CN103988843B - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
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- CN103988843B CN103988843B CN201410233932.0A CN201410233932A CN103988843B CN 103988843 B CN103988843 B CN 103988843B CN 201410233932 A CN201410233932 A CN 201410233932A CN 103988843 B CN103988843 B CN 103988843B
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Abstract
The present invention relates to a kind of bactericidal composition, further relate to a kind of method preventing and kill off plant disease。The invention provides a kind of bactericidal composition, comprising bellkute and of pyrimethanil active component, bellkute and of pyrimethanil components by weight percentage is the effect obtaining Synergistic within the scope of 100:1-1:100。The content of bellkute and of pyrimethanil active component accounts for the 5%-60% of compositions。The present invention is by carrying out binary built by bellkute and of pyrimethanil, and both mechanisms of action are different, and no interactions resistance, and mixing use can expand fungicidal spectrum, play better prevention effect, and delays pathogen to develop immunity to drugs, and various crop is all comparatively safe。Can be used for preventing and treating the purposes of fungus on Cereal, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant。
Description
Technical field
The present invention relates to a kind of bactericidal composition, further relate to a kind of method preventing and kill off plant disease。
Background technology
Due to the harm of various phytopathogens, making many crops bring about great losses, the lighter's underproduction, severe one may result in total crop failure, directly affects the economic interests of peasant, therefore forces peasant to have to use antibacterial to carry out controlling disease。
Agricultural crops (Cereal, melon fruits and vegetables, flowers) is upper often there is multiple fungal disease, such as gray mold, powdery mildew, sclerotiniose, stem wilt, blight dis-ease, anthrax, ring spot, scab, leaf spot, spot defoliation, fruit soft rot, penicilliosis, green mould, rust, peach brown rot, smut, cecospora spot etc.。
Bellkute (Bellkute), has another name called biguanide three octyl benzene sulfonate, and its chemical name is: 1 ＇ 1-imino group (octyl group methylene) biguanide 3(alkyl benzene sulfonate), it is the antistaling disinfectant of the single molecular structure of biguanide salt, its structural formula is:
N=10～13
Bellkute is produced by Cao Da company of Japan。Bellkute tagging and prevention type antibacterial as broad spectrum activity, local permeability is strong, some pathogenic fungi there is significantly high growth inhibitory activity, the biosynthesis of Mycophyta ester compounds and cell membrane function are mainly worked by it, suppress the formation of spore germination, germ tube elongation, appresorium and mycelia, the fungal disease caused by most of ascomycetess and Fungi Imperfecti can be prevented and treated。The disease such as fungal disease, particularly water fruits and vegetables and the gray mold of multiple kinds of crops, powdery mildew, sclerotiniose, stem wilt, blight dis-ease, anthrax, ring spot, scab, leaf spot, spot defoliation, penicilliosis, green mould and fruit soft rot that bellkute can be widely used for preventing and treating most of ascomycetes and Fungi Imperfecti causes。Especially to Fructus Lycopersici esculenti, grape grey mould, apple peels and cores, powdery mildew of cucumber, watermelon grafting all have good preventive effect。
Of pyrimethanil (Pyrimethanil), chemical name: N-(4,6-dimethyl pyrimidine-2-base) aniline, structural formula is:
Of pyrimethanil is conventional sterilants, belongs to anilino-pyrimidine。The of pyrimethanil mechanism of action is unique, by the secretion of suppression infection process enzyme thus stoping infecting and killing pathogenic bacteria of pathogenic bacteria。The ash arrhizus bacteria that conventional non-anilino-pyrimidine (benzimidazole and carbamic acid lipid) antibacterial has been developed immunity to drugs has specially good effect, the main germ tube elongation suppressing Botrytis cinerea and mycelial growth, also have certain inhibitory action to the spore rudiment of Botrytis cinerea in certain administration time。There is Uptake and translocation and fumigation action simultaneously, it is rapidly reached the spraying such as the flower of plant, the young fruit position that is beyond one's reach after dispenser and kills pathogenic bacteria, after halogen family specially good effect penetrating agent, can increase and adhere to time and seepage velocity at blade and fruit, be conducive to absorb, make drug effect faster, more stable。Of pyrimethanil can be used for preventing and treating Fructus Cucumidis sativi, Fructus Lycopersici esculenti, Fructus Vitis viniferae, Fructus Fragariae Ananssae, Semen Pisi sativi, Folium Allii tuberosi, etc. crop gray mold, droop and black spot of fruit tree, spot defoliation etc.。
But when practical application, long-term single administration one reactive compound carrys out controlling disease can cause that disease drug resistance produces, and causes compound preventive effect to decline, even thoroughly loses preventive effect。In order to reduce the risk that phytopathogen develops immunity to drugs, application Synergistic bactericidal composition prevent and treat noxious plant pathogenic fungi be at present frequently with one of way。
Summary of the invention
It is an object of the present invention to provide a kind of bactericidal composition, be a kind of bactericidal composition with synergistic function。It is a further object to provide a kind of method preventing and kill off plant disease。
The present invention, through various further investigations, found that the bactericidal composition containing bellkute and of pyrimethanil, can prevent and treat the fungal disease of various vegetables, fruit and cereal crop。Bactericidal composition containing bellkute and of pyrimethanil is not only for common phytopathogen, and the pathogen that those have produced drug resistance is also highly effective, also has excellent prevention effect, thus complete the present invention under disease recurs condition。
A kind of bactericidal composition of the present invention is to take techniques below scheme to realize:
A kind of bactericidal composition, comprises bellkute and of pyrimethanil as active component, and the weight ratio of described bellkute and of pyrimethanil is 100:1-1:100。
Bellkute and of pyrimethanil can, namely together or separate administration, or be used successively simultaneously；The result of prophylactico-therapeutic measures is not generally had any impact by the order in separate administration situation。
Compound bellkute and of pyrimethanil are generally with 100:1-1:100, it is preferable that 50:1-1:50, more preferably 25:1-1:25, more preferably 10:1-1:10, and further preferably the weight ratio of 5:1-1:5 is used。
A kind of bactericidal composition, comprises bellkute and of pyrimethanil active component, filler and/or surfactant。
Described bactericidal composition dosage form is wettable powder, cream, suspending agent, suspension emulsion, microcapsule, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foam, unguentum, aerosol, ultra-low volume spray liquid。
Described bactericidal composition, wherein accounts for the 5%-90% of bactericidal composition containing the content of bellkute and of pyrimethanil active component。
Described bactericidal composition, wherein accounts for the 10%-80% of bactericidal composition containing the content of bellkute and of pyrimethanil active component。
Described bactericidal composition, wherein accounts for the 20%-60% of bactericidal composition containing the content of bellkute and of pyrimethanil active component。
A kind of method preventing and kill off plant disease, is applied to plant, plant propagation material and the organ grown subsequently, environment by described bactericidal composition。
Described compositions-treated needs the propagating materials of plant to be protected。
The plant propagation material of described compositions-treated。
Described bactericidal composition is for preventing and treating the purposes of fungus。
Described bactericidal composition is for preventing and treating the purposes of fungus on Cereal, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant。
Described bactericidal composition is used for preventing and treating gray mold, sclerotiniose, leaf spot, banded sclerotial blight, full rot, rust, anthrax, early blight, powdery mildew, scab, melasma, blight dis-ease, spot defoliation, stem wilt etc.。
Described bactericidal composition is for preventing and treating the purposes of fruit vegetables storing period disease。
Described bactericidal composition can coating, spraying, immersion, dense fog, the mode such as broadcast sowing or pour and use。
Described bellkute and of pyrimethanil compound are used simultaneously or are used respectively or sequential application。
Detailed Description Of The Invention
The synergy of bellkute and of pyrimethanil, the weight ratio of reactive compound can select in relatively larger scope。The weight ratio of bellkute and of pyrimethanil is 100:1-1:100, or 50:1-1:50, or 20:1-1:20, it is preferable that 10:1-1:10, more preferably 5:1-1:5。
The bactericidal composition of the present invention, the content of active component depends on the dosage sterilized when being used alone, and also depends on mixed ratio and the potentiation degree of a kind of antibacterial and another kind of antibacterial。
In described bactericidal composition, the content of bellkute and of pyrimethanil active component accounts for the 5%-90% of bactericidal composition, it is preferable that 10%-80%, more preferably 20%-60%。
In described bactericidal composition, it is possible to only comprise active component, it is also possible to comprise filler and/or surfactant。
The bactericidal composition of the present invention, it is possible to prepare into various dosage form, for instance wettable powder, cream, suspending agent, suspension emulsion, microcapsule, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foam, unguentum, aerosol, ultra-low volume spray liquid。
According to the present invention, term " filler " refers to can be combined with reactive compound or combine to make it easier for be administered to the naturally occurring or synthetic organic or inorganic compound of object (such as plant, crop or grass class)。Therefore, described filler is preferably inertia, at least should be agricultural acceptable。Described filler can be solid or liquid。
Liquid filler is generally: water, alcohol type (such as methanol, ethanol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, Ketohexamethylene etc.), ethers (such as ether, dioxanes, methylcellulose, oxolane etc.), aliphatic hydrocarbon class (such as kerosene, mineral wet goods), aromatic hydrocarbons class (such as benzene, toluene, dimethylbenzene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbon, chlorobenzene, Deng), halogenated hydrocarbon class, amide-type, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal wet goods。
Solid-filling agent is generally: phyteral powdery type (the such as granule of Semen sojae atricolor powder, starch, grain dust, wood powder, bark powder, sawdust, walnut shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem, extract the residue etc. after plant essence), paper, sawdust, pulverize the synthesized polymer body of synthetic resin etc., clay class (such as Kaolin, Bentonite, acid china clay etc.), Pulvis Talci class。Silica (such as kieselguhr, silica sand, Muscovitum, moisture silicic acid, calcium silicates), activated carbon, natural mineral matter class (Pumex, attapulgite and zeolite etc.), fire the chemical fertilizer of the inorganic mineral powder of kieselguhr, sand, plastics medium etc. (such as polyethylene, polypropylene, polyvinylidene chloride etc.), potassium chloride, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, carbamide, greening ammonium etc., clay fertilizer, these materials can be used alone or two or more is used with。
For making effective ingredient compound emulsifying, dispersion, solubilized, and/or moistening can use surfactant such as can enumerate fatty alcohol-polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, the phosphate ester of polyoxyethylene alcohol or phenol, the fatty acid ester of polyhydric alcohol, alkyl aryl sulphonic acid, naphthalene sulfonic acid polymer, lignosulfonates, a shape copolymer of macromolecule comb shape, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, oils and fats, fatty alcohol and ethylene oxide condensate, the polyacrylates such as alkyltaurate, protein hydrolysate。Suitable oligosaccharide thing or polymer, for instance based on the combination of independent vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine。
Preparation it be also possible to use viscosifier such as carboxymethyl cellulose and the natural and synthetic polymer of powder, granule or latex form, for instance Radix Acaciae senegalis, polyvinyl alcohol and polyvinyl acetate or natural phospholipid, for instance cephalin and lecithin, and synthetic phospholipid。Other additive is mineral oil and vegetable oil。
It is likely to the coloring agent such as inorganic pigment used, for instance, ferrum oxide, titanium oxide and Prussian blue, and organic dyestuff, such as alizarin dyes, azo dye or metal phthalein mountain valley with clumps of trees and bamboo dyestuff, and trace nutrient, such as ferrum, manganese, boron, ketone, cobalt, molybdenum and zinc salt。
The disintegrating agent being likely to use is selected from: one or more in bentonite, carbamide, ammonium sulfate, aluminum chloride, citric acid, succinic acid, sodium bicarbonate。
The stabilizer being likely to use is selected from: the one in sodium citrate, resorcinol。
The antifreezing agent being likely to use is selected from: one or more in ethylene glycol, propylene glycol, glycerol, carbamide。
Described defoamer is selected from: silicone oil, silicone compound, C10-20Saturated fat acid compounds, C8-10One or more in aliphatic alcohols compound。
Optionally, other annexing ingredient can also be comprised, for instance protecting colloid, binding agent, thickening agent, thixotropic agent, penetrating agent, stabilizer, screening agent。
Described active component can be mixed with filler and/or surfactant and prepare by known way by the described preparation of the present invention, also can add other conventional additives, such as drier and coloring agent, stabilizer, pigment, defoamer, preservative, thickening agent etc.。
The bactericidal composition of the present invention with other medicament with weeding, parasite killing or bactericidal property, particularly can also mix use, it is also possible to mix use with insecticide, protective agent, growth regulator, nutrient for plants or soil conditioner with protective fungicide。
The bactericidal composition of the present invention has extraordinary fungicide performance, and can be used for preventing and treating phytopathogenic fungi, described fungus is especially selected from Ascomycetes (Ascomycetes), Basidiomycetes (Basidiomycetes), Phycomycetes (Phycomycetes) and deuteromycetes (Deuteromycetes) fungus。
The bactericidal composition of the present invention is particularly suited for preventing and treating following plant disease:
Rod method (AIternaria) on-vegetable, oilseed rape, sugar beet, fruit and rice belongs to,
Silk capsule mould (Aphanomyces) on-sugar beet and vegetable belongs to,
Bipolaris (Bipolaris) in-Semen Maydis, Cereal, rice and lawn belongs to and the compacted spore of interior umbilicus (Drechslera) belongs to,
Standing grain powdery mildew (Blumeriagraminis) (powdery mildew) on-Cereal,
Botrytis cinerea (Botrytiscinerea) (gray mold) on-Fructus Fragariae Ananssae, vegetable, flowers and vine,
Caulis et Folium Lactucae sativae dish on-Caulis et Folium Lactucae sativae obstructs mould (Bremialactucae),
Tail spore (Cercospora) on corn and soybean, rice and sugar beet belongs to,
Cochliobolus (Cochliobolus) on-Semen Maydis, Cereal, rice belongs to (the standing grain rotation spore on such as Cereal
Chamber bacterium (Cochliobolussativus), palace portion cochliobolus (Cochliobolusmiyabeanus) on rice),
Thorn dish spore (Colletotricum) on-Semen sojae atricolor and Cotton Gossypii belongs to,
The compacted spore of interior umbilicus (Drechslera) on-Cereal and Semen Maydis belongs to,
The compacted spore of prominent umbilicus (Exserohilum) on-Semen Maydis belongs to,
. two spore powdery mildews (Erysiphecichoracearum) on Fructus Cucumidis sativi and monofilament shell powdery mildew
(Sphaerothecafuliginea),
Fusarium (Fusarium) in-each kind of plant belongs to and wheel branch spore (Verticillium) belongs to,
Gaeumannomyce (Gaeumanomycesgraminis) on-Cereal belongs to,
Red mould (Gibberella) on-Cereal and rice belongs to the (gibberella fujikuroi (Gibberella on such as rice
fujikuroi)),
Gram’s staining coordination compound (Grainstainingcomplex) on-rice,
The compacted spore of length (Helminthosporium) on-Semen Maydis and rice belongs to,
Michrodochiumnivale on-Cereal,
Spherical cavity bacterium (Mycosphaerella) on-Cereal, Fructus Musae and Semen arachidis hypogaeae belongs to,
Phakopsora pachyrhizi (Phakopsorapachyrhizi) on-Semen sojae atricolor and mountain horse sulphur layer rest fungus
(Phakopsarameibomiae),
Phomopsis (Phomopsis) on-Semen sojae atricolor and Helianthi belongs to,
Phytophthora infestans (Phytophthorainfestans) on-Rhizoma Solani tuber osi and Fructus Lycopersici esculenti,
The raw single shaft of Fructus Vitis viniferae on-vine mould (Plasmoparaviticola),
Apple mildew bacterium (Podosphaeraleucotricha) on-Fructus Mali pumilae,
Semen Tritici aestivi Phyllostachys pubescens (Pseudocercosporellaherpotrichoides) on-Cereal,
False downy mildew (Pseudoperonospora) on-Flos lupuli (Flos Humuli Lupuli) and calabash belongs to,
Handle rest fungus (Puccinia) on-Cereal and Semen Maydis belongs to,
Nuclear cavity bacteria (Pyrenophora) on-Cereal belongs to,
Pyricularia oryzae (Pyricularia0 η zae) on-rice, wood photovoltaicing leather bacteria (Corticiumsasakii), broom stalk Cylindrocarpon (Sarocladiumoryzae), sheath rot of rice plant (S.attenuatum), rice leaf smut (Entylomaoryzae)
Piricularia oryzae (Pyriculariagrisea) on-lawn and Cereal,
-lawn, rice, Semen Maydis, Cotton Gossypii, oilseed rape, Helianthi, sugar beet, vegetable and other plant
Corruption mould (Pythium) on thing belongs to,
-Cotton Gossypii, rice, Rhizoma Solani tuber osi, lawn, Semen Maydis, oilseed rape, Rhizoma Solani tuber osi, sugar beet, vegetable and its
Rhizoctonia (Rhizoctonia) on his plant belongs to,
Sclerotinite (Sclerotinia) in-oilseed rape and Helianthi belongs to,
Wheat septoria (Septoriatritici) on-Semen Tritici aestivi and the many spore (Stagonospora of the withered shell of grain husk
nodorum),
Fructus Vitis viniferae snag shell (Erysiphe) on-vine,
Setospaeria on-Semen Maydis and lawn belongs to,
Silk axle smut (Sphacelothecareilinia) on-Semen Maydis,
Thielaviopsis sp (Thievaliopsis) on-Semen sojae atricolor and Cotton Gossypii belongs to,
Tilletia foetida (Tilletia) on-Cereal belongs to,
Smut (Ustilago) on-Cereal, Semen Maydis and sugar beet belongs to,
Black star bacterium (Venturia) on-Fructus Mali pumilae and pears belongs to (scab)。
The bactericidal composition of the present invention is even more important to preventing and treating a large amount of fungus in various crops such as Cotton Gossypii, vegetable variety (such as Fructus Cucumidis sativi, beans, Fructus Lycopersici esculenti, Rhizoma Solani tuber osi and cucurbitaceous plant), Fructus Hordei Vulgaris, dogstail, Herba bromi japonici, Fructus Musae, coffee, Semen Maydis, fruit variety, rice, rye (Secale cereale L.), Semen sojae atricolor, vine, Semen Tritici aestivi, ornamental plant, Semen arachidis hypogaeae, Brassica campestris L, Caulis Sacchari sinensis and various crop seed。
The bactericidal composition of the present invention is particularly suitable for preventing and treating following plants pathogenic epiphyte: the standing grain powdery mildew (Blumeriagraminis) (powdery mildew) in Cereal, two spore powdery mildews (Erysiphecichoracearum) in cucurbitaceous plant and monofilament shell (Sphaerothecafuliginea), apple mildew bacterium (Podosphaeraleucotricha) in Fructus Mali pumilae, Fructus Vitis viniferae snag shell (Uncinulanecator) in vine, handle rest fungus (Puccinia) in Cereal belongs to, Cotton Gossypii, rhizoctonia (Rhizoctonia) in rice and lawn belongs to, smut (Ustilago) in Cereal and Caulis Sacchari sinensis belongs to, venturia inaequalis (Venturiainaequalis) (scab) in Fructus Mali pumilae, the compacted spore of length (Helminthosporium) in Cereal belongs to, wheat glume blotch bacterium (Septorianodorum) in Semen Tritici aestivi, Fructus Fragariae Ananssae, vegetable, Botrytis cinerea (Botrytiscinerea) (gray mold) in ornamental plant and vine, Semen arachidis hypogaeae tail spore (Cercosporaarachidicola) in Semen arachidis hypogaeae, eye spot bacterium (PseudocercosporellaHerpotrichoides) in Semen Tritici aestivi and Fructus Hordei Vulgaris, Pyricularia oryzae (Pyriculariaoryzae) in rice, phytophthora infestans (Phytophthorainfestans) in Rhizoma Solani tuber osi and Fructus Lycopersici esculenti, Fructus Vitis viniferae single shaft in vine is mould (Plasmoparaviticola), false downy mildew (Pseudoperonospora) in Flos lupuli (Flos Humuli Lupuli) and Fructus Cucumidis sativi belongs to, sclerotiniose (sclerotiose) in Brassica campestris L, rod method (Alternaria) in vegetable and fruit belongs to, spherical cavity bacterium (Mycosphaerella) in Fructus Musae belongs to and Neurospora (Fusarium) and wheel branch spore (Verticillium) genus。
Preferably, the bactericidal composition of the present invention can be used for preventing and treating on Cereal, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant gray mold, sclerotiniose, leaf spot, banded sclerotial blight, full rot, rust, anthrax, early blight, powdery mildew, scab, melasma, blight dis-ease, spot defoliation, stem wilt etc.。
The bactericidal composition of the present invention can be additionally used in preventing and treating fruit vegetables storing period disease。The fruit rot such as caused by following pathogen:
Aspergillus bacterium, for instance Aspergillus flavus；
Botrytis (Botrytis) strain, for instance Botrytis cinerea (Botrytiscinerea);
Penicillium (Penicillium) strain, for instance penicillium expansum (Penicilliumexpansum) and P.purpurogenum;
Sclerotinia (Sclerotinia) strain, for instance sclerotinite (Sclerotiniasclerotiorum);
Verticillium (Verticilium) strain, for instance black and white wheel branch spore (Verticiliumalboatrum).
The bactericidal composition of the present invention can be additionally used in preventing and treating kind and passes and soil-borne disease。The kind such as caused by following pathogen passes the rotten and wilting disease and seedling disease that pass with soil:
Alternaria belongs to plants, for instance the raw rod method (Alternariabrassicicola) of Caulis et Folium Brassicae campestris;
Aphanomyces (Aphanomyce) belongs to plants, for instance the mould (Aphanomyces of Kidney bean silk capsule
euteiches);
Ascochyta (Ascochyta) belongs to be planted, for instance Ascochytalentis;
Aspergillus, for instance Aspergillus flavus;
Cladosporium belongs to plants, for instance draft branch spore (Cladosporiumherbarum);
Cochliobolus belongs to plants, for instance standing grain cochliobolus;
(conidial form: Drechslera, Syn: long compacted spore bacterium);
Colletotrichum belongs to be planted, for instance hair core anthrax (Colletotrichumcoccodes);
Fusarium belongs to plants, for instance yellow sickle spore;
Gibberella belongs to plants, for instance beautiful another name for Sichuan Province multitude is red mould;
Shell ball spore belongs to (Macrophomina) genus kind, for instance Kidney bean shell ball spore (Macrophomina
phaseolina);
Little setting-out shell belongs to strain, for instance avenge rotten little setting-out shell;
Penicillium belongs to plants, for instance penicillium expansum;
Phaeosphaeria strain, for instance Phaeosphaerianodorum;
Phoma (Phoma) belongs to plants, for instance black embryo Phoma sp (Phomalingam);
Phomopsis (Phomopsis), for instance Semen sojae atricolor Phomopsis (Phomopsissojae);
Phytophthora belongs to plants, for instance Phytophthora cactorum (Phytophthoracactorum);
Nuclear cavity Pseudomonas belongs to be planted, for instance wheat and barley nuclear cavity bacteria (Pyrenophoragraminea);
Pyricularia Sacc. (Pyricularia) belongs to plants, for instance Magnaporthe grisea (Pyriculariaoryzae);
Pythium kind, for instance Pythium ultimum;
Rhizoctonia belongs to plants, for instance Rhizoctonia solani Kuhn;
Rhizopus (Rhizopus) belongs to plants, for instance Rhizopus oryzae (Rhizopusoryzae);
Bulbil belongs to (Sclerotium) genus kind, for instance Sclerotium rolfsii (Sclerotiumrolfsii);
Core coral Pseudomonas (Typhula) belongs to plants, for instance meat spore core coral bacterium (Typhulaincarnata);
Verticillium (Verticillium) belongs to plants, for instance verticillium dahliae (Verticilliumdahliae);
The bactericidal composition of the present invention, under preventing and treating phytopathogenic fungi desired concn, the compatibility having had with plant。Therefore, plant, plant propagation material and the plant organ grown subsequently, environment, habitat or storage areas can be applied to during the bactericidal composition of the present invention。
Term " plant propagation material " is interpreted as referring to all plant parts having fertility, for instance seed, and it can be used for breeding the latter, and vegetable matter such as cuttage bar or tuber (such as Rhizoma Solani tuber osi)。Therefore, plant part used herein includes plant propagation material。It is mentioned that such as seed (in the narrow sense), root, fruit, tuber, bulb, rhizome and plant part。The germination plant suppressed after germinateing from soil or after emerging and effective plant be also it can be mentioned that。Young plant can be undertaken all by dipping before transplantation or Local treatment is protected。
The plant organ grown subsequently is any part of the plant produced by plant propagation material such as seed。Plant part, plant organ and plant can also benefit from by active agent combinations or compositions are applied to plant propagation material protection plant avoid plant disease。Certain plants part and some plant organ grown subsequently can also regard plant propagation material as, himself can use (or process) with bactericidal composition；Thus the plant produced by treated plant part and treated plant organ, other plant part and other plant organ can also be benefited from avoids plant disease by bactericidal composition is applied to certain plants part and certain plants Organoprotective plant。
The environment of plant growing, habitat refer to the supporter making crops take root, growing, such as: soil, water etc., concrete raw material can use such as sand, Pumex, Vermiculitum, kieselguhr, agar, gelling material, polymer substance, asbestos, wood flour, bark etc.。Preferred soil。
According to the present invention, the process that plant is carried out by the bactericidal composition of the present invention is used to be made directly according to conventional treatment method, or realize by processing its local environment, habitat or storage space, described conventional treatment method such as immersion, spraying, evaporation, atomizing, broadcast sowing, smear；For plant propagation material or the plant organ that grows subsequently, especially seed, one or more layers coating also can be carried out。Type of service depends on desired purpose；Under any circumstance all it is ensured that the present composition as far as possible finely and is evenly distributed。Preferred application process is to be administered to aboveground vegetation part, especially foliage applying；Application times and use ratio and depend on biology and the weather conditions of existence of objective microbe。Active component can also reach in plant by root system via soil or water, and thus available liquid preparation waters location or this material is mixed in soil in solid form。The bactericidal composition of the present invention can also be administered on seed-grain in seed treatment, and thus tuber or corn can soak successively with the liquid preparation of every kind of active component, or wraps up with blended wet or dry preparation。
Effective ingredient mixes environment or the habitat at plant place, for instance mixing in the form of granules (soil application) in soil, effective ingredient enters inside plants also by root system。The method using medicament in soil, such as liquid preparation it is diluted in water or does not dilute the medium method in rice seedling bed of root or the seedling being directly applied to plant, the method being disseminated to by granule in the root of plant or the rice seedling bed of seedling has the method being sprayed in soil by powder, water dispersible granules etc. prior to seeding and mixing with soil entirety, it is sprayed in implantation hole, seed furrow after powder, water-dispersible grain dilution agent before sowing or before serike body, then carries out the method etc. sowed。
The bactericidal composition of the present invention is for protecting plant propagation material or plant organ such as seed, fruit, tuber or kernel or plant cutting not the infecting by plant disease grown subsequently; can before administration with this propagating materials of compositions-treated or the plant organ that grows subsequently, for instance dress seed prior to seeding。Also by soaking kernel in fluid composition or being coated kernel with solid composite, active component is applied on kernel (being coated)。When propagating materials being applied to that such as seed is broadcast in ditch in seeding process, it is possible to said composition is applied to application site。Processing method and the plant propagation material being processed as of plant propagation material are all further subject matters of the present invention by these。
In the preventing and treating of plant disease, bellkute and of pyrimethanil separately or the using by the spraying of seed, plant or soil or dusting being carried out before or after plant seeding or before or after plant emergence of the compositions of co-administered or bellkute and of pyrimethanil。The compositions of the present invention can be used before or after plant or plant propagation material are by infection process。
Product of the present invention can finished product preparation form provide, and namely in bactericidal composition, each material is blended, and the composition of bactericidal composition can also provide by unit dose form, directly directly mixes in bucket or in tank, be then diluted to required concentration before using。The frequency of administration of bactericidal composition of the present invention and amount of application, according to the mixing ratio of effective ingredient, meteorological condition, dosage form, Dressing date, application process, use place, prevent and kill off the difference of object noxious livings, objective crop etc. and variant:
-generally for leaf portion process active substance concentration be: 10-600g/ha, it is preferable that 30-500g/ha, more preferably 50-300g/ha；For using for dipping or instillation, described dosage even can also reduce, particularly when using inert base such as asbestos or ZHENZHUYANSHI；
-for seed treatment: 10-6000g active substance/100kg seed, it is preferable that 50-5000g active substance/100kg seed；
-for soil treatment: 10-6000g/ha, it is preferable that 50-3000g/ha；
-for fruit and vegerable impregnation process: 200-2000 times of liquid of dilute with water, leaching fruit one minute。
Above-mentioned dosage is only general exemplary dose, actual when using those skilled in the art according to practical situation and needs, rate of application can be adjusted especially in accordance with the character of pending plant or fruit and vegerable and the seriousness of pathogenic bacteria。
The bactericidal composition of the present invention can be used by common method, and amount of application changes with weather or crop condition, use optimum period be crop susceptible before or the susceptible initial stage。Its lasting period is generally relevant with the content in bactericidal composition, also relevant to extraneous factor。
The bactericidal composition of the present invention has the advantage that
1, the mechanism of action both bellkute and of pyrimethanil is complementary, it is possible to play better protection and therapeutical effect；
2, bellkute and Pyrimethanil compositional have obvious synergistic function, reduce every mu of using dosage of single medicament, thus both can reduce influence degree, significantly reducing again agricultural cost, the effect even preventing and treating some disease exceedes other antibacterial existing；
3, fungicidal spectrum is more extensive, to the disease caused by ascomycetes, basidiomycetes, Fungi Imperfecti, Phycomycetes in various crop, has good prevention effect。
Detailed description of the invention
Below with reference to embodiment, the invention will be further described:
Example of formulations:
Embodiment 12% bellkute+10% of pyrimethanil wettable powder
Bellkute 2%
Of pyrimethanil 10%
Sodium lauryl sulphate 10%
Sodium lignin sulfonate 5%
White carbon 10%
Kaolin complements to 100%
Reactive compound, various auxiliary agent and filler etc. are pressed the proportional components mixing of formula, after ultra-fine pulverizer disintegrating, namely obtains 2% bellkute+10% of pyrimethanil wettable powder。
Embodiment 250% bellkute+1% of pyrimethanil wettable powder
Bellkute 50%
Of pyrimethanil 1%
Calcium dodecyl benzene sulfonate 1%
Sodium lignin sulfonate 2%
Sucrose complements to 100%
Reactive compound, various auxiliary agent and filler etc. are pressed the proportional components mixing of formula, after ultra-fine pulverizer disintegrating, namely obtains 50% bellkute+1% of pyrimethanil wettable powder。
Embodiment 31% bellkute+4% of pyrimethanil cream
Bellkute 1%
Of pyrimethanil 4%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
Dimethyl sulfoxide complements to 100%
Mentioned component is proportionally prepared, is uniformly mixing to obtain homogeneous phase。
Embodiment 450% bellkute+5% of pyrimethanil wettable powder
Bellkute 50%
Of pyrimethanil 5%
Sodium lauryl sulphate 10%
Sodium lignin sulfonate 5%
White carbon 10%
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder。
Embodiment 52% bellkute+50% pyrimethanil water dispersible granule
Bellkute 2%
Of pyrimethanil 50%
MODIFIED LIGNOSULPHONATE 5%
Sodium lauryl sulphate 5%
Carbamide 5%
Kaolin complements to 100%
By bellkute, of pyrimethanil, dispersant, wetting agent, disintegrating agent and filler in the ratio mix homogeneously of formula, become wettable powder through comminution by gas stream；Add a certain amount of water mixing extruding and make material。2% bellkute+50% pyrimethanil water dispersible granule is obtained after drying screening。
Embodiment 60.5% bellkute+50% of pyrimethanil suspension emulsion
Oil phase:
Bellkute 0.5%
Methyl oleate 10%
Ethoxylated castor oil 5%
Aqueous phase:
Of pyrimethanil 50%
The sodium salt 1% of the LOMAR PWA EINECS 246-676-2-formaldehyde condensation products of sulfonation
Water complements to 100%
Bellkute is dissolved in methyl oleate, adds ethoxylated castor oil and obtain oil phase；The suspending agent of aqueous phase of pyrimethanil will be obtained after ground to of pyrimethanil, the sodium salt of LOMAR PWA EINECS 246-676-2-formaldehyde condensation products of sulfonation, water and/or high speed shear according to formula；Under agitation oil phase is added aqueous phase and obtain suspension emulsion。
Embodiment 710% bellkute+10% of pyrimethanil wettable powder
Bellkute 10%
Of pyrimethanil 10%
Sodium lignin sulfonate 1%
Sodium lauryl sulfate 2%
The silicic acid 1% of high degree of dispersion
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder。
Embodiment 840% bellkute+50% of pyrimethanil coated granule
Bellkute 40%
Of pyrimethanil 50%
Polyethylene Glycol 3%
The silicic acid 1% of high degree of dispersion
Calcium carbonate complements to 100%
In a mixer, by levigate Fungicidal active compound even spread to by the carrier of Polyethylene Glycol moistening。Dust-free coated granules agent can be obtained in this way。
Embodiment 950% bellkute+10% of pyrimethanil wettable powder
Bellkute 50%
Of pyrimethanil 10%
Sodium lauryl sulphate 1%
Sodium lignin sulfonate 1%
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder。
Embodiment 1020% bellkute+60% of pyrimethanil granule
Bellkute 20%
Of pyrimethanil 60%
Sodium lignin sulfonate 4%
Carboxymethyl cellulose 2%
Kaolin complements to 100%
Reactive compound mixed with auxiliary agent and grinds, with water-wet, pelletize, then drying in the air stream。
Embodiment 1150% bellkute+2% of pyrimethanil seed coat agent
Bellkute 50%
Of pyrimethanil 2%
Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium 10%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Bentonite 1%
Glycerol 5%
PVP-K301%
Water complements to 100%
Seed coat agent is obtained after above-mentioned each component is mixed in proportion ground and/or high speed shear。
Embodiment 125% bellkute+50% of pyrimethanil microcapsule suspension-suspendinagent agent
ATLOXTM49134%
Citric acid 0.05%
Catalyst 0.1%
Water 8%
Of pyrimethanil 50%
PAPITM1.35%
SOLVESSOTM2005%
ATLOXTM491316%
Dispersant LFH0.3%
Defoamer 0.16%
Carbamide 4.4%
Bellkute 5%
Water complements to 100%
By PAPITM, bellkute, SOLVESSOTM200 oil phases formed add containing ATLOXTMIn the aqueous solution of 4913, form emulsion。Then heating insulation are 50oCatalyst reaction is added 2 hours under C。The microcapsule of bellkute is obtained after cooling。
ATLOXTM4913, dispersant LFH, defoamer, carbamide, of pyrimethanil and water are mixed in proportion, ground and/or obtain suspending agent after high speed shear。
The bellkute microcapsule obtained is added in the suspending agent of of pyrimethanil, be uniformly mixing to obtain 5% bellkute+50% of pyrimethanil microcapsule suspension-suspendinagent agent。
Embodiment 131% bellkute+50% of pyrimethanil suspension emulsion
Bellkute 1%
Of pyrimethanil 50%
SOLVESSOTM20010%
Ethoxylated castor oil 4%
Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Bentonite 1%
Glycerol 5%
Water complements to 100%
Bellkute is dissolved in SOLVESSOTMIn 200, add ethoxylated castor oil, obtain the oil phase of bellkute；
Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium, MODIFIED LIGNOSULPHONATE, of pyrimethanil and water are mixed in proportion, ground and/or after high speed shear, obtain the suspending agent of of pyrimethanil。
Oil phase containing bellkute is joined the aqueous suspension agent of pyrimethanil-contain bactericide, obtains suspension emulsion。
Embodiment 1450% bellkute+0.5% pyrimethanil water dispersible granule
Bellkute 50%
Of pyrimethanil 0.5%
MODIFIED LIGNOSULPHONATE 5%
Sodium lauryl sulphate 5%
Carbamide 5%
Kaolin complements to 100%
By bellkute, RP-26019, dispersant, wetting agent, disintegrating agent and filler in the ratio mix homogeneously of formula, become wettable powder through comminution by gas stream；Add a certain amount of water mixing extruding and make material。50% bellkute+0.5% iprodione water dispersible granules is obtained after drying screening。
Embodiment 1520% bellkute+30% pyrimethanil water dispersible granule
Bellkute 20%
Of pyrimethanil 30%
MODIFIED LIGNOSULPHONATE 5%
Sodium lauryl sulphate 5%
Carbamide 5%
Kaolin complements to 100%
By bellkute, of pyrimethanil, dispersant, wetting agent, disintegrating agent and filler in the ratio mix homogeneously of formula, become wettable powder through comminution by gas stream；Add a certain amount of water mixing extruding and make material。20% bellkute+30% pyrimethanil water dispersible granule is obtained after drying screening。
Embodiment 165% bellkute+5% of pyrimethanil wettable powder
Bellkute 5%
Of pyrimethanil 5%
Sodium lauryl sulphate 1%
Sodium lignin sulfonate 1%
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder。
Embodiment 1750% bellkute+50% of pyrimethanil conjugate
Bellkute 50%
Of pyrimethanil 50%
Proportioning in above example is weight per distribution ratio。
Biological test example
The combination of active principles of difference pesticide is made pesticide, is develop and develop novel pesticide at present and prevent and treat a kind of effective and quick way of agriculturally resistance pathogenic bacteria。After the pestsides synthesis of different cultivars, typically exhibit three kinds of type of action: summation action, potentiation and antagonism。But it is specially which kind of effect, it is impossible to prediction, only just be can know that by great many of experiments。The good formula of compound synergic, owing to significantly improving actual prevention effect, what reduce pesticide makes consumption, thus greatly having delayed the drug-fast generation speed of pathogenic bacteria, is the important means of integrated control disease。
The combination of bellkute and of pyrimethanil is carried out effect analysis by the abundant method of Sun Yun, Colby formula by the present invention, it has been found that in certain ratio range, and obtained bactericidal composition has gain effect, and is not only the simple addition of two kinds of medicaments。
Toxicity Determination
Test 1: the toxicity test to graw mold of tomato
Adopt spore germination test method(s)。Choosing the tomato seedling that growing way is consistent, each process selects 3 basins for examination tomato seedling, sprays under 50PSI pressure with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 Concentraton gradient。After chemicals treatment, 24h connects bacterium, the graw mold of tomato leaf picking up from field is uniformly shaken off conidium above tomato seedling and inoculates, and then tomato seedling is put in greenhouse and cultivates。Investigate disease index according to the morbidity grade scale Herb of graw mold of tomato after 7d, and calculate prevention effect, then calculate concentration EC in suppressing with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun。
Computational methods:
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
The percentage composition of B in the percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI) * 100
CTC < 80 is antagonism, and 80≤CTC≤120 are summation action, and CTC > 120 is potentiation,
Table 1: the virulence test result to graw mold of tomato
As known from Table 1, when the combination preventing and treating graw mold of tomato of bellkute and of pyrimethanil is in the scope of proportioning 100:1-1:100, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at the mixture within the scope of this。
Test 2: the toxicity test of powdery mildew of cucumber
Adopting spore germination test method(s): choose the cucumber seedling that growing way is consistent, each process selects 3 basins for examination leaf Seedling, sprays under 50PSI pressure with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 Concentraton gradient。After chemicals treatment, 24h connects bacterium, the powdery mildew of cucumber leaf picking up from field is uniformly shaken off conidium above cucumber seedling and inoculates, and is then put into by cucumber seedling in greenhouse and cultivates。Investigate disease index according to the morbidity grade scale Herb of powdery mildew of cucumber after 7d, and calculate prevention effect, then calculate concentration EC in suppressing with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun。Table 2: the virulence test result to powdery mildew of cucumber
As known from Table 2, the combination of bellkute and of pyrimethanil prevent and treat powdery mildew of cucumber in the scope of proportioning 100:1-1:100 time, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at mixture within the scope of this。
Test 3: the toxicity test of sclerotinia rot of colza
Adopting spore germination test method(s): choose the Brassica campestris L seedling that growing way is consistent, each process selects 3 basins for examination seedling, sprays under 50PSI pressure with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 Concentraton gradient。After chemicals treatment, 24h connects bacterium, the sclerotinia rot of colza leaf picking up from field is uniformly shaken off conidium above Brassica campestris L seedling and inoculates, and is then put into by Brassica campestris L seedling in greenhouse and cultivates。Investigate disease index according to the morbidity grade scale Herb of sclerotinia rot of colza after 7d, and calculate prevention effect, then calculate concentration EC in suppressing with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun。
Table 3: the virulence test result to sclerotinia rot of colza
As known from Table 3, when the combination preventing and treating sclerotinia rot of colza of bellkute and of pyrimethanil is in the scope of proportioning 100:1-1:100, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at the mixture within the scope of this。
Test 4: the toxicity test of leaf spot of peanut
Adopting spore germination test method(s): choose the Semen arachidis hypogaeae Seedling that growing way is consistent, each process selects 3 basins for examination leaf Seedling, sprays under 50PSI pressure with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 Concentraton gradient。After chemicals treatment, 24h connects bacterium, the leaf spot of peanut leaf picking up from field is uniformly shaken off conidium above Semen arachidis hypogaeae Seedling and inoculates, and is then put into by Semen arachidis hypogaeae Seedling in greenhouse and cultivates。Investigate disease index according to the morbidity grade scale Herb of leaf spot of peanut after 7d, and calculate prevention effect, then calculate concentration EC in suppressing with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun。
Table 4: the virulence test result to leaf spot of peanut
As known from Table 4, the combination of bellkute and of pyrimethanil prevent and treat leaf spot of peanut in the scope of proportioning 100:1-1:100 time, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at mixture within the scope of this。
Test 5: the toxicity test of Penicillium italicum
Adopting and suppress mycelial growth rate method: experiment target is designated as Penicillium italicum, test adopts co-toxicity coefficient method to evaluate the co-toxicity effect of complex preparation。
Bellkute and of pyrimethanil are used acetone solution respectively, the medicinal liquid being configured to series concentration is diluted again with the tween 80 aqueous solution of 0.1%, superclean bench is drawn the 6mL conical flask to sterilizing respectively, add potato dextrose agar (PDA) 54mL of about 50 DEG C, pour the plate of 4 diameter 9cm after shaking up into, make the toxic culture medium of 4 respective concentration；Toxic culture medium is made by the same method bellkute by different ratio and of pyrimethanil series concentration compound solution。By cultivating the Penicillium italicum bacterium of 2 days, break into truffle with the card punch of diameter 5mm at colony edge, with Inoculating needle, truffle is moved to the toxic PDA culture medium central authorities being configured in advance, is subsequently placed in 25 DEG C of incubators and cultivates, often process and repeat 4 times。After 3 days, adopt decussation method slide calliper rule to measure each process colony diameter cm, obtain correction inhibition percentage。Each bacterium colony decussation surveys two diameters, represents bacterium colony size with its average。Then colony growth suppression ratio is obtained by following formula:
Then concentration EC in suppressing is calculated with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun。
Table 5: the virulence test result to Penicillium italicum
As known from Table 5, when the combination preventing and treating Penicillium italicum of bellkute and of pyrimethanil is in the scope of proportioning 100:1-1:100, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at the mixture within the scope of this。
Biological test example
When the effect of active compound combinations exceedes the summation of the effect when each reactive compound is individually used, there is synergistic function。The predictive role of the particular combination of two kinds of reactive compounds can use so-called " Colby formula " (referring to S.R.Colby, " CalculatingSynergisticandAntagonisticResponsesofHerbicid eCombinations ", Weeds1967,15,20-22) calculated as below: if
X is the activity when using the reactive compound A that consumption is mg/ha or concentration is mppm；
Y is the activity when using the reactive compound B that consumption is ng/ha or concentration is nppm, is expressed as accounting for the percentage rate of untreated control；
E is the activity when using the reactive compound A and B that consumption is m and ng/ha or concentration is m and nppm,
So
If the activity of actual observation (O) is more than expection activity (E), then said composition is super add and, namely there is potentiation。
Following biological test example is in order to illustrate the present invention。But, the present invention is not limited to these embodiments。
Test 1 powdery mildew of cucumber test
Solvent: the N-methyl-pyrrolidon of 7 parts of weight
Emulsifying agent: the alkylaryl polyglycol ether of 2 parts of weight
Suitable in order to prepare, the combination of 1 part of reactive compound by weight or reactive compound is mixed with solvent and the emulsifying agent of above-mentioned amount, and is diluted with water to desired concn。
In order to measure activity, the seedling active agent preparations of described using dosage is sprayed。The spore of seedling cucumber powdery mildew fungus is dusted after becoming dry by liquid layer to be painted。Then test plant is placed in temperature about 20OTo promote the growth of white lead scab in the greenhouse of C, air humidity about 80%。Carrying out effect assessment after inoculating 7 days, 0% to refer to effect suitable with what compare, and 100% refers to and do not observe and infect。Reactive compound, using dosage and result of the test are listed in the following table:
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates。
Test 2 ash arrhizus bacterias test (Fructus Fragariae Ananssae)
Solvent: the N-methyl-pyrrolidon of 7 parts of weight
Emulsifying agent: the alkylaryl polyglycol ether of 2 parts of weight
In order to prepare suitable reactive compound, the combination of 1 part of reactive compound by weight or reactive compound is mixed with solvent and the emulsifying agent of above-mentioned amount, and is diluted with water to desired concn。
In order to test therapeutical active, seedling Botrytis cinerea bacterium spore is dusted。After inoculating 48 hours, the Strawberry seedlings active agent preparations of described using dosage is sprayed。Then test plant is placed in temperature about 20OTo promote the growth of grey mold scab in the greenhouse of C, air humidity about 80%。Carrying out effect assessment after inoculating 7 days, 0% to refer to effect suitable with what compare, and 100% refers to and do not observe and infect。Reactive compound, using dosage and result of the test are listed in the following table:
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates。
Test 3 sclerotiniose test (Brassica campestris L)/seed treatment
Reactive compound is used with dry seeds seed dressing。Said preparation can be prepared by being mixed with levigate mineral by the reactive compound of independent reactive compound or combination, and obtaining can at the equally distributed finely powdered mixt of the surface of the seed。
During seed seed dressing, seed and seed seed dressing shake 3 minutes in the glass flask closed will be infected。Semen Brassicae campestris is seeded in standard soil 1 centimetre deep, in temperature about 18OC, the warm indoor cultivation of air humidity about 95%, every 15 h light day by day of seeds cultivation case。
After sowing 3 weeks, evaluating the disease of Brassica campestris L seedling, 0% to refer to effect suitable with what compare, and 100% refers to and do not observe and infect。Reactive compound, using dosage and result of the test are listed in the following table:
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates。
Test 4: the antiseptic effect to storage Citrus
Process and pluck Fructus Citri tangerinae fruit collection period in Citrus, reject sick fruit and wound fruit, with processing medicinal liquid leaching fruit 1min。Comparison clear water dipping。Drying after each process leaching fruit, load surrounding next day and be lined with the plastic box of newspaper, normal temperature laboratory is preserved。Process latter 45 days and check decayed fruit number and reject decayed fruit, weigh up fruit weight simultaneously。And calculate preventive effect。
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates。
Claims (14)
1. a bactericidal composition, it is characterised in that: active component is made up of bellkute and of pyrimethanil, and the weight ratio of described bellkute and of pyrimethanil is 100:1-1:100。
2. bactericidal composition according to claim 1, it is characterised in that: the weight ratio of described bellkute and of pyrimethanil is 50:1-1:50。
3. bactericidal composition according to claim 1, it is characterised in that: the weight ratio of described bellkute and of pyrimethanil is 25:1-1:25。
4. bactericidal composition according to claim 1, it is characterised in that: the weight ratio of described bellkute and of pyrimethanil is 10:1-1:10。
5. bactericidal composition according to claim 1, it is characterised in that: comprise the active component described in claim 1, filler and/or surfactant。
6. bactericidal composition according to claim 1, it is characterised in that: the content of bellkute and of pyrimethanil active component accounts for the 5%-90% of compositions。
7. bactericidal composition according to claim 1, it is characterised in that: the content of bellkute and of pyrimethanil active component accounts for the 10%-80% of compositions。
8. bactericidal composition according to claim 1, it is characterised in that: the content of bellkute and of pyrimethanil active component accounts for the 20%-60% of compositions。
9. bactericidal composition according to claim 1, it is characterized in that, the dosage form of described compositions is wettable powder, cream, suspending agent, suspension emulsion, microcapsule, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foam, unguentum, aerosol, ultra-low volume spray liquid。
10. the bactericidal composition described in claim 1 is for preventing and treating the purposes of fungus on Cereal, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant。
11. the method preventing and kill off plant disease, the bactericidal composition described in claim 1 is applied to plant, plant propagation material and the organ grown subsequently, environment。
12. method according to claim 11, it is characterised in that: the propagating materials needing plant to be protected is processed with the bactericidal composition described in claim 1。
13. method according to claim 11, it is characterised in that: the bellkute of claim 1 and of pyrimethanil compound are used simultaneously or are used respectively or sequential application。
14. the bactericidal composition described in claim 1 is for preventing and treating the purposes of fruit vegetables storing period fungal disease。
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| CN101916802A (en) * | 2005-09-27 | 2010-12-15 | 飞利浦拉米尔德斯照明设备有限责任公司 | III-V luminescent device |
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