CN112772679B

CN112772679B - Biopesticide for sugarcane fungal diseases and application thereof

Info

Publication number: CN112772679B
Application number: CN202110132734.5A
Authority: CN
Inventors: 施泽升; 唐利球; 彭崇; 郭强; 梁永检; 闭德金; 秦昌鲜; 马文清; 罗晟昇; 何洪良; 韦海球; 廖韦卫; 江清梅
Original assignee: Guangxi South Subtropical Agricultural Science Research Institute
Current assignee: Guangxi South Subtropical Agricultural Science Research Institute
Priority date: 2021-02-01
Filing date: 2021-02-01
Publication date: 2021-12-17
Anticipated expiration: 2041-02-01
Also published as: CN112772679A

Abstract

The invention belongs to the field of pesticides, and particularly relates to a biological pesticide for sugarcane fungal diseases and application thereof. A biopesticide for sugarcane fungal diseases, said biopesticide comprising Bacillus subtilis, Trichoderma, betaine; the bacillus subtilis is 100 hundred million/g bacillus subtilis wettable powder, and the trichoderma is 2 hundred million/g trichoderma water dispersible granules; also comprises a foliar fertilizer; the foliar fertilizer contains N, P, K or one or more of trace elements. The biological pesticide provided by the invention is used for preventing and treating sugarcane fungal diseases, and is preferably used for preventing and treating sugarcane brown spot germs.

Description

Biopesticide for sugarcane fungal diseases and application thereof

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a biological pesticide for sugarcane fungal diseases and application thereof.

Background

Sugarcane is the most important sugar crop in China, and the planting area and the sugar yield of the sugarcane account for more than 90 percent of the whole country. In recent years, with the development of the cane sugar industry, the disease problem generated in the sugarcane planting process is increasingly prominent, and the sugarcane disease becomes a main factor influencing the sugarcane yield and restricting the stable development of the cane sugar industry. According to investigation, the sugarcane diseases known in the world are as many as 160, and more than 60 sugarcane diseases are found in China, wherein about 50 sugarcane diseases are found in a continental sugarcane area. These diseases not only affect the yield and sugar content of sugarcane, but also severely impair the ratoon of sugarcane and cause the degeneration of varieties.

At present, diseases of domestic sugarcane main production areas mainly comprise three types of fungal diseases, bacterial diseases, virus diseases and mycoplasmal diseases. Therefore, the research on the sugarcane diseases is particularly important.

Disclosure of Invention

The invention relates to a biological pesticide for sugarcane fungal diseases and application thereof.

The specific technical scheme provided by the invention is as follows:

a biopesticide for sugarcane fungal diseases, said biopesticide comprising Bacillus subtilis, Trichoderma, betaine; the bacillus subtilis is 100 hundred million/g bacillus subtilis wettable powder, and the trichoderma is 2 hundred million/g trichoderma water dispersible granules.

Preferably, the mass ratio of the biological pesticide of 100 hundred million/g bacillus subtilis wettable powder to 2 hundred million/g trichoderma water dispersible granules is 10:1-1: 10.

Preferably, the mass ratio of the total content of 100 hundred million/g bacillus subtilis wettable powder and 2 hundred million/g trichoderma water dispersible granules to the betaine of the biological pesticide is 10:1-1: 5.

Preferably, the biological pesticide also comprises foliar fertilizer; the foliar fertilizer contains N, P, K or one or more of trace elements.

Preferably, the biological pesticide is used for preventing and treating sugarcane fungal diseases.

Preferably, the sugarcane fungal disease is sugarcane brown spot pathogen.

Compared with the prior art, the invention has the following beneficial effects:

1. after the betaine is combined with the bacillus subtilis and the trichoderma, the medicament has obvious inhibition effect on the hypha growth of the brown spot pathogen of the sugarcane; particularly, when the mass ratio of the mixture 1+ the betaine is 5:1, the EC50 value of the mixture on the brown spot pathogen of sugarcane is 356.70 mg/L; the betaine has the effect of enhancing the sterilization effect of the mixed preparation of the bacillus subtilis and the trichoderma.

2. The betaine can obviously improve the control effect of the bacillus subtilis and the trichoderma on the brown spot germs of the sugarcane.

Detailed Description

The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is illustrative only and should not be taken as limiting the invention as described in detail in the claims

Example 1: screening of sugarcane brown spot pathogen prevention and treatment medicament

Test strains: the brown spot pathogen of sugarcane is obtained by sampling and separating in a sugarcane production test base by adopting a tissue separation method, is stored in a PDA slant culture medium after being separated and purified, and is stored at low temperature (4 ℃). The PDA culture medium formula comprises: 200g of peeled potatoes, 20g of cane sugar, 20g of agar and 1000mL of water.

Reagent to be tested: 100 hundred million/g bacillus subtilis wettable powder (Jingbo agrichemical science and technology Co., Ltd.) and 2 hundred million/g trichoderma water dispersible granules (Ciona Arisaema, Inc.); the test agents and the concentrations used are shown in Table 1.

TABLE 1 test Agents and use concentrations

The determination method comprises the following steps: a hypha growth rate determination method is adopted, and fungus cakes with the diameter of 5mm are taken from the colony of the purified and propagated sugarcane brown spot pathogen by a puncher and are inoculated to the centers of flat plates of a culture medium containing medicine and a blank control culture medium (1 fungus cake is inoculated in each dish) and are respectively cultured in a constant temperature box at the temperature of 28 ℃. And 4d, respectively measuring the diameters of the colonies of the brown spot germs of the sugarcane by adopting a cross method, and calculating the inhibition rates of different treatments on the growth of hyphae.

And (3) data analysis: and carrying out data statistical analysis by adopting DPS software, and carrying out linear regression by taking the logarithmic value of the concentration of the bactericide as x and the corresponding value of the hypha growth inhibition rate as y to obtain a virulence regression equation and the virulence EC50 value of the medicament on the target bacteria.

Measurement results and analysis thereof

TABLE 2 determination of toxicity of test agents against Saccharum officinarum

Note: "-" indicates no inhibition.

As can be seen from Table 2, the EC50 value of 100 hundred million/g Bacillus subtilis and 2 hundred million/g Trichoderma (mixture 1) to Saccharum officinarum L.var.canna is 1159.39mg/L after being mixed according to the mass ratio of 1: 1; when the concentration of the betaine is 10000mg/L, the growth of hypha of the brown spot pathogen of the sugarcane is not inhibited. After the betaine is combined with bacillus subtilis and trichoderma for use, the medicament has obvious inhibition effect on the hypha growth of the brown spot pathogen of sugarcane; particularly, when the mass ratio of the mixture 1+ the betaine is 5:1, the EC50 value of the mixture on the brown spot pathogen of sugarcane is 356.70 mg/L; namely, the betaine has the effect of enhancing the sterilization effect of the mixed agent of the bacillus subtilis and the trichoderma.

Example 2: test of field drug effect

And (3) experimental design: area of 45m per cell²Repeat 3 times, use random block arrangement. The 1 st pesticide is applied when the sugarcane brown spot disease happens sporadically, the 2 nd pesticide is applied at intervals of 15d, the pesticides are uniformly applied to the front and back surfaces of sugarcane plant leaves, the dropping of fog drops on the leaf surfaces is taken as the standard, and other pesticides are not used in the test period. Disease onset was investigated and recorded 14d after the 2 nd dose. Sampling was done at random 5 points per cell, 2 plants were taken at each point, and 10 leaves were investigated per plant. Classification standard of sugarcane brown spot:

no diseases exist on the 0-level whole stem and leaf, and the plant grows healthily;

the leaf below the level 1 whole plant 1/4 is diseased with a small amount of disease symptoms, and each leaf has 1-25 disease spots (spots) or the disease spots occupy less than 25% of the leaf area;

the leaf with the disease of less than 2-level whole plant 1/4-1/2 has obvious disease symptoms, 26-50 disease spots (spots) are formed on each leaf or the area of the disease spots (spots) is 25-50 percent;

the leaf of 3-grade whole plant 1/2-3/4 has disease, obvious disease symptom, 51-75 disease spots on each leaf or 50.1-75% of disease spots in leaf area;

the disease of the 4-level whole plant 3/4 and above leaves has obvious disease symptoms, and each leaf has more than 75 disease spots or large disease spots or the disease spots account for more than 75 percent of the leaf area;

the disease spots of the 5-grade whole plant leaves are enlarged and connected into large disease spots, so that the leaves are dry and even the plants die.

The disease index ═ Σ (number of diseased leaves at each stage × representative value at each stage)/(total number of investigated leaves × highest representative value at each stage) × 100;

control effect (%) - (control disease index-treatment disease index)/control disease index × 100.

TABLE 3 field control of the test agents against Saccharum officinarum

Note: the dosage of the active ingredients is the dosage of the mixture 1.

As can be seen from Table 2, the field control effect of the compound 1 on the sugarcane brown spot is 75.4% when the dosage is 150 g/mu; when the dosage of the mixture 1+ betaine (mass ratio is 5:1) is 150 g/mu, the field control effect on the sugarcane brown spot is 92.8%; the improvement is 17.4%.

In addition, as can be seen from table 2, betaine has no biological activity against alternaria alternata, and it can be seen that betaine can significantly improve the control effect of bacillus subtilis and trichoderma on alternaria alternata.

The foregoing is only a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that modifications may be made without departing from the invention as claimed in the invention and these modifications are within the scope of the invention.

Claims

1. Use of a biopesticide for controlling sugarcane brown spot, wherein the biopesticide comprises bacillus subtilis, trichoderma, and betaine; the bacillus subtilis is 100 hundred million/g bacillus subtilis wettable powder, and the trichoderma is 2 hundred million/g trichoderma water dispersible granules; the mass ratio of 100 hundred million/g bacillus subtilis wettable powder to 2 hundred million/g trichoderma water dispersible granules is 10:1-1: 10; the mass ratio of the total content of 100 hundred million/g bacillus subtilis wettable powder and 2 hundred million/g trichoderma water dispersible granules to the betaine is 10:1-1: 5.

2. The use according to claim 1, characterized in that the biopesticide also comprises a foliar fertilizer; the foliar fertilizer contains N, P, K or one or more of trace elements.