CN104957145A - Sterilization composition and use thereof - Google Patents
Sterilization composition and use thereof Download PDFInfo
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- CN104957145A CN104957145A CN201510292410.2A CN201510292410A CN104957145A CN 104957145 A CN104957145 A CN 104957145A CN 201510292410 A CN201510292410 A CN 201510292410A CN 104957145 A CN104957145 A CN 104957145A
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- shen piperazine
- piperazine mycin
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Abstract
The invention discloses a sterilization composition and a use thereof and relates to the technical field of pesticide compounding. The sterilization composition contains active ingredients of phenazine-1-carboxylic acid A and triazole bactericide B. The triazole bactericide B is epoxiconazole, flusilazole or tetraconazole. A weight ratio of phenazine-1-carboxylic acid A to triazole bactericide B is in a range of 1: 60 to 60: 1. The compounded active ingredients A and B have obvious synergism and can effectively reduce a use amount of a single reagent. The sterilization composition has good effects of enlarging a sterilization spectrum and delaying plant resistance to bactericide, has a long persistent period and has an obvious popularization value. Auxiliary components allowed by other pesticides can be added into the sterilization composition so that any dosage form is prepared. The sterilization composition can effectively prevent and treat rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, banana black spot, banana black-blight or botrytis cinerea.
Description
Technical field
The present invention relates to a kind of bactericidal composition and application thereof, refer to a kind of be active ingredient bactericidal composition and the application thereof of Shen piperazine mycin and triazole bactericidal agent especially, belong to technical field of pesticide combination.
Background technology
In agricultural production, can crops healthy growth, except affecting by worm, crop smothering, also very important to the control of disease.Along with reinforcement and the sustainable development implementation of environmental protection ideas, the inexorable trend efficient, low toxicity, high activity, low-residual have become Agrochemicals.But, even if efficient germicide, be used alone for a long time, also easily make disease develop immunity to drugs, cause the problems such as dosage increasing, preventive effect reduction, lasting period shortening, be unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, reduces the consumption of chemical pesticide, improves drug effect, becomes the problem that plant protection art is badly in need of solving.
Banded sclerotial blight is also known as moire disease, and popular name spends sufficient stalk, rotten pin pest, features spot.Be infected by Rhizoctonia solani Kuhn to fall ill, how occur under high temperature, super-humid conditions.Banded sclerotial blight causes harm seriously in South Rice Region of China, is one of Major Diseases in current Rice Production.This disease makes paddy rice not ear, or the not plump paddy of heading is more, and grain heavily declines.
False smut, calls blue or green powder disease, pseudo-smut, belongs to fungal disease.Along with some popularization of compact water rice varieties of short stem and the raising of Fertilization Level, this sick generation is more and more outstanding.The empty flat grain of sick fringe significantly increases, generally can the underproduction 5% ~ 10% after morbidity.False smut mainly occurs at Rice Heading blooming stage, and harm fringe upper part grain, every fringe 1 ~ 2 at least, serious reaches 20 ~ 30.
Epoxiconazole (epoxiconazole), also known as epoxy bacterium azoles, chemical name: (2RS, 3RS)-1-[3-(2 monochloro-benzene base)-2,3 one epoxy-2-(4 phenyl-monofluoride base) propyl group] one 1 hydrogen one 1,2,4 one triazoles.Epoxiconazole is fluorine-containing triazole bactericidal agent, has the feature such as wide spectrum, lasting period length.Belong to C-14 demethylation enzyme inhibitor in sterol biosynthesis, not only there is good protection, treat and root out activity, and there is interior suction and preferably residual activity.
Flusilazole (flusilazole) is also known as Nustar, Olymp, Punch, lucky star, gram bacterium star, Qiu Fu, chemical name: two (4 phenyl-monofluoride base) methyl (support of 1H-1,2,4--azoles-l-base methylene) silane.Flusilazole is first fluorine-containing siliceous, highly active triazole bactericidal agent again in the world, belongs to wide spectrum systemic fungicide.There is systemic activity, protection and therapeutic action.Destroy and stop the biosynthesis of ergosterol, cause cell membrane not formed, make germ dead.
Tetraconazole (tetraconazole), also known as: a Mike, fluorine ether azoles, chemical name:; (±)-2-(2,4-dichlorophenyl)-3-(1H-1,2,4-triazol-1-yl) propyl group-1,1,2,2 ,-tetrafluoro ethyl ether.Tetraconazole belongs to second generation triazole bactericidal agent, fluorine-containing in molecular structure, bactericidal activity than be the 2-3 of the first generation doubly, fungicidal spectrum is wide, efficient, the lasting period reaches 4-6 week, has protection and therapeutic action, and has good Uptake and translocation performance.
Shen piperazine mycin, its structural formula is as shown in (I), Shen piperazine mycin is a kind of antibiotic secreted through biological culture by pseudomonas fluorescens M18, has wide spectrum Suppressing phytopathogens and the bactericide of the dual-use function of Promoting plant growth effect simultaneously, has wide spectrum, efficiently feature.
Structural formula (I) Shen piperazine mycin
Frequent use containing single-activity component bactericide, cause most germs to create pesticide resistance, preventive effect is unsatisfactory, must continue to increase the dosage of agricultural chemicals, just can reach the object of degerm.Although Shen piperazine mycin, epoxiconazole, Flusilazole, tetraconazole are efficient as bactericide, but its single dose Long-Time Service, easily make disease develop immunity to drugs, cause the problem that dosage strengthens, preventive effect reduces, the lasting period shortens, be unfavorable for environment sustainable development.Therefore, be badly in need of the sterilised products that a kind of novel product substitutes single-activity component, increase the resistance risk that drug effect delays disease, reduce economic loss.
Summary of the invention
It is reasonable that the technical problem to be solved in the present invention is to provide a kind of component, and synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, not easily develops immunity to drugs, to the bactericidal composition of crop safety and application thereof.
For solving the problems of the technologies described above, the invention provides technical scheme as follows:
Bactericidal composition of the present invention, its active ingredient is Shen piperazine mycin A and triazole bactericidal agent B, and the weight ratio of Shen piperazine mycin and triazole bactericidal agent is 1:60 ~ 60:1, and being preferably weight ratio is 1:30 ~ 30:1, and more preferably weight ratio is 1:15 ~ 15:1.
In bactericidal composition of the present invention, described triazole bactericidal agent B is fluorine-containing triazole bactericidal agent, is preferably any one in epoxiconazole, Flusilazole or tetraconazole etc.
Pesticidal preparations auxiliary element can also be contained, to make the formulation being applicable to agriculturally using in bactericidal composition of the present invention.Described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is dry suspending agent (DF), wetting powder (WP), water dispersible granules (WDG), suspending agent (SC) etc.
A wetting powder (WP) is can homodisperse solid powder preparation in water.Wetting powder of the present invention except adding as white carbon in former medicine, precipitated calcium carbonate, potter's clay, diatomite, outside the thinners such as attapulgite or inert substance, also have ionic or nonionic surface active agent (wetting agent, dispersant), wetting agent such as, alkylbenzenesulfonate (DBS-Na), alkylnaphthalene sulfonate (pulling open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1 ~ 30%, dispersant is as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, polyoxyethylene poly-oxygen propylene aether block copolymer, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate (SOPA), PVA, CMC content 0.1 ~ 30%, also containing one or more materials among penetrating agent JFC, azone, thiophene ketone, penetrant t, content 0.3 ~ 30%, other auxiliary agents such as stabilizing agent, foam inhibitor, anticaking agent can also be contained.
B water dispersible granules (WDG) very fast disintegration in water, dispersion can form the granule of higher suspension dispersion.By active component, the key element such as auxiliary agent and the carrier compositions such as wetting agent, dispersant, interleaving agent, disintegrant, stabilizing agent, adhesive.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before granulation) is analogous to wetting powder and suspending agent substantially.
C suspending agent (SC) is not water soluble solid agricultural chemicals or the dispersion of immiscible liquids agricultural chemicals in water or oil.Obtained through soybean dietary fiber by active component, auxiliary agent (wetting dispersing agent, thickener, stabilizing agent, pH adjusting agent, defoamer, antifreezing agent).Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, one or more in alkylphenol-polyethenoxy base phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensed phosphate etc., content 0.1 ~ 12%; Thickener and stabilizing agent can be one or more in xanthan gum (XG), polyvinyl alcohol (PVA), Magnesiumaluminumsilicate etc., content 0.05 ~ 0.5%; PH adjusting agent and defoamer can optionally add in right amount.
D dry suspending agent (Dry Flowable, be called for short DF), be can very fast disintegration in water, dispersion formation higher suspension dispersion granule.Preparation method: the preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before dehydrating) is consistent with suspending agent.
Bactericidal composition of the present invention is applicable to the pesticidal preparations preparing controlling plant diseases.Be particularly useful for making the pesticidal preparations of the same domain diseases such as the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew banana freckle, banana black disease disease, graw mold of tomato.
Bactericidal composition of the present invention can finished product preparation form provide, namely in bactericidal composition, each material mixes, or the composition of bactericidal composition also can provide with unit dose form, directly directly mix in bucket or tank before using, be then diluted to required concentration.
Bactericidal composition of the present invention is applied to the method for controlling disease, active principle A Shen piperazine mycin and B component triazole bactericidal agent (epoxiconazole, Flusilazole, tetraconazole) are used with the weight ratio of 1:60 ~ 60:1, preferably using weight ratio is 1:30 ~ 30:1, more preferably using weight ratio is 1:15 ~ 15:1, and it is used after can mixing in proportion, use in proportion simultaneously or use successively in proportion.Concrete method of application can be powder injection process, spray-on process, smog method, fumigating system and seed treatment and soil treatment, ultra low volume spraying, mixes fertilizer, spreads fertilizer over the fields, fills with root.
Compared with prior art, beneficial effect of the present invention is:
1, compared with single dose, composite have obvious synergistic function, improves control efficiency;
2, composition is made up of the active ingredient that two kinds of mechanism of action are different, and bactericidal effect is good, is conducive to overcoming and delay the drug-fast generation of germ;
3, medicament mixture decreases dosage, thus reduces cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment adds protective effect, and drug cost is low, and its activity and bactericidal effect are not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.
Embodiment
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, will be described in detail with specific embodiment below, but the present invention is limited to absolutely not these examples.The following stated is only the good embodiment of the present invention, only in order to explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
The combination of active principles of different agricultural chemicals is made agricultural chemicals, is current development novel pesticide and the one of preventing and treating agriculturally resistance germ effectively and efficiently mode.After the pestsides synthesis of different cultivars, usually show a large amount of three kinds of type of action: summation action, synergistic effect and antagonism.Compound synergic is well filled a prescription, and can significantly improve actual control efficiency, and reducing the usage amount of agricultural chemicals, thus contribute to delaying the drug-fast generation speed of germ, is the important means of integrated control disease.
The present composition with Shen piperazine mycin (A) for a kind of active ingredient, triazole bactericidal agent (B) is another active ingredient, triazole bactericidal agent (B) is fluorine-containing triazole bactericidal agent, is preferably epoxiconazole, Flusilazole or tetraconazole.Combine between them and have obvious synergistic function to plant disease, and be not only the simple superposition of two kinds of pharmacy effects, this can clearly recognize in virulence test result indoor.
In order to prevent and treat the sigatoka in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and epoxiconazole, and concrete grammar is:
Test adopts sigatoka to be tested object.
Former medicine is mixed with the test medicine of needs, with reference to mycelial growth rate method, the preparation of bacterial classification: pour about 10mLPDA medium in sterilized medium into.Aseptically, get a mycelia block from the strain inclined plane of sigatoka bacterium, be placed in the middle of culture dish.Continuous culture several generations, then before the test 3d, inoculation once, cultivate under 24 ~ 26 DEG C of conditions, for subsequent use.The making of toxic flat board, after first being dissolved by the PDA medium prepared, places cooling.Aseptically, added by liquid 1mL in sterilized culture dish, then add with pipette, extract 9mL medium in the culture dish of existing liquid, rocking gently shakes up (left 3 circles, right 3 circles), and it is dull and stereotyped uniformly to make thin and thick.Connect bacterium: off-the-shelf pathogen card punch is made bacterium cake, then move on the pastille flat board as solidified with transfer needle picking bacterium cake, mycelia one faces down.Mark, puts in 24 ~ 26 DEG C of incubators and cultivates.Treat that control group colony diameter grows to about 6cm, right-angled intersection method investigates other data processed and record.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 1 Shen piperazine mycin and the composite Toxicity Determination to sigatoka of epoxiconazole
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shen piperazine mycin (A) | —— | Y＝3.6303+0.8267X | 45.3644 | —— |
| Epoxiconazole (B1) | —— | Y＝3.9332+0.5542X | 84.1322 | —— |
| A:B1 | 1:60 | Y＝4.4699+0.3359X | 37.8592 | 120.46 |
| A:B1 | 1:50 | Y＝4.0943+0.5000X | 64.7725 | 123.30 |
| A:B1 | 1:30 | Y＝3.9946+0.5537X | 65.4543 | 125.08 |
| A:B1 | 1:25 | Y＝4.4356+0.3623X | 36.0161 | 126.62 |
| A:B1 | 1:15 | Y＝3.8988+0.7061X | 36.2556 | 128.83 |
| A:B1 | 1:9 | Y＝4.4576+0.3513X | 34.9919 | 130.33 |
| A:B1 | 1:1 | Y＝3.9079+0.7062X | 35.1847 | 130.87 |
| A:B1 | 8:1 | Y＝4.4600+0.3541X | 33.4997 | 136.61 |
| A:B1 | 15:1 | Y＝4.4650+0.3487X | 34.2113 | 133.30 |
| A:B1 | 25:1 | Y＝4.4673+0.3428X | 35.7983 | 127.39 |
| A:B1 | 30:1 | Y＝3.8993+0.7049X | 36.4282 | 126.41 |
| A:B1 | 49:1 | Y＝3.9141+0.6475X | 47.5422 | 123.98 |
| A:B1 | 60:1 | Y＝4.0969+0.4933X | 67.7449 | 122.47 |
Toxicity Determination result table 1 shows, at weight ratio 1:60-60:1, in the scope of particularly 1:30-30:1, especially Shen piperazine mycin and epoxiconazole is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:15-15:1, composite synergistic effect is more obvious, and control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the leaf spot of peanut in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and Flusilazole, and concrete grammar is:
Test adopts leaf spot of peanut to be tested object.
Former medicine is mixed with the test medicine of needs, with reference to mycelial growth rate method, the preparation of bacterial classification: pour about 10mLPDA medium in sterilized medium into.Aseptically, get a mycelia block from the strain inclined plane of leaf spot of peanut germ, be placed in the middle of culture dish.Continuous culture several generations, then before the test 3d, inoculation once, cultivate under 24 ~ 26 DEG C of conditions, for subsequent use.The making of toxic flat board, after first being dissolved by the PDA medium prepared, places cooling.Aseptically, added by liquid 1mL in sterilized culture dish, then add with pipette, extract 9mL medium in the culture dish of existing liquid, rocking gently shakes up (left 3 circles, right 3 circles), and it is dull and stereotyped uniformly to make thin and thick.Connect bacterium: off-the-shelf pathogen card punch is made bacterium cake, then move on the pastille flat board as solidified with transfer needle picking bacterium cake, mycelia one faces down.Mark, puts in 24 ~ 26 DEG C of incubators and cultivates.Treat that control group colony diameter grows to about 6cm, right-angled intersection method investigates other data processed and record.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 2 Shen piperazine mycin and the composite Toxicity Determination to leaf spot of peanut of Flusilazole
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shen piperazine mycin (A) | —— | Y＝3.6303+0.8267X | 45.3644 | —— |
| Flusilazole (B2) | —— | Y＝3.9299+0.5269X | 107.3661 | —— |
| A:B2 | 1:60 | Y＝4.0500+0.4897X | 87.0583 | 120.62 |
| A:B2 | 1:50 | Y＝4.0483+0.4941X | 84.3429 | 123.97 |
| A:B2 | 1:30 | Y＝4.0474+0.4980X | 81.8182 | 125.66 |
| A:B2 | 1:25 | Y＝4.0465+0.5019X | 79.4026 | 128.43 |
| A:B2 | 1:15 | Y＝4.0456+0.5066X | 76.5773 | 129.12 |
| A:B2 | 1:9 | Y＝4.0455+0.5138X | 72.1023 | 130.91 |
| A:B2 | 1:1 | Y＝4.0274+0.5767X | 48.5665 | 130.97 |
| A:B2 | 8:1 | Y＝4.1063+0.5705X | 36.8511 | 131.06 |
| A:B2 | 15:1 | Y＝4.1251+0.5647X | 35.4158 | 132.39 |
| A:B2 | 25:1 | Y＝4.1245+0.5694X | 34.4837 | 134.03 |
| A:B2 | 30:1 | Y＝4.1430+0.5497X | 36.2265 | 127.12 |
| A:B2 | 49:1 | Y＝4.1430+0.5497X | 36.6753 | 124.66 |
| A:B2 | 60:1 | Y＝4.1423+0.5438X | 37.7727 | 120.78 |
Toxicity Determination result table 2 shows, at weight ratio 1:60-60:1, in the scope of particularly 1:30-30:1, especially Shen piperazine mycin and Flusilazole is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:15-15:1, composite synergistic effect is more obvious, and control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the powdery mildew of cucumber in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and tetraconazole, and concrete grammar is:
Test adopts powdery mildew of cucumber to be tested object.
Former medicine is mixed with the test medicine of needs, test method (pot-culture method) is with reference to " People's Republic of China's agricultural industry criteria ".Wholeheartedly cucumber seedling is for subsequent use to select consistent three leaves of growing way, single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different agents combination, concentration arranges also different, and bacteriostasis rate sets in the scope of 5% ~ 95%).With spray-on process by medicine liquid spray on cucumber seedling for subsequent use, naturally dry rear for subsequent use, test set not containing the process of medicament as blank.The fresh sporozoite of Powdery Mildew produced in 24h on morbidity cucumber leaves evenly is tiltedly fallen to being inoculated on the 2-3 leaf phase potted plant cucumber seedling of process, often processes 3 basins.Protectiveness test 24h after chemicals treatment, curative tests is 24h inoculation before chemicals treatment, then puts 28 degrees Celsius of constant temperature lighting box and cultivates.According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 3 Shen piperazine mycin and the composite Toxicity Determination to powdery mildew of cucumber of tetraconazole
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shen piperazine mycin (A) | —— | Y＝3.6303+0.8267X | 45.3644 | |
| Tetraconazole (B3) | —— | Y＝3.5271+0.7508X | 91.5936 | |
| A:B3 | 1:60 | Y＝3.8260+0.6283X | 73.8804 | 121.9 |
| A:B3 | 1:50 | Y＝3.8251+0.6299X | 73.3287 | 122.44 |
| A:B3 | 1:30 | Y＝3.8246+0.6326X | 72.0938 | 123.00 |
| A:B3 | 1:25 | Y＝3.8278+0.6335X | 70.8386 | 124.38 |
| A:B3 | 1:15 | Y＝3.8309+0.6369X | 68.5011 | 125.65 |
| A:B3 | 1:9 | Y＝3.8314+0.6325X | 70.3863 | 128.81 |
| A:B3 | 1:1 | Y＝3.8709+0.6762X | 46.7667 | 129.41 |
| A:B3 | 8:1 | Y＝3.8803+0.7165X | 36.7088 | 130.44 |
| A:B3 | 15:1 | Y＝3.8795+0.7239X | 35.3055 | 132.18 |
| A:B3 | 25:1 | Y＝3.8779+0.7313X | 34.2230 | 134.64 |
| A:B3 | 30:1 | Y＝3.8807+0.7193X | 35.9815 | 127.68 |
| A:B3 | 49:1 | Y＝3.8763+0.7147X | 37.3397 | 122.26 |
| A:B3 | 60:1 | Y＝3.8769+0.7126X | 37.6843 | 120.92 |
Toxicity Determination result table 3 shows, at weight ratio 1:60-60:1, in the scope of particularly 1:30-30:1, especially Shen piperazine mycin and tetraconazole is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:15-15:1, composite synergistic effect is more obvious, and control efficiency is excellent and be better than single dose kind.
Toxicity Determination result table 1-table 3 shows, at weight ratio 1:60-60:1, in the scope of particularly 1:30-30:1, Shen piperazine mycin and epoxiconazole, Shen piperazine mycin and Flusilazole, Shen piperazine mycin and tetraconazole is composite has obvious synergistic effect to plant disease, control efficiency is excellent and be better than single dose kind.
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, within the scope of weight ratio 1:60 ~ 60:1, build the embodiment that Shen piperazine mycin is composite with epoxiconazole, Flusilazole or tetraconazole respectively, be described in detail, but the present invention is limited to absolutely not these examples.
In addition, inventor also found through experiments, Shen piperazine mycin respectively with the composite effect of epoxiconazole, Flusilazole or tetraconazole, be better than the composite effect of other triazole bactericidal agents and Shen piperazine mycin.In order to this problem is clearly described, within the scope of weight ratio 1:60 ~ 60:1, build other triazole bactericidal agents and the composite comparative example of Shen piperazine mycin, be described in detail.But because length is limited, representative several comparative examples can only be enumerated.It should be pointed out that and be not only confined in limited comparative example.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is wetting powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules.In all formulas, percentage is all weight percentage.The processing technology of the various formulation of the present composition is prior art, can change to some extent according to different situations.
Below in conjunction with embodiment, content of the present invention is described further.
One: Shen piperazine mycin and epoxiconazole composite
Embodiment 1:30.5% Shen piperazine mycin epoxiconazole wetting powder (1:60)
Component: Shen piperazine mycin 0.5%, epoxiconazole 30%, neopelex 6%, sodium lignin sulfonate 12%, white carbon 20%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 30.5% Shen piperazine mycin epoxiconazole wetting powder.
Comparative example 1:30.5% polyoxin epoxiconazole wetting powder (1:60)
Component: polyoxin 0.5%, epoxiconazole 30%, neopelex 6%, sodium lignin sulfonate 12%, white carbon 20%, talcum powder complements to 100%.
Preparation method: the same.
Comparative example 2:30.5% Shen piperazine mycin propiconazole wetting powder (1:60)
Component: Shen piperazine mycin 0.5%, propiconazole 30%, neopelex 6%, sodium lignin sulfonate 12%, white carbon 20%, talcum powder complements to 100%.
Preparation method: the same.
Embodiment 2:46% Shen piperazine mycin epoxiconazole water dispersant (1:45)
Component: Shen piperazine mycin 1%, epoxiconazole 45%, polycarboxylic acids sodium 5%, neopelex 4%, white carbon 8%, kaolin supplies 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 46% Shen piperazine mycin epoxiconazole water dispersant.
Comparative example 3:46% jinggangmeisu epoxiconazole water dispersant (1:45)
Component: jinggangmeisu 1%, epoxiconazole 45%, polycarboxylic acids sodium 5%, neopelex 4%, white carbon 8%, kaolin supplies 100%.
Preparation method: the same.
Embodiment 3:31% Shen piperazine mycin epoxiconazole wetting powder (1:30)
Component: Shen piperazine mycin 1%, epoxiconazole 30%, neopelex 3%, polycarboxylic acids sodium 4%, white carbon 8%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 31% Shen piperazine mycin epoxiconazole wetting powder.
Embodiment 4:25% Shen piperazine mycin epoxiconazole suspension concentrate (1:24)
Component: Shen piperazine mycin 1%, epoxiconazole 24%, fatty alcohol-polyoxyethylene ether 3%, polycarboxylic acids sodium 4%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin epoxiconazole suspension concentrate can be obtained.
Comparative example 4:25% Shen piperazine mycin hexaconazole suspending agent (1:24)
Component: Shen piperazine mycin 1%, own azoles alcohol 24%, fatty alcohol-polyoxyethylene ether 3%, polycarboxylic acids sodium 4%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: the same.
Embodiment 5:38% Shen piperazine mycin epoxiconazole water dispersant (1:18)
Component: Shen piperazine mycin 2%, epoxiconazole 36%, polycarboxylic acids sodium 6%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 10%, kaolin supplies 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 38% Shen piperazine mycin epoxiconazole water dispersant.
Embodiment 6:16% Shen piperazine mycin epoxiconazole dry suspending agent (1:15)
Component: Shen piperazine mycin 1%, epoxiconazole 15%, sodium lignin sulfonate 6%, lignin sodium naphthalene sulfonate 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 6%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine mycin epoxiconazole dry suspending agent.
Embodiment 7:20% Shen piperazine mycin epoxiconazole water dispersant (1:1)
Component: Shen piperazine mycin 10%, epoxiconazole 10%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 20% Shen piperazine mycin epoxiconazole water dispersant.
Comparative example 5:20% jinggangmeisu epoxiconazole water dispersant (1:1)
Component: jinggangmeisu 10%, epoxiconazole 10%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: the same.
Comparative example 6:20% polyoxin epoxiconazole water dispersant (1:1)
Component: polyoxin 10%, epoxiconazole 10%, neopelex 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 10%, talcum powder complements to 100%.
Preparation method: the same.
Embodiment 8:31% Shen piperazine mycin epoxiconazole wetting powder (30:1)
Component: Shen piperazine mycin 30%, epoxiconazole 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 5%, white carbon 10%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 31% Shen piperazine mycin epoxiconazole wetting powder.
Comparative example 7:31% Shen piperazine mycin propiconazole wetting powder (30:1)
Component: Shen piperazine mycin 30%, propiconazole 1%, lauryl sodium sulfate 3%, lignin sodium naphthalene sulfonate 5%, white carbon 10%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 9:30.5% Shen piperazine mycin epoxiconazole suspension concentrate (60:1)
Component: Shen piperazine mycin 30%, epoxiconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, OPEO sodium sulfonate 3%, 1,2-PD 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 30.5% Shen piperazine mycin epoxiconazole suspension concentrate can be obtained.
Comparative example 8:30.5% Shen piperazine mycin hexaconazole suspending agent (60:1)
Component: Shen piperazine mycin 30%, own azoles alcohol 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, OPEO sodium sulfonate 3%, 1,2-PD 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: the same.
Two: Shen piperazine mycin and Flusilazole composite
Embodiment 10:30.5% Shen piperazine mycin Flusilazole wetting powder (1:60)
Component: Shen piperazine mycin 0.5%, Flusilazole 30%, neopelex 4%, lignin sodium naphthalene sulfonate 12%, white carbon 20%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 30.5% Shen piperazine mycin Flusilazole wetting powder.
Comparative example 9:30.5% polyoxin Flusilazole wetting powder (1:60)
Component: polyoxin 0.5%, Flusilazole 30%, neopelex 4%, lignin sodium naphthalene sulfonate 12%, white carbon 20%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 11:31% Shen piperazine mycin Flusilazole wetting powder (1:30)
Component: Shen piperazine mycin 1%, Flusilazole 30%, neopelex 3%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 12%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 31% Shen piperazine mycin Flusilazole wetting powder.
Comparative example 10:31% Shen piperazine mycin Tebuconazole wetting powder (1:30)
Component: Shen piperazine mycin 1%, Tebuconazole 30%, neopelex 3%, naphthalenesulfonic acid-formaldehyde condensate 8%, white carbon 12%, talcum powder complements to 100%.
Preparation method: the same.
Embodiment 12:16% Shen piperazine mycin Flusilazole dry suspending agent (1:15)
Component: Shen piperazine mycin 1%, Flusilazole 15%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine mycin Flusilazole dry suspending agent.
Comparative example 11:16% polyoxin Flusilazole dry suspending agent (1:15)
Component: polyoxin 1%, Flusilazole 15%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the same.
Embodiment 13:20% Shen piperazine mycin flusilazole water dispersible granule (1:1)
Component: Shen piperazine mycin 10%, Flusilazole 10%, lauryl sodium sulfate 4%, polycarboxylic acids sodium 5%, anhydrous sodium sulfate 15%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 20% Shen piperazine mycin flusilazole water dispersible granule.
Comparative example 12:20% Shen piperazine mycin difenoconazole water dispersible granule (1:1)
Component: Shen piperazine mycin 10%, Difenoconazole 10%, lauryl sodium sulfate 4%, polycarboxylic acids sodium 5%, anhydrous sodium sulfate 15%, kaolin complements to 100%.
Preparation method: the same.
Embodiment 14:21% Shen piperazine mycin Flusilazole wetting powder (19:2)
Component: Shen piperazine mycin 19%, Flusilazole 2%, neopelex 3%, lignin sodium naphthalene sulfonate 8%, white carbon 8%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 21% Shen piperazine mycin Flusilazole wetting powder.
Comparative example 13:21% Shen piperazine mycin prothioconazoles wetting powder (19:2)
Component: Shen piperazine mycin 19%, prothioconazoles 2%, neopelex 3%, lignin sodium naphthalene sulfonate 8%, white carbon 8%, talcum powder complements to 100%.
Preparation method: the same.
Embodiment 15:30.5% Shen piperazine mycin Flusilazole suspending agent (60:1)
Component: Shen piperazine mycin 30%, Flusilazole 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 30.5% Shen piperazine mycin Flusilazole suspending agent can be obtained.
Comparative example 14:30.5% Shen piperazine mycin tebuconazole suspension concentrates (60:1)
Component: Shen piperazine mycin 30%, Tebuconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: the same.
Three: Shen piperazine mycin and tetraconazole composite
Embodiment 16:30.5% Shen piperazine mycin tetraconazole wetting powder (1:60)
Component: Shen piperazine mycin 0.5%, tetraconazole 30%, neopelex 6%, lignin sodium naphthalene sulfonate 12%, white carbon 25%, talcum powder complements to 100%.
Preparation method: by tetraconazole and white carbon mixing and absorption, then other each components are added mix, through mechanical crushing, then after air-flow crushing, mix, 30.5% Shen piperazine mycin tetraconazole wetting powder can be obtained.
Comparative example 15:30.5% polyoxin tetraconazole wetting powder (1:60)
Component: polyoxin 0.5%, tetraconazole 30%, neopelex 6%, lignin sodium naphthalene sulfonate 12%, white carbon 25%, talcum powder complements to 100%.
Preparation method: the same.
Embodiment 17:31% Shen piperazine mycin tetraconazole wetting powder (1:30)
Component: Shen piperazine mycin 1%, tetraconazole 30%, lauryl sodium sulfate 6%, lignin sodium naphthalene sulfonate 12%, white carbon 25%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.
Preparation method: by tetraconazole and white carbon mixing and absorption, then other each components are added mix, through mechanical crushing, then after air-flow crushing, mix, 31% Shen piperazine mycin tetraconazole wetting powder can be obtained.
Comparative example 16:31% Shen piperazine mycin prothioconazoles wetting powder (1:30)
Component: Shen piperazine mycin 1%, prothioconazoles 30%, lauryl sodium sulfate 6%, lignin sodium naphthalene sulfonate 12%, white carbon 25%, anhydrous sodium sulfate 8%, talcum powder complements to 100%.
Preparation method: the same.
Embodiment 18:20% Shen piperazine mycin tetraconazole wetting powder (1:1)
Component: Shen piperazine mycin 10%, tetraconazole 10%, neopelex 3%, dodecyl naphthalenesulfonate formaldehyde condensation compound 8%, white carbon 12%, precipitated calcium carbonate complements to 100%.
Preparation method: preparation method: by tetraconazole and white carbon mixing and absorption, then other each components are added mix, through mechanical crushing, then after air-flow crushing, mix, 20% Shen piperazine mycin tetraconazole wetting powder can be obtained.
Comparative example 17:20% polyoxin tetraconazole wetting powder (1:1)
Component: polyoxin 10%, tetraconazole 10%, neopelex 3%, dodecyl naphthalenesulfonate formaldehyde condensation compound 8%, white carbon 12%, precipitated calcium carbonate complements to 100%.
Preparation method: the same.
Embodiment 19:16% Shen piperazine mycin tetraconazole suspending agent (15:1)
Component: Shen piperazine mycin 15%, tetraconazole 1%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, OPEO sodium sulfonate 3%, methyl oleate 10%, 1,2-PD 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin tetraconazole suspending agent can be obtained.
Comparative example 18:16% Shen piperazine mycin prothioconazoles suspending agent (15:1)
Component: Shen piperazine mycin 15%, tetraconazole 1%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, OPEO sodium sulfonate 3%, methyl oleate 10%, 1,2-PD 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: the same.
Embodiment 20:31% Shen piperazine mycin tetraconazole dry suspending agent (30:1)
Component: Shen piperazine mycin 30%, tetraconazole 1%, sodium lignin sulfonate 10%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 31% Shen piperazine mycin tetraconazole dry suspending agent.
Comparative example 19:31% polyoxin tetraconazole dry suspending agent (30:1)
Component: polyoxin 30%, tetraconazole 1%, sodium lignin sulfonate 10%, lignin sodium naphthalene sulfonate 10%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, white carbon 10%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the same.
Embodiment 21:30.5% Shen piperazine mycin tetraconazole suspending agent (60:1)
Component: Shen piperazine mycin 30%, tetraconazole 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 30.5% Shen piperazine mycin tetraconazole suspending agent can be obtained.
Comparative example 20:30.5% Shen piperazine mycin prothioconazoles suspending agent (60:1)
Component: Shen piperazine mycin 30%, prothioconazoles 0.5%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO sodium sulfonate 4%, ethylene glycol 6%, XG 0.25%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: the same.
Find through experiment, the epoxiconazole in above embodiment, Flusilazole, tetraconazole can be replaced mutually, to form new embodiment.No longer carry out this repeating to enumerate, it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Sigatoka, wheat leaf embroidery disease is prevented and treated with the pesticidal preparations that above-described embodiment is obtained.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 12.5% epoxiconazole SC, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m
2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: the diagonal sampling of every community, often clicks 10 strains.Carry out classification with the bacterium ball number on every strain grain, result of the test sees the following form 4,5:
Sigatoka investigation grade scale:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area;
Wheat leaf embroiders sick investigation grade scale:
Wheat leaf is embroidered disease field investigation standard and is adopted following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-25% of whole leaf area;
5 grades: lesion area accounts for the 26%-50% of whole leaf area;
7 grades: lesion area accounts for the 51%-75% of whole leaf area;
9 grades: lesion area accounts for more than 76% of whole leaf area;
Table 4 Shen piperazine mycin and the composite control efficiency to sigatoka of epoxiconazole
Table 4 field efficacy result shows, within the scope of weight ratio 1:60-60:1, Shen piperazine mycin and epoxiconazole Complex synergism obvious, particularly within the scope of 1:30-30:1, especially within the scope of 1:15-15:1, have excellent control efficiency to banana tikka, control efficiency is better than single dose kind.
Table 5 embodiment is embroidered sick control efficiency with comparative example to wheat leaf and is compared
Inventor find other antibiotics bactericide and epoxiconazole composite, Shen piperazine mycin and other triazole bactericidal agents composite, within the scope of weight ratio 1:60-60:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational jinggangmeisu of most, polyoxin, propiconazole, own azoles alcohol constructs comparative example and embodiment carries out preventive effect contrast, as table 5 field efficacy result shows, (particularly 1:30-30:1 within the scope of weight ratio 1:60-60:1, especially 1:15-15:1) Shen piperazine mycin and epoxiconazole Complex synergism obvious, to the control efficiency of plant disease, be better than other antibiotics bactericide and the composite control efficiency of epoxiconazole, be also better than the composite effect of Shen piperazine mycin and other triazole bactericidal agents.
The field trial of scab of cucumber, wheat powdery mildew is prevented and treated with the pesticidal preparations that above-described embodiment obtains.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 400g/l Flusilazole, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m
2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: the diagonal sampling of every community, often clicks 10 strains.Carry out classification with the bacterium ball number on every strain grain, result of the test sees the following form 6,7:
Scab of cucumber investigation grade scale:
0 grade: asymptomatic
1 grade: visible water soaking mode chlorisis point or yellow point appear in blade;
3 grades: the withered spot of minority appears in blade, and withered spot accounts for blade area less than 10%;
5 grades: the withered spot of blade accounts for blade area 11%-30%, and withered spot appears in petiole;
7 grades: the withered spot of blade accounts for blade area 31%-60% or depression spot appears in petiole, petiole cracking sometimes;
9 grades: the withered spot of blade accounts for blade area more than 61% or petiole fractures.
Wheat powdery mildew investigation grade scale:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for less than 50% of whole leaf area;
Table 6 Shen piperazine mycin and the composite control efficiency to scab of cucumber of Flusilazole
Table 6 field efficacy result shows, within the scope of weight ratio 1:60-60:1, Shen piperazine mycin and Flusilazole Complex synergism obvious, particularly within the scope of 1:30-30:1, especially within the scope of 1:15-15:1, have excellent control efficiency to scab of cucumber, control efficiency is better than single dose kind.
Table 7 embodiment compares with the control efficiency of comparative example to wheat powdery mildew
Inventor find other antibiotics bactericide and Flusilazole composite, Shen piperazine mycin and other triazole bactericidal agents composite, within the scope of weight ratio 1:60-60:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational polyoxin of most, propiconazole, Tebuconazole, Difenoconazole, prothioconazoles constructs comparative example and embodiment carries out preventive effect contrast, as table 7 field efficacy result shows, (particularly weight ratio 1:30-30:1 within the scope of weight ratio 1:60-60:1, within the scope of weight ratio 1:15-15:1), Shen piperazine mycin and Flusilazole Complex synergism obvious, to the control efficiency of plant disease, be better than other antibiotics bactericide and the composite control efficiency of Flusilazole, also Shen piperazine mycin and the composite control efficiency of other triazole bactericidal agents is better than.
The pesticidal preparations control powdery mildew of strawberry obtained with above-described embodiment, the test of Hami melon powdery mildew.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 12.5% tetraconazole aqueous emulsion, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m
2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: the diagonal sampling of every community, often clicks 10 strains.Carry out classification with the bacterium ball number on every strain grain, result of the test sees the following form 8,9:
Powdery mildew of strawberry, Hami melon powdery mildew pilot survey grade scale:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for less than 50% of whole leaf area;
Table 8 Shen piperazine mycin and the composite control efficiency to powdery mildew of strawberry of tetraconazole
Table 8 field efficacy result shows, within the scope of weight ratio 1:60-60:1, Shen piperazine mycin and tetraconazole Complex synergism obvious, particularly within the scope of 1:30-30:1, especially within the scope of 1:15-15:1, have excellent control efficiency to powdery mildew of strawberry, control efficiency is better than single dose kind.
Table 9 embodiment compares with the control efficiency of comparative example to Hami melon powdery mildew
Inventor find other antibiotics bactericide and tetraconazole composite, Shen piperazine mycin and other triazole bactericidal agents composite, within the scope of weight ratio 1:60-60:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational polyoxin of most, prothioconazoles constructs comparative example and embodiment carries out preventive effect contrast, as table 9 field efficacy result shows, within the scope of weight ratio 1:60-60:1, Shen piperazine mycin and tetraconazole Complex synergism are obvious, to the control efficiency of plant disease, be better than other antibiotics bactericide and the composite control efficiency of tetraconazole, be also better than the composite effect of Shen piperazine mycin and prothioconazoles.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a bactericidal composition, is characterized in that, active ingredient contains Shen piperazine mycin and triazole bactericidal agent, and the weight ratio of described Shen piperazine mycin and triazole bactericidal agent is 1:60 ~ 60:1.
2. bactericidal composition according to claim 1, is characterized in that, the weight ratio of described Shen piperazine mycin and triazole bactericidal agent is 1:30 ~ 30:1.
3. bactericidal composition according to claim 2, is characterized in that, the weight ratio of described Shen piperazine mycin and triazole bactericidal agent is 1:15 ~ 15:1.
4., according to described bactericidal composition arbitrary in claims 1 to 3, it is characterized in that, described triazole bactericidal agent is epoxiconazole, Flusilazole or tetraconazole.
5. according to described bactericidal composition arbitrary in Claims 1-4, it is characterized in that, also containing pesticidal preparations auxiliary element in described bactericidal composition, to make the formulation being applicable to agriculturally using.
6. bactericidal composition according to claim 5, is characterized in that, described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent; The formulation of described bactericidal composition is suspending agent, dry suspending agent, water dispersible granules or wetting powder.
7. the application of the arbitrary described bactericidal composition of claim 1 to 6 in the pesticidal preparations of preparation controlling plant diseases.
8. the application of bactericidal composition according to claim 7, is characterized in that, the application in the pesticidal preparations preparing the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, banana freckle, banana black disease disease or graw mold of tomato.
9. the application method of the bactericidal composition described in claim 1 to 6, is characterized in that, active principle Shen piperazine mycin and triazole bactericidal agent are used with weight ratio 1:60 ~ 60:1, and it is used after mixing in proportion, use in proportion simultaneously or use successively in proportion.
10. the application method of bactericidal composition according to claim 9, it is characterized in that, active principle Shen piperazine mycin and triazole bactericidal agent are used with weight ratio 1:30 ~ 30:1, and it is used after mixing in proportion, use in proportion simultaneously or use successively in proportion.
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| CN108651475A (en) * | 2018-06-24 | 2018-10-16 | 合肥红佳科技信息有限公司 | A kind of bactericidal composition containing shenqinmycin and triticonazole |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101705265A (en) * | 2009-11-12 | 2010-05-12 | 上海交通大学 | Method for producing phenazine-1-carboxylic acid by using engineering bacterial strain M18G to carry plasmid pME6032Phz |
| CN102249799A (en) * | 2011-04-27 | 2011-11-23 | 上海农安生物科技发展有限公司 | Method for controlling crop diseases with shenqinmycin and shenqinmycin preparation |
| CN103155926A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Sterilization composite based on phenazino-1-carboxylicacid |
| CN103444477A (en) * | 2013-09-18 | 2013-12-18 | 吴洁远 | Super rice strengthening and harm control cultivation method based on prevention and control of diseases, insects, lodging and pollution |
| CN103875494A (en) * | 2014-04-14 | 2014-06-25 | 吴洁远 | Standardized cultivation method of super double cropping rice |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3511535B2 (en) * | 1995-03-20 | 2004-03-29 | 住友林業株式会社 | Materials and methods for controlling snow rot |
| WO1998024773A1 (en) * | 1996-12-03 | 1998-06-11 | Nippon Chemiphar Co., Ltd. | Phenazine derivatives |
| US6150363A (en) * | 1996-12-03 | 2000-11-21 | Nippon Chemiphar Co., Ltd. | Dihydrophenazinecarboxylic acid derivatives |
| US6348193B1 (en) * | 1999-09-02 | 2002-02-19 | Board Of Trustees Of Michigan State University | Method for inhibiting pathogenic fungi and bacteria in plants using Pseudomonas aureofaciens |
-
2015
- 2015-06-01 CN CN201510292410.2A patent/CN104957145B/en active Active
- 2015-06-01 CN CN201510290406.2A patent/CN104957152B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101705265A (en) * | 2009-11-12 | 2010-05-12 | 上海交通大学 | Method for producing phenazine-1-carboxylic acid by using engineering bacterial strain M18G to carry plasmid pME6032Phz |
| CN102249799A (en) * | 2011-04-27 | 2011-11-23 | 上海农安生物科技发展有限公司 | Method for controlling crop diseases with shenqinmycin and shenqinmycin preparation |
| CN103155926A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Sterilization composite based on phenazino-1-carboxylicacid |
| CN103444477A (en) * | 2013-09-18 | 2013-12-18 | 吴洁远 | Super rice strengthening and harm control cultivation method based on prevention and control of diseases, insects, lodging and pollution |
| CN103875494A (en) * | 2014-04-14 | 2014-06-25 | 吴洁远 | Standardized cultivation method of super double cropping rice |
Non-Patent Citations (2)
| Title |
|---|
| 张穗等: ""申嗪霉素对小麦纹枯病菌的毒力测定及盆栽药效"", 《上海交通大学学报》 * |
| 金啸等: ""四种杀菌剂对两种赤眼蜂的毒性分析及敏感性比较"", 《农药学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719695A (en) * | 2017-04-09 | 2017-05-31 | 佛山市瑞生通科技有限公司 | A kind of bactericidal composition of the mycin of second containing fen and tetraconazole |
| CN108651475A (en) * | 2018-06-24 | 2018-10-16 | 合肥红佳科技信息有限公司 | A kind of bactericidal composition containing shenqinmycin and triticonazole |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104957152A (en) | 2015-10-07 |
| CN104957145B (en) | 2017-04-05 |
| CN104957152B (en) | 2017-03-15 |
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