CN117598312A - Sterilization composition containing cyclopurifiuram and application thereof - Google Patents

Sterilization composition containing cyclopurifiuram and application thereof Download PDF

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Publication number
CN117598312A
CN117598312A CN202311557748.7A CN202311557748A CN117598312A CN 117598312 A CN117598312 A CN 117598312A CN 202311557748 A CN202311557748 A CN 202311557748A CN 117598312 A CN117598312 A CN 117598312A
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active ingredient
prochloraz
bactericidal composition
mass ratio
agents
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葛尧伦
葛晓甜
王良清
杨志鹏
吕文东
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Qingdao Hailier Biotechnology Co ltd
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Qingdao Hailier Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Agronomy & Crop Science (AREA)
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  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a bactericidal composition containing Cycloutrifluram and application thereof, wherein the active ingredients of the bactericidal composition comprise Cycloutrifluram and prochloraz or salts thereof, and the mass ratio of the Cycloutrifluram to the prochloraz or the salts thereof is 1:50-54:1. The bactericidal composition has remarkable control effect on wheat scab, can effectively slow down disease development, reduces the use amount of pesticides, and is safe to the environment.

Description

Sterilization composition containing cyclopurifiuram and application thereof
Technical Field
The invention belongs to the technical field of pesticide sterilization, and discloses a bactericidal composition containing cyclopurifiham and application thereof.
Background
Wheat scab caused by fusarium graminearum (Fusarium graminearum Schw) is one of the main diseases in the middle and downstream winter wheat regions and the south China winter wheat region and the northeast spring wheat region of China.
In recent years, due to the combined operation of the harvester, the returning of the straws to the field in the two-crop area of corn and wheat and the change of climate and irrigation conditions, the occurrence area of wheat scab is continuously enlarged, and particularly the occurrence area of wheat scab in North China is increasingly serious, so that the wheat scab becomes a problem to be solved in agricultural production.
Because some medicaments can produce synergistic effect after being compounded, the compounding benefit of the bactericide is increasingly important. The scientific compound bactericide not only can reduce the risk of drug resistance of pathogenic bacteria, but also can achieve better control effect than one-taste increase of single dose of bactericide, and can greatly reduce the difficulty of developing a novel bactericide. However, whether the synergistic effect is generated after the compounding is difficult to expect or not is found by the applicant through an indoor test, the cyclopurifluham and prochloraz or the salt thereof have obvious synergistic effect on wheat scab under a proper mass ratio, and the bactericidal composition with good control effect on the wheat scab is obtained through further verification through a field efficacy test, and the bactericidal composition compounded by the cyclopurifluham and prochloraz or the salt thereof and the application of the bactericidal composition for controlling the wheat scab are not reported.
Disclosure of Invention
Based on the problems, the invention provides a bactericidal composition which has excellent control effect on plant diseases, has obvious synergism on wheat scab, can effectively slow down disease spread, slow down the development of pathogen resistance, reduce the dosage of pesticides, is safe to crops and is environment-friendly.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the bactericidal composition containing the cycloprifluram comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is cycloprifluram, the active ingredient B is prochloraz or a salt thereof, and the mass ratio of the active ingredient A to the active ingredient B is 1:50-54:1;
further, the prochloraz salt is selected from prochloraz manganese salt or prochloraz copper salt;
further, the active ingredient B is prochloraz, and the mass ratio of the active ingredient A to the active ingredient B is 1:32-48:1;
the active ingredient B is prochloraz manganese chloride, and the mass ratio of the active ingredient A to the active ingredient B is 1:50-54:1;
the active ingredient B is prochloraz copper salt, and the mass ratio of the active ingredient A to the active ingredient B is 1:32-40:1.
Furthermore, the active ingredient B is prochloraz, and the mass ratio of the active ingredient A to the active ingredient B is 1:16-32:1;
the active ingredient B is prochloraz manganese chloride, and the mass ratio of the active ingredient A to the active ingredient B is 1:32-32:1;
the active ingredient B is prochloraz copper salt, and the mass ratio of the active ingredient A to the active ingredient B is 1:16-32:1.
Further, based on 100wt% of the total weight of the bactericidal composition, the total weight of the active ingredients of cyclob rifluram and prochloraz or salts thereof accounts for 1-70% of the total weight of the bactericidal composition.
Further, the bactericidal composition comprises an auxiliary component which is acceptable in pesticide besides an active component, wherein the auxiliary component is selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers;
further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium succinate, alpha-olefin sulfonate, alkylphenol ethoxylate, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrating agent F; and/or
The dispersing agent is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol ethoxylate methyl ether condensate sulfate, fatty amine ethoxylate, glycerin fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and/or
The emulsifier is one or more selected from calcium dodecyl benzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and/or
The thickener is one or more selected from xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and/or
Disintegrating agent the disintegrating agent is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and/or
The antifreezing agent is one or more selected from alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and/or
The defoamer is selected from C 10 -C 20 Saturated fatty acid compound, silicone oil, silicone compound, C 8 -C 10 One or more of the fatty alcohols; and/or
The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent oil, vegetable oil derivatives and water; and/or
The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, pinocembrane and 1, 2-benzisothiazolin-3-one; and/or
The stabilizer is one or more selected from disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talcum powder, montmorillonite and starch; and/or
The synergistic agent is selected from synergistic phosphorus and synergistic ether; and/or
The carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil and vegetable oil derivative.
Further, the bactericidal composition can be prepared into a preparation formulation acceptable in pesticides, wherein the preparation formulation is microemulsion, aqueous emulsion, suspending agent, dispersible oil suspending agent, solution, emulsifiable concentrate, suspending agent, microcapsule suspending agent, water dispersible granule, wettable powder and granule;
furthermore, the preparation formulation is water dispersible granule, wettable powder, suspending agent, aqueous emulsion, emulsifiable concentrate or microemulsion.
The invention also discloses the application of the bactericidal composition in preventing and controlling plant diseases.
Further, the plant disease is wheat scab.
The invention has the beneficial effects that: the bactericidal composition disclosed by the invention is capable of reasonably compounding different compounds with action mechanisms, relieving the problem of resistance of old medicaments, improving the control effect on wheat scab, reducing the dosage of pesticides, being safe to crops and being environment-friendly.
Detailed Description
In order to make the objects, advantages and technical solutions of the present invention more apparent, the technical solutions of the present invention will be explained by using the following preparation examples and specific examples, but the scope of the present invention should not be limited by the specific embodiments set forth herein.
Preparation example:
preparation example 1:27% Cyclobinifluram prochloraz water dispersible granule (8:1)
The formula comprises the following components: 24% of Cyclobutrifluram, 3% of prochloraz, 8% of sodium lignin sulfonate, 10% of sodium polycarboxylate, 2% of sodium dodecyl sulfate, 5% of white carbon black, 28% of starch and the balance of kaolin.
The preparation method comprises the following steps: uniformly mixing prochloraz and white carbon black according to the formula proportion, adding active ingredients into a carrier, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain a water dispersible granule product; or spraying water, granulating, drying, and sieving to obtain the final product.
Preparation example 2:30% Cyclobutrifluram prochloraz wettable powder (4:1)
The formula comprises the following components: 24% of cycloputrifluram, 6% of prochloraz, 2% of Guerbet alcohol polyoxyethylene ether, 15% of white carbon black, 6% of naphthalene sulfonate formaldehyde condensate, 10% of tea saponin, 2% of sodium dodecyl sulfate and the balance of kaolin;
the preparation method comprises the following steps: according to the formula proportion of the embodiment, the prochloraz and the white carbon black are uniformly mixed, the active ingredients are added into a carrier, and the surfactant and other functional additives are added into the carrier, mixed, crushed by air flow and then mixed to obtain the wettable powder product.
Preparation example 3:25% Cyclokutrifluram prochloraz emulsifiable concentrate (1:1)
The formula comprises the following components: 12.5% of Cyclobutrifluram, 12.5% of prochloraz, 20% of N-methylpyrrolidone, 10% of styrylphenol polyoxyethylene ether, 2% of calcium dodecyl benzene sulfonate, 10% of DMF and methyl oleate are used for the balance.
The preparation method comprises the following steps: according to the formula proportion, adding the active ingredients, the solvent and the cosolvent into a blending kettle, stirring to dissolve the active ingredients, adding the emulsifier, supplementing the rest with the residual solvent, stirring uniformly in the stirring kettle, and filtering to obtain the emulsifiable concentrate required by the invention.
Preparation example 4:7.5% Cyclobutrifluram prochloraz aqueous emulsion (4:1)
The formula comprises the following components: 6% of Cyclobutrifluram, 1.5% of prochloraz, 10% of xylene, 10% of cyclohexanone, 1% of tristyrylphenol ethoxylate phosphate ester, 8% of ethylene oxide-propylene oxide copolymer, 4% of ethylene glycol, 1% of glycerol, 0.05% of organosilicon defoamer, 0.1% of xanthan gum, 0.1% of sodium benzoate and water are used for balancing;
the preparation method comprises the following steps: according to the formula proportion of the embodiment, dissolving the effective components into a solvent, adding an emulsifying agent, dissolving into a uniform oil phase, and mixing deionized water and an antifreezing agent together to form a uniform water phase; adding the water phase into the oil phase under high-speed shearing to form a well-dispersed aqueous emulsion product.
Preparation example 5:9% Cyclobutrifluram prochloraz microemulsion (8:1)
The formula comprises the following components: 8% of Cyclobutrifluram, 1% of prochloraz, 20% of cyclohexanone, 12% of fatty alcohol polyoxyethylene ether, 3% of sorbitan oleate polyoxyethylene ether, 1% of fatty alcohol polyoxyethylene ether sulfate, 0.05% of organosilicon defoamer and deionized water, and the balance of the components are added;
the preparation method comprises the following steps: according to the formula proportion of the embodiment, the effective components, the solvent, the emulsifier and the like are uniformly mixed to prepare an oil phase, the antifreezing agent and the water are uniformly mixed to prepare a water phase, the oil phase is added into the water phase under the stirring state to be uniformly stirred, the shearing is continued for 10min, the defoaming agent is added into the water phase to be uniformly stirred to obtain small liquid drops with the oil phase particles of 0.01-0.1 micron, and the microemulsion product is prepared.
Preparation example 6:32% Cyclokutrifluram prochloraz manganese chloride suspension (15:1)
The formula comprises the following components: 30% of Cyclobutrifluram, 2% of prochloraz manganese, 1% of sodium sulfosuccinate, 2% of naphthalene sulfonate formaldehyde condensate, 3% of alkylphenol polyoxyethylene ether phosphate, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 5% of ethylene glycol, 0.01% of kathon, 0.5% of organosilicon defoamer and deionized water, and the balance is made up;
the preparation method comprises the following steps: according to the formula proportion, the active ingredients, the surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, the suspending agent product is obtained through homogenizing and filtering.
Preparation example 7:42% Cyclobutrifluram prochloraz manganese chloride wettable powder (2:1)
The formula comprises the following components: 28% of Cyclobutrifluram, 14% of prochloraz manganese, 10% of sodium lignin sulfonate, 8% of sodium polycarboxylate, 8% of starch, 5% of white carbon black and the balance of kaolin;
the preparation method comprises the following steps: the same as in preparation example 2.
Preparation example 8:35% Cyclobutrifluram prochloraz manganese chloride wettable powder (1:6)
The formula comprises the following components: 5% of Cyclobutrifluram, 30% of prochloraz manganese, 10% of sodium lignosulfonate, 2% of sodium dodecyl sulfate, 5% of sodium polycarboxylate, 7% of starch, 5% of white carbon black and kaolin, and the balance being filled;
the preparation method comprises the following steps: the same as in preparation example 2.
Preparation example 9:36% Cyclokutrifluham prochloraz copper salt suspension (8:1)
The formula comprises the following components: 32% of cycloputrifluram, 4% of prochloraz copper salt, 1% of alkylphenol ethoxylates, 4% of styrol polyoxyethylene ether phosphate, 1% of sodium lignin sulfonate, 0.25% of xanthan gum, 5% of ethylene glycol, 0.1% of kathon, 0.5% of silicone oil and deionized water, wherein the balance is filled;
the preparation method comprises the following steps: the same as in preparation example 6.
Preparation example 10:38% Cyclokutrifluham prochloraz copper salt suspension (1:1)
The formula comprises the following components: 19% of Cyclobutrifluram, 19% of prochloraz copper salt, 1% of fatty alcohol polyoxyethylene ether, 2% of glycerol fatty acid ester polyoxyethylene ether, 2% of styrol polyoxyethylene ether sulfate ester, 1% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.1% of carboxyethyl cellulose, 1% of sodium sorbate, 5% of glycerol, 0.5% of silicone oil and the balance of deionized water;
the preparation method comprises the following steps: the same as in preparation example 6.
Indoor activity experiment:
example 1: indoor activity test of wheat scab by compounding Cyclobutrifluram with prochloraz or prochloraz salt
The experimental basis is as follows: test reference NY/T1156.2-2006 agricultural industry standard "pesticide indoor bioassay test criteria section 2 of the people's republic of China: test plate method for inhibiting growth of pathogenic bacterial hyphae.
Test target: fusarium graminearum (Fusarium graminearum).
Instrument apparatus: a wet heat sterilization pot, an ultra-clean workbench, a constant temperature illumination incubator, an electric heating drum windproof drying box, a ten thousandth electronic balance, a pipetting gun, an alcohol lamp, a small beaker, a volumetric flask, a triangular flask, a culture dish (phi 9 cm), a puncher, an inoculator (phi 0.6 cm), a ruler and the like.
Test agent: prochloraz technical, cyclobutrifluram technical, prochloraz manganese salt technical and prochloraz copper salt technical.
And (3) preparation of a medicament mother solution: after the above raw materials are dissolved in a proper solvent, the raw materials are diluted by using 0.1% Tween 80 aqueous solution to prepare single-dose mother solutions respectively, and different proportions are designed according to the mixing purpose and the medicament activity, and each single dose and each group of proportion mixture are prepared into a required series of mass concentrations.
And (3) medicament treatment: under the aseptic operation condition, the PDA culture medium which is melted and sterilized in advance is quantitatively added into an aseptic conical flask according to the test treatment, 10mL of each prepared treatment liquid medicine is quantitatively sucked from low concentration to high concentration in sequence, the prepared treatment liquid medicines are respectively added into the conical flask, fully and uniformly shaken, and then the mixture is poured into a culture dish in equal quantity to prepare a medicine-containing flat plate with corresponding concentration. The test set up was run with a blank of 0.1% tween 80 in water without the addition of the agent, and 4 replicates per treatment.
Inoculating: cutting bacterial cake from colony edge with sterilized Fusarium graminearum under aseptic condition, inoculating bacterial cake in the center of medicated plate with inoculator, covering with bacterial cover, and culturing in constant temperature illumination incubator at 26deg.C.
Data investigation: the growth of pathogenic hyphae was investigated according to the growth of hyphae in a blank culture dish. Colony diameters were measured in centimeters (cm) using a ruler, diameters were measured once per colony using the cross-over method, averages were taken, and raw data was recorded for each treatment in duplicate.
Data statistics and analysis: according to the investigation result, the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested is calculated, wherein the unit is the calculated result of percentage (%) and two positions after decimal point are reserved.
D＝D 1 -D 2
Wherein:
d-colony growth diameter;
D 1 colony diameter;
D 2 -diameter of the bacterial cake.
Wherein:
i, hypha growth inhibition rate;
D 0 -the control colony increased in diameter;
D T -the agent-treated colonies increased in diameter.
Analysis by DPS statistical analysis system to obtain EC 50 And evaluating the activity of the test agent on the biological test material.
Grand cloud Pei method: evaluating the synergistic effect of the mixed medicaments according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient CTC is more than or equal to 120 and shows the synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
Co-toxicity coefficient (CTC value) of the blend was calculated:
wherein:
ati—actual measured virulence index of the mixture;
S-EC of Standard Agents 50 Milligrams per liter (mg/L);
M-EC of mixture 50 Units are milligrams per liter (mg/L).
TTI＝TI A ×P A +TI B ×P B
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A -a medicament virulence index;
P A -the percentage of agent a in the mix, in percent (%);
TI B -B agent virulence index;
P B -B agent in a mixPercent content, in percent (%).
Wherein:
ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The test results are shown in the following table:
TABLE 1 indoor Activity assay of Cyclokutrifluram and prochloraz on wheat scab
TABLE 2 indoor Activity test of Cyclokutrifluram and prochloraz manganese chloride complex on wheat scab
TABLE 3 indoor Activity test of Cyclokutrifluram and prochloraz copper salt on wheat scab
The indoor biological activity test results of the tables (tables 1-3) show that the cyclifluham and prochloraz or the salt thereof are reasonably compounded, and the compound shows excellent control effect on wheat scab at a certain mass ratio. When the mass ratio of the cyclopurifiuram to the prochloraz is 1:48, the co-toxicity coefficient (CTC) is less than 120, and the additive effect is shown on wheat scab; the mass ratio of the two is 1:32-48:1, and the co-toxicity coefficient (CTC) is more than 120 and shows synergistic effect; when the mass ratio of the cyclopurifiuram to the prochloraz manganese is 1:50-54:1, the co-toxicity coefficient (CTC) is greater than 120 and the synergistic effect is shown; the cyclopurifiuram and prochloraz copper salt are compounded to form an additive effect when the mass ratio is 1:48 and 48:1; the mass ratio of the two is 1:32-40:1, and the co-toxicity coefficient (CTC) is more than 120, thus the synergistic effect is shown.
Field efficacy test
Example 4: pesticide effect test for preventing and treating wheat scab in field
Test site: the eight-treasure white water flowing village wheat field in Pingzi city of Hubei province has flat test Tian Deshi, medium soil fertility and sandy loam soil texture. The previous crop is corn, and all the cultivation management conditions of the test communities are uniform and consistent, so that the method accords with the local scientific agricultural practice.
Test target: wheat scab.
Test crop: wheat (Ebei wheat 170).
And (3) test design: the test set up 8 treatments, 7 agent treatments, 1 clear water control, each treatment repeated 4 times, the test cells were in a random block arrangement.
Time of application: the test was performed with the first application of the wheat at the flowering period of day 1, 4, 2022 and with the 2 nd application at day 8, 4, 2022, for a total of 2 applications throughout the test.
Experimental investigation: the test is carried out on day 5 and 16 of 2022, 5 points are sampled in each district during investigation, 100 ears are investigated at each point, scab ears are investigated, the ears are classified according to the percentage of the area of the ears to the whole ear area, the number of ears of each stage and the total number of ears are recorded, and the control effect is calculated.
The grading method comprises the following steps:
level 0: the whole spike is free from diseases;
stage 1: the area of the dried spike accounts for less than 1/4 of the area of the whole spike;
3 stages: the area of the dried spike accounts for 1/4 to 1/2 of the area of the whole spike;
5 stages: the area of the dried spike accounts for 1/2 to 3/4 of the area of the whole spike;
7 stages: the area of the dried spike accounts for more than 3/4 of the area of the whole spike.
The drug effect calculation method comprises the following steps:
results of the drug efficacy test and analysis:
TABLE 4 results of field efficacy test for controlling wheat scab
Drug safety: after each application of the pesticide for 7d, field observation is carried out, and the symptoms such as dwarfing, fading, malformation and the like are not seen on the wheat stem leaves in the field observation test area, and the wheat grows well in the blank control area and the pesticide treatment area. The test agent has no phytotoxicity symptoms after application, and is safe for wheat growth.
As shown in Table 4, under the condition of a certain number of investigation ears, the number of wheat scab disease ears is not more than 30 ears after 3 compound medicaments are applied, the disease ear rate is between 4.30 and 5.95 percent, the disease index is between 1.46 and 2.36, and the prevention effect is 88.73 to 93.05 percent.
The test result shows that the sterilization composition can effectively prevent and treat wheat scab in the critical period of wheat heading and flower-lifting, and the prevention and treatment effects are above 88% and are obviously higher than that of a blank control after 2 times of administration. After the pesticide is applied, the field wheat does not have any phytotoxicity symptoms such as chlorosis, malformation and the like, has good field growth vigor and is safe for the growth of the wheat.
While the invention has been described in detail in terms of the general description and the specific embodiments, it will be apparent to those skilled in the art that various modifications and improvements can be made thereto without departing from the spirit of the invention.

Claims (10)

1. The bactericidal composition containing the cycloprifluram is characterized in that active ingredients of the bactericidal composition comprise an active ingredient A and an active ingredient B, wherein the active ingredient A is cycloprifluram, the active ingredient B is prochloraz or a salt thereof, and the mass ratio of the active ingredient A to the active ingredient B is 1:50-54:1.
2. The bactericidal composition of claim 1 wherein the prochloraz salt is selected from prochloraz manganese or prochloraz copper salts.
3. The bactericidal composition of claim 1, wherein the active ingredient B is prochloraz, and the mass ratio of the active ingredient a to the active ingredient B is 1:32-48:1;
the active ingredient B is prochloraz manganese chloride, and the mass ratio of the active ingredient A to the active ingredient B is 1:50-54:1;
the active ingredient B is prochloraz copper salt, and the mass ratio of the active ingredient A to the active ingredient B is 1:32-40:1.
4. The bactericidal composition according to claim 1, wherein the active ingredient B is prochloraz, and the mass ratio of the active ingredient a to the active ingredient B is 1:16-32:1;
the active ingredient B is prochloraz manganese chloride, and the mass ratio of the active ingredient A to the active ingredient B is 1:32-32:1;
the active ingredient B is prochloraz copper salt, and the mass ratio of the active ingredient A to the active ingredient B is 1:16-32:1.
5. The bactericidal composition of claim 1, wherein the total weight of the active ingredients, cycloprifluram and prochloraz or salts thereof, is 1 to 70wt% of the total weight of the bactericidal composition, based on the total weight of the bactericidal composition taken as 100 wt%.
6. A fungicidal composition according to claim 1, wherein the fungicidal composition comprises, in addition to the active ingredient, an agrochemically acceptable auxiliary ingredient selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, freezing point depressants, antifoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers.
7. The bactericidal composition of claim 1, wherein the bactericidal composition is formulated into a pesticidally acceptable dosage form, the dosage form being a microemulsion, an aqueous emulsion, a suspension, a dispersible oil suspension, a solution, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water dispersible granule, a wettable powder, or a granule.
8. The bactericidal composition of claim 7, wherein the formulation is in the form of water dispersible granules, wettable powder, suspension, emulsion in water, emulsifiable concentrate or microemulsion.
9. Use of the fungicidal composition according to any one of claims 1 to 8 for controlling plant diseases.
10. The use according to claim 9, wherein the plant disease is wheat scab.
CN202311557748.7A 2023-11-22 2023-11-22 Sterilization composition containing cyclopurifiuram and application thereof Pending CN117598312A (en)

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CN202311557748.7A CN117598312A (en) 2023-11-22 2023-11-22 Sterilization composition containing cyclopurifiuram and application thereof

Applications Claiming Priority (1)

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CN202311557748.7A CN117598312A (en) 2023-11-22 2023-11-22 Sterilization composition containing cyclopurifiuram and application thereof

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CN117598312A true CN117598312A (en) 2024-02-27

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