CN105638748A - Trichoderma harzianum and pythium oligadrum compounded microbial agent and application thereof - Google Patents
Trichoderma harzianum and pythium oligadrum compounded microbial agent and application thereof Download PDFInfo
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Abstract
The invention discloses a trichoderma harzianum and pythium oligadrum compounded microbial agent. The microbial agent comprises trichoderma harzianum, pythium oligadrum and a pesticide carrier. The composition is compounded from trichoderma harzianum and pythium oligadrum and can increase the crop yield, improve the soil and improve the crop quality; besides, the microbial agent has a better control effect on pathogenic bacteria of soil-borne diseases, has a remarkable synergistic interaction effect and can control seedling death, root rot, green wilting, dead standing, yellow wilt, butt rot, stalk rot, damping off and other common agricultural diseases.
Description
Technical field
The present invention relates to a kind of composite antimicrobial composition, the microbial inoculum composite particularly to a kind of Trichoderma harzianum and pythium oligandrum and application thereof.
Background technology
Trichoderma harzianum is the one in trichoderma, mycelia is very thin colourless, and tool separates, multi-branched, conidiophore bears from the side shoot of mycelia, to raw or alternate, sporogenic stigma doleiform estranged or taper, conidium mostly is spherical, sporoderm tool pustule, aeruginous, is white cotton fiber shape at the beginning of bacterium colony in PDA culture medium, is dirty-green afterwards. Trichoderma harzianum can be used to prevent the microbial plant diseases of cause of disease such as, sclerotinite mould by pythium spp, Rhizoctonia solani Kuhn, Fusarium spp., bread mold, post spore, Sclerotium rolfsii as a kind of biocontrol microorganisms. Trichoderma harzianum encloses can mushroom out at the rhizosphere of plant, leaf; seize the site on plant surface; form a protective cover; just as putting on boots to plant; pathogenic fungi is stoped to touch root system of plant and blade surface; protect plant root, leaf portion to infect from above-mentioned pathogen with this, and ensure that plant can blossom out.
Pythium oligandrum is the parasitical fungi that a kind of aggressivity existed in nature is very strong, can at multiple kinds of crops rhizosphere colonization, not only crop will not be produced pathogenic effects, and can also suppress or kill other pathomycete and soil-borne pathogen, induction plant produces defense response, reduces the invasion of pathogen; Meanwhile, pythium oligandrum produce secretions and various enzyme, be the good growth promoting activating agent of plant, can promote that crop root is grown, raising Nutrient Absorption. 20 various plants pathogenic fungi or other oomycetes are had parasitization by pythium oligandrum bacterium, and the process of parasitization includes absorption, is wound around and penetrates. Pythium oligandrum is invaded in the tissue of pathomycete or other oomycetes by mycelia, gradually uses up its internal nutrient, is finally reached killing action.
In the cultivation of crop, various soil-borne diseases such as dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, damping off etc. and usually to perplex farmer, solution is usually the special pesticide using various specific aim diseases, the excessive use of pesticide, not only makes soil compaction, and polluted underground water resource, cause the unsustainable development of agricultural, and disease pathogen Drug resistance strengthens, what have to increasing pesticide makes consumption, causes vicious cycle. Trichoderma harzianum and pythium oligandrum, for the preventing and treating of plant mycosis, have respective advantage, but, still not by composite to Trichoderma harzianum and the pythium oligandrum report for preventing and treating soil-borne disease.Additionally, current Trichoderma harzianum and pythium oligandrum single dose culture presevation time are short, cause that result of use declines.
Summary of the invention
It is an object of the invention to provide a kind of Trichoderma harzianum and the composite microbial inoculum of pythium oligandrum and application thereof, strengthen respective result of use, and extend the preservation time of strain.
A kind of Trichoderma harzianum and the composite microbial inoculum of pythium oligandrum, described microbial inoculum is by Trichoderma harzianum, pythium oligandrum and farm chemical carrier composition.
Described farm chemical carrier is emulsifying agent, excipient, synergist, protects microbial inoculum, dispersant, one or more in Plant fiber.
Described emulsifying agent is selected from diacetyl tartarate list double glyceride, glyceryl monostearate, benzene ethyl phenyl polyoxyethylene ether, fatty alcohol-polyoxyethylene ether, fatty alkyl glycine betaine, ethylene glycol, cocoanut fatty acid diethanolamide, one or more in monoglyceride.
Described excipient is selected from polyvinyl alcohol, chitin, maltodextrin, trehalose, carboxymethyl starch, sodium starch glycollate, poly-(Isosorbide-5-Nitrae-��-D-glucosan), lactose, mannitol, dextran, glycine, one or more in polylysine.
Described synergist is selected from molasses, Rare Earth Lanthanum, cerium, Fructus solani xanthocarpi, fulvic acid, tea saponin, cocamido propyl betaine, ethylenediaminetetraacetic acid zinc, ethylenediaminetetraacetic acid manganese, benzene oxycarboxylic acid, one or more in fluosilicate.
Described guarantor's microbial inoculum is selected from bacillus subtilis fungus extract, yeast extract, Eupatorium cannabinum L. (Eupatorium coelestinum L.) ash, rice hull ash, mugwort powder, Herba Artemisiae Annuae powder, polymyxin, zeolite, one or more in brown sugar.
Described dispersant is selected from 5,5-dimethyltrimethylene carbonate methyl ester, SYN glue, polyoxyethylene alkylphenol ether, polyacrylamide, BTA, phosphoric acid five sodium, guar gum, poly-aspartate, polyglutamic acid, n-amyl alcohol, one or more in Siloxane-Oxyalkylene Copolymers.
Described Plant fiber is selected from coconut fibre, cotton fiber, bombax cotton, sisal fiber, abaca fibre, flax fiber, hemp, one or more in tossa.
Above-mentioned Trichoderma harzianum and the application in controlling plant diseases of the pythium oligandrum composite microbial inoculum.
Described plant disease is dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow withers, root-rot, brown foot rot, one or more in dampinging off.
Compared with prior art, there is advantages that the compositions of the present invention adopts Trichoderma harzianum and pythium oligandrum composite, crop yield can be increased, improvement soil, improve crop quality, the pathogen of soil-borne disease is had good prevention effect simultaneously, has obvious cooperative synergism effect, it is possible to the preventing and treating dead Seedling of crop, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, the agricultural Common Diseases such as damping off.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail, it is to be understood that protection scope of the present invention is not by the restriction of detailed description of the invention.
The reagent that following example adopt is:
Trichoderma harzianum (Trichodermaharzianum) preparation, containing trichoderma harzianum 1X108Individual/g preparation.
Pythium oligandrum (PythiumOligandrum) preparation, containing trichoderma harzianum 1X108Individual/g preparation.
Use content:
(1) Trichoderma harzianum preparation 0.5Kg/ mu;
(2) Trichoderma harzianum preparation 1.5Kg/ mu;
(3) pythium oligandrum preparation 0.5Kg/ mu;
(4) pythium oligandrum preparation 1.5Kg/ mu;
(5) Trichoderma harzianum preparation 0.5Kg/ mu+pythium oligandrum preparation 0.5Kg/ mu;
(6) Trichoderma harzianum preparation 0.5Kg/ mu+pythium oligandrum preparation 1.5Kg/ mu;
(7) Trichoderma harzianum preparation 1.5Kg/ mu+pythium oligandrum preparation 0.5Kg/ mu;
(8) Trichoderma harzianum preparation 1.5Kg/ mu+pythium oligandrum preparation 1.5Kg/ mu;
(9)CK
Being Wuzhishan Area, Haikou City, Hainan Province for examination soil, soil moisture content (water: dry ground) is 29.90%, pH6.97, soil organic matter content 36.6g/kg, NH4+-N3.6mg/kg, NO3--N56.4mg/kg, available phosphorus 534.0mg/kg, available potassium 1029.0mg/kg, belong to sandy loam.
Embodiment 1 corn yield increasing is tested
Before corn planting, being ploughed deeply in soil, uniformly apply the preparation of above-mentioned content, the nitrogenous fertilizer of same weight is used in comparison (CK), during results, calculates corn yield increasing rate;
Corn yield increasing rate %:100X (processes per mu yield-comparison per mu yield)/comparison per mu yield;
Calculate rate of growth according to Colby method (1966) formula and the rate of growth with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical potentiation of mixture; N is the quantity of mixed medicament; X1Represent the rate of growth after using the 1st kind of medicament; X2Represent the rate of growth after using the 2nd kind of pharmaceutical agent; XnRepresent the rate of growth after using n medicament. When the actual rate of growth of mixture is more than theoretical rate of growth, represent potentiation; When the actual rate of growth of mixture is less than theoretical rate of growth, represent antagonism. Measurement result is as shown in table 1:
The mixed effect of increasing production to Semen Maydis of table 1 Trichoderma harzianum preparation+pythium oligandrum
As it can be seen from table 1 Trichoderma harzianum preparation yield to Semen Maydis mixed with the four of pythium oligandrum kinds of different amounts all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: diacetyl tartarate list double glyceride 0.05g/g preparation; Maltodextrin 0.03g/g preparation; Fructus solani xanthocarpi 0.03g/g preparation; Rice hull ash 0.01g/g preparation; 5,5-dimethyltrimethylene carbonate methyl ester 0.03g/g preparation; Bombax cotton 0.01g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1 year, and the retention time of pythium oligandrum preparation is 1 year.
Embodiment 2 resists dead seedling diseases to test
Choosing the experimental plot that the dead seedling diseases of Fructus Capsici often occurs, before pepper planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Capsici length, to 8-9 sheet leaf, calculates the dead seedling diseases incidence rate of Fructus Capsici;
Fructus Capsici dead seedling diseases suppression ratio %:100X (check plot Fructus Capsici dead seedling diseases diseased plant number-treatment region Fructus Capsici dead seedling diseases diseased plant number) the dead seedling diseases diseased plant number of/check plot Fructus Capsici;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 2:
The mixed prevention effect to the dead seedling diseases of Fructus Capsici of table 2 Trichoderma harzianum preparation+pythium oligandrum
From table 2 it can be seen that the suppression to Fructus Capsici dead seedling diseases mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, synergism is obvious.Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: benzene ethyl phenyl polyoxyethylene ether 0.01g/g preparation; Trehalose 0.21g/g preparation; Rare Earth Lanthanum 0.01g/g preparation; Yeast extract 0.08g/g preparation; Polyoxyethylene alkylphenol ether 0.01g/g preparation; Sisal fiber 0.24g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1.5 years, and the retention time of pythium oligandrum preparation is 1 year.
Embodiment 3 resistance to bacterial wilt is tested
Choosing the experimental plot that Fructus Momordicae charantiae bacterial wilt often occurs, before Fructus Momordicae charantiae is planted, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Momordicae charantiae length, to 6-9 sheet leaf, calculates Fructus Momordicae charantiae bacterial wilt incidence rate;
Fructus Momordicae charantiae bacterial wilt suppression ratio %:100X (check plot Fructus Momordicae charantiae bacterial wilt diseased plant number-treatment region Fructus Momordicae charantiae bacterial wilt diseased plant number)/check plot Fructus Momordicae charantiae bacterial wilt diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 3:
The mixed prevention effect to Fructus Momordicae charantiae bacterial wilt of table 3 Trichoderma harzianum preparation+pythium oligandrum
From table 3 it can be seen that the suppression to Fructus Momordicae charantiae bacterial wilt mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: glyceryl monostearate 0.05g/g preparation; Chitin 0.20g/g preparation; Fulvic acid 0.01g/g preparation; Eupatorium cannabinum L. (Eupatorium coelestinum L.) ash 0.08g/g preparation; Polyacrylamide 0.01g/g preparation; Coconut fibre 0.22g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1 year, and the retention time of pythium oligandrum preparation is 1.5 years.
The anti-blight experiment of embodiment 4
Choosing the experimental plot that Semen Pisi sativi blight often occurs, before Semen Pisi sativi is planted, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Semen Pisi sativi length, to 7-10 sheet leaf, calculates Semen Pisi sativi blight incidence rate;
Semen Pisi sativi blight suppression ratio %:100X (check plot Semen Pisi sativi blight diseased plant number-treatment region Semen Pisi sativi blight diseased plant number)/check plot Semen Pisi sativi blight diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 4:
The mixed prevention effect to Semen Pisi sativi blight of table 4 Trichoderma harzianum preparation+pythium oligandrum
From table 4, it can be seen that the suppression to Semen Pisi sativi blight mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: fatty alkyl glycine betaine 0.01g/g preparation;Carboxymethyl starch 0.11g/g preparation; Cerium 0.01g/g preparation; Mugwort powder 0.11g/g preparation; Eupatorium cannabinum L. (Eupatorium coelestinum L.) ash 0.08g/g preparation; Phosphoric acid five sodium 0.01g/g preparation; Flax fiber 0.22g/g preparation. Described mugwort powder is that Radix Artemisia ordosicae dries the powder being ground into. Described Eupatorium cannabinum L. (Eupatorium coelestinum L.) ash is the ashes that the rhizome of Eupatorium cannabinum L. (Eupatorium coelestinum L.) burns till.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1 year, and the retention time of pythium oligandrum preparation is 1 year. If above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise the lazurite powder of 0.01g/g preparation, then the activity-maintaining time of Trichoderma harzianum preparation is 3 years, and the retention time of pythium oligandrum preparation is 4 years, it is suppressed that the effect of Semen Pisi sativi blight is constant.
The anti-root rot experiment of embodiment 5
Choosing the experimental plot that Fructus Luffae root rot often occurs, before Fructus Luffae is planted, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Luffae length, to 7-10 sheet leaf, calculates Fructus Luffae root rot incidence rate;
Fructus Luffae root rot suppression ratio %:100X (check plot Fructus Luffae root rot diseased plant number-treatment region Fructus Luffae root rot diseased plant number)/check plot Fructus Luffae root rot diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 5:
The mixed prevention effect to Fructus Luffae root rot of table 5 Trichoderma harzianum preparation+pythium oligandrum
As can be seen from Table 5, the suppression to Fructus Luffae root rot mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, and synergism is obvious. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: cocoanut fatty acid diethanolamide 0.01g/g preparation; Poly-(1,4-��-D-glucosan) 0.18g/g preparation; Tea saponin 0.01g/g preparation; Cocamido propyl betaine 0.04g/g preparation; Ethylenediaminetetraacetic acid zinc 0.04g/g preparation; SYN glue 0.04g/g preparation; Abaca fibre 0.22g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1.5 years, and the retention time of pythium oligandrum preparation is 1.5 years. If above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise the nanometer Montmorillonitum powder of 0.01g/g preparation, then the activity-maintaining time of Trichoderma harzianum preparation is 4 years, and the retention time of pythium oligandrum preparation is 4 years, it is suppressed that the effect of Semen Pisi sativi blight is constant.
The anti-base rot disease experiment of embodiment 6
Choosing the experimental plot that Fructus Lycopersici esculenti base rot disease often occurs, before tomato planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Lycopersici esculenti length, to 7-10 sheet leaf, calculates Fructus Lycopersici esculenti base rot disease incidence rate;
Fructus Lycopersici esculenti base rot disease suppression ratio %:100X (check plot Fructus Lycopersici esculenti base rot disease diseased plant number-treatment region Fructus Lycopersici esculenti base rot disease diseased plant number)/check plot Fructus Lycopersici esculenti base rot disease diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture.Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 6:
The mixed prevention effect to Fructus Lycopersici esculenti base rot disease of table 6 Trichoderma harzianum preparation+pythium oligandrum
As can be seen from Table 6, the suppression to Fructus Lycopersici esculenti base rot disease mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, and synergism is obvious. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: fatty alcohol-polyoxyethylene ether 0.01g/g preparation; Sodium starch glycollate 0.18g/g preparation; Dextran 0.01g/g preparation; Ethylenediaminetetraacetic acid manganese 0.04g/g preparation; Polymyxin 0.04g/g preparation; Poly-aspartate 0.04g/g preparation; Hemp 0.22g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1 year, and the retention time of pythium oligandrum preparation is 1 year. If above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise the medical stone powder of 0.01g/g preparation, then the activity-maintaining time of Trichoderma harzianum preparation is 3 years, and the retention time of pythium oligandrum preparation is 4 years, it is suppressed that the effect of Semen Pisi sativi blight is constant.
Embodiment 7 resisting damping-off is tested
Choosing the experimental plot that Fructus Lycopersici esculenti damping off often occurs, before tomato planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Lycopersici esculenti length, to 7-10 sheet leaf, calculates Fructus Lycopersici esculenti damping off incidence rate;
Fructus Lycopersici esculenti damping off suppression ratio %:100X (check plot kind Fructus Lycopersici esculenti damping off diseased plant number-treatment region Fructus Lycopersici esculenti damping off diseased plant number)/check plot Fructus Lycopersici esculenti damping off diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 7:
The mixed prevention effect to Fructus Lycopersici esculenti damping off of table 7 Trichoderma harzianum preparation+pythium oligandrum
As can be seen from Table 7, the suppression to Fructus Lycopersici esculenti damping off mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, and synergism is obvious. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following farm chemical carrier: monoglyceride 0.03g/g preparation; Polylysine 0.11g/g preparation; Zeolite 0.01g/g preparation; Brown sugar 0.04g/g preparation; N-amyl alcohol 0.01g/g preparation; Guar gum 0.04g/g preparation; Tossa 0.22g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1.5 years, and the retention time of pythium oligandrum preparation is 1 year.
Comparative example 1 corn yield increasing is tested
Before corn planting, being ploughed deeply in soil, uniformly apply the preparation of above-mentioned content, the nitrogenous fertilizer of same weight is used in comparison (CK), during results, calculates corn yield increasing rate;
Corn yield increasing rate %:100X (processes per mu yield-comparison per mu yield)/comparison per mu yield;
Calculate rate of growth according to Colby method (1966) formula and the rate of growth with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical potentiation of mixture; N is the quantity of mixed medicament; X1Represent the rate of growth after using the 1st kind of medicament; X2Represent the rate of growth after using the 2nd kind of pharmaceutical agent; XnRepresent the rate of growth after using n medicament. When the actual rate of growth of mixture is more than theoretical rate of growth, represent potentiation; When the actual rate of growth of mixture is less than theoretical rate of growth, represent antagonism. Measurement result is as shown in table 8:
The mixed effect of increasing production to Semen Maydis of table 8 Trichoderma harzianum preparation+pythium oligandrum
As can be seen from Table 8, Trichoderma harzianum preparation yield to Semen Maydis mixed with the four of pythium oligandrum kinds of different amounts does not have much changes, increases production inconspicuous. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following carrier: water 0.16g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1 month, and the retention time of pythium oligandrum preparation is 1 month.
Comparative example 2 resisting damping-off is tested
Choosing the experimental plot that Fructus Lycopersici esculenti damping off often occurs, before tomato planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Lycopersici esculenti length, to 7-10 sheet leaf, calculates Fructus Lycopersici esculenti damping off incidence rate;
Fructus Lycopersici esculenti damping off suppression ratio %:100X (check plot kind Fructus Lycopersici esculenti damping off diseased plant number-treatment region Fructus Lycopersici esculenti damping off diseased plant number)/check plot Fructus Lycopersici esculenti damping off diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 9:
The mixed prevention effect to Fructus Lycopersici esculenti damping off of table 9 Trichoderma harzianum preparation+pythium oligandrum
As can be seen from Table 9, the suppression to Fructus Lycopersici esculenti damping off mixed with the four of pythium oligandrum preparation kinds of different amounts of Trichoderma harzianum preparation shows certain effect, and synergism is inconspicuous. Above-mentioned Trichoderma harzianum preparation and pythium oligandrum preparation respectively comprise following carrier: water 0.46g/g preparation.
The activity-maintaining time of above-mentioned Trichoderma harzianum preparation is 1 month, and the retention time of pythium oligandrum preparation is 1 month.
The specific embodiments of the invention that are only disclosed above, but, the present invention is not limited to this, and the changes that any person skilled in the art can think of all should fall into protection scope of the present invention.
Claims (10)
1. a Trichoderma harzianum and the composite microbial inoculum of pythium oligandrum, it is characterised in that described microbial inoculum is by Trichoderma harzianum, pythium oligandrum and farm chemical carrier composition.
2. Trichoderma harzianum according to claim 1 and the composite microbial inoculum of pythium oligandrum, it is characterised in that described farm chemical carrier is emulsifying agent, excipient, synergist, protects microbial inoculum, dispersant, one or more in Plant fiber.
3. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of pythium oligandrum, it is characterized in that, described emulsifying agent is selected from diacetyl tartarate list double glyceride, glyceryl monostearate, benzene ethyl phenyl polyoxyethylene ether, fatty alcohol-polyoxyethylene ether, fatty alkyl glycine betaine, ethylene glycol, cocoanut fatty acid diethanolamide, one or more in monoglyceride.
4. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of pythium oligandrum, it is characterised in that described excipient is selected from polyvinyl alcohol, chitin, maltodextrin, trehalose, carboxymethyl starch, sodium starch glycollate, poly-(1,4-��-D-glucosan), lactose, mannitol, dextran, glycine, one or more in polylysine.
5. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of pythium oligandrum, it is characterised in that described synergist is selected from molasses, Rare Earth Lanthanum, cerium, Fructus solani xanthocarpi, fulvic acid, tea saponin, cocamido propyl betaine, ethylenediaminetetraacetic acid zinc, ethylenediaminetetraacetic acid manganese, benzene oxycarboxylic acid, one or more in fluosilicate.
6. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of pythium oligandrum, it is characterised in that described guarantor's microbial inoculum is selected from bacillus subtilis fungus extract, yeast extract, Eupatorium cannabinum L. (Eupatorium coelestinum L.) ash, rice hull ash, mugwort powder, Herba Artemisiae Annuae powder, polymyxin, zeolite, one or more in brown sugar.
7. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of pythium oligandrum, it is characterised in that described dispersant is selected from 5,5-dimethyltrimethylene carbonate methyl ester, SYN glue, polyoxyethylene alkylphenol ether, polyacrylamide, BTA, phosphoric acid five sodium, guar gum, poly-aspartate, polyglutamic acid, n-amyl alcohol, one or more in Siloxane-Oxyalkylene Copolymers.
8. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of pythium oligandrum, it is characterised in that described Plant fiber is selected from coconut fibre, cotton fiber, bombax cotton, sisal fiber, abaca fibre, flax fiber, hemp, one or more in tossa.
9. Trichoderma harzianum described in claim 1 and the application in controlling plant diseases of the pythium oligandrum composite microbial inoculum.
10. Trichoderma harzianum according to claim 9 and the application in controlling plant diseases of the pythium oligandrum composite microbial inoculum, it is characterised in that described plant disease is dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, one or more in dampinging off.
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| CN106613276A (en) * | 2017-01-09 | 2017-05-10 | 重庆市中药研究院 | Artemisia apiacea seedling cultivating method and special trichoderma atroviride fertilizer thereof |
| CN107535524A (en) * | 2017-07-04 | 2018-01-05 | 天津市华宇农药有限公司 | The bactericidal composition of biological pesticide and its application |
| US11963528B2 (en) | 2018-02-26 | 2024-04-23 | Locus Solutions Ipco, Llc | Materials and methods for control of insect pests using entomopathogenic fungi |
| US11447430B2 (en) | 2018-05-08 | 2022-09-20 | Locus Agriculture Ip Company, Llc | Microbe-based products for enhancing plant root and immune health |
| US20220022460A1 (en) * | 2018-11-29 | 2022-01-27 | Rhodia Operations | Use of guar derivatives in biofungicide compositions |
| US20220017857A1 (en) * | 2018-11-29 | 2022-01-20 | Rhodia Operations | Use of cyamopsis tetragonoloba (guar) gum for microorganisms growth |
| WO2021133630A1 (en) * | 2019-12-23 | 2021-07-01 | Locus Agriculture Ip Company, Llc | Microbe-based products for enhancing growth and phytocannabinoid content of cannabis |
| CN111996130B (en) * | 2020-09-21 | 2022-02-01 | 南京农业大学 | Biocontrol bacterium for plant root rot and application thereof |
| CN111996130A (en) * | 2020-09-21 | 2020-11-27 | 南京农业大学 | Biocontrol bacterium for plant root rot and application thereof |
| CN112772679B (en) * | 2021-02-01 | 2021-12-17 | 广西南亚热带农业科学研究所 | Biopesticide for sugarcane fungal diseases and application thereof |
| CN112772679A (en) * | 2021-02-01 | 2021-05-11 | 广西南亚热带农业科学研究所 | Biopesticide for sugarcane fungal diseases and application thereof |
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