CN108935486A - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
- Publication number
- CN108935486A CN108935486A CN201810623158.2A CN201810623158A CN108935486A CN 108935486 A CN108935486 A CN 108935486A CN 201810623158 A CN201810623158 A CN 201810623158A CN 108935486 A CN108935486 A CN 108935486A
- Authority
- CN
- China
- Prior art keywords
- fludioxonil
- prothioconazoles
- seed
- bactericidal composition
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bactericidal composition more particularly to a kind of bactericidal compositions containing prothioconazoles and fludioxonil；The invention further relates to a kind of methods for preventing and treating plant pathogen.The invention further relates to the diseases in storage after the harvest of control or prevention and treatment harvest fruit.A kind of bactericidal composition, containing prothioconazoles and fludioxonil, the weight proportion of prothioconazoles and fludioxonil is 1:100-100:1.The present invention is by carrying out binary built for prothioconazoles and fludioxonil, act on pathogenic bacteria and/or its environment, or can be used for preventing and treating cereal in plant, plant parts, seed, soil, region, material or space, fungi and bacterium in water fruits and vegetables, achieve the effect that synergy.And the bactericidal composition containing prothioconazoles and fludioxonil can be used for handling the seed for needing plant to be protected, it may also be used for the fruit of storage phase after processing harvest.
Description
The application is application No. is 201410086779.3, and the applying date is on March 11st, 2014, entitled " a kind of
The divisional application of bactericidal composition ".
Technical field
The present invention relates to a kind of bactericidal composition more particularly to a kind of Bactericidal composites containing prothioconazoles and fludioxonil
Object；The invention further relates to a kind of methods for preventing and treating plant pathogen.The invention further relates to control or the harvests of prevention and treatment harvest fruit
Disease in storage afterwards.
Background technique
Fungicide is natural or synthesis source compound, is used to protect plant not damage caused by by fungi.
Current agricultural methods are depended critically upon using fungicide.In fact, some crops cannot not use fungicide
In the case where effectively grow.The quality for using fungicide that grower is made to increase yield and crop, and thus increase and make
The value of object.In most cases, the value added of crop value is at least three times of cost using fungicide.
It but is all useful in all cases without a kind of fungicide, and it is antifungal to reuse single
Agent, which is frequently resulted in, generates drug resistance to this kind or relevant fungicide.Therefore, researching and producing it is safer, have more
Performance well, the composition for needing lower dosage, being easy use and expense lower fungicide and fungicide.
The object of the present invention is to provide there is the total amount of application for improving activity and reactive compound to reduce harmful fungoid
Composition (synergistic composition), to reduce rate of application and expand the activity profile of known compound.
Prothioconazoles are the triazole thioketone wide-spectrum bactericide that Beyer Co., Ltd develops, via W96/16048
Know.Prothioconazoles have good systemic activity, and activity is treated and rooted out in excellent protection, and the lasting period is long.A large amount of field
Pharmacodynamic test shows that prothioconazoles not only have good safety to crop, and preventing disease theraping effect is good, and increases production obvious.
Prothioconazoles (Prothioconazole) wheat disease has good control efficiency, white such as wheat and barley
Powder disease, banded sclerotial blight, wilt disease, leaf spot, rust, sclerotiniose, net blotch, moire disease etc..Prothioconazoles can also prevent and treat rape and
The soil-borne disease of peanut, such as sclerotiniose.Prothioconazoles can also prevent and treat main foliage disease, as gray mold, black spot, brown spot,
Balck shank, sclerotiniose and rust etc..Prothioconazoles single dose usage amount higher cost, long-time service can make germ develop drug resistance,
The control efficiency of disease on special crop will be reduced.
Prothioconazoles 2- [2- (1- chlorine cyclopropyl) -3-(2- chlorphenyl) -2- hydroxypropyl] -2,4- dihydro-[1,2,4] -
Triazole -3- thioketones, as known to WO96-16048.The compound can following formula " thioketones " form exist:
Or tautomerism " mercaptan " form of following formula exists:
For the sake of simplicity, " thioketones " form is only provided in each case.
Fludioxonil (Fludioxonil), chemical name: fluoro- 1, the 3- benzo dioxy -4- base of 4-(2,2- bis-) pyrroles's -3- nitrile,
Its molecular structural formula are as follows:
Fludioxonil is pyroles fungicide, via known to EP-A-206999.Fludioxonil is pseudomonas (Pseudomonas
Spp. the secondary metabolites pyrrolnitrin that (Pseudomonas pyrrocinia) not of the same race) is generated
(pyrrolnitrin) analog, due to its solution more anti-light than natural products pyrrolnitrin, mould can be inhibited in specific manner and
The fungal disease being widely used in prevention and treatment agricultural production.The antimicrobial mechanism of fludioxonil is then by inhibiting glucose phosphinylidyne
The related transfer changed, and inhibit the growth of fungal mycelium, eventually lead to the death of germ.The fludioxonil mechanism of action is unique, with
Existing fungicide no interactions resistance.In addition, fludioxonil avenges rotten Fusariumsp, the black corruption of wheat net raw meat for preventing and treating as foliage fungicide
Bacterium, rhizoctonia solani etc. have special efficacy to gray mold；As seed treatment: being mainly used for preventing and treating in cereal and non-cereals crop
Kind passes and soil-borne disease bacterium such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicillium bacterium
Deng.Fludioxonil is the whole world one of the products few in number for obtaining U.S.EPA EPA " low-risk " certification.
Due to now constantly improve to the environmental requirement of fungicide and economic requirement, such as to activity profile, toxicity, selection
Property, rate of application, residue composition and advantageous preparation feasibility requirement, the problem of in addition there are in terms of such as resistance, therefore,
Exploitation is in some aspects lasting task better than the new fungicide of existing fungicide.
Summary of the invention
We have found that being administered simultaneously, i.e., apply together or applying prothioconazoles and fludioxonil respectively, or successively apply rosickyite
Harmful fungoid preventive and therapeutic effect provided by bacterium azoles and fludioxonil is got well than the effect of each compound is administered alone.In other words, exist
Unpredictable, necessary being synergistic effect, rather than just active adduction.
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright
It is aobvious.But the weight ratio of the reactive compound in bactericidal composition can change in a certain range.
A kind of bactericidal composition of the present invention is to adopt the following technical solutions to achieve:
A kind of bactericidal composition of the present invention, it is characterised in that: it include prothioconazoles and fludioxonil, wherein prothioconazoles and fludioxonil
Weight proportion 1:100-100:1, preferably 1:50-50:1, more preferable 1:25-25:1, more preferable 1:10-10:1.
A kind of bactericidal composition, active constituent, filler and/or surfactant comprising prothioconazoles and fludioxonil.
The bactericidal composition dosage form is wettable powder, missible oil, suspending agent, microcapsules, seed coat agent, microemulsion, water
Emulsion, water dispersible granules, foaming agent, paste, aerosol, ultra-low volume spray dosage form.
The bactericidal composition, wherein the content of the active constituent containing prothioconazoles and fludioxonil accounts for bactericidal composition
5%-90%。
The bactericidal composition, wherein the content of the active constituent containing prothioconazoles and fludioxonil accounts for bactericidal composition
20%-80%。
A method of bactericidal composition is acted on pathogenic bacteria and/or its environment, Huo Zhezhi by prevention and treatment plant pathogen
In object, plant parts, seed, soil, region, material or space.
A method of prevention and treatment plant pathogen, prothioconazoles and fludioxonil are administered simultaneously or respectively application or in succession
Application.
The Bactericidal active ingredient composition or bactericidal composition is used to prevent and treat the purposes of fungi and bacterium.
The bactericidal composition be used to prevent and treat cereal, in water fruits and vegetables fungi and bacterium purposes.
In terms of the bactericidal composition is for protecting plant and protecting fruit storage phase does not break out disease after harvesting
Purposes.
Bactericidal composition of the invention, which has, effectively kills microbial activity, and can be in crop protection or material protection
For preventing and treating undesired microorganism, such as fungi and bacterium.
The plant appeared on plant or different useful plants can be prevented or destroyed using bactericidal composition of the invention
Microorganism on organ (fruit, flower, leaf, stem, stem tuber, root), thus after continued growth plant organ still not by this slightly
Biology infects.It applies or dresses seed after can also harvesting and use, or for handling plant propagation material, especially seed.
Bactericidal composition of the invention has extraordinary fungicide performance, and can be used for preventing and treating phytopathogenic fungi,
The fungi is especially selected from Plasmodiophoromycetes (Plasmodiophoromycetes), Oomycete (Oomycetes), Chytridiomycetes
(Chytridiomycetes), Zygomycetes (Zygomycetes), Ascomycetes (Ascomycetes), Basidiomycetes
(Basidiomycetes), Phycomycetes (Phycomycetes) and deuteromycetes (Deuteromycetes) fungi.
Bactericidal composition of the invention has extraordinary bactericidal performance, and can be used for preventing and treating phytopathogenic bacteria.
Such as pseudomonad (Psedomonadaceae), rhizobium (Rhizobiaceae), enterobacteria (Enterbacteriaceae), stick
Bacillus (Cornebacteriaceae) and streptomycete (Streptomycetaceae).
It can propose that being included into some of above-mentioned generic name below leads to fungal disease and bacterium in a non-limiting manner as example
The pathogen of disease:
Flavobacterium strain, such as the withered and yellow monad of rice blinds (Xanthomonascampestrispv.oryzae);
Pseudomonas strain, such as Bacterial leaf spot cucumber pvs oryzae and oryzicola (Pseudomonas
syringaepv.Lachrymans) ；
Erwinia strain, such as pears water epidemic disease Irving (family name) bacterium (Erwiniaamylovora)；
Rotten mould (Pythium) strain, such as Pythium ultimum (Pythium ultimum)；
Phytophthora (Phytophthora) strain, such as phytophthora infestans (Phytophthorainfestans)；
False downy mildew (Pseudoperonospora) strain, such as grass false downy mildew (Pseudoperonospora humuli) or ancient
Bar false downy mildew (Pseudoperonosporacubensis)；
Axis downy mildew (Plasmopara) strain, such as grape are raw axis downy mildew (Plasmoparaviticola)；
Disk downy mildew (Bremia) strain, such as lettuce disk downy mildew (Bremia lactucae)；
Downy mildew (Peronospora) strain, such as pea downy mildew (Peronospora pisi) or Cruciferae downy mildew
(P.brassicae)；
Powdery mildew (Erysiphe) strain, such as Erysiphe graminis (Erysiphe graminis)；
Sphaerotheca (Sphaerotheca) strain, such as balsamine list softgel shell (Sphaerothecafuliginea)；
Podosphaera (Podosphaera) strain, such as white cross hair list softgel shell (Podosphaeraleucotricha)；
Venturia (Venturia) strain, such as apple black star bacteria (Venturiainaequalis)；
Pyrenophora (Pyrenophora) strain, such as round nuclear cavity bacteria (Pyrenophorateres) or wheat nuclear cavity bacteria
(P.graminea) (conidial form: Drechslera, Syn: Helminthosporium)；
Cochliobolus category (Cochliobolus) strain, such as standing grain cochliobolus (Cochliobolussativus) (mitogenetic spore
Sub- form: Drechslera, Syn: Helminthosporium)；
Uromyces (Uromyces) strain, such as wart top uromyce (Uromycesappendiculatus)；
Handle rest fungus (Puccinia) strain, such as Puccinia recondita (Puccinia recondita)；
Sclerotinia (Sclerotinia) strain, such as Sclerotinia sclerotiorum (Sclerotiniasclerotiorum)；
Tilletia (Tilletia) strain, such as Tilletia caries (Tilletiacaries)；
Smut (Ustilago) strain, such as naked smut (Ustilago nuda) or oat smut (Ustilago
avenae) ；
Film lead fungi category (Pellicularia) strain, such as assistant help the wooden film lead fungi (Pelliculariasasakii)；Pears
Spore (Pyricularia) strain, such as Magnaporthe grisea (Pyricularia oryzae)；
Fusarium (Fusarium) strain, such as yellow fusarium (Fusarium culmorum)；
Botrytis (Botrytis) strain, such as Botrytis cinerea (Botrytis cinerea)；
Septoria (Septoria) strain, such as phaeosphaeria nodorum (Septoria nodorum)；
Leptosphaeria (Leptosphaeria) strain, such as Leptosphaeria nodorum；
Cercospora (Cercospora) strain, such as graying tail spore (Cercosporacanescens)；
Alternaria (Alternaria) strain, such as rape rod method (Alternariabrassicae)；
False Cercosporalla (Pseudocercosporella) strain, such as wheat Phyllostachys pubescens (Pseudocercosporella
herpotrichoides) ；
Phakopsora (Phakopsora) strain, such as Phakopsora pachyrhizi (Phakopsorapachyrhizi) and mountain horseleech layer
Rest fungus (Phakopsora meibomiae)；And
Rhizoctonia (Rhizoctonia) strain, such as Rhizoctonia solani Kuhn (Rhizoctoniasolani).
Bactericidal composition of the invention also has fabulous excitation in plant.Therefore, they apply also for adjusting
The internal system of defense of animals and plants resists the invasion of undesired microorganism.
In the present invention, plant excitation (resistance induction) compound should be understood such substance, they can swash
The system of defense of plant is sent out, thus, when after processed plant by undesired microbial inoculant, the plant performance goes out
Resistance significant to mentioned microorganism.
In the present invention, undesired microorganism should be understood phytopathogenic fungi and bacterium.Therefore, change of the invention
Closing object can be used for the invasion of pathogen described in protection plant resistant in certain time after treatment.Protective effect obtained
Time, which usually is from plant and is activated to close object and handled, continues 1 to 10 day, preferably 1 to 7 day.
Bactericidal active ingredient composition of the invention to various crops such as cotton, vegetable variety (such as cucumber, beans,
Tomato, potato and cucurbitaceous plant), barley, dogstail, oat, banana, coffee, corn, fruit variety, rice, rye, soybean,
A large amount of fungies are prevented and treated in grapevine, wheat, ornamental plant, peanut, rape, sugarcane and various seeds to be even more important.
Bactericidal active ingredient composition of the invention is particularly suitable for preventing and treating following plants pathogenic epiphyte: the standing grain in Cereal
Powdery mildew (Blumeria graminis) (powdery mildew), the two spore powdery mildews (Erysiphe in cucurbitaceous plant
Cichoracearum) and monofilament shell (Sphaerotheca fuliginea), the apple mildew bacterium in apple
(Podosphaera leucotricha), the grape snag shell mildew (Uncinula necator) in grapevine, Cereal
In handle rest fungus (Puccinia) belong to, the rhizoctonia (Rhizoctonia) in cotton, rice and lawn belongs to, in Cereal and sugarcane
Smut (Ustilago) belong to, the venturia inaequalis (Venturia inaequalis) (scab) in apple, Cereal
In the compacted spore of length (Helminthosporium) belong to, the wheat glume blotch bacterium (Septoria nodorum) in wheat, strawberry,
Botrytis cinerea (Botrytis cinerea) (gray mold) in vegetables, ornamental plant and grapevine, the peanut tail in peanut
Spore (Cercospora arachidicola), the eye spot bacterium (Pseudocercosporella in wheat and barley
Herpotrichoides), the Pyricularia oryzae in rice (Pyricularia oryzae), the phytophthora infestans in potato and tomato
(Phytophthora infestans), the grape single shaft in grapevine is mould (Plasmopara viticola), hops and
False downy mildew (Pseudoperonospora) in cucumber belongs to, and the rod method (Alternaria) in vegetables and fruit belongs to, in banana
Spherical cavity bacterium (Mycosphaerella) belong to and Neurospora (Fusarium) and wheel branch spore (Verticillium) category.
Bactericidal composition of the invention is particularly suitable for preventing and treating cereal disease such as powdery mildew, handle rest fungus and sickle-like bacteria,
It, can also be in dicotyledonous upper prevention and treatment powdery mildew with such as snag shell mildew of the disease in vinegrowing, uniaxial mildew and grape spore
With the cause of disease organic matter in downy mildew fungi and tikka.
Bactericidal composition of the invention can be used for preventing and treating wheat, barley, corn, rice, peanut, banded sclerotial blight on rape, black
Fringe disease, root rot, balck shank, sclerotiniose, full rot, bakanae disease multiple diseases.
Bactericidal composition of the invention has good plant tolerance under concentration needed for controlling plant diseases, this just makes
Can aerial part to plant, breed strain (propagationstock), seed and soil in vitro and handle.The present invention
Bactericidal composition can also foliage applying or be used for seed pelleting.The present invention also provides be coated with bactericidal composition of the invention
Seed.
Bactericidal composition of the invention is particularly suitable for the kind on the crops such as wheat, barley, corn, rice, peanut, rape
Attached bag clothing can effectively prevent the diseases such as banded sclerotial blight, smut, root rot, balck shank, sclerotiniose, full rot, the bakanae disease of crop
Evil.
The bactericidal composition shows reduced toxicity and excellent plant tolerance.All plants and plant portion
Position can be handled according to the present invention.The meaning of plant should be understood all plant and plant population in the present invention, such as
Wild plant need and unwanted or crop plants (crop plants including naturally occurring).Crop plants can be logical
It crosses conventional plant breeding and optimum seeking method or is obtained by biotechnology and genetic engineering method or by the combination of the method
Plant, including genetically modified plants also include the plant variety being weighed protection by plant seedling or not being protected by it.Plant parts
Meaning should be understood all grounds of plant and underground position and plant organ, such as bud, leaf, Hua Hegen, specifiable reality
Example has leaf, needle, stem, dry, flower, fructification, fruit, seed, root, stem tuber and rhizome.Plant parts further include harvested material, with
And asexual and sexual propagation object, such as rice shoot, stem tuber, rhizome, cutting and seed.
According to plant variety or plant cultivars, its planting site and growth conditions (soil, weather, vegetation period,
Nutrition), processing of the invention also can produce superadditivity (collaboration) effect.Thus the following effect more than actual desired can be obtained
Fruit, such as the amount of application of the present composition can be reduced and/or widen its action spectrum and/or improve its activity, improve plant
Upgrowth situation, improve high temperature or cold tolerance, raising to arid or to the tolerance of salt content in water or in soil, improve and open
Flower quality, make simpler harvesting, hasting of maturity, improve yield, improve the quality of harvested products and/or improve its nutritive value,
Improve the Storage property and/or its processing performance of harvested products.
According to the present invention, the processing carried out using Fungicidal compounds to plant and plant parts is straight according to conventional treatment method
Row is tapped into, or is realized by handling its local environment, habitat or storage space, the conventional treatment method such as immersion liquid, spray
Mist, atomizing, is broadcasted sowing, is smeared at evaporation, for propagation material, especially seed, can also carry out one or more layers coating.Use shape
Formula depends on desired purpose；Under any circumstance all it is ensured that inventive mixture finely and is evenly distributed as far as possible.It is excellent
The method of administration of choosing is to be administered to aboveground vegetation part, especially foliage applying；Application times and application rate depend on target
The biology and weather life condition of microorganism.However, active constituent can also reach plant by root system via soil or water
It is interior, thus location can be poured with liquid preparation or the substance is mixed in soil in solid form.Sterilization group of the invention
Close object can also be administered on seed-grain in seed treatment, thus stem tuber or cereal can be used the liquid preparation of every kind of active constituent according to
Secondary immersion, or wrapped up with the wet or dry preparation mixed.
In addition, bactericidal composition of the invention can also be used in safeguard industries material not by the invasion of fungi.Industrial materials packet
Include timber, paper, leather, building；Cooling system such as heating system, ventilating and air conditioning system etc..Composition of the invention can be prevented and treated
Adverse effect such as rots, fades or mouldy.
Bactericidal composition of the invention can after material, plant, harvest storage phase fruits and seeds by fungal infection it
It is preceding or apply later.
Prothioconazoles and fludioxonil are administered simultaneously or respectively application or sequential application.Prothioconazoles and fludioxonil are applied
Be not sequentially implement it is essential to the invention.
When using bactericidal composition of the invention, rate of application can change in a wider range according to the type of application.
Handle plant or harvest after fruit when, the rate of application for activating component composition is usually 0.1 to 10000g/
Ha, preferably 10 to 1000g/ha.
When handling seed, the rate of application of active ingredient compositions is usually 0.001 to 50g/kg seed, preferably 0.01
To 10g/kg seed.
Handle soil when, the rate of application of active ingredient compositions be usually 0.1 to 10000g/ha, preferably 1 to
5000g/ha。
Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to practical feelings when reality is applied
Condition and needs adjust rate of application especially in accordance with the seriousness of the property and germ of plant to be processed or crop.
Composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition at
Dividing can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair
Based on the dosage form of bright offer.
Bactericidal composition of the invention can be converted into conventional formulation, for example, wettable powder, missible oil, suspending agent, microcapsules,
Seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foaming agent, paste, aerosol, ultra-low volume spray dosage form.
A kind of bactericidal composition of the present invention includes reactive compound prothioconazoles and fludioxonil.The sterilization group
It closes in object, reactive compound prothioconazoles and fludioxonil are mixed with filler and/or surfactant.
According to the present invention, term " filler ", which refers to, can be combined or be combined with reactive compound to make it easier for being administered to
The natural or synthetic organic or inorganic compound of object (such as plant, crop or careless class).Therefore, the filler is preferably
It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.
In bactericidal composition of the present invention, the content containing prothioconazoles and fludioxonil reactive compound accounts for sterilization group
Close the 5%-90%, preferably 10%-80% of object.
These preparations can be produced by known method, for example, leading in the case where selectively using surfactant
It crosses and reactive compound is mixed with filler and prepares preparation.
Liquid filler is usual are as follows: water, alcohol type (such as methanol, ethyl alcohol, isopropanol, butanol, ethylene glycol etc.), ketone
(such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone etc.), ethers (such as ether, dioxanes, methylcellulose,
Tetrahydrofuran etc.), aliphatic hydrocarbon class (such as kerosene, mineral oil etc.), aromatic hydrocarbons class (such as benzene,
Toluene, dimethylbenzene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbon, chlorobenzene, etc.), halogenated hydrocarbon class, amide
Class, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal oil etc..
Solid-filling agent is usual are as follows: phyteral powdery type (such as soy meal, starch, grain dust, wood powder, bark powder, saw
End, walnut shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem particle, extract plant essence after residue
Deng), paper, sawdust, crush the synthesized polymer body of synthetic resin etc., clay class (such as kaolin, bentonite, acid china clay etc.),
Talcum powder class.Silica (such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates), active carbon, natural mineral matter class are (floating
Stone, attapulgite and zeolite etc.), fire diatomite, sand, (such as polyethylene, polypropylene, the polyvinylidene chloride such as plastics medium
Deng), potassium chloride, calcium carbonate, the inorganic mineral powder of calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, greening ammonium etc. chemistry
Fertilizer, clay fertilizer, these substances can be used alone or two or more is mixed.
To make the emulsification of effective component compound, dispersion, solubilized and/or wetting that surfactant example can be used
Fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol can such as be enumerated
Or the phosphate of phenol, the aliphatic ester of polyalcohol, alkyl aryl sulphonic acid, naphthalene sulfonic acid polymer, lignosulfonates, macromolecule comb
Branch shape copolymer, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and the epoxy of shape
The polyacrylates such as oxide condensate, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as
Based on individual vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine
Combination.
The natural of tackifier such as carboxymethyl cellulose and powder, particle or latex form and synthesis can be used in preparation
Polymer, such as gum arabic, polyvinyl alcohol and polyvinyl acetate or natural phospholipid, such as cephalin and lecithin, and
Synthetic phospholipid.Other additives are mineral oil and vegetable oil.
The colorant that may be used such as inorganic pigment, for example, iron oxide, titanium oxide and Prussian blue and organic dyestuff, such as
Alizarin dyes, azo dyes or metal phthalein mountain valley with clumps of trees and bamboo dyestuff and trace nutrient, such as iron, manganese, boron, ketone, cobalt, molybdenum and zinc salt.
The disintegrating agent that may be used is selected from: bentonite, urea, ammonium sulfate, aluminium chloride, citric acid, succinic acid, sodium bicarbonate
One of or it is a variety of.
The stabilizer that may be used is selected from: one of sodium citrate, resorcinol.
The antifreezing agent that may be used is selected from: one of ethylene glycol, propylene glycol, glycerine, urea are a variety of.
The defoaming agent is selected from: silicone oil, silicone compound, C10-20 Saturated fat acids compound, C8-10Fat
One of alcohol compound is a variety of.
Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent,
Stabilizer, screening agent.
The reactive compound can be mixed preparation with conventional additives by known way by the preparation of the invention.
The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersing agent, and/or adhesive or fixative,
Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, defoaming agent, preservative, increasing
Thick dose, water and other processing aids.
These compositions not only include can or dusting equipment such as spraying by suitable equipment be suitable for immediately it is to be processed
Object, but also including needing the concentration commercial composition being diluted before being applied to object.
Seed dressing is to apply bactericidal composition of the invention in a manner of originally known with suitable seed dressing formulation form example
Such as there is the water slurry of good adhesion or dry powder form to be administered on seed seed.The seed dressing be this field
Know.The composition of single active constituent or active constituent of the seed dressing containing encapsulated forms, such as spansule or micro-
Capsule.
Active ingredient compositions of the invention can also be applied with other active ingredient combinations, such as expanding activity profile
Or it prevents from forming resistance.Other active constituents for example fungicide, bactericide, attractant, insecticide, acaricide,
Nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..
A kind of bactericidal composition of the present invention, including prothioconazoles and fludioxonil reactive compound, have synergistic function,
The bactericidal activity of bactericidal composition of the invention is more considerably higher than the active adduction of single reactive compound.In other words, exist
Unpredictable, necessary being synergistic effect, rather than just active supplement.
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright
It is aobvious.
The good bactericidal property of bactericidal composition of the invention can be illustrated by the following examples.Although individually sterilization
Reactive compound activity in terms of its bactericidal effect is weaker, but bactericidal composition exhibits more than simply summing it up for each self-applying
Activity.
Specific embodiment
Below with reference to embodiment, the invention will be further elaborated.
Example of formulations
+ 10% fludioxonil wettable powder of 1 2% prothioconazoles of embodiment
Prothioconazoles 2%
Fludioxonil 10%
Lauryl sodium sulfate 10%
Sodium lignin sulfonate 5%
Kaolin complements to 100%
Active constituent, various auxiliary agents and filler etc. are mixed by the proportional components of formula, arrived after ultra-fine pulverizer disintegrating
+ 10% fludioxonil wettable powder of 2% prothioconazoles.
+ 50% fludioxonil wettable powder of 2 1% prothioconazoles of embodiment
Prothioconazoles 1%
Fludioxonil 50%
Calcium dodecyl benzene sulfonate 1%
Sodium lignin sulfonate 2%
Attapulgite complements to 100%
Active constituent, various auxiliary agents and filler etc. are mixed by the proportional components of formula, to get to 1% rosickyite after ultra-fine grinding
+ 50% fludioxonil wettable powder of bacterium azoles.
+ 4% fludioxonil missible oil of 3 1% prothioconazoles of embodiment
Prothioconazoles 1%
Fludioxonil 4%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
N-Methyl pyrrolidone complements to 100%
Mentioned component is proportionally prepared, uniform phase is uniformly mixing to obtain.
+ 50% fludioxonil wettable powder of 4 5% prothioconazoles of embodiment
Prothioconazoles 5%
Fludioxonil 50%
Lauryl sodium sulfate 10%
Sodium lignin sulfonate 5%
White carbon black complements to 100%
Said components are mixed in proportion, and grinds, crush, are prepared into wettable powder.
+ 50% fludioxonil water dispersible granules of 5 2% prothioconazoles of embodiment
Prothioconazoles 2%
Fludioxonil 50%
MODIFIED LIGNOSULPHONATE 5%
Lauryl sodium sulfate 5%
Urea 5%
Kaolin complements to 100%
Prothioconazoles active constituent, dispersing agent, wetting agent, disintegrating agent and filler are mixed according to the formula uniformly, by gas
Stream is ground into wettable powder；Fludioxonil is added to be uniformly mixed；It adds a certain amount of water mixing extruding and makes material.Through drying
+ 50% fludioxonil water dispersible granules of 2% prothioconazoles are obtained after screening.
+ 50% fludioxonil suspoemulsion of 6 0.5% prothioconazoles of embodiment
Oily phase:
Prothioconazoles 0.5%
Methyl oleate 10%
Ethoxylated castor oil 5%
Water phase:
Fludioxonil 50%
Naphthalene sulfonic acids-formaldehyde condensation products sodium salt 1% of sulfonation
Water complements to 100%
Prothioconazoles are dissolved in methyl oleate, ethoxylated castor oil is added and obtains oily phase；According to formula by fludioxonil, sulphur
The aqueous suspension agent of fludioxonil is obtained after naphthalene sulfonic acids-formaldehyde condensation products sodium salt, the water of change is ground and/or high speed shear；?
The oil phase is added to the aqueous phase under stirring obtains suspoemulsion.
+ 10% fludioxonil wettable powder of 7 10% prothioconazoles of embodiment
Prothioconazoles 10%
Fludioxonil 10%
Sodium lignin sulfonate 1%
NaLS 2%
The silicic acid 1% of high degree of dispersion
Kaolin complements to 100%
Said components are mixed in proportion, and grinds, crush, are prepared into wettable powder.
+ 50% fludioxonil coated granule of 8 40% prothioconazoles of embodiment
Prothioconazoles 40%
Fludioxonil 50%
Polyethylene glycol 3%
The silicic acid 1% of high degree of dispersion
Calcium carbonate complements to 100%
It in a mixer, will be on levigate active constituent even spread to the carrier soaked by polyethylene glycol.It can obtain by this method
Obtain dust-free coated granules agent.
+ 10% fludioxonil wettable powder of 9 50% prothioconazoles of embodiment
Prothioconazoles 50%
Fludioxonil 10%
Lauryl sodium sulfate 1%
Sodium lignin sulfonate 1%
Kaolin complements to 100%
Said components are mixed in proportion, and grinds, crush, are prepared into wettable powder.
+ 60% fludioxonil of 10 20% prothioconazoles of embodiment squeezes out granule
Prothioconazoles 20%
Fludioxonil 60%
Sodium lignin sulfonate 4%
Carboxymethyl cellulose 2%
Kaolin complements to 100%
Active component is mixed and ground with auxiliary agent, mixture is soaked with water.The mixture is squeezed out, is then done in the air stream
It is dry.
11 50% prothioconazoles of embodiment++ 2% fludioxonil suspension seed-coating agent
Prothioconazoles 50%
Fludioxonil 2%
Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 10%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Hydroxyethyl cellulose 1%
Glycerine 5%
PVP-K30 1%
Water complements to 100%
Seed coat agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.
+ 5% fludioxonil microcapsule suspension-suspendinagent agent of 12 50% prothioconazoles of embodiment
ATLOXTM4913 4%
Citric acid 0.05%
Catalyst 0.1%
Water 13%
Fludioxonil 5%
Polyphenyl polymethylene polyisocyanates 1.35%
SOLVESSOTM200 10%
ATLOXTM4913 16%
Dispersing agent LFH 0.3%
Defoaming agent 0.16%
Urea 8.4%
Prothioconazoles 50%
Water complements to 100%
By polyphenyl polymethylene polyisocyanic acid (PAPI), prothioconazoles, SOLVESSOTM200 oil formed, which are added to, to be contained
ATLOX TMIn 4913 aqueous solution, emulsion is formed.Then it heats and keeps the temperature 50oIt is added catalyst reaction 2 hours under C.
The microcapsules of prothioconazoles is obtained after cooling.
ATLOX TM4913, dispersing agent LFH, defoaming agent, urea, fludioxonil and water mix in proportion, it is ground and/or
It obtains uniformly, obtaining suspending agent after high speed shear.
Will obtained prothioconazoles microcapsules be added fludioxonil suspending agent in, be uniformly mixing to obtain 50% prothioconazoles+
5% fludioxonil microcapsule suspension-suspendinagent agent.
+ 1% fludioxonil suspending agent of 13 50% prothioconazoles of embodiment
Prothioconazoles 50%
Fludioxonil 1%
| Methyl naphthalene sulfonate formaldehyde condensate | 5% |
| Dioctyl succinate disulfonate acid | 5% |
| Xanthan gum | 0.3% |
| Glycerine | 5% |
| Defoaming agent | 0.6% |
| Water | Complement to 100% |
The each components such as active component, dispersing agent, wetting agent and water are uniformly mixed according to the ratio of formula, it is ground and/or high
Speed shearing controls partial size at 5 μm hereinafter, obtaining+1% fludioxonil suspending agent of 50% prothioconazoles.
+ 0.5% fludioxonil missible oil of 14 50% prothioconazoles of embodiment
Prothioconazoles 50%
Fludioxonil 0.5%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
SOLVESSOTM100 complement to 100%
Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.
+ 30% fludioxonil water dispersible granules of 15 20% prothioconazoles of embodiment
Prothioconazoles 20%
Fludioxonil 30%
MODIFIED LIGNOSULPHONATE 5%
Lauryl sodium sulfate 5%
Ammonium sulfate 2%
Kaolin complements to 100%
Prothioconazoles, fludioxonil active constituent, dispersing agent, wetting agent, disintegrating agent and filler are mixed according to the formula uniformly,
By air-flow crushing at wettable powder；It adds a certain amount of water mixing extruding and makes material.20% rosickyite is obtained after dry screening
+ 30% fludioxonil water dispersible granules of bacterium azoles.
+ 30% fludioxonil wettable powder of 16 5% prothioconazoles of embodiment
Prothioconazoles 5%
Fludioxonil 30%
Lauryl sodium sulfate 1%
Sodium lignin sulfonate 1%
Kaolin complements to 100%
Said components are mixed in proportion, and grinds, crush, are prepared into wettable powder.
+ 10% fludioxonil aqueous emulsion of 17 1% prothioconazoles of embodiment
Oily phase:
| Prothioconazoles | 1% |
| Fludioxonil | 10% |
| Methyl oleate | 10% |
| Polystyrene | 3.7% |
Water phase:
| Xanthan gum | 0.07% |
| Naphthalene sulfonic acids-formaldehyde condensation products sodium salt of sulfonation | 1% |
| Fungicide | 0.2% |
| Water | Complement to 100% |
Prothioconazoles and fludioxonil are dissolved in methyl oleate, polystyrene is added and obtains oily phase；According to the component in formula
It is uniformly mixed and obtains water phase；The oil phase is added to the aqueous phase under stiring obtains aqueous emulsion.
+ 8% fludioxonil microemulsion of 18 10% prothioconazoles of embodiment
Prothioconazoles 10%
Fludioxonil 8%
Ethoxylated castor oil 10%
Calcium dodecyl benzene sulfonate 10%
SOLVESSOTM100 50%
Water complements to 100%
Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.
+ 60% fludioxonil of 19 40% prothioconazoles of embodiment
Prothioconazoles 40%
Fludioxonil 60%
Prothioconazoles, fludioxonil are proportionally uniformly mixed.
+ 50% fludioxonil of 20 50% prothioconazoles of embodiment
Prothioconazoles 50%
Fludioxonil 50%
Proportion in above embodiments is weight per distribution ratio.
Biological Examples
Test one: prothioconazoles and fludioxonil compound the indoor toxicity test to piricularia oryzae
Experiment target is designated as piricularia oryzae, and test is acted on using the co-toxicity of co-toxicity coefficient method evaluation compound preparation.
Prothioconazoles and fludioxonil are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture medium is made in the fludioxonil of different ratio and prothioconazoles series of concentrations compound solution with same method.
2 days piricularia oryzae will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, will be moved to fungus block with transfer needle pre-
The toxic PDA culture medium center being first configured to is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.After 3 days, use
Crossing method measures each processing colony diameter with slide calliper rule, and unit is millimeter.It finds out correction and inhibits percentage.Each bacterium colony cross
Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
The EC that will be managed everywhere in measurement according to Sun Yunpei (Y-P Sun) method50It is converted into practical poison exponent (ATI)；According to mixture
Proportion, calculate theoretical toxicity index (TTI), the co-toxicity coefficient (CTC) of mixture is calculated according to the following formula.
Here AM refers to ingredient A shared weight percent in the composition, and BM refers to ingredient B shared weight in the composition
Measure percentage.
If co-toxicity coefficient is greater than 120, show there is synergistic effect；If being lower than 100, it is shown to be antagonism；Between 100 ~ 120,
It is shown to be summation action.
The results are shown in Table 1 for the toxicity test of this test one:
Table 1
| Medicament | Proportion | EC50(mg/L) | ATI | TTI | CTC |
| Prothioconazoles | - | 6.43 | 100 | / | / |
| Fludioxonil | - | 9.40 | 68.4 | / | / |
| Prothioconazoles+fludioxonil | 100:1 | 4.82 | 133.28 | 99.69 | 133.7 |
| Prothioconazoles+fludioxonil | 50:1 | 4.07 | 157.92 | 99.38 | 158.9 |
| Prothioconazoles+fludioxonil | 25:1 | 3.31 | 194.01 | 98.78 | 196.4 |
| Prothioconazoles+fludioxonil | 10:1 | 3.27 | 196.68 | 97.13 | 202.5 |
| Prothioconazoles+fludioxonil | 5:1 | 3.42 | 188.05 | 94.73 | 210.5 |
| Prothioconazoles+fludioxonil | 1:1 | 4.14 | 155.18 | 84.20 | 198.5 |
| Prothioconazoles+fludioxonil | 1:5 | 4.74 | 135.77 | 73.67 | 184.3 |
| Prothioconazoles+fludioxonil | 1:10 | 5.21 | 123.44 | 71.27 | 173.2 |
| Prothioconazoles+fludioxonil | 1:25 | 5.63 | 114.31 | 69.62 | 164.2 |
| Prothioconazoles+fludioxonil | 1:50 | 6.04 | 106.50 | 69.02 | 154.3 |
| Prothioconazoles+fludioxonil | 1:100 | 6.53 | 98.40 | 68.71 | 143.2 |
It can be seen that from the data of table 1 when the proportion of prothioconazoles and fludioxonil is within the scope of 1:100 ~ 100:1, altogether poison system
Number is all larger than 120, it can be seen that the compound drug of the two achieves the effect of gain on prevention and treatment piricularia oryzae, especially
When the proportion of prothioconazoles and fludioxonil is when 5:1 range, gain effect is especially prominent.
Experiment two: the indoor toxicity test of prothioconazoles and fludioxonil to wheat smut
Experiment target is designated as wheat smut, and test is acted on using the co-toxicity of co-toxicity coefficient method evaluation compound preparation.
Prothioconazoles and fludioxonil are used into acetone solution respectively, then are configured to 0.1% Tween-80 aqueous solution dilution
The medical fluid of series of concentrations draws 6mL to the conical flask of sterilizing respectively in superclean bench, 50 DEG C or so of potato is added
Glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic training of 4 respective concentrations is made
Support base；Toxic culture medium is made in the fludioxonil of different ratio and prothioconazoles series of concentrations compound solution with same method.
2 days wheat smut pathogens will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium
Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3
After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross
Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
The EC that will be managed everywhere in measurement according to Sun Yunpei (Y-P Sun) method50It is converted into practical poison exponent (ATI)；According to mixture
Proportion, calculate theoretical toxicity index (TTI), the co-toxicity coefficient (CTC) of mixture is calculated according to the following formula.
Here AM refers to ingredient A shared weight percent in the composition, and BM refers to ingredient B shared weight in the composition
Measure percentage.
If co-toxicity coefficient is greater than 120, show there is synergistic effect；If being lower than 100, it is shown to be antagonism；Between 100 ~ 120,
It is shown to be summation action.
To the The Toxicity Determination result of wheat smut, as shown in table 2:
Table 2
| Medicament | Proportion | EC50(mg/L) | ATI | TTI | CTC |
| Prothioconazoles | - | 0.86 | 100 | / | / |
| Fludioxonil | - | 1.54 | 55.8 | / | / |
| Prothioconazoles+fludioxonil | 100:1 | 0.60 | 143.07 | 99.56 | 143.7 |
| Prothioconazoles+fludioxonil | 50:1 | 0.49 | 173.78 | 99.13 | 175.3 |
| Prothioconazoles+fludioxonil | 25:1 | 0.47 | 183.23 | 98.30 | 186.4 |
| Prothioconazoles+fludioxonil | 10:1 | 0.43 | 202.04 | 95.98 | 210.5 |
| Prothioconazoles+fludioxonil | 5:1 | 0.34 | 256.04 | 92.63 | 234.5 |
| Prothioconazoles+fludioxonil | 1:1 | 0.43 | 200.83 | 77.90 | 276.4 |
| Prothioconazoles+fludioxonil | 1:5 | 0.53 | 162.84 | 63.17 | 257.8 |
| Prothioconazoles+fludioxonil | 1:10 | 0.58 | 147.21 | 59.82 | 246.1 |
| Prothioconazoles+fludioxonil | 1:25 | 0.69 | 125.06 | 57.50 | 217.5 |
| Prothioconazoles+fludioxonil | 1:50 | 0.91 | 94.86 | 56.67 | 167.4 |
| Prothioconazoles+fludioxonil | 1:100 | 1.02 | 83.91 | 56.24 | 149.2 |
It can be seen that from the data of table 2 when the proportion of prothioconazoles and fludioxonil is within the scope of 1:100 ~ 100:1, altogether poison system
Number is all larger than 120, it can be seen that the compound drug of the two achieves the effect of gain in prevention and treatment wheat smut, especially
It is when the proportion of prothioconazoles and fludioxonil is within the scope of 1:1, gain effect is especially prominent.
Biological test example
When the effect of active compound combinations is more than the summation of the effect when each reactive compound is administered alone, there is association
Same synergistic effect.The predictive role of the specific combination of two kinds of reactive compounds can be used so-called " Colby formula " (referring to
S.R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide
Combinations ", Weeds 1967,15,20-22) and it calculates as follows: if
X is the activity when the use of dosage being mg/ha or reactive compound A that concentration is mppm；
Y is the activity when the use of dosage being ng/ha or reactive compound B that concentration is nppm, and it is untreated right to be expressed as accounting for
According to percentage；
E is the activity when the use of dosage being m and n g/ha or reactive compound A and B that concentration is m and n ppm,
So
If the activity (O) of actual observation is greater than expection activity (E), the super adduction of the composition has synergistic effect.
Following biological test example is to illustrate the present invention.But invention is not limited to these embodiments.
Test 1 powdery mildew test (wheat)/protection activity
Solvent: the N- methyl-pyrrolidon of 10 parts of weight
Emulsifier: the alkylaryl polyglycol ether of 0.6 part of weight
In order to which suitable reactive compound is made, by the combination of a reactive compound or reactive compound by weight with it is upper
The solvent and emulsifier for the amount of stating mix, and are diluted with water to required concentration.
In order to measure protection activity, seedling is sprayed with the active agent preparations of the dosage.Liquid layer to be painted becomes
After dry, the spore of seedling wheat powdery mildew is dusted.Then test plant is placed in temperature about 20OC, air humidity about 80%
Promote the growth of white powder scab in greenhouse.Inoculation carried out effect assessment after 7 days, and 0% refers to that effect is suitable with what is compareed, and 100%
Refer to be not observed and infect.Reactive compound, dosage and test result are listed in the following table:
Test 2 powdery mildews test (wheat)/therapeutic activity
Solvent: the N- methyl-pyrrolidon of 10 parts of weight
Emulsifier: the alkylaryl polyglycol ether of 0.6 part of weight
In order to which suitable reactive compound is made, by the combination of a reactive compound or reactive compound by weight with it is upper
The solvent and emulsifier for the amount of stating mix, and are diluted with water to required concentration.
In order to test therapeutical active, seedling is dusted with wheat powdery mildew spore.After inoculation 48 hours, by seedling institute
The active agent preparations for stating dosage are spraying.Then test plant is placed in temperature about 20OC, the temperature of air humidity about 80%
Promote the growth of white powder scab in room.Inoculation carried out effect assessment after 7 days, and 0% refers to that effect is suitable with what is compareed, and 100%
Refer to be not observed and infect.Reactive compound, dosage and test result are listed in the following table:
Test 3 sclerotinioses test (rape)/seed treatment
Reactive compound is with the application of dry seeds seed dressing.Said preparation can be by by individual reactive compound or combined activation
It closes object to be mixed with levigate minerals to prepare, obtaining can be in the equally distributed finely powdered mixt of the surface of the seed.
When seed is dressed seed, seed and seed seed dressing will be infected and shaken 3 minutes in closed glass flask.Rape seed
Son is seeded in the standard soil of 1 centimetre of depth, in temperature about 18OC is cultivated in the greenhouse of air humidity about 95%, seeds cultivation
Case is per illumination in 15 hours day by day.
After sowing 3 weeks, the illness of Brassica campestris L seedling is evaluated, 0% refers to that effect is suitable with what is compareed, and 100% refers to and is not observed
It infects.Reactive compound, dosage and test result are listed in the following table:
Test 4 seedling stage base rot diseases (corn)/seed treatment
Reactive compound is with the application of dry seeds seed dressing.Said preparation can be by by individual reactive compound or combined activation
It closes object to be mixed with levigate minerals to prepare, obtaining can be in the equally distributed finely powdered mixt of the surface of the seed.
When seed is dressed seed, seed and seed seed dressing will be infected and shaken 3 minutes in closed glass flask.Maize seed
Son is seeded in the standard soil of 1 centimetre of depth, in temperature about 18OC is cultivated in the greenhouse of air humidity about 95%, seeds cultivation
Case is per illumination in 15 hours day by day.
After sowing 3 weeks, the illness of corn is evaluated, 0% refers to that effect is suitable with what is compareed, and 100% refers to be not observed and invades
Dye.Reactive compound, dosage and test result are listed in the following table:
Test 5 ash arrhizus bacterias test (strawberry)/therapeutic activity
Solvent: the N- methyl-pyrrolidon of 10 parts of weight
Emulsifier: the alkylaryl polyglycol ether of 0.6 part of weight
In order to which suitable reactive compound is made, by the combination of a reactive compound or reactive compound by weight with it is upper
The solvent and emulsifier for the amount of stating mix, and are diluted with water to required concentration.
In order to test therapeutical active, seedling is dusted with Botrytis cinerea bacterium spore.After inoculation 48 hours, by Strawberry seedlings
It is spraying with the active agent preparations of the dosage.Then test plant is placed in temperature about 20OC, air humidity about 80%
Greenhouse in promote the growth of grey mold scab.Inoculation carried out effect assessment after 7 days, and 0% refers to that effect is suitable with what is compareed,
100% refers to be not observed and infects.Reactive compound, dosage and test result are listed in the following table:
Test 6 anthracnose test (capsicum)/therapeutic activity
Solvent: the N- methyl-pyrrolidon of 10 parts of weight
Emulsifier: the alkylaryl polyglycol ether of 0.6 part of weight
In order to which suitable reactive compound is made, by the combination of a reactive compound or reactive compound by weight with it is upper
The solvent and emulsifier for the amount of stating mix, and are diluted with water to required concentration.
In order to test therapeutical active, seedling is dusted with pepper anthracnose spore.After inoculation 48 hours, by pepper seedling
It is spraying with the active agent preparations of the dosage.Then test plant is placed in temperature about 20OC, air humidity about 80%
Greenhouse in promote the growth of anthracnose.Inoculation carried out effect assessment after 7 days, and 0% refers to that effect is suitable with what is compareed, and 100%
Refer to be not observed and infect.Reactive compound, dosage and test result are listed in the following table:
Test 7 rhizoctonia tests/soil treatment
Mixing sows the seed of 7 cucumber by after the medicament of rhizoctonia contaminated soil and specified amount in plastic tub.Sowing is opened
After beginning 66 days, effect assessment is carried out.0% refers to that effect is suitable with what is compareed, and 100% refers to be not observed and infects.Activation
Object, dosage and test result is closed to be listed in the following table:
Test 8 smut test (wheat)/protection activity
Solvent: the N- methyl-pyrrolidon of 10 parts of weight
Emulsifier: the alkylaryl polyglycol ether of 0.6 part of weight
In order to which suitable reactive compound is made, by the combination of a reactive compound or reactive compound by weight with it is upper
The solvent and emulsifier for the amount of stating mix, and are diluted with water to required concentration.
In order to measure protection activity, seedling is sprayed with the active agent preparations of the dosage.Liquid layer to be painted becomes
After dry, seedling is dusted with wheat smut bacterium spore.Then test plant is placed in temperature about 20OC, air humidity about 80%
Promote the growth of black tassel bacteria in greenhouse.Inoculation carried out effect assessment after 7 days, and 0% refers to that effect is suitable with what is compareed, and 100%
Refer to be not observed and infect.Reactive compound, dosage and test result are listed in the following table:
Test 9 Magnaporthe griseas test (rice)/protection activity
In order to which suitable reactive compound is made, by the combination of a reactive compound or reactive compound by weight with it is upper
The solvent and emulsifier for the amount of stating mix, and are diluted with water to required concentration.
In order to measure protection activity, rice seedling is sprayed with the active agent preparations of the dosage.To hydrojet
After layer dries out, the spore of seedling Magnaporthe grisea is dusted.Then test plant is placed in temperature about 20OC, air humidity about 80%
Promote the growth of Magnaporthe grisea in greenhouse.Inoculation carried out effect assessment after 5 days, and 0% refers to that effect is suitable with what is compareed, and 100%
Refer to be not observed and infect.Reactive compound, dosage and test result are listed in the following table:
Test 10: to the effect of Botrytis cinerea on apple
3 holes are bored on apple, and the test composition that 50ul is prepared is instilled in each hole.2 were administered as a child, by 50ul ash Portugal
The spore suspension of grape spore is moved on on application position with liquid relief gun barrel.20OIt is incubated for 5 days, determines infected in cultivating chamber under C
Fruit area %.Relative to the disease rate calculating effect on untreated apple.
In terms of the test result of above 1~test of test 10, the preventive effect observed after the compounding of prothioconazoles and fludioxonil is obvious
Higher than preventive effect is calculated, apparent synergistic function is shown.
Claims (11)
1. a kind of bactericidal composition is used to prevent and treat the purposes of Maize stern rot, sclerotinia sclerotiorum, pepper anthracnose, special
Sign is that the bactericidal composition includes prothioconazoles and fludioxonil reactive compound, the weight ratio of prothioconazoles and fludioxonil
For 1:50-50:1.
2. the weight ratio of purposes according to claim 1, the prothioconazoles and fludioxonil is 1:25-25:1.
3. the weight ratio of purposes according to claim 1, the prothioconazoles and fludioxonil is 1:10-10:1.
4. the weight ratio of purposes according to claim 1, the prothioconazoles and fludioxonil is 1:5-5:1.
5. purposes according to claim 1, the bactericidal composition includes prothioconazoles and fludioxonil reactive compound,
Filler and/or surfactant.
6. purposes according to claim 5, which is characterized in that the bactericidal composition, dosage form are wettable powder, cream
Oil, suspending agent, microcapsules, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foaming agent, paste, aerosol, ultra-low volume
Spray.
7. purposes according to claim 1, which is characterized in that being handled with the bactericidal composition described in claim 1 needs
The seed of plant to be protected.
8. purposes according to claim 7, the seed of the plant is corn seed, rape seed, pepper seed.
9. a kind of method of prevention and treatment Maize stern rot, sclerotinia sclerotiorum, pepper anthracnose, which is characterized in that by right
It is required that bactericidal composition described in 1 acts on pathogenic bacteria and/or its environment or plant, seed, soil.
10. according to the method described in claim 9, being needed it is characterized in that, being handled with bactericidal composition described in claim 1
The seed of protection.
11. according to the method described in claim 9, it is characterized in that, by prothioconazoles described in claim 1 and fludioxonil
Reactive compound is administered simultaneously or sequential application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810623158.2A CN108935486A (en) | 2014-03-11 | 2014-03-11 | A kind of bactericidal composition |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410086779.3A CN104904723A (en) | 2014-03-11 | 2014-03-11 | Sterilization composition |
| CN201810623158.2A CN108935486A (en) | 2014-03-11 | 2014-03-11 | A kind of bactericidal composition |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410086779.3A Division CN104904723A (en) | 2014-03-11 | 2014-03-11 | Sterilization composition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108935486A true CN108935486A (en) | 2018-12-07 |
Family
ID=54070915
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410086779.3A Pending CN104904723A (en) | 2014-03-11 | 2014-03-11 | Sterilization composition |
| CN201810623158.2A Withdrawn CN108935486A (en) | 2014-03-11 | 2014-03-11 | A kind of bactericidal composition |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410086779.3A Pending CN104904723A (en) | 2014-03-11 | 2014-03-11 | Sterilization composition |
Country Status (2)
| Country | Link |
|---|---|
| CN (2) | CN104904723A (en) |
| WO (1) | WO2015135421A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113057172A (en) * | 2021-02-22 | 2021-07-02 | 安徽润农腾辉生物科技有限公司 | Ternary compound seed dressing agent containing prothioconazole and fludioxonil |
| CN115152773A (en) * | 2022-06-17 | 2022-10-11 | 安徽久易农业股份有限公司 | Composition of prothioconazole and fludioxonil for preventing and treating basal stem rot of wheat |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417311A (en) * | 2015-08-12 | 2017-02-22 | 四川利尔作物科学有限公司 | Seed treating agent composition and application thereof |
| CN107788001A (en) * | 2016-08-29 | 2018-03-13 | 四川利尔作物科学有限公司 | Seed treatment composition and its application |
| CN109511661A (en) * | 2017-09-20 | 2019-03-26 | 佛山市盈辉作物科学有限公司 | Containing prothioconazoles, fludioxonil, fluorine pyrrole furanone compounding seed treatment |
| CN109042700A (en) * | 2018-08-20 | 2018-12-21 | 贵州道元生物技术有限公司 | A kind of combination sterilization composition for preventing and treating grape grey mould |
| CN110376341B (en) * | 2019-06-24 | 2022-04-15 | 广西大学 | Method for determining photolysis of tomato gray mold control drug |
| AU2021221818A1 (en) * | 2021-08-25 | 2023-03-16 | Adama Australia Pty Limited | Highly Loaded Fludioxonil Formulations |
| CN115349322A (en) * | 2022-10-21 | 2022-11-18 | 海南大学三亚南繁研究院 | Seed treatment method for preventing and controlling soybean seed-borne diseases |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1642423A (en) * | 2002-03-21 | 2005-07-20 | 巴斯福股份公司 | Fungicidal mixtures |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19716257A1 (en) * | 1997-04-18 | 1998-10-22 | Bayer Ag | Fungicidal active ingredient combination |
| CN102388888B (en) * | 2011-10-11 | 2014-03-19 | 陕西上格之路生物科学有限公司 | Sterilization composite containing coumoxystrobin and fludioxonil |
| CN102696648A (en) * | 2012-04-23 | 2012-10-03 | 广东中迅农科股份有限公司 | Pesticidal composition comprising prothioconazole and thiram |
-
2014
- 2014-03-11 CN CN201410086779.3A patent/CN104904723A/en active Pending
- 2014-03-11 CN CN201810623158.2A patent/CN108935486A/en not_active Withdrawn
-
2015
- 2015-02-26 WO PCT/CN2015/073290 patent/WO2015135421A1/en active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1642423A (en) * | 2002-03-21 | 2005-07-20 | 巴斯福股份公司 | Fungicidal mixtures |
Non-Patent Citations (2)
| Title |
|---|
| 李碧霞: "《农药实用技术》", 31 July 2013, 阳光出版社 * |
| 杨青云: "《农药安全使用技术》", 30 September 2011, 中原农民出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113057172A (en) * | 2021-02-22 | 2021-07-02 | 安徽润农腾辉生物科技有限公司 | Ternary compound seed dressing agent containing prothioconazole and fludioxonil |
| CN115152773A (en) * | 2022-06-17 | 2022-10-11 | 安徽久易农业股份有限公司 | Composition of prothioconazole and fludioxonil for preventing and treating basal stem rot of wheat |
| CN115152773B (en) * | 2022-06-17 | 2023-12-29 | 安徽久易农业股份有限公司 | Composition of prothioconazole and fludioxonil for preventing and treating wheat stem rot |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104904723A (en) | 2015-09-16 |
| WO2015135421A1 (en) | 2015-09-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108935486A (en) | A kind of bactericidal composition | |
| CN103988845B (en) | A kind of Fungicidai mixtures | |
| CN103461362B (en) | Sterilizing active ingredient composition | |
| CN106922695A (en) | A kind of bactericidal composition | |
| CN103719125A (en) | Bactericide composition and application thereof | |
| CN105076192B (en) | A kind of Fungicidai mixtures | |
| CN108260593A (en) | A kind of bactericidal composition | |
| CN105076155B (en) | Plant disease preventing and treating composition | |
| CN107318852A (en) | A kind of bactericidal composition | |
| CN106922722A (en) | A kind of bactericidal composition | |
| CN106922697A (en) | A kind of bactericidal composition | |
| CN106922721A (en) | A kind of bactericidal composition | |
| CN106922693A (en) | A kind of bactericidal composition | |
| CN106922720A (en) | A kind of bactericidal composition | |
| CN105076178B (en) | A kind of bactericidal composition | |
| CN105076161B (en) | A kind of bactericidal composition | |
| CN105076153B (en) | A kind of plant disease control composition | |
| CN103988846B (en) | A kind of bactericidal composition | |
| CN105076148A (en) | Germ/pest killing composition | |
| CN108260597A (en) | A kind of bactericidal composition | |
| CN107593710A (en) | A kind of bactericidal composition | |
| CN105265456B (en) | A kind of bactericidal composition | |
| CN105076154B (en) | A kind of bactericidal composition | |
| CN106982842A (en) | A kind of bactericidal composition | |
| CN106922701A (en) | A kind of bactericidal composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181207 |