CN102835399B - Metrafenone-containing bactericidal composition - Google Patents
Metrafenone-containing bactericidal composition Download PDFInfo
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Abstract
The invention discloses a metrafenone-containing bactericidal composition, which consists of a first active ingredient and a second active ingredient, wherein the first active ingredient is metrafenone; the second active ingredient is strobilurin fungicide which is selected from azoxystrobin, (E)-2-[2-(2, 5-dimethyl phenoxyl toluene)-benzyl]-3-methoxy methyl acrylate and coumoxystrobin; and the weight ratio of the first active ingredient to the second active ingredient is 1:10-20:1, preferably 1:5-10:1. The composition can be prepared into agriculturally permissible formulations such as water dispersible granule, wettable powder, suspension and dispersible oil suspension. The metrafenone-containing bactericidal composition has the advantages of rational composition, good bactericidal effect and low medication cost; the activity and the bactericidal effect of the metrafenone-containing bactericidal composition are not simple superposition of activity of each component; and compared with the conventional single preparation, the metrafenone-containing bactericidal composition has an obvious bactericidal effect and an obvious synergistic effect, is pollution-free for crops, has high safety, meets the safety requirements of agricultural preparations and has an obvious prevention and treatment effect on powdery mildew, downy mildew, epidemic diseases and anthracnose of the crops.
Description
Technical field
The present invention relates to a kind of bactericidal composition containing metrafenone, particularly a kind of bactericidal composition being comprised of metrafenone and methoxy acrylic bactericide, belongs to technical field of compounded pesticide.
Background technology
Metrafenone; English common name: Metrafenone; chemical name: (the bromo-6-methoxyl group-2-of 3-aminomethyl phenyl)-(2; 3; 4-trimethoxy-6-aminomethyl phenyl) ketone; be the agricultural fungicidal agent of being developed by BASF AG, to all kinds of crop powdery mildews, have protection, treatment and inhibition to produce the effect of spore.
Methoxy acrylic acid series bactericidal agent is in recent years bactericide kind newly developed, is a large milestone in bactericide field.Methoxy acrylic bactericide has unique mechanism of action, the active group of this series bactericidal agent is methoxy acrylic acid (ester/acid amides), the cytochrome b el compound of Main Function in the mitochondrial respiratory chain of fungi, thus the growth of electronics transmission Antifungi stoped.There is protection, treat, root out, infiltration, systemic activity, and can be in plant corpus, degraded very soon in soil and water.Although this series bactericidal agent mechanism of action uniqueness, the same with other bactericide, methoxy acrylic bactericide is also unable to escape the misfortune of resistance, and use cost is high, so limited the spread of methoxy acrylic acid series bactericidal agent, uses.
Introduce several methoxy acrylic acid series bactericidal agent kinds below.
Fluoxastrobin, other Chinese name: Amici reaches, English common name: azoxystrobin, chemical name: (E) 2-[2-[6-(2-cyano-benzene oxygen) pyrimidine-4-yl oxygen] phenyl]-3-methoxy-methyl acrylate.Efficiently, wide spectrum; almost can prevent and treat all fungal diseases; powdery mildew, rust, glume blight, net blotch, downy mildew, rice blast etc. are all had to good activity, there is protection, root out, infiltration and systemic action, can suppress the sprouting of spore and the growth of mycelia.Can be used for cauline leaf processing, seed treatment, also can carry out soil treatment.Suitable crop is cereal, paddy rice, grape, potato, vegetables, fruit tree, beans and other crop etc.
ZJ0712; chemical name: (E)-2-[2-(2; 5-dimethyl phenoxy toluene)-phenyl]-3-methoxy-methyl acrylate; ZJ0712 is wide spectrum, the systemic fungicide of being developed by Zhejiang chemical research institute; bactericidal activity is higher; have protection and therapeutic action concurrently, can be used for preventing and treating the diseases such as powdery mildew, downy mildew, anthracnose.
SYP-3375, English common name: coumoxystrobin, chemical name: (E)-2-(2-((3-butyl-4-methyl-coumarin-7-Ji-oxygen base) methyl) phenyl)-3-methoxy-methyl acrylate.There is protection and therapeutic action; be a kind of disinfectant use in agriculture of high-efficiency broad spectrum, the plant disease causing by mastigomycetes, in conjunction with bacterium, sac fungi, basidiomycetes and imperfect fungus is had to good preventive effect as sclerotinia rot of colza, apple valsa canker, rice blast, sigatoka, alternaria leaf spot of apple etc.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, the agricultural bactericidal composition to crop safety.
Another object of the present invention is to provide the application of above-mentioned composition in control crop powdery mildew, downy mildew, epidemic disease, anthracnose.
In order to overcome the defect of existing unitary agent, technical scheme of the present invention is to solve like this:
Containing a bactericidal composition for metrafenone, by the first active component and the second active component, formed, wherein,
(1) first active component is metrafenone;
(2) second active components are methoxy acrylic bactericide, and described methoxy acrylic bactericide is preferably from Fluoxastrobin, ZJ0712, SYP-3375;
The weight ratio of the first active component and the second active component is 1:10～20:1, is preferably 1:5～10:1, and more preferably 1:2～5:1, most preferably is 2:1.
The first active component and the second active component content sum are described composition total weight 5%～85%, are preferably 8%～70%.
The preparation formulation that bactericidal composition of the present invention can be prepared according to method known in those skilled in the art is water dispersible granules, wetting powder, suspending agent, dispersibles oil-suspending agent.
For water dispersible granules, those skilled in the art are familiar with the corresponding auxiliary agent of use and complete the present invention very much.Dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Disintegrant as in ammonium sulfate, urea, sucrose, glucose one or more; Binding agent is as one or more in diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family; Filler as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To wetting powder, spendable auxiliary agent has: dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Filler as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To suspending agent, spendable auxiliary agent has: dispersant is produced as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), TERSPERSE 2020(U.S. Huntsman Corporation, alkyl naphthalene sulfonic acid salt) in one or more; Emulsifier is as Nongru-700 #(common name: alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this dish-60#(common name: Span60), Tween-60 #(common name: polyoxyethylene sorbitan stearate), agriculture breast 1601#(common name: triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), one or more in TERSPERSE 4894; Wetting agent as in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 one or more; Thickener as in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate one or more; Preservative as in formaldehyde, benzoic acid, Sodium Benzoate one or more; Defoamer is as organic silicon defoamer; Antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more.
To dispersibling oil-suspending agent, spendable auxiliary agent has: dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, TERSPERSE 2020 one or more; Emulsifier is as BY(castor oil polyoxyethylene ether) one or more in serial emulsifier (BY-110, BY-125, BY-140), Nongru-700 #, agriculture breast 2201#, this dish-60#, Tween-60 #, agriculture newborn 1601#, TERSPERSE 4894; Wetting agent as in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 one or more; Thickener as in white carbon, polyvinyl alcohol, bentonite, aluminium-magnesium silicate one or more; Antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more; Dispersion medium as in soybean oil, rapeseed oil, corn oil, methyl oleate, diesel oil, machine oil, mineral oil one or more.
Embodiment
Below by Toxicity Determination, metrafenone and Fluoxastrobin, ZJ0712, the synergistic effect of SYP-3375 mixture to powdery mildew, downy mildew, epidemic disease, anthracnose are described.
In order to prevent and treat crop powdery mildew, inventor has carried out mutual composite synergy research with metrafenone and Fluoxastrobin, ZJ0712, SYP-3375, and concrete grammar is:
Subjects is powdery mildew of cucumber bacterium sphaerotheca fuliginea.Former medicine is mixed with to the test medicine needing, test method is with reference to < < People's Republic of China's agricultural industry criteria NY/T1156.11-2008 farm-chemical indoor determination test rule bactericide the 11st part: control cucurbits powdery mildew test pot-culture method > >.First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different medicament combinations, concentration gradient setting is also different, and preventive effect is set by geometric progression in 5%～90% scope).With the pure water that is added with a small amount of surfactant (Tween 80), wash and get the fresh spore covering with on Powdery Mildew cucumber leaves, with double gauze filtration, making spore concentration is 1 × 10
5the suspension of individual spore/milliliter is standby.Every processing 3 basins, repeat for 4 times, and only establish the processing that does not contain active ingredient containing solvent and surfactant and make blank.With spore suspension 24h inoculation before chemicals treatment, liquid is evenly sprayed in blade face to all wetting, standby after liquid natural air drying.Potted plant cucumber natural air drying after inoculation and chemicals treatment, then moves to thermostatic chamber, under the condition of 20 ℃～24 ℃ of temperature, cultivates 7 days～10 days.Until the sick leaf rate of blank, reach 80% when above, classification investigation is managed incidence everywhere, and 30 leaves are at least investigated in every processing, and grade scale is:
0 grade: anosis;
1 grade: lesion area accounts for below 5% of one-piece blade area;
3 grades: lesion area accounts for 6%～15% of one-piece blade area;
5 grades: lesion area accounts for 16%～25% of one-piece blade area;
7 grades: lesion area accounts for 26%～50% of one-piece blade area;
9 grades: lesion area accounts for 50%～75% of one-piece blade area;
11 grades: lesion area accounts for the more than 75% of one-piece blade area.
By the linear regression analysis between the probit value of preventive effect and the logarithm value of series concentration, obtain the EC of each medicament
50value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: metrafenone has good virulence as 1:10～20:1 is mixed to powdery mildew of cucumber take weight ratio with Fluoxastrobin, ZJ0712 or SYP-3375, all have good synergistic effect, especially take weight ratio as 1:5～10:1 synergy the most remarkable.
In order to prevent and treat the downy mildew in agricultural production, inventor has carried out mutual composite synergy research with metrafenone and Fluoxastrobin, ZJ0712 or SYP-3375, and concrete grammar is:
It is tested object that test adopts bacterium of downy mildew of cucumber (Pseudoperonospora cubensis).Test method is with reference to < < People's Republic of China's agricultural industry criteria NY/T1156.3-2006 > >.First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different medicament combinations, concentration gradient setting is also different, and bacteriostasis rate is set in 5%～90% scope).Test target bacterium is to pick up from the from up to down bacterium of downy mildew of cucumber blade of 4 leaf positions, leaf～6 of field, with the Pseudoperonospora cubensis sporangium at 4 ℃ of distillation washing lower blade back sides, be made into suspension (concentration is controlled at every milliliter of 1 × 105～1 × 107 sporangium), deposit at 4 ℃ standby; Liquid is evenly sprayed in the healthy leaves back side, after liquid natural air drying, each processing blade and blade is gone up dorsad, by being emitted in moisture preservation box after marks for treatment.Test is established the not processing containing medicament and is made blank.Be inoculated in vacuum side of blade with ready fresh sporangia suspension drop 10 μ L.4 of every blade inoculations, every processing is no less than 5 leaves.24h inoculation before processing.Ware lid on inoculation bonnet, is placed in climatic cabinate or has the humidistat of illumination, and alternately, temperature is 17 ℃～22 ℃ to the continuous illumination in every day/dark 12h, under more than 90% condition of relative moisture, cultivates.Depending on processing and blank incidence, measure and record scab diameter, unit is millimeter (mm), calculates control efficiency.By the linear regression analysis between the probit value of control efficiency and the logarithm value of series concentration, obtain the EC of each medicament
50value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: metrafenone has good virulence as 1:10～20:1 is mixed to cucumber downy mildew take weight ratio with Fluoxastrobin, ZJ0712 or SYP-3375, all have good synergistic effect, especially take weight ratio as 1:5～10:1 synergy the most remarkable.
In order to prevent and treat epidemic disease, the anthracnose in agricultural production, inventor has carried out mutual composite synergy research with metrafenone and Fluoxastrobin, ZJ0712, SYP-3375, and concrete grammar is:
It is tested object that test adopts tomato early blight bacterium (Alternaria solani), apple anthrax bacteria (Gleosporium fructigenum Berk).Former medicine is mixed with to the test medicine needing, test method is with reference to < < People's Republic of China's agricultural industry criteria NY/T1156.2-2006 > >.First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different medicament combinations, concentration setting is also different, and bacteriostasis rate is set in 5%～90% scope).If clear water contrast, repeats 3 times.Adopt mycelial growth rate method, by cultured pathogen, be 5mm card punch with diameter under aseptic condition, from colony edge, cuts bacterium cake, bacterium cake is connected on the culture medium flat plate of different drug concentrations with transfer needle, puts in 26 ℃ of incubators and cultivate 72h; Check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC of each medicament
50value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: metrafenone has good virulence as 1:10～20:1 is mixed to early blight of tomato, apple anthracnose take weight ratio with Fluoxastrobin, ZJ0712 or SYP-3375, all have good synergistic effect, especially take weight ratio as 1:5～10:1 synergy the most remarkable.
Below in conjunction with embodiment, content of the present invention is described further.
Example of formulations 1
Take 80% metrafenone, 5% Fluoxastrobin, 2% TERSPERSE 2700(polycarboxylate, U.S. Huntsman Corporation produces), 1% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% Nekal BX (nekal), 2% K-12(lauryl sodium sulfate), 1% carboxymethyl cellulose, 2% urea, diatomite add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 85% metrafenone azoxystrobin water dispersible granule.
Example of formulations 2
Take 5% metrafenone, 50% ZJ0712,3% TERSPERSE 2700,2% dispersing agent NNO, 3% Nekal BX, 2%K-12,3% diatomite, 5% glucose, kaolin and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 55% metrafenone ZJ0712 water dispersible granules.
Example of formulations 3
Take 30% metrafenone, 30% SYP-3375,3% TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 1%K-12,1% carboxymethyl cellulose, 3% urea, diatomite and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 60% metrafenone SYP-3375 water dispersible granules.
Example of formulations 4
Take 50% metrafenone, 2.5% Fluoxastrobin, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% White Carbon black, fine particle calcium carbonate and add to 100% weight portion.Above-mentioned raw materials, through mixing, makes 52.5% metrafenone Fluoxastrobin wetting powder after air-flow crushing.
Example of formulations 5
Take 15% metrafenone, 30% ZJ0712,4% Morwet D-425(alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO, 2% Nekal BX, 2%K-12,3% White Carbon black, kaolin add to 100% weight portion.Above-mentioned raw materials, through mixing, makes 45% metrafenone ZJ0712 wetting powder after air-flow crushing.
Example of formulations 6
Take 35% metrafenone, 7% SYP-3375,5% calcium lignosulfonate, 2% Nekal BX, 2%K-12,5% White Carbon black, kaolin and add to 100% weight portion.Above-mentioned raw materials, through mixing, makes 42% metrafenone SYP-3375 wetting powder after air-flow crushing.
Example of formulations 7
Take 21% metrafenone, 7% Fluoxastrobin, 4% TERSPERSE 4894(U.S. Huntsman Corporation produces), 1.5% TERSPERSE 2500(U.S. Huntsman Corporation produces), 2% TERSPERSE 2020(U.S. Huntsman Corporation produces), 1% aluminium-magnesium silicate, 0.2% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 28% metrafenone Fluoxastrobin suspending agent after sand mill sand milling.
Example of formulations 8
Take 6% metrafenone, 30% ZJ0712,5%TERSPERSE 4894,2%TERSPERSE 2500,3%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 36% metrafenone ZJ0712 suspending agent after sand mill sand milling.
Example of formulations 9
Take 20% metrafenone, 10% SYP-3375,2%TERSPERSE 2020,2% this dish-60#, 2% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 30% metrafenone SYP-3375 suspending agent after sand mill sand milling.
Example of formulations 10
Take 2% metrafenone, 3% Fluoxastrobin, 2% dispersing agent NNO, 3% agriculture breast 2201#, 10% BY-110,1%TERSPERSE 2500,1% aluminium-magnesium silicate, 3% glycerine, soybean oil adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 5% metrafenone Fluoxastrobin after sand mill sand milling, dispersibles oil-suspending agent.
Example of formulations 11
Take 32% metrafenone, 8% ZJ0712,1.5%TERSPERSE 2020,3% this dish-60#, 3% Tween-60 #, 8% BY-110,1%TERSPERSE 2500,0.5% white carbon, 5% propane diols, methyl oleate and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 40% metrafenone ZJ0712 after sand mill sand milling, dispersibles oil-suspending agent.
Example of formulations 12
Take 6% metrafenone, 18% SYP-3375,1% dispersing agent NNO, 5% BY-125,3% this dish-60#, 3% Tween-60 #, 4% agriculture breast 1601#, 1% phenethyl phenol polyethenoxy base ether phosphate, 0.5% bentonite, 5% glycerine, rapeseed oil adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 24% metrafenone SYP-3375 after sand mill sand milling, dispersibles oil-suspending agent.
Biological Examples 1: control wheat powdery mildew field trial
Within 2012, at Shaanxi Province's Ankang City, carried out example of formulations 7(28% metrafenone Fluoxastrobin suspending agent), example of formulations 5(45% metrafenone ZJ0712 wetting powder), example of formulations 3(60% metrafenone SYP-3375 water dispersible granules) control wheat powdery mildew field trial, verified the control efficiency of these several medicaments to wheat powdery mildew and the safety to wheat.Trial crops is wheat, and controlling object is wheat powdery mildew (Blumeria graminis).Test is located at Ankang City Hanyin County, and experimental field physical features is smooth, and soil is loam, and fertility is better-than-average, pH value 7.0, and duration of test rich water quality management is medium.Test medicine and dosage refer to table 5.Separately establish blank, every processing repeats for 4 times, every community 33 ㎡, and Gong32Ge community, random district group is arranged.Adopt conventional spray-on process, mu dispenser liquid 45kg, is evenly sprayed at leave dual sides.At wheat booting period and the each spraying of flowering initial stage, prevent and treat 1 time dispenser 2 times altogether.
Investigation and statistical method, the 7th day and the 14th day each investigation 1 time after the 2nd dispenser, investigate 2 times altogether.Adopt random five point samplings, get 20 strains at every, investigate upper 3 leaves (having boot leaf to comprise boot leaf) of every strain, record total number of sheets, the sick number of sheets, sick leaf rate, according to 9 grades of grade scales of wheat powdery mildew, record the sick numbers of sheets at different levels, calculate disease index and preventive effect.After each spray medicine after the 1st day and medicine some days, observe medicament and respectively process the impact on fruit and blade.
Wheat powdery mildew grade scale:
0 grade: without scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for the 6%-15% of whole leaf area;
5 grades: lesion area accounts for the 16%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for the more than 51% of whole leaf area.
According to recording incidence, calculate disease index and control efficiency.
Disease index=[∑ (the sick number of sheets × relative level numerical value at different levels)/(investigation total leaf number × 9)] × 100
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] × 100.
Field test results shows, 28% metrafenone Fluoxastrobin suspending agent, 45% metrafenone ZJ0712 wetting powder, 60% metrafenone SYP-3375 water dispersible granules has good control efficiency to wheat powdery mildew, press respectively 10g/667 ㎡, 10g/667 ㎡, 6g/667 ㎡ consumption, to wheat powdery mildew for the second time after medicine the preventive effect of 7 days be respectively 89.6%, 90.9%, 93.5%, the preventive effect of the 14th day is respectively 86.5%, 87.4%, 85.1%, obviously be better than 20% metrafenone suspending agent 20g/667 ㎡, 25% Fluoxastrobin suspending agent 50g/667 ㎡, 10% ZJ0712 suspending agent 60g/667 ㎡, the preventive effect of 20% SYP-3375 suspending agent 30g/667 ㎡, difference is (in Table 5) extremely significantly.
To the security survey of wheat, within after spray medicine after the 1st day and medicine some days, to observe, each test is processed wheat leaf blade, flower, fringe is occurred without poisoning phenomenon.
Result of the test shows after metrafenone and Fluoxastrobin, ZJ0712, SYP-3375 mixture, obviously improved the control efficiency to wheat powdery mildew, synergistic effect is obvious, has reduced dosage and drug cost, to wheat growth safety, it is the desirable medicament of control wheat powdery mildew.
Biological Examples 2: control cucumber downy mildew field trial
Within 2012, in Baiyun District, Guangzhou City, carried out example of formulations 7(28% metrafenone Fluoxastrobin suspending agent), example of formulations 11(40% metrafenone ZJ0712 dispersibles oil-suspending agent), example of formulations 9(30% metrafenone SYP-3375 suspending agent) control cucumber downy mildew field trial, verified the control efficiency of these several medicaments to cucumber downy mildew and the safety to cucumber.Test method is with reference to < < pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating cucumber downy mildew GB/T 17980.26-2000 > >.Trial crops is cucumber, and controlling object is cucumber downy mildew (Pseudoperonospora cubensis).Test is located at Taihe county town, Baiyun District, soil pH value 6.5, and fertility is better, the content of organic matter 1.52%, experimental field cultivation condition uniformity.Test medicine and dosage refer to table 6.Separately establish blank, every processing repeats for 4 times, every community area 45 ㎡, and Gong32Ge community, random district group is arranged.Adopt conventional spray-on process, mu dispenser liquid 60kg.During dispenser for the first time, cucumber, in coming into bloom, is total to dispenser 2 times, 7 days, interval.
Investigation and statistical method:
After medicine for the first time, after 7 days and dispenser for the second time, within 14 days, carry out disease index investigation, 5 points are investigated in each community, and every some investigation 5 strain cucumber, record total number of sheets, the sick number of sheets at different levels and sick progression, calculate disease index.
Grade scale is:
0 grade, without scab;
1 grade, lesion area accounts for below 5% of whole leaf area;
3 grades, lesion area accounts for the 6%-10% of whole leaf area;
5 grades, lesion area accounts for the 11%-20% of whole leaf area;
7 grades, lesion area accounts for the 21%-50% of whole leaf area;
9 grades, lesion area accounts for the more than 50% of whole leaf area.
Disease index and control efficiency computational methods:
The total number of sheets × 9 × 100% of disease index=Σ (the sick number of sheets × relative level numerical value at different levels)/investigate
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] × 100
Security survey: spray after medicine the 1st day at every turn and medicine after some days, observe medicament and respectively process the impact on cucumber.
Field test results shows: 28% metrafenone Fluoxastrobin suspending agent 15g/667 ㎡, 40% metrafenone ZJ0712 dispersible oil-suspending agent 10g/667 ㎡, 30% metrafenone SYP-3375 suspending agent 15g/667 ㎡, to cucumber downy mildew for the first time after medicine the preventive effect of 7 days be respectively 78.30%, 76.7%, 77.8%, to cucumber downy mildew for the second time after medicine the preventive effect of 14 days be respectively 87.0%, 84.8%, 83.5%.Obviously be better than 20% metrafenone suspending agent 30g/667 ㎡, 25% Fluoxastrobin suspending agent 50g/667 ㎡, 10% ZJ0712 suspending agent 60g/667 ㎡, the preventive effect (in Table 6) of 20% SYP-3375 suspending agent 30g/667 ㎡ to cucumber downy mildew, shows good quick-acting and lasting effect.
Duration of test is observed, and each chemicals treatment does not produce poisoning phenomenon to cucumber.
Result of the test shows, after metrafenone and Fluoxastrobin, ZJ0712, SYP-3375 mixture, obviously to have improved the control efficiency to cucumber downy mildew, has reduced dosage and drug cost, to cucumber growth safety, is the desirable medicament of control cucumber downy mildew.
From above embodiment, the first active component metrafenone and the second active component Fluoxastrobin, ZJ0712, SYP-3375 combine composite, can be made into water dispersible granules, wetting powder, suspending agent, dispersible oil-suspending agent formulation, powdery mildew of cucumber, wheat powdery mildew, cucumber downy mildew, early blight of tomato, apple anthracnose are had to significant control efficiency, have that dosage is few, control efficiency advantages of higher, saved drug cost and dosage, and good to crop safety.Those skilled in the art can obviously learn that this based composition also can be used as the control of other crop powdery mildew, downy mildew, epidemic disease, anthracnose, therefore produce and have wide practical use.
In sum, composition of the present invention is to adopt two kinds of active component compound schemes, its activity and bactericidal effect are not the simple superposition of each composition activity, compared with existing unitary agent, except having significant bactericidal effect, and there is significant synergistic effect, pollution-free to crop, safety is good, meets the security requirement of pesticidal preparations.
Claims (9)
1. containing a bactericidal composition for metrafenone, it is characterized in that active component is comprised of the first active component and the second active component,
(1) first active component is metrafenone;
(2) second active components are ZJ0712 or SYP-3375;
The weight ratio of the first active component and the second active component is 1:10～20:1.
2. according to claim 1 containing the bactericidal composition of metrafenone, it is characterized in that: the weight ratio of the first active component and the second active component is 1:5～10:1.
3. according to claim 2 containing the bactericidal composition of metrafenone, it is characterized in that: the weight ratio of the first active component and the second active component is 1:2～5:1.
4. according to claim 3 containing the bactericidal composition of metrafenone, it is characterized in that: the weight ratio of the first active component and the second active component is 2:1.
5. according to claim 1 containing the bactericidal composition of metrafenone, it is characterized in that: the first active component and the second active component content sum are described composition total weight 5%～85%.
6. according to claim 5 containing the bactericidal composition of metrafenone, it is characterized in that: the first active component and the second active component content sum are described composition total weight 8%～70%.
7. according to claim 1 containing the bactericidal composition of metrafenone, it is characterized in that: described combination dosage form is water dispersible granules, wetting powder, suspending agent.
8. the application of one of any described bactericidal composition containing metrafenone of claim 1 to 4 in control crop powdery mildew, downy mildew, epidemic disease, anthracnose.
9. application according to claim 8, is characterized in that: the bactericidal composition containing metrafenone is used for preventing and treating powdery mildew of cucumber, wheat powdery mildew, cucumber downy mildew, early blight of tomato, apple anthracnose.
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| CN106259373A (en) * | 2016-08-16 | 2017-01-04 | 李祥英 | A kind of containing fluoxastrobin with the composition pesticide of metrafenone |
| CN108077287A (en) * | 2018-01-12 | 2018-05-29 | 广东广康生化科技股份有限公司 | Bactericidal composition and its application comprising folpet |
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| WO2002067679A1 (en) * | 2001-02-19 | 2002-09-06 | Basf Aktiengesellschaft | Fungicidal mixtures |
| CN1456054A (en) * | 2003-03-25 | 2003-11-19 | 浙江省化工研究院 | Methoxy methyl acrylate compounds as bactericidal agent |
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| WO2002067679A1 (en) * | 2001-02-19 | 2002-09-06 | Basf Aktiengesellschaft | Fungicidal mixtures |
| CN1456054A (en) * | 2003-03-25 | 2003-11-19 | 浙江省化工研究院 | Methoxy methyl acrylate compounds as bactericidal agent |
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| 关爱莹等.杀菌剂丁香菌酯的创制经纬.《农药》.2011,第50卷(第2期), |
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