CN105638749A - Trichoderma harzianum and polyglutamic acid compounded microbial agent and application thereof - Google Patents
Trichoderma harzianum and polyglutamic acid compounded microbial agent and application thereof Download PDFInfo
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- CN105638749A CN105638749A CN201610107212.9A CN201610107212A CN105638749A CN 105638749 A CN105638749 A CN 105638749A CN 201610107212 A CN201610107212 A CN 201610107212A CN 105638749 A CN105638749 A CN 105638749A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention discloses a trichoderma harzianum and polyglutamic acid compounded microbial agent and an application thereof. The microbial agent comprises trichoderma harzianum, polyglutamic acid and a pesticide carrier, and the pesticide carrier is selected from a wetting agent, an emulsifier, a dispersant, a stabilizer, a synergist, inorganic filler and organic filler. The composition is compounded from trichoderma harzianum and polyglutamic acid and can improve the soil and improve the crop quality; besides, the microbial agent has a better control effect on pathogenic bacteria of soil-borne diseases, has a remarkable synergistic interaction effect and can control seedling death, root rot, green wilting, dead standing, yellow wilt, butt rot, stalk rot, damping off and other common agricultural diseases.
Description
Technical field
The present invention relates to a kind of agricultural microbial inoculum, the microbial inoculum composite particularly to a kind of Trichoderma harzianum and polyglutamic acid and application thereof.
Background technology
Trichoderma can prevent and treat the plurality of plant diseases such as phytophthora root rot, banded sclerotial blight, damping-off. Trichoderma harzianum is the one in trichoderma, can be used to prevent the microbial plant diseases of cause of disease such as, sclerotinite mould by pythium spp, Rhizoctonia solani Kuhn, Fusarium spp., bread mold, post spore, Sclerotium rolfsii as a kind of biocontrol microorganisms. The mechanism of action is high concentration osmotic pressure, causes cell dehydration. As patent 201510697431.2 discloses a kind of bactericidal composition containing trichoderma harzianum and metalaxyl; Patent 201510810050.0 discloses a kind of granular formulation for killing nematode containing avilamycin and trichoderma harzianum; Patent 201510697559.9 discloses a kind of granule etc. containing trichoderma harzianum and oligochitosan.
Polyglutamic acid one water solublity, biodegradation, without the biopolymer of toxicity, molecular weight distribution, between 100kDa to 10000kDa, is extract in natto fermentation thing at first. Polyglutamic acid overflows when flooding in soil; thin film can be formed on plant root hair top layer; not only there is the function of protection root hair, the optimal transport platform of nutrient, moisture content and root hair intimate contact in soil especially, can dissolving, storage, conveying and the absorption of very efficient raising fertilizer. Stop sulfate radical, phosphate radical, oxalate and metallic element to produce precipitation, make the crop more effective absorption Soil Phosphorus of energy, calcium, magnesium and trace element. Promote the growth of crop root, strengthen disease resistance.
In the cultivation of crop, various soil-borne diseases such as dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, damping off etc. and usually to perplex farmer, solution is usually the special pesticide using various specific aim diseases, the excessive use of pesticide, not only makes soil compaction, and polluted underground water resource, cause the unsustainable development of agricultural, and disease pathogen Drug resistance strengthens, what have to increasing pesticide makes consumption, causes vicious cycle. At present, still not by composite to Trichoderma harzianum and the polyglutamic acid report preventing and treating above-mentioned agricultural disease.
Summary of the invention
It is an object of the invention to provide a kind of Trichoderma harzianum and the composite microbial inoculum of polyglutamic acid and application thereof, effectively prevent and treat various agricultural diseases.
A kind of Trichoderma harzianum and the composite microbial inoculum of polyglutamic acid, described microbial inoculum is by Trichoderma harzianum, polyglutamic acid and farm chemical carrier composition.
Described farm chemical carrier is wetting agent, emulsifying agent, dispersant, stabilizer, synergist, inorganic filler, the one in organic filler.
Described wetting agent for pulling open powder, SOPA, hydroxyethyl cellulose, polyvinylpyrrolidone, gaseous silicon oxide, Fatty alcohol polyoxyethylene polyoxypropylene ether, one or more in acrylic acid graft copolymer.
Described emulsifying agent is oleate, alkyl polyoxyethylene ether sulfonate, stearic acid, palmitic acid, alkylphenol-polyethenoxy polyethers, one or more in thermally coupled distillation columns.
Described dispersant is calcium lignosulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, multi-styrene phenylate phosphate ester, poly-second carboxylate sodium salt, alkylpolyoxyethylene phosphate ester, one or more in polyvinyl alcohol.
Described stabilizer is sodium benzoate, potassium sorbate, salicylic acid, olive oil, oleum lini, butyl glycidyl ether, one or more in cresyl glycidyl ether.
Described synergist is azone, polymeric amino acid, neem oil, fulvic acid, soybean isoflavone, camptothecine, asaricin, dry spaonin powder, one or more in ��-chloro-naphthol.
Described inorganic filler is attapulgite, Kaolin, medical stone powder, white carbon, montmorillonite, Paligorskite, one or more in sepiolite powder.
Described organic filler is coconut fibre, cotton fiber, bombax cotton, sisal fiber, abaca fibre, flax fiber, hemp, one or more in tossa.
Above-mentioned Trichoderma harzianum and the application in preventing and treating agricultural disease of the polyglutamic acid composite microbial inoculum.
Described agricultural disease includes dead Seedling, rotten, blue or green withered, vertical withered, withered and yellow withers, root-rot, and brown foot rot is dampinged off.
Compared with prior art, there is advantages that the compositions of the present invention adopts Trichoderma harzianum and polyglutamic acid composite, soil can be improved, improve crop quality, the pathogen of soil-borne disease had good prevention effect simultaneously, have obvious cooperative synergism effect, it is possible to the preventing and treating dead Seedling of crop, rotten, blue or green withered, vertical withered, withered and yellow wither, root-rot, brown foot rot, the agricultural Common Diseases such as damping off.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail, it is to be understood that protection scope of the present invention is not by the restriction of detailed description of the invention.
The reagent that following example adopt is:
Trichoderma harzianum (Trichodermaharzianum) preparation, containing trichoderma harzianum 1X108Individual/g preparation.
Polyglutamic acid, Jinan Xin Senyuan Chemical Co., Ltd. produces.
Use content:
(1) Trichoderma harzianum preparation 0.5Kg/ mu;
(2) Trichoderma harzianum preparation 1.5Kg/ mu;
(3) polyglutamic acid 0.5Kg/ mu;
(4) polyglutamic acid 1.5Kg/ mu;
(5) Trichoderma harzianum preparation 0.5Kg/ mu+polyglutamic acid 0.5Kg/ mu;
(6) Trichoderma harzianum preparation 0.5Kg/ mu+polyglutamic acid 1.5Kg/ mu;
(7) Trichoderma harzianum preparation 1.5Kg/ mu+polyglutamic acid 0.5Kg/ mu;
(8) Trichoderma harzianum preparation 1.5Kg/ mu+polyglutamic acid 1.5Kg/ mu;
(9)CK
Being Wuzhishan Area, Haikou City, Hainan Province for examination soil, soil moisture content (water: dry ground) is 29.90%, pH6.97, soil organic matter content 36.6g/kg, NH4+-N3.6mg/kg, NO3--N56.4mg/kg, available phosphorus 534.0mg/kg, available potassium 1029.0mg/kg, belong to sandy loam.
The volume increase experiment of embodiment 1 Chinese cabbage
Before Chinese cabbage is planted, being ploughed deeply in soil, uniformly apply the preparation of above-mentioned content, the nitrogenous fertilizer of same weight is used in comparison (CK), during results, calculates Chinese cabbage rate of growth;
Chinese cabbage rate of growth %:100X (processes per mu yield-comparison per mu yield)/comparison per mu yield;
Calculate rate of growth according to Colby method (1966) formula and the rate of growth with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical potentiation of mixture; N is the quantity of mixed medicament; X1Represent the rate of growth after using the 1st kind of medicament; X2Represent the rate of growth after using the 2nd kind of pharmaceutical agent; XnRepresent the rate of growth after using n medicament. When the actual rate of growth of mixture is more than theoretical rate of growth, represent potentiation; When the actual rate of growth of mixture is less than theoretical rate of growth, represent antagonism. Measurement result is as shown in table 1:
The mixed effect of increasing production to Chinese cabbage of table 1 Trichoderma harzianum preparation+polyglutamic acid
As it can be seen from table 1 Trichoderma harzianum preparation yield to Chinese cabbage mixed with the four of polyglutamic acid kinds of different amounts all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation comprises following farm chemical carrier: pull open powder 0.04g/g preparation; Enuatrol 0.04g/g preparation; Alkylpolyoxyethylene phosphoesterase 30 .02g/g preparation; Sodium benzoate 0.01g/g preparation; Azone 0.01g/g preparation; Attapulgite 0.08g/g preparation; Coconut fibre 0.46g/g preparation.
The anti-blight experiment of embodiment 2
Choosing the experimental plot that cotton spoting verticillium wilt often occurs, before cotton planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Cotton Gossypii length, to 8-9 sheet leaf, calculates cotton spoting verticillium wilt incidence rate;
Cotton spoting verticillium wilt suppression ratio %:100X (check plot cotton spoting verticillium wilt diseased plant number-treatment region cotton spoting verticillium wilt diseased plant number)/check plot cotton spoting verticillium wilt diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 2:
The mixed prevention effect to cotton spoting verticillium wilt of table 2 Trichoderma harzianum preparation+polyglutamic acid
From table 2 it can be seen that the suppression to cotton spoting verticillium wilt mixed with the four of polyglutamic acid kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation comprises following farm chemical carrier: SOPA0.01g/g preparation; Polyvinylpyrrolidone 0.01g/g preparation; Alkyl polyoxyethylene ether sulfonate 0.09g/g preparation; Polyoxyethylene polyoxypropylene base ether block copolymers 0.08g/g preparation; Potassium sorbate 0.08g/g preparation; Fulvic acid 0.01g/g preparation; Medical stone powder 0.06g/g preparation; Abaca fibre 0.24g/g preparation.
Embodiment 3 resistance to bacterial wilt is tested
Choosing the experimental plot that bacterial wilt of tomato often occurs, before tomato planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Lycopersici esculenti length, to 8-9 sheet leaf, calculates bacterial wilt of tomato incidence rate;
Bacterial wilt of tomato suppression ratio %:100X (check plot bacterial wilt of tomato diseased plant number-treatment region bacterial wilt of tomato diseased plant number)/check plot bacterial wilt of tomato diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 3:
The mixed prevention effect to bacterial wilt of tomato of table 3 Trichoderma harzianum preparation+polyglutamic acid
From table 3 it can be seen that the suppression to bacterial wilt of tomato mixed with the four of polyglutamic acid kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation comprises following farm chemical carrier: hydroxyethyl cellulose 0.04g/g preparation; Alkylphenol-polyethenoxy polyethers 0.05g/g preparation; Multi-styrene phenylate phosphoesterase 30 .04g/g preparation; Butyl glycidyl ether 0.08g/g preparation; Potassium sorbate 0.08g/g preparation; Polymeric amino acid 0.01g/g preparation; Camptothecine 0.01g/g preparation; Montmorillonite 0.06g/g preparation; Tossa 0.24g/g preparation.
The anti-damping-off experiment of embodiment 4
Choosing the experimental plot that Fructus Capsici damping-off often occurs, before pepper planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Fructus Capsici length, to 6-9 sheet leaf, calculates Fructus Capsici damping-off incidence rate;
Fructus Capsici damping-off suppression ratio %:100X (check plot Fructus Capsici damping-off diseased plant number-treatment region Fructus Capsici damping-off diseased plant number)/check plot Fructus Capsici damping-off diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 4:
The mixed prevention effect to Fructus Capsici damping-off of table 4 Trichoderma harzianum preparation+polyglutamic acid
From table 4, it can be seen that the suppression to Fructus Capsici damping-off mixed with the four of polyglutamic acid kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, synergism is obvious. Above-mentioned Trichoderma harzianum preparation comprises following farm chemical carrier: gaseous silicon oxide 0.04g/g preparation; Stearic acid 0.02g/g preparation; Poly-second carboxylate sodium salt 0.04g/g preparation; Olive oil 0.08g/g preparation; Neem oil 0.08g/g preparation; Paligorskite 0.09g/g preparation; Dry spaonin powder 0.01g/g preparation; Soybean isoflavone 0.01g/g preparation; Flax fiber 0.24g/g preparation; Sepiolite powder 0.01g/g preparation.
The anti-root rot experiment of embodiment 5
Choosing the experimental plot that fresh kidney beans root rot often occurs, before fresh kidney beans are planted, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and fresh kidney beans length, to 6-9 sheet leaf, calculates fresh kidney beans damping-off incidence rate;
Fresh kidney beans root rot suppression ratio %:100X (check plot fresh kidney beans root rot diseased plant number-treatment region fresh kidney beans root rot diseased plant number)/check plot fresh kidney beans root rot diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 5:
The mixed prevention effect to fresh kidney beans root rot of table 5 Trichoderma harzianum preparation+polyglutamic acid
As can be seen from Table 5, the suppression to fresh kidney beans root rot mixed with the four of polyglutamic acid kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, and synergism is obvious. Above-mentioned Trichoderma harzianum preparation comprises following farm chemical carrier: Fatty alcohol polyoxyethylene polyoxypropylene ether 0.04g/g preparation; Thermally coupled distillation columns acid 0.02g/g preparation; Calcium lignosulfonate 0.04g/g preparation; Salicylic acid 0.02g/g preparation; Oleum lini 0.08g/g preparation; Asaricin 0.01g/g preparation; Kaolin 0.09g/g preparation; ��-chloro-naphthol 0.01g/g preparation; Sisal fiber 0.24g/g preparation.
The anti-blight experiment of embodiment 6
Choosing the experimental plot that cotton spoting verticillium wilt often occurs, before cotton planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Cotton Gossypii length, to 8-9 sheet leaf, calculates cotton spoting verticillium wilt incidence rate;
Cotton spoting verticillium wilt suppression ratio %:100X (check plot cotton spoting verticillium wilt diseased plant number-treatment region cotton spoting verticillium wilt diseased plant number)/check plot cotton spoting verticillium wilt diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 6:
The mixed prevention effect to cotton spoting verticillium wilt of table 6 Trichoderma harzianum preparation+polyglutamic acid
As can be seen from Table 6, the suppression to cotton spoting verticillium wilt mixed with the four of polyglutamic acid kinds of different amounts of Trichoderma harzianum preparation all shows potentiation, and synergism is obvious, and effect is better than embodiment 2. Above-mentioned Trichoderma harzianum preparation comprises following farm chemical carrier: SOPA0.01g/g preparation; Polyvinylpyrrolidone 0.01g/g preparation; Alkyl polyoxyethylene ether sulfonate 0.09g/g preparation; Polyoxyethylene polyoxypropylene base ether block copolymers 0.08g/g preparation; Potassium sorbate 0.08g/g preparation; Fulvic acid 0.01g/g preparation; Medical stone powder 0.06g/g preparation; Abaca fibre 0.24g/g preparation; Barnyard grass extract 0.01g/g preparation. The extracting method of described barnyard grass extract is: takes barnyard grass stem and leaf and dries, clays into power, 70% alcoholic solution reflux, extract, of 3-5 times of weight of addition 3 times, and merging filtrate is evaporated and makes.
The anti-blight experiment of embodiment 7
Choosing the experimental plot that cotton spoting verticillium wilt often occurs, before cotton planting, ploughed deeply in soil, uniformly apply the fertilizer of above-mentioned content, comparison (CK) is blank, and Cotton Gossypii length, to 8-9 sheet leaf, calculates cotton spoting verticillium wilt incidence rate;
Cotton spoting verticillium wilt suppression ratio %:100X (check plot cotton spoting verticillium wilt diseased plant number-treatment region cotton spoting verticillium wilt diseased plant number)/check plot cotton spoting verticillium wilt diseased plant number;
Calculate suppression ratio according to Colby method (1966) formula and the suppression ratio with actual measurement compares, simply and effectively evaluate the synergy effect of mixture. Computing formula is:
E=X1��X2����Xn/100(n-1)
E is the theoretical suppression ratio of mixture; N is the quantity of mixed medicament; X1Represent the suppression ratio after using the 1st kind of medicament; X2Represent the suppression ratio after using the 2nd kind of pharmaceutical agent; XnRepresent the suppression ratio after using n medicament. When the actual suppression ratio of mixture is more than theoretical suppression ratio, represent potentiation; When the actual suppression ratio of mixture is less than theoretical suppression ratio, represent antagonism. Measurement result is as shown in table 7:
The mixed prevention effect to cotton spoting verticillium wilt of table 7 Trichoderma harzianum preparation+polyglutamic acid
As can be seen from Table 7, the suppression to cotton spoting verticillium wilt mixed with the four of polyglutamic acid kinds of different amounts of Trichoderma harzianum preparation does not all show potentiation. Above-mentioned Trichoderma harzianum preparation comprises following carrier: water 0.6g/g preparation.
The specific embodiments of the invention that are only disclosed above, but, the present invention is not limited to this, and the changes that any person skilled in the art can think of all should fall into protection scope of the present invention.
Claims (10)
1. a Trichoderma harzianum and the composite microbial inoculum of polyglutamic acid, it is characterised in that described microbial inoculum is by Trichoderma harzianum, polyglutamic acid and farm chemical carrier composition.
2. Trichoderma harzianum according to claim 1 and the composite microbial inoculum of polyglutamic acid, it is characterised in that described farm chemical carrier is wetting agent, emulsifying agent, dispersant, stabilizer, synergist, inorganic filler, the one in organic filler.
3. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterized in that, described wetting agent is for pulling open powder, SOPA, hydroxyethyl cellulose, polyvinylpyrrolidone, gaseous silicon oxide, Fatty alcohol polyoxyethylene polyoxypropylene ether, one or more in acrylic acid graft copolymer.
4. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterised in that described emulsifying agent is oleate, alkyl polyoxyethylene ether sulfonate, stearic acid, palmitic acid, alkylphenol-polyethenoxy polyethers, one or more in thermally coupled distillation columns.
5. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterized in that, described dispersant is calcium lignosulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, multi-styrene phenylate phosphate ester, poly-second carboxylate sodium salt, alkylpolyoxyethylene phosphate ester, one or more in polyvinyl alcohol.
6. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterised in that described stabilizer is sodium benzoate, potassium sorbate, salicylic acid, olive oil, oleum lini, butyl glycidyl ether, one or more in cresyl glycidyl ether.
7. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterised in that described synergist is azone, polymeric amino acid, neem oil, fulvic acid, soybean isoflavone, camptothecine, asaricin, dry spaonin powder, one or more in ��-chloro-naphthol.
8. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterised in that described inorganic filler is attapulgite, Kaolin, medical stone powder, white carbon, montmorillonite, Paligorskite, one or more in sepiolite powder.
9. Trichoderma harzianum according to claim 2 and the composite microbial inoculum of polyglutamic acid, it is characterised in that described organic filler is coconut fibre, cotton fiber, bombax cotton, sisal fiber, abaca fibre, flax fiber, hemp, one or more in tossa.
10. Trichoderma harzianum described in claim 1 and the application in preventing and treating agricultural disease of the polyglutamic acid composite microbial inoculum.
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Cited By (5)
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| CN106342908A (en) * | 2016-08-04 | 2017-01-25 | 王永强 | Compound microbial agent composition capable of reducing continuous cropping obstacles for crops |
| CN106745814A (en) * | 2016-12-31 | 2017-05-31 | 新昌县田野泉养殖技术开发有限公司 | Compound improver of water quality for aquaculture and preparation method thereof |
| WO2019184259A1 (en) * | 2018-03-25 | 2019-10-03 | 江苏辉丰生物农业股份有限公司 | Medical fertilizer composition containing potassium phosphite and polyglutamic acid |
| CN113475533A (en) * | 2021-06-17 | 2021-10-08 | 杨凌未来中科环保科技有限公司 | Agricultural microbial agent containing polyglutamic acid, preparation method and application thereof |
| CN114868770A (en) * | 2022-06-25 | 2022-08-09 | 石河子大学 | Agricultural composition containing bactericide and gamma-polyglutamic acid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106342908A (en) * | 2016-08-04 | 2017-01-25 | 王永强 | Compound microbial agent composition capable of reducing continuous cropping obstacles for crops |
| CN106745814A (en) * | 2016-12-31 | 2017-05-31 | 新昌县田野泉养殖技术开发有限公司 | Compound improver of water quality for aquaculture and preparation method thereof |
| WO2019184259A1 (en) * | 2018-03-25 | 2019-10-03 | 江苏辉丰生物农业股份有限公司 | Medical fertilizer composition containing potassium phosphite and polyglutamic acid |
| CN113475533A (en) * | 2021-06-17 | 2021-10-08 | 杨凌未来中科环保科技有限公司 | Agricultural microbial agent containing polyglutamic acid, preparation method and application thereof |
| CN114868770A (en) * | 2022-06-25 | 2022-08-09 | 石河子大学 | Agricultural composition containing bactericide and gamma-polyglutamic acid |
| CN114868770B (en) * | 2022-06-25 | 2024-03-19 | 石河子大学 | Agricultural composition containing microbial inoculum and gamma-polyglutamic acid |
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Application publication date: 20160608 |