus plantarum, 28 parts of EM microbial inoculum, 20 parts of putrefying spirillum and 16 parts of phanerochaete chrysosporium, wherein the content of each strain is 8 hundred million cfu/g.
The N element in the fertilizer component is provided by urea and monoammonium phosphate, and P2O5Provided by calcium magnesium phosphate fertilizer and monoammonium phosphate, K2O is provided by potassium chloride.
The preparation method of the fertilizer capable of preventing and treating banana vascular wilt comprises the following steps:
s1: n, P will be mixed2O5And K2Grinding, then sieving with a 70-mesh sieve, and continuously grinding residues;
s2: n, P will be mixed2O5、K2Putting O, wormcast, nitrohumic acid, plant ash, groundsel, European water chestnut, boron and molybdenum into a fermentation tank, adding 330 parts of water, stirring and mixing, stacking the fermentation pile to 3m, keeping the water content of the fermentation product to be 34%, covering the fermentation pile with a heat-preservation coating, turning the fermentation pile when the temperature of the fermentation product reaches 52 ℃, and turning the fermentation pile once every 12 hours until the pile is turned for 4;
s3: removing the heat-preservation coating on the fermentation pile after the step S2, naturally cooling to 26 ℃, adding water into the composite microbial inoculum for diluting to 3% of mass concentration, making a round hole with the diameter of 5cm at the top of the fermentation pile every 50cm, directly introducing the round hole to the middle of the fermentation pile, and then pouring the diluted composite microbial inoculum; turning over every 4 hours, adding an anti-withering agent, cellulose hydroxyethyl ether, 2-chloro-6-trichloromethylpyridine and cellulose gum during the first turning over, turning over for 4 times until the total fermentation time is 20 days, and deeply turning over the fermented substance every 8 days after the previous turning over to obtain the fertilizer.
The fertilizer application method used in the invention comprises the following steps: digging up old roots of bananas in soil needing new planting by a plough, deeply ploughing the soil for 0.52m, simultaneously spreading 67 kg of lime per mu, mixing a phoxim missible oil diluent and a carbendazim wettable powder under a natural condition for 6 days, spraying the mixture on the surface of the soil, planting after 16 days, planting new banana saplings, and paving and burying peat soil during the cultivation of the new banana saplings;
and mixing 690 times diluted phoxim missible oil solution and carbendazim wettable powder according to the mass ratio of 1:1.2, and spraying 53kg of the phoxim missible oil solution and carbendazim wettable powder on the surface of soil per mu.
And (3) performing hole application by adopting fertilizers, digging 3 holes at a position 46cm away from the root of the banana when applying the base fertilizer, wherein the specification of the holes is 56cm multiplied by 45cm, applying 53kg of the base fertilizer per mu averagely, covering a layer of straw, covering soil, and pushing up a soil pile with the height of 16cm at the top of the fertilizing hole after covering the soil.
When in topdressing, 3 holes are dug at the position 40cm away from the root of the banana, the specification of the holes is 45cm multiplied by 45 multiplied by 28cm, a layer of straw is used for covering after fertilization, and then soil is covered; dividing the banana fruit period into a flower bud differentiation period, a bud extraction period and a fruit strengthening period, and applying the fertilizer once per month when applying the fertilizer in holes, wherein the total fertilizer input amount in the flower bud differentiation period is 326 g/plant; the total fertilizer input amount in the bud drawing period is 441 g/plant; the total fertilizer input amount in the fruit strengthening period is 153g per plant.
Example 2
An anti-wilt agent applied to banana fertilizer comprises the following components in parts by weight: 42 parts of animal viscera, 33 parts of animal bone meal, 30 parts of rhizoma coptidis from Sichuan of China, 26 parts of oil cake, 20 parts of mixed microbial inoculum, 15 parts of wheat ear skin, 6 parts of dimethyl sulfoxide and MgSO46 parts of allyl propyl disulfide, 3.5 parts of xanthan gum and 2.6 parts of xanthan gum.
The mixed microbial inoculum is prepared by mixing trichoderma harzianum, bacillus licheniformis, bacillus thuringiensis and candida utilis according to the mass ratio of 4:1.5:0.7:2, and the content of each strain is 5 hundred million cfu/g.
A preparation method of an anti-wilt agent applied to banana fertilizer comprises the following steps:
s1: mixing animal viscera, animal bone powder, rhizoma Coptidis, oil cake, wheat ear skin, dimethyl sulfoxide, and MgSO4Mixing allyl propyl disulfide uniformly, and grinding to 600 μm by high-frequency resonance to obtain mixed powder A with vibration frequency of 49.2 Hz;
s2: treating the mixed powder A with ozone plasma, adding 55 parts of xanthan gum and deionized water, and hermetically stirring at 40 ℃ for 40min to obtain mixed powder B;
s3: and adding the mixed microbial inoculum into the mixed powder B, stirring for 65min, and uniformly mixing to obtain the anti-wilt agent.
The fertilizer capable of preventing and treating banana wilt, which is applied in the invention, comprises the following components in parts by weight: n38 parts, P2O531 parts of, K217 parts of O, 25 parts of wormcast, 12 parts of nitrohumic acid, 11 parts of composite microbial inoculum, 15 parts of plant ash, 14 parts of anti-wilt agent, 5 parts of groundsel, 4 parts of European water chestnut, 3 parts of cellulose hydroxyethyl ether, 1.6 parts of 2-chloro-6-trichloromethylpyridine, 0.8 part of cellulose gum, 0.17 part of boron and 0.11 part of molybdenum.
The complex microbial inoculum comprises the following components in parts by weight: 38 parts of lactobacillus plantarum, 28 parts of EM microbial inoculum, 20 parts of putrefying spirillum and 16 parts of phanerochaete chrysosporium, wherein the content of each strain is 8 hundred million cfu/g.
The N element in the fertilizer component is provided by urea and monoammonium phosphate, and P2O5Provided by calcium magnesium phosphate fertilizer and monoammonium phosphate, K2O is provided by potassium chloride.
The preparation method of the fertilizer capable of preventing and treating banana vascular wilt comprises the following steps:
s1: n, P will be mixed2O5And K2Grinding, then sieving with a 70-mesh sieve, and continuously grinding residues;
s2: n, P will be mixed2O5、K2O, wormcast and nitroPutting humic acid, plant ash, groundsel, European water chestnut, boron and molybdenum into a fermentation tank, adding 330 parts of water, stirring and mixing, stacking the fermentation pile to 3m, keeping the water content of the fermentation product between 34%, covering the fermentation pile with a heat-preservation coating, turning the fermentation product when the temperature of the fermentation product reaches 52 ℃, and turning the fermentation pile once every 12 hours until the fermentation pile is turned for 4 hours;
s3: removing the heat-preservation coating on the fermentation pile after the step S2, naturally cooling to 26 ℃, adding water into the composite microbial inoculum for diluting to 3% of mass concentration, making a round hole with the diameter of 5cm at the top of the fermentation pile every 50cm, directly introducing the round hole to the middle of the fermentation pile, and then pouring the diluted composite microbial inoculum; turning over every 4 hours, adding an anti-withering agent, cellulose hydroxyethyl ether, 2-chloro-6-trichloromethylpyridine and cellulose gum during the first turning over, turning over for 4 times until the total fermentation time is 20 days, and deeply turning over the fermented substance every 8 days after the previous turning over to obtain the fertilizer.
The fertilizer application method used in the invention comprises the following steps: digging up old roots of bananas in soil needing new planting by a plough, deeply ploughing the soil for 0.52m, simultaneously spreading 67 kg of lime per mu, mixing a phoxim missible oil diluent and a carbendazim wettable powder under a natural condition for 6 days, spraying the mixture on the surface of the soil, planting after 16 days, planting new banana saplings, and paving and burying peat soil during the cultivation of the new banana saplings;
and mixing 690 times diluted phoxim missible oil solution and carbendazim wettable powder according to the mass ratio of 1:1.2, and spraying 53kg of the phoxim missible oil solution and carbendazim wettable powder on the surface of soil per mu.
And (3) performing hole application by adopting fertilizers, digging 3 holes at a position 46cm away from the root of the banana when applying the base fertilizer, wherein the specification of the holes is 56cm multiplied by 45cm, applying 53kg of the base fertilizer per mu averagely, covering a layer of straw, covering soil, and pushing up a soil pile with the height of 16cm at the top of the fertilizing hole after covering the soil.
When in topdressing, 3 holes are dug at the position 40cm away from the root of the banana, the specification of the holes is 45cm multiplied by 45 multiplied by 28cm, a layer of straw is used for covering after fertilization, and then soil is covered; dividing the banana fruit period into a flower bud differentiation period, a bud extraction period and a fruit strengthening period, and applying the fertilizer once per month when applying the fertilizer in holes, wherein the total fertilizer input amount in the flower bud differentiation period is 326 g/plant; the total fertilizer input amount in the bud drawing period is 441 g/plant; the total fertilizer input amount in the fruit strengthening period is 153g per plant.
Example 3
An anti-wilt agent applied to banana fertilizer comprises the following components in parts by weight: 34 parts of animal viscera, 25 parts of animal bone meal, 18 parts of rhizoma coptidis from Sichuan of China, 20 parts of oil cake, 12 parts of mixed microbial inoculum, 8 parts of wheat ear skin, 3 parts of dimethyl sulfoxide and MgSO42 parts of allyl propyl disulfide, 0.9 part of xanthan gum and 0.7 part of xanthan gum.
The mixed microbial inoculum is prepared by mixing trichoderma harzianum, bacillus licheniformis, bacillus thuringiensis and candida utilis according to the mass ratio of 2:1:0.4:1, and the content of each strain is 2 hundred million cfu/g.
A preparation method of an anti-wilt agent applied to banana fertilizer comprises the following steps:
s1: mixing animal viscera, animal bone powder, rhizoma Coptidis, oil cake, wheat ear skin, dimethyl sulfoxide, and MgSO4The allyl propyl disulfide is evenly mixed and ground to 400-600 mu m by high-frequency resonance and the vibration frequency is 43.1Hz to obtain mixed powder A;
s2: treating the mixed powder A with ozone plasma, adding 18 parts of xanthan gum and deionized water, and hermetically stirring at 34 ℃ for 25min to obtain mixed powder B;
s3: and adding the mixed microbial inoculum into the mixed powder B, stirring for 40min, and uniformly mixing to obtain the anti-wilt agent.
The fertilizer capable of preventing and treating banana wilt, which is applied in the invention, comprises the following components in parts by weight: n38 parts, P2O531 parts of, K217 parts of O, 25 parts of wormcast, 12 parts of nitrohumic acid, 11 parts of composite microbial inoculum, 15 parts of plant ash, 14 parts of anti-wilt agent, 5 parts of groundsel, 4 parts of European water chestnut, 3 parts of cellulose hydroxyethyl ether, 1.6 parts of 2-chloro-6-trichloromethylpyridine, 0.8 part of cellulose gum, 0.17 part of boron and 0.11 part of molybdenum.
The complex microbial inoculum comprises the following components in parts by weight: 38 parts of lactobacillus plantarum, 28 parts of EM microbial inoculum, 20 parts of putrefying spirillum and 16 parts of phanerochaete chrysosporium, wherein the content of each strain is 8 hundred million cfu/g.
The N element in the fertilizer component is provided by urea and monoammonium phosphate, and P2O5Provided by calcium magnesium phosphate fertilizer and monoammonium phosphate, K2O is provided by potassium chloride.
The preparation method of the fertilizer capable of preventing and treating banana vascular wilt comprises the following steps:
s1: n, P will be mixed2O5And K2Grinding, then sieving with a 70-mesh sieve, and continuously grinding residues;
s2: n, P will be mixed2O5、K2Putting O, wormcast, nitrohumic acid, plant ash, groundsel, European water chestnut, boron and molybdenum into a fermentation tank, adding 330 parts of water, stirring and mixing, stacking the fermentation pile to 3m, keeping the water content of the fermentation product to be 34%, covering the fermentation pile with a heat-preservation coating, turning the fermentation pile when the temperature of the fermentation product reaches 52 ℃, and turning the fermentation pile once every 12 hours until the pile is turned for 4;
s3: removing the heat-preservation coating on the fermentation pile after the step S2, naturally cooling to 26 ℃, adding water into the composite microbial inoculum for diluting to 3% of mass concentration, making a round hole with the diameter of 5cm at the top of the fermentation pile every 50cm, directly introducing the round hole to the middle of the fermentation pile, and then pouring the diluted composite microbial inoculum; turning over every 4 hours, adding an anti-withering agent, cellulose hydroxyethyl ether, 2-chloro-6-trichloromethylpyridine and cellulose gum during the first turning over, turning over for 4 times until the total fermentation time is 20 days, and deeply turning over the fermented substance every 8 days after the previous turning over to obtain the fertilizer.
The fertilizer application method used in the invention comprises the following steps: digging up old roots of bananas in soil needing new planting by a plough, deeply ploughing the soil for 0.52m, simultaneously spreading 67 kg of lime per mu, mixing a phoxim missible oil diluent and a carbendazim wettable powder under a natural condition for 6 days, spraying the mixture on the surface of the soil, planting after 16 days, planting new banana saplings, and paving and burying peat soil during the cultivation of the new banana saplings;
and mixing 690 times diluted phoxim missible oil solution and carbendazim wettable powder according to the mass ratio of 1:1.2, and spraying 53kg of the phoxim missible oil solution and carbendazim wettable powder on the surface of soil per mu.
And (3) performing hole application by adopting fertilizers, digging 3 holes at a position 46cm away from the root of the banana when applying the base fertilizer, wherein the specification of the holes is 56cm multiplied by 45cm, applying 53kg of the base fertilizer per mu averagely, covering a layer of straw, covering soil, and pushing up a soil pile with the height of 16cm at the top of the fertilizing hole after covering the soil.
When in topdressing, 3 holes are dug at the position 40cm away from the root of the banana, the specification of the holes is 45cm multiplied by 45 multiplied by 28cm, a layer of straw is used for covering after fertilization, and then soil is covered; dividing the banana fruit period into a flower bud differentiation period, a bud extraction period and a fruit strengthening period, and applying the fertilizer once per month when applying the fertilizer in holes, wherein the total fertilizer input amount in the flower bud differentiation period is 326 g/plant; the total fertilizer input amount in the bud drawing period is 441 g/plant; the total fertilizer input amount in the fruit strengthening period is 153g per plant.
Comparative example 1
The anti-wilt agent and the preparation method, the fertilizer preparation and application method and the example 1 are basically the same, except that the anti-wilt agent is not added with mixed bacteria agent.
Comparative example 2
The anti-wilt agent and method of preparation, fertilizer preparation and application methods were essentially the same as in example 1, except that no dimethyl sulfoxide was added to the anti-wilt agent.
Comparative example 3
The anti-wilt agent and method of preparation, fertilizer preparation and application methods were essentially identical to those of example 1, except that no xanthan gum was added to the anti-wilt agent.
Comparative example 4
The anti-wilt agent and the preparation method, the fertilizer preparation and application method and the example 1 are basically the same, except that the anti-wilt agent is not added with mixed bacteria agent, allyl propyl disulfide and xanthan gum.
In the general part of the golden ear agriculture group in Longan county, Guangxi, 7 test areas with the area of 5 mu are divided, 145 banana seedlings with the plant height of 14-20cm are planted in each mu by the methods of examples 1-3 and comparative examples 1-4 respectively, the yield of bananas in the harvest period, the number of fusarium wilt infections and the number of fusarium wilt deaths of each group are counted, and the fusarium wilt infection rate, the number of fusarium wilt deaths and the average single banana yield of each group are calculated and specifically shown in Table 1.
TABLE 1 wilt infection, mortality and Banana yield Table
As can be seen from Table 1, the incidence of blight in examples was controlled to 5.7% or less, and the individual plant yields were all 29.7kg or more, whereas example 1 was the most preferable example. The average of the blight morbidity of examples 1 to 3 was 4.80%, which was 67.07% lower than that of comparative examples 1 to 4; the average number of fusarium wilt deaths of examples 1-3 was 87.23% lower than that of comparative examples 1-4; the average yield of the single plants in examples 1 to 3 is 31.2kg, which is 15.68% higher than that of the single plants in comparative examples 1 to 4; it can be seen that the lack of a certain component in the anti-wilt agent can greatly affect the disease prevention effect and the planting effect.
In the anti-wilt agent, the mixed microbial inoculum can generate substances such as organic acid, hormone and the like which are beneficial to absorption and utilization of plants, effectively inhibit pathogenic bacteria such as fusarium oxysporum and the like, effectively reduce the record of infection of young tissues with wilt, and improve the leaf area of bananas, thereby promoting good growth of plants; dimethyl sulfoxide is an important aprotic polar solvent which is soluble in both water and an organic solvent, can change the permeability of a biological membrane to electrolytes, medicaments, poisons and metabolites, is beneficial to the penetration of an anti-withering agent to plants, and improves the absorption rate of the plants to the nutrients and the anti-withering agent; the sol molecules of xanthan gum can form super-bonded banded spiral copolymer to form a fragile gel-like network structure, so that a strong emulsification stabilizing effect is shown, the stability of the anti-wilt agent in different temperature and pH value environments is further enhanced, and the stability of the anti-wilt agent in fertilizer preparation is improved. By comprehensively comparing the data in the table 1, the synergistic effect can be achieved by using the mixed microbial inoculum, the dimethyl sulfoxide and the xanthan gum, and the growth of banana trees can be promoted while the anti-blight capability of bananas is improved.
The foregoing is a more detailed description of the invention in connection with specific/preferred embodiments and is not intended to limit the practice of the invention to those descriptions. It will be apparent to those skilled in the art that various substitutions and modifications can be made to the described embodiments without departing from the spirit of the invention, and such substitutions and modifications are to be considered as within the scope of the invention.
Claims (10)
1. An anti-wilt agent applied to banana fertilizer is characterized by comprising the following components in parts by weight: 34-42 parts of animal viscera, 25-33 parts of animal bone meal, 18-30 parts of rhizoma coptidis from Sichuan of China, 20-26 parts of oil cake, 12-20 parts of mixed microbial inoculum, 8-15 parts of wheat ear skin, 3-6 parts of dimethyl sulfoxide and MgSO42-6 parts of allyl propyl disulfide, 0.9-3.5 parts of xanthan gum and 0.7-2.6 parts of xanthan gum.
2. The anti-wilting agent for banana fertilizer according to claim 1, wherein said anti-wilting agent comprises the following components in parts by weight: 39 parts of animal viscera, 30 parts of animal bone meal, 26 parts of rhizoma coptidis from Sichuan of China, 22 parts of oil cake, 17 parts of mixed microbial inoculum, 11 parts of wheat ear skin, 4 parts of dimethyl sulfoxide and MgSO44 parts of allyl propyl disulfide, 1.9 parts of xanthan gum and 1.6 parts of xanthan gum.
3. The anti-wilt agent for banana fertilizer as defined in claim 1, wherein said mixed bacterial agent is prepared by mixing Trichoderma harzianum, Bacillus licheniformis, Bacillus thuringiensis and Candida utilis in a mass ratio of 2-4:1-1.5:0.4-0.7: 1-2.
4. The anti-wilt agent for banana fertilizer as claimed in claim 3, wherein the mixed bacterial agent is prepared by mixing Trichoderma harzianum, Bacillus licheniformis, Bacillus thuringiensis and Candida utilis in a mass ratio of 2.7:1.3:0.5: 1.4.
5. The anti-wilt agent for banana fertilizer as set forth in claim 3, wherein the content of the bacterial species in Trichoderma harzianum, Bacillus licheniformis, Bacillus thuringiensis and Candida utilis in the mixed bacterial agent is 2-5 hundred million cfu/g.
6. A method for preparing an anti-wilt agent for banana fertilizer according to any of claims 1-5, comprising the following steps:
s1: mixing animal viscera, animal bone powder, rhizoma Coptidis, oil cake, wheat ear skin, dimethyl sulfoxide, and MgSO4Mixing the allyl propyl disulfide uniformly, and grinding the mixture by high-frequency resonance grinding with the vibration frequency of 43.1-49.2Hz to obtain mixed powder A;
s2: treating the mixed powder A with ozone plasma, adding 18-55 parts of xanthan gum and deionized water, and hermetically stirring at 34-40 deg.C for 25-40min to obtain mixed powder B;
s3: and adding the mixed microbial inoculum into the mixed powder B, stirring for 40-65min, and uniformly mixing to obtain the anti-wilt agent.
7. The method as set forth in claim 6, wherein the step S1 comprises grinding to 400-600 μm by HF resonance.
8. The method of claim 6, wherein in step S1, the vibration frequency is 46.8 Hz.
9. The method of claim 6, wherein 39 parts of deionized water is added in step S2.
10. The method of claim 6, wherein the step S2 is performed by hermetically stirring at 36 deg.C for 33 min.
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