CN106922695A

CN106922695A - A kind of bactericidal composition

Info

Publication number: CN106922695A
Application number: CN201511008837.1A
Authority: CN
Inventors: 罗昌炎; 詹姆斯·T·布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2015-12-30
Filing date: 2015-12-30
Publication date: 2017-07-07

Abstract

The invention provides a kind of bactericidal composition, the microbicide compositions include active component Picarbutrazox and triticonazole, and the weight ratio of Picarbutrazox and triticonazole is 50:1-1:50.The present invention carries out binary built by by Picarbutrazox and triticonazole so that the present invention has obvious gain effect on preventing and treating crop by fungi, bacterial disease；In addition fungicidal spectrum is also expanded by binary built, reduces respective usage amount, reduce the risk that germ develops immunity to drugs.

Description

A kind of bactericidal composition

Technical field

The present invention relates to a kind of bactericidal composition, plant, crop or seed are more particularly, to protected from fungal disease Bactericidal composition.

Background technology

The current disease control for being agriculturally also easy to produce resistance, mixture is carried out using the different pesticide species of the mechanism of action It is optimal selection, if reasonable mixture ratio, significant synergistic effect can be produced, field control effect is substantially better than each list The effect of agent.Bactericide containing single-activity composition is usually present certain defect, continuous several times in agricultural disease preventing and treating Using not only making pathogen be also easy to produce the resistance to the action of a drug, and easily cause the pollution to food and environment, by fungicidal activity into point it Between rational mixture can overcome disadvantage mentioned above.Rational compounding makes the synergistic effect that active ingredient is produced, and can improve preventive effect, subtracts Few effective ingredient consumption, it is cost-effective, delay the drug-fast generation of pathogen, and then can mitigate or even avoid agricultural chemicals to food The pollution of thing and environment.

On pesticide activity, particularly to crop protection, one of key problem of research carried out in the technical field is Improve the performance in terms of performance, especially bioactivity and the performance for keeping within a certain period of time in terms of this activity.

Picarbutrazox, test code number NF-171；CAS：500207-04-5；Chemical formula is [6- [[[[(z)-(1- first Base -1H-TETRAZOLE -5- bases)（Phenyl）Methylene] amino] epoxide] methyl] pyridine -2- bases] t-butyl carbamate；Molecular structure Formula is：

Picarbutrazox is the oximino ether bactericide of Japanese Cao Da companies exploitation, and it has preferable to downy mildew and epidemic disease Prevention effect.Picarbutrazox is by known in CN02817805.X.

Triticonazole（Triticonazole）It is the triazole bactericidal agent developed and developed by Beyer Co., Ltd, chemical name For：(RS)-(E) -5- (4- chlorine benzal) -2,2- dimethyl -1 (1H-1,2,4- triazol-1-yls methyl) cyclopentanol；Triticonazole It is known disclosed in FR2641277.

Triticonazole is mainly used in preventing and treating cereal crop, legume, fruit diseases, has special efficacy to seed-borne disease.Sterilizing Azoles can Seed Treatment, also cauline leaf spraying.

Because the environmental requirement and economic requirement now to bactericide are constantly improve, such as to activity profile, toxicity, selection The requirement of property, rate of application, residual and favourable preparation feasibility, additionally asks due to being there may be in terms of such as drug resistance Topic, therefore, exploitation is in some aspects lasting task better than the new bactericide of existing bactericide.

Can have greater activity and the more long bactericidal composition of activity holding the invention provides a kind of.This sterilized group Compound has relatively low-dose, while the toxicity of the bactericidal composition is relatively low, fruits and vegetables and seed with fungal disease is carried out Control.

The content of the invention

It is an object of the invention to provide a kind of bactericidal composition, just reduced in the case where the reactive compound for reducing is using total amount and applied For activity profile with rate and improvement known compound Picarbutrazox and triticonazole, the present invention is reducing reactive compound Under using total amount, having to harmful fungoid improves activity（Synergistic）.

We have found that simultaneously, i.e., jointly or separately applying Picarbutrazox and triticonazole, or apply successively Picarbutrazox and triticonazole cause preferably to prevent and treat harmful fungoid than each compound is administered alone.

The invention provides a kind of bactericidal composition, said composition carries out two by by Picarbutrazox and triticonazole Unit's compounding so that the composition for obtaining has gain effect in prevention effect, and has expanded fungicidal spectrum, serves a medicine many Effect, effectively slows down or avoids germ to develop immunity to drugs.Astoundingly, the bactericidal activity of bactericidal composition of the invention It is more active plus and considerably higher than each reactive compound.In other words, there is unpredictable, necessary being collaboration effect Should, and it is not only the supplement of activity.

When reactive compound is present in bactericidal composition of the invention with specific weight ratio, cooperative effect is especially bright It is aobvious.But, the weight ratio of the reactive compound in bactericidal composition of the present invention can change within the specific limits.

A kind of bactericidal composition of the present invention is to take following technical scheme to realize：

A kind of bactericidal composition, it is characterised in that：Containing active component Picarbutrazox and triticonazole, wherein The percentage by weight of Picarbutrazox and triticonazole is 50:1-1:50, preferably 25:1-1:25,10:1-1:10, further Preferably 5:1-1:5.

The weight proportion of Picarbutrazox and triticonazole in the present invention for example can also be 50:1、45:1、40:1、 35:1、30:1、25:1、20:1、15:1、10:1、5:1、1:1、1:5、1:10、1:15、1:20、1:25、1:30、1:35、1:40、 1:45、1:50。

Picarbutrazox described in described bactericidal composition and triticonazole quality sum account for the Bactericidal composite material The 5%-90% of amount, more preferably 10%-80%, more preferably 20%-60%.

Described bactericidal composition, also comprising filler and/or surfactant.

A kind of method for preventing and treating plant pathogen, pathogenic bacteria and/or its environment, Huo Zhezhi are acted on by bactericidal composition In thing, plant parts, seed, soil, region, material or space.

It is a kind of prevent and treat plant pathogen method, by Picarbutrazox and triticonazole be administered simultaneously or apply respectively, Or sequential application.

A kind of bactericidal composition, comprising Picarbutrazox and triticonazole and filler and/or surfactant.

A kind of bactericidal composition, can be configured to any formulation for agriculturally allowing.The formulation of described bactericidal composition is Suspending agent, seed coat agent, suspension emulsion, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, Missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids.

Described bactericidal composition is used to prevent and treat Cereal, vegetables, clover, soybean, turf, timber, trees, fruit tree or garden The purposes of fungi and bacterium on skill plant.

The bactericidal composition is used to protect the purposes of plant propagation material and the plant organ for then growing.

The bactericidal composition is used to prevent and treat the purposes of fruit vegetables storing period disease.

The bactericidal composition be applied to needed for preventing and treating place preventing and treating soil cause a disease or saprophytic fungi and bacterium use On the way.

A kind of plant pathogenic fungi of control plant, plant part, plant propagation material and the plant organ for then growing Method, the method include by the amount of application of described bactericidal composition effective and basic plant-less toxicity with agronomy with seed at The methods such as reason, foliage applying, stem are applied, are impregnated with, instiling, pouring into a mould, spraying, spraying, dusting, distribution or smoke be administered to plant, Plant part, plant propagation material or plant are being grown or needed in the soil or cultivation medium for growing wherein.

Described bactericidal composition is in various crop plants such as banana, cotton, vegetable variety（Such as cucumber, beans, tomato And cucurbitaceous plant）, it is barley, dogstail, oat, coffee, potato, corn, fruit variety, rice, rye, soybean, grapevine, small A large amount of fungies are prevented and treated in wheat, ornamental plant, sugarcane and a large amount of seeds to be even more important.

Bactericidal composition of the invention has very strong activity to various phytopathogens, and can be to being drawn by phytopathogen The prevention and treatment of the plant disease for rising play very strong preventive effect.Bactericidal composition of the invention to such as Plasmodiophoromycetes, Oomycete, Chytridiomycetes, connect there is pole with the wide scope plant pathogenic fungi such as Gammaproteobacteria, Ascomycetes, Basidiomycetes, deuteromycetes Good activity.

Bactericidal composition of the invention also has extraordinary bactericidal performance, and it is thin to can be used for preventing and treating plant pathogenic Bacterium.Such as pseudomonadaceae, Rhizobiaceae, enterobacteriaceae, bar Cordycepps and Streptomycetaceae.

Bactericidal composition of the invention can be used as blade face fungicide in crop protection, can act also as fungicide use In seed dressing and as soil fungicide.

Bactericidal composite of the invention also can be used to prevent and treat the disease of fruit vegetables storing period.

Bactericidal composition of the invention is particularly suitable for the following phytopathogen of preventing and treating：

As oomycetes subclass, can be exemplified as, such as various crops such as the corruption of black leg of beet bacterium (Pythium ultium) Mould category bacterium；Such as potato blight bacterium (PhytophthoraiNfestaNs), botrytis cinerea (Phytophthora Capsici Phytophthora category bacterium)；Such as bacterium of downy mildew of cucumber (PseudoperoNospora cubeNsis), Humulus The Pseudoperonospora bacterium of Pseudoperonospora cubensis (PseudoperoNospora humuli)；Such as Plasmopara viticola (Plasmopara Viticola Plasmopara bacterium)；Such as Pseudoperonospora cubensis (PeroNospora brassicae), the green onion downy mildew of brassicaceous vegetable Germ (PeroNospora destructor), Peronospora bacterium of downy mildew of spinach bacterium (PeroNospora spiNaciae) etc..

As Ascomycetes, can be exemplified as, for example, such as the powdery mildew of powdery mildew of cereals bacterium (ErysiphegramiNis) Category bacterium；Such as the Sphaerotheca bacterium of greengrocery powdery mildew (Sphaerotheca fuligiNea)；Such as apple black star bacteria The Venturia bacterium of (VeNturia iNaequalis), pear cucumerinum (VeNturia Nashicola)；Such as net blotch of barley The PyreNophora category bacterium of bacterium (PyreNophorateres)；Such as wheat class spot disease (Cochliobolus sativus) Cochliobolus belongs to bacterium；Such as Sclerotinia bacterium of greengrocery hyphal cluster germ (SclerotiNia sclerotiorum).

As Basidiomycetes, can be exemplified as, for example, such as double spores of wheat leaf rust germ (PucciNiarecoNdita) Rust bacterium；Such as the Tilletia bacterium of bunt of wheat bacterium (Tilletia caries)；Such as loose smut of barley bacterium The Ustilago bacterium of (Ustilago Nuda) etc.

As Deuteromycotina, can be exemplified as, for example, such as the Phoma of lucid asparagus Phoma asparagi Sacc (Phomaasparagi) Bacterium；Such as the Septoria bacterium of wheat class crust rot bacterium (Septoria Nodorum)；Such as Colletotrichumlagenarium The Colletotrichum bacterium of (Colletotrichum lageNarium)；Such as wilt (Pyricularia oryzae) Pyricularia belongs to bacterium；Such as the Botrytis bacterium of greengrocery ash arrhizus bacteria (Botrytis ciNerea)；Such as apple spot fallen leaves Germ (AlterNaria mali), the Alternaria bacterium of tomato early blight bacterium (AlterNaria solaNi)；Such as beet Cercospora The Cercospora bacterium of germ (Cercospora beticola)；Such as peach black star germ (Cladosporium carpophilum) Blastocladia bacterium；Such as Rhizoctonia bacterium of Oryza sheath blight fungus (RhizoctoNia solaNi).

Suitable crop mainly includes field crop, such as corn, soybean, cotton, rape oilseeds, such as southern general rape (Brassica napus) (such as rape (canola)), overgrown with weeds green grass or young crops (Brassica rapa), leaf mustard (B.juncea) (such as mustard Sub (mustard)) and brassicacarinata (Brassica carinata), rice, wheat, beet, sugarcane, oat, brown wheat, greatly Wheat, broomcorn millet, triticale, flax, grapevine and the other fruit of various plants or vegetable crop, such as rose family (Rosaceaesp.) (for example, pip fruit, such as apple and pears, also drupe, such as apricot, cherry, almond and peach, berry such as strawberry), tea sugarcane Scarabaeidae (Ribesioidae sp.), Juglandaceae (Juglandaceae sp.), Betulaceae (Betulaceae sp.), Anacardiaceae (Anacardiaceae sp.), Fagaceae (Fagaceae sp.), Moraceae (Moraceae sp.), Oleaceae (Oleaceae Sp.), Actinidiaceae (Actinidaceae sp.), Lauraceae (Lauraceae sp.), Musaceae (Musaceae sp.) be (for example Banana tree and powder bajiao banana (plantings)), Rubiaceae (Rubiaceae sp.) (such as coffee), Theaceae (Theaceae Sp.), Sterculiaceae (Sterculiceaesp.), Rutaceae (Rutaceae sp.) (such as lemon, orange and grape fruit)；Solanaceae (Solanaceaesp.) (for example, tomato, potato, pepper, eggplant), Liliaceae (Liliaceae sp.), composite family (Compositiae sp.) (such as lettuce, globe artichoke and witloof-include root witloof (root chicory), hare's-lettuce (endive) Or common witloof (common chicory)), Umbelliferae (Umbelliferae sp.) (such as carrot, parsley, celery and block Rooted celery), Curcurbitaceae (Cucurbitaceae sp.) (for example cucumber-include pickled cucumber (pickling cucumber), south Melon, watermelon, cucurbit and muskmelon), green onion section (Alliaceae sp.) (such as onion and leek), Cruciferae (Cruciferae Sp.) (for example white cabbage, red cabbage, broccoli, cauliflower, brussels sprout, green vegetables, kohlrabi, radish, horseradish, solely Row dish, Chinese cabbage), pulse family (Leguminosaesp.) (such as peanut, pea and French beans-such as climning bean and broad bean), Chenopodiaceae (Chenopodiaceae sp.) (such as fodder beet, spinach sweet (spinach beet), spinach, beet root), Malvaceae (Malvaceae) (such as gumbo), Asparagaceae (Asparagaceae) (such as lucid asparagus)；Garden crop and forest crop； Ornamental plant；And the homologue of the genetic modification of these crops.

The preferably for example following disease of Bactericidal composite of the invention etc. is prevented and kill off：

The brown spot (Cercospora beticola) of " beet ", black root（Aphanomyces cochlloides）, root-rot Disease（Thanatephorus cucumeris）, leaf rot（Thanatephorus cucumeris）；

The brown spot of " peanut "（Mycosphaerella arachidis）, black spot（Mycosphaerella berkeleyi）；

The powdery mildew of " cucumber "（Sphaerotheca fuliginea）, downy mildew（Pseudoperonospora cubensis）, blight dis-ease（Mycosphaerella melonis）, dead arm（Fusarium oxysporum）, sclerotiniose （Sclerotinia sclerotiorum）, gray mold（Botrytis cinerea）, anthracnose（Colletotrichum orbiculare）, scab（Cladosporium cucumerinum）, brown spot（Corynespora cassicola）, seedling Damping-off（Pythium debaryanam, Rhizoctonia solani Kuhn）, bacterial leaf spot（Pseudomonas syringae pv.Lecrymans）；

The gray mold of " tomato "（Botrytis cinerea）, leaf mold（Cladosporium fulvum）, late blight （Phytophthora infestans）；

The gray mold of " eggplant "（Botrytis cinerea）, bacterial blight（Corynespora melongenae）, powdery mildew （Erysiphe cichoracearum）, suede bacterial plaque disease（Mycovellosiella nattrassii）；

The gray mold of " strawberry "（Botrytis cinerea）, powdery mildew（Sohaerotheca humuli）, anthracnose （Colletotrichum acutatum、Colletotrichum fragariae）, epidemic disease（Phytophthora cactorum）；

The neck rot of " onion "（Botrytis allii）, gray mold（Botrytis cinerea）, hickie leaf blight （Botrytis squamosa）, downy mildew（Peronospora destructor）；

The root knot of " cabbage "（Plasmodiophora brassicae）, soft rot（Erwinia carotovora）, frost Mildew（Peronospora parasitica）；

The sclerotiniose of " French beans "（Sclerotinia sclerotiorum）, gray mold（Botrytis cinerea）；

The powdery mildew of " apple "（Podosphaera leucotricha）, scab（Venturia inaequalis）, spend corruption Disease（Monilinia mali）, diplostomiasis（Mycosphaerella pomi）, rot（Valsa mali）, spot defoliation （Alternaria mali）, rust（Gymnosporangium yamadae）, ring spot（Botryosphaeria berengeriana）, anthracnose（Glomerella cingulata、Colletotrichum acutatum）, brown spot （Diplocarpon mali）, fly pinta（Zygophiala jamaicensis）, sooty mould（Gloeodes pomigena）；

The powdery mildew of " persimmon "（Phyllactinia kakicola）, anthracnose（Gloeosporium kaki）, angle spot fallen leaves Disease（Cercospora kaki）；

The brown rot of " peach "（Monilinia fructicola）, scab（Cladosporium carpophilum）, intend stem Point mildew（Phomopsis sp.）；

The brown rot of " cherry "（Monilinia fructicola）；

The gray mold of " grape "（Botrytis cinerea）, powdery mildew（Uncinula necator）, late blight （Glomerella cingulata、Colletotrichum acutatum）, downy mildew（Plasmopara viticola）, it is black Acne disease（Elsinoe ampelina）, brown spot（Pseudocercospora vitis）, black rot（Guignardia bidwellii）；

The scab of " pears "（Venturia nashicola）, rust（Gymnosporangium asiaticum）, black spot （Alternaria kikuchiana）, ring spot（Botryosphaeria berengeriana）, powdery mildew （Phyllactinia mali）；

The zonate spot of " tea "（Pestalotia theae）, anthracnose（Colletotrichum theae-sinensis）；

The shot hole of " citrus "（Elsinoe fawcetti）, penicilliosis（Penicillium italicum）, green mould （Penicillium digitatum）, gray mold（Botrytis cinerea）, diplostomiasis（Diaporthe citri）, ulcer Disease（Xanthomonas campestris pv.Citri）；

The powdery mildew of " wheat "（Erysiphe graminis f.sp.tritici）, head blight（Gibberella zeae）, leaf Rust（Puccinia recondita）, brown snow mold（Pythium iwayamai）, snow red rot （Monographella nivalis）, wheat basal stem rot（Pseudocercosporella herpotrichoides）, leaf it is withered Disease（Septoria tritici）, glume blight（Leptosphaeria nodorum）, the rotten sclerotium oryzicola of snow（Typhula incarnata）, snow big grain sclerotinia rot（Myriosclerotinia borealis）, damping-off（Gaeumanomyces graminis）；

The stripe disease of " barley "（Pyrenophora graminea）, clouding disease（Rhynchosporium secalis）, dissipate it is black Fringe disease（Ustilago tritici、U.nuda）；

The rice blast of " rice "（Pyricularia oryzae）, banded sclerotial blight（Rhizoctonia solani）, bakanae disease （Gibberella fujikuroi）, flax leaf spot（Cochliobolus niyabeanus）, seedling blight（Pythium graminicola）, bacterial leaf-blight（Xanthomonas oryzae）, bacillary seedling blight（Burkholderia plantarii）, brown streak（Acidovorax avenae）, the withered phytosis of paddy（Burkholderia glumae）

The sclerotiniose of " tobacco "（Sclerotinia sclerotiorum）, powdery mildew（Erysiphe cichoracearum）；

The gray mold of " tulip "（Botrytis cinerea）；

The snow big grain sclerotinia rot of " Western Korea lawn grass "（Sclerotinia borealis）, samping off（Pythium aphanidermatum）；

The powdery mildew of " orchard grass "（Erysiphe graminis）；

The purple spot of " soybean "（Cercospora kikuchii）, downy mildew（Peronospora Manshurica）, cane blight （Phytophthora sojae）；

Potato, tomato late blight（Phytophthora infestans）.

Bactericidal composition of the invention can be good preventing and treating cause pathogen and the medicament drug-fast bacteria of following disease：Paddy rice Seedbed damping-off, bakanae disease；Disease in cotton seedling stage such as damping-off, anthracnose, samping off, red rot；Vegetable nursery bed disease is for example sudden Sick anthracnose, damping-off, the phytophthora root rot of tomato, gray mold, leaf rust, spot disease, anthracnose and early phytophthora root rot；The frost of cucumber Mildew, powdery mildew, gray mold, anthracnose, withered rattan disease, black spot and brown spot；The anthracnose and gray mold of strawberry；Pumpkin it is white Powder disease, anthracnose and withered rattan disease；The hickie leaf blight of onion, black spot, gray mold and Mild Powder；The powdery mildew of eggplant, grey mold Disease and bacterial blight；The black spot of shallot；The Leucoplakia and black spot of Chinese cabbage；The sclerotiniose and gray mold of green pepper；Cucumber it is white Powder disease, anthracnose and withered rattan disease；The powdery mildew of muskmelon, anthracnose and withered rattan disease；The gray mold of lettuce；The black spot and ash of red bayberry Mildew；The powdery mildew of persimmon, anthracnose, gray mold and defoliation；The gray mold of oranges and tangerines；The scab of pears, black spot and early epidemic Mildew；The gray mold of grape, anthracnose, brown spot, black rattan are sick, withered rattan is sick, viscous rot and the swollen disease of branch；The scab of peach, ash Pinta, anthracnose；The scab of apple, zonate spot, early phytophthora root rot, brown spot, coal point disease, coal spot disease, diplostomiasis and brown rot； The stem wilt of mung bean, anthracnose, gray mold and sclerotiniose；The anthracnose of Kidney bean, gray mold and sclerotiniose；The ring spot of broad bean； Purple spot of soybean；The powdery mildew of tobacco, the brown spot of beet and spot disease；The anthracnose of tea, zonate spot and the withered disease of sprouting and Gray mold；Spend the plant diseases such as scab, anthracnose, gray mold and the powdery mildew of class.Bactericidal composition of the invention is particularly Preventing and treating tomato that can be good, cucumber, eggplant, strawberry, grape, the epidemic disease of peach and oranges and tangerines, powdery mildew and the gray mold cause of disease Phytopathogen and medicament drug-fast bacteria.

Bactericidal composition of the invention is especially suitable for preventing and treating apple, banana, grape, oranges and tangerines, mango, paddy rice, wheat, jade Downy mildew on rice, potato, soybean, tomato, capsicum, cucumber, eggplant, grape, early blight, late blight, balck shank, scab, Leaf spot, anthracnose, powdery mildew, wilt disease, damping-off, samping off.

The crop of bactericidal composition treatment of the invention is such as but not limited to cereal, vegetables, clover, soybean, turf, wood Material, trees, fruit tree or gardening plant.

Fungicidai mixtures of the invention can also be used to prevent and treat fruit vegetables storing period disease.The fruit for for example being caused by following pathogen It is real to rot：

Aspergillus bacterium, such as aspergillus flavus；

Botrytis（Botrytis）Strain, such as Botrytis cinerea（Botrytis cinerea）;

Penicillium（Penicillium）Strain, such as penicillium expansum（Penicillium expansum）With P.purpurogenum;

Sclerotinia（Sclerotinia）Strain, such as sclerotinite（ Sclerotinia sclerotiorum）;

Verticillium（Verticilium）Strain, such as black and white wheel branch spore（Verticilium alboatrum）.

Bactericidal composition of the invention be also particularly well suited for preventing and treating fruit vegetables storing period disease blossom-end rot, green mould, penicilliosis, Anthracnose.

Fungicidai mixtures of the invention can be additionally used in preventing and treating kind and pass and soil-borne disease.The kind for for example being caused by following pathogen Pass and native rotten and wilting disease and the seedling disease for passing：

Alternaria category kind, such as rape give birth to rod method (Alternaria brassicicola);

Aphanomyces (Aphanomyce) category kind, such as mould (Aphanomyces of Kidney bean silk capsule

euteiches) ;

Ascochyta (Ascochyta) category kind, such as Ascochyta lentis;

Aspergillus, such as aspergillus flavus;

Cladosporium category kind, such as draft branch spore (Cladosporium herbarum);

Cochliobolus category category kind, such as standing grain cochliobolus;

(conidial form:Drechslera, Syn:The compacted spore bacterium of length);

Colletotrichum category kind, such as hair core anthrax-bacilus (Colletotrichum coccodes);

Fusarium category kind, such as yellow fusarium;

Gibberella category kind, such as beautiful another name for Sichuan Province multitude is red mould;

Shell ball spore category (Macrophomina) category kind, such as Kidney bean shell ball spore (Macrophomina

phaseolina) ;

Small setting-out shell belongs to strain, for example, avenge rotten small setting-out shell;

Penicillium category kind, such as penicillium expansum;

Phaeosphaeria strains, such as Phaeosphaeria nodorum;

Phoma (Phoma) category kind, such as black embryo Phoma sp (Phoma lingam);

Phomopsis (Phomopsis), such as soybean Phomopsis (Phomopsis sojae);

Phytophthora category kind, such as Phytophthora cactorum (Phytophthora cactorum);

Nuclear cavity Pseudomonas category kind, such as wheat class nuclear cavity bacteria (Pyrenophora graminea);

Pyricularia Sacc. (Pyricularia) category kind, such as Magnaporthe grisea (Pyricularia oryzae);

Pythium kind, such as Pythium ultimum;

Rhizoctonia category kind, such as Rhizoctonia solani Kuhn;

Rhizopus (Rhizopus) category kind, such as Rhizopus oryzae (Rhizopus oryzae);

Bulbil category (Sclerotium) category kind, such as Sclerotium rolfsii (Sclerotium rolfsii);

Core coral Pseudomonas (Typhula) category kind, such as meat spore core coral bacterium (Typhula incarnata);

Verticillium (Verticillium) category kind, such as verticillium dahliae (Verticillium dahliae).

Bactericidal composition of the invention also has excellent bactericidal effect to the bacterium of existing bactericide display tolerance.For example Ash arrhizus bacteria（Botrytis cinerea）, sugar beet leaf spot bacteria（Cercospora beticola）, apple black star bacteria （Venturia inaequalis）, pear cucumerinum（Venturia nashicola）Deng.

A kind of plant pathogenic fungi of control plant, plant part, plant propagation material and the plant organ for then growing Method, including by the amount of application of the bactericidal composition of the invention effective and basic plant-less toxicity with agronomy with seed at The methods such as reason, foliage applying, stem are applied, are impregnated with, instiling, pouring into a mould, spraying, spraying, dusting, distribution or smoke be administered to plant, Plant part, plant propagation material or plant are being grown or needed in the soil or cultivation medium for growing wherein.

Bactericidal composition of the invention can be spread by Seed Treatment, cauline leaf, soil application or it is used for ponds etc. and The prevention and treatment of the various diseases for being produced when cultivation includes the agriculture garden crop of flowers, lawn, herbage.

The present invention provides a kind of method for preventing and treating plant pathogen, and bactericidal composition is acted on into pathogenic bacteria and/or its ring In border, or plant, plant parts, plant propagation material, soil, region, material or space.

Bactericidal composition of the invention can be used for the plant device protected plant part, plant propagation material and then grow Official.

Bactericidal composition of the invention, can process all plants and plant part." plant " refers to all plants and plant Population, such as preferably with undesirable wild plant, cultivated plant and plant variety (regardless of whether receiving plant variety or plant Cultivate the protection of people's right).Cultivated plant and plant variety can be the plants obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique method, such as using dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Above and below the ground part and organ, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.Crop and nourish and generate and case of propagation material, for example, transplant, bulb, rhizome, running roots Plant part is fallen within seed.

Term " plant propagation material " is interpreted as referring to all plant parts for having a fertility, such as seed, and it can be used In breeding the latter, and vegetable matter such as cuttage bar or stem tuber（Such as potato）.Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.Treat The germination plant and effective plant suppressed after being germinateed from soil or after emerging.Young plant can before transplantation by being impregnated into Row whole or Local treatment are protected.

Currently preferred propagating materials is seed.The method of Seed Treatment, for example, can there are, diluent liquid or solid The medicament of shape or seed is directly immersed in liquid condition solution the method for making medicament be impregnated with seed without dilution, by solid Medicament or liquid preparation are mixed with seed, be coated treatment make the surface of the seed adhere to medicament method, plantation Simultaneously near seed sprinkling etc. method.

Plant part and the plant organ for then growing are by any of the plant propagation material such as plant of seed generation Part.Plant part, plant organ and plant can also be benefited from and be applied to plant propagation material institute by by bactericidal composition The pathogenic damage protection of acquisition.The plant organ grown behind certain plants part and some places can also regard plant propagation as Material, its own can be applied with bactericidal composition（Or treatment）；So as to by the plant part through processing and the plant through processing Plant, other plant parts and the other plant organs that organ is produced can also be benefited from and applied by by bactericidal composition With.

Bactericidal composition of the invention can also be used to prevent or control soil or cultivate various in medium causing a disease or saprophytic Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria（Alternaria spp.）, Ascochyta (Ascochyta spp.), Botrytis cinerea（Botrytis cinerea, Cercospora（Cercospora spp., ergot （Claviceps purpurea）, standing grain cochliobolus（Cochliobolus sativus）, Colletotrichum（colletotrichum Spp., Epicoccum（Epicoccum spp., F.graminearum schw（Fusarium graminearum）, rice evil seedling chain spore （Fusarium moniliforme）, fusarium oxysporum（Fusarium oxysporum, fusarium moniliforme（Fusarium proliferatum）, fusariun solani (Fusarium solani) ties up glue chain spore (Fusarium subglitinans), long compacted Spore belongs to (Helminthosporium spp), rotten microtorr bacterium (Microdochium nivale) of snow, Penicillium (Pencillium Spp), Phoma (Phoma spp.), wheat class nuclear cavity bacteria (Pyrenophora graminea), rice blast Pyricularia Sacc. (Pyricularia oryzae), Rhizoctonia solani Kuhn (Rhizoctonia solani), Rhizoctonia cerealis (Rhizoctonia Cerealis), Sclerotinia (Sclerotinia spp.), Septoria (Septoria spp.), silk axle smut (Sphacelotheca reilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula Incarnate), hidden bar smut (Urocystis occulta), Ustilago (Ustilago spp.) or Verticillium (Verticillium spp.)。

Soil germ has rhizoctonia solani, sickle-like bacteria, phytophthora, bacterium of dampinging off, root rot, pythium spp, botrytis cinerea, soft-rot fungi Deng.In general, soil germ can produce a large amount of thalline, as long as condition is favourable to pathogen growth development, host is sense Disease, germ just with amount reproduction and can infect host, and in the presence of susceptible host, these germs can just enter lasting The phase of causing a disease, the amount reproduction diffusion with the continuous cropping of crop, but nutrient is depleted or edaphic condition such as temperature, humidity etc. afterwards When unfavorable to germ, germ can enter rest period again.When susceptible host does not exist, soil-borne disease bacterium can also survive in soil Get off, in addition to soil germ has extensive host range, moreover it is possible to survived on the root surface of non-host or the broken branches and fallen leaves, with it It is undivided with saprophytic competitiveness.But different germs are discrepant, as sickle-like bacteria almost can be unlimited in soil Phase survives.

Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, for example：Soil, water etc., Specific raw material can using such as sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Wood chip, bark etc..

To the method in soil using medicament, for example, liquid preparation is diluted in water or do not diluted and be directly applied to plant The medium method in the root of body or the rice seedling bed of seedling, the side in the rice seedling bed of the root of plant or seedling is disseminated to by granule Method has the method that pulvis, water dispersible granules etc. are sprayed in soil and are integrally mixed with soil prior to seeding, and sowing is preceding or plants In being sprayed on implantation hole, seed furrow after pulvis, water-dispersible grain dilution agent before kind plant, in method sowed etc..

Another object of the present invention, there is provided a kind of control plant, plant part, plant propagation material and then grow The method of the plant pathogenic fungi of plant organ, the method include by bactericidal composition of the invention with agronomy it is effective and substantially without Phytotoxic amount of application is applied with Seed Treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution Or the method such as smoke is administered to plant, plant part, plant propagation material or plant and is growing or needing what is grown wherein In soil or cultivation medium.

Bactericidal composition of the invention can be applied by different processing methods, and these methods are for example：

- liquid comprising the bactericidal composition is sprayed onto the aerial part of the plant；

- dusting, mixes particle or powder in soil, in surrounding plants sprinkling, and in tree injection or the situation of smearing Under；

- seed of plant is coated or film coated.

- for postharvest fruit and vegetable anti-corrosive fresh-keeping when, 200-2000 times of liquid of usual dilute with water, leaching fruit after leach.

The present invention provides a kind of method for preventing and treating plant pathogen, can treat, prevent or method of eradication.

Bactericidal composition of the invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid The dosage forms such as agent, granule, seed coat agent are used, its amount of application, mixing ratio, meteorological condition according to active ingredient, medicament Form, Dressing date, application process, using place, prevent and kill off the different and variant of object noxious livings, objective crop etc..

Treatment in accordance with the present invention is there may be super plus (" collaboration ") effect.For example, sterilized group used according to the present invention The rate of application of compound and/or widen its field of activity and/or increase its activity, it is possible to obtain following effect：More preferable plant Growth, the tolerance to high temperature or low temperature increases, and the tolerance to arid or water or soil salt content increases, and performance of blooming is carried It is high, it is easier to harvest, the maturation of quickening, yield rate higher, bigger fruit, plant height higher, the color of leaf is more It is green, to bloom earlier, the quality or nutritive value of the product of results are higher, and sugared concentration is higher in fruit, the storage of the product of results More preferably, these benefits have exceeded the effect actually estimated for stability and/or processability.

Processing method of the invention can be additionally used in process propagating materials such as stem tuber or rhizome, and can be used for treatment seed, Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for processing root.The present invention Processing method can also be used for processing the aerial part of plant such as about the doing of plant, stem or stalk, leaf, flower and fruit.

- generally for leaf portion treatment：0.1-10000g/ha, preferably 10-1000 g/ha, more preferably 50-500 g/ha；It is right For dipping or administration of instiling, the dosage can also even be reduced, particularly when using inert base such as asbestos or pearl During rock；

- for Seed Treatment：2-5000g/100kg seeds, preferably 3-1000g/100kg seeds；

- for soil or treatment used for ponds：0.1-10000g/ha, preferably 1-1000g/ha；

- fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, leached after leaching fruit.

Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to actual feelings during actual administration Condition and needs, the seriousness especially in accordance with the property and germ of pending plant or crop adjust rate of application.

Picarbutrazox of the invention is combined with triticonazole/co-administered.Including separating, sequentially or simultaneously applying Picarbutrazox and triticonazole.Preferably, the Picarbutrazox is combined as comprising Picarbutrazox with triticonazole With the form of the composition of triticonazole.

Composition of the invention can be based on dosage form, i.e., each material is blended in composition, composition into Point can also unit dose form provide, use it is preceding mix in bucket or tank, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.

As a further improvement on the present invention, bactericidal composition of the invention can be configured to any agent for agriculturally allowing Type.

As a further improvement on the present invention, the formulation of bactericidal composition of the invention is suspending agent, seed coat agent, wettable It is pulvis, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, super Low capacity liquor, suspension emulsion.

In bactericidal composition of the present invention, lived comprising Picarbutrazox and triticonazole, filler and/or surface Property agent.

The content of bactericidal composition of the present invention, wherein Picarbutrazox and triticonazole accounts for bactericidal composition 5%-90%。

The content of described bactericidal composition, wherein Picarbutrazox and triticonazole accounts for the 10%- of bactericidal composition 80%。

The content of described bactericidal composition, wherein Picarbutrazox and triticonazole accounts for the 20%- of bactericidal composition 60%。

According to the present invention, term " filler " refers to can be combined with reactive compound or combines to make it easier for being administered to Object（Such as plant, crop or careless class）Natural or synthetic organic or inorganic compound.Therefore, the filler is preferably It is inert, at least should be agricultural acceptable.The filler can be solid or liquid.

The nonactive medium that can be used in the present invention both can be that solid can also be liquid, can be as solid matchmaker What dielectric material was used has for example：Phyteral powdery type（Such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut The particle of shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem, extracts residue after plant essence etc.）, paper , sawdust, crush synthesized polymer body, the clay class of synthetic resin etc.（Such as kaolin, bentonite, acid china clay etc.）, talcum powder Class.Silica（Such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates）, activated carbon, natural mineral matter class（Float stone, Lv Po Thread stone and zeolite etc.）, fire diatomite, sand, plastics medium etc.（Such as polyethylene, polypropylene, polyvinylidene chloride etc.）, chlorination The chemical fertilizer of inorganic mineral powder, ammonium sulfate, ammonium phosphate, urea, the ammonium chloride of potassium, calcium carbonate, calcium phosphate etc. etc., soil Fertilizer, these materials can be used alone or two or more is mixed.

Can be selected in llowing group of materials as liquid media materials'use, such as water, alcohol type（Such as first Alcohol, ethanol, isopropanol, butanol, ethylene glycol etc.）, ketone（Such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng）, ethers（Such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.）, aliphatic hydrocarbon class（Such as kerosene, Mineral oil etc.）, aromatic hydrocarbons class（Such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro fat Fat hydrocarbon, chlorobenzene, etc.）, halogenated hydrocarbon class, amide-type, sulfone class, dimethyl sulfoxide (DMSO), mineral and vegetable oil, animal oil etc..

To emulsify active ingredient compound, dispersion and/or soak, it is possible to use surfactant for example can be with Enumerate AEO, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the fatty acid ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total to Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as thing, alkyltaurate, protein hydrolysate.Suitable oligosaccharide thing or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or its with for example（It is polynary）Alcohol or（It is polynary）The combination of amine.

To make active ingredient compound dispersion stability, attachment and/or combining, such as xanthans, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid（Such as cephalin and lecithin）With And the adjuvant such as synthetic phospholipid, bentonite, sodium lignin sulfonate.

The wherein optional spent glycol of antifreezing agent, propane diols, glycerine, sorbierite.As the deflocculant of suspension product The adjuvant of such as naphthalene sulfonic acid polymer, polymeric phosphate etc. can be used.

Organic silicon defoamer can be used as defoamer.

The colouring agent that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff：Alizarin dyes, azo dyes and metallized phthalocyanine dye；And trace element, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.

Optionally, can also include other annexing ingredients, for example protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.

The reactive compound can be mixed and prepared by the preparation of the invention by known way with conventional additives. The conventional additives such as conventional extender and solvent or diluent, emulsifying agent, dispersant, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, drier and colouring agent, stabilizer, pigment, defoamer, preservative, increasing can also be included Thick dose, water and other processing aids.

Bactericidal composition of the invention not only includes can be by suitable equipment as spraying or dusting equipment are applied to immediately Pending object, and the concentration commercial composition for needing to be diluted is additionally included in before being applied to object.

It is of the invention to be applied with other active ingredient combinations containing Picarbutrazox and triticonazole, for example for Expand activity profile or prevent from forming resistance.Described other active components such as fungicide, bactericide, attractant, kill insect Agent, acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..

Reactive compound Picarbutrazox and triticonazole can be administered simultaneously, or apply respectively, or sequential application, separate Using when order on prevent and treat result generally without influence.

In the case where reactive compound is reduced using total amount, have to harmful fungoid improves activity to bactericidal composition of the invention （Synergistic）.And bactericidal composition of the invention shows that the bacterium of tolerance also has excellent killing to existing bactericide Bacterium effect.

A kind of bactericidal composition of the present invention, said composition carries out binary built by by Picarbutrazox and triticonazole, So that the composition for obtaining has gain effect in prevention effect, and fungicidal spectrum is expanded, served the multiplex work of a medicine With effectively slowing down or avoid germ to develop immunity to drugs.The bactericidal activity of bactericidal composition of the invention is than each reactive compound It is active plus and it is considerably higher, there is unpredictable, necessary being cooperative effect, and be not only the supplement of activity.

The present invention is that a kind of can have greater activity and activity keeps more long bactericidal composition.The bactericidal composition With relatively low-dose, while the toxicity of the bactericidal composition is relatively low, fruits and vegetables and seed with fungal disease are controlled.

Specific embodiment

The invention will be further elaborated to first pass through specific example of formulations below.

Example of formulations

Embodiment 1：30% Picarbutrazox triticonazole wettable powders

Picarbutrazox20%, triticonazole 10%, lignosulfonates 6%, spaonin powder 4%, attapulgite adds to 100%, will Aforementioned formula material mixes, the uniform stirring in stirred tank, is remixed after airslide disintegrating mill uniformly, that is, be obtained 30% Picarbutrazox triticonazole wettable powders.

Embodiment 2：60% Picarbutrazox triticonazole wettable powders:

Picarbutrazox 50%, triticonazole 10%, naphthalene sulfonic acid-formaldehyde condensation product 8%, tea seed cake 5%, kaolin adds to 100%, will be preceding Formulation material mixing is stated, the uniform stirring in stirred tank is being well mixed after airslide disintegrating mill, is obtained 60% Picarbutrazox triticonazole wettable powders.

Embodiment 3：30% Picarbutrazox triticonazole wettable powders

Picarbutrazox 10%, triticonazole 20%, AEO 7%, wetting and penetrating agent F 5%, diatomite is added to 100%, aforementioned formula material is mixed, the uniform stirring in stirred tank, it is being well mixed after airslide disintegrating mill, it is obtained 30% Picarbutrazox triticonazole wettable powders.

Embodiment 4：40% Picarbutrazox triticonazole water dispersible granules

Picarbutrazox30%, triticonazole 10%, alkyl benzene sulfonate calcium salt 8%, Nekal BX 6%, ammonium sulfate 2%, bentonite adds To 100%, aforementioned formula material is uniformly mixed, with micro jet, through mediating, being subsequently adding fluidized-bed granulation dryer In granulated, dry, sieve after be mixed to prepare 40% Picarbutrazox triticonazole water dispersible granules through sampling analysis.

Embodiment 5：60% Picarbutrazox triticonazole water dispersible granules

Picarbutrazox40%, triticonazole 20%, aliphatic acid polyethenoxy ether 7%, lauryl sodium sulfate 4%, aluminium chloride 2.8%, kaolin adds to 100%, and aforementioned formula material is uniformly mixed, with micro jet, through mediating, being subsequently adding fluidisation Bed granulating and drying machine in granulated, dry, sieve after be mixed to prepare 60% Picarbutrazox triticonazoles through sampling analysis Water dispersible granules.

Embodiment 6：90% Picarbutrazox triticonazole water dispersible granules

Picarbutrazox60%, triticonazole 30%, octylphenol polyethylene oxyethylene group ether sulfate 1%, neopelex 1%, Sodium carbonate 1%, white carbon adds to 100%, and aforementioned formula material is uniformly mixed, with micro jet, through mediating, being subsequently adding Granulated in fluidized-bed granulation dryer, dried, sieve after through sampling analysis i.e. be obtained 90% Picarbutrazox sterilize Azoles water dispersible granules.

Embodiment 7：30% Picarbutrazox sterilizing azole suspending agents

Picarbutrazox20%, triticonazole 10%, polycarboxylate 6%, silicone oil 0.3%, xanthans 0.9%, diethylene glycol (DEG) 2.5%, go from Sub- water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to active ingredient is crossed, and mixes by high speed shear Uniformly, active ingredient is added, ball milling 2 ~ 3 hours in ball mill make particle diameter all below 5 μm, that is, is obtained 30% Picarbutrazox sterilizing azole suspending agents.

Embodiment 8：20% Picarbutrazox sterilizing azole suspending agents

Picarbutrazox10%, triticonazole 10%, naphthalene sulfonic acid-formaldehyde condensation product 6%, hydroxyethyl cellulose 0.7%, silicone chemical combination Thing 0.1%, polyethylene glycol 2%, deionized water adds to 100%, remaining group after thickener and antifreezing agent are mixed in addition to active ingredient Point, it is well mixed by high speed shear, add active ingredient, ball milling 2 ~ 3 hours in ball mill, make particle diameter all 5 μm with Under, that is, 20% Picarbutrazox sterilizing azole suspending agents are obtained.

Embodiment 9：40% Picarbutrazox sterilizing azole suspending agents

Picarbutrazox25%, triticonazole 15%, lignosulfonates 8%, Magnesiumaluminumsilicate 1%, silicone 0.2%, glycerine 2.8%, deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to active ingredient, is cut by high speed Cut well mixed, add active ingredient, ball milling 2 ~ 3 hours in ball mill make particle diameter all below 5 μm, i.e., prepared 40% Picarbutrazox sterilizing azole suspending agents.

Embodiment 10：20% Picarbutrazox triticonazole suspension emulsions

Picarbutrazox 10%, triticonazole 10%, SOLVESSO^TM100 10%, alkyl benzene sulfonate calcium salt 7%, methylcellulose 1.2%, propane diols 2.4%, C_8~10Aliphatic alcohols 0.4%, Tween 80 7%, water adds to 100%；Add in the continuous phase containing triticonazole Enter the suspended phase of finely ground Picarbutrazox, be mixed to prepare 20% Picarbutrazox triticonazole suspension emulsions.

Embodiment 11：20% Picarbutrazox triticonazole suspension emulsions

Picarbutrazox15%, triticonazole 5%, lignosulfonates 6%, xanthans 0.8%, ethylene glycol 2.5%, SOLVESSO^TM100 15%, silicone oil 0.2%, 600# phosphates 5%, water adds to 100%.Mill is added in the continuous phase containing triticonazole The suspended phase of very thin Picarbutrazox is obtained, 20% Picarbutrazox triticonazole suspension emulsions are mixed to prepare.

Embodiment 12：30% Picarbutrazox triticonazole suspension emulsions

Picarbutrazox 20%, triticonazole 10%, naphthalene sulfonic acid-formaldehyde condensation product 7%, spaonin powder 3%, phenolic resin 1.1%, three is sweet Alcohol 2%, silicone 0.1%, agriculture breast 400#6%, water adds to 100%, is added in the continuous phase containing triticonazole finely ground The suspended phase of Picarbutrazox, is mixed to prepare 30% Picarbutrazox triticonazole suspension emulsions.

Embodiment 13：12.5% Picarbutrazox triticonazole microemulsions

Picarbutrazox10%, triticonazole 2.5%, N- methyl pyrrolidones 6%, oxirane-propylene oxide block copolymerization Thing 4%, alkyl biphenyl ether disulfonic acid magnesium salts 5%, silicone compound 0.4%, toluene 3%, propane diols 2%, epoxychloropropane 1.1%, Deionized water adds to 100%, is made 22.5% Picarbutrazox triticonazole microemulsions.

Embodiment 14：5% Picarbutrazox triticonazole microemulsions

Picarbutrazox4%, triticonazole 1%, cyclohexanone 5%, agriculture breast 500#4%, Nongru-700 #5%, C_8~10Aliphatic alcohols 0.2%, Ethyl acetate 2.5%, polyethylene glycol 2%, tributyl phosphate 1.4%, deionized water add to 100%, are made 5% Picarbutrazox triticonazole microemulsions.

Embodiment 15：50% Picarbutrazox triticonazole water dispersible granules

Picarbutrazox30%, triticonazole 20%, octylphenol polyethylene oxyethylene group ether sulfate 1%, neopelex 1%, Sodium carbonate 1%, white carbon adds to 100%, and aforementioned formula material is uniformly mixed, with micro jet, through mediating, being subsequently adding Granulated in fluidized-bed granulation dryer, dried, sieve after through sampling analysis i.e. be obtained 50% Picarbutrazox sterilize Azoles water dispersible granules.

Embodiment 16：10% Picarbutrazox triticonazole aqueous emulsions

Picarbutrazox8%, triticonazole 2%, 600# phosphates 4%, propyl alcohol 7%, agriculture breast 600#3%, xanthans 0.7%, propane diols 2.4%th, toluene 4%, deionized water adds to 100%, is made 10% Picarbutrazox triticonazole aqueous emulsions.

Embodiment 17：12% Picarbutrazox triticonazole missible oil

Picarbutrazox 10%, triticonazole 2%, ethoxylated castor oil 5%, calcium dodecyl benzene sulfonate 3%, SOLVESSO^TM200 add to 100%, stir to transparent homogeneous phase is obtained, and obtain 12% Picarbutrazox triticonazoles breast Oil.

Embodiment 18：12%Picarbutrazox triticonazole microcapsule suspension-suspendinagent agents

Picarbutrazox 10%, triticonazole 2%, Synperonic PE/64 15%, citric acid 0.05%, water 10%, PAPI 20%, SOLVESSO^TM100 5%, dispersant LFH0.15%, defoamer 0.16%, urea 5.5%；Water complements to 100%； By PAPI, triticonazole, SOLVESSO^TM100 oil phases for being formed are added in the aqueous solution containing Synperonic PE/64, form emulsus Liquid.Then heat and be incubated and add catalyst reaction 2 hours at 50 DEG C.The microcapsules of triticonazole is obtained after cooling. Synperonic PE/64, dispersant LFH, defoamer, urea, Picarbutrazox and water are mixed in proportion, and through sand Mill, is prepared into suspending agent.The triticonazole microcapsules that will be obtained is added in the suspending agent of Picarbutrazox, is uniformly mixing to obtain 12%Picarbutrazox triticonazole microcapsule suspension-suspendinagent agents.

The 38%Picarbutrazox triticonazole seed coat agents of embodiment 19

Picarbutrazox 10%, triticonazole 28%, AEO sulfosuccinic acid monoesters disodium 10%, innovation wood Quality sulfoacid calcium 5%, xanthans 1%, bentonite 1%.Glycerine 5%,

PVP-K30 1%, complements to 100%, and above-mentioned each component is mixed in proportion, and through being sanded, is prepared into 38% Picarbutrazox triticonazole seed coat agents.

The 50%Picarbutrazox triticonazole coated granules of embodiment 20

Picarbutrazox 20%, triticonazole 30%, polyethylene glycol 3%, the silicic acid 1% of high degree of dispersion,

Calcium carbonate complements to 100%, in a mixer, by levigate active component even spread to the load soaked by polyethylene glycol On body, the dust-free coated granules agent of 50%Picarbutrazox triticonazoles can be obtained in this way.

Be well mixed for Picarbutrazox 70% and triticonazole 30% by embodiment 21.

The just Picarbutrazox 40% of embodiment 22 and triticonazole 60% are well mixed.

Above example proportioning is weight per distribution ratio.

Biological test example：

The combination of active principles of different agricultural chemicals is made agricultural chemicals, is exploitation at present and development novel pesticide and preventing and treating agriculturally resistance A kind of effective and quick way of germ.After the pestsides synthesis of different cultivars, three kinds of type of action are typically exhibited：It is added and makees With, synergistic effect and antagonism.But which kind of effect be specially, it is impossible to predict, only just be can know that by many experiments.Compounding Synergy is formulated well, due to significantly improving actual prevention effect, reduces the usage amount of agricultural chemicals, so as to greatly delay The generation of resistance.

First, virulence test：

The toxicity index of each medicament and the co-toxicity coefficient of mixture are calculated according to the abundant methods of Sun Yun（CTC values）, when CTC≤80, then combine Thing shows antagonism, when 80<CTC<120, then composition show summation action, when CTC >=120, then composition performance Go out synergistic effect.

Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

B in the percentage composition+B medicament toxicity index * mixtures of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures Percentage composition

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixture theoretical toxicity index (TTI) * 100

Experiment 1：The toxicity test of cucumber downy mildew

Selected from the cucumber seedling that growing way is consistent, sprayed under 50PSI pressure with potter spray towers, per basin about 5mL, each medicine Agent sets 12 concentration gradients.24h connects bacterium after chemicals treatment, and the cucumber downy mildew leaf that will pick up from field is uniform above cucumber seedling Shake off conidium to be inoculated with, then cucumber seedling is put into greenhouse and is cultivated.Morbidity after 7d according to cucumber downy mildew is classified Standard complete stool investigates disease index, and calculates prevention effect, and concentration EC50 in suppressing then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).

Table 1

As known from Table 1, Picarbutrazox and triticonazole are preventing the weight proportion 50 of cucumber downy mildew:1-1:50 scope In when, co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 2：The toxicity test of tomato blight

Selected from the tomato seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the tomato in field Epidemic disease leaf is uniformly shaken off conidium above tomato seedling and is inoculated with, and then tomato seedling is put into greenhouse and is cultivated.Pressed after 7d Morbidity grade scale complete stool according to tomato blight investigates disease index, and calculates prevention effect, is then calculated with least square method Concentration EC50 in suppression, then calculate co-toxicity coefficient (CTC) according to the abundant methods of Sun Yun.

Table 2：Virulence test result of the present invention on preventing and treating tomato blight

As known from Table 2, Picarbutrazox and triticonazole are preventing the weight proportion 50 of tomato blight:1-1:In 50 scope When, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect.

Experiment 3：The toxicity test of Penicillium italicum

Using suppression mycelial growth rate method：Experiment target is designated as Penicillium italicum.

By Picarbutrazox and triticonazole respectively use acetone solution, then with 0.1% the Tween-80 aqueous solution dilute match somebody with somebody The liquid of series concentration is made, 6mL to the conical flask of sterilizing is drawn respectively in superclean bench, 50 DEG C or so of horse is added Bell potato glucose agar medium（PDA）54mL, pours into 4 plates of diameter 9cm after shaking up, be made containing for 4 respective concentrations Malicious culture medium；The Picarbutrazox of different ratio and triticonazole series concentration compound solution are made and contained with same method Malicious culture medium.The Penicillium italicum bacterium of 2 days will be cultivated, bacterium block is broken into colony edge with the card punch of diameter 5mm, use transfer needle Bacterium block is moved to the toxic PDA culture medium center being configured in advance, culture in 25 DEG C of incubators is subsequently placed in, often treatment repeats 4 It is secondary.After 3 days, each treatment colony diameter cm is measured using crossing method slide calliper rule, obtain correction and suppress percentage.Each bacterium colony Two diameters are surveyed in right-angled intersection, and bacterium colony size is represented with its average.Then colony growth inhibiting rate is obtained as the following formula：

Then concentration EC in suppressing is calculated with least square method₅₀, then calculate co-toxicity coefficient according to the abundant methods of Sun Yun（CTC）.

Table 3：To the virulence test result of Penicillium italicum

As known from Table 3, the combination preventing and treating Penicillium italicum of Picarbutrazox and triticonazole is in proportioning 50:1-1:50 scope In when, co-toxicity coefficient is all higher than 120, illustrates that both show as gain effect at mixture in the range of this.

Experiment 4：The toxicity test of maize head smut

Selected from the maize seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the corn in field Sick leaf is uniformly shaken off conidium above maize seedling and is inoculated with, and then maize seedling is put into greenhouse and is cultivated.After 7d according to The morbidity grade scale complete stool investigation disease index of maize head smut, and prevention effect is calculated, then use least square method meter Concentration EC50 in suppressing is calculated, then co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.

Table 4：Virulence test result of the present invention on preventing and treating maize head smut

As known from Table 4, Picarbutrazox and triticonazole are preventing the weight proportion 50 of maize head smut:1-1:50 model When enclosing inner, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect.

Test 5 leaf blights（Wheat）Toxicity test

Selected from the wheat seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the wheat in field Leaf spoting bacteria is uniformly shaken off conidium above wheat seedling and is inoculated with, and then wheat seedling is put into greenhouse and is cultivated.After 7d Morbidity grade scale complete stool according to wheat leaf blight investigates disease index, and calculates prevention effect, then uses least square method Concentration EC50 in suppressing is calculated, then co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.

Table 5：Virulence test result of the present invention on preventing and treating wheat leaf blight

As known from Table 5, Picarbutrazox and triticonazole prevention wheat leaf blight, in weight proportion 50:1-1:In 50 scope When, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect.

Test 6 powdery mildews（Wheat）Toxicity test

Selected from the wheat seedling that growing way is consistent, each treatment supplies examination leaf seedling from 3 basins, with potter spray towers under 50PSI pressure Spraying, per basin about 5mL, each medicament sets 12 concentration gradients.24h connects bacterium after chemicals treatment, will pick up from the wheat in field Powdery mildew leaf is uniformly shaken off conidium above wheat seedling and is inoculated with, and then wheat seedling is put into greenhouse and is cultivated.After 7d Morbidity grade scale complete stool according to wheat powdery mildew investigates disease index, and calculates prevention effect, then uses least square method Concentration EC50 in suppressing is calculated, then co-toxicity coefficient (CTC) is calculated according to the abundant methods of Sun Yun.

Table 6：Virulence test result of the present invention on preventing and treating wheat powdery mildew

As known from Table 6, Picarbutrazox and triticonazole prevention wheat powdery mildew, in weight proportion 50:1-1:In 50 scope When, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect.

Experiment 7：The toxicity test of peanut pythium spp

Selected from the fresh branches and leaves of peanut seedling that growing way is consistent, sprayed under 50PSI pressure with potter spray towers, per basin about 5mL, each medicine Agent sets 12 concentration gradients.24h connects bacterium after chemicals treatment, and the peanut pythium spp disease leaf that will pick up from field is equal above fresh branches and leaves of peanut seedling It is even shake off conidium be inoculated with, then fresh branches and leaves of peanut seedling is put into greenhouse and is cultivated.According to the morbidity point of peanut pythium spp after 7d Level standard complete stool investigation disease index, and prevention effect is calculated, concentration EC50 in then being suppressed with least square method calculating, then according to The abundant methods of Sun Yun calculate co-toxicity coefficient (CTC).

Table 7：Virulence test result of the present invention in preventing and treating peanut pythium spp

As known from Table 7, Picarbutrazox and triticonazole preventing and treating peanut pythium spp, in weight proportion 50:1-1:In 50 scope When, co-toxicity coefficient is all higher than 120, illustrates that both mixtures in the range of this show as gain effect.

2nd, the evaluation of pesticide effectiveness：

When the effect of active compound combinations exceedes the summation of the effect when each reactive compound is administered alone, there is association Same synergistic effect.The predictive role of two kinds of particular combinations of reactive compound can be used so-called " Colby formula " (referring to S.R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations ", Weeds 1967,15,20-22) it is calculated as below:If

X is the activity when the reactive compound A that consumption is mg/ha or concentration is mppm is used；

Y is the activity when the reactive compound B that consumption is ng/ha or concentration is nppm is used, and is expressed as accounting for untreated right According to percentage；

E is the activity when the reactive compound A and B that consumption is m and n g/ha or concentration is m and n ppm is used,

So

If the activity of actual observation（O）More than expection activity（E）, then said composition has synergistic effect.

Following biological test example is used to illustrate the present invention.But, the present invention is not limited to these embodiments.

Test the test of 8 epidemic diseases（Tomato）The efficacy testing of/protectiveness

Solvent：24.5 pbw acetones

24.5 weight portion dimethylacetylamides

Emulsifying agent：1 weight portion alkylaryl polyglycol ether

In order to obtain the appropriate formulation of reactive compound, 1 Parts by weight Active compound is mixed with a certain amount of solvent and emulsifying agent Close, dilute with water concentrate to required concentration.

In order to test protectiveness activity, young plant is sprayed with certain rate of application with active agent preparations.In sprinkling Coating dry after, plant is inoculated with the aqueous spore suspension of phytophthora infestans.Then the plant is placed on about 20 DEG C, in the incubate box that relative atmospheric humidity is 100%.

3 days after incubation, test result is evaluated.0% represents the drug effect equivalent to control sample, 100% drug effect Indicate and occur without illness.

Upper table clearly illustrates that, according to bactericidal composition of the invention, Picarbutrazox is with the weight ratio of triticonazole 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of tomato blight in the range of 50, that is, there is obvious collaboration effect Should.

Test the test of 9 downy mildew（Grape）The efficacy testing of/protectiveness

Solvent：24.5 pbw acetones

24.5 weight portion dimethylacetylamides

Emulsifying agent：1 weight portion alkylaryl polyglycol ether

In order to test protectiveness activity, young plant is sprayed with certain rate of application with active agent preparations.In sprinkling Coating dry after, plant is inoculated with the aqueous spore suspension of Plasmopara viticola.Then the plant is placed on About 20 DEG C, in the incubate box that relative atmospheric humidity is 100%.

Upper table clearly illustrates that, according to bactericidal composition of the invention, Picarbutrazox is with the weight ratio of triticonazole 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of downy mildew of garpe in the range of 50, that is, there is cooperative effect, and And the effect of cooperative effect is the most obvious.

Experiment 10:Antiseptic effect to preserving oranges and tangerines

Treatment oranges and tangerines picking time plucks fruit, rejects disease fruit and wound fruit, and fruit 1min is soaked with treatment liquid.Control clear water dipping.Respectively Dried after treatment leaching fruit, next day loads the plastic box that surrounding is lined with newspaper, normal temperature laboratory storage.Check decayed fruit number within 7 days after treatment And decayed fruit is rejected, while having weighed up fruit weight.And calculate preventive effect.

Upper table clearly illustrates that, according to bactericidal composition of the invention, Picarbutrazox is with the weight ratio of triticonazole 50:1-1:In the range of 50, to the anti-corrosion of storage period oranges and tangerines, actual drug effect is higher than the drug effect being calculated, i.e., in the presence of obvious Cooperative effect.

Experiment 11：Maize head smut/curative efficacy testing

Solvent：24.5 pbw acetones

24.5 weight portion dimethylacetylamides

Emulsifying agent：1 weight portion alkylaryl polyglycol ether

In order to test therapeutical active, the maize seedling to the 5-6 leaf phases in seedling-growing container is inoculated with the spore of Sporisorium reilianum Sub- water slurry, places 18 hours in the incubate box that 25 DEG C, relative atmospheric humidity are 100%.After air-drying leaf, with activation Compound preparation sprays plant with certain rate of application, then it is fallen ill in greenhouse.Inoculation investigates occurring degree after 10 days. 0% represents the drug effect equivalent to control sample, and 100% drug effect indicates occur without illness.

Upper table clearly illustrates that, according to bactericidal composition of the invention, Picarbutrazox is with the weight ratio of triticonazole 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of maize head smut in the range of 50, that is, there is cooperative effect, And the effect of cooperative effect is the most obvious.

Experiment 12：Powdery mildew is tested（Wheat）/ curative efficacy testing

Solvent：24.5 pbw acetones

24.5 weight portion dimethylacetylamides

Emulsifying agent：1 weight portion alkylaryl polyglycol ether

In order to test therapeutical active, the wheat seedling to the 5-6 leaf phases in seedling-growing container is inoculated with the spore of wheat powdery mildew Water slurry, places 18 hours in the incubate box that 25 DEG C, relative atmospheric humidity are 100%.After air-drying leaf, active ingredient is used Thing preparation sprays plant with certain rate of application, then it is fallen ill in greenhouse.Inoculation investigates occurring degree after 10 days.0% The drug effect equivalent to control sample is represented, 100% drug effect indicates occur without illness.

Upper table clearly illustrates that, according to bactericidal composition of the invention, Picarbutrazox is with the weight ratio of triticonazole 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of wheat powdery mildew in the range of 50, that is, there is cooperative effect, and And the effect of cooperative effect is the most obvious.

Experiment 13：Pythium spp is tested（Cotton）The efficacy testing of/protectiveness

Solvent：24.5 pbw acetones

24.5 weight portion dimethylacetylamides

Emulsifying agent：1 weight portion alkylaryl polyglycol ether

In order to test therapeutical active, the fresh branches and leaves of peanut seedling to the 3-5 leaf phases in seedling-growing container is inoculated with the spore fluid of peanut pythium spp Suspension, places 18 hours in the incubate box that 25 DEG C, relative atmospheric humidity are 100%.After air-drying leaf, reactive compound is used Preparation sprays plant with certain rate of application, then it is fallen ill in greenhouse.Inoculation investigates occurring degree after 10 days.0% table Show the drug effect equivalent to control sample, 100% drug effect indicates occur without illness.

Upper table clearly illustrates that, according to bactericidal composition of the invention, Picarbutrazox is with the weight ratio of triticonazole 50:1-1:It is higher than the drug effect being calculated to the actual drug effect of peanut pythium spp in the range of 50, that is, there is cooperative effect, and And the effect of cooperative effect is the most obvious.

Claims

1. a kind of bactericidal composition, it is characterised in that：The bactericidal composition includes active component Picarbutrazox and sterilizing The weight ratio of azoles, Picarbutrazox and triticonazole is 50:1-1:50, preferably 25:1-1:25, more preferably 10:1-1: 10, more preferably 5:1-1:5.

2. bactericidal composition according to claim 1, it is characterised in that：The Picarbutrazox and triticonazole quality Sum accounts for the 5%-90% of the Bactericidal composite amount of substance, more preferably more preferably 10%-80%, 20%-60%.

3. bactericidal composition according to claim 1, it is characterised in that：The formulation of the bactericidal composition be suspending agent, Seed coat agent, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion granule, missible oil, microemulsion, water Emulsion, effervescent tablet, ultra low volume liquids, suspension emulsion.

4. bactericidal composition according to claim 1, it is characterised in that：Also include filler and/or surfactant.

5. it is a kind of prevent and treat plant pathogen method, it is characterised in that：Bactericidal composition described in claim 1 is acted on into cause In germ and/or its environment, or plant, seed, soil, region, material or space.

6. method according to claim 5, it is characterised in that：By the Picarbutrazox described in claim 1 and sterilizing Azoles is administered simultaneously or applies respectively or sequential application.

7. the bactericidal composition described in claim 1 is used to prevent and treat the purposes of fungi and bacterium.

8. the bactericidal composition described in claim 1 is used to prevent and treat the purposes of fungi and bacterium in cereal, water fruits and vegetables.

9. the bactericidal composition described in claim 1 is used to protect the use of plant propagation material and the plant organ for then growing On the way.

10. caused a disease in the place preventing and treating soil or cultivation medium of preventing and treating needed for the bactericidal composition described in claim 1 is applied to Or saprophytic fungi and the purposes of bacterium.

Bactericidal composition described in 11. claims 1 is used to prevent and treat the purposes of fruit vegetables storing period disease.

The plant pathogenic fungi of a kind of 12. control plants, plant part, plant propagation material and the plant organ for then growing Method, it is characterised in that：The method is included the bactericidal composition described in claim 1 with agronomy effectively and substantially without plant poison The amount of application of property is applied with Seed Treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution or smoke Etc. method be administered to plant, plant part, plant propagation material or plant growing or needing the soil that grows wherein or In cultivation medium.