CN110384109A - A kind of bactericidal composition - Google Patents

A kind of bactericidal composition Download PDF

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Publication number
CN110384109A
CN110384109A CN201810354477.8A CN201810354477A CN110384109A CN 110384109 A CN110384109 A CN 110384109A CN 201810354477 A CN201810354477 A CN 201810354477A CN 110384109 A CN110384109 A CN 110384109A
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plant
bactericidal composition
bacterium
calcium sulfate
seed
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罗昌炎
詹姆斯.T.布里斯托
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Jiangsu Rotam Chemical Co Ltd
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Jiangsu Rotam Chemical Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a kind of bactericidal compositions, the bactericidal composition for more particularly, to protecting plant or seed to invade from pathogenic epiphyte.The present invention provides a kind of bactericidal composition, and the bactericidal composition contains active constituent biphenyl pyrazine bacterium amine and copper calcium sulfate, and wherein biphenyl pyrazine bacterium amine and the weight proportion of copper calcium sulfate are 1:10-1:400.The invention further relates to the bactericidal composition be used to control cereal, bean, fruit tree and fruits, greengrocery, root vegetables, forage grass class, turfgrass or turf, spice crop, on flower plant fungi and bacterium purposes.

Description

A kind of bactericidal composition
Technical field
The present invention relates to a kind of bactericidal compositions, and plant or seed is more particularly, to protected to invade from pathogenic epiphyte Bactericidal composition.
Background technique
At present for being agriculturally also easy to produce the disease control of resistance, the pesticide species for using the mechanism of action different carry out mixture It is optimal selection, if proportion is rationally, can produce significant synergistic effect, field control effect is made to be substantially better than each list The effect of agent.Fungicide containing single active ingredient is usually present certain defect, continuous several times in agricultural disease prevention and treatment Using not only making pathogen be also easy to produce drug resistance, and easily cause the pollution to food and environment, by fungicidal activity at point it Between rational mixture can overcome disadvantage mentioned above.The synergistic effect that reasonable compounding generates effective component, can be improved preventive effect, subtracts Few effective ingredient dosage, save the cost delays the generation of the drug resistance of pathogen, and then can reduce or even avoid pesticide to food The pollution of object and environment.
About pesticide activity, especially to crop protection, the key problem for the research carried out in the technical field first is that Improve the performance in terms of performance, especially bioactivity and the performance in terms of keeping this activity within a certain period of time.
Biphenyl pyrazine bacterium amine (Pyraziflumid), CAS:942515-63-1；Molecular structural formula are as follows:
Biphenyl pyrazine bacterium amine is the mould inhibitor class fungicide of succinic acid dehydrogenation of Nihon Nihyaku Co., Ltd's exploitation.Biphenyl pyrazine bacterium Amine is by known in CN101341136A.
Due to now constantly improve to the environmental requirement of fungicide and economic requirement, such as to activity profile, toxicity, selection Property, rate of application, residual and it is advantageous preparation feasibility requirement, additionally since there may be ask in terms of such as drug resistance Topic.Therefore, exploitation is in some aspects lasting task better than the new fungicide of existing fungicide.
Summary of the invention
The object of the present invention is to provide a kind of bactericidal compositions, apply in the case where reduced reactive compound applies total amount with regard to reducing With rate and for improving known compound biphenyl pyrazine bacterium amine and the activity profile of copper calcium sulfate, the present invention is reducing reactive compound It applies under total amount, having to pathogenic epiphyte improves active (synergy).
We have found that simultaneously, i.e., jointly or separately applying biphenyl pyrazine bacterium amine and copper calcium sulfate, or successively apply biphenyl Pyrazine bacterium amine and copper calcium sulfate preferably to prevent and treat harmful fungoid or bacterium than each compound is administered alone.
The present invention provides a kind of bactericidal composition, the composition is by carrying out two for biphenyl pyrazine bacterium amine and copper calcium sulfate Member compounding, so that obtained composition has gain effect in control efficiency, and has expanded fungicidal spectrum, it is more to play a medicine Effect effectively slows down or germ is avoided to develop drug resistance.Astoundingly, the bactericidal activity of bactericidal composition of the invention It is more considerably higher than the active adduction of each reactive compound.In other words, there are unpredictable, necessary being collaboration effects It answers, rather than just active supplement.
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright It is aobvious.But the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.
A kind of bactericidal composition of the present invention is to adopt the following technical solutions to achieve:
A kind of bactericidal composition, it is characterised in that: containing active constituent biphenyl pyrazine bacterium amine and copper calcium sulfate, wherein biphenyl pyrazine Bacterium amine and the weight proportion of copper calcium sulfate are 1:10-1:400, preferably 1:20-1:350, preferably 1:30-1:300, more preferable 1: 30-1:250；More preferably 1:30-1:200, more preferably 1:40-1:200, more preferably 1:50-1:200, further preferably For 1:50-1:180, further preferably 1:60-1:160, further preferably 1:70-1:150, further preferably 1:80- 1:150。
Biphenyl pyrazine bacterium amine in the present invention and the weight proportion of copper calcium sulfate for example can also be 1:10,1:15,1:20, 1:25,1:30,1:35,1:40,1:45,1:50,1:55,1:60,1:65,1:70,1:75,1:80,1:85,1:90,1:95,1: 100,1:105,1:110,1:115,1:120,1:125,1:130,1:135,1:140,1:145,1:150,1:155,1:160, 1:165,1:170,1:175,1:180,1:185,1:190,1:195,1:200,1:205,1:210,1:220,1:230,1: 240,1:250,1:260,1:270,1:280,1:290,1:300,1:310,1:320,1:330,1:340,1:350,1:360, 1:370,1:380,1:390,1:400。
The bactericidal composition, it is characterised in that: the biphenyl pyrazine bacterium amine and copper calcium sulfate quality sum account for described The 5%-90% of Bactericidal composite amount of substance, more preferable 10%-80%, more preferable 20%-75%, more preferable 20%-70%.
In bactericidal composition of the invention, the content of the biphenyl pyrazine bacterium amine and copper calcium sulfate accounts for the bactericidal composition By weight can also be, for example: 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%, 35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%, 54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%, 73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%。
A method of bactericidal composition is acted on pathogenic bacteria and/or its environment, Huo Zhezhi by prevention and treatment plant pathogen In object, plant parts, seed, soil, region, material or space.
A method of prevention and treatment plant pathogen, biphenyl pyrazine bacterium amine and copper calcium sulfate are administered simultaneously or applied respectively, Or sequential application.
A kind of bactericidal composition includes biphenyl pyrazine bacterium amine and copper calcium sulfate, filler and/or surfactant.
A kind of bactericidal composition can be configured to any dosage form agriculturally allowed.The bactericidal composition, dosage form are Aqueous suspension agent, seed treatment suspending agent, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspending agent, granule, missible oil, Microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids, thermal fog, dry suspending agent, oil-suspending agent.
The bactericidal composition for prevent and treat cereal, bean, fruit tree and fruits, greengrocery, root vegetables, Forage grass class, turfgrass or turf, spice crop, flowers etc..
The bactericidal composition is for protecting plant, plant propagation material and the plant organ then grown from fungi With the purposes of bacteria attack.The plant propagation material is seedling, rhizome, garden seedling, cutting or seed.
The bactericidal composition is for handling seed to protect seed from the purposes of fungi and bacteria attack.
The bactericidal composition is used to handle the soil of plant growth to protect the plants from the fungi in soil and thin The purposes of bacterium invasion.
The place prevention and treatment soil that the bactericidal composition is prevented and treated needed for being applied to causes a disease or the use of saprophytic fungi and bacterium On the way.
The bactericidal composition is used to prevent and treat the purposes of fruit vegetables storing period disease.
Bactericidal composition of the invention has very strong activity to various phytopathogens, and can draw to by phytopathogen The prevention and treatment of the plant disease risen play very strong preventive effect.Bactericidal composition of the invention to such as Plasmodiophoromycetes, Oomycete, Chytridiomycetes connect with wide scopes plant pathogenic fungis such as Gammaproteobacteria, Ascomycetes, Basidiomycetes, deuteromycetes with pole Good activity.
Bactericidal composition of the invention also has extraordinary bactericidal performance, and it is thin to can be used for prevention and treatment plant pathogenic Bacterium.Such as pseudomonadaceae, Rhizobiaceae, enterobacteriaceae, rod Cordycepps and Streptomycetaceae.
Bactericidal composition of the invention can be used as foliage fungicide in crop protection, can also be used as fungicide for mixing Plant and be used as soil fungicides.
Bactericidal composition of the invention also can be used for preventing and treating the disease of fruit vegetables storing period.
Bactericidal composition of the invention is suitable for preventing and treating following phytopathogen:
Oomycete, including Phytophthora (Phytophthora), such as phytophthora infestans (Phytophthora infestans), Phytophthora sojae kaufmann&gerdemann (Phytophthora megasperma), foot rot of citrus bacterium (Phytophthora parasitica), The disease of camphor tree phytophthora (Phytophthora cinnamomi) and phytophthora capsici (Phytophthora capsici)；Grass The rotten mould withered disease for belonging to (Pythium) such as level ground grass Pythium aphanidermatu (Pythium aphanidermatum)；And frost Mould section (Peronosporaceae) disease such as Plasmopara viticola (Plasmoparaviticola)；Peronospora (Peronospora) (including Peronosporatabacina (Peronospora tabacina) and Peronospora parasitic bacterium (Peronospora parasitica))；Pseudoperonospora (Pseudoperonospora category) germ (including bacterium of downy mildew of cucumber (Pseudoperonospora cubensis) and disk obstruct mould germ (Bremialactucae)；Pythium (Pythium) example Such as scraping and printing unit (Pythium aphanidermatum), black leg of beet bacterium (Pythium ultium)；Plasmopara (Plasmopara) such as Plasmopara viticola (Plasmopara viticola).
Ascomycetes, including Alternaria (Alternaria) disease such as tomato early blight bacterium (Alternaria ) and black spot of cabbage bacterium (Alternaria brassicae) solani；Ball seat Pseudomonas (Guignardia) disease such as grape Black rot (Guignardia bidwelli)；Venturia (Venturia) disease such as apple black star bacteria (Venturia inaequalis)；Septoria (Septoria) disease such as glume blight bacterium (Septoria nodorum) and leaf spoting bacteria (Septoriatritici)；Erysiphe (Erysiphe) such as wheat powdery mildew (Erysiphegraminis) and radish Powdery Mildew (Erysiphepolygoni)), grape powdery mildew (Uncinulanecatur), cucumber powdery mildew's pathogen (Sphaerotheca fuligena) and apple mildew bacterium (Podosphaera leucotricha)；Wheat Phyllostachys pubescens (Pseudocercosporella herpotrichoides) species；Grey mold Pseudomonas (Botrytis) species disease such as strawberry Ash arrhizus bacteria (Botrytiscinerea), Monilinia fructicola (Monilinia fructicola) disease；Pyrenomycetes category (Sclerotinia) species disease such as Sclerotinia sclerotiorum (Sclerotinia sclerotiorum)；Magnaporthe grisea category (Pyricularia oryzae Cav.) such as Pyricularia oryzae (Magnaporthe grisea)；Compacted shape Pseudomonas (Helminthosporium) species disease such as Exserohilum turcicum (Helminthosporium Tritici Repentis), reticulate pattern germ (Pyrenophorateres) species；Colletotrichum (Colletotrichum category) such as fine strain of millet anthrax Germ (Colletotrichum graminicola) and watermelon anthrax bacteria (Colletotrichum orbiculare)；Standing grain Top capsule shell (Gaeumannomyces gramims) such as gaeumannomyces graminis (Gaeumannomyces graminis)；Cross hair Sphaerotheca (Podosphaera)；Chain sclerotinia sclerotiorum belong (Monilinia)；Uncinula (Uncinula)；Mycosphaerella (Mycosphaerella)；Leptosphaeria (Leptosphaeria);Podosphaera (Blumeria);Sclerotinia category (Sclerotinia);Gaeumannomyce category (Gaeumannomyces);Mycotoruloides (Monilinia).
Basidiomycetes, including the rest fungus disease as caused by Rust (Puccinia) (such as Puccinia recondita (Pucciniarecondita), strip rust bacteria (Puccinia striiformis), leaf rust (Pucciniahordei), puccinia graminis Bacterium (Puccinia graminis) and handle rest fungus (Puccinia arachidis)), coffee rest fungus (Hemileia Vastatrix), camel spore rest fungus category (Hemileia), phakopsora pachyrhizi sydow (Phakopsora pachyrhizi)；Ustilago (Listilaginalcs).
Deuteromycetes, including Rhizoctonia (Rhizoctonia category) species (such as Rhizoctonia solani Kuhn (Rhizoctonia Solani), redness of the skin or complexion hyphal cluster germ (Rhizoctonia oryzae), Oryza sheath blight fungus (Rhizoctonia solani);Sickle Knife Pseudomonas (Fusarium) disease, such as Fusarium graminearum (Fusarium graminearum), beads Fusariumsp (Fusarium ), moniliforme Fusarium oxysporum (Fusarium oxysporum), fusarium moniliforme (Fusarium ), proliferatum Fusaium solani (Fusarium solani)；Verticillium dahliae (Verticillium dahliae)； Sclerotiumrolfsii (Sclerotium rolfsii)；Moire bacterium (Rynchosporium secalis);Black puckery germ (Cercosporidium personatum), alternaria (Cercospora arachidicola) and brown patch germ (Cercospora beticola);Silver dollar pinta bacterium (Rutstroemia floccosum)；Botrytis (Botrytis)； Pyricularia Sacc. (Pyricularia)；Helminthosporium (Helminthosporium)；Fusarium (Fusarium)；Septoria (Septoria)；Cercospora (Cercospora)；Alternaria (Alternaria) such as Alternaria mali (Alternaria mali), tomato early blight bacterium (Alternaria solani)；Pyricularia Sacc. (Pyricularia);False small tail Spore category (Pseudocercospora) such as sugar beet leaf spot bacteria (Cercospora beticola);Blastocladia bacterium such as peach is black Star germ (Cladosporium carpophilum)；Phoma bacterium such as lucid asparagus Phoma asparagi Sacc (Phomasparagi)；Shell Needle spore category bacterium such as wheat crust blight bacterium (Septoria nodorum);Colletotrichum bacterium such as Colletotrichumlagenarium (Colletotrichum largenirium)；Pyricularia category bacterium such as wilt (Pyricularia oryzae), Botrytis bacterium such as greengrocery ash arrhizus bacteria (Botrytis cinerea)；Apple bivalve (Diplocarpon mali Harada Et Sawamura) and apple Marssonina (Marssonina coronaria) caused by apple marssonina leaf spot.
Bactericidal composition of the invention is particularly suitable for preventing and treating following phytopathogen: botrytis category, Sclerotinia, clump stalk Spore category, Alternaria, Erysiphe, Rhizoctonia, Fusarium.
The suitable crop of bactericidal composition of the invention specifically includes that
Cereal (such as rice, barley, wheat, rye, oat, corn, jowar plant), bean (such as soybean, red small The plants such as beans, semen viciae fabae, pea, peanut), fruit tree and fruits (such as apple tree, citrus fruit trees, pear tree, vine, peach, plum Tree or Japanese plum, cherry tree, walnut tree, apricot, Banana tree, strawberry etc. and their fruit), greengrocery (such as wild cabbage, western red The plants such as persimmon, spinach, cauliflower, lettuce, onion, shallot, pumpkin, cucumber, watermelon, muskmelon), root vegetables (such as carrot, potato, The plants such as sweet potato, japanese radish, turnip), insutrial crop or crop (such as cotton, hemp, paper mulberry, oil for industrial processes Dish, beet, hops, sugarcane, sugar beet, Chinese olive tree, rubber tree, coffee tree, tobacco, tea tree etc.), forage grass class (such as orchard Turfgrass, jowar, timothy grass, clover etc.), turfgrass or turf, spice crop etc. (such as lavender, be confused folded perfume, thyme, Parsley, pepper, ginger etc.), flowers (such as chrysanthemum, rose, orchid) etc..
The bactericidal composition of the invention preferably prevention such as following plant disease and control:
The brown patch germ (Cercospora beticola) of " beet ", black root germ (Aphanomyces cochlloides), Pine root fungus (Thanatephorus cucumeris), leaf rot bacterium (Thanatephorus cucumeris), rhizoctonia solani (Pythium ultium);
Brown patch germ (Mycosphaerella arachidis), the alternaria (Mycosphaerella of " peanut " Berkeleyi), pine root fungus (Thanatephorus cucumeris).
Powdery Mildew (Sphaerotheca fuliginea), the Pseudoperonospora cubensis (Pseudoperonospora of " cucumber " Cubensis), didymella bryoniae (Mycosphaerella melonis), dead arm bacterium (Fusarium oxysporum), sclerotium Germ (Sclerotinia sclerotiorum), ash arrhizus bacteria (Botrytis cinerea), anthracnose (Colletotrichum orbiculare), black star germ (Cladosporium cucumerinum), brown patch germ (Corynespora cassicola), seedling blight bacterium (Pythium debaryanam, Rhizoctonia solani Kuhn), bacterial leaf spot (Pseudomonas syringae pv.Lecrymans)；
Ash arrhizus bacteria (Botrytis cinerea), the Fulvia fulva Ciferri (Cladosporium fulvum), late disease bacteria of " tomato " (Phytophthora infestans), pine root fungus (Thanatephorus cucumeris), dry rot germ (Sclerotinia Sclerotiorum)；
The ash arrhizus bacteria (Botrytis cinerea) of " eggplant ", bacterial blight bacterium (Corynespora melongenae), white powder Germ (Erysiphe cichoracearum), suede bacterial plaque germ (Mycovellosiella nattrassii)；
Ash arrhizus bacteria (Botrytis cinerea), the Powdery Mildew (Sohaerotheca humuli), anthrax bacteria of " grass is mould " (Colletotrichum acutatum), epidemic disease bacterium (Phytophthora cactorum)；
The neckrot germ (Botrytis allii) of " onion ", ash arrhizus bacteria (Botrytis cinerea), hickie leaf spoting bacteria (Botrytis squamosa), Pseudoperonospora cubensis (Peronospora destructor)；
The root nodule germ (Plasmodiophora brassicae) of " cabbage ", Bacteria erwinia (Erwinia Carotovora), Pseudoperonospora cubensis (Peronospora parasitica)；
Hyphal cluster germ (Sclerotinia sclerotiorum), the ash arrhizus bacteria (Botrytis cinerea) of " hyacinth bean "；
The Powdery Mildew (Podosphaera leucotricha) of " apple ", black star germ (Venturia inaequalis), Blossom rot bacterium (Monilinia mali), stain germ (Mycosphaerella pomi), rotten pathogenic bacteria (Valsa mali), Spot defoliation bacterium (Alternaria mali), brown spot pathogen (Gymnosporangium yamadae), Target spot pathogen (Botryosphaeria berengeriana), anthrax bacteria (Glomerella cingulata), brown patch germ (Diplocarpon mali), fly pinta bacterium (Zygophiala jamaicensis), sooty mould bacterium (Gloeodes Pomigena), leaf spot fungi；
Powdery Mildew (Phyllactinia kakicola), the anthrax bacteria (Gloeosporium kaki), angle spot of " persimmon " It falls leaves germ (Cercospora kaki)；
The brown rot germ (Monilinia fructicola) of " peach ", black star germ (Cladosporium carpophilum), Phomopsis germ (Phomopsis sp.)；
The brown rot germ (Monilinia fructicola) of " cherry "；
Ash arrhizus bacteria (Botrytis cinerea), the Powdery Mildew (Uncinula necator), late blight bacterium of " grape " (Glomerella cingulata), Colletotrichum acutatum), Pseudoperonospora cubensis (Plasmopara Viticola), hemorrhagic black smallpox germ (Elsinoe ampelina), brown patch germ (Pseudocercospora vitis), black rot Bacterium (Guignardia bidwellii)；
It is the black star germ (Venturia nashicola) of " pears ", brown spot pathogen (Gymnosporangium asiaticum), black Pinta bacterium (Alternaria kikuchiana), Target spot pathogen (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali)；
The zonate spot bacterium (Pestalotia theae) of " tea ", anthrax bacteria (Colletotrichum theae- Sinensis)；
The Streptomyces scabies (Elsinoe fawcetti) of " citrus ", mould germ (Penicillium italicum), green mould Bacterium (Penicillium digitatum), ash arrhizus bacteria (Botrytis cinerea), stain germ (Diaporthe Citri), ulcer bacteria (Xanthomonas campestris pv.Citri)；
The cereal Powdery Mildew (Erysiphe graminis) of " wheat ", cereal reaping hook germ (Fusarium Graminearum), mountain top reaping hook germ (F. culmorum), avenge mould leaf spoting bacteria (Microdochium nivale), wheat Stripe Rust (Puccinia striiformis), stem rust of wheat bacterium (P. graminis), wheat leaf rust germ (P.recondita), rotten reaping hook germ (Micronectriella nivale), wheat loose smut bacterium (Ustilago Tritici), black crooked germ (Tilletia caries), wheat Phyllostachys pubescens (Pseudocercosporella Herpotrichoides), wheat septoria bacterium (Mycosphaerella graminicola), many spores of clever withered shell bacterium (Stagonospora nodorum), wheat De Shi mould bacterium (Pyrenophora tritici-repentis), rhizoctonia solani (Gaeumanomyces graminis), Streptomyces scabies (Fusarium graminearum)；
The cereal Powdery Mildew (Erysiphe graminis) of " barley ", cereal reaping hook germ (Fusarium Graminearum), fusarium avenaceum (F. avenaceum), mountain top reaping hook germ (F. culmorum), avenge mould leaf spoting bacteria (Microdochium nivale), puccinia striiformis (Puccinia striiformis), stem rust of wheat bacterium (P. Graminis), barley leaf rust bacterium (P.hordei), naked smut bacterium (Ustilago nuda), barley leaf blotch bacterium (Rhynchosporium secalis), net blotch of barley bacterium (Pyrenophora teres), barley A. mali (Cochliobolus sativus), stripe disease of barley bacterium (Pyrenophora graminea), sheath blight fungus (Rhizoctonia solani);
The rice blast (Pyricularia oryzae) of " rice ", banded sclerotial blight (Rhizoctonia solani), bakanae disease (Gibberella fujikuroi), flax leaf spot (Cochliobolus niyabeanus), seedling blight bacterium (Pythium Graminicola), leaf spot bacteria (Xanthomonas oryzae), bacillary seedling blight bacterium (Burkholderia Plantarii), the withered bacillary germ (Burkholderia glumae) of brown streak bacterium (Acidovorax avenae), paddy, sheath Blight bacterium (Pyricularia oryzae), striped germ (Cochiobolus Miyabeanus)；
The sclerotinite (Sclerotinia sclerotiorum) of " rape straw ", sheath blight fungus (Rhizoctonia solani)；
The sclerotiniose (Sclerotinia sclerotiorum) of " tobacco ", powdery mildew (Erysiphe cichoracearum), Tobacco brown spot pathogen (Alternaria longipes), two spore powdery mildews (Erysiphe cichoracearum), tobacco anthrax Germ (Colletotrichum tabacum), Peronosporatabacina (Peronospora tabacina), the black chamber germ of tobacco (Phytophthora nicotianae)；
The gray mold (Botrytis cinerea) of " tulip "；
Snow big grain sclerotinia rot (Sclerotinia borealis), the samping off (Pythium of " Western Korea lawn grass " Aphanidermatum)；
The powdery mildew (Erysiphe graminis) of " orchard grass "；
The Cercospora kikuchii (Cercospora kikuchii) of " soybean ", soybean Elsinochrome (Elsinoe glycines), Seat shell soya bean varieties (Diaporthe phaseolorum var.sojae), Soybean Brown Spot line septoria musiva (Septoria Glycines), Germ To Soybean Frogeye Leaf Spot (Cercospora sojina), Phakopsora pachyrhizi (Phakopsora pachyrhizi), Pathogen of soybean root rot (Phytophthora sojae), sheath blight fungus (Rhizoctonia solani), stick spore leaf spot (Corynespora casiicola), sclerotinite (Sclerotinia sclerotiorum)；
The smut (Ustilago maydis) of " corn ", different cochliobolus (Cochliobolus heterostrophus), Jowar cercospora glue bacterium (Gloeocercospora sorghi), Puccinia polysora (Puccinia polysora), grey leaf spot disease of maize Bacterium (Cercospora zeae-maydis), sheath blight fungus (Rhizoctonia solani)；
The phytophthora infestans (Phytophthora infestans) of " potato ", potato phytophthora pink disease bacterium (Phytophthora erythroseptica), powdery scab of potato bacterium (Spongospora subterranean)；
Humulus Pseudoperonospora cubensis Pseudoperonospora bacterium (Pseudoperonospora humuli);
Alternaric bacteria (Alternaria japonica), the hickie germ (Cercosporella of brassicaceous vegetable Brassicae), plasmodiophora brassicae (Plasmodiophora brassicae), Peronospora parasitic bacterium (Peronospora parasitica)；
Onion mildew bacterium (Peronospora destructor);
Downy mildew of spinach bacterium (Peronospora spinaciae)；
Plurality of plant diseases: level ground grass Pythium aphanidermatu (Pythium aphanidermatum), Pythium debarianum, P.graminicola Subram (Pythium graminicola), abnormal female pythium spp (Pythium irregulare), Pythium ultimum bacterium (Pythium ultimum), the pathogen of Botrytis cinerea (Botrytis cinerea), sclerotinite (Sclerotinia sclerotiorum)。
Bactericidal composition of the invention is especially effective to the disease of following crop: brown spot, spot defoliation on apple, Ring spot, anthracnose, shot hole；Powdery mildew, the gray mold of more crops；Rice sheath blight disease, rice blast, false smut；Chinese cabbage blackspot Disease；Lawn giant spot disease；Wheat leaf rust；Wildfire, balck shank；Chinese yam root rot；Cucumber downy mildew.
Bactericidal composition of the invention is suitable for the prevention and treatment disease as caused by bacterium as follows: for example by pseudomonas (Pseudomonas), disease caused by xanthomonas (Xanthomonas), Erwinia (Erwinia) etc..
Example as the disease as caused by bacterium has:
Disease caused by pseudomonas (Pseudomonas), as the bacillary spot blight of cucumber, tobacco pyrotechnics are sick (by cloves Pseudomonad Pseudomonas syringae pv. Iachrymans) cause)；The bacterial wilt of tomato is (by Solanaceae Lei Er Salmonella (Ralstonia solanacearum) causes)；Rice plants bacillary cereal blight (grain rot) (by Glume blight bacterium (Burkholderia glumae) causes)；
The disease as caused by xanthomonas (Xanthomonas), if the black rot of wild cabbage is (by xanthomonas campestris (Xanthomonas campestris) causes)；The Bacterial Leaf Blight of rice plants is (by bacterial blight of rice Flavobacterium (Xanthomonas oryzae) causes) and citrus fruit trees canker (cankar) (by citrus ulcer bacterium (Xanthomonas citri) causes)；The disease as caused by Erwinia (Erwinia), such as the bacterial soft rot of wild cabbage (being caused by carrot soft rot Erwinia (Erwinia carotovora)) etc..
The crop of bactericidal composition processing of the invention is such as but not limited to cereal, bean, fruit tree and water Fruit, greengrocery, root vegetables, insutrial crop or crop for industrial processes, forage grass class, lawn class or turf, fragrance Crop, flowers etc..
Bactericidal composition of the invention can also be used to prevent and treat fruit vegetables storing period disease.Such as the fruit as caused by following pathogen It is real to rot:
Aspergillus bacterium, such as aspergillus flavus；
Botrytis (Botrytis) strain, such as Botrytis cinerea (Botrytis cinerea);
Penicillium (Penicillium) strain, for example, penicillium expansum (Penicillium expansum) and P.purpurogenum;
Sclerotinia (Sclerotinia) strain, such as sclerotinite (Sclerotinia sclerotiorum);
Verticillium (Verticilium) strain, such as black and white wheel branch spore (Verticilium alboatrum).
Bactericidal composition of the invention be also particularly well suited for prevention and treatment fruit vegetables storing period disease blossom-end rot, green mould, penicilliosis, Anthracnose.
Fungicidai mixtures of the invention are further adapted for prevention and treatment kind biography and soil-borne disease.Such as kind of the biography as caused by following pathogen The rotten and wilting disease and seedling disease passed with soil:
Alternaria belongs to kind, such as rape life rod method (Alternaria brassicicola);
Aphanomyces (Aphanomyce) category kind, such as Kidney bean silk capsule are mould (Aphanomyces euteiches);
Ascochyta (Ascochyta) belongs to kind, such as Ascochyta lentis;
Aspergillus, such as aspergillus flavus;
Cladosporium belongs to kind, such as draft branch spore (Cladosporium herbarum);
Cochliobolus belongs to kind, such as standing grain cochliobolus;
Colletotrichum belongs to kind, such as hair core anthrax-bacilus (Colletotrichum coccodes);
Fusarium belongs to kind, such as yellow fusarium;
Gibberella category kind, such as beautiful another name for Sichuan Province multitude are red mould;
Shell ball spore category (Macrophomina) belongs to kind, such as Kidney bean shell ball spore (Macrophominaphaseolina);
Small setting-out shell category strain, such as the rotten small setting-out shell of snow;
Penicillium belongs to kind, such as penicillium expansum;
Phaeosphaeria strain, such as Phaeosphaeria nodorum;
Phoma (Phoma) belongs to kind, such as black embryo Phoma sp (Phoma lingam);
Phomopsis (Phomopsis), such as soybean Phomopsis (Phomopsis sojae);
Phytophthora belongs to kind, such as Phytophthora cactorum (Phytophthora cactorum);
Pyrenophora belongs to kind, such as wheat nuclear cavity bacteria (Pyrenophora graminea);
Pyricularia Sacc. (Pyricularia) belongs to kind, such as Magnaporthe grisea (Pyricularia oryzae);
Pythium kind, such as Pythium ultimum;
Rhizoctonia belongs to kind, such as Rhizoctonia solani Kuhn;
Rhizopus (Rhizopus) belongs to kind, such as Rhizopus oryzae (Rhizopus oryzae);
Bulbil category (Sclerotium) belongs to kind, such as Sclerotium rolfsii (Sclerotium rolfsii);
Core coral Pseudomonas (Typhula) belongs to kind, such as meat spore core coral bacterium (Typhula incarnata);
Verticillium (Verticillium) belongs to kind, such as verticillium dahliae (Verticillium dahliae).
Bactericidal composition of the invention can spread by seed treatment, cauline leaf, soil application or used for ponds etc. Prevention and treatment for the various diseases generated when cultivation includes the agriculture garden crop of flowers, lawn, herbage.
The present invention provide it is a kind of prevent and treat plant pathogen method, by the bactericidal composition act on pathogenic bacteria and/ Or in its environment or plant, plant parts, plant propagation material, soil, region, material or space.
The present invention also provides a kind of control plant, plant part, plant propagation material and the subsequent plant organs that grow The method of plant pathogenic fungi, including by bactericidal composition of the invention with agronomy is effective and the amount of application of basic plant-less toxicity It applied, be impregnated with seed treatment, foliage applying, stem, instiled, is poured, sprayed, is sprayed, dusting, the methods of distribution or smoke application It is growing or is needed in the soil or cultivation medium wherein grown to plant, plant part, plant propagation material or plant.
Bactericidal composition of the invention can be used for the plant device protecting plant part, plant propagation material and then growing Official.
Bactericidal composition of the invention can handle all plants and plant part." plant " refers to all plants and plant Population, such as ideal and undesirable wild plant, cultivated plant and plant variety are (regardless of whether by plant variety or plant Cultivate the protection of human rights benefit).Cultivated plant and plant variety can be the plant obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique methods, such as use dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Part and organ above and below the ground, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.It crop and nourishes and generates and case of propagation material, such as transplants, bulb, rhizome, running roots Plant part is also belonged to seed.
Term " plant propagation material " is interpreted as referring to all plant parts for having fertility, such as seed, can use In breeding the latter and vegetable matter such as cuttage item or stem tuber (such as potato).Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.To The germination plant and effective plant inhibited after germinateing in soil or after emergence.Young plant can be before transplantation by being impregnated into Row whole or Local treatment are protected.
Currently preferred propagation material is seed.The method of seed treatment, such as can there are, diluent liquid or solid Seed is directly immersed in liquid condition solution the method for making medicament be impregnated with seed without dilution, by solid by the medicament of shape Medicament or liquid preparation are mixed with seed, and being coated processing makes the method for the surface of the seed attachment medicament, in plantation The methods of sprinkling near seed simultaneously.
Plant part and the plant organ then grown are any of the plant generated by plant propagation material such as seed Part.Plant part, plant organ and plant also can benefit from by the way that bactericidal composition is applied to plant propagation material institute The pathogenic damage of acquisition is protected.The plant organ grown behind certain plants part and certain places can also regard plant propagation as Material, its own can apply (or processing) with bactericidal composition；To by processed plant part and processed plant Plant, other plant parts and the other plant organs that organ generates also can benefit from by applying bactericidal composition With.
Seed and seedling are subject to the harm of a variety of pathogens and infect, and are the links of most fragile in process of crop growth； Are there is sick seedling, seedling death phenomenon by pathogen infection in seed, seedling, can be reduced effective strain number of unit area crop, be caused crop It drops in production over a large area and quality decline.
Bactericidal composition of the invention be suitable for protect plant propagation material such as seed, fruit, stem tuber or kernel or Person's plant cutting is not infected by phytopathogen.Can the propagation material, example be handled with the bactericidal composition before administration As dressed seed prior to seeding.Can also by liquid composition impregnate kernel or with solid composite be coated with kernel, by activity at It gives and is added in kernel (coating).It, can also should when propagation material is applied to that for example seed is broadcast in ditch in seeding process Composition is applied to application site.These plant propagation materials handled to the processing method of plant propagation material and so are all Further subject matter of the present invention.
It, can also be by being impregnated in succession with the liquid preparation of each active constituent in order to handle plant propagation material especially seed Stem tuber or grain, or by being coated with the wet or dry preparation combined.
Seed treatment application of the invention by prior to seeding and/or after vernalization by seed is sprayed or and dusting into Row.
Bactericidal composition of the invention is applied to seed with itself or suitable dosage form.It is preferred that seed is in stable state Under it is processed so that processing do not cause any damage.It usually can be any between harvesting and sowing to the processing of seed Time carries out.
It is preferred that being handled before seed sowing, so as to be pre-processed with bactericidal composition to the seed broadcasted sowing. Specifically, preferably by seed pelleting or by seed-pelleting in bactericidal composition processing of the invention.Processing as a result, Active constituent in the bactericidal composition is attached on seed, so as to the prevention and control for disease.
By using seed treatment compositions processing seed to protect seed and rudiment plant to invade from plant-pathogenic bacterium Dye is known for a long time and is to continuously improve.However, processing seed also bring it is a series of be not can find with Satisfactory manner solves the problems, such as.Therefore, it is necessary to develop the method for protection seed and rudiment plant, it can exempt and plant Additionally apply crop protection products again afterwards or after plant germination.In addition, it is also necessary to optimize the amount of reactive compound used, so as to Best protection is provided to seed and rudiment plant, to prevent the invasion of plant pathogen, and reactive compound used will not damage Evil plant itself.
But, it is undesirable to effect be the germination rate that processed seed is reduced using seed treatment compositions.Generally Ground can be reduced with the germination rate of the processed seed of seed treatment compositions with the time after application seed treatment, in It is to limit the shelf life of processed seed.Current way is the selection of time of seed treatment in such as field seed Processing will sow preceding processing, to avoid the serious damage to crop.On the other hand, by seed be exposed to it is for example cold, It may aggravate the toxicity damage for the plant that agricultural chemicals generate seed under the environmental pressures such as dry.
It has to be noticed that being applied to the amount of the bactericidal composition of the present invention of seed and/or other usually during seed treatment The amount of additive is selected as the germination that will not influence seed or will not damage plant.
Bactericidal composition of the invention is for processing plant propagation material, especially rice, rape, cotton, wheat, big Wheat, soybean, corn, peanut seed be particularly advantageous.
The present invention also provides a kind of methods for protecting seed comprising after making seed prior to seeding and/or vernalization and originally The bactericidal composition of invention contacts.
Therefore, more particularly, the present invention a kind of using bactericidal composition processing seed of the invention to protect seed and The method that germinating plants are invaded and harassed from plant pathogen.
Moreover, it relates to which bactericidal composition of the invention is for handling seed to protect seed and germinating plants to exempt from The purposes infected by phytopathogenic fungi.
Moreover, it relates to be protected from the kind of plant pathogen invasion through bactericidal composition processing of the invention Son.
Bactericidal composition of the invention can also be used to prevent or control a variety of pathogenic or saprophytic in soil or cultivation medium Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), Botrytis cinerea (Botrytis cinerea), Cercospora (Cercospora spp.), ergot (Claviceps purpurea), standing grain cochliobolus (Cochliobolus sativus), Colletotrichum (colletotrichum Spp.), Epicoccum (Epicoccum spp.), F.graminearum schw (Fusarium graminearum), rice dislike seedling chain spore (Fusarium moniliforme), fusarium oxysporum (Fusarium oxysporum, fusarium moniliforme (Fusarium Proliferatum), fusariun solani (Fusarium solani) is tieed up glue chain spore (Fusarium subglitinans), and length is compacted Spore category (Helminthosporium spp) avenges rotten microtorr bacterium (Microdochium nivale), Penicillium (Pencillium Spp), Phoma (Phoma spp.), wheat nuclear cavity bacteria (Pyrenophora graminea), rice blast Pyricularia Sacc. (Pyricularia oryzae), Rhizoctonia solani Kuhn (Rhizoctonia solani), Rhizoctonia cerealis (Rhizoctonia Cerealis), Sclerotinia (Sclerotinia spp.), Septoria (Septoria spp.), silk axis smut (Sphacelotheca reilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula Incarnate), hidden smut (Urocystis occulta), Ustilago (Ustilago spp.) or Verticillium (Verticillium spp.)。
Soil germ has alternaric bacteria, rhizoctonia solani, sickle-like bacteria, phytophthora, bacterium of dampinging off, root rot, pythium spp, grey mold Bacterium, soft-rot fungi etc..Under normal circumstances, soil germ can generate a large amount of thallus, if condition it is advantageous to pathogen growth development and Host be again it is susceptible, germ mass propagation and can infect host, in the presence of susceptible host, these germs Into the lasting pathogenic phase, with the continuous cropping of crop, mass propagation is spread, but later nutrient be depleted or edaphic condition such as When temperature, humidity etc. are unfavorable to germ, germ can enter dormant period again.In the absence of susceptible host, soil-borne disease bacterium is in soil It can also be survived in earth, in addition to soil germ has extensive host range, moreover it is possible to be fallen in the root surface of non-host or residual branch It survives on leaf, is undivided with it with saprophytic competitiveness.But different germs be it is discrepant, as sickle-like bacteria, rod method Bacterium can almost be survived in the soil with indefinite duration.
Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, such as: soil, water etc., Specific raw material can be used for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Sawdust, bark etc..
The method that medicament is applied into soil, including " bactericidal composition " is sprayed on soil method, by " Bactericidal composite The method and " bactericidal composition " is irrigated into the method into soil that object " is mixed with soil.
Therefore, the present invention also provides the bactericidal compositions to be used to handle the soil of plant growth to protect the plants from The purposes of pathogenicity harmful fungoid or bacterial invasion in soil.
A method of prevention and treatment plant pathogen, this method include by bactericidal composition of the invention with agronomy effectively and base The amount of application of this plant-less toxicity applied with seed treatment, foliage applying, stem, is impregnated with, instiled, is poured, sprayed, is sprayed, dusting, The methods of distribution or smoke are applied to plant, plant propagation material and the plant organ then grown, cultivation medium, material or sky Between in.
Therefore, the present invention provides a kind of method for preventing and treating plant pathogen, and this method includes by sterilization of the present invention Composition is applied to plant leaf surface.
The present invention provides a kind of method for preventing and treating plant pathogen, and this method includes by bactericidal composition of the present invention The plant organ for being applied to plant propagation material and then growing.
The present invention provides a kind of method for preventing and treating plant pathogen, and this method includes by bactericidal composition of the present invention It is applied to cultivation medium.
The present invention provides a kind of method for preventing and treating plant pathogen, and this method is included in plant and before disease infestation or is invaded After dye by the bactericidal composition act on plant, plant propagation material and then grow plant organ, cultivation medium, In material or space.
A method of prevention and treatment plant pathogen, this method include biphenyl pyrazine bacterium amine and copper calcium sulfate are administered simultaneously or Sequential application.
Another object of the present invention provides a kind of control plant, plant part, plant propagation material and then grows Plant organ, plant culture medium plant pathogenic fungi method, this method include by bactericidal composition of the invention with Agronomy is effective and the amount of application of basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instiled, be poured, sprayed Penetrate, be sprayed, dusting, the methods of distribution or smoke be administered to plant, plant part, plant propagation material or plant be growing or It needs in the cultivation medium wherein grown, preferably soil.
Bactericidal composition of the invention can be applied by different processing methods, these methods for example:
Liquid comprising the bactericidal composition is sprayed onto the aerial part of the plant；
Dusting mixes particle or powder in the soil, sprays in the surrounding plants, and the tree injection or smearing the case where Under；
Cladding or film coated are carried out to the seed of plant.
When for postharvest fruit and vegetable anti-corrosive fresh-keeping, 200-2000 times of liquid is usually diluted with water, is leached after soaking fruit.
The present invention provides a kind of method for preventing and treating plant pathogen, can be treatment, prevention or method of eradication.
The invasion of bactericidal composition of the invention for disease described in protection plant resistant in certain time after treatment. The time of protective effect obtained usually is from plant and has handled continuity 1 to 10 day, preferably 1 to 7 day by composition.Or For up to 200 days after handling seed.
Bactericidal composition of the invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid The dosage forms such as agent, granule, seed coat agent use, amount of application, according to the mixing ratio, meteorological condition, medicament of effective component Form, Dressing date, method of administration, application place, prevent and kill off object noxious livings, objective crop etc. it is different and variant.
A method of prevention and treatment plant pathogen, biphenyl pyrazine bacterium amine and copper calcium sulfate are administered simultaneously or applied respectively, Or sequential application.
Processing method of the invention can also be used in handle propagation material such as stem tuber or rhizome, and can be used for handling seed, Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for processing root.The present invention Processing method can also be used for the aerial part of processing plant such as dry, stem or stalk, leaf, flower and fruit in relation to plant.
Generally for leaf portion processing: 0.1-10000g/ha, preferably 10-1000 g/ha, more preferable 50-500 g/ha；It is right For dipping or application of instiling, the dosage can also even be reduced, especially when application inert base such as asbestos or pearl When rock；
For seed treatment: 2-5000g/100kg seed, preferably 3-1000g/100kg seed；
For soil or processing used for ponds: 0.1-10000g/ha, preferably 1-1000g/ha；
It is fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, is leached after soaking fruit.
Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to practical feelings when reality is applied Condition and needs adjust rate of application especially in accordance with the seriousness of the property and germ of plant to be processed or crop.
Bactericidal composition of the invention can also be used to that Stored Product be protected to infect from germ.The suitable example of the product Including stem, leaf, seed, fruit or cereal, can be protected under fresh harvesting state or when processing, as predrying, humidification, It crushes, grind, squeeze out or toasts.
Bactericidal composition of the invention shows good Fungicidally active, and the Fungicidally active of single active constituent compared with Weak, the activity of bactericidal composition of the invention is more than the active simple adduction of each single compound
Biphenyl pyrazine bacterium amine of the invention combines/combined administration with copper calcium sulfate.Including separating, sequentially or simultaneously applying biphenyl pyrrole Piperazine bacterium amine and copper calcium sulfate.Preferably, the biphenyl pyrazine bacterium amine and copper calcium sulfate group are combined into comprising biphenyl pyrazine bacterium amine and sulphur The form of the composition of sour copper calcium.
Bactericidal composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition Ingredient can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with Based on dosage form provided by the invention.
As a further improvement of the present invention, bactericidal composition of the invention can be configured to any agent agriculturally allowed Type.
As a further improvement of the present invention, the dosage form of bactericidal composition of the invention be aerosol, it is capsule suspension, cold It is atomized concentrating agents, heat atomization concentrating agents, CG/Encapsulated granule, granula subtilis, instant solution, sprayable pulvis, emulsifiable concentrating agents, water Oil-in emulsion, water-in-oil emulsion, bulky grain agent, fine granule, the dispersible powder of oil, the miscible flowable concentrating agents of oil, Oily miscible liquids, foaming agent, paste, suspension concentrating agents, solvable concentrating agents, suspending agent, seed coat agent, wettable powder, moisture Shot agent, soluble powder, microcapsule suspending agent, coated granule, squeeze out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, The cold atomization preparation of ultra low volume liquids, suspoemulsion, ultra-low volume, super-low capacity calorimetric atomization preparation, double pack (twin pack), Seed treatment dry powder doses, seed treatment emulsion, seed treatment suspending agent, seed treatment liquor, seed treatment dispersible pulvis, kind Subprocessing microcapsule suspending agent, suspoemulsion, milk particle agent, ultra-low volume suspending agent, ultra low volume liquids, can divide seed treatment gel Dissipate property concentrating agents.
It is living comprising biphenyl pyrazine bacterium amine and copper calcium sulfate, filler and/or surface in bactericidal composition of the present invention Property agent.
In the bactericidal composition, the biphenyl pyrazine bacterium amine and copper calcium sulfate quality sum account for the bactericidal composition The 5%-90% of quality, more preferable 10%-80%, more preferable 20%-75%, more preferable 20%-70%.
According to the present invention, term " filler ", which refers to, can be combined or be combined with reactive compound to make it easier for being administered to The natural or synthetic organic or inorganic compound of object (such as plant, crop or careless class).Therefore, the filler is preferably It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.
The nonactive medium that can be used in the present invention can be used as solid matchmaker either solid is also possible to liquid What dielectric material used has for example: phyteral powdery type (such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut Shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem particle, the residue etc. after extracting plant essence), paper It opens, sawdust, crushes synthesized polymer body, the clay class (such as kaolin, bentonite, acid china clay etc.), talcum powder of synthetic resin etc. Class.Silica (such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates), active carbon, natural mineral matter class (float stone, Lv Po Thread stone and zeolite etc.), fire diatomite, sand, (such as polyethylene, polypropylene, the polyvinylidene chloride etc.), chlorination such as plastics medium Chemical fertilizer, the soil of the inorganic mineral powder of potassium, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, ammonium chloride etc. Fertilizer, these substances can be used alone or two or more is mixed.
It can be used as can selecting in llowing group of materials for liquid media materials'use, such as water, alcohol type (such as first Alcohol, ethyl alcohol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng), ethers (such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.), aliphatic hydrocarbon class (such as kerosene, Mineral oil etc.), aromatic hydrocarbons class (such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro rouge Fat hydrocarbon, chlorobenzene, etc.), halogenated hydrocarbon class, amides, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal oil etc..
To make the emulsification of effective component compound, dispersion and/or wetting, surfactant can be used for example can be with Enumerate fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the aliphatic ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as object, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine combination.
To make effective component compound dispersion stability, attachment and/or combination, such as xanthan gum, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid (such as cephalin and lecithin) with And the adjuvants such as synthetic phospholipid, bentonite, sodium lignin sulfonate.
The wherein optional spent glycol of antifreezing agent, propylene glycol, glycerine, sorbierite.Deflocculant as suspension product The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.
Organic silicon defoamer can be used in defoaming agent.
The colorant that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff: alizarin dyes, azo dyes and metallized phthalocyanine dye；And microelement, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.
Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.
The reactive compound can be mixed preparation with conventional additives by known way by the preparation of the invention. The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersing agent, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, defoaming agent, preservative, increasing Thick dose, water and other processing aids.
Bactericidal composition of the invention not only includes can be by suitable equipment as spraying or dusting equipment is suitable for immediately Object to be processed, but also including needing the concentration commercial composition being diluted before being applied to object.
The bacterium amine of pyrazine containing biphenyl of the invention and copper calcium sulfate can also be applied with other active ingredient combinations, such as Expand activity profile or prevents from forming resistance.Other active constituents such as fungicide, bactericide, attractant, insecticidal Agent, acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..
Reactive compound biphenyl pyrazine bacterium amine and copper calcium sulfate can be administered simultaneously, or apply respectively or sequential application, separate Sequence when application is on the result of prevention and treatment usually without influence.
Composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition at Dividing can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.
For bactericidal composition of the invention in the case where reducing reactive compound application total amount, having to harmful fungoid improves activity (synergy).And bactericidal composition of the invention shows that the bacterium of tolerance also has excellent kill to existing fungicide Bacterium effect.
A kind of bactericidal composition of the present invention, the composition are answered by the way that biphenyl pyrazine bacterium amine and copper calcium sulfate are carried out binary Match, so that obtained composition has gain effect in control efficiency, and expanded fungicidal spectrum, plays what a medicine was mostly used Effect, effectively slows down or germ is avoided to develop drug resistance.The bactericidal activity of bactericidal composition of the invention is than each active ingredient The active adduction of object is considerably higher, and there are unpredictable, necessary being synergistic effects, rather than just active increasing It mends.
When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright It is aobvious.But the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.
Specific embodiment
First passing through specific example of formulations below, the invention will be further elaborated.
Example of formulations
1: 71% biphenyl pyrazine bacterium amine copper calcium sulfate wettable powder of embodiment
Biphenyl pyrazine bacterium amine 1%, copper calcium sulfate 70%, lignosulfonates 5%, spaonin powder 4%, attapulgite add to 100%, will The mixing of aforementioned formula material, uniform stirring, remixes uniformly after airslide disintegrating mill, obtains 71% biphenyl pyrazine in a stirring kettle Bacterium amine copper calcium sulfate wettable powder.
Embodiment 2:50.5% biphenyl pyrazine bacterium amine copper calcium sulfate wettable powder:
Biphenyl pyrazine bacterium amine 0.5%, copper calcium sulfate 50%, naphthalene sulfonic acid-formaldehyde condensation product 8%, lauryl sodium sulfate 5%, kaolin 100% is added to, aforementioned formula material is mixed, in a stirring kettle uniform stirring, is being uniformly mixed, is being made after airslide disintegrating mill 50.5% biphenyl pyrazine bacterium amine copper calcium sulfate wettable powder.
Embodiment 3:44% biphenyl pyrazine bacterium amine copper calcium sulfate wettable powder
Biphenyl pyrazine bacterium amine 4%, copper calcium sulfate 40%, MODIFIED LIGNOSULPHONATE 5%, lauryl sodium sulfate 5%, diatomite adds To 100%, aforementioned formula material is mixed, in a stirring kettle uniform stirring, be uniformly mixed after airslide disintegrating mill, be made 44% Biphenyl pyrazine bacterium amine copper calcium sulfate wettable powder.
Embodiment 4:51% biphenyl pyrazine bacterium amine copper calcium sulfate water dispersible granules
Biphenyl pyrazine bacterium amine 1%, copper calcium sulfate 50%, alkyl benzene sulfonate calcium salt 10%, lauryl sodium sulfate 5%, ammonium sulfate 2%, Bentonite adds to 100%, and aforementioned formula material is uniformly mixed, and with micro jet, is mediated, then addition fluidized bed is made 51% biphenyl pyrazine bacterium amine copper calcium sulfate moisture is mixed to prepare through sampling analysis after being granulated in grain drying machine, dry, sieve Shot agent.
Embodiment 5:61% biphenyl pyrazine bacterium amine copper calcium sulfate water dispersible granules
Biphenyl pyrazine bacterium amine 1%, copper calcium sulfate 60%, aliphatic acid polyethenoxy ether 5%, lauryl sodium sulfate 4%, kaolin adds To 100%, aforementioned formula material is uniformly mixed, with micro jet, is mediated, fluidized-bed granulation dryer is then added In be granulated, dry, sieve after through sampling analysis be mixed to prepare 61% biphenyl pyrazine bacterium amine copper calcium sulfate water dispersible granules.
Embodiment 6:49.7% biphenyl pyrazine bacterium amine copper calcium sulfate water dispersible granules
Biphenyl pyrazine bacterium amine 0.7%, copper calcium sulfate 49%, octylphenol polyethylene oxyethylene group ether sulfate 5%, neopelex 5%, sodium carbonate 2%, white carbon black adds to 100%, and aforementioned formula material is uniformly mixed, and with micro jet, is mediated, then It is added in fluidized-bed granulation dryer after being granulated, drying, sieve and obtains 49.7% biphenyl pyrazine bacterium amine through sampling analysis Copper calcium sulfate water dispersible granules.
Embodiment 7:60.5% biphenyl pyrazine bacterium amine copper calcium sulfate suspending agent
Biphenyl pyrazine bacterium amine 0.5%, copper calcium sulfate 60%, polycarboxylate 6%, silicone oil 0.3%, xanthan gum 0.9%, diethylene glycol (DEG) 2.5%, Deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to crossing effective component, by high speed shear It is uniformly mixed, effective component is added, ball milling 2 ~ 3 hours, make partial size all at 5 μm hereinafter, obtaining 60.5% in the ball mill Biphenyl pyrazine bacterium amine copper calcium sulfate suspending agent.
Embodiment 8:70.5% biphenyl pyrazine bacterium amine copper calcium sulfate suspending agent
Biphenyl pyrazine bacterium amine 0.5%, copper calcium sulfate 70%, naphthalene sulfonic acid-formaldehyde condensation product 6%, hydroxyethyl cellulose 0.7%, silicone Compound 0.1%, polyethylene glycol 2%, deionized water adds to 100%, by its after thickener and antifreezing agent mixing in addition to effective component Remaining component is uniformly mixed by high speed shear, effective component is added, ball milling 2 ~ 3 hours, make partial size all 5 in the ball mill μm hereinafter, obtaining 70.5% biphenyl pyrazine bacterium amine copper calcium sulfate suspending agent.
Embodiment 9:40.2% biphenyl pyrazine bacterium amine copper calcium sulfate suspending agent
Biphenyl pyrazine bacterium amine 0.2%, copper calcium sulfate 40%, lignosulfonates 8%, aluminum magnesium silicate 1%, silicone 0.2%, glycerine 2.8%, deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to effective component, by cutting at a high speed Cut it is uniformly mixed, be added effective component, ball milling 2 ~ 3 hours, make partial size all at 5 μm hereinafter, obtaining in the ball mill 40.2% biphenyl pyrazine bacterium amine copper calcium sulfate suspending agent.
Embodiment 10:78% biphenyl pyrazine bacterium amine copper calcium sulfate suspoemulsion
Biphenyl pyrazine bacterium amine 1%, copper calcium sulfate 77%, N-Methyl pyrrolidone 10%, alkyl benzene sulfonate calcium salt 5%, methylcellulose 1%, propylene glycol 3%, Tween 80 5%, water adds to 100%；Finely ground sulfuric acid is added in the continuous phase of the bacterium amine of pyrazine containing biphenyl The suspended phase of copper calcium is mixed to prepare 78% biphenyl pyrazine bacterium amine copper calcium sulfate suspoemulsion.
Embodiment 11:61% biphenyl pyrazine bacterium amine copper calcium sulfate water dispersible granules
Biphenyl pyrazine bacterium amine 1%, copper calcium sulfate 60%, octylphenol polyethylene oxyethylene group ether sulfate 5%, neopelex 3%, sodium carbonate 1%, white carbon black adds to 100%, and aforementioned formula material is uniformly mixed, and with micro jet, is mediated, then It is added in fluidized-bed granulation dryer after being granulated, drying, sieve and obtains 61% biphenyl pyrazine bacterium amine through sampling analysis Copper calcium sulfate water dispersible granules.
12 55% biphenyl pyrazine bacterium amine copper calcium sulfate seed coat agent of embodiment
Biphenyl pyrazine bacterium amine 5%, copper calcium sulfate 50%, fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%, modification are wooden Plain sulfoacid calcium 3%, xanthan gum 1%, bentonite 1%.Glycerine 5%, PVP-K30 1%, complements to 100%, by above-mentioned each component press than Example is uniformly mixed, and is sanded, and 55% biphenyl pyrazine bacterium amine copper calcium sulfate seed coat agent is prepared into.
13 20.5% biphenyl pyrazine bacterium amine copper calcium sulfate coated granule of embodiment
Biphenyl pyrazine bacterium amine 0.5%, copper calcium sulfate 20%, polyethylene glycol 3%, the silicic acid 1% of high degree of dispersion, calcium carbonate complements to 100%, in a mixer, by levigate active component even spread to the carrier soaked by polyethylene glycol, it can obtain by this method Obtain the dust-free coated granules agent of 20.5% biphenyl pyrazine bacterium amine copper calcium sulfate.
Biphenyl pyrazine bacterium amine 2% and copper calcium sulfate 98% are uniformly mixed by embodiment 14.
Above embodiments proportion is weight per distribution ratio.
Biological test example:
Pesticide is made in the combination of active principles of different pesticides, is current exploitation and development novel pesticide and prevention and treatment agriculturally resistance A kind of effective and quick way of germ.After the pestsides synthesis of different cultivars, three kinds of type of action are typically exhibited: being added and make With, synergistic effect and antagonism.But it is specially which kind of effect, it is unpredictable, only just it can know that by many experiments.Compounding Synergy is formulated well, due to significantly improving practical control efficiency, the usage amount of pesticide is reduced, to greatly delay The generation of resistance.
One, virulence is tested:
The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture are calculated according to Sun Yunpei's Method, when CTC≤80, are then combined Object shows antagonism, and when 80<CTC<120, then composition shows summation action, and when CTC>=120, then composition shows Synergistic effect out.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
B in the percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture Percentage composition
Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100
Test 1: the toxicity test of powdery mildew of cucumber
It is spraying under 50PSI pressure with potter spray tower selected from the consistent cucumber seedling of growing way, every basin about 5mL, each medicine 12 concentration gradients are arranged in agent.Bacterium is connect after chemicals treatment for 24 hours, the powdery mildew of cucumber leaf for picking up from field is uniform above cucumber seedling Shake off conidium to be inoculated with, then cucumber seedling is put into greenhouse and is cultivated.It is classified after 7d according to the morbidity of powdery mildew of cucumber Standard complete stool investigates disease index, and calculates control efficiency, concentration EC50 in inhibiting then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).
Table 1
As known from Table 1, the weight proportion of prevention powdery mildew of cucumber, biphenyl pyrazine bacterium amine and copper calcium sulfate is the model of 1:10-1:400 When enclosing inner, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 2: the toxicity test of graw mold of tomato
Selected from the consistent tomato seedling of growing way, each processing selects 3 basins for trying leaf seedling, with potter spray tower under 50PSI pressure Spraying, 12 concentration gradients are arranged in every basin about 5mL, each medicament.Bacterium is connect after chemicals treatment for 24 hours, the tomato in field will be picked up from Gray mold leaf is uniformly shaken off conidium above tomato seedling and is inoculated with, and then tomato seedling is put into greenhouse and is cultivated.After 7d Disease index is investigated according to the morbidity grade scale complete stool of graw mold of tomato, and calculates control efficiency, then uses least square method Concentration EC50 in inhibiting is calculated, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 2: virulence test result of the present invention on prevention and treatment graw mold of tomato
As known from Table 2, the weight proportion of prevention graw mold of tomato, biphenyl pyrazine bacterium amine and copper calcium sulfate is the model of 1:10-1:400 When enclosing inner, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 3: the toxicity test of marssonina coronaria
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days marssonina coronarias will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 3: virulence test result of the present invention to marssonina coronaria
As known from Table 3, the weight proportion of prevention and treatment marssonina coronaria, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400's When in range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 4: the toxicity test of Alternaria mali
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days Alternaria malis will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 4: virulence test result of the present invention to Alternaria mali
As known from Table 4, the weight proportion of prevention and treatment Alternaria mali, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1: When in 400 range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 5: the toxicity test of barley powdery mildew bacteria
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days barley powdery mildew bacterias will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 5: virulence test result of the present invention to barley powdery mildew bacteria
As known from Table 5, the weight proportion of prevention and treatment barley powdery mildew bacteria, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400's When in range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 6: the toxicity test of wheat leaf rust germ
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days wheat leaf rust germs will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, use transfer needle Fungus block is moved to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture in 25 DEG C of incubators, every processing repeats 4 It is secondary.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony Two diameters are surveyed in right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 6: virulence test result of the present invention to wheat leaf rust germ
As known from Table 6, the weight proportion of prevention and treatment wheat leaf rust germ, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400 Range in when, co-toxicity coefficient is all larger than 120, both illustrates to show as synergistic effect within this range.
Test 7: the toxicity test of apple tree Streptomyces scabies
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days apple tree Streptomyces scabies will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, use transfer needle Fungus block is moved to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture in 25 DEG C of incubators, every processing repeats 4 It is secondary.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony Two diameters are surveyed in right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 7: virulence test result of the present invention to apple tree Streptomyces scabies
As known from Table 7, the weight proportion of prevention and treatment apple tree Streptomyces scabies, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400 Range in when, co-toxicity coefficient is all larger than 120, both illustrates to show as synergistic effect within this range.
Test 8: the toxicity test of Rhizoctonia solani Kuhn
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days Rhizoctonia solani Kuhns will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 8: virulence test result of the present invention to Rhizoctonia solani Kuhn
As known from Table 8, the weight proportion of prevention and treatment Rhizoctonia solani Kuhn, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400's When in range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 9: the toxicity test of tobacco smoke pollution
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days tobacco smoke pollutions will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 9: virulence test result of the present invention to tobacco smoke pollution
As known from Table 9, the weight proportion of prevention and treatment tobacco smoke pollution, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400's When in range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.
Test 10: the toxicity test of Chinese yam pine root fungus
Using inhibition mycelial growth rate method:
Biphenyl pyrazine bacterium amine and copper calcium sulfate are used into acetone solution respectively, then dilute preparation with 0.1% Tween-80 aqueous solution At the medical fluid of series of concentrations, 6mL is drawn respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of Ma Ling is added Potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, the toxic of 4 respective concentrations is made Culture medium；The biphenyl pyrazine bacterium amine of different ratio and copper calcium sulfate series of concentrations compound solution is made with same method toxic Culture medium.2 days Chinese yam pine root fungus will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:
Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.
Table 10: virulence test result of the present invention to Chinese yam pine root fungus
As known from Table 10, the weight proportion of prevention and treatment Chinese yam pine root fungus, biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400 Range in when, co-toxicity coefficient is all larger than 120, both illustrates to show as synergistic effect within this range.

Claims (10)

1. a kind of bactericidal composition, it is characterised in that: the bactericidal composition includes active constituent biphenyl pyrazine bacterium amine and sulfuric acid The weight proportion of copper calcium, the biphenyl pyrazine bacterium amine and copper calcium sulfate is 1:10-1:400, preferably 1:20-1:350, preferably 1: 30-1:300, more preferable 1:30-1:250；More preferably 1:30-1:200, more preferably 1:40-1:200, more preferably 1:50- 1:200, further preferably 1:50-1:180, further preferably 1:60-1:160, further preferably 1:70-1:150, into One step is preferably 1:80-1:150.
2. bactericidal composition according to claim 1, it is characterised in that: the biphenyl pyrazine bacterium amine and copper calcium sulfate quality The sum of account for the 5%-90%, more preferable 10%-80%, more preferable 20%-75%, more preferable 20%-70% of the Bactericidal composite amount of substance.
3. bactericidal composition according to claim 1, it is characterised in that: the dosage form of the bactericidal composition is aqueous suspension Agent, seed treatment suspending agent, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspending agent, granule, aqueous emulsion, effervesce Piece, thermal fog, dry suspending agent, oil-suspending agent.
4. bactericidal composition according to claim 1, it is characterised in that: also include filler and/or surfactant.
5. bactericidal composition according to claim 1 is for preventing and treating cereal, bean, fruit tree and fruits, vegetable Vegetables, root vegetables, forage grass class, turfgrass or turf, spice crop, on flower plant fungi and bacterium purposes.
6. bactericidal composition according to claim 1 be used to prevent and treat brown spot on apple, spot defoliation, ring spot, Anthracnose, shot hole；Powdery mildew, the gray mold of more crops；Rice sheath blight disease；Cabbage Leaf Spot；Lawn giant spot disease；Wheat leaf rust Disease；Wildfire；Chinese yam root rot；The purposes of downy mildew of garpe.
7. bactericidal composition according to claim 1 is used to handle seed to protect seed from fungi and bacteria attack Purposes.
8. bactericidal composition according to claim 1 is used to handle the soil of plant growth to protect the plants from soil Fungi and bacteria attack purposes.
9. a kind of method for preventing and treating plant pathogen, it is characterised in that: will power before plant is by disease infestation or after infecting Benefit require 1 described in bactericidal composition act on plant, plant propagation material and the plant organ then grown, cultivation medium, In material or space.
10. according to the method described in claim 9, it is characterized by: bactericidal composition described in claim 1 is had with agronomy The amount of application of effect and basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instiled, be poured, spray, sprayed The methods of mist, dusting, distribution or smoke be administered to plant, plant propagation material and the plant organ then grown, cultivation medium, In material or space.
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CN113265385A (en) * 2021-05-25 2021-08-17 西南大学 Broussonetia papyrifera antibacterial protein BpChiI, recombinant expression vector thereof and application of Broussonetia papyrifera antibacterial protein BpChiI in improving verticillium wilt resistance of plants
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Application publication date: 20191029