CN102388888B - Sterilization composite containing coumoxystrobin and fludioxonil - Google Patents
Sterilization composite containing coumoxystrobin and fludioxonil Download PDFInfo
- Publication number
- CN102388888B CN102388888B CN201110304798.5A CN201110304798A CN102388888B CN 102388888 B CN102388888 B CN 102388888B CN 201110304798 A CN201110304798 A CN 201110304798A CN 102388888 B CN102388888 B CN 102388888B
- Authority
- CN
- China
- Prior art keywords
- fludioxonil
- syp
- sterilization
- agent
- tersperse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a sterilization composite containing coumoxystrobin and fludioxonil, wherein the weight ratio of coumoxystrobin to fludioxonil is 1:20-3:1. In the invention, wettable powder, suspension agent, water dispersible granule and suspension seed coating formulation accepted by the agriculture are prepared. In the sterilization composite, the component contents are reasonable, the sterilization effect is good, the dosing cost is low, the activity and the sterilization effect are not the superposition of the activities and the sterilization effects of the components. Compared with the single preparation, the sterilization composite containing coumoxystrobin and fludioxonil has obvious synergism besides the obvious sterilization effect, slows down the generation of resistance, is small in the residual amount of the preparation, is free of pollution on crops and good in safety, conforms to the requirement on safety of the pesticide preparation, and has obvious preventive effects on vegetables, fruits, strawberry anthracnose, botrytis and rice bakanae.
Description
Technical field
The present invention relates to a kind of bactericidal composition, particularly a kind of bactericidal composition containing SYP-3375 and fludioxonil, belongs to technical field of compounded pesticide.
Background technology
Methoxy acrylic bactericide is in recent years bactericide kind newly developed, is a large milestone in bactericide field.Methoxy acrylic bactericide has unique mechanism of action, and it stops the ATP of cell synthetic by the electronics transmission of pinning between cytochrome b and c1, thereby suppresses its mitochondrial respiratory and bring into play bacteriostasis.The bacterial strain that 14-demethylation enzyme inhibitor, benzamides, dicarboxylic dihydrazides amine and benzimidazole is produced to resistance is effective.There is protection, treat, root out, infiltration, systemic activity, and can be in plant corpus, degraded very soon in soil and water.Although this series bactericidal agent mechanism of action is unique, but the speed that pathogen produces resistance to it is also very fast, use cost is high, so having limited the spread of methoxy acrylic bactericide uses, therefore the bactericide combination of methoxy acrylic bactericide and the another kind of mechanism of action is composite, improve drug effect, slow down the generation of resistance, reduce usage amount, expanding using of this medicament is current urgent problem.
SYP-3375 is a kind of methoxy-methyl acrylate compounds, English common name: coumoxystrobin, chemical name: (E)-2-(2-((3-butyl-4-methyl-coumarin-7-Ji-oxygen base) methyl) phenyl)-3-methoxy-methyl acrylate.There is protection and therapeutic action; a kind of disinfectant use in agriculture of high-efficiency broad spectrum, to having good preventive effect by mastigomycetes, the plant disease that causes in conjunction with bacterium, sac fungi, basidiomycetes and imperfect fungus as sclerotinia rot of colza, apple valsa canker, cucumber downy mildew, powdery mildew of cucumber, rice blast, sigatoka, alternaria leaf spot of apple etc.
Fludioxonil, English common name: fludioxonil, other titles: suitable when happy, chemical name: 4-2,2-bis-is fluoro-1,3-benzo dioxy-4-yl) pyrroles-3-nitrile.Fludioxonil is broad-spectrum touch-out bactericide, for seed treatment, can prevent and treat that major part is carried disease germs and the fungal disease of soil infection.Stable in soil, at seed and seedling rhizosphere, form protection zone, prevent germ invasion.As blade face bactericide, being used for preventing and treating the rotten Fusariumsp of snow, wheat net raw meat black rot. rhizoctonia solanis etc., have special efficacy to gray mold; Novel structure, is difficult for and other bactericide generation cross resistance.
Although SYP-3375 is a kind of disinfectant use in agriculture of high-efficiency broad spectrum, it is as methoxy acrylic ester compounds, and the mechanism of action is single, reuses for a long time germ and can produce resistance in the application of field, cause preventive effect to reduce, and use cost is high.Fludioxonil has been used for many years, and resistance increases year by year, uses separately dosage increasing.The technology report that does not also have at present SYP-3375 and fludioxonil to be mixed.Inventor finds by test, and SYP-3375 and fludioxonil that the mechanism of action is different is composite, has obvious synergistic effect; can significantly improve control efficiency, minimizing spraying times and the dosage for the treatment of, protection; slow down the generation of germ resistance, reduce cost accounting, high to crop safety.
Summary of the invention
The object of the present invention is to provide a kind of component reasonable, synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, is difficult for developing immunity to drugs, the agricultural bactericidal composition to crop safety.
Another object of the present invention is to provide the application of above-mentioned composition in control vegetables, fruit tree, Strawberry anthracnose, gray mold, bakanae disease of rice.
In order to overcome the defect of existing unitary agent, technical scheme of the present invention is to solve like this:
A) the first active component SYP-3375;
B) the second active component fludioxonil;
The weight ratio of the first active component and the second active component is 1:20～3:1, and preferred weight ratio is 1:15～2:1, and preferred weight ratio is 1:10～1:1.
The cumulant of the first active component and the second active component is 1%～80% of described composition total weight, is preferably 5%～70%, more preferably 10%～50%.The size of active component cumulant is also closely related with formulation, as the active component cumulant of suspension seed-coating agent, is no more than 50%.
The preparation formulation that the agricultural bactericidal composition that the present invention contains SYP-3375 and fludioxonil can be prepared according to the known method of those skilled in the art of the present technique is wetting powder, suspending agent, water dispersible granules, suspension seed-coating agent.
To wetting powder, spendable auxiliary agent has: dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Filler as ammonium sulfate, urea, sucrose, glucose, diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To suspending agent, spendable auxiliary agent has: dispersant is produced as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate (dispersing agent NNO), TERSPERSE 2020(U.S. Huntsman Corporation, alkyl naphthalene sulfonic acid salt) in one or more; Polyoxyethylene sorbitan stearate), agriculture breast 1601#(common name Span60), Tween-60 #(common name alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this dish-60#(common name emulsifier is as Nongru-700 #(common name:::: one or more triphen ethyl-phenol polyoxyethylene polyoxypropylene block polymer), in TERSPERSE 4894; Wetting agent as in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 one or more; Thickener as in xanthans, polyvinyl alcohol, bentonite, aluminium-magnesium silicate one or more; Preservative as in formaldehyde, benzoic acid, Sodium Benzoate one or more; Defoamer is as organic silicon defoamer; Antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more; Water is deionized water.
For water dispersible granules, those skilled in the art are familiar with the corresponding auxiliary agent of use and complete the present invention very much.Dispersant as in polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate one or more; Wetting agent as in alkyl sulfate, alkylsulfonate, naphthalene sulfonate one or more; Disintegrant as in ammonium sulfate, urea, sucrose, glucose one or more; Binding agent is as one or more in diatomite, corn starch, PVA, carboxymethyl (ethyl) cellulose family; Filler as diatomite, kaolin, white carbon, fine particle calcium carbonate, talcum powder, attapulgite, potter's clay one or more.
To suspension seed-coating agent, spendable auxiliary agent has: binding agent is as polysaccharose macromolecular compound (soluble starch, polypropylene graft copolymer, xanthan gum, microorganism mucilage), cellulose derivatives (hydroxypropyl methylcellulose, sodium carboxymethylcellulose, ethyl cellulose), seaweeds is as sodium alginate, agar, rosin, paraffin, gelatin, pectin, polyvinyl alcohol, polyethylene glycol, polyvinyl acetate, polyacrylamide, the water-soluble composite of polyvinylpyrrolidone and polyhydric alcohol polymer, inorganic binder (aluminium-magnesium silicate, clay, waterglass, gypsum) in one or more, dispersant is produced as polycarboxylate, lignosulfonates, alkylnaphthalene sulfonate, TERSPERSE 2020(U.S. Huntsman Corporation, alkyl naphthalene sulfonic acid salt) in one or more, sorbitan monostearate APEO), agriculture breast 1601#(common name sorbitan monostearate), emulsifier T-60(common name alkyl phenol formaldehyde resin polyoxyethylene ether), agriculture breast 2201, this dish-60#(common name emulsifier is as Nongru-700 #(common name:::: one or more phenethyl phenol polyoxyethylene poly-oxygen propylene aether), in TERSPERSE 4894, wetting agent as in alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate, naphthalene sulfonate, TERSPERSE 2500 one or more, thickener as in xanthans, polyvinyl alcohol, bentonite one or more, preservative as in formaldehyde, benzoic acid, Sodium Benzoate one or more, defoamer is as organic silicon defoamer, antifreezing agent as ethylene glycol, propane diols, glycerine, urea, inorganic salts as in sodium chloride one or more, water is deionized water.
Component of the present invention is reasonable; treatment adds protective effect; bactericidal effect is good; reduce medication number of times, drug cost is low, and its active and bactericidal effect is not the simple superposition of each composition activity; but there is significant synergistic effect; slow down the generation of resistance, good to crop safety, meet the security requirement of pesticidal preparations.The present invention has good control efficiency to vegetables, fruit tree, Strawberry anthracnose, gray mold, bakanae disease of rice.
Embodiment
In order to prevent and treat vegetables, fruit tree, Strawberry anthracnose, gray mold, the bakanae disease of rice in agricultural production, inventor is to representational Chinese cabbage anthracnose, bakanae disease of rice and graw mold of tomato, with SYP-3375 and fludioxonil, carried out mutual composite synergy research, concrete grammar is:
Subjects be respectively cause the pathogenetic John Higgins thorn of Chinese cabbage anthrax dish spore bacterium (Col-letotrichum higginsianum Sacc.), cause the pathogenetic the pathogen of Botrytis cinerea of tomato gray mould (Botrytis cinerea Pers), fusarium moniliforme (Fusarium moniliforme Sheld.).The former medicine that the former medicine of test medicine SYP-3375 adopts Jilin Ba Da agricultural chemicals Co., Ltd to produce, the former medicine that the former medicine of fludioxonil adopts Switzerland Xian Zhengda crop protection Co., Ltd to produce.By technology department of Shanggezhilu Biosciences Co., Ltd., Shaanxi, former medicine is mixed with to the test medicine needing, test method is with reference to < < People's Republic of China's agricultural industry criteria NY/T1156.2-2006 > >.First single dose and each Mixed Pharmacy are arranged to 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different medicament combinations, concentration gradient setting is also different, and bacteriostasis rate is pressed arithmetic series and set in 5%～90% scope).If clear water contrast, repeats 3 times.Adopt mycelial growth rate method, by cultured pathogen, under aseptic condition, with diameter, be 5 mm card punch, from colony edge, cut bacterium cake, with transfer needle, bacterium cake is connected on the culture medium flat plate of different drug concentrations, put in 26 ℃ of incubators and cultivate, Chinese cabbage anthrax bacteria, botrytis cinerea are cultivated 72 hours, and bakanae disease of rice is cultivated 7 days; Check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC of each medicament
50value, with the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun mixture, evaluates the activity of reagent agent to germ with this.
The co-toxicity coefficient of complex preparation (CTC) >=120 shows as synergistic effect; CTC≤80 show as antagonism; 80 < CTC < 120 show as summation action.
Toxicity Determination result shows: SYP-3375 be take weight ratio has good virulence as 1:20～3:1 is mixed to Chinese cabbage anthrax bacteria, botrytis cinerea, fusarium moniliforme with fludioxonil, all has significant synergistic effect.
Below in conjunction with FORMULATION EXAMPLE and biological Examples, content of the present invention is described further.
FORMULATION EXAMPLE 1
Take 20% SYP-3375,60% fludioxonil, 3% TERSPERSE 2700(polycarboxylate, U.S. Huntsman Corporation produces), 2% dispersing agent NNO (alkylnaphthalene sulfonate formaldehyde condensation products), 2% K-12(lauryl sodium sulfate), attapulgite adds to 100% weight portion.Above-mentioned raw materials, through mixing, makes 80% SYP-3375 fludioxonil wetting powder after air-flow crushing.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 80%, form new embodiment.
FORMULATION EXAMPLE 2
Take 3% SYP-3375,60% fludioxonil, 4% calcium lignosulfonate, 3% TERSPERSE 2700,1% Nekal BX (nekal), 1%K-12,3% White Carbon black, fine particle calcium carbonate and add to 100% weight portion.Above-mentioned raw materials, through mixing, makes 63% SYP-3375 fludioxonil wetting powder after air-flow crushing.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 63%, form new embodiment.
FORMULATION EXAMPLE 3
Take 5% SYP-3375,25% fludioxonil, 4% calcium lignosulfonate, 4% dispersing agent NNO, 2% Nekal BX, 2%K-12, White Carbon black and add to 100% weight portion.Above-mentioned raw materials, through mixing, makes 30% SYP-3375 fludioxonil wetting powder after air-flow crushing.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 30%, form new embodiment.
FORMULATION EXAMPLE 4
Take 20% SYP-3375,30% fludioxonil, 5% calcium lignosulfonate, 3% TERSPERSE 2020(alkyl naphthalene sulfonic acid salt, U.S. Huntsman Corporation produces), 2% Nekal BX, 2%K-12,5% White Carbon black, kaolin add to 100% weight portion.Above-mentioned raw materials, through mixing, makes 50% SYP-3375 fludioxonil wetting powder after air-flow crushing.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 50%, form new embodiment.
FORMULATION EXAMPLE 5
Take 4% SYP-3375,32% fludioxonil, 5% TERSPERSE 4894(U.S. Huntsman Corporation produces), 2% TERSPERSE 2500(U.S. Huntsman Corporation produces), 1% TERSPERSE 2020(U.S. Huntsman Corporation produces), 0.2% xanthans, 5% propane diols, 0.5% benzoic acid, 0.5% silicone defoaming agent, deionized water add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 36% SYP-3375 fludioxonil suspending agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 36%, form new embodiment.
FORMULATION EXAMPLE 6
Take 20% SYP-3375,10% fludioxonil, 1%TERSPERSE 2020,3% this dish-60#, 4% Tween-60 #, 1%TERSPERSE 2500,0.5% white carbon, 0.3% xanthans, 5% ethylene glycol, 0.5% benzoic acid, 0.5% silicone defoaming agent (trade name: s-29 Nanjing "four news" (new ideas applied chemistry product company produces), deionized water adds to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 30% SYP-3375 fludioxonil suspending agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 30%, form new embodiment.
FORMULATION EXAMPLE 7
Take 15% SYP-3375,30% fludioxonil, 1%TERSPERSE 2020,3% agriculture breast 1601#, 3% agriculture breast 2201#, 1%TERSPERSE 2500,0.3% xanthans, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.High speed shear is disperseed 30min, with making 45% SYP-3375 fludioxonil suspending agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 45%, form new embodiment.
FORMULATION EXAMPLE 8
Take 5% SYP-3375,20% fludioxonil, 4%TERSPERSE 4894,3% Nongru-700 #, 1.5%TERSPERSE 2500,1%TERSPERSE 2020,0.5% aluminium-magnesium silicate, 5% ethylene glycol, 0.5% formaldehyde, 0.5% silicone defoaming agent, deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 25% SYP-3375 fludioxonil suspending agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 25%, form new embodiment.
FORMULATION EXAMPLE 9
Take 60% SYP-3375,20% fludioxonil, 3% TERSPERSE 2700,3%TERSPERSE 2020(U.S. Huntsman Corporation and produce, alkylnaphthalene sulfonate formaldehyde condensation products), 2%K-12,3% ammonium sulfate, diatomite add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 80% SYP-3375 fludioxonil water dispersible granules.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 80%, form new embodiment.
FORMULATION EXAMPLE 10
Take 20% SYP-3375,50% fludioxonil, 3% TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 2%K-12,3% diatomite, 5% glucose, kaolin and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 70% SYP-3375 fludioxonil water dispersible granules.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 70%, form new embodiment.
FORMULATION EXAMPLE 11
Take 15% SYP-3375,45% fludioxonil, 3% TERSPERSE 2700,3% dispersing agent NNO, 2% Nekal BX, 1%K-12,1% carboxymethyl cellulose, 3% urea, kaolin and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 60% SYP-3375 fludioxonil water dispersible granules.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 60%, form new embodiment.
FORMULATION EXAMPLE 12
Take 5% SYP-3375,60% fludioxonil, 3% TERSPERSE 2700,2%K-12,5% ammonium sulfate, diatomite and add to 100% weight portion.The method of the frequent regulation of above-mentioned raw materials water intaking dispersible granule mixes, ultra micro air-flow crushing, mixing, granulation step are produced 65% SYP-3375 fludioxonil water dispersible granules.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 65%, form new embodiment.
FORMULATION EXAMPLE 13
Take 8% SYP-3375,32% fludioxonil, 1%TERSPERSE 2700,1%TERSPERSE 2500,3% TERSPERSE 4894,2.0% polyvinylpyrrolidone, 0.2% xanthans, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoaming agent deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 40% SYP-3375 fludioxonil suspension seed-coating agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 40%, form new embodiment.
FORMULATION EXAMPLE 14
Take 3% SYP-3375,2% fludioxonil, 3%TERSPERSE 4894,2%TERSPERSE 2500,1%TERSPERSE 2020,2% hydroxypropyl methylcellulose, 0.5% bentonite, 5% ethylene glycol, 0.3% Sodium Benzoate, 0.5% silicone defoaming agent deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 5% SYP-3375 fludioxonil suspension seed-coating agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 5%, form new embodiment.
FORMULATION EXAMPLE 15
Take 15% SYP-3375,6% fludioxonil, 4%TERSPERSE 4894,2% alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, 1% dispersing agent NNO, 2.0% polyvinyl acetate, 0.2% xanthans, 5% propane diols, 0.3% benzoic acid, 0.5% silicone defoaming agent deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 21% SYP-3375 fludioxonil suspension seed-coating agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 21%, form new embodiment.
FORMULATION EXAMPLE 16
Take 5% SYP-3375,5% fludioxonil, 3%TERSPERSE 4894,1%TERSPERSE 2500,0.5%TERSPERSE 2020,2% polyvinyl alcohol (molecular weight is 8000), 1% polyacrylamide, 0.2% xanthans, 5% glycerine, 0.3% benzoic acid, 0.5% silicone defoaming agent deionized water and add to 100% weight portion.Above-mentioned raw materials is through mixing, and high speed shear is disperseed 30min, with making 10% SYP-3375 fludioxonil suspension seed-coating agent after sand mill sand milling.
In the present embodiment, the part by weight of SYP-3375 and fludioxonil can change between 1:20～3:1, and both total weight items are still 10%, form new embodiment.
Biological Examples 1: biocontrol of mango anthracnose field trial
2010 Lingshui county, Nian Hainan Province have carried out FORMULATION EXAMPLE 7(45% SYP-3375 fludioxonil suspending agent) biocontrol of mango anthracnose field trial, verified that this medicament is to the control efficiency of mango anthracnose, effective dose and the safety to mango.Test method is with reference to < < GB/T 17980.98-2004 pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating mango anthracnose test of pesticide effectiveness > >.Trial crops is mango, and controlling object is mango anthracnose (Colletotrichum gloeosporioides).Test is located at Ying Zhou town, Lingshui county Mango Fruit Garden, and kind is ivory awns, the age of tree 12 years, and growing way is general.Be experimental field ground, gentle slope, soil is alluviation sandy soil, and fertility is general, experimental field cultivation condition uniformity.
Test medicine and dosage are: 45% 2500 times, 3000 times, 3500 times of SYP-3375 fludioxonil suspending agents, 20% 1500 times of SYP-3375 suspending agents, 50% 2000 times of fludioxonil wetting powders.Separately establish blank, every processing repeats for 4 times, 5 of every communities tree, and Gong24Ge community, random district group is arranged.By agriculture HD-400 type sprayer even spraying.Mango young fruit period and each dispenser of fruit expanding period 1 time, dispenser is 2 times altogether.
Investigation and statistical method:
Within 10 days after dispenser for the second time, carry out disease index investigation, each community random searching 3 strain, East, West, South, North, middle sampling are pressed in every strain, investigate 10 of fruits at every, and the total fruit of record investigation number, disease fruit number at different levels, calculate disease index.
Grade scale is:
0 grade: anosis;
1 grade: on every young fruit, needle-like brown spot is less than 5;
3 grades: on every young fruit, needle-like brown spot is 6～15;
5 grades: on every young fruit, needle-like brown spot is 16～25;
7 grades: on every young fruit, needle-like brown spot is more than 25, and spot is connected;
9 grades: whole fruit is met personally scab, there is red point-like thing to occur.
Disease index and control efficiency computational methods:
Diseased fruit rate (%)=(the total fruit of investigation number/disease fruit number) * 100
Disease index=[∑ (sick fruit number * relative level numerical value at different levels)/(the total fruit of investigation number * 9)] * 100
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100
Security survey and the impact on other biological: spray after medicine the 1st day at every turn and medicine after some days, each processes the impact on mango to observe medicament.
Field test results shows, 45% SYP-3375 fludioxonil suspending agent has good control efficiency to mango anthracnose, dilute 2500 times, 3000 times, 3500 times sprayings, 10 days aobvious reductions of diseased fruit rate comparison illumination after dispenser for the second time, preventive effect is respectively 87.26%, 83.14%, 77.36%, is obviously better than the preventive effect of 1500 times of 20% SYP-3375 suspending agents, 2000 times of 50% fludioxonil wetting powders.
By medicine Hou10Tian Ge community preventive effect is for the second time carried out to biostatistics Epidemiological Analysis (in Table 4), significance of difference comparative result shows: consistent with the significance of difference in 0.01 level in 0.05 level, significant difference between 45% 2500 times, 3000 times, 3500 times of SYP-3375 fludioxonil suspending agents, all extremely remarkable with 1500 times of 20% SYP-3375 suspending agents, 2000 times of differences of 50% fludioxonil wetting powder, between 2000 times of 20% 1500 times of SYP-3375 suspending agents and 50% fludioxonil wetting powders, difference is not remarkable.
Duration of test is observed, each chemicals treatment not to the flower of mango, really, leaf produces poisoning phenomenon.
Result of the test has obviously improved the control efficiency to mango anthracnose after showing that SYP-3375 and fludioxonil are mixed, and has reduced dosage and drug cost, to Growth of Mango safety, is the desirable medicament of biocontrol of mango anthracnose.
Biological Examples 2: control graw mold of tomato field trial
Within 2011, at Guangxi province Bose City, carried out FORMULATION EXAMPLE 11(60% SYP-3375 fludioxonil water dispersible granules) control graw mold of tomato field trial, verified that this medicament is to the control efficiency of graw mold of tomato, effective dose and the safety to tomato.Test method is with reference to < < GB/T 17980.28-2000 pesticide field efficacy medicine test criterion () bactericidal agent for preventing and treating eliminating vegetable botrytis > >.Trial crops is tomato, and controlling object is graw mold of tomato (Botrytis cinereaPers.).Test is located at Tian Zhou town, Bose City Tianyang County, and soil is sandy loam, and fertility is good, experimental field cultivation condition uniformity.
Test medicine and dosage are: 60% SYP-3375 fludioxonil water dispersible granules 21g/667 ㎡, 18g/667 ㎡, 15g/667 ㎡, 20% SYP-3375 suspending agent 20g/667 ㎡, 50% fludioxonil wetting powder 50g/667 ㎡.Separately establish blank, every processing repeats for 4 times, every community area 30 ㎡, and Gong24Ge community, random district group is arranged.Adopt conventional spray-on process, mu dispenser liquid 60 kg, are evenly sprayed at leave dual sides.In the tomato dispenser for the first time that comes into bloom, later every dispenser in 7 days 1 time, dispenser is three times altogether.
Investigation and statistical method:
Before dispenser for the first time and after dispenser for the first time, behind 7 days, dispenser for the third time, within 14 days, carry out disease index investigation respectively, investigate altogether 3 times, 5 points are investigated in each community, every some investigation 3 strain tomatoes, record total number of sheets, the sick number of sheets at different levels and sick progression, calculate disease index.
Grade scale is:
1 grade: blade is without scab
2 grades: fragmentary little scab appears in blade
3 grades: lesion area accounts for the 1/6-l/4 of leaf area
4 grades: lesion area accounts for leaf area 1/4-l/2
5 grades: lesion area accounts for leaf area more than 1/2 or causes fallen leaves
Disease index and control efficiency computational methods:
Disease index=[∑ (the sick number of sheets * relative level numerical value at different levels)/(investigating the total number of sheets * 5)] * 100
Control efficiency (%)=[(contrast disease index-processing disease index)/contrast disease index] * 100
Security survey and the impact on other biological: spray after medicine the 1st day at every turn and medicine after some days, each processes the impact on tomato to observe medicament.
Duration of test is observed, and each chemicals treatment does not produce poisoning phenomenon to tomato, and 60% SYP-3375 fludioxonil water dispersible granules also shows good control efficiency to tomato blight, leaf mold.
Result of the test has obviously improved the control efficiency to graw mold of tomato after showing that SYP-3375 and fludioxonil are mixed, and has reduced dosage and drug cost, to tomato growth safety, is the desirable medicament of control graw mold of tomato.
Biological Examples 3: prevent and treat bakanae disease of rice field trial
2010 Mudanjiang Cities, Nian Heilongjiang Province have carried out FORMULATION EXAMPLE 16(10% SYP-3375 fludioxonil suspension seed-coating agent) prevent and treat bakanae disease of rice field trial, verified that this medicament is to the control efficiency of bakanae disease of rice, effective dose and the safety to paddy rice.Test method is with reference to < < GB/T 17980.104-2004 pesticide field efficacy medicine test criterion (two) the 104th part: bactericidal agent for preventing and treating bakanae disease of rice > >.Trial crops is paddy rice, kind on educate 397, controlling object is bakanae disease of rice (Fusariummoniliforme).Test is carried out at Ba Litong town, Muling City one seed multiplication farm, and soil types is grassy marshland paddy soil, and the content of organic matter is that 3.2%, pH is 6.5.On April 20th, 2010, carry out seed treatment.Test medicine and dosage are: 200g/100 kilogram of seed of 10% SYP-3375 fludioxonil suspension seed-coating agent, 170g/100 kilogram seed, 140g/100 kilogram seed, 20% 150g/100 kilogram of SYP-3375 suspending agent seed, 2.5% 600g/100 kilogram of fludioxonil suspension seed-coating agent seed.Separately establish clear water blank, every processing repeats for 4 times, community, seedbed area 8 ㎡, and Honda community area 20 ㎡, random district group is arranged.Each chemicals treatment is processed the ratio of double centner seed in 1.6 hydrargyrum oxydatum crudum liquid, calculate dosing and the amount of being watered.Sowing after rice paddy seed is processed
Investigation and statistical method, investigate 2 times altogether, respectively at rice transplanting, rises before seedling and investigated before heading stage, and 5 points are investigated in every community, every 20 strains, and the diseased plant rate of community bakanae disease is respectively processed in investigation, calculates preventive effect.Each processes the impact on paddy rice at each crucial growing stage of paddy rice, to observe medicament.
Diseased plant rate (%)=(morbidity strain number/investigate total strain number) * 100
Control efficiency (%)=[(check plot diseased plant rate-treatment region diseased plant rate)/check plot diseased plant rate] * 100.
Field test results shows, 10% SYP-3375 fludioxonil suspension seed-coating agent has good control efficiency to bakanae disease of rice, every double centner seed is processed seed by 200 g, 170g, 140g consumption, in rice transplanting phase and heading stage, the preventive effect of bakanae disease of rice is all reached to 100%, be better than 150g/100 kilogram of seed of 20% SYP-3375 suspending agent, the preventive effect (table 6) of 600g/100 kilogram of seed treatment of 2.5% fludioxonil suspension seed-coating agent to bakanae disease of rice.
Before transplanting, seedling quality investigation shows, within the scope of 10% SYP-3375 fludioxonil suspension seed-coating agent test dose, process rice seedling rate higher than blank 2% left and right, the plant height of rice shoot, fresh weight are slightly better than blank, show that medicament is to paddy growth safety, without poisoning.
Result of the test has obviously improved the control efficiency to bakanae disease of rice after showing that SYP-3375 and fludioxonil are mixed, and has reduced dosage and drug cost, to paddy growth safety, is the desirable medicament of preventing and treating bakanae disease of rice.
In sum, composition of the present invention is to adopt two kinds of active component compound schemes, its activity and bactericidal effect are not the simple superposition of each composition activity, compare, except having significant bactericidal effect with existing unitary agent, and there is a significant synergistic effect, slow down the generation of resistance, preparation less residue, pollution-free to crop, safety is good, meets the security requirement of pesticidal preparations.
Claims (7)
1. containing a bactericidal composition for SYP-3375 and fludioxonil, it is characterized in that composition active component is SYP-3375 and fludioxonil, the weight ratio of SYP-3375 and fludioxonil is 1:20～3:1.
2. according to claim 1 containing the bactericidal composition of SYP-3375 and fludioxonil, it is characterized in that: the weight ratio of SYP-3375 and fludioxonil is 1:15～2:1.
3. according to claim 2 containing the bactericidal composition of SYP-3375 and fludioxonil, it is characterized in that: the weight ratio of SYP-3375 and fludioxonil is 1:10～1:1.
4. according to one of any described bactericidal composition containing SYP-3375 and fludioxonil of claims 1 to 3, it is characterized in that: the cumulant of SYP-3375 and fludioxonil is described composition total weight 1%～80%.
5. according to claim 1 containing the bactericidal composition of SYP-3375 and fludioxonil, it is characterized in that: the cumulant of SYP-3375 and fludioxonil is described composition total weight 5%～70%.
6. according to claim 1 containing the bactericidal composition of SYP-3375 and fludioxonil, it is characterized in that: described composition is wetting powder, suspending agent, water dispersible granules, suspension seed-coating agent.
7. described in claim 1, contain the bactericidal composition of SYP-3375 and fludioxonil in the purposes of preventing and treating in vegetables, fruit tree, Strawberry anthracnose, gray mold, bakanae disease of rice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110304798.5A CN102388888B (en) | 2011-10-11 | 2011-10-11 | Sterilization composite containing coumoxystrobin and fludioxonil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110304798.5A CN102388888B (en) | 2011-10-11 | 2011-10-11 | Sterilization composite containing coumoxystrobin and fludioxonil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102388888A CN102388888A (en) | 2012-03-28 |
| CN102388888B true CN102388888B (en) | 2014-03-19 |
Family
ID=45856385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110304798.5A Active CN102388888B (en) | 2011-10-11 | 2011-10-11 | Sterilization composite containing coumoxystrobin and fludioxonil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102388888B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935486A (en) * | 2014-03-11 | 2018-12-07 | 江苏龙灯化学有限公司 | A kind of bactericidal composition |
| CN105265434B (en) * | 2014-07-18 | 2018-03-23 | 中化农化有限公司 | Composition pesticide containing fludioxonil |
| CN106472609A (en) * | 2016-08-31 | 2017-03-08 | 江苏星源生物科技有限公司 | A kind of bactericidal composition preventing and treating root rotof flax and its application |
| CN107094766A (en) * | 2017-05-11 | 2017-08-29 | 深圳诺普信农化股份有限公司 | A kind of seed treatment |
| CN107668042A (en) * | 2017-11-20 | 2018-02-09 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition containing metconazole, fludioxonil and Metalaxyl-M |
| CN108684676A (en) * | 2018-07-07 | 2018-10-23 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition containing volution bacterium amine and fludioxonil |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1604738A (en) * | 2001-11-27 | 2005-04-06 | 辛根塔参与股份公司 | Synergistic seed treatment compositions |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010046387A2 (en) * | 2008-10-21 | 2010-04-29 | Basf Se | Use of a respiration complex iii inhibitor on cultivated plants |
-
2011
- 2011-10-11 CN CN201110304798.5A patent/CN102388888B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1604738A (en) * | 2001-11-27 | 2005-04-06 | 辛根塔参与股份公司 | Synergistic seed treatment compositions |
Non-Patent Citations (6)
| Title |
|---|
| Ai-YingGuan等.Design synthesis and structure–activity.《Pest Manag Sci》.2011 |
| Design, synthesis and structure–activity;Ai-Ying Guan 等;《Pest Manag Sci》;20110209(第67期);第647-655页 * |
| 丁香菌酯(SYP-3375)的设计、合成及杀菌活性;李淼 等;《农药学学报》;20101215;第12卷(第4期);全文 * |
| 李淼 等.丁香菌酯(SYP-3375)的设计、合成及杀菌活性.《农药学学报》.2010,第12卷(第4期),全文. |
| 甲氧丙烯酸酯类杀菌剂的研究进展;骆焱平 等;《中国科技论文在线》;20060815;第1卷(第1期);第22页 表1 * |
| 骆焱平 等.甲氧丙烯酸酯类杀菌剂的研究进展.《中国科技论文在线》.2006,第1卷(第1期),第22页 表1. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102388888A (en) | 2012-03-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103749522B (en) | The bactericidal composition of a kind of 2-cyano-3-amino-3-phenylancryic acetate-containing and acid amide fungicides | |
| CN102626099B (en) | Bactericidal composition containing cupric nonyl phenolsulfonate and methoxy-acrylate bactericide | |
| CN102388888B (en) | Sterilization composite containing coumoxystrobin and fludioxonil | |
| CN102696646B (en) | Sterilizing composite containing captan | |
| CN102715156B (en) | Sterilization composition containing js399-19 | |
| CN102640761B (en) | Sterilization composition containing SYP-1620 and oomycete prevention and treatment sterilization agents | |
| CN104839168A (en) | Fungicide composition and application thereof | |
| CN102428930B (en) | Bactericidal composition containing JS399-19 | |
| CN102388890B (en) | Bactericidal composition containing SYP-3375 and cyprodinil | |
| CN103749479B (en) | The bactericidal composition of a kind of 2-cyano-3-amino-3-phenylancryic acetate-containing and acid amide fungicides | |
| CN102919251B (en) | Bactericidal composition containing benthiavalicarb isopropyl and captan | |
| CN102835399B (en) | Metrafenone-containing bactericidal composition | |
| CN105076212B (en) | A kind of bactericidal composition containing bacillus subtilis and 2-cyano-3-amino-3-phenylancryic acetate | |
| CN102626097A (en) | Bactericidal composition containing famoxadone | |
| CN104604897A (en) | Sterilization composition containing thifluzamide and fludioxonil | |
| CN102696632B (en) | Bactericidal composition containing SYP-1620 | |
| CN102293215B (en) | Bactericidal composition containing coumoxystrobin and prochloraz | |
| CN102349531A (en) | Bactericidal composition containing coumoxystrobin and benzimidazole bactericide | |
| CN103271076B (en) | Bactericide composition containing SYP-1620 | |
| CN102630687B (en) | Bactericidal composition containing fenamistrobin and metiram | |
| CN103271032B (en) | Bactericide composition containing SYP-1620 | |
| CN102388875B (en) | Bactericidal composition containing coumoxystrobin and chlorothalonil | |
| CN103947649B (en) | A kind of composition pesticide containing glyoxalin and kasugarnycin | |
| CN103271036B (en) | Sterilization composition containing SYP-1620 | |
| CN102388891A (en) | Bactericidal composition containing SYP-3375 and ethirimol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170124 Address after: 136100 Gongzhuling national agricultural science and Technology Park in Jilin province Nan Wei Zi Zhen Jie Dong Patentee after: Jilin Bada Pesticide Co., Ltd. Address before: 9 No. 710404 Shaanxi province Xi'an Zhouzhi County of Jixian City Industrial Park Venture Road Patentee before: Shanggezhilu Biosciences Co., Ltd., Shaanxi |