CN114868770B - Agricultural composition containing microbial inoculum and gamma-polyglutamic acid - Google Patents

Agricultural composition containing microbial inoculum and gamma-polyglutamic acid Download PDF

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CN114868770B
CN114868770B CN202210731764.2A CN202210731764A CN114868770B CN 114868770 B CN114868770 B CN 114868770B CN 202210731764 A CN202210731764 A CN 202210731764A CN 114868770 B CN114868770 B CN 114868770B
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gamma
polyglutamic acid
bacillus amyloliquefaciens
streptomycete
tomato
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CN114868770A (en
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张国强
王秀爽
王春娟
赵婧
韩业明
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Shihezi University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid, and the effective components of the agricultural composition comprise streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid. The ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:0.5-2:0.4-5, and the total concentration of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 is 1 multiplied by 10 10 ‑5×10 10 cfu/mL. The agricultural composition provided by the invention is used for seed treatment, has excellent effects in preventing and treating tomato leaf mold and tomato early blight, and plays a role in coordination and synergy after the combination of the active ingredients.

Description

Agricultural composition containing microbial inoculum and gamma-polyglutamic acid
Technical Field
The invention belongs to the technical field of pesticide compounding, relates to an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid, and in particular relates to an agricultural composition with a prevention and treatment effect on fungal diseases.
Background
Tomato leaf mold is caused by Cladosporium flavum and belongs to the genus Phaeolomyces of the subdivision Deuteromycotina. Tomato leaf mold seriously damages tomato leaves and fruits, frequently occurring in greenhouse-planted tomatoes. The tomato leaf mold germ can overwinter in the disease residue with mycelium or mycelium block, or can attach to the surface of seed or the mycelium is hidden in the seed coat for overwintering. Seed from seed-borne bacteria can also cause initial infestation. The tomato leaf mold is re-infected for a plurality of times, and the pathogenic bacteria germinate to form a disease spot, and when the environmental conditions are proper, a large number of conidia are generated on the disease spot, so that the continuous re-infection is carried out.
The pathogen of tomato early blight is Alternaria solani, belonging to the fungus of the phylum half-known fungus. Tomato early blight host tomato, eggplant, capsicum, potato, etc., which is the main disease tomato. Tomato early blight often causes leaf fall, fruit fall and branch break, and the disease reduces yield by more than 30%, especially serious disease in greenhouse. Distribution occurs across the country. Early blight of tomato can occur in seedling stage and adult stage of tomato, and is the most easily occurring part of leaf, stem, flower, fruit, etc., and branch part of leaf and stem.
Gamma-polyglutamic acid (gamma-PGA), which is a substance nontoxic to human body and environment with a polymerization degree of about 1000-15000, is prepared by polymerizing glutamic acid with left and right optical rotation as unit body and amide bond on gamma-position to obtain homogeneous polypeptide. The gamma-PGA has excellent water solubility, super-strong adsorptivity and biodegradability, and the degradation product is pollution-free glutamic acid, so that the gamma-PGA is an excellent environment-friendly high polymer material. The gamma-PGA can be used as a water-retaining agent, a heavy metal ion adsorbent, a flocculating agent, a slow release agent, a drug carrier and the like, and has great commercial value and social value in industries such as cosmetics, environmental protection, foods, medicines, agriculture, desert control and the like. When the gamma-PGA is flooded in the soil, a layer of film is formed on the surface layer of plant root hairs, so that the fertilizer has the function of protecting the root hairs, is an optimal conveying platform for closely contacting nutrients and moisture in the soil with the root hairs, and can effectively improve the dissolution, storage, conveying and absorption of the fertilizer. The gamma-PGA can prevent sulfate radical, phosphate radical, oxalate radical and metal element from producing precipitation action, so that crops can effectively absorb phosphorus, calcium, magnesium and trace elements in soil. The gamma-PGA can promote the growth of crop root system and strengthen disease resistance.
CN106434499B discloses an actinomycete strain KN37 and application thereof, and the actinomycete strain KN37 obtained by separation is identified as streptomyces by microscopic observation and 16S rDNA sequence analysis, and the preservation number is cgmccno.13160; CN106434499B discloses that the fermentation liquor of strain KN37 has good inhibitory effect on a plurality of plant pathogenic bacteria such as botrytis cinerea, early blight of tomato, fusarium wilt of cotton, and leaf mold of tomato, and can be used for preparing microbial agents for preventing and treating crop diseases. CN103814892B discloses a bacillus amyloliquefaciens water dispersible granule comprising effective active ingredients, an auxiliary agent and a filler, wherein the effective active ingredients are bacillus amyloliquefaciens strain B1619, and the bacterial content is 5 multiplied by 10 10 cfu/g-30×10 10 cfu/g; the auxiliary agent comprises 0.5-6% of binder, 0.5-6% of dispersant and 1-10% of disintegrating agent, and the balance of filler by weight; the invention is a granule, can be used together with fertilizer to prevent and treat soil-borne diseases, can also be used for spraying, and has the advantages of high product density, small volume and easy package and transportation. CN108271785a discloses a biopesticide produced by fermenting bean curd yellow water and a preparation method thereof, wherein the biopesticide is obtained by fermenting bean curd yellow slurry water serving as a fermentation raw material by microorganisms, and a fermentation product contains gamma-polyglutamic acid and antibacterial peptide; the biological pesticide has obvious antibacterial effect on plant pathogenic fungi. The above patents respectively provide bacteriostatic applications using one of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid as an active ingredient, but the above patents are not ideal for preventing and treating tomato leaf mold or tomato early blight when being used alone.
The inventor of the patent application explores an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid through intensive research, and the composition has excellent effects on preventing and treating tomato leaf mold and tomato early blight, especially for seed treatment. At present, no document for preventing and treating tomato leaf mold or tomato early blight by combining streptomyces KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid is known, and the beneficial effect of the composition for plant growth regulation is difficult to be known by a person of ordinary skill in the art.
Disclosure of Invention
There are many reports on the prevention and treatment of fungal diseases by using streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid. At present, no report on the compounding of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid for preventing and treating fungal diseases is found.
Based on the above, the invention provides an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid, wherein the effective components of the agricultural composition comprise streptomyces KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid; the ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:0.5-2:0.4-5 in terms of mass ratio; the total concentration of the streptomycete KN37 and the bacillus amyloliquefaciens B1619 viable bacteria is 1 multiplied by 10 10 -5×10 10 cfu/mL。
Those skilled in the art can easily know that the pesticide effect of the compound bactericidal composition is improved, the occurrence of drug resistance is avoided, the bactericidal spectrum is expanded, and a plurality of pesticides are mostly used in combination or combined use, but whether the bactericidal effect after the combination or combined use of the pesticides is synergistic or not is difficult to expect because the inherent mechanism of each component is unknown. The inventor of the patent researches through intensive experiments, and comprehensively evaluates the effects shown by field experiments, and the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention has excellent prevention and control effects on fungal diseases, especially tomato leaf mold or tomato early blight, and is better than the use effect of a single dose.
As one of the preferred embodiments of the technical scheme of the invention, the ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:0.8-1.4:1-3 in terms of mass ratio. Within the range of the proportion, the two components show excellent synergistic effect.
As one of the preferred embodiments of the technical scheme, the ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:1-1.2:1.5-2 in terms of mass ratio. Within the range of the proportion, the two components show excellent synergistic effect.
As one of the preferred embodiments of the technical scheme, the ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:1.1:1.8 in terms of mass ratio. Within the range of the proportion, the two components show excellent synergistic effect.
Still another object of the present invention is to provide the use of said agricultural composition for the control of fungal diseases. The agricultural composition or a preparation containing the agricultural composition is used for preventing and treating fungal diseases; the fungal diseases include tomato leaf mold and tomato early blight.
According to the actual application requirement, the agricultural composition provided by the invention can be prepared into a preparation formulation which is convenient for agricultural application, such as one of seed dressing agent, seed soaking agent, suspending agent, wettable powder, water dispersible granule and the like according to a method known by a person skilled in the art. Those skilled in the art will readily appreciate that the formulation system contains, in addition to the active ingredient, adjuvants required for the formulation of the pesticide formulation. The auxiliary agent can be one or a mixture of a plurality of solvents, emulsifying agents, wetting agents, stabilizing agents, dispersing agents, thickening agents, pH regulators, defoamers, antifreezing agents, fillers and the like. The adjuvants of the present invention may be known substances, such as various adjuvants commonly used in pesticide preparations, and may be changed according to different situations, and are not particularly limited. Preferably, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention adopts one of seed dressing and seed soaking to treat seeds.
Compared with the prior art, the agricultural composition provided by the invention has at least the following advantages or beneficial effects:
compared with the prior art, the main contribution of the invention is to provide an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid and application thereof in agriculture, wherein the active ingredients of the agricultural composition are composed of streptomyces KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid. The invention combines streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid, and discovers that the composition has outstanding performance in preventing and treating fungal diseases, especially tomato leaf mold and tomato early blight, in the proportion range provided by the invention. The invention realizes the technical effect of the compound synergy of the streptomycete KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid, and the agricultural composition can improve the crop yield.
Detailed Description
The following describes the technical aspects of the present invention with reference to examples, but the present invention is not limited to the following examples.
The experimental methods and the detection methods described in the following examples are all conventional methods unless otherwise specified; the agents and materials are commercially available unless otherwise specified. The preservation number of Streptomyces KN37 in the following examples is CGMCC No.13160.
Example 1
The example provides an experiment for controlling tomato leaf mold by an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid.
The tomato variety to be tested is L-402 tomato, which is introduced from the institute of gardening, the national academy of agricultural sciences, liaoning province. The test selects a cultivation mode of a plastic greenhouse protected area, the tomato leaf mold is serious before the test field, and the water and fertilizer management is unified. The test was conducted in a test group 1-7, a single dose group 1-13, and a blank, and the effective components of each test group were as shown in Table 1. Test group 1-test group 7 Streptomyces KN37 powder, bacillus amyloliquefaciens B1619 powder, and gamma-polyglutamic acid were mixed according to the ratios shown in Table 1. The single dose group 1-13 are single doses of each effective component with different dilution factors. The blank control group is clear water. The effective viable count of the streptomycete KN37 bacterial powder and the bacillus amyloliquefaciens B1619 bacterial powder used for identification before dosage is 1 multiplied by 10 10 -5×10 10 cfu/mL. Tomato seeds were diluted as the active ingredients shown in table 1. For example, test group 1 was a diluted solution prepared by dissolving 1g of Streptomyces KN37 powder, 0.5g of Bacillus amyloliquefaciens B1619 powder, and 0.4g of gamma-polyglutamic acid in 50mL of water. Tomato seeds were seed-dressed with a 0.5% seed weight dilution. Each test cell is arranged randomly, and each cell is 5m 2 Each test set was 3And (5) repeating. Two months after the first occurrence of tomato leaf mold in the test field, 5 points are adopted to survey the number of diseased leaves in each cell, 6 plants are surveyed at each point, and the test results are shown in table 1.
The leaf mold classification criteria are as follows:
level 0: no disease spots; stage 1: the area of the disease spots accounts for less than the whole leaf area percent; 3 stages: the area of the lesion accounts for 6% -10% of the whole leaf area; 5 stages: the area of the lesion accounts for 11% -20% of the whole leaf area; 7 stages: the area of the lesion accounts for 21% -50% of the whole leaf area; stage 9: the area of the disease spots accounts for more than 51% of the whole leaf area.
Disease index = [ (leaf number of each stage×relative stage number)/(total leaf number of investigation×9) ]×100
Control effect (%) = [ (blank control disease finger-drug treatment disease finger)/blank control disease finger ] ×100
The control effect of the mixed active ingredients is tested by adopting a Growing method, and the formula is E 0 ＝X+[Y×(100-X)/100]，E 0 The theoretical control effect of the composition (A+B+C) when the dosage is (P+Q+R), X represents the control effect of the medicament A when the dosage is P, Y represents the sum of the control effect of the medicament B when the dosage is Q and the control effect of the medicament C when the dosage is R; e represents the actual control effect of the treatment of the composition. When E-E 0 >10% is synergistic effect; when E-E 0 <-10% antagonism; when E-E 0 Values between + -10% are additive.
TABLE 1 control of tomato leaf mold by agricultural compositions containing microbial inoculum and gamma-polyglutamic acid
As shown in Table 1, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention can effectively realize the control of tomato leaf mold in a certain proportioning range, and the control effect is higher than that of each single agent, and the synergistic effect is obvious. Particularly, when the proportion of the streptomyces KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1:1.1:1.8, the synergistic effect is the highest, and the control effect on the tomato leaf mold is 87.65%.
Example 2
The present example provides an experiment for controlling early blight of tomato and improving the yield of tomato by using an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid.
The tomato variety to be tested is L-402 tomato, which is introduced from the institute of gardening, the national academy of agricultural sciences, liaoning province. And (3) selecting a cultivation mode of a plastic greenhouse protected area in a test, and uniformly managing water and fertilizer in a test field. The test is provided with a test group 1-7, a single dose group 1-3, a positive control group and a blank control group. Test group 1 to test group 7 are agricultural compositions containing a microbial agent and gamma-polyglutamic acid in different proportions and the total dosage is the same, and the specific proportions are shown in table 2. The single dose groups 1-3 are respectively single doses of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid. The positive control group was 0.5% amino oligosaccharin. The blank control group is clear water. The effective viable count of the streptomycete KN37 bacterial powder and the bacillus amyloliquefaciens B1619 bacterial powder used for identification before dosage is 1 multiplied by 10 10 -5×10 10 cfu/mL. Test group 1-test group 7 the specific embodiment is that Streptomyces KN37 bacterial powder, bacillus amyloliquefaciens B1619 bacterial powder and gamma-polyglutamic acid are mixed according to the proportion shown in Table 2, and the total mass is 1g, and diluted with 100mL of clear water to obtain the corresponding diluent. The specific implementation scheme of the single dose group 1-single dose group 3 is that 1g of one of streptomycete KN37 bacterial powder, bacillus amyloliquefaciens B1619 bacterial powder and gamma-polyglutamic acid is diluted with 100mL of clear water to obtain corresponding diluent. Soaking seeds for 20min by using liquid medicine (1 g/mL diluent of test group 1-7, 1-3 of single dose, 0.5% amino oligosaccharin of positive control group and clear water of blank control group), soaking seeds for 10min at 50-55 ℃ after cleaning the liquid medicine, cooling to 30 ℃ with cold water, soaking seeds, and taking out the seeds for germination accelerating and sowing.
Each test cell is arranged randomly, and each cell is 5m 2 Each set of experiments was replicated 3 times. Two months after the first occurrence of the early blight of tomatoes in the test field, 5 points are adopted to survey the number of diseased leaves in each district, 6 plants are surveyed at each point,the disease classification, disease index and statistical method of the control effect are the same as in example 1. After the tomatoes are fully mature, counting the tomato yield of each district. The statistical results are shown in Table 2.
TABLE 2 Effect of agricultural compositions containing microbial Agents and gamma-polyglutamic acid on early blight control of tomato and on tomato yield
As shown in Table 2, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention can effectively realize the prevention and treatment of tomato early blight within a certain proportioning range, and the prevention and treatment effect is higher than that of each single agent with equal dosage, and the synergistic effect is obvious. When the proportion of the streptomycete KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1:1.1:1.8, the synergistic effect is highest, and the control effect on the tomato early blight is 73.20%. As can be obtained from Table 2, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention can obviously improve the tomato yield, and the highest tomato yield reaches 5727.12kg/hm 2 The highest yield is 20.50%.
The present invention may be better implemented as described above, and the above examples are merely illustrative of preferred embodiments of the present invention and not intended to limit the scope of the present invention, and various changes and modifications made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the present invention without departing from the spirit of the design of the present invention.

Claims (4)

1. An agricultural composition containing a microbial inoculum and gamma-polyglutamic acid is characterized in that the effective components comprise streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid;
the streptomycete KN37 is streptomycete KN37 bacterial powder, and the bacillus amyloliquefaciens B1619 is bacillus amyloliquefaciens B1619 bacterial powder;
the effective viable count of the streptomycete KN37 bacterial powder and the bacillus amyloliquefaciens B1619 bacterial powder is 1 multiplied by 10 10 -5×10 10 cfu/mL；
The ratio of the streptomycete KN37 bacterial powder to the bacillus amyloliquefaciens B1619 bacterial powder to the gamma-polyglutamic acid is 1:1.1:1.8 in terms of mass ratio.
2. The use of an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid as set forth in claim 1 for controlling fungal diseases, wherein the fungal diseases are tomato leaf mold and tomato early blight.
3. The use according to claim 2, characterized in that the agricultural composition comprising a microbial agent and gamma-polyglutamic acid is used for seed treatment.
4. Use according to claim 3, characterized in that the seed treatment comprises soaking or dressing.
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