CN114868770A - Agricultural composition containing bactericide and gamma-polyglutamic acid - Google Patents
Agricultural composition containing bactericide and gamma-polyglutamic acid Download PDFInfo
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- CN114868770A CN114868770A CN202210731764.2A CN202210731764A CN114868770A CN 114868770 A CN114868770 A CN 114868770A CN 202210731764 A CN202210731764 A CN 202210731764A CN 114868770 A CN114868770 A CN 114868770A
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- polyglutamic acid
- bacillus amyloliquefaciens
- agricultural composition
- tomato
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- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
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- 241000222291 Passalora fulva Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid, which comprises the effective components of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid. The weight ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1: 0.5-2: 0.4-5, and the total concentration of the viable bacteria of the streptomycete KN37 and the bacillus amyloliquefaciens B1619 is 1 x 10 10 ‑5×10 10 cfu/mL. The agricultural composition provided by the invention is used for seed treatmentTherefore, the composition shows excellent effect in controlling tomato leaf mold and tomato early blight, and the active ingredients play a role in coordination and synergism after being combined.
Description
Technical Field
The invention belongs to the technical field of pesticide compounding, relates to an agricultural composition containing a fungicide and gamma-polyglutamic acid, and particularly relates to an agricultural composition with a control effect on fungal diseases.
Background
The tomato leaf mold is caused by cladosporium fulvum and belongs to the hypo-nyctalopsis. Tomato leaf mold seriously harms tomato leaves and fruits, frequently occurring in tomatoes planted in greenhouses. The tomato leaf mold pathogen overwintering with mycelium or hypha block in the disease residue, or attaching conidium on the seed surface or the hypha latent in the seed coat. Sowing seeds with bacteria can also cause primary infection. The tomato leaf mold is re-infected for many times, the germs germinate to form disease spots, and when the environmental conditions are proper, a large number of conidia are generated on the disease spots and are re-infected continuously.
The pathogen of tomato early blight is alternaria solani, which belongs to fungi of deuteromycotina. The tomato early blight host comprises tomato, eggplant, hot pepper, potato and the like, and the tomato is mainly diseased. Early blight of tomato often causes fallen leaves, fallen fruits and broken branches, and the yield is reduced by more than 30% due to the early blight, especially the early blight in greenhouses and greenhouses. Distribution occurs across the country. Tomato early blight can occur in seedling stage and adult stage of tomato, and is most likely to occur at parts of leaves, stems, flowers, fruits and the like, and branches of leaves, stems and leaves.
Gamma-polyglutamic acid (gamma-PGA), named natto gum, is homogeneous polypeptide (Homo-polypeptide) polymerized with the amino acid bond in gamma position and with polymerization degree of about 1000-15000 and no toxicity to human body and environment. The gamma-PGA has excellent water solubility, super-strong adsorbability and biodegradability, and the degradation product is pollution-free glutamic acid, so that the gamma-PGA is an excellent environment-friendly high polymer material. The gamma-PGA can be used as a water-retaining agent, a heavy metal ion adsorbent, a flocculating agent, a slow release agent, a drug carrier and the like, and has great commercial value and social value in industries such as cosmetics, environmental protection, food, medicine, agriculture, desert control and the like. When the gamma-PGA is flooded in soil, a layer of film is formed on the surface layer of plant root hair, so that the fertilizer has the function of protecting the root hair, is an optimal conveying platform for closely contacting nutrients and water in the soil with the root hair, and can effectively improve the dissolution, storage, conveying and absorption of the fertilizer. Gamma-PGA can prevent sulfate radicals, phosphate radicals, oxalate radicals and metal elements from generating precipitation, so that crops can absorb phosphorus, calcium, magnesium and trace elements in soil more effectively. The gamma-PGA can promote the growth of crop root system and strengthen disease resistance.
CN106434499B discloses an actinomycete strain KN37 and application thereof, wherein the actinomycete strain KN37 obtained by separation is identified as streptomyces through microscopic observation and 16S rDNA sequence analysis, and the preservation number is CGMCC No. 13160; CN106434499B discloses that the fermentation liquor of the strain KN37 has good inhibition effect on various plant pathogenic bacteria such as botrytis cinerea, early blight of tomato, fusarium wilt of cotton, leaf mold of tomato and the like, and can be used for preparing microbial agents for preventing and treating crop diseases. CN103814892B discloses a Bacillus amyloliquefaciens water dispersible granule, which comprises effective active ingredients, an auxiliary agent and a filler, wherein the effective active ingredients are Bacillus amyloliquefaciens strain B1619 with the bacterial content of 5 multiplied by 10 10 cfu/g-30×10 10 cfu/g; the auxiliary agent comprises a binder, a dispersing agent and a disintegrating agent, wherein the binder accounts for 0.5-6 wt%, the dispersing agent accounts for 0.5-6 wt%, the disintegrating agent accounts for 1-10 wt%, and the balance is filler; the invention is a granule, can be used together with fertilizer to prevent and treat soil-borne diseases, can also be used for spraying, and has the advantages of high product density, small volume and easy packaging and transportation. CN108271785A discloses a biological pesticide produced by fermenting bean curd yellow water and a preparation method thereof, wherein the biological pesticide is obtained by fermenting microorganisms by taking bean curd yellow serofluid as a fermentation raw material, and a fermentation product contains gamma-polyglutamic acid and antibacterial peptide; the biological pesticide has obvious bacteriostasis effect on plant pathogenic fungi. The patents respectively provide the bacteriostatic application of taking one of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid as an active ingredient, but the patent is not ideal for preventing and treating tomato leaf mold or tomato early blight by being used alone.
Through intensive research, the inventor of the patent application discovers an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid, and the composition shows excellent effects on preventing and treating tomato leaf mold and tomato early blight, particularly for seed treatment. At present, the literature of combining streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid for preventing and controlling tomato leaf mold or tomato early blight is not available, and the beneficial effect of the composition for plant growth regulation is difficult to know by the ordinary skilled person in the art.
Disclosure of Invention
The use of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid for preventing and treating fungal diseases has been reported in many documents. At present, no report related to the compounding of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid for preventing and treating fungal diseases is found.
Based on the technical scheme, the invention provides an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid, and the effective components of the agricultural composition comprise streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid; the mass ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1: 0.5-2: 0.4-5; the total concentration of the live bacteria of the streptomycete KN37 and the bacillus amyloliquefaciens B1619 is 1 multiplied by 10 10 -5×10 10 cfu/mL。
The ordinary skilled in the art can easily know that the pesticide effect of the compound bactericidal composition is improved, the occurrence of drug resistance is avoided, the bactericidal spectrum is expanded, and the combination use or the combined use of multiple pesticides is mostly adopted, but due to the unclear internal mechanism of each component, whether the bactericidal effect of the combination use or the combined use of the multiple pesticides is synergistic is difficult to expect. Through deep experimental research by the inventor team, according to comprehensive evaluation of effects shown in field tests, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention has excellent control effects on fungal diseases, particularly tomato leaf mold or tomato early blight, and is superior to the using effect of a single agent.
According to a preferred embodiment of the technical scheme, the ratio of the streptomycete KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1: 0.8-1.4: 1-3 in mass ratio. Within the range of the mixture ratio, the two show excellent synergistic effect.
According to a preferred embodiment of the technical scheme, the ratio of the streptomycete KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1: 1-1.2: 1.5-2 by mass ratio. Within the range of the mixture ratio, the two show excellent synergistic effect.
As one of the preferable embodiments of the technical scheme of the invention, the ratio of the streptomycete KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1:1.1:1.8 by mass ratio. Within the range of the mixture ratio, the two show excellent synergistic effect.
Still another object of the present invention is to provide the use of the agricultural composition for the control of fungal diseases. The agricultural composition or the preparation containing the agricultural composition is used for preventing and controlling fungal diseases; the fungal diseases comprise tomato leaf mold and tomato early blight.
According to the actual application requirement, the agricultural composition provided by the invention can be prepared into a preparation formulation convenient for agricultural application according to a method known by a person skilled in the art, such as one of seed dressing agent, seed soaking agent, suspending agent, wettable powder, water dispersible granule and the like. Those skilled in the art will readily appreciate that in addition to the active ingredient, the formulation system may contain adjuvants necessary for the formulation of pesticidal formulations. The auxiliary agent can be one or a mixture of more of a solvent, an emulsifier, a wetting agent, a stabilizer, a dispersant, a thickener, a pH regulator, a defoaming agent, an antifreezing agent, a filler and the like. The adjuvants of the present invention may be known substances, such as various adjuvants commonly used in pesticide preparations, and may be varied according to circumstances, and are not particularly limited. Preferably, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention adopts one of seed dressing and seed soaking to treat seeds.
Compared with the prior art, the agricultural composition has at least the following advantages or beneficial effects:
compared with the prior art, the invention mainly contributes to providing an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid and application thereof in agriculture, wherein the active ingredients of the agricultural composition are composed of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid. The streptomyces KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid are compounded, and the compound is found to have outstanding performance in preventing and treating fungal diseases, particularly tomato leaf mold and tomato early blight, within the proportioning range provided by the invention. The invention realizes the technical effect of compounding and synergy of the streptomycete KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid, and the agricultural composition can improve the crop yield.
Detailed Description
The following examples are given to illustrate the technical aspects of the present invention, but the present invention is not limited to the following examples.
The experimental methods and the detection methods described in the following examples are all conventional methods unless otherwise specified; the agents and materials are commercially available, unless otherwise specified. The preservation number of the streptomycete KN37 strain in the following examples is CGMCC No. 13160.
Example 1
The embodiment provides a test for controlling tomato leaf mold by using an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid.
The tested tomato variety is L-402 tomato, introduced by the horticultural research institute of academy of agricultural sciences of Liaoning province. A plastic greenhouse protected area cultivation mode is selected in the test, the tomato leaf mold is serious in the test field, and water and fertilizer management is unified. The test is provided with a test group 1-a test group 7, a single-dose group 1-a single-dose group 13 and a blank control group, and the distribution ratio of the effective components of each test group is shown in table 1. Test group 1 to test group 7, streptomycete KN37 bacterial powder, bacillus amyloliquefaciens B1619 bacterial powder and gamma-polyglutamic acid were mixed according to the mixture ratio described in table 1. The single-dose group 1-the single-dose group 13 are single doses of each effective component with different dilution times. The blank control group was clear water. The effective viable count of the streptomycete KN37 bacterial powder and the bacillus amyloliquefaciens B1619 bacterial powder used for identification before medicine preparation is 1 multiplied by 10 10 -5×10 10 cfu/mL. Tomato seeds were diluted with the active ingredients shown in table 1. Illustratively, in test group 1, 1g of streptomyces KN37 bacteria powder, 0.5g of bacillus amyloliquefaciens B1619 bacteria powder and 0.4g of gamma-polyglutamic acid are mixed and dissolved in 50mL of water to obtain a dilution liquid with the ratio. Tomato seeds were diluted with 0.5% by weight of the seedAnd (4) carrying out seed dressing treatment on the seeds. The test cells were randomly arranged at 5m per cell 2 Each set of experiments was set to 3 replicates. Two months after the test field first shows tomato leaf mold disease, 5 points are adopted to investigate the number of diseased leaves in each plot, 6 plants are investigated at each point, and the test results are shown in table 1.
The leaf mold grading criteria are as follows:
level 0: no disease spots; level 1: the area of the lesion spots accounts for less than the whole leaf area; and 3, level: the lesion area accounts for 6 to 10 percent of the whole leaf area; and 5, stage: the lesion area accounts for 11 to 20 percent of the whole leaf area; and 7, stage: the lesion area accounts for 21-50% of the whole leaf area; and 9, stage: the lesion area accounts for more than 51 percent of the whole leaf area.
Disease index ═ number of diseased leaves at each stage x relative stage number)/(total number of investigated leaves x 9) ] × 100
Control effect (%) [ (blank control disease means-medicament treatment disease means)/blank control disease means ] × 100
The prevention and control effect of the mixed active components is tested by adopting a Growing method, and the formula is E 0 ＝X+[Y×(100-X)/100]，E 0 Represents the theoretical prevention effect of the composition (A + B + C) when the dosage is (P + Q + R), X represents the prevention effect of the medicament A when the dosage is P, and Y represents the sum of the prevention effect of the medicament B when the dosage is Q and the prevention effect of the medicament C when the dosage is R; e represents the actual control effect of the composition treatment. When E-E 0 >10% of the composition is synergistic effect; when E-E 0 <-10% is antagonism; when E-E 0 Values within. + -. 10% are additive effects.
TABLE 1 control of tomato leaf mold by agricultural compositions containing microbial inoculum and gamma-polyglutamic acid
As shown in Table 1, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid can effectively realize the prevention and control of the tomato leaf mold within a certain proportion range, the prevention and control effect is higher than that of each single agent, and the synergistic effect is obvious. Particularly, when the ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:1.1:1.8, the synergistic effect is highest, and the control effect on tomato leaf mold is 87.65%.
Example 2
The embodiment provides a test for preventing and treating tomato early blight and improving tomato yield by using an agricultural composition containing a microbial inoculum and gamma-polyglutamic acid.
The tested tomato variety is L-402 tomato, introduced by the horticultural research institute of academy of agricultural sciences of Liaoning province. And (4) selecting a plastic greenhouse protected area cultivation mode in the test, and uniformly managing water and fertilizer in the test field. The test is provided with a test group 1-a test group 7, a single-dose group 1-a single-dose group 3, a positive control group and a blank control group. The test groups 1 to 7 are agricultural compositions containing the microbial inoculum and the gamma-polyglutamic acid with different mixture ratios and the same total dosage, and the specific mixture ratio is shown in table 2. The single-dose groups 1 to 3 are single doses of streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid respectively. The positive control group was 0.5% amino-oligosaccharin. The blank control group was clear water. The effective viable count of the streptomycete KN37 bacterial powder and the bacillus amyloliquefaciens B1619 bacterial powder used for identification before medicine preparation is 1 multiplied by 10 10 -5×10 10 cfu/mL. Test group 1-test group 7 according to the specific embodiment, streptomycete KN37 bacterial powder, bacillus amyloliquefaciens B1619 bacterial powder and gamma-polyglutamic acid are mixed according to the mixture ratio shown in table 2 to prepare 1g of total mass, and the total mass is diluted with 100mL of clear water to obtain corresponding diluent. The specific embodiment of the single-dose group 1-3 is that 1g of streptomycete KN37 strain powder, bacillus amyloliquefaciens B1619 strain powder and one of gamma-polyglutamic acid are respectively diluted with 100mL of clear water to obtain corresponding diluents. Soaking seeds for 20min by using liquid medicines (1 g/mL diluent for the test group 1-test group 7 and the single-dose group 1-single-dose group 3, 0.5% amino-oligosaccharin for the positive control group and clear water for the blank control group) prepared by each group, then cleaning the seeds, soaking the seeds in the liquid medicines at the water temperature of 50-55 ℃ for 10min, then adding cold water, cooling to 30 ℃ for soaking the seeds, taking out the seeds, accelerating germination and sowing.
The test cells were randomly arranged at 5m per cell 2 Each set of experiments was set to 3 replicates. To be testedTwo months after the tomato early blight appears in the field for the first time, 5 points are adopted to investigate the number of diseased leaves in each cell, 6 strains are investigated at each point, and the statistical method of disease classification, disease index and prevention and treatment effect is the same as that in example 1. And after the tomatoes are completely ripe, counting the tomato yield of each cell. The statistical results are shown in table 2.
TABLE 2 Effect of agricultural compositions containing microbial inoculum and gamma-polyglutamic acid on prevention of early blight of tomato and on tomato yield
As shown in Table 2, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention can effectively realize the prevention and treatment of the tomato early blight within a certain proportion range, the prevention and treatment effect is higher than that of each single agent with equal dosage, and the synergistic effect is obvious. When the ratio of the streptomycete KN37 to the bacillus amyloliquefaciens B1619 to the gamma-polyglutamic acid is 1:1.1:1.8, the synergistic effect is highest, and the control effect on tomato early blight reaches 73.20%. As can be seen from Table 2, the agricultural composition containing the microbial inoculum and the gamma-polyglutamic acid provided by the invention can obviously improve the tomato yield, and the highest tomato yield reaches 5727.12kg/hm 2 The yield is increased by 20.50 percent at most.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (10)
1. An agricultural composition containing a fungicide and gamma-polyglutamic acid is characterized in that the effective components comprise streptomycete KN37, bacillus amyloliquefaciens B1619 and gamma-polyglutamic acid.
2. The agricultural composition as claimed in claim 1, wherein the weight ratio of the streptomyces KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1: 0.5-2: 0.4-5;
the total concentration of the live bacteria of the streptomycete KN37 and the bacillus amyloliquefaciens B1619 is 1 multiplied by 10 10 -5×10 10 cfu/mL。
3. The agricultural composition as claimed in claim 2, wherein the weight ratio of the streptomyces KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1: 0.8-1.4: 1-3.
4. The agricultural composition as claimed in claim 3, wherein the weight ratio of the streptomyces KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1: 1-1.2: 1.5-2.
5. The agricultural composition as claimed in claim 4, wherein the weight ratio of the streptomyces KN37, the bacillus amyloliquefaciens B1619 and the gamma-polyglutamic acid is 1:1.1: 1.8.
6. Use of the agricultural composition of any one of claims 1 to 5 for the control of fungal diseases.
7. The use according to claim 6, characterized in that said fungal diseases comprise tomato leaf mold.
8. The use according to claim 6, wherein said fungal disease comprises early blight of tomato.
9. The use according to claim 6, wherein the agricultural composition comprising a fungicide and gamma-polyglutamic acid is used for seed treatment.
10. Use according to claim 6, wherein the seed treatment comprises seed soaking or seed dressing.
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