CN117481126A - Pesticide composition and application thereof - Google Patents

Pesticide composition and application thereof Download PDF

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Publication number
CN117481126A
CN117481126A CN202311444449.2A CN202311444449A CN117481126A CN 117481126 A CN117481126 A CN 117481126A CN 202311444449 A CN202311444449 A CN 202311444449A CN 117481126 A CN117481126 A CN 117481126A
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Prior art keywords
active ingredient
pesticide composition
thiophanate
methyl
carbendazim
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Inventor
葛尧伦
葛晓甜
刘桂娟
刘金玲
杨志鹏
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Qingdao Hailier Biotechnology Co ltd
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Qingdao Hailier Biotechnology Co ltd
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Priority to CN202311444449.2A priority Critical patent/CN117481126A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a pesticide composition and application thereof, wherein the pesticide composition comprises an active ingredient A and an active ingredient B, the active ingredient A is cyclopurifiham, the active ingredient B is selected from any one of carbendazim and thiophanate-methyl, and the mass ratio of the active ingredient A to the active ingredient B is 1:42-50:1. The pesticide composition has good control effect on fusarium fungi, and particularly has remarkable control effect on melon fusarium wilt and wheat scab.

Description

Pesticide composition and application thereof
Technical Field
The invention belongs to the technical field of pesticides, and discloses a pesticide composition and application thereof.
Background
Thiophanate methyl, also called thiophanate methyl, is a broad-spectrum internal-sucking benzimidazole bactericide developed by the company Cao in Japan, has the functions of sucking, preventing and treating, and can interfere the formation of a fungus spindle body after thiophanate methyl is absorbed by plants, so that germ sprouting bud tubes are abnormal, and the bactericidal effect is achieved. The composition can prevent and treat scab of fruit trees and flowers and anthracnose, and has good prevention and treatment effects on corn size spot.
Carbendazim is a broad-spectrum benzimidazole bactericide, has good control effect on fungal diseases such as scab, black spot and anthracnose of crops, and has two main mechanisms of action: one is to hinder the production of nucleosides such that nucleic acid synthesis is disturbed; the other is to interfere with spindle formation during mitosis by inhibiting tubulin accumulation, thereby affecting cell division.
In the actual process of agricultural production, chemical control is still the current main means of controlling plant pathogenic bacteria. Studies have shown that, due to the frequent use of single agents, the control of diseases caused by plant pathogens by some commonly used agents has been significantly reduced, and pathogens have developed resistance to most single agents. Therefore, the germicides with different action mechanisms are mixed for use to inhibit the generation of pathogen resistance, thereby meeting the production requirements. The inventor conducts a great deal of experimental study to find that the compound formulation of the Cyclobrifluham and any one of carbendazim or thiophanate-methyl shows good control effect on plant diseases caused by fusarium fungi within a certain range, reduces the dosage, lowers the production cost and is safe to the environment.
Disclosure of Invention
Based on the purposes and the contents, the invention provides a pesticide composition and application thereof, and the pesticide composition is used for preventing and controlling plant diseases caused by fusarium fungi, has remarkable control effect especially on wheat scab and watermelon fusarium wilt, effectively slows down the invasion of pathogenic bacteria to plants, and reduces the harm and increases the yield.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is cycloprifluram, and the active ingredient B is any one of carbendazim and thiophanate-methyl.
Further, the mass ratio of the active ingredient A to the active ingredient B is 1:42-50:1;
further, the active ingredient B is carbendazim, and the mass ratio of the active ingredient Cyclobutrifluram to the carbendazim is 1:42-42:1;
further, the mass ratio of the active ingredients of the cyclob rifluram to the carbendazim is 1:20-25:1;
further, the active ingredient B is thiophanate-methyl, and the mass ratio of the Cyclobutrifluram to the thiophanate-methyl is 1:32-32:1;
further, the mass ratio of the active ingredients of the cyclob rifluram to the thiophanate-methyl is 1:25-32:1;
further, the weight ratio of the active ingredient A to the active ingredient B accounts for 0.5-80% of the total weight of the pesticide composition based on 100 wt%;
further, the pesticide composition comprises other auxiliary components besides the active components, wherein the auxiliary components are selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.
Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium succinate, alpha olefin sulfonate, alkylphenol ethoxylate, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrating agent F; and/or
Further, the dispersing agent is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and/or
Further, the emulsifier is one or more selected from calcium dodecyl benzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and/or
Further, the thickener is one or more selected from xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and/or
Further, the disintegrating agent is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and/or
Further, the antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and/or
Further, the defoamer is selected from C 10 -C 20 One or more of saturated fatty acid compounds, silicone oil, silicone compounds and C8-C10 fatty alcohols; and/or
Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives and water; and/or
Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium parahydroxybenzoate, methyl parahydroxybenzoate, pinocembrane and 1, 2-benzisothiazolin-3-one; and/or
Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomite, bentonite, attapulgite, white carbon black, talcum powder, montmorillonite and starch; and/or
Further, the synergist is selected from synergistic phosphorus and synergistic ether; and/or
Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.
Further, the pesticide composition can be prepared into a preparation formulation comprising suspending agents, aqueous emulsion, microemulsion, emulsifiable concentrate, dispersible oil suspending agent, soluble agent, water dispersible granule, wettable powder, granule, seed treatment suspending agent or seed treatment microcapsule suspending agent;
furthermore, the preparation is in the form of suspending agent, wettable powder or water dispersible granule.
The invention also discloses the application of the pesticide composition in preventing and controlling plant diseases.
Further, the invention reasonably compounds the Cyclobrifluram with carbendazim or thiophanate-methyl, and can be used for preventing and treating any plant diseases caused by fusarium fungi;
further, the fusarium fungi are fusarium oxysporum, fusarium putrescens, fusarium moniliforme, fusarium graminearum, fusarium pseudograminearum, fusarium equisetum, fusarium xanthophyllum, fusarium avenae and fusarium layering;
furthermore, the pesticide composition can prevent or control plant diseases caused by infection of one fusarium or mixed infection of multiple fusarium;
further, the plant diseases are wheat scab, wheat stem-based rot, soybean root rot, rice bakanae disease, melon wilt, pseudo-ginseng root rot, corn stem-based rot, tobacco root rot, banana wilt and astragalus root rot;
further, the plant disease is wheat scab and melon fusarium wilt;
the invention has the beneficial effects that: the invention reasonably compounds different active ingredients, effectively controls plant diseases caused by fusarium fungi, has obvious synergism especially for wheat scab and watermelon fusarium wilt, improves crop yield while slowing down the generation of pathogenic bacteria drug resistance, and is safe to target crops and environment-friendly.
Detailed Description
In order to make the technical solution and advantages of the present invention more apparent, the present invention is further illustrated by the following preparation examples and examples of specific formulations, but the scope of the present invention should not be limited by the specific embodiments herein.
Preparation example:
preparation example 1:24% Cyclokutrifluham carbendazim suspension (1:1)
The formula comprises the following components: 12% of cyclob utrifluram, 12% of carbendazim, 2% of sodium dodecyl sulfate, 1% of naphthalene sulfonate formaldehyde condensate, 4% of styrol polyoxyethylene ether phosphate, 0.25% of xanthan gum, 1% of magnesium aluminum silicate, 5% of ethylene glycol, 0.01% of benzisothiazolinone potassium, 0.5% of silicone oil and deionized water, and the balance of the composition;
the preparation method comprises the following steps: according to the formula proportion, the active ingredients, the surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, the suspending agent product is obtained through homogenizing and filtering.
Preparation example 2:40% Cyclobinifluram carbendazim water dispersible granule (3:1)
The formula comprises the following components: 30% of Cyclobutrifluram, 10% of carbendazim, 8% of lignosulfonate, 5% of naphthalene sulfonate formaldehyde condensate, 2% of sodium dodecyl sulfate, 5% of white carbon black, 25% of starch and the balance of kaolin;
the preparation method comprises the following steps: adding active ingredients into a carrier according to the formula proportion, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain a water dispersible granule product; or spraying water, granulating, drying, and sieving to obtain the final product.
Preparation example 3:45% Cyclokutrifluham carbendazim wettable powder (1:8)
The formula comprises the following components: 5% of Cyclobutrifluram, 40% of carbendazim, 8% of sodium lignin sulfonate, 5% of dispersant NNO, 2% of sodium dodecyl sulfate and the balance of kaolin.
The preparation method comprises the following steps: according to the formula proportion, the active ingredients, the dispersing agent, the wetting agent and the filler are mixed, uniformly stirred in a stirring kettle, and crushed and mixed uniformly for many times by a jet mill, thus the wettable powder of the composition of the invention can be prepared.
Preparation example 4:30% Cyclobutrifluram thiophanate-methyl suspension (1:1)
The formula comprises the following components: 15% of Cyclobutrifluram, 15% of thiophanate methyl, 2% of fatty alcohol polyoxyethylene ether, 2% of alkylaryl polyoxyethylene ether polyoxypropylene ether, 3% of styrol polyoxyethylene ether sulfate ammonium salt, 2% of polycarboxylic acid sodium salt, 1.5% of magnesium aluminum silicate, 0.1% of carboxyethyl cellulose, 1% of sodium benzoate, 5% of ethylene glycol, 0.5% of silicone oil and deionized water, and the balance of the components are filled;
the preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 5:42% Cyclokutrifluram thiophanate-methyl wettable powder (1:5)
The formula comprises the following components: 7% of Cyclobutrifluram, 35% of thiophanate-methyl, 6% of sodium polycarboxylate, 6% of dispersant NNO, 3% of succinate sulfonate and the balance of kaolin.
The preparation method comprises the following steps: the same as in preparation example 3.
Preparation example 6:39% Cyclobinifluram thiophanate-methyl water dispersible granule (12:1)
The formula comprises the following components: 36% of Cyclobutrifluram, 3% of thiophanate-methyl, 8% of naphthalene sulfonate formaldehyde condensate, 2% of sodium salt of polycarboxylic acid, 2% of sodium dodecyl sulfate, 10% of ammonium sulfate, 15% of starch and kaolin are used for the balance.
The preparation method comprises the following steps: the same as in preparation example 2.
Example 1: indoor activity determination test for watermelon fusarium wilt by compounding Cyclobutrifluram with carbendazim or thiophanate-methyl
The test method comprises the following steps: a hypha growth rate method;
test target: watermelon fusarium wilt (Fusarium oxysporium f.sp.niveum);
test agent: 98% of carbendazim technical, 90% of Cyclobutrifluram technical and 95% of thiophanate-methyl technical;
and (3) preparation of a medicament: dissolving the above raw materials with appropriate solvent, and diluting with 0.1% Tween 80 water solution. Each agent was formulated in equal ratio to a corresponding mass concentration of drug-containing plate, and each treatment was repeated 4 times with PDA medium plates without agent added as controls.
Inoculating and culturing: inoculating pre-cultured watermelon fusarium wilt bacteria on each agent flat plate by using a sterilized puncher, culturing in a constant temperature incubator at 25 ℃, measuring the diameter of each treated colony by using a crisscross method when the diameter of the control colony reaches about 2/3 of the diameter of the culture dish, and calculating the hypha growth inhibition rate.
Data processing and calculation:
statistics and analysis: according to the investigation result, the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested is calculated, wherein the unit is the calculated result of percentage (%) and two positions after decimal point are reserved.
D＝D 1 -D 2
Wherein:
d-colony growth diameter;
D 1 colony diameter;
D 2 -diameter of the bacterial cake.
Wherein:
i, hypha growth inhibition rate;
D 0 -the control colony increased in diameter;
D T -the agent-treated colonies increased in diameter.
Analysis by DPS statistical analysis system to obtain EC 50 And evaluating the activity of the test agent on the biological test material.
Grand cloud Pei method: evaluating the synergistic effect of the mixed medicaments according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient CTC is more than or equal to 120 and shows the synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
Co-toxicity coefficient (CTC value) of the blend was calculated:
wherein:
ati—actual measured virulence index of the mixture;
S-EC of Standard Agents 50 Milligrams per liter (mg/L);
M-EC of mixture 50 Units are milligrams per liter (mg/L).
TTI＝TI A ×P A +TI B ×P B
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A -a medicament virulence index;
P A -the percentage of agent a in the mix, in percent (%);
TI B -B agent virulence index;
P B the percentage of the B medicament in the mixture is expressed as percentage (%).
Wherein:
ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The test results are shown in the following table:
TABLE 1 results of indoor Activity determination test of Cyclobutrifluram and carbendazim for watermelon fusarium wilt
TABLE 2 results of indoor Activity determination test of Cyclobutrifluram and thiophanate-methyl against watermelon fusarium wilt
As shown in tables 1 and 2, the Cyclobutrifluram and carbendazim or thiophanate-methyl are compounded in a reasonable proportion, and the composition has good control effect on watermelon fusarium wilt. In Table 1, when the mass ratio of Cyclobrifluham to carbendazim is 50:1, the co-toxicity coefficient of Cyclobrifluham to watermelon fusarium wilt is less than 120, and the added effect is shown; in the mass ratio range of 1:42-42:1, the two compounds show better inhibition effect on the growth of the watermelon fusarium wilt hyphae, and the co-toxicity coefficient is more than 120, thus showing synergistic effect. In Table 2, when the mass ratio of Cyclobrifluham to thiophanate-methyl is 1:30, the co-toxicity coefficient to watermelon fusarium wilt is 119.532, which shows additive effect; and the mass ratio of the two is in the range of 1:25-45:1, the co-toxicity coefficient of the two to watermelon fusarium wilt is more than 120, and the synergistic effect is shown.
Example 2: cyclobutrifluram and carbendazim or thiophanate-methyl compounded indoor activity determination test for wheat scab
The test is based on: test reference NY/T1156.2-2006 agricultural industry standard "pesticide indoor bioassay test criteria section 2 of the people's republic of China: test plate method for inhibiting growth of pathogenic bacterial hyphae.
Test materials: the pathogen tested was wheat scab pathogen (Fusarium graminearum).
Test medium: potato Dextrose Agar (PDA).
Test agent: 90% of Cyclobutrifluram raw medicine, 95% of thiophanate-methyl raw medicine and 98% of carbendazim raw medicine.
And (3) preparation of a medicament: dissolving the above raw materials with proper solvent, diluting with 0.1% Tween 80 water solution, and setting 5 series of mass concentration gradients for each medicament treatment.
And (3) medicament treatment: under aseptic condition, adding pre-melted sterilizing culture medium into aseptic conical flask according to test treatment, sequentially quantitatively sucking medicinal liquid from low concentration to high concentration, respectively adding into the conical flask, and shaking thoroughly. Then the mixture is poured into a culture dish in equal quantity to prepare a medicine-containing flat plate with corresponding concentration. The trial set up the treatment without the agent as a blank, each treatment was repeated 4 times.
Indoor toxicity measurement: taking bacterial cakes along the edges of bacterial colonies by using a 0.6cm puncher under aseptic conditions, then placing the bacterial cakes on a prepared medicine-containing flat plate lightly, culturing in a constant-temperature incubator at 25 ℃, measuring the diameter of each treated bacterial colony by using a crisscross method when the diameter of a control bacterial colony reaches about 2/3 of the diameter of a culture dish, and calculating the hypha growth inhibition rate.
Data processing and calculation:
statistics and analysis: according to the investigation result, the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested is calculated, wherein the unit is the calculated result of percentage (%) and two positions after decimal point are reserved.
D＝D 1 -D 2
Wherein:
d-colony growth diameter;
D 1 colony diameter;
D 2 -diameter of the bacterial cake.
Wherein:
i, hypha growth inhibition rate;
D 0 blank controlColony growth diameter;
D T -the agent-treated colonies increased in diameter.
Analysis by DPS statistical analysis system to obtain EC 50 And evaluating the activity of the test agent on the biological test material.
Grand cloud Pei method: evaluating the synergistic effect of the mixed medicaments according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient CTC is more than or equal to 120 and shows the synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
Co-toxicity coefficient (CTC value) of the blend was calculated:
wherein:
ati—actual measured virulence index of the mixture;
S-EC of Standard Agents 50 Milligrams per liter (mg/L);
M-EC of mixture 50 Units are milligrams per liter (mg/L).
TTI＝TI A ×P A +TI B ×P B
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A -a medicament virulence index;
P A -the percentage of agent a in the mix, in percent (%);
TI B -B agent virulence index;
P B the percentage of the B medicament in the mixture is expressed as percentage (%).
Wherein:
ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The test results are shown in the following table:
TABLE 3 results of test for indoor Activity determination of Cyclobutrifluram and carbendazim on wheat scab
TABLE 4 test results of indoor Activity determination of Cyclobutrifluram and thiophanate-methyl on wheat scab
As shown in tables 3 and 4, the cyclob rifluram and carbendazim or thiophanate-methyl are compounded in a reasonable proportion, so that the compound has a good inhibition effect on the growth of wheat scab hyphae. In Table 3, when the mass ratio of Cycloborifluram to carbendazim is 1:25 and 30:1, the co-toxicity coefficient of Cycloborifluram to wheat scab is less than 120, and the added effect is shown; in the mass ratio range of 1:20-25:1, the two compounds show good inhibition effect on the growth of wheat scab hypha, and the co-toxicity coefficient is more than 120, which shows synergistic effect. In Table 4, when the mass ratio of Cycloborifluram to thiophanate-methyl is 48:1, the co-toxicity coefficient to wheat scab is 109.108, which shows additive effect; and the total toxicity coefficient of the two is greater than 120 in the mass ratio range of 1:32-32:1, and the synergistic effect is shown.
Example 4: pesticide effect test for controlling watermelon fusarium wilt in field
Test site: the water and fertilizer management of each treatment area is consistent in vegetable greenhouses in the Shandong province of longevity.
Test target: watermelon fusarium wilt;
test crop: watermelon (jing xin No. 1).
Experiments were carried out on a Tian Mazhen watermelon plant in the Shandong Shouguang city, and planting was carried out on 28 days, 3 months and 4 months and 7 days of 2022.
The test adopts random block arrangement, 20m per cell 2 Each treatment was repeated 4 times. The pesticide is applied at the early stage of watermelon fusarium wilt, the pesticide is applied by diluting each treatment pesticide with water in a root irrigation mode, and the growth vigor of the selected watermelon seedlings is basically consistent. The administration dates were 2022, 4, 14, 2022, 4, 24, and 10 days apart, for a total of 2 administrations, with the specific administration doses shown in the table below.
The investigation method comprises the following steps: trial disease investigation was performed 10d after the last administration. The total plant number and the disease number of the fusarium wilt of the watermelons in each district are investigated, and the disease rate and the control effect are calculated by the following formula.
The results of the field efficacy test are shown in the following table:
table 5 results of field test of efficacy against watermelon fusarium wilt
The results in Table 5 show that the results of the field efficacy cell test show that 40% of the Cycloxafiluram and carbendazim water dispersible granule (3:1), 30% of the Cycloxafiluram and thiophanate-methyl suspending agent (1:1), 24% of the Cycloxafiluram and carbendazim suspending agent (1:1) and 42% of the Cycloxafiluram and carbendazim water dispersible granule (3:1)
The control effects of the wettable powder (1:5) of the Cyclobutrifluram and thiophanate-methyl on watermelon fusarium wilt are 89.95%, 93.45%, 88.48% and 86.92% respectively.
Example 5: prevention and treatment of wheat scab in field
Test site: the village wheat fields of the Yangzhen Hebei village are suitable for the Yizhou district of the city, the test land is flat, and the fertility is medium. The previous crop in the test field is rice. All test plots field management was consistent with local agricultural production practices, and no other germicides were used during the test.
Test target: wheat scab.
Test crop: wheat (zhenmai No. 9).
And (3) test design: the test sets 8 treatments in total, 20m per cell area 2 Each treatment was repeated 4 times with a random block arrangement.
Time of application and investigation: the test is carried out for 1 time at the early stage of wheat flowering, and the pesticide is applied by adopting a Gongnong-16 knapsack sprayer during the pesticide application. Test investigation wheat scab control effect investigation is carried out in the period of wheat milk maturity, 5 points are sampled on the diagonal line of each district during investigation, 100 ears are investigated at each point, and the number of the ears and the grade of the ears are investigated.
Investigation time: the test is carried out on the day of 5 months and 8 days, the test is carried out in the middle period, a diagonal 5-point sampling investigation method is adopted for each cell, 100 ears are investigated for each point, the number of the sick ears and the sick ear level are investigated, and the number of the sick ears and the total ears are recorded.
Yield investigation: test samples were taken during the wheat harvest period and 667m per cell was measured after 3d of air-drying 2 Actual yield.
The classification method of the disease spike grade comprises the following steps:
level 0: the whole spike is free from diseases;
stage 1: the area of the dried spike accounts for less than 1/4 of the area of the whole spike;
3 stages: the area of the dried spike accounts for 1/4 to 1/2 of the area of the whole spike;
5 stages: the area of the dried spike accounts for 1/2 to 3/4 of the area of the whole spike;
7 stages: the area of the dried spike accounts for more than 3/4 of the area of the whole spike.
The drug effect calculation method comprises the following steps:
results of the drug efficacy test and analysis:
table 6 results of field test for controlling wheat scab
Indoor toxicity measurement and field efficacy experiments show that the composition compounded by the cyclob rifluram, the carbendazim and the thiophanate-methyl has good control effect on wheat scab. The bactericidal composition or the preparation thereof obtained by compounding has remarkable prevention effect, and has the characteristics of high efficiency, broad spectrum, low residue, long lasting period, strong systemic property and the like; in addition, no drug damage to crops caused by the compound medicament is found in the test, which proves that the production cost and the use cost can be reduced and the sterilization composition or the preparation is safe to crops under the condition that the sterilization synergy of the obtained sterilization composition or preparation is improved.
While the invention has been described in detail in terms of the general description and the specific embodiments, it will be apparent to those skilled in the art that various modifications and improvements can be made thereto without departing from the spirit of the invention.

Claims (10)

1. The pesticide composition is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is cyclopurifiham, and the active ingredient B is selected from any one of carbendazim and thiophanate-methyl.
2. The pesticide composition as set forth in claim 1, wherein the mass ratio of the active ingredient a to the active ingredient B is 1:42 to 50:1.
3. The pesticide composition as set forth in claim 1, wherein the active ingredient B is carbendazim, and the mass ratio of the active ingredient cyclob rifluram to carbendazim is 1:42 to 42:1;
preferably, the mass ratio of the active ingredients of the cyclob rifluram to the carbendazim is 1:20-25:1.
4. The pesticide composition as set forth in claim 1, wherein the active ingredient B is thiophanate-methyl, and the mass ratio of the cyclopurifly to the thiophanate-methyl is 1:32-32:1;
preferably, the mass ratio of the active ingredients of the cyclob (r) fluoride to the thiophanate-methyl is 1:25-32:1.
5. A pesticide composition as set forth in claim 1 wherein the weight ratio of said active ingredient a to said active ingredient B is from 0.5% to 80% of the total weight of said pesticide composition, based on 100% by weight of the total weight of said pesticide composition.
6. A pesticide composition as set forth in claim 1, wherein said pesticide composition comprises, in addition to the active ingredient, other auxiliary ingredients selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezes, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.
7. The pesticide composition of claim 1, wherein the pesticide composition can be prepared into a formulation comprising a suspending agent, an aqueous emulsion, a microemulsion, an emulsifiable concentrate, a dispersible oil suspending agent, a soluble agent, a water dispersible granule, a wettable powder, a granule, a seed treatment suspending agent or a seed treatment microcapsule suspending agent.
8. The pesticide composition of claim 7, wherein the formulation is a suspension, wettable powder or water dispersible granule.
9. Use of the pesticidal composition according to any one of claims 1 to 8 for controlling plant diseases.
10. The use according to claim 9, wherein the disease is wheat scab, melon wilt.
CN202311444449.2A 2023-11-02 2023-11-02 Pesticide composition and application thereof Pending CN117481126A (en)

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