CN102388890B - Bactericidal composition containing SYP-3375 and cyprodinil - Google Patents
Bactericidal composition containing SYP-3375 and cyprodinil Download PDFInfo
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Abstract
The invention discloses an agricultural bactericidal composition containing SYP-3375 and cyprodinil. The agricultural bactericidal composition comprises effective active ingredients SYP-3375 and cyprodinil, wherein the weight ratio of SYP-3375 to cyprodinil is (1:30)-(10:1). The composition can be prepared into agriculturally allowable dosage forms such as a water dispersible granule, a wettable powder and a suspending agent. The agricultural bactericidal composition disclosed by the invention has reasonable components, good bactericidal effect and low chemical cost, and the activity and the bactericidal effect of the agricultural bactericidal composition are not simple addition of the activities of various components; compared with the existing single preparations, the agricultural bactericidal composition has obvious synergy besides obvious bactericidal effect, thus the generation of resistance is retarded; and the agricultural bactericidal composition has little preparation residue, no pollution to crops and good safety, meets the safety requirement of pesticide preparations and has obvious control efficiency on grape, strawberry, vegetable gray mold, Alternaria mali, rice sheath blight and rice blast.
Description
Technical field
The present invention relates to a kind of bactericidal composition that contains SYP-3375 and cyprodinil, belong to technical field of compounded pesticide.
Background technology
The methoxy acrylic acid series bactericidal agent is in recent years bactericide kind newly developed, is a big milestone in the bactericide field.Methoxy acrylic bactericide has unique mechanism of action, and it stops the ATP of cell synthetic by the electronics transmission of pinning between cytochrome b and the c1, thereby suppresses its mitochondrial respiratory and bring into play bacteriostasis.The bacterial strain that 14-demethylation enzyme inhibitor, benzamides, dicarboxylic dihydrazides amine and benzimidazole is produced resistance is effective.Have protection, treat, root out, infiltration, systemic activity, and can be in plant corpus, very fast degraded in soil and the water.Although this series bactericidal agent mechanism of action uniqueness, but pathogen is also very fast to the speed that it produces resistance, the use cost height, so having limited the large tracts of land of methoxy acrylic acid series bactericidal agent promotes the use of, therefore the bactericide combination of methoxy acrylic acid series bactericidal agent and the another kind of mechanism of action is composite, improve drug effect, slow down the generation of resistance, reduce usage amount, enlarging using of this medicament is present urgent problem.
SYP-3375 is a kind of methoxy-methyl acrylate compounds, English common name: coumoxystrobin, and chemical name: (E)-and 2-(2-((3-butyl-4-methyl-coumarin-7-Ji-oxygen base) methyl) phenyl)-the 3-methoxy-methyl acrylate.Have protection and therapeutic action; be a kind of disinfectant use in agriculture of high-efficiency broad spectrum, the plant disease that causes by mastigomycetes, in conjunction with bacterium, sac fungi, basidiomycetes and imperfect fungus such as sclerotinia rot of colza, apple valsa canker, cucumber downy mildew, rice blast, sigatoka, alternaria leaf spot of apple etc. are had good preventive effect.
Cyprodinil, English common name: Cyprodinil, chemical name: 4-cyclopropyl-6-methyl-N-phenyl pyrimidine-2-amine.Be the methionine biosynthesis inhibitor.Bactericide such as same triazole type, methoxy acrylic acid class do not have cross resistance.Cyprodinil has protection, treatment, blade penetrates and the root systemic activity.Be mainly used in foliar spray or seed treatment, prevent and treat gray mold, powdery mildew, rice blast, scab etc.
Although SYP-3375 is a kind of disinfectant use in agriculture of high-efficiency broad spectrum, it is as methoxy acrylic ester compounds, and the mechanism of action is single, and long-term repeated use germ is gone up in the field application can produce resistance, causes preventive effect to reduce, and the use cost height.Cyprodinil has used for many years, and pathogen has produced very big resistance to it, and it is increasing to use dosage separately.The technology report that does not also have at present SYP-3375 and cyprodinil to be mixed.The inventor finds that by test SYP-3375 and cyprodinil that the mechanism of action is different are composite, have obvious synergistic effect; can significantly improve control efficiency, minimizing spraying times and the dosage for the treatment of, protection; slow down the generation of germ resistance, reduce the control cost, to the crop safety height.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, to the bactericidal composition of crop safety.
Another object of the present invention is to provide the application of above-mentioned composition on control (grape, strawberry, vegetables) gray mold, alternaria leaf spot of apple, rice sheath blight disease, rice blast.
In order to overcome the defective of existing unitary agent, technical scheme of the present invention is to solve like this:
A) the first active component SYP-3375;
B) the second active component cyprodinil;
The weight ratio of first active component and second active component is 1:30～10:1, and preferred weight ratio is 1:10～4:1, and preferred weight ratio is 1:5～2:1.
The cumulant of first active component and second active component is 1%～90% of described composition total weight, is preferably 5%～50%, and the size of active component cumulant is also closely related with formulation, is no more than 50% as the active component cumulant of suspending agent.
It is water dispersible granules, wetting powder, suspending agent that the present invention contains the preparation formulation that the bactericidal composition of SYP-3375 and cyprodinil can prepare according to the known method of those skilled in the art of the present technique.
For water dispersible granules, those skilled in the art are familiar with the corresponding auxiliary agent of use and finish the present invention very much.In dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; In disintegrant such as ammonium sulfate, urea, sucrose, the glucose one or more; In binding agent such as diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family one or more; Filler such as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To wetting powder, spendable auxiliary agent has: in dispersant such as polycarboxylate, lignosulfonates, the alkylnaphthalene sulfonate one or more; In wetting agent such as alkyl sulfate, alkylsulfonate, the naphthalene sulfonate one or more; Filler such as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To suspending agent, spendable auxiliary agent has: dispersant such as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), TERSPERSE 2020(U.S. Huntsman Corporation produce the alkyl naphthalene sulfonic acid salt) in one or more; Triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), among the TERSPERSE 4894 one or more the polyoxyethylene sorbitan stearate), agricultural newborn 1601#(common name Span60), Tween-60 #(common name alkyl phenol formaldehyde resin polyoxyethylene ether), farming breast 2201, this dish-60#(common name emulsifier such as Nongru-700 #(common name::::; Among wetting agent such as alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, the TERSPERSE 2500 one or more; In thickener such as xanthans, polyvinyl alcohol, bentonite, the aluminium-magnesium silicate one or more; In preservative such as formaldehyde, benzoic acid, the Sodium Benzoate one or more; Defoamer such as organic silicon defoamer; In antifreezing agent such as ethylene glycol, propane diols, glycerine, urea, inorganic salts such as the sodium chloride one or more; Water is deionized water.
Component of the present invention is reasonable; treatment adds protective effect; bactericidal effect is good; reduce the medication number of times, drug cost is low, and its active and bactericidal effect is not the simple superposition of each composition activity; but significant synergistic effect is arranged; slow down the generation of resistance, good to crop safety, meet the security requirement of pesticidal preparations.The present invention has the better prevention effect to grape, strawberry, eliminating vegetable botrytis, alternaria leaf spot of apple, rice sheath blight disease, rice blast.
Embodiment
For grape, strawberry, the eliminating vegetable botrytis of preventing and treating agricultural production, alternaria leaf spot of apple, rice sheath blight disease, rice blast.The inventor has carried out mutual composite synergy research with SYP-3375 and cyprodinil, and concrete grammar is:
Subjects is respectively Botrytis cinerea bacterium Botrytis cinerea Pers, alternaria leaf spot of apple bacterium Alternariaalternataf.sp.mali, Rhizoctonia solani Kuhn (Rhizoctonia solani), the Pyricularia oryzae (Pyriculariagrisea) that causes the tomato gray mould disease pathogen.The former medicine of test medicine SYP-3375 adopts Jilin eight to reach the former medicine that agricultural chemicals Co., Ltd produces, the former medicine that the former medicine of cyprodinil adopts Jiangsu Zhongqi Chemical Industry Co., Ltd to produce.By technology department of Shanggezhilu Biosciences Co., Ltd., Shaanxi former medicine is mixed with the test medicine that needs, test method is with reference to " the agricultural industry criteria NY/T1156.2-2006 of the People's Republic of China (PRC) ".At first single agent and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on preliminary experiment result's basis, different with proportioning according to different medicament combinations, the concentration gradient setting is also different, and bacteriostasis rate is pressed arithmetic series and set in 5%～90% scope).If the clear water contrast repeats 3 times.Adopting the mycelial growth rate method, with cultured pathogen, is 5 mm card punch with diameter under aseptic condition, cuts the bacterium cake from colony edge, with transfer needle the bacterium cake is connected on the culture medium flat plate of different drug concentrations, puts in 25 ℃ of incubators and cultivates 72h; Check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, the EC of each medicament is obtained in the linear regression analysis between the probit value by inhibiting rate and the logarithm value of series concentration
50Value with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, is estimated reagent agent to the activity of germ with this.
The co-toxicity coefficient of complex preparation (CTC) 〉=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.
Toxicity Determination result shows: SYP-3375 and cyprodinil are that 1:30～10:1 uses with graw mold of tomato germ, alternaria leaf spot of apple bacterium, Rhizoctonia solani Kuhn, Pyricularia oryzae are had virulence preferably with the weight ratio, and significant synergistic effect is all arranged.
Below in conjunction with FORMULATION EXAMPLE and biological Examples content of the present invention is described further.
FORMULATION EXAMPLE 1
Take by weighing 10% SYP-3375,40% cyprodinil, 3% TERSPERSE 2700(polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 3% Nekal BX (nekal), 2% K-12(lauryl sodium sulfate), 3% diatomite, 5% glucose, kaolin adds to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is namely mixed, 50% SYP-3375 cyprodinil water dispersible granules is produced in the pulverizing of ultra micro air-flow, mixing, granulation step.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 50%, form new embodiment.
FORMULATION EXAMPLE 2
Take by weighing 2% SYP-3375,60% cyprodinil, 3% TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 1%K-12,1% carboxymethyl cellulose, 3% urea, diatomite and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is namely mixed, 62% SYP-3375 cyprodinil water dispersible granules is produced in the pulverizing of ultra micro air-flow, mixing, granulation step.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 62%, form new embodiment.
FORMULATION EXAMPLE 3
Take by weighing 45% SYP-3375,45% cyprodinil, 3% TERSPERSE 2700,1%K-12,2% sulphur ammonium, diatomite and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is namely mixed, 90% SYP-3375 cyprodinil water dispersible granules is produced in the pulverizing of ultra micro air-flow, mixing, granulation step.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 90%, form new embodiment.
FORMULATION EXAMPLE 4
Take by weighing 40% SYP-3375,4% cyprodinil, 5% Morwet D-425(alkyl naphthalene sulfonic acid condensation polymer sodium salt, Akzo Nobel N.V. produces), 3% dispersing agent NNO, 2% Nekal BX, 2%K-12, kaolin add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is namely mixed, 44% SYP-3375 cyprodinil water dispersible granules is produced in the pulverizing of ultra micro air-flow, mixing, granulation step.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 44%, form new embodiment.
FORMULATION EXAMPLE 5
Take by weighing 20% SYP-3375,60% cyprodinil, 3% TERSPERSE 2700,1% dispersing agent NNO, 2%K-12,1% carboxymethyl cellulose, diatomite and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule is namely mixed, 80% SYP-3375 cyprodinil water dispersible granules is produced in the pulverizing of ultra micro air-flow, mixing, granulation step.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 80%, form new embodiment.
FORMULATION EXAMPLE 6
Take by weighing 20% SYP-3375,30% cyprodinil, 2% dispersing agent NNO, 2% Nekal BX, 2%K-12, attapulgite and add to 100% weight portion.Above-mentioned raw materials is through mixing, and air-flow makes 50% SYP-3375 cyprodinil wetting powder after pulverizing.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 50%, form new embodiment.
FORMULATION EXAMPLE 7
Take by weighing 8% SYP-3375,40% cyprodinil, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% White Carbon black, fine particle calcium carbonate and add to 100% weight portion.Above-mentioned raw materials is through mixing, and air-flow makes 48% SYP-3375 cyprodinil wetting powder after pulverizing.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 48%, form new embodiment.
FORMULATION EXAMPLE 8
Take by weighing 30% SYP-3375,15% cyprodinil, 3% calcium lignosulfonate, 3% dispersing agent NNO, 2% Nekal BX, 2%K-12, White Carbon black and add to 100% weight portion.Above-mentioned raw materials is through mixing, and air-flow makes 45% SYP-3375 cyprodinil wetting powder after pulverizing.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 45%, form new embodiment.
FORMULATION EXAMPLE 9
Take by weighing 15% SYP-3375,35% cyprodinil, 4% calcium lignosulfonate, 3% TERSPERSE 2700,2% Nekal BX, 2%K-12,3% White Carbon black, fine particle calcium carbonate and add to 100% weight portion.Above-mentioned raw materials is through mixing, and air-flow makes 50% SYP-3375 cyprodinil wetting powder after pulverizing.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 50%, form new embodiment.
FORMULATION EXAMPLE 10
Take by weighing 20% SYP-3375,60% cyprodinil, 4% dispersing agent NNO, 2% Nekal BX, 2%K-12, attapulgite and add to 100% weight portion.Above-mentioned raw materials is through mixing, and air-flow makes 80% SYP-3375 cyprodinil wetting powder after pulverizing.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 80%, form new embodiment.
FORMULATION EXAMPLE 11
Taking by weighing 2% SYP-3375,3% cyprodinil, 4% TERSPERSE 4894(U.S. Huntsman Corporation produces), 1.5% TERSPERSE 2500(U.S. Huntsman Corporation produces), 2% TERSPERSE 2020(U.S. Huntsman Corporation produces), 1% aluminium-magnesium silicate, 0.2% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 5% SYP-3375 cyprodinil suspending agent behind the sand mill sand milling.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 5%, form new embodiment.
FORMULATION EXAMPLE 12
Take by weighing 15% SYP-3375,25% cyprodinil, 5%TERSPERSE 4894,2%TERSPERSE 2500,1%TERSPERSE 2020,0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 40% SYP-3375 cyprodinil suspending agent behind the sand mill sand milling.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 40%, form new embodiment.
FORMULATION EXAMPLE 13
Take by weighing 20% SYP-3375,20% cyprodinil, 2%TERSPERSE 2020,2% this dish-60#, 3% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 40% SYP-3375 cyprodinil suspending agent behind the sand mill sand milling.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 40%, form new embodiment.
FORMULATION EXAMPLE 14
Take by weighing 36% SYP-3375,12% cyprodinil, 1%TERSPERSE 2020,3% agricultural newborn 1601#, 2% agricultural newborn 2201#, 1%TERSPERSE 2500,0.3% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.High speed shear is disperseed 30min, with making 48% SYP-3375 cyprodinil suspending agent behind the sand mill sand milling.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 48%, form new embodiment.
FORMULATION EXAMPLE 15
Take by weighing 8% SYP-3375,42% cyprodinil, 4%TERSPERSE 4894,1.5%TERSPERSE 2500,1%TERSPERSE 2020,0.2% aluminium-magnesium silicate, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 50% SYP-3375 cyprodinil suspending agent behind the sand mill sand milling.
The part by weight of SYP-3375 and cyprodinil can change between 1:30～10:1 in the present embodiment, and both total weight items still are 50%, form new embodiment.
Biological Examples 1: control graw mold of tomato field trial
Carried out FORMULATION EXAMPLE 1(50% SYP-3375 cyprodinil water dispersible granules at Shaanxi Province's Yangling District in 2011) control graw mold of tomato field trial, verified that this medicament is to the control efficiency of graw mold of tomato, effective dose and to the safety of tomato.Test method is with reference to " GB/T 17980.28-2000 pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating eliminating vegetable botrytis ".Trial crops is tomato, and controlling object is graw mold of tomato (Botrytis cinereaPers.).Test is located at the Shaanxi Yangling District, and soil is sandy loam, and fertility is good, experimental field the cultivation condition uniformity.
Test medicine and dosage are: 50% SYP-3375 cyprodinil water dispersible granules 35g/667 ㎡, 30g/667 ㎡, 25g/667 ㎡, 20% SYP-3375 suspending agent 25g/667 ㎡, 50% cyprodinil water dispersible granules 80g/667 ㎡.Other establishes blank, and every processing 4 times repeats, every residential quarter area 30 ㎡, and totally 24 residential quarters, district's group is arranged at random.Adopt conventional spray-on process, mu dispenser liquid 60 kg evenly are sprayed at leave dual sides.In dispenser for the first time on May 15, at this moment tomato is in and comes into bloom, and later on every dispenser in 7 days 1 time, dispenser is three times altogether.
Investigation and statistical method:
Carried out the disease index investigation respectively before the dispenser first time and after the dispenser for the first time behind 7 days, dispenser for the third time in 14 days, investigate altogether 3 times, 5 points are investigated in each residential quarter, every some investigation 3 strain tomatoes, record total number of sheets, the sick number of sheets at different levels and sick progression, calculate disease index.
Grade scale is:
1 grade: blade does not have scab
2 grades: fragmentary little scab appears in blade
3 grades: lesion area accounts for the 1/6-l/4 of leaf area
4 grades: lesion area accounts for leaf area 1/4-l/2
5 grades: lesion area accounts for leaf area more than 1/2 or causes fallen leaves
Disease index and control efficiency computational methods:
Disease index=Σ (the sick numbers of sheets at different levels * relative level numerical value)/investigate total number of sheets * 5 * 100%
Disease index * 100% after control efficiency (%)=(disease index after disease index after the dispenser of blank district-chemicals treatment district dispenser)/blank district dispenser
Security survey and to the influence of other biological: sprayed behind the medicine the 1st day at every turn and medicine after some days, each handles influence to tomato to observe medicament.
Field test results shows: 50% SYP-3375 cyprodinil water dispersible granules 35g/667 ㎡, 30g/667 ㎡, 25g/667 ㎡ prevent and treat graw mold of tomato, 7 days preventive effect is respectively 81.25%, 77.28%, 72.59% behind the medicine for the first time, 14 days preventive effect is respectively 92.22%, 88.41%, 85.36% behind the medicine for the third time, obviously be better than 20% SYP-3375 suspending agent 25g/667 ㎡, the graw mold of tomato of 50% cyprodinil water dispersible granules 80g/667 ㎡ is 7 days and 14 days preventive effect (seeing Table 5) behind the medicine for the third time behind the medicine for the first time, shows good quick-acting and lasting effect.
Duration of test is observed, and each chemicals treatment does not produce the poisoning phenomenon to tomato, and 50% SYP-3375 cyprodinil water dispersible granules also shows good control efficiency to tomato blight, leaf mold.
Result of the test has obviously improved the control efficiency to graw mold of tomato after showing that SYP-3375 and cyprodinil are mixed, and has reduced dosage and drug cost, to tomato growth safety, is the desirable medicament of control graw mold of tomato.
Biological Examples 2: control alternaria leaf spot of apple field trial
Carried out FORMULATION EXAMPLE 7(48% SYP-3375 cyprodinil wetting powder in 2010 in the Fuping County, Shaanxi Province) control alternaria leaf spot of apple field trial, verified that this medicament is to the control efficiency of alternaria leaf spot of apple, effective dose and to the safety of apple.Test method is with reference to " pesticide field efficacy medicine test criterion (two) GB/T 17980.124-2004 the 124th part: the bactericidal agent for preventing and treating alternaria leaf spot of apple test of pesticide effectiveness ".Trial crops is apple tree, and controlling object is alternaria leaf spot of apple (Alternaria alternaria f.sp mali).Test being located at Fuping County, Shaanxi Province stream Qu Zhen, the orchard soil middle fertility, and fruit tree is to plant in 2000, and kind be the Fuji apple, and test once prevented and treated aphid with 3% acetamiprid EC preceding January, experimental field the cultivation condition uniformity.Test medicine and dosage are: 1500 times, 2000 times, 2500 times of 48% SYP-3375 cyprodinil wetting powders, 2000 times of 20% SYP-3375 suspending agents, 800 times of 50% cyprodinil water dispersible granules.Other establishes blank, and every processing 4 times repeats, 3 trees in every residential quarter, and totally 24 residential quarters, district's group is arranged at random.Adopt conventional spray-on process, the 1st dispenser when the blade beginning is seen scab, every dispenser in 10-15 days 1 time, dispenser is 3 times altogether with backsight disease development.Evenly spray each position of tree body, drenched degree of being with limb, fruit and leave dual sides by soup.
Investigation and statistical method, 7 days investigation drug effects behind medicine for the third time, 2 strains tree is investigated in every residential quarter, east, south, west, north, in 6 orientation get 6 points, 2 branch of every some investigation are investigated whole blades, record total number of sheets, the sick number of sheets, sick leaf rate, according to 9 grades of grade scales of alternaria leaf spot of apple, put down in writing the sick numbers of sheets at different levels, calculate disease index and preventive effect.Behind each spray medicine behind the 1st day and the medicine some days, each handled influence to fruit and blade to observe medicament.
The alternaria leaf spot of apple grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 10% of whole leaf area;
3 grades: lesion area accounts for the 11%-25% of whole leaf area;
5 grades: lesion area accounts for the 26%-40% of whole leaf area;
7 grades: lesion area accounts for the 41%-65% of whole leaf area;
9 grades: lesion area accounts for more than 66% of whole leaf area.
Calculate disease index and control efficiency according to the record incidence.
Disease index=Σ (the sick numbers of sheets at different levels * relative level numerical value)/investigate total number of sheets * 9 * 100%
Disease index * 100% after control efficiency (%)=(disease index after disease index after the dispenser of blank district-chemicals treatment district dispenser)/blank district dispenser.
By each residential quarter preventive effect being carried out biostatistics analysis (seeing Table 6), significance of difference comparative result shows: consistent with the significance of difference on 0.01 level on 0.05 level, significant difference between 1500 times, 2000 times of the 48% SYP-3375 cyprodinil wetting powders is all respectively handled differences with other and is all reached utmost point significance level; 2500 times of 48% SYP-3375 cyprodinil wetting powders are not remarkable with 2000 times of differences of 20% SYP-3375 suspending agent, with 800 times of significant differences of 50% cyprodinil water dispersible granules; Difference is not remarkable between 800 times of 2000 times of 20% SYP-3375 suspending agents and the 50% cyprodinil water dispersible granules.
Field test results shows: 48% SYP-3375 cyprodinil wetting powder has than remarkable prevention effect alternaria leaf spot of apple, and its 1500 times, 2000 times, the 2500 times control efficiency to alternaria leaf spot of apple obviously are better than the control efficiency of 2000 times of 20% SYP-3375 suspending agents, 800 times of 50% cyprodinil water dispersible granules.
To the security survey of apple tree, to observe in some days behind the 1st day and the medicine behind the spray medicine, each test is handled not to be had the poisoning phenomenon to fruit, blade and takes place.
Result of the test has obviously improved the control efficiency to alternaria leaf spot of apple after showing that SYP-3375 and cyprodinil are mixed, and has reduced dosage and drug cost, to apple development safety, is the desirable medicament of control alternaria leaf spot of apple.
Biological Examples 3: control rice blast field trial
Carried out FORMULATION EXAMPLE 12(40% SYP-3375 cyprodinil suspending agent in 2010 in Xiuqian City, Jiangsu Province) control rice blast field trial, verify that this medicament is to the control efficiency of rice blast, effective dose and to the safety of paddy rice.Trial crops is paddy rice, and controlling object is rice blast (Pyricularia oryzae Cav.).Test is located at Siyang County, Xiuqian City, and test medicine and dosage are: 40% SYP-3375 cyprodinil suspending agent 30g/667 ㎡, 25g/667 ㎡, 20g/667 ㎡, 20% SYP-3375 suspending agent 25g/667 ㎡, 50% cyprodinil water dispersible granules 80g/667 ㎡.Other establishes blank, and every processing 4 times repeats, every residential quarter area 35 ㎡, and totally 24 residential quarters, district's group is arranged at random.Adopt conventional spray-on process, mu dispenser liquid 60kg evenly sprays the paddy rice complete stool.Altogether dispenser is 2 times, paddy rice booting latter stage and each dispenser of full heading time 1 time.
Investigation and statistical method:
Investigation in 14 days after the 2nd dispenser, investigation method is 5 samplings in every residential quarter, every some investigation 50 fringes, grade scale is as follows:
0 grade: anosis;
1 grade: every fringe loss is (morbidity of indivedual branch stalk) below 5%;
3 grades: every fringe loss 6%-20% (branch stalk morbidity about 1/3);
5 grades: every fringe loss 21%-50% (fringe neck or main shaft morbidity, grain half is flat);
7 grades: every fringe loss 51%-70% (morbidity of fringe neck, most of shrivelled kernel);
9 grades: every fringe loss 71%-100% (morbidity of fringe neck causes dead ears).
Refer to calculate preventive effect according to disease.Behind each spray medicine behind the 1st day and the medicine some days, each handled influence to paddy rice to observe medicament.
Disease index=Σ (sick spike numbers at different levels * relative level numerical value)/investigate total spike number * 9 * 100%
Disease index * 100% after control efficiency (%)=(disease index after disease index after the dispenser of blank district-chemicals treatment district dispenser)/blank district dispenser.
By each residential quarter preventive effect being carried out biostatistics analysis (seeing Table 7), significance of difference comparative result shows: consistent with the significance of difference on 0.01 level on 0.05 level, significant difference between 40% SYP-3375 cyprodinil suspending agent 30g/667 ㎡, three various dose of 25g/667 ㎡, 20g/667 ㎡, all with SYP-3375 suspending agent 25g/667 ㎡, 50% cyprodinil water dispersible granules 80g/667 ㎡ difference is extremely remarkable; Difference is not remarkable between SYP-3375 suspending agent 25g/667 ㎡ and the 50% cyprodinil water dispersible granules 80g/667 ㎡.
Field test results shows: 40% SYP-3375 cyprodinil suspending agent has than remarkable prevention effect rice blast, and it obviously is better than the control efficiency of SYP-3375 suspending agent 25g/667 ㎡, 50% cyprodinil water dispersible granules 80g/667 ㎡ to the control efficiency of rice blast by 30g/667 ㎡, 25g/667 ㎡, 20g/667 ㎡ consumption.
To the security survey of paddy rice, to observe in some days behind the 1st day and the medicine behind the spray medicine, each test is handled not to be had the poisoning phenomenon to paddy rice and takes place.
Result of the test has obviously improved the control efficiency to rice blast after showing that SYP-3375 and cyprodinil are mixed, and has reduced dosage and drug cost, to paddy growth safety, is the desirable medicament of control rice blast.
In sum, composition of the present invention is to adopt two kinds of active component compound schemes, its activity and bactericidal effect are not the simple superposition of each composition activity, compare with existing unitary agent, except having significant bactericidal effect, and significant synergistic effect arranged, slow down the generation of resistance, the preparation less residue, pollution-free to crop, safety is good, meets the security requirement of pesticidal preparations.
Claims (7)
1. a bactericidal composition that contains SYP-3375 and cyprodinil is characterized in that the composition active component is SYP-3375 and cyprodinil, and the weight ratio of SYP-3375 and cyprodinil is 1:30～10:1.
2. according to the described bactericidal composition that contains SYP-3375 and cyprodinil of claim 1, it is characterized in that: the weight ratio of SYP-3375 and cyprodinil is 1:10～4:1.
3. according to the described bactericidal composition that contains SYP-3375 and cyprodinil of claim 2, it is characterized in that: the weight ratio of SYP-3375 and cyprodinil is 1:5～2:1.
4. according to one of any described bactericidal composition that contains SYP-3375 and cyprodinil of claim 1 to 3, it is characterized in that: the cumulant of SYP-3375 and cyprodinil is 1%～90% of described composition total weight.
5. according to the described bactericidal composition that contains SYP-3375 and cyprodinil of claim 4, it is characterized in that: the cumulant of SYP-3375 and cyprodinil is 5%～50% of described composition total weight.
6. according to the described bactericidal composition that contains SYP-3375 and cyprodinil of claim 1, it is characterized in that: described composition is water dispersible granules, wetting powder, suspending agent.
7. according to the described purposes of bactericidal composition on control grape grey mould, grey mould fruit rot of strawberry, eliminating vegetable botrytis, alternaria leaf spot of apple, rice sheath blight disease, rice blast that contains SYP-3375 and cyprodinil of claim 1.
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| CN102939969A (en) * | 2012-09-21 | 2013-02-27 | 吉林省八达农药有限公司 | Agricultural fungicidal composition containing coumoxystrobin and ethirimol |
| CN103004772A (en) * | 2012-11-01 | 2013-04-03 | 海利尔药业集团股份有限公司 | Bactericidal composition containing pyraoxystrobin and cyprodinil |
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Effective date of registration: 20170122 Address after: 136100 Gongzhuling national agricultural science and Technology Park in Jilin province Nan Wei Zi Zhen Jie Dong Patentee after: Jilin Bada Pesticide Co., Ltd. Address before: 9 No. 710404 Shaanxi province Xi''an Zhouzhi County of Jixian City Industrial Park Venture Road Patentee before: Shanggezhilu Biosciences Co., Ltd., Shaanxi |