CN103988844B - A kind of bactericidal composition - Google Patents
A kind of bactericidal composition Download PDFInfo
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- CN103988844B CN103988844B CN201410234978.4A CN201410234978A CN103988844B CN 103988844 B CN103988844 B CN 103988844B CN 201410234978 A CN201410234978 A CN 201410234978A CN 103988844 B CN103988844 B CN 103988844B
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Abstract
The present invention relates to a kind of bactericidal composition, comprise bellkute and CGA-173506 active component. A kind of method that the invention still further relates to controlling plant diseases. The invention still further relates to a kind of method preventing and treating fruit vegetables storing period disease. A kind of bactericidal composition, comprises bellkute and CGA-173506 active component, and the weight ratio of described bellkute and CGA-173506 is 100:1-1:100. The content of bellkute and CGA-173506 active component accounts for the 5%-90% of compositions. The present invention, by bellkute and CGA-173506 are carried out binary built, acts on plant, plant propagation material and the organ grown subsequently, environment to prevent and treat the fungus on frumentum, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant, has reached the effect of potentiation. And the bactericidal composition containing bellkute and CGA-173506 can be used for processing the seed needing plant to be protected, it may also be used for process the fruit and vegerable of storage period after gathering in the crops.
Description
Technical field
The present invention relates to a kind of bactericidal composition, particularly relate to a kind of bactericidal composition in a kind of agricultural plant chemoproection field, said composition shows extremely strong antibacterial and bactericidal activity to crops or garden crop harmful fungoid. The invention still further relates to a kind of method preventing and treating plant pathogen. The invention still further relates to and control or the disease in storage after preventing and treating results fruit and vegerable results.
Background technology
Antifungal is natural or the compound in synthesis source, its infringement not caused by fungus for protecting plant. Current agricultural methods depend critically upon use antifungal. It practice, some crops can not grow effectively when not using antifungal. Use antifungal to make grower increase the quality of yield and crop, and thus increase the value of crop. In most cases, crop is worth at least three times that value added is the cost using antifungal.
But it does not have a kind of antifungal is all useful in all cases, and reuses single antifungal and frequently result in this kind or relevant antifungal are produced drug resistance. Therefore, the antifungal researching and producing more safely, there is better performance, needed relatively low dosage, easily use and expense relatively low and the compositions of antifungal.
Bellkute (Bellkute), double; two flesh octylame classes, mainly biosynthesis and cell membrane function to the class ester compounds of fungus work, it is suppressed that the formation of spore germination, germ tube elongation, appresorium and mycelia, are that a class is tagged and preventative sterilization. Mainly prevent and treat the fungal disease caused by most of ascomycetess and Fungi Imperfecti. ?end row Х happy position chin or cheek altogether ? to chase after Juan's 14. ? wink 14. タ Xian 18. ? Xian (8) roasting slash of also 18. controlling 10. carefully to send a (14) porphin ? and kowtow muddled the Huangshui River and fear the 9. figured stone ? such as ? and (3) belch altogether 18. that hall S altogether is around the salty crow chin or cheek of garden, the Wei dizzy ? of ? altogether. ? also ? Tartar kowtows muddled the Huangshui River and fears. ? table tennis practise chase after ? Xian under Juan's (10) saddle and bridle a horse [? rush down rhizome of chuanxiong rose nurse man А 5 ? moat ヒ bad nurse happy stomach U.S. whiz " a Γ ? ? ≡ ? an ancient unit of weight equal to 20 or 24 *taels of silver again vast scheme the Huangshui River soot moral erosion Xi ? that falls from the sky or outer space leads ≡ ? iron chains rooms at the sides of or facing the principal rooms ┮ ┘ altogether and holds back ? ? wink bright ? pool magpie order and hang the abundant ? of ┬ and (3) move river in Henan Province and hang ┮ glutinous ? ? and control the ā 0 suddenly stupid boundless and indistinct ? ? ? ? Pi ? that discusses; 1 one imino groups (octyl group methylene) biguanide 3 (alkyl benzene sulfonate), and its chemical structural formula is as follows:
N=10～13
CGA-173506 (Fludioxonil), chemical name: 4-(2,2-bis-fluoro-1,3-benzo dioxy-4-base) pyrroles's-3-nitrile or 4-(2,2-bis-fluoro-1,3-benzodioxolane-4-base) pyrroles's-3-nitrile. Chemical constitution is:
CGA-173506 is pyroles antibacterial, known by EP-A-206999. The contact antibacterial of CGA-173506, for seed treatment, can prevent and treat seed-borne fungi and soilborne fungal disease. Lasting period is long, and not easily with other antibacterial generation cross resistance.
CGA-173506 is used for Semen Tritici aestivi, Fructus Hordei Vulgaris, Semen Maydis, Semen Pisi sativi, Brassica campestris L, Oryza sativa L., vegetable, Fructus Vitis viniferae, lawn, ornamental crops foliar treatment, preventing and treating snow rotten Fusariumsp, Semen Tritici aestivi net raw meat black rot, rhizoctonia solani, and gray mold is had specially good effect; Corn and non-cereal seed are processed, and preventing and treating kind passes and soil-borne disease bacterium, such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, Rhizoctonia and Penicillium bacterium. When seed germination, CGA-173506 can be absorbed on a small quantity, such that it is able to the pathogenic bacteria controlled in seed and caryopsis; CGA-173506 moves hardly in soil simultaneously, therefore, it is possible to the region being maintained in around seed, provides long-term protection to crop root. CGA-173506 is one of whole world product obtaining EPA EPA " low-risk " certification few in number.
Summary of the invention
The present invention seeks to the defect for above-mentioned technology, a kind of bactericidal composition, it is to provide harmful fungoid is had and improves the compositions (Synergistic compositions) that total amount of application of activity and reactive compound reduces, to reduce rate of application and to expand the activity profile of known compound.
The present invention, through various further investigations, found that the bactericidal composition containing bellkute and CGA-173506, can prevent and treat the fungal disease of vegetable, fruit tree and cereal crop. Compositions containing bellkute and CGA-173506 is not only for common phytopathogen, and the pathogen that those have produced drug resistance is also highly effective, also has excellent prevention effect, thus complete the present invention under disease recurs condition.
We have found that and use simultaneously, namely use or use respectively bellkute and CGA-173506 together, or use bellkute successively and harmful fungoid preventive and therapeutic effect that CGA-173506 provides is better than the effect individually using each compound. In other words, there is cooperative effect unpredictable, necessary being, and be not only activity add and.
When reactive compound is present in the bactericidal composition of the present invention with specific weight ratio, cooperative effect is obvious especially. But, the weight ratio of the reactive compound in bactericidal composition can change within the specific limits.
One bactericidal composition of the present invention is to take techniques below scheme to realize:
One bactericidal composition of the present invention, it is characterised in that: comprise bellkute and CGA-173506, wherein the weight proportion 1:100-100:1 of bellkute and CGA-173506, it is preferable that 1:50-50:1, more preferably 1:25-25:1, more preferably 1:10-10:1.
A kind of bactericidal composition, comprises bellkute and the active component of CGA-173506, filler and/or surfactant.
Described bactericidal composition dosage form is wettable powder, cream, suspending agent, suspension emulsion, microcapsule, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foam, unguentum, aerosol, ultra-low volume spray liquid.
Described bactericidal composition, wherein accounts for the 5%-90% of bactericidal composition containing the content of bellkute and the active component of CGA-173506.
Described bactericidal composition, wherein accounts for the 10%-80% of bactericidal composition containing the content of bellkute and the active component of CGA-173506.
Described bactericidal composition, wherein accounts for the 20%-60% of bactericidal composition containing the content of bellkute and the active component of CGA-173506.
A kind of method preventing and treating plant pathogen, acts on plant, plant propagation material and the organ grown subsequently, environment by described bactericidal composition.
Described bactericidal composition processes the propagating materials needing plant to be protected.
The plant propagation material that described bactericidal composition processes.
Described bactericidal composition is for preventing and treating the purposes of fungus.
Described bactericidal composition is for preventing and treating the purposes of fungus on Cereal, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant.
Described bactericidal composition is used for preventing and treating gray mold, sclerotiniose, brown rot, leaf spot, banded sclerotial blight, full rot, rust, anthrax, early blight, powdery mildew, scab, melasma, blight dis-ease, spot defoliation, stem wilt etc.
Described bactericidal composition is for preventing and treating the purposes of fruit vegetables storing period disease.
A kind of method preventing and treating plant pathogen, uses bellkute and CGA-173506 simultaneously or uses respectively or sequential application.
A kind of method protecting seed, it includes making seed prior to seeding and/or contacting with described bactericidal composition after accelerating germination.
Character according to these compositionss and use purpose and the ambient conditions that compositions to reach, it is possible to select compositions with coating, spraying, immersion, dense fog, the mode such as broadcast sowing or pour and use.
Detailed Description Of The Invention
The synergy of bellkute and CGA-173506, the weight ratio of reactive compound can select in relatively larger scope. The weight ratio of bellkute and CGA-173506 is 100:1-1:100, or 50:1-1:50, or 20:1-1:20, it is preferable that 10:1-1:10, more preferably 5:1-1:5.
The bactericidal composition of the present invention, the content of active component depends on the dosage sterilized when being used alone, and also depends on mixed ratio and the potentiation degree of a kind of antibacterial and another kind of antibacterial.
In described bactericidal composition, the content of bellkute and CGA-173506 active component accounts for the 5%-90% of bactericidal composition, it is preferable that 10%-80%, more preferably 20%-60%.
In described bactericidal composition, it is possible to only comprise active component, it is also possible to comprise filler and/or surfactant.
The bactericidal composition of the present invention, it is possible to prepare into various dosage form, for instance wettable powder, cream, suspending agent, suspension emulsion, microcapsule, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foam, unguentum, aerosol, ultra-low volume spray liquid.
According to the present invention, term " filler " refers to can be combined with reactive compound or combine to make it easier for be administered to the naturally occurring or synthetic organic or inorganic compound of object (such as plant, crop or grass class). Therefore, described filler is preferably inertia, at least should be agricultural acceptable. Described filler can be solid or liquid.
Liquid filler is generally: water, alcohol type (such as methanol, ethanol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, Ketohexamethylene etc.), ethers (such as ether, dioxanes, methylcellulose, oxolane etc.), aliphatic hydrocarbon class (such as kerosene, mineral wet goods), aromatic hydrocarbons class (such as benzene, toluene, dimethylbenzene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbon, chlorobenzene, Deng), halogenated hydrocarbon class, amide-type, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal wet goods.
Solid-filling agent is generally: phyteral powdery type (the such as granule of Semen sojae atricolor powder, starch, grain dust, wood powder, bark powder, sawdust, walnut shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem, extract the residue etc. after plant essence), paper, sawdust, pulverize the synthesized polymer body of synthetic resin etc., clay class (such as Kaolin, Bentonite, acid china clay etc.), Pulvis Talci class. Silica (such as kieselguhr, silica sand, Muscovitum, moisture silicic acid, calcium silicates), activated carbon, natural mineral matter class (Pumex, attapulgite and zeolite etc.), fire the chemical fertilizer of the inorganic mineral powder of kieselguhr, sand, plastics medium etc. (such as polyethylene, polypropylene, polyvinylidene chloride etc.), potassium chloride, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, carbamide, greening ammonium etc., clay fertilizer, these materials can be used alone or two or more is used with.
For making effective ingredient compound emulsifying, dispersion, solubilized, and/or moistening can use surfactant such as can enumerate fatty alcohol-polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, the phosphate ester of polyoxyethylene alcohol or phenol, the fatty acid ester of polyhydric alcohol, alkyl aryl sulphonic acid, naphthalene sulfonic acid polymer, lignosulfonates, a shape copolymer of macromolecule comb shape, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, oils and fats, fatty alcohol and ethylene oxide condensate, the polyacrylates such as alkyltaurate, protein hydrolysate. suitable oligosaccharide thing or polymer, for instance based on the combination of independent vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine.
Preparation it be also possible to use viscosifier such as carboxymethyl cellulose and the natural and synthetic polymer of powder, granule or latex form, for instance Radix Acaciae senegalis, polyvinyl alcohol and polyvinyl acetate or natural phospholipid, for instance cephalin and lecithin, and synthetic phospholipid. Other additive is mineral oil and vegetable oil.
It is likely to the coloring agent such as inorganic pigment used, for instance, ferrum oxide, titanium oxide and Prussian blue, and organic dyestuff, such as alizarin dyes, azo dye or metal phthalein mountain valley with clumps of trees and bamboo dyestuff, and trace nutrient, such as ferrum, manganese, boron, ketone, cobalt, molybdenum and zinc salt.
The disintegrating agent being likely to use is selected from: one or more in bentonite, carbamide, ammonium sulfate, aluminum chloride, citric acid, succinic acid, sodium bicarbonate.
The stabilizer being likely to use is selected from: the one in sodium citrate, resorcinol.
The antifreezing agent being likely to use is selected from: one or more in ethylene glycol, propylene glycol, glycerol, carbamide.
Described defoamer is selected from: silicone oil, silicone compound, C10-20Saturated fat acid compounds, C8-10One or more in aliphatic alcohols compound.
Optionally, other annexing ingredient can also be comprised, for instance protecting colloid, binding agent, thickening agent, thixotropic agent, penetrating agent, stabilizer, screening agent.
Described active component can be mixed with filler and/or surfactant and prepare by known way by the described preparation of the present invention, also can add other conventional additives, such as drier and coloring agent, stabilizer, pigment, defoamer, preservative, thickening agent etc.
The bactericidal composition of the present invention with other medicament with weeding, parasite killing or bactericidal property, particularly can also mix use, it is also possible to mix use with insecticide, protective agent, growth regulator, nutrient for plants or soil conditioner with protective fungicide.
The bactericidal composition of the present invention has extraordinary fungicide performance, and can be used for preventing and treating phytopathogenic fungi, described fungus is especially selected from Ascomycetes (Ascomycetes), Basidiomycetes (Basidiomycetes), Phycomycetes (Phycomycetes) and deuteromycetes (Deuteromycetes) fungus.
The bactericidal composition of the present invention is particularly suited for preventing and treating following plant disease:
Rod method (AIternaria) on-vegetable, oilseed rape, sugar beet, fruit and rice belongs to,
Silk capsule mould (Aphanomyces) on-sugar beet and vegetable belongs to,
Bipolaris (Bipolaris) in-Semen Maydis, Cereal, rice and lawn belongs to and the compacted spore of interior umbilicus (Drechslera) belongs to,
Standing grain powdery mildew (Blumeriagraminis) (powdery mildew) on-Cereal,
Botrytis cinerea (Botrytiscinerea) (gray mold) on-Fructus Fragariae Ananssae, vegetable, flowers and vine,
Caulis et Folium Lactucae sativae dish on-Caulis et Folium Lactucae sativae obstructs mould (Bremialactucae),
Tail spore (Cercospora) on corn and soybean, rice and sugar beet belongs to,
Cochliobolus (Cochliobolus) on-Semen Maydis, Cereal, rice belongs to (the standing grain rotation spore on such as Cereal
Chamber bacterium (Cochliobolussativus), palace portion cochliobolus (Cochliobolusmiyabeanus) on rice),
Thorn dish spore (Colletotricum) on-Semen sojae atricolor and Cotton Gossypii belongs to,
The compacted spore of interior umbilicus (Drechslera) on-Cereal and Semen Maydis belongs to,
The compacted spore of prominent umbilicus (Exserohilum) on-Semen Maydis belongs to,
. two spore powdery mildews (Erysiphecichoracearum) on Fructus Cucumidis sativi and monofilament shell powdery mildew
(Sphaerothecafuliginea),
Fusarium (Fusarium) in-each kind of plant belongs to and wheel branch spore (Verticillium) belongs to,
Gaeumannomyce (Gaeumanomycesgraminis) on-Cereal belongs to,
Red mould (Gibberella) on-Cereal and rice belongs to the (gibberella fujikuroi (Gibberella on such as rice
fujikuroi)),
Gram’s staining coordination compound (Grainstainingcomplex) on-rice,
The compacted spore of length (Helminthosporium) on-Semen Maydis and rice belongs to,
Michrodochiumnivale on-Cereal,
Spherical cavity bacterium (Mycosphaerella) on-Cereal, Fructus Musae and Semen arachidis hypogaeae belongs to,
Phakopsora pachyrhizi (Phakopsorapachyrhizi) on-Semen sojae atricolor and mountain horse sulphur layer rest fungus
(Phakopsarameibomiae),
Phomopsis (Phomopsis) on-Semen sojae atricolor and Helianthi belongs to,
Phytophthora infestans (Phytophthorainfestans) on-Rhizoma Solani tuber osi and Fructus Lycopersici esculenti,
The raw single shaft of Fructus Vitis viniferae on-vine mould (Plasmoparaviticola),
Apple mildew bacterium (Podosphaeraleucotricha) on-Fructus Mali pumilae,
Semen Tritici aestivi Phyllostachys pubescens (Pseudocercosporellaherpotrichoides) on-Cereal,
False downy mildew (Pseudoperonospora) on-Flos lupuli (Flos Humuli Lupuli) and calabash belongs to,
Handle rest fungus (Puccinia) on-Cereal and Semen Maydis belongs to,
Nuclear cavity bacteria (Pyrenophora) on-Cereal belongs to,
Pyricularia oryzae (Pyricularia0 η zae) on-rice, wood photovoltaicing leather bacteria (Corticiumsasakii), broom stalk Cylindrocarpon (Sarocladiumoryzae), sheath rot of rice plant (S.attenuatum), rice leaf smut (Entylomaoryzae)
Piricularia oryzae (Pyriculariagrisea) on-lawn and Cereal,
-lawn, rice, Semen Maydis, Cotton Gossypii, oilseed rape, Helianthi, sugar beet, vegetable and other plant
Corruption mould (Pythium) on thing belongs to,
-Cotton Gossypii, rice, Rhizoma Solani tuber osi, lawn, Semen Maydis, oilseed rape, Rhizoma Solani tuber osi, sugar beet, vegetable and its
Rhizoctonia (Rhizoctonia) on his plant belongs to,
Sclerotinite (Sclerotinia) in-oilseed rape and Helianthi belongs to,
Wheat septoria (Septoriatritici) on-Semen Tritici aestivi and the many spore (Stagonospora of the withered shell of grain husk
nodorum),
Fructus Vitis viniferae snag shell (Erysiphe) on-vine,
Setospaeria on-Semen Maydis and lawn belongs to,
Silk axle smut (Sphacelothecareilinia) on-Semen Maydis,
Thielaviopsis sp (Thievaliopsis) on-Semen sojae atricolor and Cotton Gossypii belongs to,
Tilletia foetida (Tilletia) on-Cereal belongs to,
Smut (Ustilago) on-Cereal, Semen Maydis and sugar beet belongs to,
Black star bacterium (Venturia) on-Fructus Mali pumilae and pears belongs to (scab).
The bactericidal composition of the present invention is even more important to preventing and treating a large amount of fungus in various crops such as Cotton Gossypii, vegetable variety (such as Fructus Cucumidis sativi, beans, Fructus Lycopersici esculenti, Rhizoma Solani tuber osi and cucurbitaceous plant), Fructus Hordei Vulgaris, dogstail, Herba bromi japonici, Fructus Musae, coffee, Semen Maydis, fruit variety, rice, rye (Secale cereale L.), Semen sojae atricolor, vine, Semen Tritici aestivi, ornamental plant, Semen arachidis hypogaeae, Brassica campestris L, Caulis Sacchari sinensis and various crop seed.
The bactericidal composition of the present invention is particularly suitable for preventing and treating following plants pathogenic epiphyte: the standing grain powdery mildew (Blumeriagraminis) (powdery mildew) in Cereal, two spore powdery mildews (Erysiphecichoracearum) in cucurbitaceous plant and monofilament shell (Sphaerothecafuliginea), apple mildew bacterium (Podosphaeraleucotricha) in Fructus Mali pumilae, Fructus Vitis viniferae snag shell (Uncinulanecator) in vine, handle rest fungus (Puccinia) in Cereal belongs to, Cotton Gossypii, rhizoctonia (Rhizoctonia) in rice and lawn belongs to, smut (Ustilago) in Cereal and Caulis Sacchari sinensis belongs to, venturia inaequalis (Venturiainaequalis) (scab) in Fructus Mali pumilae, the compacted spore of length (Helminthosporium) in Cereal belongs to, wheat glume blotch bacterium (Septorianodorum) in Semen Tritici aestivi, Fructus Fragariae Ananssae, vegetable, Botrytis cinerea (Botrytiscinerea) (gray mold) in ornamental plant and vine, Semen arachidis hypogaeae tail spore (Cercosporaarachidicola) in Semen arachidis hypogaeae, eye spot bacterium (PseudocercosporellaHerpotrichoides) in Semen Tritici aestivi and Fructus Hordei Vulgaris, Pyricularia oryzae (Pyriculariaoryzae) in rice, phytophthora infestans (Phytophthorainfestans) in Rhizoma Solani tuber osi and Fructus Lycopersici esculenti, Fructus Vitis viniferae single shaft in vine is mould (Plasmoparaviticola), false downy mildew (Pseudoperonospora) in Flos lupuli (Flos Humuli Lupuli) and Fructus Cucumidis sativi belongs to, sclerotiniose (sclerotiose) in Brassica campestris L, rod method (Alternaria) in vegetable and fruit belongs to, spherical cavity bacterium (Mycosphaerella) in Fructus Musae belongs to and Neurospora (Fusarium) and wheel branch spore (Verticillium) genus.
Preferably, the bactericidal composition of the present invention can be used for preventing and treating on Cereal, water fruits and vegetables, Cotton Gossypii, fruit tree, ornamental plant gray mold, sclerotiniose, brown rot, leaf spot, banded sclerotial blight, full rot, rust, anthrax, early blight, powdery mildew, scab, melasma, blight dis-ease, spot defoliation, stem wilt etc.
The bactericidal composition of the present invention can be additionally used in preventing and treating fruit vegetables storing period disease. The fruit rot such as caused by following pathogen:
Aspergillus bacterium, for instance Aspergillus flavus;
Botrytis (Botrytis) strain, for instance Botrytis cinerea (Botrytiscinerea);
Penicillium (Penicillium) strain, for instance penicillium expansum (Penicilliumexpansum) and P.purpurogenum;
Sclerotinia (Sclerotinia) strain, for instance sclerotinite (Sclerotiniasclerotiorum);
Verticillium (Verticilium) strain, for instance black and white wheel branch spore (Verticiliumalboatrum).
The bactericidal composition of the present invention can be additionally used in preventing and treating kind and passes and soil-borne disease. The kind such as caused by following pathogen passes the rotten and wilting disease and seedling disease that pass with soil:
Alternaria belongs to plants, for instance the raw rod method (Alternariabrassicicola) of Caulis et Folium Brassicae campestris;
Aphanomyces (Aphanomyce) belongs to plants, for instance the mould (Aphanomyces of Kidney bean silk capsule
euteiches);
Ascochyta (Ascochyta) belongs to be planted, for instance Ascochytalentis;
Aspergillus, for instance Aspergillus flavus;
Cladosporium belongs to plants, for instance draft branch spore (Cladosporiumherbarum);
Cochliobolus belongs to plants, for instance standing grain cochliobolus;
(conidial form: Drechslera, Syn: long compacted spore bacterium);
Colletotrichum belongs to be planted, for instance hair core anthrax (Colletotrichumcoccodes);
Fusarium belongs to plants, for instance yellow sickle spore;
Gibberella belongs to plants, for instance beautiful another name for Sichuan Province multitude is red mould;
Shell ball spore belongs to (Macrophomina) genus kind, for instance Kidney bean shell ball spore (Macrophomina
phaseolina);
Little setting-out shell belongs to strain, for instance avenge rotten little setting-out shell;
Penicillium belongs to plants, for instance penicillium expansum;
Phaeosphaeria strain, for instance Phaeosphaerianodorum;
Phoma (Phoma) belongs to plants, for instance black embryo Phoma sp (Phomalingam);
Phomopsis (Phomopsis), for instance Semen sojae atricolor Phomopsis (Phomopsissojae);
Phytophthora belongs to plants, for instance Phytophthora cactorum (Phytophthoracactorum);
Nuclear cavity Pseudomonas belongs to be planted, for instance wheat and barley nuclear cavity bacteria (Pyrenophoragraminea);
Pyricularia Sacc. (Pyricularia) belongs to plants, for instance Magnaporthe grisea (Pyriculariaoryzae);
Pythium kind, for instance Pythium ultimum;
Rhizoctonia belongs to plants, for instance Rhizoctonia solani Kuhn;
Rhizopus (Rhizopus) belongs to plants, for instance Rhizopus oryzae (Rhizopusoryzae);
Bulbil belongs to (Sclerotium) genus kind, for instance Sclerotium rolfsii (Sclerotiumrolfsii);
Core coral Pseudomonas (Typhula) belongs to plants, for instance meat spore core coral bacterium (Typhulaincarnata);
Verticillium (Verticillium) belongs to plants, for instance verticillium dahliae (Verticilliumdahliae);
The bactericidal composition of the present invention, under preventing and treating phytopathogenic fungi desired concn, the compatibility having had with plant.Therefore, plant, plant propagation material and the plant organ grown subsequently, environment, habitat or storage areas can be applied to during the bactericidal composition of the present invention.
Term " plant propagation material " is interpreted as referring to all plant parts having fertility, for instance seed, and it can be used for breeding the latter, and vegetable matter such as cuttage bar or tuber (such as Rhizoma Solani tuber osi). Therefore, plant part used herein includes plant propagation material. It is mentioned that such as seed (in the narrow sense), root, fruit, tuber, bulb, rhizome and plant part. The germination plant suppressed after germinateing from soil or after emerging and effective plant be also it can be mentioned that. Young plant can be undertaken all by dipping before transplantation or Local treatment is protected.
The plant organ grown subsequently is any part of the plant produced by plant propagation material such as seed. Plant part, plant organ and plant can also benefit from by active agent combinations or compositions are applied to plant propagation material protection plant avoid plant disease. Certain plants part and some plant organ grown subsequently can also regard plant propagation material as, himself can use (or process) with bactericidal composition; Thus the plant produced by treated plant part and treated plant organ, other plant part and other plant organ can also be benefited from avoids plant disease by bactericidal composition is applied to certain plants part and certain plants Organoprotective plant.
According to the present invention, the process that plant is carried out by the bactericidal composition of the present invention is used to be made directly according to conventional treatment method, or realize by processing its local environment, habitat or storage space, described conventional treatment method such as immersion, spraying, evaporation, atomizing, broadcast sowing, smear; For plant propagation material or the plant organ that grows subsequently, especially seed, one or more layers coating also can be carried out. Type of service depends on desired purpose; Under any circumstance all it is ensured that the present composition as far as possible finely and is evenly distributed. Preferred application process is to be administered to aboveground vegetation part, especially foliage applying; Application times and use ratio and depend on biology and the weather conditions of existence of objective microbe. Active component can also reach in plant by root system via soil or water, and thus available liquid preparation waters location or this material is mixed in soil in solid form. The bactericidal composition of the present invention can also be administered on seed-grain in seed treatment, and thus tuber or corn can soak successively with the liquid preparation of every kind of active component, or wraps up with blended wet or dry preparation.
The bactericidal composition of the present invention is suitable for propagating materials such as seed, fruit, tuber or the kernel or plant cutting of protection plant and is not encroached on by pathogenic bacteria. Can before administration with this propagating materials of compositions-treated, for instance dress seed prior to seeding. Also by soaking kernel in fluid composition or being coated kernel with solid composite, active component is applied on kernel (being coated). When propagating materials being applied to that such as seed is broadcast in ditch in seeding process, it is possible to said composition is applied to application site. Processing method and the plant propagation material being processed as of plant propagation material are all further subject matters of the present invention by these.
In order to process plant propagation material particularly seed, also by impregnating tuber or grain in succession with the liquid preparation of each active component, or by with the wet or dry preparation coating combined, thus bellkute and CGA-173506 being administered on seed (coating).
A kind of method protecting seed, it includes making seed prior to seeding and/or contacting with the bellkute of cooperative effective quantity and the bactericidal composition of CGA-173506 after accelerating germination.
A kind of seed of the bactericidal composition scribbled or contain bellkute and CGA-173506.
The seed treatment of the present invention use by prior to seeding and/or after accelerating germination by seed spraying or dusting are carried out.
To damaging the preventing and treating of plant disease of plant after Seedling mainly through carrying out with crop protection compositions-treated soil and aboveground vegetation part. It is contemplated that environment and human and animal's health are likely to the impact brought by crop production compositions, so making great efforts to reduce the amount of used reactive compound.
The sterilization compound of the present invention is applied to seed with itself or suitable dosage form. Preferably, seed is processed at steady state, does not cause any infringement so that process. The process of seed generally can be carried out any time between gathering and sowing.
Generally it has to be noticed that the amount of the amount and/or other additive that are applied to the present composition of seed is chosen as the germination without influence on seed or without compromising on gained plant during seed treatment.
The bactericidal composition of the present invention for process plant propagation material, particularly Oryza sativa L., Brassica campestris L, Cotton Gossypii, Semen Tritici aestivi, Fructus Hordei Vulgaris, Semen sojae atricolor, Semen Maydis, Semen arachidis hypogaeae, Citrullus vulgaris, Helianthi seed be particularly advantageous.
The environment of plant growing, habitat refer to the supporter making crops take root, growing, such as: soil, water etc., concrete raw material can use such as sand, Pumex, Vermiculitum, kieselguhr, agar, gelling material, polymer substance, asbestos, wood flour, bark etc. Preferred soil.
Soil pathogenic bacteria has phytophthora, pythium spp, botrytis cinerea, soft-rot fungi. In the ordinary course of things, soil pathogenic bacteria can produce a large amount of thalline, as long as pathogen growth is grown favourable by condition, host is susceptible, pathogenic bacteria just can amount reproduction can infect host, under susceptible host exists, these pathogenic bacterias just can enter the lasting pathogenic phase, and along with the continuous cropping of crop, amount reproduction spreads, but afterwards nutrient be depleted or edaphic condition such as temperature, humidity to pathogenic bacteria unfavorable time, pathogenic bacteria can enter again rest period. When susceptible host is absent from, soil-borne disease bacterium also can survive in soil, has widely except host range except soil pathogenic bacteria, moreover it is possible to survive on the root surface or the broken branches and fallen leaves of non-host, have saprophytic competitiveness with it and be undivided.
Effective ingredient mixes environment or the habitat at plant place, for instance mixing in the form of granules (soil application) in soil, effective ingredient enters inside plants also by root system. The method using medicament in soil, such as liquid preparation it is diluted in water or does not dilute the medium method in rice seedling bed of root or the seedling being directly applied to plant, the method being disseminated to by granule in the root of plant or the rice seedling bed of seedling has the method being sprayed in soil by powder, water dispersible granules etc. prior to seeding and mixing with soil entirety, it is sprayed in implantation hole, seed furrow after powder, water-dispersible grain dilution agent before sowing or before serike body, then carries out the method etc. sowed.
In the preventing and treating of plant disease, bellkute and CGA-173506 separately or the using by the spraying of seed, plant or soil or dusting being carried out before or after plant seeding or before or after plant emergence of the compositions of co-administered or bellkute and CGA-173506. The compositions of the present invention can be used before or after plant or plant propagation material are by infection process.
Product of the present invention can finished product preparation form provide, and namely in bactericidal composition, each material is blended, and the composition of bactericidal composition can also provide by unit dose form, directly directly mixes in bucket or in tank, be then diluted to required concentration before using.The frequency of administration of bactericidal composition of the present invention and amount of application, according to the mixing ratio of effective ingredient, meteorological condition, dosage form, Dressing date, application process, use place, prevent and kill off the difference of object noxious livings, objective crop etc. and variant:
-generally for leaf portion process active substance concentration be: 10-600g/ha, it is preferable that 30-500g/ha, more preferably 50-300g/ha; For using for dipping or instillation, described dosage even can also reduce, particularly when using inert base such as asbestos or ZHENZHUYANSHI;
-for seed treatment: 1-6000g active substance/100kg seed, it is preferable that 5-5000g active substance/100kg seed;
-for soil treatment: 10-6000g/ha, it is preferable that 50-3000g/ha;
-for fruit and vegerable impregnation process: 200-2000 times of liquid of dilute with water, leaching fruit one minute.
Above-mentioned dosage is only general exemplary dose, actual when using those skilled in the art according to practical situation and needs, rate of application can be adjusted especially in accordance with the character of pending plant or fruit and vegerable and the seriousness of pathogenic bacteria.
The bactericidal composition of the present invention can be used by common method, and amount of application changes with weather or crop condition, use optimum period be crop susceptible before or the susceptible initial stage. Its lasting period is generally relevant with the content in bactericidal composition, also relevant to extraneous factor.
The bactericidal composition of the present invention has the advantage that
A kind of bactericidal composition, is to provide harmful fungoid is had and improves the compositions (Synergistic compositions) that total amount of application of activity and reactive compound reduces, to reduce rate of application and to expand the activity profile of known compound.
The present invention contains bellkute and the bactericidal composition of CGA-173506 active component, can prevent and treat the fungal disease of vegetable, fruit tree and cereal crop. Compositions containing bellkute and CGA-173506 is not only for common phytopathogen, and the pathogen that those have produced drug resistance is also highly effective, also has excellent prevention effect under disease recurs condition.
One bactericidal composition of the present invention contains bellkute and CGA-173506 active component is used simultaneously, namely use or use respectively bellkute and CGA-173506 together, or use bellkute successively and harmful fungoid preventive and therapeutic effect that CGA-173506 provides is better than the effect individually using each compound. In other words, there is cooperative effect unpredictable, necessary being, and be not only activity add and.
When reactive compound is present in the bactericidal composition of the present invention with specific weight ratio, cooperative effect is obvious especially. But, the weight ratio of the reactive compound in bactericidal composition can change within the specific limits.
In order to make the purpose of the present invention, technical scheme and advantage clearly understand, present invention specific examples below illustrates, but the present invention is limited to absolutely not these examples. It should be pointed out that, all any amendment, equivalent replacement and improvement etc. made within the spirit and principles in the present invention, should be included within protection scope of the present invention.
Detailed description of the invention
Below with reference to embodiment, the invention will be further described:
Example of formulations
Embodiment 12% bellkute+10% CGA-173506 wettable powder
Bellkute 2%
CGA-173506 10%
Sodium lauryl sulphate 10%
Sodium lignin sulfonate 5%
Kaolin complements to 100%
Active component, various auxiliary agent and filler etc. are pressed the proportional components mixing of formula, after ultra-fine pulverizer disintegrating, namely obtains 2% bellkute+10% CGA-173506 wettable powder.
Embodiment 21% bellkute+50% CGA-173506 wettable powder
Bellkute 1%
CGA-173506 50%
Calcium dodecyl benzene sulfonate 1%
Sodium lignin sulfonate 2%
Attapulgite complements to 100%
Active component, various auxiliary agent and filler etc. are pressed the proportional components mixing of formula, after superfine grinding, namely obtains 1% bellkute+50% CGA-173506 wettable powder.
Embodiment 31% bellkute+4% CGA-173506 cream
Bellkute 1%
CGA-173506 4%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
N-Methyl pyrrolidone complements to 100%
Mentioned component is proportionally prepared, is uniformly mixing to obtain homogeneous phase.
Embodiment 45% bellkute+50% CGA-173506 wettable powder
Bellkute 5%
CGA-173506 50%
Sodium lauryl sulphate 10%
Sodium lignin sulfonate 5%
White carbon complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder.
Embodiment 52% bellkute+50% CGA-173506 water dispersible granules
Bellkute 2%
CGA-173506 50%
MODIFIED LIGNOSULPHONATE 5%
Sodium lauryl sulphate 5%
Carbamide 5%
Kaolin complements to 100%
By bellkute active component, dispersant, wetting agent, disintegrating agent and filler in the ratio mix homogeneously of formula, become wettable powder through comminution by gas stream; Add CGA-173506 mix homogeneously; Add a certain amount of water mixing extruding and make material. 2% bellkute+50% CGA-173506 water dispersible granules is obtained after drying screening.
Embodiment 60.5% bellkute+50% CGA-173506 suspension emulsion
Oil phase:
Bellkute 0.5%
Methyl oleate 10%
Ethoxylated castor oil 5%
Aqueous phase:
CGA-173506 50%
The sodium salt 1% of the LOMAR PWA EINECS 246-676-2-formaldehyde condensation products of sulfonation
Water complements to 100%
Bellkute is dissolved in methyl oleate, adds ethoxylated castor oil and obtain oil phase; The aqueous suspension agent of CGA-173506 will be obtained after ground to CGA-173506, the sodium salt of LOMAR PWA EINECS 246-676-2-formaldehyde condensation products of sulfonation, water and/or high speed shear according to formula; Under agitation oil phase is added aqueous phase and obtain suspension emulsion.
Embodiment 710% bellkute+10% CGA-173506 wettable powder
Bellkute 10%
CGA-173506 10%
Sodium lignin sulfonate 1%
Sodium lauryl sulfate 2%
The silicic acid 1% of high degree of dispersion
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder.
Embodiment 840% bellkute+50% CGA-173506 coated granule
Bellkute 40%
CGA-173506 50%
Polyethylene Glycol 3%
The silicic acid 1% of high degree of dispersion
Calcium carbonate complements to 100%
In a mixer, by levigate active component even spread to by the carrier of Polyethylene Glycol moistening. Dust-free coated granules agent can be obtained in this way.
Embodiment 950% bellkute+10% CGA-173506 wettable powder
Bellkute 50%
CGA-173506 10%
Sodium lauryl sulphate 1%
Sodium lignin sulfonate 1%
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder.
Embodiment 1020% bellkute+60% CGA-173506 extrusion granule
Bellkute 20%
CGA-173506 60%
Sodium lignin sulfonate 4%
Carboxymethyl cellulose 2%
Kaolin complements to 100%
Active component is mixed with auxiliary agent and grinds, compositions water-wet. Said composition is extruded, then dries in the air stream.
Embodiment 1150% bellkute+2% CGA-173506 suspension seed-coating agent
Bellkute 50%
CGA-173506 2%
Fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoesters disodium 10%
MODIFIED LIGNOSULPHONATE 5%
Xanthan gum 1%
Hydroxyethyl cellulose 1%
Glycerol 5%
PVP-K301%
Water complements to 100%
Seed coat agent is obtained after above-mentioned each component is mixed in proportion ground and/or high speed shear.
Embodiment 1250% bellkute+5% CGA-173506 microcapsule suspension-suspendinagent agent
ATLOXTM49134%
Citric acid 0.05%
Catalyst 0.1%
Water 10%
CGA-173506 5%
Polyphenyl polymethylene polyisocyanates 1.35%
SOLVESSOTM2005%
ATLOXTM491316%
Dispersant LFH0.3%
Defoamer 0.16%
Carbamide 4.4%
Bellkute 50%
Water complements to 100%
By polyphenyl polymethylene polyisocyanic acid (PAPI), bellkute, SOLVESSOTM200 oil phases formed add containing ATLOXTMIn the aqueous solution of 4913, form emulsion. Then heating insulation are 50oCatalyst reaction is added 2 hours under C. The microcapsule of bellkute is obtained after cooling.
ATLOXTM4913, dispersant LFH, defoamer, carbamide, CGA-173506 and water are mixed in proportion, ground and/or obtain after high speed shear uniformly, obtaining suspending agent.
The bellkute microcapsule obtained is added in the suspending agent of CGA-173506, be uniformly mixing to obtain 50% bellkute+5% CGA-173506 microcapsule suspension-suspendinagent agent.
Embodiment 1350% bellkute+1% CGA-173506 suspending agent
Bellkute 50%
CGA-173506 1%
By each components such as active component, dispersant, wetting agent and the water ratio mix homogeneously according to formula, ground and/or high speed shear, control particle diameter below 5 μm, obtain 50% bellkute+1% CGA-173506 suspending agent.
Embodiment 1450% bellkute+0.5% CGA-173506 cream
Bellkute 50%
CGA-173506 0.5%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
SOLVESSOTM100 complement to 100%
Above-mentioned each component being mixed, stirring is to obtaining transparent homogeneous phase.
Embodiment 1520% bellkute+30% CGA-173506 water dispersible granules
Bellkute 20%
CGA-173506 30%
MODIFIED LIGNOSULPHONATE 5%
Sodium lauryl sulphate 5%
Ammonium sulfate 2%
Kaolin complements to 100%
By bellkute, CGA-173506 active component, dispersant, wetting agent, disintegrating agent and filler in the ratio mix homogeneously of formula, become wettable powder through comminution by gas stream; Add a certain amount of water mixing extruding and make material. 20% bellkute+30% CGA-173506 water dispersible granules is obtained after drying screening.
Embodiment 165% bellkute+30% CGA-173506 wettable powder
Bellkute 5%
CGA-173506 30%
Sodium lauryl sulphate 1%
Sodium lignin sulfonate 1%
Kaolin complements to 100%
Said components is mixed in proportion, and grinds, pulverize, prepare into wettable powder.
Embodiment 171% bellkute+10% CGA-173506 aqueous emulsion
Aqueous phase:
Bellkute and CGA-173506 are dissolved in methyl oleate, add polystyrene and obtain oil phase; Aqueous phase is obtained according to the component mix homogeneously in formula; Under agitation oil phase is added aqueous phase and obtain aqueous emulsion.
Embodiment 185% bellkute+5% CGA-173506 microemulsion
Bellkute 5%
CGA-173506 5%
Ethoxylated castor oil 10%
Calcium dodecyl benzene sulfonate 10%
SOLVESSOTM10050%
Water complements to 100%
Above-mentioned each component being mixed, stirring is to obtaining transparent homogeneous phase.
Embodiment 1940% bellkute+60% CGA-173506
Bellkute 40%
CGA-173506 60%
By bellkute, CGA-173506 proportionally mix homogeneously.
Embodiment 2050% bellkute+50% CGA-173506
Bellkute 80%
CGA-173506 20%
Proportioning in above example is weight per distribution ratio.
Embodiment
In order to better explain the invention main points of the present invention, provide below several preferably embodiment.
The combination of active principles of difference pesticide is made pesticide, is develop and develop novel pesticide at present and prevent and treat a kind of effective and quick way of agriculturally resistance pathogenic bacteria. After the pestsides synthesis of different cultivars, typically exhibit three kinds of type of action: summation action, potentiation and antagonism. But it is specially which kind of effect, it is impossible to prediction, only just be can know that by great many of experiments. Compound synergic is good formula, and owing to significantly improving actual prevention effect, what reduce pesticide makes consumption, thus greatly having delayed the drug-fast generation speed of pathogenic bacteria, is the important means of integrated control disease.
The combination of bellkute and CGA-173506 is carried out effect analysis by the abundant method of Sun Yun, Colby formula by the present invention, it has been found that in certain ratio range, and obtained bactericidal composition has gain effect, and is not only the simple addition of two kinds of medicaments.
Toxicity Determination
Test 1: the toxicity test to grey mould fruit rot of strawberry
Adopt spore germination test method(s). Choosing the Strawberry seedlings that growing way is consistent, each process selects 3 basins for examination Strawberry seedlings, sprays under 50PSI pressure with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 Concentraton gradient. After chemicals treatment, 24h connects bacterium, the grey mould fruit rot of strawberry leaf picking up from field is uniformly shaken off conidium above seedling of Fructus Fragariae Ananssae and inoculates, and is then put into by Strawberry seedlings in greenhouse and cultivates. Investigate disease index according to the morbidity grade scale Herb of grey mould fruit rot of strawberry after 7d, and calculate prevention effect, then calculate concentration EC in suppressing with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.
Computational methods:
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100
The percentage composition of B in the percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI) * 100
CTC < 80 is antagonism, and 80≤CTC≤120 are summation action, and CTC > 120 is potentiation,
Table 1: the virulence test result to grey mould fruit rot of strawberry
As known from Table 1, when the combination preventing and treating graw mold of tomato of bellkute and CGA-173506 is in the scope of proportioning 100:1-1:100, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at the mixture within the scope of this.
Test 2: the toxicity test of sclerotinia rot of colza
Adopting spore germination test method(s): choose the Brassica campestris L seedling that growing way is consistent, each process selects 3 basins for examination seedling, sprays under 50PSI pressure with potter spray tower, and the about 5mL of every basin, each medicament arranges 12 Concentraton gradient. After chemicals treatment, 24h connects bacterium, the sclerotinia rot of colza leaf picking up from field is uniformly shaken off conidium above Brassica campestris L seedling and inoculates, and is then put into by Brassica campestris L seedling in greenhouse and cultivates. Investigate disease index according to the morbidity grade scale Herb of sclerotinia rot of colza after 7d, and calculate prevention effect, then calculate concentration EC in suppressing with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.
Table 2: the virulence test result to sclerotinia rot of colza
As known from Table 2, when the combination preventing and treating sclerotinia rot of colza of bellkute and CGA-173506 is in the scope of proportioning 100:1-1:100, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at the mixture within the scope of this.
Test 3: the toxicity test of Penicillium italicum
Adopting and suppress mycelial growth rate method: experiment target is designated as Penicillium italicum, test adopts co-toxicity coefficient method to evaluate the co-toxicity effect of complex preparation.
Bellkute and CGA-173506 are used acetone solution respectively, the medicinal liquid being configured to series concentration is diluted again with the tween 80 aqueous solution of 0.1%, superclean bench is drawn the 6mL conical flask to sterilizing respectively, add potato dextrose agar (PDA) 54mL of about 50 DEG C, pour the plate of 4 diameter 9cm after shaking up into, make the toxic culture medium of 4 respective concentration; Toxic culture medium is made by the same method bellkute by different ratio and CGA-173506 series concentration compound solution. By cultivating the Penicillium italicum bacterium of 2 days, break into truffle with the card punch of diameter 5mm at colony edge, with Inoculating needle, truffle is moved to the toxic PDA culture medium central authorities being configured in advance, is subsequently placed in 25 DEG C of incubators and cultivates, often process and repeat 4 times.After 3 days, adopt decussation method slide calliper rule to measure each process colony diameter cm, obtain correction inhibition percentage. Each bacterium colony decussation surveys two diameters, represents bacterium colony size with its average. Then colony growth suppression ratio is obtained by following formula:
Then concentration EC in suppressing is calculated with method of least square50, then calculate co-toxicity coefficient (CTC) according to the abundant method of Sun Yun.
Table 3: the virulence test result to Penicillium italicum
As known from Table 3, when the combination preventing and treating Penicillium italicum of bellkute and CGA-173506 is in the scope of proportioning 100:1-1:100, co-toxicity coefficient is all higher than 120, illustrates that both all show as gain effect at the mixture within the scope of this.
Biological test example
When the effect of active compound combinations exceedes the summation of the effect when each reactive compound is individually used, there is synergistic function. The predictive role of the particular combination of two kinds of reactive compounds can use so-called " Colby formula " (referring to S.R.Colby, " CalculatingSynergisticandAntagonisticResponsesofHerbicid eCombinations ", Weeds1967,15,20-22) calculated as below: if
X is the activity when using the reactive compound A that consumption is mg/ha or concentration is mppm;
Y is the activity when using the reactive compound B that consumption is ng/ha or concentration is nppm, is expressed as accounting for the percentage rate of untreated control;
E is the activity when using the reactive compound A and B that consumption is m and ng/ha or concentration is m and nppm,
So
If the activity of actual observation (O) is more than expection activity (E), then said composition is super add and, namely there is potentiation.
Following biological test example is in order to illustrate the present invention. But, the present invention is not limited to these embodiments.
Test 1 ash arrhizus bacteria test (Fructus Fragariae Ananssae)
Solvent: the N-methyl-pyrrolidon of 7 parts of weight
Emulsifying agent: the alkylaryl polyglycol ether of 2 parts of weight
In order to prepare suitable reactive compound, the combination of 1 part of reactive compound by weight or reactive compound is mixed with solvent and the emulsifying agent of above-mentioned amount, and is diluted with water to desired concn.
In order to test therapeutical active, seedling Botrytis cinerea bacterium spore is dusted. After inoculating 48 hours, the Strawberry seedlings active agent preparations of described using dosage is sprayed. Then test plant is placed in temperature about 20OTo promote the growth of grey mold scab in the greenhouse of C, air humidity about 80%. Carrying out effect assessment after inoculating 7 days, 0% to refer to effect suitable with what compare, and 100% refers to and do not observe and infect. Reactive compound, using dosage and result of the test are listed in the following table:
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates, shows obvious potentiation.
Test 2 sclerotiniose test (Brassica campestris L)/seed treatment
Reactive compound is used with dry seeds seed dressing. Said preparation can be prepared by being mixed with levigate mineral by the reactive compound of independent reactive compound or combination, and obtaining can in the equally distributed fine-powdered compositions of the surface of the seed.
During seed seed dressing, seed and seed seed dressing shake 3 minutes in the glass flask closed will be infected. Semen Brassicae campestris is seeded in standard soil 1 centimetre deep, in temperature about 18OC, the warm indoor cultivation of air humidity about 95%, every 15 h light day by day of seeds cultivation case.
After sowing 3 weeks, evaluating the disease of Brassica campestris L seedling, 0% to refer to effect suitable with what compare, and 100% refers to and do not observe and infect.Reactive compound, using dosage and result of the test are listed in the following table:
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates, shows obvious potentiation.
Test 3: to the effect of Botrytis cinerea on Fructus Mali pumilae
Fructus Mali pumilae bores 3 holes, in each hole, instills the test compositions of 50ul preparation. After dispenser 2 hours, the spore suspension liquid-transfering gun pipe of 50ul Botrytis cinerea is moved on on dispenser position. 20OHatch in cultivating chamber under C 5 days, it is determined that the area % of infected fruit. Relative to the disease rate calculating effect on untreated Fructus Mali pumilae.
Result of the test shows, observes activity and exceedes the activity that use " Colby " formula calculates, shows obvious potentiation.
Claims (15)
1. a bactericidal composition, it is characterised in that: active component is made up of bellkute and CGA-173506, and the weight ratio of described bellkute and CGA-173506 is 100:1-1:100.
2. compositions according to claim 1, it is characterised in that: the weight ratio of described bellkute and CGA-173506 is 50:1-1:50.
3. compositions according to claim 1, it is characterised in that: the weight ratio of described bellkute and CGA-173506 is 25:1-1:25.
4. compositions according to claim 1, it is characterised in that: the weight ratio of described bellkute and CGA-173506 is 10:1-1:10.
5. compositions according to claim 1, it is characterised in that: also comprise filler and/or surfactant.
6. compositions according to claim 1, it is characterised in that: the content of bellkute and CGA-173506 active component accounts for the 5%-90% of compositions.
7. compositions according to claim 1, it is characterised in that: the content of bellkute and CGA-173506 active component accounts for the 10%-80% of compositions.
8. compositions according to claim 1, it is characterised in that: the content of bellkute and CGA-173506 active component accounts for the 20%-60% of compositions.
9. compositions according to claim 1, it is characterized in that, the dosage form of described compositions is wettable powder, cream, suspending agent, suspension emulsion, microcapsule, seed coat agent, microemulsion, aqueous emulsion, water dispersible granules, foam, unguentum, aerosol, ultra-low volume spray liquid.
10. the compositions described in claim 1 is for preventing and treating the purposes of fungus.
11. compositions according to claim 1 is for preventing and treating the purposes of fungus on Cereal, vegetable, Cotton Gossypii, fruit tree, ornamental plant.
12. the method preventing and kill off plant disease, the bactericidal composition described in claim 1 is applied to plant, plant propagation material, environment.
13. method according to claim 12, it is characterised in that: the propagating materials of plant to be protected is needed by the compositions-treated described in claim 1.
14. the compositions described in claim 1 is for preventing and treating the purposes of fruit vegetables storing period disease.
15. the method protecting seed, it includes making seed prior to seeding and/or contacting with the bactericidal composition described in claim 1 after accelerating germination.
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| CN103271144A (en) * | 2013-06-04 | 2013-09-04 | 浙江大学 | Compound chemical bactericide for control of postharvest diseases of fruits |
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