CN110384100A - A kind of bactericidal composition - Google Patents

Abstract

The present invention relates to a kind of bactericidal compositions, the bactericidal composition for more particularly, to protecting plant or seed to invade from pathogenic epiphyte.The present invention provides a kind of bactericidal composition, and the bactericidal composition contains active constituent biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone, and wherein the weight proportion of biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1:50.The invention further relates to the bactericidal composition be used to control cereal, bean, fruit tree and fruits, greengrocery, root vegetables, forage grass class, turfgrass or turf, spice crop, on flower plant fungi and bacterium purposes.

Description

A kind of bactericidal composition

Technical field

The present invention relates to a kind of bactericidal compositions, and plant or seed is more particularly, to protected to invade from pathogenic epiphyte Bactericidal composition.

Background technique

At present for being agriculturally also easy to produce the disease control of resistance, the pesticide species for using the mechanism of action different carry out mixture It is optimal selection, if proportion is rationally, can produce significant synergistic effect, field control effect is made to be substantially better than each list The effect of agent.Fungicide containing single active ingredient is usually present certain defect, continuous several times in agricultural disease prevention and treatment Using not only making pathogen be also easy to produce drug resistance, and easily cause the pollution to food and environment, by fungicidal activity at point it Between rational mixture can overcome disadvantage mentioned above.The synergistic effect that reasonable compounding generates effective component, can be improved preventive effect, subtracts Few effective ingredient dosage, save the cost delays the generation of the drug resistance of pathogen, and then can reduce or even avoid pesticide to food The pollution of object and environment.

About pesticide activity, especially to crop protection, the key problem for the research carried out in the technical field first is that Improve the performance in terms of performance, especially bioactivity and the performance in terms of keeping this activity within a certain period of time.

Biphenyl pyrazine bacterium amine (Pyraziflumid), CAS:942515-63-1；Molecular structural formula are as follows:

Biphenyl pyrazine bacterium amine is the mould inhibitor class fungicide of succinic acid dehydrogenation of Nihon Nihyaku Co., Ltd's exploitation.Biphenyl pyrazine bacterium Amine is by known in CN101341136A.

Due to now constantly improve to the environmental requirement of fungicide and economic requirement, such as to activity profile, toxicity, selection Property, rate of application, residual and it is advantageous preparation feasibility requirement, additionally since there may be ask in terms of such as drug resistance Topic.Therefore, exploitation is in some aspects lasting task better than the new fungicide of existing fungicide.

Summary of the invention

Object of the present invention is to provide a kind of bactericidal composition in view of the above shortcomings, the bactericidal composition includes to live Property ingredient biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone.

It has now unexpectedly been found that simultaneously, i.e., jointly or separately applying biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone, or successively apply Preferably to prevent and treat plant pathogenic fungi than each compound is administered alone with biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone.

Therefore, the present invention provides a kind of bactericidal composition, the composition is by by biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium Ketone carries out binary built, so that obtained composition has gain effect in control efficiency, and has expanded fungicidal spectrum, has played The multi-purpose effect of one medicine, effectively slows down or germ is avoided to develop drug resistance.Astoundingly, bactericidal composition of the invention Bactericidal activity is more considerably higher than the active adduction of each reactive compound.In other words, there are unpredictable, necessary being Synergistic effect, rather than just active supplement.

Amine benzene pyrrole bacterium ketone (Fenpyrazamine) is that the pyrazolone of SUMITOMO CHEMICAL chemical industry Co., Ltd. exploitation spreads out Biology.Amine benzene pyrrole bacterium ketone in JP2000226374 by disclosing.

When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright It is aobvious.But the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.

A kind of bactericidal composition of the present invention is to adopt the following technical solutions to achieve:

A kind of bactericidal composition, it is characterised in that: containing active constituent biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone, wherein biphenyl pyrrole The weight proportion of piperazine bacterium amine and amine benzene pyrrole bacterium ketone be 50:1-1:50, preferably 20:1-1:40, preferably 10:1-1:40, more preferable 8: 1-1:35；More preferably 4:1-1:35.

The weight proportion of biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone in the present invention for example can also be 50:1,49:1,48: 1,47:1,46:1,45:1,44:1,43:1,42:1,41:1,40:1,39:1,38:1,37:1,36:1,35:1,34:1,33:1, 32:1,31:1,30:1,29:1,28:1,27:1,26:1,25:1,24:1,23:1,22:1,21:1,20:1,19:1,18:1, 17:1,16:1,15:1,14:1,13:1,12:1,11:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1.5: 1,1:1,1:1.5,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1: 16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1: 31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1: 46,1:47,1:48,1:49,1:50。

The bactericidal composition, it is characterised in that: the biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone quality sum account for institute State the 5%-90% of Bactericidal composite amount of substance, more preferable 5%-80%, more preferable 10%-75%, more preferable 10%-70%, more preferable 10%- 65%, more preferable 10%-60%, more preferable 10%-55%, more preferable 10%-50%.

In bactericidal composition of the invention, the content of the biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone accounts for the Bactericidal composite Object by weight can also be, for example: 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%, 35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%, 54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%, 73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89%,90%。

A method of bactericidal composition is acted on pathogenic bacteria and/or its environment, Huo Zhezhi by prevention and treatment plant pathogen In object, plant parts, seed, soil, region, material or space.

A method of biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are administered simultaneously or apply respectively by prevention and treatment plant pathogen With or sequential application.

A kind of bactericidal composition includes biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone, filler and/or surfactant.

A kind of bactericidal composition can be configured to any dosage form agriculturally allowed.The bactericidal composition, dosage form are Aqueous suspension agent, seed treatment suspending agent, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspending agent, granule, missible oil, Microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids, thermal fog, dry suspending agent, oil-suspending agent.

The bactericidal composition for prevent and treat cereal, bean, fruit tree and fruits, greengrocery, root vegetables, Forage grass class, turfgrass or turf, spice crop, flowers etc..

The bactericidal composition is for protecting plant, plant propagation material and the plant organ then grown from fungi With the purposes of bacteria attack.The plant propagation material is seedling, rhizome, garden seedling, cutting or seed.

The bactericidal composition is for handling seed to protect seed from the purposes of fungi and bacteria attack.

The bactericidal composition is used to handle the soil of plant growth to protect the plants from the fungi in soil and thin The purposes of bacterium invasion.

The place prevention and treatment soil that the bactericidal composition is prevented and treated needed for being applied to causes a disease or the use of saprophytic fungi and bacterium On the way.

The bactericidal composition is used to prevent and treat the purposes of fruit vegetables storing period disease.

Bactericidal composition of the invention has very strong activity to various phytopathogens, and can draw to by phytopathogen The prevention and treatment of the plant disease risen play very strong preventive effect.Bactericidal composition of the invention to such as Plasmodiophoromycetes, Oomycete, Chytridiomycetes connect with wide scopes plant pathogenic fungis such as Gammaproteobacteria, Ascomycetes, Basidiomycetes, deuteromycetes with pole Good activity.

Bactericidal composition of the invention also has extraordinary bactericidal performance, and it is thin to can be used for prevention and treatment plant pathogenic Bacterium.Such as pseudomonadaceae, Rhizobiaceae, enterobacteriaceae, rod Cordycepps and Streptomycetaceae.

Bactericidal composition of the invention can be used as foliage fungicide in crop protection, can also be used as fungicide for mixing Plant and be used as soil fungicides.

Bactericidal composition of the invention also can be used for preventing and treating the disease of fruit vegetables storing period.

Bactericidal composition of the invention is suitable for preventing and treating following phytopathogen:

Oomycete, including Phytophthora (Phytophthora), such as phytophthora infestans (Phytophthora infestans), Phytophthora sojae kaufmann&gerdemann (Phytophthora megasperma), foot rot of citrus bacterium (Phytophthora parasitica), The disease of camphor tree phytophthora (Phytophthora cinnamomi) and phytophthora capsici (Phytophthora capsici)；Grass The rotten mould withered disease for belonging to (Pythium) such as level ground grass Pythium aphanidermatu (Pythium aphanidermatum)；And frost Mould section (Peronosporaceae) disease such as Plasmopara viticola (Plasmoparaviticola)；Peronospora (Peronospora) (including Peronosporatabacina (Peronospora tabacina) and Peronospora parasitic bacterium (Peronospora parasitica))；Pseudoperonospora (Pseudoperonospora category) germ (including bacterium of downy mildew of cucumber (Pseudoperono Sporacubensis) and disk obstructs mould germ (Bremialactucae)；Pythium (Pythium) such as scraping and printing unit (Pythium aphanidermatum), black leg of beet bacterium (Pythium ultium)；Plasmopara (Plasmopara) is such as Plasmopara viticola (Plasmopara viticola).

Ascomycetes, including Alternaria (Alternaria) disease such as tomato early blight bacterium (Alternariasolani) and black spot of cabbage bacterium (Alternaria brassicae)；Ball seat Pseudomonas (Guignardia) Disease such as black rot of grape bacterium (Guignardia bidwelli)；The black star of Venturia (Venturia) disease such as apple Germ (Venturia inaequalis)；Septoria (Septoria) disease such as glume blight bacterium (Septorianodorum) and leaf spoting bacteria (Septoriatritici)；Erysiphe (Erysiphe) such as wheat white powder Germ (Erysiphe graminis) and radish Powdery Mildew (Erysiphepolygoni)), grape powdery mildew (Uncinulanecatur), cucumber powdery mildew's pathogen (Sphaerotheca fuligena) and apple mildew bacterium (Podosphaera leucotricha)；Wheat Phyllostachys pubescens (Pseudocercosporella herpotrichoides) Species；Grey mold Pseudomonas (Botrytis) species disease such as Botrytis cinerea germ (Botrytiscinerea), Monilinia fructicola (Moniliniafructicola) disease；Pyrenomycetes category (Sclerotinia) species disease such as Sclerotinia sclerotiorum (Sclerotinia sclerotiorum)；Magnaporthe grisea category (Pyricularia oryzae Cav.) such as Pyricularia oryzae (Magnaporthegrisea)；Compacted shape Pseudomonas (Helminthosporium) species disease such as Exserohilum turcicum (Helminthosporium triticirepentis), reticulate pattern germ (Pyrenophorateres) species；Colletotrichum (Colletotrichum category) such as fine strain of millet anthrax bacteria (Colletotrichum graminicola) and watermelon anthrax bacteria (Colletotrichum orbiculare))；Gaeumannomyce (Gaeumannomyces gramims) such as gaeumannomyces graminis (Gaeumannomyces graminis)；Podosphaera (Podosphaera)；Chain sclerotinia sclerotiorum belong (Monilinia)；Hook Silk shell category (Uncinula)；Mycosphaerella (Mycosphaerella)；Leptosphaeria (Leptosphaeria);Cross hair list capsule Shell category (Blumeria);Sclerotinia category (Sclerotinia);Gaeumannomyce category (Gaeumannomyces);Mycotoruloides (Monilinia).

Basidiomycetes, including the rest fungus disease as caused by Rust (Puccinia) (such as Puccinia recondita (Pucciniarecondita), strip rust bacteria (Pucciniastriiformis), leaf rust (Pucciniahordei), puccinia graminis bacterium (Pucciniagraminis) and handle rest fungus (Pucciniaarachidis), coffee rest fungus (Hemileiavastatrix), camel Spore Rust (Hemileia), phakopsora pachyrhizi sydow (Phakopsorapachyrhizi)；Ustilago (Listilaginalcs).

Deuteromycetes, including Rhizoctonia (Rhizoctonia category) species (such as Rhizoctonia solani Kuhn (Rhizoctoniasolani), redness of the skin or complexion hyphal cluster germ (Rhizoctoniaoryzae), Oryza sheath blight fungus (Rhizoctonia Solani);Fusarium (Fusarium) disease, such as Fusarium graminearum (Fusarium graminearum), beads fusarium Bacterium (Fusarium moniliforme), Fusarium oxysporum (Fusarium oxysporum), fusarium moniliforme (Fusarium ), proliferatum Fusaium solani (Fusarium solani)；Verticillium dahliae (Verticillium dahliae)； Sclerotiumrolfsii (Sclerotium rolfsii)；Moire bacterium (Rynchosporium secalis);Black puckery germ (Cercosporidium personatum), alternaria (Cercospora arachidicola) and brown patch germ (Cercospora beticola);Silver dollar pinta bacterium (Rutstroemia floccosum)；Botrytis (Botrytis)； Pyricularia Sacc. (Pyricularia)；Helminthosporium (Helminthosporium)；Fusarium (Fusarium)；Septoria (Septoria)；Cercospora (Cercospora)；Alternaria (Alternaria) such as Alternaria mali (Alternaria mali), tomato early blight bacterium (Alternaria solani)；Pyricularia Sacc. (Pyricularia);False small tail Spore category (Pseudocercospora) such as sugar beet leaf spot bacteria (Cercospora beticola);Blastocladia bacterium such as peach is black Star germ (Cladosporium carpophilum)；Phoma bacterium such as lucid asparagus Phoma asparagi Sacc (Phomasparagi)；Shell Needle spore category bacterium such as wheat crust blight bacterium (Septoria nodorum);Colletotrichum bacterium such as Colletotrichumlagenarium (Colletotrichum largenirium)；Pyricularia category bacterium such as wilt (Pyricularia oryzae), Botrytis bacterium such as greengrocery ash arrhizus bacteria (Botrytis cinerea)；Apple bivalve (Diplocarpon mali Harada Et Sawamura) and apple Marssonina (Marssonina coronaria) caused by apple marssonina leaf spot.

Bactericidal composition of the invention is particularly suitable for preventing and treating following phytopathogen: Botrytis (Botrytis), pears spore Belong to (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), Alternaria (Alternaria), Pyricularia Sacc. (Pyricularia), false small tail Spore category (Pseudocercospora), Rhizoctonia (Rhizoctonia), camel spore rest fungus category (Hemileia), Puccinia (Puccinia), Phakopsora (Phakopsora), Ustilago (Llstilaginalcs), Venturia (Venturia), Erysiphe (Erysiphe), Podosphaera (Podosphaera), chain sclerotinia sclerotiorum belong (Monilinia), Sclerotinia (Sclerotinia sclerotiorum) Uncinula (Uncinula), mycosphaerella (Mycosphaerella)), Phytophthora (Phytophthora), pythium (Pythium), Plasmopara (Plasmopara), peronospora (Peronospora), Pseudoperonospora (Pseudoperonospora), disk stalk mould (Bremialactucae).

The suitable crop of bactericidal composition of the invention specifically includes that

Cereal (such as rice, barley, wheat, rye, oat, corn, jowar plant), bean (such as soybean, red small The plants such as beans, semen viciae fabae, pea, peanut), fruit tree and fruits (such as apple tree, citrus fruit trees, pear tree, vine, peach, plum Tree or Japanese plum, cherry tree, walnut tree, apricot, Banana tree, strawberry etc. and their fruit), greengrocery (such as wild cabbage, western red The plants such as persimmon, spinach, cauliflower, lettuce, onion, shallot, pumpkin, cucumber, watermelon, muskmelon), root vegetables (such as carrot, potato, The plants such as sweet potato, japanese radish, turnip), insutrial crop or crop (such as cotton, hemp, paper mulberry, oil for industrial processes Dish, beet, hops, sugarcane, sugar beet, Chinese olive tree, rubber tree, coffee tree, tobacco, tea tree etc.), forage grass class (such as orchard Turfgrass, jowar, timothy grass, clover etc.), turfgrass or turf, spice crop etc. (such as lavender, be confused folded perfume, thyme, Parsley, pepper, ginger etc.), flowers (such as chrysanthemum, rose, orchid) etc..

The bactericidal composition of the invention preferably prevention such as following plant disease and control:

The brown patch germ (Cercospora beticola) of " beet ", black root germ (Aphanomyces cochlloides), Pine root fungus (Thanatephorus cucumeris), leaf rot bacterium (Thanatephorus cucumeris), rhizoctonia solani (Pythium ultium);

Brown patch germ (Mycosphaerella arachidis), the alternaria (Mycosphaerella of " peanut " Berkeleyi), pine root fungus (Thanatephorus cucumeris).

Powdery Mildew (Sphaerotheca fuliginea), the Pseudoperonospora cubensis (Pseudoperonospora of " cucumber " Cubensis), didymella bryoniae (Mycosphaerella melonis), dead arm bacterium (Fusarium oxysporum), sclerotium Germ (Sclerotinia sclerotiorum), ash arrhizus bacteria (Botrytis cinerea), anthracnose (Colletotrichum orbiculare), black star germ (Cladosporium cucumerinum), brown patch germ (Corynespora cassicola), seedling blight bacterium (Pythium debaryanam, Rhizoctonia solani Kuhn), bacterial leaf spot (Pseudomonas syringae pv.Lecrymans)；

Ash arrhizus bacteria (Botrytis cinerea), the Fulvia fulva Ciferri (Cladosporium fulvum), late disease bacteria of " tomato " (Phytophthora infestans), pine root fungus (Thanatephorus cucumeris), dry rot germ (Sclerotinia Sclerotiorum)；

The ash arrhizus bacteria (Botrytis cinerea) of " eggplant ", bacterial blight bacterium (Corynespora melongenae), white powder Germ (Erysiphe cichoracearum), suede bacterial plaque germ (Mycovellosiella nattrassii)；

Ash arrhizus bacteria (Botrytis cinerea), the Powdery Mildew (Sohaerotheca humuli), anthrax bacteria of " grass is mould " (Colletotrichum acutatum), epidemic disease bacterium (Phytophthora cactorum)；

The neckrot germ (Botrytis allii) of " onion ", ash arrhizus bacteria (Botrytis cinerea), hickie leaf spoting bacteria (Botrytis squamosa), Pseudoperonospora cubensis (Peronospora destructor)；

The root nodule germ (Plasmodiophora brassicae) of " cabbage ", Bacteria erwinia (Erwinia Carotovora), Pseudoperonospora cubensis (Peronospora parasitica)；

Hyphal cluster germ (Sclerotinia sclerotiorum), the ash arrhizus bacteria (Botrytis cinerea) of " hyacinth bean "；

The Powdery Mildew (Podosphaera leucotricha) of " apple ", black star germ (Venturia inaequalis), Blossom rot bacterium (Monilinia mali), stain germ (Mycosphaerella pomi), rotten pathogenic bacteria (Valsa mali), Spot defoliation bacterium (Alternaria mali), brown spot pathogen (Gymnosporangium yamadae), Target spot pathogen (Botryosphaeria berengeriana), anthrax bacteria (Glomerella cingulata), brown patch germ (Diplocarpon mali), fly pinta bacterium (Zygophiala jamaicensis), sooty mould bacterium (Gloeodes Pomigena), leaf spot fungi；

Powdery Mildew (Phyllactinia kakicola), the anthrax bacteria (Gloeosporium kaki), angle spot of " persimmon " It falls leaves germ (Cercospora kaki)；

The brown rot germ (Monilinia fructicola) of " peach ", black star germ (Cladosporium carpophilum), Phomopsis germ (Phomopsis sp.)；

The brown rot germ (Monilinia fructicola) of " cherry "；

Ash arrhizus bacteria (Botrytis cinerea), the Powdery Mildew (Uncinula necator), late blight bacterium of " grape " (Glomerella cingulata), Colletotrichum acutatum), Pseudoperonospora cubensis (Plasmopara Viticola), hemorrhagic black smallpox germ (Elsinoe ampelina), brown patch germ (Pseudocercospora vitis), black rot Bacterium (Guignardia bidwellii)；

It is the black star germ (Venturia nashicola) of " pears ", brown spot pathogen (Gymnosporangium asiaticum), black Pinta bacterium (Alternaria kikuchiana), Target spot pathogen (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali)；

The zonate spot bacterium (Pestalotia theae) of " tea ", anthrax bacteria (Colletotrichum theae- Sinensis)；

The Streptomyces scabies (Elsinoe fawcetti) of " citrus ", mould germ (Penicillium italicum), green mould Bacterium (Penicillium digitatum), ash arrhizus bacteria (Botrytis cinerea), stain germ (Diaporthe Citri), ulcer bacteria (Xanthomonas campestris pv.Citri)；

The cereal Powdery Mildew (Erysiphe graminis) of " wheat ", cereal reaping hook germ (Fusarium Graminearum), mountain top reaping hook germ (F. culmorum), avenge mould leaf spoting bacteria (Microdochium nivale), wheat Stripe Rust (Puccinia striiformis), stem rust of wheat bacterium (P. graminis), wheat leaf rust germ (P.recondita), rotten reaping hook germ (Micronectriella nivale), wheat loose smut bacterium (Ustilago Tritici), black crooked germ (Tilletia caries), wheat Phyllostachys pubescens (Pseudocercosporella Herpotrichoides), wheat septoria bacterium (Mycosphaerella graminicola), many spores of clever withered shell bacterium (Stagonospora nodorum), wheat De Shi mould bacterium (Pyrenophora tritici-repentis), rhizoctonia solani (Gaeumanomyces graminis), Streptomyces scabies (Fusarium graminearum)；

The cereal Powdery Mildew (Erysiphe graminis) of " barley ", cereal reaping hook germ (Fusarium Graminearum), fusarium avenaceum (F. avenaceum), mountain top reaping hook germ (F. culmorum), avenge mould leaf spoting bacteria (Microdochium nivale), puccinia striiformis (Puccinia striiformis), stem rust of wheat bacterium (P. Graminis), barley leaf rust bacterium (P.hordei), naked smut bacterium (Ustilago nuda), barley leaf blotch bacterium (Rhynchosporium secalis), net blotch of barley bacterium (Pyrenophora teres), barley A. mali (Cochliobolus sativus), stripe disease of barley bacterium (Pyrenophora graminea), sheath blight fungus (Rhizoctonia solani);

The rice blast (Pyricularia oryzae) of " rice ", banded sclerotial blight (Rhizoctonia solani), bakanae disease (Gibberella fujikuroi), flax leaf spot (Cochliobolus niyabeanus), seedling blight bacterium (Pythium Graminicola), leaf spot bacteria (Xanthomonas oryzae), bacillary seedling blight bacterium (Burkholderia Plantarii), the withered bacillary germ (Burkholderia glumae) of brown streak bacterium (Acidovorax avenae), paddy, sheath Blight bacterium (Pyricularia oryzae), striped germ (Cochiobolus Miyabeanus)；

The sclerotinite (Sclerotinia sclerotiorum) of " rape straw ", sheath blight fungus (Rhizoctonia solani)；

The sclerotiniose (Sclerotinia sclerotiorum) of " tobacco ", powdery mildew (Erysiphe cichoracearum), Tobacco brown spot pathogen (Alternaria longipes), two spore powdery mildews (Erysiphe cichoracearum), tobacco anthrax Germ (Colletotrichum tabacum), Peronosporatabacina (Peronospora tabacina), the black chamber germ of tobacco (Phytophthora nicotianae)；

The gray mold (Botrytis cinerea) of " tulip "；

Snow big grain sclerotinia rot (Sclerotinia borealis), the samping off (Pythium of " Western Korea lawn grass " Aphanidermatum)；

The powdery mildew (Erysiphe graminis) of " orchard grass "；

The Cercospora kikuchii (Cercospora kikuchii) of " soybean ", soybean Elsinochrome (Elsinoe glycines), Seat shell soya bean varieties (Diaporthe phaseolorum var.sojae), Soybean Brown Spot line septoria musiva (Septoria Glycines), Germ To Soybean Frogeye Leaf Spot (Cercospora sojina), Phakopsora pachyrhizi (Phakopsora pachyrhizi), Pathogen of soybean root rot (Phytophthora sojae), sheath blight fungus (Rhizoctonia solani), stick spore leaf spot (Corynespora casiicola), sclerotinite (Sclerotinia sclerotiorum)；

The smut (Ustilago maydis) of " corn ", different cochliobolus (Cochliobolus heterostrophus), Jowar cercospora glue bacterium (Gloeocercospora sorghi), Puccinia polysora (Puccinia polysora), grey leaf spot disease of maize Bacterium (Cercospora zeae-maydis), sheath blight fungus (Rhizoctonia solani)；

The phytophthora infestans (Phytophthora infestans) of " potato ", potato phytophthora pink disease bacterium (Phytophthora erythroseptica), powdery scab of potato bacterium (Spongospora subterranean)；

Humulus Pseudoperonospora cubensis Pseudoperonospora bacterium (Pseudoperonospora humuli);

Alternaric bacteria (Alternaria japonica), the hickie germ (Cercosporella of brassicaceous vegetable Brassicae), plasmodiophora brassicae (Plasmodiophora brassicae), Peronospora parasitic bacterium (Peronospora parasitica)；

Onion mildew bacterium (Peronospora destructor);

Downy mildew of spinach bacterium (Peronospora spinaciae)；

Plurality of plant diseases: level ground grass Pythium aphanidermatu (Pythium aphanidermatum), Pythium debarianum, P.graminicola Subram (Pythium graminicola), abnormal female pythium spp (Pythium irregulare), Pythium ultimum bacterium (Pythium ultimum), the pathogen of Botrytis cinerea (Botrytis cinerea), sclerotinite (Sclerotinia Sclerotiorum), chain sclerotinia sclerotiorum (Monilinia Spp.).

Bactericidal composition of the invention is particularly suitable for controlling following plant disease: brown spot, spot defoliation on apple, Ring spot, anthracnose, shot hole；Rice sheath blight disease, rice blast, false smut；Wheat leaf rust, powdery mildew, leaf blight, grain husk are withered Disease, head blight, full rot；Grape grey mould, downy mildew, white rot, powdery mildew, Spike-stalk Brown Spot；Target, late blight Disease, tar spot, target spot；Barley powdery mildew, net blotch；Wax gourd downy mildew, anthracnose；Early blight of tomato, late blight, leaf mold Disease, gray mold；Mandarin tree shot hole, anthracnose；Scab of cucumber, blight dis-ease, powdery mildew, gray leaf spot, target disease, downy mildew Disease；Pepper anthracnose, epidemic disease；Sponge gourd downy mildew；Sigatoka, brown spot, scab；Powdery mildew of strawberry；Watermelon powdery mildew, Blight dis-ease；The leaf blight of corn, helminthosporium maydis；Watermelon anthrax, blight dis-ease；Tea tree anthracnose；Brown patch disease, giant spot disease, snow are rotten Disease；Sclerotinia sclerotiorum；Cabbage Leaf Spot；Peanut morning pinta；Soybean rust；Jujube tree rust；Coffee rust.

Bactericidal composition of the invention is especially effective to the disease of following crop: brown spot, spot defoliation on apple, Ring spot, anthracnose, shot hole；Powdery mildew, the gray mold of more crops；Rice sheath blight disease, rice blast；Cabbage Leaf Spot；Lawn Giant spot disease；Wheat leaf rust；Early blight of tomato；Sclerotinia sclerotiorum.

Bactericidal composition of the invention is suitable for the prevention and treatment disease as caused by bacterium as follows: for example by pseudomonas (Pseudomonas), disease caused by xanthomonas (Xanthomonas), Erwinia (Erwinia) etc..

Example as the disease as caused by bacterium has:

Disease caused by pseudomonas (Pseudomonas), if the bacillary spot blight of cucumber is (by pseudomonas syringae Pseudomonas syringae pv. Iachrymans) cause)；The bacterial wilt of tomato is (by Ralstonia solanacearum (Ralstonia solanacearum) causes)；The bacillary cereal blight (grain rot) of rice plants is (by glume blight bacterium (Burkholderia glumae) causes)；

The disease as caused by xanthomonas (Xanthomonas), if the black rot of wild cabbage is (by xanthomonas campestris (Xanthomonas campestris) causes)；The Bacterial Leaf Blight of rice plants is (by bacterial blight of rice Flavobacterium (Xanthomonas oryzae) causes) and citrus fruit trees canker (cankar) (by citrus ulcer bacterium (Xanthomonas citri) causes)；The disease as caused by Erwinia (Erwinia), such as the bacterial soft rot of wild cabbage (being caused by carrot soft rot Erwinia (Erwinia carotovora)) etc..

The crop of bactericidal composition processing of the invention is such as but not limited to cereal, bean, fruit tree and water Fruit, greengrocery, root vegetables, insutrial crop or crop for industrial processes, forage grass class, lawn class or turf, fragrance Crop, flowers etc..

Bactericidal composition of the invention can also be used to prevent and treat fruit vegetables storing period disease.Such as the fruit as caused by following pathogen It is real to rot:

Aspergillus bacterium, such as aspergillus flavus；

Botrytis (Botrytis) strain, such as Botrytis cinerea (Botrytis cinerea);

Penicillium (Penicillium) strain, for example, penicillium expansum (Penicillium expansum) and P.purpurogenum;

Sclerotinia (Sclerotinia) strain, such as sclerotinite (Sclerotinia sclerotiorum);

Verticillium (Verticilium) strain, such as black and white wheel branch spore (Verticilium alboatrum).

Bactericidal composition of the invention be also particularly well suited for prevention and treatment fruit vegetables storing period disease blossom-end rot, green mould, penicilliosis, Anthracnose.

Fungicidai mixtures of the invention are further adapted for prevention and treatment kind biography and soil-borne disease.Such as kind of the biography as caused by following pathogen The rotten and wilting disease and seedling disease passed with soil:

Alternaria belongs to kind, such as rape life rod method (Alternaria brassicicola);

Aphanomyces (Aphanomyce) category kind, such as Kidney bean silk capsule are mould (Aphanomyces euteiches);

Ascochyta (Ascochyta) belongs to kind, such as Ascochyta lentis;

Aspergillus, such as aspergillus flavus;

Cladosporium belongs to kind, such as draft branch spore (Cladosporium herbarum);

Cochliobolus belongs to kind, such as standing grain cochliobolus;

Colletotrichum belongs to kind, such as hair core anthrax-bacilus (Colletotrichum coccodes);

Fusarium belongs to kind, such as yellow fusarium;

Gibberella category kind, such as beautiful another name for Sichuan Province multitude are red mould;

Shell ball spore category (Macrophomina) belongs to kind, such as Kidney bean shell ball spore (Macrophominaphaseolina);

Small setting-out shell category strain, such as the rotten small setting-out shell of snow;

Penicillium belongs to kind, such as penicillium expansum;

Phaeosphaeria strain, such as Phaeosphaeria nodorum;

Phoma (Phoma) belongs to kind, such as black embryo Phoma sp (Phoma lingam);

Phomopsis (Phomopsis), such as soybean Phomopsis (Phomopsis sojae);

Phytophthora belongs to kind, such as Phytophthora cactorum (Phytophthora cactorum);

Pyrenophora belongs to kind, such as wheat nuclear cavity bacteria (Pyrenophora graminea);

Pyricularia Sacc. (Pyricularia) belongs to kind, such as Magnaporthe grisea (Pyricularia oryzae);

Pythium kind, such as Pythium ultimum;

Rhizoctonia belongs to kind, such as Rhizoctonia solani Kuhn;

Rhizopus (Rhizopus) belongs to kind, such as Rhizopus oryzae (Rhizopus oryzae);

Bulbil category (Sclerotium) belongs to kind, such as Sclerotium rolfsii (Sclerotium rolfsii);

Core coral Pseudomonas (Typhula) belongs to kind, such as meat spore core coral bacterium (Typhula incarnata);

Verticillium (Verticillium) belongs to kind, such as verticillium dahliae (Verticillium dahliae).

Bactericidal composition of the invention can spread by seed treatment, cauline leaf, soil application or used for ponds etc. Prevention and treatment for the various diseases generated when cultivation includes the agriculture garden crop of flowers, lawn, herbage.

The present invention provide it is a kind of prevent and treat plant pathogen method, by the bactericidal composition act on pathogenic bacteria and/ Or in its environment or plant, plant parts, plant propagation material, soil, region, material or space.

The present invention also provides a kind of control plant, plant part, plant propagation material and the subsequent plant organs that grow The method of plant pathogenic fungi, including by bactericidal composition of the invention with agronomy is effective and the amount of application of basic plant-less toxicity It applied, be impregnated with seed treatment, foliage applying, stem, instiled, is poured, sprayed, is sprayed, dusting, the methods of distribution or smoke application It is growing or is needed in the soil or cultivation medium wherein grown to plant, plant part, plant propagation material or plant.

Bactericidal composition of the invention can be used for the plant device protecting plant part, plant propagation material and then growing Official.

Bactericidal composition of the invention can handle all plants and plant part." plant " refers to all plants and plant Population, such as ideal and undesirable wild plant, cultivated plant and plant variety are (regardless of whether by plant variety or plant Cultivate the protection of human rights benefit).Cultivated plant and plant variety can be the plant obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique methods, such as use dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Part and organ above and below the ground, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.It crop and nourishes and generates and case of propagation material, such as transplants, bulb, rhizome, running roots Plant part is also belonged to seed.

Term " plant propagation material " is interpreted as referring to all plant parts for having fertility, such as seed, can use In breeding the latter and vegetable matter such as cuttage item or stem tuber (such as potato).Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.To The germination plant and effective plant inhibited after germinateing in soil or after emergence.Young plant can be before transplantation by being impregnated into Row whole or Local treatment are protected.

Currently preferred propagation material is seed.The method of seed treatment, such as can there are, diluent liquid or solid Seed is directly immersed in liquid condition solution the method for making medicament be impregnated with seed without dilution, by solid by the medicament of shape Medicament or liquid preparation are mixed with seed, and being coated processing makes the method for the surface of the seed attachment medicament, in plantation The methods of sprinkling near seed simultaneously.

Plant part and the plant organ then grown are any of the plant generated by plant propagation material such as seed Part.Plant part, plant organ and plant also can benefit from by the way that bactericidal composition is applied to plant propagation material institute The pathogenic damage of acquisition is protected.The plant organ grown behind certain plants part and certain places can also regard plant propagation as Material, its own can apply (or processing) with bactericidal composition；To by processed plant part and processed plant Plant, other plant parts and the other plant organs that organ generates also can benefit from by applying bactericidal composition With.

Seed and seedling are subject to the harm of a variety of pathogens and infect, and are the links of most fragile in process of crop growth； Are there is sick seedling, seedling death phenomenon by pathogen infection in seed, seedling, can be reduced effective strain number of unit area crop, be caused crop It drops in production over a large area and quality decline.

Bactericidal composition of the invention be suitable for protect plant propagation material such as seed, fruit, stem tuber or kernel or Person's plant cutting is not infected by phytopathogen.Can the propagation material, example be handled with the bactericidal composition before administration As dressed seed prior to seeding.Can also by liquid composition impregnate kernel or with solid composite be coated with kernel, by activity at It gives and is added in kernel (coating).It, can also should when propagation material is applied to that for example seed is broadcast in ditch in seeding process Composition is applied to application site.These plant propagation materials handled to the processing method of plant propagation material and so are all Further subject matter of the present invention.

It, can also be by being impregnated in succession with the liquid preparation of each active constituent in order to handle plant propagation material especially seed Stem tuber or grain, or by being coated with the wet or dry preparation combined.

Seed treatment application of the invention by prior to seeding and/or after vernalization by seed is sprayed or and dusting into Row.

Bactericidal composition of the invention is applied to seed with itself or suitable dosage form.It is preferred that seed is in stable state Under it is processed so that processing do not cause any damage.It usually can be any between harvesting and sowing to the processing of seed Time carries out.

It is preferred that being handled before seed sowing, so as to be pre-processed with bactericidal composition to the seed broadcasted sowing. Specifically, preferably by seed pelleting or by seed-pelleting in bactericidal composition processing of the invention.Processing as a result, Active constituent in the bactericidal composition is attached on seed, so as to the prevention and control for disease.

By using seed treatment compositions processing seed to protect seed and rudiment plant to invade from plant-pathogenic bacterium Dye is known for a long time and is to continuously improve.However, processing seed also bring it is a series of be not can find with Satisfactory manner solves the problems, such as.Therefore, it is necessary to develop the method for protection seed and rudiment plant, it can exempt and plant Additionally apply crop protection products again afterwards or after plant germination.In addition, it is also necessary to optimize the amount of reactive compound used, so as to Best protection is provided to seed and rudiment plant, to prevent the invasion of plant pathogen, and reactive compound used will not damage Evil plant itself.

But, it is undesirable to effect be the germination rate that processed seed is reduced using seed treatment compositions.Generally Ground can be reduced with the germination rate of the processed seed of seed treatment compositions with the time after application seed treatment, in It is to limit the shelf life of processed seed.Current way is the selection of time of seed treatment in such as field seed Processing will sow preceding processing, to avoid the serious damage to crop.On the other hand, by seed be exposed to it is for example cold, It may aggravate the toxicity damage for the plant that agricultural chemicals generate seed under the environmental pressures such as dry.

It has to be noticed that being applied to the amount of the bactericidal composition of the present invention of seed and/or other usually during seed treatment The amount of additive is selected as the germination that will not influence seed or will not damage plant.

Bactericidal composition of the invention is for processing plant propagation material, especially rice, rape, cotton, wheat, big Wheat, soybean, corn, peanut seed be particularly advantageous.

The present invention also provides a kind of methods for protecting seed comprising after making seed prior to seeding and/or vernalization and originally The bactericidal composition of invention contacts.

Therefore, more particularly, the present invention a kind of using bactericidal composition processing seed of the invention to protect seed and The method that germinating plants are invaded and harassed from plant pathogen.

Moreover, it relates to which bactericidal composition of the invention is for handling seed to protect seed and germinating plants to exempt from The purposes infected by phytopathogenic fungi.

Moreover, it relates to be protected from the kind of plant pathogen invasion through bactericidal composition processing of the invention Son.

Bactericidal composition of the invention can also be used to prevent or control a variety of pathogenic or saprophytic in soil or cultivation medium Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), Botrytis cinerea (Botrytis cinerea), Cercospora (Cercospora spp.), ergot (Claviceps purpurea), standing grain cochliobolus (Cochliobolus sativus), Colletotrichum (colletotrichum Spp.), Epicoccum (Epicoccum spp.), F.graminearum schw (Fusarium graminearum), rice dislike seedling chain spore (Fusarium moniliforme), fusarium oxysporum (Fusarium oxysporum, fusarium moniliforme (Fusarium Proliferatum), fusariun solani (Fusarium solani) is tieed up glue chain spore (Fusarium subglitinans), and length is compacted Spore category (Helminthosporium spp) avenges rotten microtorr bacterium (Microdochium nivale), Penicillium (Pencillium Spp), Phoma (Phoma spp.), wheat nuclear cavity bacteria (Pyrenophora graminea), rice blast Pyricularia Sacc. (Pyricularia oryzae), Rhizoctonia solani Kuhn (Rhizoctonia solani), Rhizoctonia cerealis (Rhizoctonia Cerealis), Sclerotinia (Sclerotinia spp.), Septoria (Septoria spp.), silk axis smut (Sphacelotheca reilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula Incarnate), hidden smut (Urocystis occulta), Ustilago (Ustilago spp.) or Verticillium (Verticillium spp.)。

Soil germ has alternaric bacteria, rhizoctonia solani, sickle-like bacteria, phytophthora, bacterium of dampinging off, root rot, pythium spp, grey mold Bacterium, soft-rot fungi etc..Under normal circumstances, soil germ can generate a large amount of thallus, if condition it is advantageous to pathogen growth development and Host be again it is susceptible, germ mass propagation and can infect host, in the presence of susceptible host, these germs Into the lasting pathogenic phase, with the continuous cropping of crop, mass propagation is spread, but later nutrient be depleted or edaphic condition such as When temperature, humidity etc. are unfavorable to germ, germ can enter dormant period again.In the absence of susceptible host, soil-borne disease bacterium is in soil It can also be survived in earth, in addition to soil germ has extensive host range, moreover it is possible to be fallen in the root surface of non-host or residual branch It survives on leaf, is undivided with it with saprophytic competitiveness.But different germs be it is discrepant, as sickle-like bacteria, rod method Bacterium can almost be survived in the soil with indefinite duration.

Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, such as: soil, water etc., Specific raw material can be used for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Sawdust, bark etc..

The method that medicament is applied into soil, including " bactericidal composition " is sprayed on soil method, by " Bactericidal composite The method and " bactericidal composition " is irrigated into the method into soil that object " is mixed with soil.

Therefore, the present invention also provides the bactericidal compositions to be used to handle the soil of plant growth to protect the plants from The purposes of pathogenicity harmful fungoid or bacterial invasion in soil.

A method of prevention and treatment plant pathogen, this method include by bactericidal composition of the invention with agronomy effectively and base The amount of application of this plant-less toxicity applied with seed treatment, foliage applying, stem, is impregnated with, instiled, is poured, sprayed, is sprayed, dusting, The methods of distribution or smoke are applied to plant, plant propagation material and the plant organ then grown, cultivation medium, material or sky Between in.

Therefore, the present invention provides a kind of method for preventing and treating plant pathogen, and this method includes by sterilization of the present invention Composition is applied to plant leaf surface.

The present invention provides a kind of method for preventing and treating plant pathogen, and this method includes by bactericidal composition of the present invention The plant organ for being applied to plant propagation material and then growing.

The present invention provides a kind of method for preventing and treating plant pathogen, and this method includes by bactericidal composition of the present invention It is applied to cultivation medium.

The present invention provides a kind of method for preventing and treating plant pathogen, and this method is included in plant and before disease infestation or is invaded After dye by the bactericidal composition act on plant, plant propagation material and then grow plant organ, cultivation medium, In material or space.

A method of prevention and treatment plant pathogen, this method includes that biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are administered simultaneously Or sequential application.

Another object of the present invention provides a kind of control plant, plant part, plant propagation material and then grows Plant organ, plant culture medium plant pathogenic fungi method, this method include by bactericidal composition of the invention with Agronomy is effective and the amount of application of basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instiled, be poured, sprayed Penetrate, be sprayed, dusting, the methods of distribution or smoke be administered to plant, plant part, plant propagation material or plant be growing or It needs in the cultivation medium wherein grown, preferably soil.

Bactericidal composition of the invention can be applied by different processing methods, these methods for example:

Liquid comprising the bactericidal composition is sprayed onto the aerial part of the plant；

Dusting mixes particle or powder in the soil, sprays in the surrounding plants, and the tree injection or smearing the case where Under；

Cladding or film coated are carried out to the seed of plant.

When for postharvest fruit and vegetable anti-corrosive fresh-keeping, 200-2000 times of liquid is usually diluted with water, is leached after soaking fruit.

The present invention provides a kind of method for preventing and treating plant pathogen, can be treatment, prevention or method of eradication.

The invasion of bactericidal composition of the invention for disease described in protection plant resistant in certain time after treatment. The time of protective effect obtained usually is from plant and has handled continuity 1 to 10 day, preferably 1 to 7 day by composition.Or For up to 200 days after handling seed.

Bactericidal composition of the invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid The dosage forms such as agent, granule, seed coat agent use, amount of application, according to the mixing ratio, meteorological condition, medicament of effective component Form, Dressing date, method of administration, application place, prevent and kill off object noxious livings, objective crop etc. it is different and variant.

A method of biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are administered simultaneously or apply respectively by prevention and treatment plant pathogen With or sequential application.

Processing method of the invention can also be used in handle propagation material such as stem tuber or rhizome, and can be used for handling seed, Seedling or transplanting (pricking out) seedling and plant or transplanting plant.The processing method can also be used for processing root.The present invention Processing method can also be used for the aerial part of processing plant such as dry, stem or stalk, leaf, flower and fruit in relation to plant.

Generally for leaf portion processing: 0.1-10000g/ha, preferably 10-1000 g/ha, more preferable 50-500 g/ha；It is right For dipping or application of instiling, the dosage can also even be reduced, especially when application inert base such as asbestos or pearl When rock；

For seed treatment: 2-5000g/100kg seed, preferably 3-1000g/100kg seed；

For soil or processing used for ponds: 0.1-10000g/ha, preferably 1-1000g/ha；

It is fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, is leached after soaking fruit.

Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to practical feelings when reality is applied Condition and needs adjust rate of application especially in accordance with the seriousness of the property and germ of plant to be processed or crop.

Bactericidal composition of the invention can also be used to that Stored Product be protected to infect from germ.The suitable example of the product Including stem, leaf, seed, fruit or cereal, can be protected under fresh harvesting state or when processing, as predrying, humidification, It crushes, grind, squeeze out or toasts.

Bactericidal composition of the invention shows good Fungicidally active, and the Fungicidally active of single active constituent compared with Weak, the activity of bactericidal composition of the invention is more than the active simple adduction of each single compound

Biphenyl pyrazine bacterium amine of the invention combines/combined administration with amine benzene pyrrole bacterium ketone.Including separating, sequentially or simultaneously applying biphenyl Pyrazine bacterium amine and amine benzene pyrrole bacterium ketone.Preferably, the biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone group are combined into comprising biphenyl pyrazine bacterium The form of the composition of amine and amine benzene pyrrole bacterium ketone.

Bactericidal composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition Ingredient can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with Based on dosage form provided by the invention.

As a further improvement of the present invention, bactericidal composition of the invention can be configured to any agent agriculturally allowed Type.

As a further improvement of the present invention, the dosage form of bactericidal composition of the invention be aerosol, it is capsule suspension, cold It is atomized concentrating agents, heat atomization concentrating agents, CG/Encapsulated granule, granula subtilis, instant solution, sprayable pulvis, emulsifiable concentrating agents, water Oil-in emulsion, water-in-oil emulsion, bulky grain agent, fine granule, the dispersible powder of oil, the miscible flowable concentrating agents of oil, Oily miscible liquids, foaming agent, paste, suspension concentrating agents, solvable concentrating agents, suspending agent, seed coat agent, wettable powder, moisture Shot agent, soluble powder, microcapsule suspending agent, coated granule, squeeze out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, The cold atomization preparation of ultra low volume liquids, suspoemulsion, ultra-low volume, super-low capacity calorimetric atomization preparation, double pack (twin pack), Seed treatment dry powder doses, seed treatment emulsion, seed treatment suspending agent, seed treatment liquor, seed treatment dispersible pulvis, kind Subprocessing microcapsule suspending agent, suspoemulsion, milk particle agent, ultra-low volume suspending agent, ultra low volume liquids, can divide seed treatment gel Dissipate property concentrating agents.

It include biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone, filler and/or surface in bactericidal composition of the present invention Activating agent.

In the bactericidal composition, the biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone quality sum account for the Bactericidal composite The 5%-90% of amount of substance, more preferable 5%-80%, more preferable 10%-75%, more preferable 10%-70%, more preferable 10%-65%, more preferably 10%-60%, more preferable 10%-55%, more preferable 10%-50%.

According to the present invention, term " filler ", which refers to, can be combined or be combined with reactive compound to make it easier for being administered to The natural or synthetic organic or inorganic compound of object (such as plant, crop or careless class).Therefore, the filler is preferably It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.

The nonactive medium that can be used in the present invention can be used as solid matchmaker either solid is also possible to liquid What dielectric material used has for example: phyteral powdery type (such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut Shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem particle, the residue etc. after extracting plant essence), paper It opens, sawdust, crushes synthesized polymer body, the clay class (such as kaolin, bentonite, acid china clay etc.), talcum powder of synthetic resin etc. Class.Silica (such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates), active carbon, natural mineral matter class (float stone, Lv Po Thread stone and zeolite etc.), fire diatomite, sand, (such as polyethylene, polypropylene, the polyvinylidene chloride etc.), chlorination such as plastics medium Chemical fertilizer, the soil of the inorganic mineral powder of potassium, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, ammonium chloride etc. Fertilizer, these substances can be used alone or two or more is mixed.

It can be used as can selecting in llowing group of materials for liquid media materials'use, such as water, alcohol type (such as first Alcohol, ethyl alcohol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng), ethers (such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.), aliphatic hydrocarbon class (such as kerosene, Mineral oil etc.), aromatic hydrocarbons class (such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro rouge Fat hydrocarbon, chlorobenzene, etc.), halogenated hydrocarbon class, amides, sulfone class, dimethyl sulfoxide, mineral and vegetable oil, animal oil etc..

To make the emulsification of effective component compound, dispersion and/or wetting, surfactant can be used for example can be with Enumerate fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the aliphatic ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as object, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine combination.

To make effective component compound dispersion stability, attachment and/or combination, such as xanthan gum, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid (such as cephalin and lecithin) with And the adjuvants such as synthetic phospholipid, bentonite, sodium lignin sulfonate.

The wherein optional spent glycol of antifreezing agent, propylene glycol, glycerine, sorbierite.Deflocculant as suspension product The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.

Organic silicon defoamer can be used in defoaming agent.

The colorant that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff: alizarin dyes, azo dyes and metallized phthalocyanine dye；And microelement, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.

Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.

The reactive compound can be mixed preparation with conventional additives by known way by the preparation of the invention. The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersing agent, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, defoaming agent, preservative, increasing Thick dose, water and other processing aids.

Bactericidal composition of the invention not only includes can be by suitable equipment as spraying or dusting equipment is suitable for immediately Object to be processed, but also including needing the concentration commercial composition being diluted before being applied to object.

The bacterium amine of pyrazine containing biphenyl and amine benzene pyrrole bacterium ketone of the invention can also be applied with other active ingredient combinations, such as with In expansion activity profile or prevent from forming resistance.Other active constituents such as fungicide, attractant, kills elder brother at bactericide Worm agent, acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..

Reactive compound biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone can be administered simultaneously, or apply respectively or sequential application, point Sequence when application is opened on the result of prevention and treatment usually without influence.

Composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition at Dividing can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.

For bactericidal composition of the invention in the case where reducing reactive compound application total amount, having to harmful fungoid improves activity (synergy).And bactericidal composition of the invention shows that the bacterium of tolerance also has excellent kill to existing fungicide Bacterium effect.

A kind of bactericidal composition of the present invention, the composition are answered by the way that biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are carried out binary Match, so that obtained composition has gain effect in control efficiency, and expanded fungicidal spectrum, plays what a medicine was mostly used Effect, effectively slows down or germ is avoided to develop drug resistance.The bactericidal activity of bactericidal composition of the invention is than each active ingredient The active adduction of object is considerably higher, and there are unpredictable, necessary being synergistic effects, rather than just active increasing It mends.

When reactive compound is present in bactericidal composition of the invention with specific weight ratio, synergistic effect is especially bright It is aobvious.But the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.

Specific embodiment

First passing through specific example of formulations below, the invention will be further elaborated.

Example of formulations

1: 60% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone wettable powder of embodiment

Biphenyl pyrazine bacterium amine 10%, amine benzene pyrrole bacterium ketone 50%, lignosulfonates 5%, spaonin powder 4%, attapulgite adds to 100%, Aforementioned formula material is mixed, in a stirring kettle uniform stirring, is remixed after airslide disintegrating mill uniformly, obtain 60% biphenyl pyrrole Piperazine bacterium amine amine benzene pyrrole bacterium ketone wettable powder.

Embodiment 2:55% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone wettable powder

Biphenyl pyrazine bacterium amine 5%, amine benzene pyrrole bacterium ketone 50%, naphthalene sulfonic acid-formaldehyde condensation product 8%, lauryl sodium sulfate 5%, kaolin 100% is added to, aforementioned formula material is mixed, in a stirring kettle uniform stirring, is being uniformly mixed after airslide disintegrating mill, is being made 55% Biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone wettable powder.

Embodiment 3:45% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone wettable powder

Biphenyl pyrazine bacterium amine 5%, amine benzene pyrrole bacterium ketone 40%, MODIFIED LIGNOSULPHONATE 5%, lauryl sodium sulfate 5%, diatomite 100% is added to, aforementioned formula material is mixed, in a stirring kettle uniform stirring, is being uniformly mixed after airslide disintegrating mill, is being made 45% Biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone wettable powder.

Embodiment 4:27% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone water dispersible granules

Biphenyl pyrazine bacterium amine 2%, amine benzene pyrrole bacterium ketone 25%, alkyl benzene sulfonate calcium salt 10%, lauryl sodium sulfate 5%, ammonium sulfate 2%, bentonite adds to 100%, and aforementioned formula material is uniformly mixed, and with micro jet, is mediated, fluidized bed is then added 27% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium is mixed to prepare through sampling analysis after being granulated, dried in granulating and drying machine, being sieved Ketone water dispersible granules.

Embodiment 5:35% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone water dispersible granules

Biphenyl pyrazine bacterium amine 5%, amine benzene pyrrole bacterium ketone 30%, aliphatic acid polyethenoxy ether 5%, lauryl sodium sulfate 4%, kaolin 100% is added to, aforementioned formula material is uniformly mixed, with micro jet, is mediated, fluidized bed prilling drying is then added 35% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone water dispersion is mixed to prepare through sampling analysis after being granulated, dried in machine, being sieved Granula.

Embodiment 6:60% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone water dispersible granules

Biphenyl pyrazine bacterium amine 5%, amine benzene pyrrole bacterium ketone 55%, octylphenol polyethylene oxyethylene group ether sulfate 5%, neopelex 5%, sodium carbonate 2%, white carbon black adds to 100%, and aforementioned formula material is uniformly mixed, and with micro jet, is mediated, then It is added in fluidized-bed granulation dryer after being granulated, drying, sieve and obtains 60% biphenyl pyrazine bacterium amine through sampling analysis Amine benzene pyrrole bacterium ketone water dispersible granules.

Embodiment 7:30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspending agent

Biphenyl pyrazine bacterium amine 5%, amine benzene pyrrole bacterium ketone 25%, polycarboxylate 6%, silicone oil 0.3%, xanthan gum 0.9%, diethylene glycol (DEG) 2.5%, Deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to crossing effective component, by high speed shear It is uniformly mixed, effective component is added, ball milling 2 ~ 3 hours, make partial size all at 5 μm hereinafter, obtaining 30% in the ball mill Benzene pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspending agent.

Embodiment 8:32% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspending agent

Biphenyl pyrazine bacterium amine 2%, amine benzene pyrrole bacterium ketone 30%, naphthalene sulfonic acid-formaldehyde condensation product 6%, hydroxyethyl cellulose 0.7%, silicone Compound 0.1%, polyethylene glycol 2%, deionized water adds to 100%, by its after thickener and antifreezing agent mixing in addition to effective component Remaining component is uniformly mixed by high speed shear, effective component is added, ball milling 2 ~ 3 hours, make partial size all 5 in the ball mill μm hereinafter, obtaining 32% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspending agent.

Embodiment 9:42% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspending agent

Biphenyl pyrazine bacterium amine 2%, amine benzene pyrrole bacterium ketone 40%, lignosulfonates 5%, aluminum magnesium silicate 1%, silicone 0.2%, glycerine 2.8%, deionized water adds to 100%, remaining component after thickener and antifreezing agent are mixed in addition to effective component, by cutting at a high speed Cut it is uniformly mixed, be added effective component, ball milling 2 ~ 3 hours, make partial size all at 5 μm hereinafter, obtaining 42% in the ball mill Biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspending agent.

Embodiment 10:30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspoemulsion

Biphenyl pyrazine bacterium amine 25%, amine benzene pyrrole bacterium ketone 5%, N-Methyl pyrrolidone 20%, alkyl benzene sulfonate calcium salt 5%, Methyl cellulose Element 1%, propylene glycol 3%, Tween 80 7%, water adds to 100%；Finely ground biphenyl is added in the continuous phase of amine-containing benzene pyrrole bacterium ketone The suspended phase of pyrazine bacterium amine is mixed to prepare 30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspoemulsion.

Embodiment 11:5% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone microemulsion

Biphenyl pyrazine bacterium amine 0.5%, amine benzene pyrrole bacterium ketone 4.5%, cyclohexanone 5%, ethoxylated castor oil 4%, dodecyl benzene sulfonic acid Calcium 5%, N-Methyl pyrrolidone 20%, polyethylene glycol 2%, tributyl phosphate 1.4%, deionized water add to 100%, and 5% biphenyl is made Pyrazine bacterium amine amine benzene pyrrole bacterium ketone microemulsion.

Embodiment 12:65% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone water dispersible granules

Biphenyl pyrazine bacterium amine 5%, amine benzene pyrrole bacterium ketone 60%, octylphenol polyethylene oxyethylene group ether sulfate 5%, neopelex 3%, sodium carbonate 1%, white carbon black adds to 100%, and aforementioned formula material is uniformly mixed, and with micro jet, is mediated, then It is added in fluidized-bed granulation dryer after being granulated, drying, sieve and obtains 65% biphenyl pyrazine bacterium amine through sampling analysis Amine benzene pyrrole bacterium ketone water dispersible granules.

Embodiment 13:5% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone aqueous emulsion

Biphenyl pyrazine bacterium amine 0.5%, amine benzene pyrrole bacterium ketone 4.5%, ethoxylated castor oil 6%, propyl alcohol 4%, dodecyl sodium sulfonate calcium 3%, xanthan gum 0.7%, propylene glycol 2.4%, n,N-Dimethylformamide 10%, deionized water add to 100%, and 5% biphenyl pyrazine is made Bacterium amine amine benzene pyrrole bacterium ketone aqueous emulsion.

Embodiment 14:10.5% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone missible oil

Biphenyl pyrazine bacterium amine 0.5%, amine benzene pyrrole bacterium ketone 10%, ethoxylated castor oil 3%, calcium dodecyl benzene sulfonate 2%, N- Methyl pyrrolidone adds to 100%, stirs to transparent uniform phase is obtained, obtains 10.5% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone Missible oil.

15 30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspension seed-coating agent of embodiment

Biphenyl pyrazine bacterium amine 3%, amine benzene pyrrole bacterium ketone 27%, fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 10% are modified Calcium lignosulfonate 5%, xanthan gum 1%, bentonite 1%.Glycerine 5%, PVP-K30 1%, complements to 100%, by above-mentioned each component It mixes in proportion, and is sanded, be prepared into 30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone suspension seed-coating agent.

16 30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone granule of embodiment

Biphenyl pyrazine bacterium amine 2.5%, amine benzene pyrrole bacterium ketone 27.5%, polyethylene glycol 3%, the silicic acid 1% of high degree of dispersion, calcium carbonate is supplied To 100%, in a mixer, by levigate active component even spread to the carrier soaked by polyethylene glycol, by this method may be used Obtain 30% biphenyl pyrazine bacterium amine amine benzene pyrrole bacterium ketone granule.

Biphenyl pyrazine bacterium amine 5% and amine benzene pyrrole bacterium ketone 95% are uniformly mixed by embodiment 17.

Biphenyl pyrazine bacterium amine 10% and amine benzene pyrrole bacterium ketone 90% are uniformly mixed by embodiment 18.

Above embodiments proportion is weight per distribution ratio.

Biological test example:

Pesticide is made in the combination of active principles of different pesticides, is current exploitation and development novel pesticide and prevention and treatment agriculturally resistance A kind of effective and quick way of germ.After the pestsides synthesis of different cultivars, three kinds of type of action are typically exhibited: being added and make With, synergistic effect and antagonism.But it is specially which kind of effect, it is unpredictable, only just it can know that by many experiments.Compounding Synergy is formulated well, due to significantly improving practical control efficiency, the usage amount of pesticide is reduced, to greatly delay The generation of resistance.

One, virulence is tested:

The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture are calculated according to Sun Yunpei's Method, when CTC≤80, are then combined Object shows antagonism, and when 80<CTC<120, then composition shows summation action, and when CTC>=120, then composition shows Synergistic effect out.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

B in the percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture Percentage composition

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100

Test 1: the toxicity test of powdery mildew of cucumber

It is spraying under 50PSI pressure with potter spray tower selected from the consistent cucumber seedling of growing way, every basin about 5mL, each medicine 12 concentration gradients are arranged in agent.Bacterium is connect after chemicals treatment for 24 hours, the powdery mildew of cucumber leaf for picking up from field is uniform above cucumber seedling Shake off conidium to be inoculated with, then cucumber seedling is put into greenhouse and is cultivated.It is classified after 7d according to the morbidity of powdery mildew of cucumber Standard complete stool investigates disease index, and calculates control efficiency, concentration EC50 in inhibiting then is calculated with least square method, then according to grandson Yun Peifa calculates co-toxicity coefficient (CTC).

Table 1

As known from Table 1, the weight proportion of prevention powdery mildew of cucumber, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1:50's When in range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.

Test 2: the toxicity test of graw mold of tomato

Selected from the consistent tomato seedling of growing way, each processing selects 3 basins for trying leaf seedling, with potter spray tower under 50PSI pressure Spraying, 12 concentration gradients are arranged in every basin about 5mL, each medicament.Bacterium is connect after chemicals treatment for 24 hours, the tomato in field will be picked up from Gray mold leaf is uniformly shaken off conidium above tomato seedling and is inoculated with, and then tomato seedling is put into greenhouse and is cultivated.After 7d Disease index is investigated according to the morbidity grade scale complete stool of graw mold of tomato, and calculates control efficiency, then uses least square method Concentration EC50 in inhibiting is calculated, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 2: virulence test result of the present invention on prevention and treatment graw mold of tomato

As known from Table 2, the weight proportion of prevention graw mold of tomato, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1:50's When in range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.

Test 3: the toxicity test of marssonina coronaria

Using inhibition mycelial growth rate method:

Biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The biphenyl pyrazine bacterium amine of different ratio and amine benzene pyrrole bacterium ketone series of concentrations compound solution are made with same method Toxic culture medium.2 days marssonina coronarias will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 3: virulence test result of the present invention to marssonina coronaria

As known from Table 3, the weight proportion of prevention and treatment marssonina coronaria, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1:50 Range in when, co-toxicity coefficient is all larger than 120, both illustrates to show as synergistic effect within this range.

Test 4: the toxicity test of barley powdery mildew bacteria

Using inhibition mycelial growth rate method:

Biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The biphenyl pyrazine bacterium amine of different ratio and amine benzene pyrrole bacterium ketone series of concentrations compound solution are made with same method Toxic culture medium.2 days barley powdery mildew bacterias will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 4: virulence test result of the present invention to barley powdery mildew bacteria

As known from Table 4, the weight proportion of prevention and treatment barley powdery mildew bacteria, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1:50 Range in when, co-toxicity coefficient is all larger than 120, both illustrates to show as synergistic effect within this range.

Test 5: the toxicity test of wheat leaf rust germ

Using inhibition mycelial growth rate method:

Biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The biphenyl pyrazine bacterium amine of different ratio and amine benzene pyrrole bacterium ketone series of concentrations compound solution are made with same method Toxic culture medium.2 days wheat leaf rust germs will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with connecing Fungus block is moved to the toxic PDA culture medium center being configured in advance by kind needle, is subsequently placed in culture in 25 DEG C of incubators, every processing weight It is 4 times multiple.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each Two diameters are surveyed in bacterium colony right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 5: virulence test result of the present invention to wheat leaf rust germ

As known from Table 5, the weight proportion of prevention and treatment wheat leaf rust germ, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1: When in 50 range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.

Test 6: the toxicity test of apple tree Streptomyces scabies

Using inhibition mycelial growth rate method:

Biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The biphenyl pyrazine bacterium amine of different ratio and amine benzene pyrrole bacterium ketone series of concentrations compound solution are made with same method Toxic culture medium.2 days apple tree Streptomyces scabies will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with connecing Fungus block is moved to the toxic PDA culture medium center being configured in advance by kind needle, is subsequently placed in culture in 25 DEG C of incubators, every processing weight It is 4 times multiple.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each Two diameters are surveyed in bacterium colony right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 6: virulence test result of the present invention to apple tree Streptomyces scabies

As known from Table 6, the weight proportion of prevention and treatment apple tree Streptomyces scabies, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1: When in 50 range, co-toxicity coefficient is all larger than 120, illustrates that the two shows as synergistic effect within this range.

Test 7: the toxicity test of Sclerotinia sclerotiorum

Using inhibition mycelial growth rate method:

Biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The biphenyl pyrazine bacterium amine of different ratio and amine benzene pyrrole bacterium ketone series of concentrations compound solution are made with same method Toxic culture medium.2 days Sclerotinia sclerotiorums will be cultivated, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 7: virulence test result of the present invention to Sclerotinia sclerotiorum

As known from Table 7, the weight proportion of prevention and treatment Sclerotinia sclerotiorum, biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone is 50:1-1:50 Range in when, co-toxicity coefficient is all larger than 120, both illustrates to show as synergistic effect within this range.

Claims (10)

1. a kind of bactericidal composition, it is characterised in that: the bactericidal composition includes active constituent biphenyl pyrazine bacterium amine and amine benzene The weight proportion of pyrrole bacterium ketone, the biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone be 50:1-1:50, preferably 20:1-1:40, preferably 10:1-1:40, more preferable 8:1-1:35；More preferably 4:1-1:35.

2. bactericidal composition according to claim 1, it is characterised in that: the biphenyl pyrazine bacterium amine and amine benzene pyrrole bacterium ketone matter The sum of amount accounts for 5%-90%, more preferable 5%-80%, more preferable 10%-75%, the more preferable 10%-70% of the Bactericidal composite amount of substance, More preferable 10%-65%, more preferable 10%-60%, more preferable 10%-55%, more preferable 10%-50%.

3. bactericidal composition according to claim 1, it is characterised in that: the dosage form of the bactericidal composition is aqueous suspension Agent, seed treatment suspending agent, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspending agent, granule, missible oil, microemulsion, Aqueous emulsion, effervescent tablet, ultra low volume liquids, thermal fog, dry suspending agent, oil-suspending agent.

4. bactericidal composition according to claim 1, it is characterised in that: also include filler and/or surfactant.

5. bactericidal composition according to claim 1 is for preventing and treating cereal, bean, fruit tree and fruits, vegetable Vegetables, root vegetables, forage grass class, turfgrass or turf, spice crop, on flower plant fungi and bacterium purposes.

6. bactericidal composition according to claim 1 be used to prevent and treat brown spot on apple, spot defoliation, ring spot, Anthracnose, shot hole；Powdery mildew, the gray mold of more crops；Rice sheath blight disease, rice blast；Cabbage Leaf Spot；Lawn giant spot disease； Wheat leaf rust；Early blight of tomato；The purposes of sclerotinia sclerotiorum.

7. bactericidal composition according to claim 1 is used to handle seed to protect seed from fungi and bacteria attack Purposes.

8. bactericidal composition according to claim 1 is used to handle the soil of plant growth to protect the plants from soil Fungi and bacteria attack purposes.

9. a kind of method for preventing and treating plant pathogen, which is characterized in that will power before plant is by disease infestation or after infecting Benefit require 1 described in bactericidal composition act on plant, plant propagation material and the plant organ then grown, cultivation medium, In material or space.

10. according to the method described in claim 9, it is characterized by: bactericidal composition described in claim 1 is had with agronomy The amount of application of effect and basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instiled, be poured, spray, sprayed The methods of mist, dusting, distribution or smoke be administered to plant, plant propagation material and the plant organ then grown, cultivation medium, In material or space.