CN114391545B - Pesticide composition containing bixafen and copper quinolinate and application thereof - Google Patents

Pesticide composition containing bixafen and copper quinolinate and application thereof Download PDF

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CN114391545B
CN114391545B CN202210124903.5A CN202210124903A CN114391545B CN 114391545 B CN114391545 B CN 114391545B CN 202210124903 A CN202210124903 A CN 202210124903A CN 114391545 B CN114391545 B CN 114391545B
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bixafen
pesticide composition
copper
quinoline
disease
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CN114391545A (en
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解维星
刘桂娟
杨志鹏
王飞菲
柴泽宇
范勇
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Qingdao Tengrunxiang Testing And Evaluation Co ltd
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Qingdao Tengrunxiang Testing And Evaluation Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A01N43/42—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings condensed with carbocyclic rings

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the field of pesticide bactericides and discloses a pesticide composition containing bixafen and copper quinoline; the active ingredient of the pesticide composition comprises active ingredient A bixafen and active ingredient B copper quinoline; the mass ratio of the bixafen to the copper quinoline is 10:1-1:20. The pesticide composition can be used for preventing and treating various diseases on crops, has good prevention and treatment effect, and has the characteristics of wide sterilization spectrum, low dosage, obvious synergistic effect, drug resistance delay and the like.

Description

Pesticide composition containing bixafen and copper quinolinate and application thereof
Technical Field
The invention relates to the field of pesticide bactericides, in particular to a pesticide composition containing bixafen and copper quinoline. The pesticide composition can enhance the pesticide effect, reduce the dosage, improve the quick acting property, prolong the lasting effect and delay the development of drug resistance.
Background
Cucurbitaceae (Cucurbitaceae) crops include common vegetables and fruits such as cucumber, white gourd, balsam pear, pumpkin, towel gourd, watermelon and melon. Pathogenic bacteria (Corynespora cassiicola) of the cucumber target spot disease are caused by infection of the corynespora polymorpha, the optimal temperature for growth of hyphae of the pathogenic bacteria is 28 ℃, the optimal temperature for spore production is about 30 ℃, the spore germination needs higher humidity, the relative humidity is more than 90% to germinate, and the germination rate in water drops is highest. The corynespora polycephala has the characteristics of good temperature and humidity, and the high temperature and the high humidity are favorable for the epidemic and spreading of the disease. The disease degree is increased by the condensation of leaves, insufficient illumination and day-night temperature difference, and the bacteria are propagated faster when the day-night temperature difference is larger. In addition, excessive use of the nitrogen fertilizer can cause overgrowth of plants or continuous cropping for many years, which is beneficial to morbidity. When the ventilation and light transmission conditions are poor, diseases are serious; the patients should pay attention to the cultivation after the autumn delay because of excessive rain and excessive cold in summer.
The bixafen (Bixafen) is a pyrazole amide fungicide developed by Bayer crop science company, has a wide bactericidal spectrum, acts on Succinic Dehydrogenase (SDH), one of hubs for connecting oxidative phosphorylation and electron transfer in bacteria, causes the disorder of tricarboxylic acid circulation (tricarboxylicacidcycle), blocks the energy metabolism of the bixafen, further inhibits the growth of pathogenic bacteria, and causes the death of the bixafen, thereby achieving the purpose of disease control, and the chemical name of the bixafen is N- [2- (3, 4-dichlorophenyl) -4-fluorophenyl ] -3- (difluoromethyl) -1-methylpyrazole-4-carboxamide.
The quinoline copper is a chelated organic copper broad-spectrum bactericide, and can prevent pathogenic bacteria from infecting crops by forming a compact protective film on the surface of the crops, and can also act inside the pathogenic bacteria to inhibit the activity and conduction of main conductors of the pathogenic bacteria, thereby killing the pathogenic bacteria. The quinclorac has the effect of preventing and controlling fungi and bacterial diseases, and is suitable for preventing and controlling various diseases on various crops such as fruit trees, vegetables, melons, vegetables, tobacco and the like.
Melon target spot disease has become an important global disease, and especially cucumber target spot disease has a great influence on the production and quality of cucumbers. Chemical control is still an important means for controlling the occurrence and development of target spot disease, but the pathogenic bacteria are easy to be changed and have strong destructiveness, so that the effective control of the disease is difficult. The reasonable chemical agent compounding has the positive characteristics of expanding the sterilization spectrum, improving the control effect, prolonging the application period, reducing the dosage, reducing the phytotoxicity, reducing the residue, delaying the drug resistance of harmful bacteria, delaying the occurrence of drug resistance and the like. On the basis of indoor screening and field experiments, the compound combination of bixafen and copper quinoline is screened out, and the synergistic effect is obvious.
Disclosure of Invention
Based on the above circumstances, the invention aims to provide a pesticide composition containing bixafen and copper quinoline and application thereof, and the pesticide composition has the characteristics of good control effect, wide sterilization spectrum, low dosage, obvious synergistic effect and the like.
In order to achieve the above purpose, a pesticide composition containing bixafen and copper quinoline, wherein the active ingredients of the pesticide composition comprise active ingredient A bixafen and active ingredient B copper quinoline;
further, the mass ratio of the bixafen to the copper quinoline is 10:1-1:30;
further, the mass ratio of the bixafen to the copper quinoline is 5:1-1:14;
further, the mass ratio of the bixafen to the copper quinolinate is 5:1, 2:1, 1:1, 2:3, 1:2, 1:3, 1:6, 1:8, 1:10 and 1:14;
Further, the mass ratio of the bixafen to the copper quinoline is 2:1-1:10;
Further, the mass ratio of the bixafen to the copper quinoline is 1:1-1:6;
Further, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 5 to 90 percent by weight based on 100 percent by weight of the total weight of the pesticide composition;
further, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 10 to 80 weight percent based on 100 weight percent of the total weight of the pesticide composition;
further, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 20 to 50 weight percent based on 100 weight percent of the total weight of the pesticide composition;
in an embodiment, the sum of the contents of bixafen and copper quinoline in the pesticide composition is 20 to 50wt%, for example, the sum of the contents of bixafen and copper quinoline is 20wt%, 25wt%, 35wt%, 40wt% and 48wt%;
further, the formulation of the pesticide composition can be any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspending agent, wettable powder or water dispersible granule;
Further, the formulation of the pesticide composition can be any one of suspending agent and water dispersible granule;
Further, the pesticide composition is prepared by mixing the composition with a proper surfactant, water or an organic solvent according to a certain proportion, using glass beads, crushing by a sand mill to obtain crushed slurry, adding a thickening agent, a preservative, a defoaming agent, an antifreezing agent, deionized water and the like into the crushed slurry, and mixing the crushed slurry, wherein the suspending agent is divided into an aqueous suspending agent and an oil suspending agent, and has the advantages of small particle size, high biological activity, no dust flying problem and non-inflammability and explosiveness;
Further, the suspending agent comprises 1-20% of bixafen, 10-50% of copper quinoline, 1-20% of wetting dispersant, 0.1-2% of thickener, 1-10% of antifreezing agent, 0.01-3% of preservative, 0.1-5% of defoamer and deionized water which are complemented to 100%;
further, the pesticide composition is prepared from water dispersible granules, wherein the water dispersible granules are prepared by mixing the composition with a proper surfactant and other functional additives according to a certain proportion, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening;
Further, the wetting agent dispersant is selected from one or more of lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol ethoxylate methyl ether condensate sulfate, fatty amine ethoxylate, glycerin fatty acid ester ethoxylate, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and/or
The thickener is one or more selected from xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and/or
The antifreezing agent is one or more selected from alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and/or
The preservative is one or more selected from propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, pinocembrane and 1, 2-benzisothiazolin-3-one; and/or
The defoamer is one or more selected from C 10-C20 saturated fatty acid compounds, silicone oil, silicone compounds and C 8-C10 fatty alcohol; and/or
The invention also provides the application of the pesticide composition containing bixafen and copper quinolinate in preventing and controlling crop diseases,
Further, the crop diseases are cucurbitaceae crop diseases, and specifically comprise melon downy mildew, melon target spot disease, melon fusarium wilt, melon powdery mildew, melon epidemic disease, melon anthracnose, melon scab, melon virus disease, melon sclerotinia, melon bacterial angular leaf spot disease, melon gummy stem blight, melon bacterial fruit spot disease, melon gray mold, melon silver leaf disease and melon root knot nematode;
Further, the cucurbitaceae crop disease is any one of melon downy mildew, melon target spot and melon powdery mildew;
Further, the pathogenic bacteria of the melon target spot disease is (Corynespora cassiicola);
Further, the cucurbitaceae crops comprise cucumbers and melons;
Further, the dosage of the effective components of the pesticide composition for preventing and treating the cucumber target spot disease is 200-500 g.ai/hm 2;
further, the pesticidal composition is applied to a disease to be controlled or a growth medium thereof in an effective dose.
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
(1) The bactericidal activity is increased and the application range is enlarged by compounding the bixafen and the copper quinoline;
(2) The pesticide is safe and environment-friendly, has wide development prospect, reduces the use amount of the pesticide and reduces the agricultural cost;
(3) Has the advantages of super high efficiency, broad bactericidal spectrum, and can delay the generation of drug resistance of harmful bacteria and prolong the duration of the drug.
Detailed Description
The present invention will be described in more detail with reference to the following examples, but the present invention can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein.
Examples reference pesticide indoor biometric test guidelines, part 2: test plate method for inhibiting growth of pathogenic fungus hypha, NY/T1156.2-2006; part 6: the combined effect of the compounding was determined for NY/T1156.6-2006.
Experimental materials: culturing pathogenic fungi for test on a proper culture medium for standby;
experimental instrument equipment: electronic balance (sensing 0.01 mg), artificial climate box, pipette, culture dish, puncher, inoculator, etc.
Example 1
Determination of toxicity of bixafen and copper quinoline on cucumber target spot
Test pathogenic bacteria: cucumber target spot (Corynespora cassiicola), supplied by the halier pharmaceutical industry group development center.
Test agent: 98% of bixafen is provided by the research and development center of the Hailier pharmaceutical industry group, and 33.5% of the quinoline copper suspending agent is provided by Shandong Hailier chemical industry Co.
And (3) preparation of a medicament: dissolving bixafen in acetone, and diluting with 0.1% Tween 80 water solution; 33.5% copper quinolinate suspension was diluted with sterile water. The mother solution of single agent is prepared respectively, 10 groups of proportions are designed according to the mixing purpose and the medicament activity, and 5 series of mass concentrations of each single agent and each group of proportion mixture are prepared according to an equal ratio method.
Under the aseptic operation condition, adding 45ml of pre-melted sterilization culture medium into an aseptic conical flask according to test treatment, sequentially sucking 5ml of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, fully shaking uniformly, and then pouring into 3 culture dishes in equal quantity to prepare a medicine-containing flat plate with corresponding concentration. The test was run with no drug treatment as a blank, and 3 replicates per treatment.
The cultured pathogenic bacteria are inoculated in the center of a medicine-containing flat plate by an inoculator, the mycelium surface faces upwards, 1 bacterial cake is placed in each culture dish, the dish cover is covered, and the culture dish is placed in a (26+/-0.5 ℃) incubator for culture.
Data statistics and analysis:
The growth of pathogenic hyphae was investigated according to the growth of bacteria in a blank culture dish. Colony diameter was measured in millimeters (mm) using calipers. The diameter of each colony was measured vertically by the cross-over method and the average value was taken.
According to the investigation result, the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested is calculated, wherein the unit is the calculated result of percentage (%) and two positions after decimal point are reserved.
D＝D1-D2
Wherein:
D-colony growth diameter;
D 1 —colony diameter;
D 2 -diameter of the fungus cake.
Wherein:
i, hypha growth inhibition rate;
D 0 —a control colony growth diameter;
D t —agent treated colony growth diameter.
And (5) carrying out regression analysis according to the concentration log value of each medicament and the corresponding hypha growth inhibition rate probability value. The EC 50 value and 95% confidence limit and correlation coefficient r of the virulence regression line were determined.
Grand cloud Pei method: evaluating the synergistic effect of the mixed medicaments according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient CTC is more than or equal to 120 and shows the synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
Co-toxicity coefficient (CTC value) of the blend was calculated:
Wherein:
ati—actual measured virulence index of the mixture;
S-EC 50 of standard bactericide in milligrams per liter (mg/L);
M-EC 50 of the mixture in milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A A agent toxicity index;
the percentage content of the P A -A medicament in the mixture is expressed as percentage (%);
TI B -agent toxicity index;
the percentage of the P B -B medicament in the mixture is expressed as percentage (%).
Wherein:
Ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
Results and analysis:
As can be seen from table 1: the single bixafen and the copper quinoline compound preparation have good bactericidal activity on the cucumber target spot, the EC 50 value of the single bixafen is 8.292mg/L, and the EC 50 value of the single copper quinoline is 9.798mg/L. The mass ratio of the bixafen to the copper quinoline is within the range of 2:1-1:10, wherein EC 50 is 4.980mg/L when the mass ratio of the bixafen to the copper quinoline is 1:6, the co-toxicity coefficient is 191.771, and the synergistic effect is shown.
TABLE 1 results of indoor bioassay of Bifiduciary amine, copper quinolinate, and their compound formulations on cucumber target spot pathogens
Specific preparation examples:
Preparation example 1:20wt% bixafen-quinoline copper suspension (1:3)
The formula comprises the following components: 5% of bixafen, 15% of copper quinoline, 3.5% of sodium dodecyl sulfate, 2% of aryl phenol ether phosphate, 3.3% of alkylphenol formaldehyde resin polyoxyethylene ether, 0.7% of carboxymethyl cellulose, 6% of ethylene glycol, 0.03% of benzisothiazolinone, 0.6% of organosilicon defoamer and deionized water, and the balance of the components;
The preparation method comprises the following steps: according to the formula proportion, active ingredients of bixafen, copper quinoline, a surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, a suspending agent product is obtained through homogenizing and filtering.
Preparation example 2:25wt% bixafen-quinoline copper suspension (1:5)
The formula comprises the following components: 5% bixafen, 20% of copper quinoline, 5% of sodium dodecyl sulfate, 1.5% of aryl phenol ether phosphate, 4% of alkylphenol formaldehyde resin polyoxyethylene ether, 0.7% of carboxymethyl cellulose, 0.03% of methyl parahydroxybenzoate, 5% of ethylene glycol, 0.5% of organosilicon defoamer and deionized water, wherein the balance is the balance;
The preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 3:35wt% bixafen-quinoline copper suspension (1:6)
The formula comprises the following components: 5% bixafen, 30% copper quinoline, 1% naphthalene sulfonate, 0.5% lignin sulfonate, 1% polycarboxylate, 0.7% carboxymethyl cellulose, 5% glycol, 0.03% methyl parahydroxybenzoate, 0.6% organosilicon defoamer and deionized water are added for the rest;
The preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 4:40wt% bixafen-quinoline copper water dispersible granule (1:4)
The formula comprises the following components: 8% bixafen, 32% quinoline copper, 2% lignosulfonate, 8% sodium dodecyl benzene sulfonate, 4.5% aluminum chloride and white carbon black are used for the balance;
the preparation method comprises the following steps: according to the formula proportion of the embodiment, active ingredients bixafen and copper quinoline are added into a carrier, a surfactant and other functional auxiliary agents are added into the carrier, the carrier is mixed, 10-25% of water is added after air current crushing, and then the water dispersible granule product is prepared through kneading, granulating, drying and screening.
Preparation example 5:48wt% bixafen-quinoline copper water dispersible granule (1:5)
The formula comprises the following components: 8% bixafen, 40% quinoline copper, 5% lignin sulfonate, 7.5% sodium dodecyl benzene sulfonate, 4% aluminum chloride and white carbon black are used for the balance;
The preparation method comprises the following steps: the same as in preparation example 4.
Example 2
Field test for preventing and treating cucumber target spot disease
Test crop: cucumber, the variety is "Toyota No. 16";
test object: target spot (Corynespora cassiicola);
Test compounding of the drug: 20% of bixafen-quinoline copper suspension (1:3), 40% of bixafen-quinoline copper water dispersible granule, 35% of bixafen-quinoline copper suspension (1:6), 35% of fluopyram-quinoline copper suspension (1:6), 32% of fluopyram-quinoline copper suspension (1:3), 40% of fluopyram-quinoline copper water dispersible granule (1:5), 30% of fluopyram-quinoline copper water dispersible granule (1:3) and 30% of fluoxastrobin-quinoline copper suspension (1:1);
control single dose: 40% bixafen suspending agent and 33.5% quinoline copper suspending agent;
Drug treatment and dosage:
TABLE 2 test design of reagents for test
The test is carried out on village vegetable bases in Banqi Zhenyan in Minhou county of Fuzhou, on open field cultivation, in fertilizer and water management, in soil fertility and the like. The plant row spacing is 30 multiplied by 60cm, the growth vigor is good, and no bactericide is sprayed from the plant field planting to the first application period. The generation of the target spot disease of the cucumber in the past year of the test field is serious, so that the cucumber leaves are provided with massive spot, and the whole plant leaves die when serious. The conditions of soil, cultivation, water and fertilizer management and the like in each test area are consistent.
Test treatment: each treatment adopts random group arrangement, buffer lines are arranged between adjacent cells, the treatment is repeated for 4 times, and the cell area is 30m 2.
The application method comprises the following steps: the 3WBD-16 knapsack electric sprayer is used for adding water to spray according to the application dosage, so as to achieve uniform and comprehensive spraying, and the front and back surfaces of the leaves of the cucumber plant to be tested are uniformly hung with fogdrops. The average liquid medicine consumption per mu is 80L.
Time of application:
Two doses were given together on days 7, 15 and 22 of 2017. At the beginning of the test, plant leaves can see sporadic target spot disease spots. The day maximum air temperature during the test was 39℃and the minimum air temperature was 25 ℃. The total rainfall is 188mm after rain day days of the whole test.
Two doses were co-administered on days 6, 17 and 24 of 2018. At the beginning of the test, plant leaves can see sporadic target spot disease spots. The day maximum air temperature during the test was 34℃and the minimum air temperature was 23 ℃. The whole test course is rain day days, and the total rainfall is 104mm.
The investigation method comprises the following steps: only individual leaves at the beginning of the trial were visible for the target plaque, so the disease cardinality was not investigated.
Investigation time:
Investigation of cucumber target spot disease conditions when the conditions of 2017 8-5 blank control cells are stable;
and (5) investigating the disease condition of the cucumber target spot disease when the disease condition of a blank control district is stable in 7-8 days of 2018.
Reference is made to the national standard "pesticide field efficacy test criterion (one)", part 26: the bactericide can be used for preventing and treating cucumber downy mildew (GB/T17980.26-2000), and carrying out grading investigation on occurrence of cucumber target spot.
The first time of pesticide application in the growing period of cucumber is in the full period of flowering and fruiting, and 10-15 leaves are grown.
5 Points are randomly selected from each test district for investigation, the disease conditions of the target spot disease of all leaves of 2 cucumbers are checked at each point, and the total leaf number and the leaf number of each stage of disease are recorded.
The lesion classification criteria were as follows:
Level 0: no disease spots;
Stage 1: the area of the disease spots accounts for less than 5% of the whole leaf area;
3 stages: the area of the lesion accounts for 6-10% of the whole leaf area;
5 stages: the area of the lesion accounts for 11% -20% of the whole leaf area;
7 stages: the area of the lesion accounts for 21% -50% of the whole leaf area;
Stage 9: the area of the disease spots accounts for more than 50% of the whole leaf area.
The drug effect is calculated according to the following formula:
(1) Disease index = { Σ (leaf number of each stage relative grade value)/(total leaf number of investigation ×9) } 100
(2) Control effect (no basal before administration)% = (disease index of blank control area-disease index of treatment area)/disease index of blank control area is 100
2017 Test results
Test results of each treatment agent for preventing and treating cucumber target spot disease
TABLE 3 results of field trials of different reagents for cucumber target leaf spot
The results show that: the 35% bixafen-quinoline copper suspending agent (1:6) of the test agent has good control effect on cucumber target spot, the control effect is 85.84% when the application dose is 300 g.ai/hm 2, and the control effects of the 20% bixafen-quinoline copper suspending agent (1:3) and the 40% bixafen-quinoline copper water dispersible granule are relatively poor under the same application dose, wherein the control effects are 81.91% and 81.70% respectively.
The treatments did not produce phytotoxicity to cucumber plants, nor was the test agent found to have other effects on the test plants during the observed period of application.
2018 Test results
Test results of each treatment agent for preventing and treating cucumber target spot disease
TABLE 4 results of field trials of different reagents for cucumber target leaf spot
The results show that: the tested medicament 40% bixafen-quinclorac water dispersible granule (1:4) and 35% bixafen-quinclorac suspending agent (1:6) have good control effect on cucumber target spot, and the control effect of 300 g.ai/hm 2 of the applied medicament is 89.34% and 90.70% respectively.
The treatments of the test agents do not have phytotoxicity on cucumber plants, and other influences of the test agents on test plants are not found in the observation period of the application of the agents.
Through indoor toxicity measurement and field experiments, the pesticide composition compounded by the bixafen and the copper quinoline disclosed by the invention has a good control effect on crop diseases, especially cucurbitaceae crop diseases, especially cucumber target spot. The pesticide composition or the preparation prepared by the combination has remarkable prevention effect, and is superior to a single dose in the aspects of delaying the generation of drug resistance and prolonging the drug retention. In addition, no phytotoxicity of the compound medicament to crops is found in the test, which proves that the production cost and the use cost can be reduced and the pesticide composition or the pesticide preparation is safe to crops under the condition that the sterilization synergy of the obtained pesticide composition or the pesticide preparation is improved.
While the invention has been described in detail in terms of the general description and the specific embodiments, it will be apparent to those skilled in the art that various modifications and improvements can be made thereto without departing from the spirit of the invention.

Claims (10)

1. The pesticide composition containing bixafen and copper quinoline is characterized in that: the pesticide composition comprises active ingredients A bixafen and active ingredients B copper quinoline; the mass ratio of the bixafen to the copper quinoline is 2:1-1:10.
2. A pesticidal composition according to claim 1, characterized in that: the mass ratio of the bixafen to the copper quinoline is 1:1-1:6.
3. A pesticidal composition according to claim 1, characterized in that: the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 20-50wt% based on 100wt% of the total weight of the pesticide composition.
4. A pesticidal composition according to claim 1, characterized in that: the pesticide composition can be any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspending agent, wettable powder or water dispersible granule.
5. A pesticidal composition according to claim 4, characterized in that: the pesticide composition is in the form of any one of a suspending agent and a water dispersible granule.
6. Use of the pesticidal composition according to any one of claims 1 to 5 for controlling crop diseases.
7. The use according to claim 6, wherein: the crop disease is cucurbitaceae crop disease, and the cucurbitaceae crop disease is melon target spot disease.
8. The use according to claim 7, wherein: the pathogenic bacteria of the melon target spot disease is Corynespora cassiicola.
9. The use according to claim 6, wherein: the dosage of the effective components of the pesticide composition for preventing and treating crop diseases is 200-500 g.ai/hm 2.
10. The use according to claim 9, characterized in that: the pesticide composition is applied to diseases or growth media thereof to be controlled in an effective dose.
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