CN109042700A - A kind of combination sterilization composition for preventing and treating grape grey mould - Google Patents
A kind of combination sterilization composition for preventing and treating grape grey mould Download PDFInfo
- Publication number
- CN109042700A CN109042700A CN201810950201.6A CN201810950201A CN109042700A CN 109042700 A CN109042700 A CN 109042700A CN 201810950201 A CN201810950201 A CN 201810950201A CN 109042700 A CN109042700 A CN 109042700A
- Authority
- CN
- China
- Prior art keywords
- agent
- fludioxonil
- active constituent
- rosickyite azoles
- grey mould
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The combination sterilization composition of prevention and treatment grape grey mould disclosed by the invention, the combination sterilization composition is aqueous suspension agent, including active constituent and auxiliary agent, wherein active constituent is to be compounded with rosickyite azoles with fludioxonil base-material, and auxiliary agent includes: wetting dispersing agent, thickener, antifreezing agent and defoaming agent, the mass ratio of various composition is as follows: active constituent 5%~50%, wetting dispersing agent 2%~6%, thickener 0.1%~0.4%, antifreezing agent 3%~5%, defoaming agent 0.4%~0.6%, adds water to 100%.The quality proportioning of rosickyite azoles and fludioxonil is 1: 4~4: 1 in the active constituent.The combination sterilization composition of prevention and treatment grape grey mould of the invention, after two kinds of base-materials compound, effect is obviously added, and use is safe, has good cytotoxicity for grape grey mould germ, and be apparently higher than rosickyite azoles and fludioxonil single dose.Suitable for vast town and country for preventing and treating grape grey mould.
Description
Technical field
The present invention is related with fungicide, specifically, belonging to the fungicide containing organic compound, furthermore, it is understood that belonging to
The bactericidal composition for preventing and treating grape grey mould.
Technical background is current
Grape Mild Powder (Grape gray mold), it is commonly called as " etching fringe ", pathogen is Botrytis cinerea.Grape grey mould is mesh
A kind of more serious disease occurs on former world, in the disease that all storages occur.Inflorescence, young fruit are susceptible, first in bennet
With generate filbert, water soaking mode scab on small carpopodium or cob, rear scab browning color and soft corruption can on scab when there was dampness in the air
Generate Slate grey mustiness object, the i.e. conidiophore and conidium of pathogen.When being air-dried, susceptible inflorescence, young fruit by
Gradually dehydration, atrophy, it is rear withered to fall off, cause a large amount of fruit drop, when serious, can whole fringe fall light.Young sprout and spire are susceptible, produce
Raw filbert or bronzing, irregular scab, scab are mostly occurring at vein, are occurring sometimes on blade distant
Line is taken turns, also may occur in which grey mould layer when the later period, there was dampness in the air on scab.Substantial young sprout is not in season of growth late onset, skin zone
In bleaching color, there is black sclerotium or form the grey mycelia block of spore.It is susceptible after fruit starching, occur brown recess disease on fruit face
Spot, after extension, entire fruit rot, and first at pericarp crack generate grey sorus, after spread to entire fruit, finally grow
The mould layer of grey out.Sometimes it can produce the mycelia block of black sclerotium or grey in sick portion.
Rosickyite azoles also known as albendazole, (English is entitledAlbendazole, molecular formula is C12H15N3O2S, molecular weight are
265.33) be a kind of people's insecticide active constituent, 2012, Guizhou road combined Beijing of member biology plant pesticide research
University of TCM, Agricultural University Of Nanjing and beneficial one hundred pharmacy Miao Yao R&D team successfully develop the innovative green bactericide of rosickyite azoles,
The fungicide mixed morbidities a variety of to plant are harmful and pathogenic infection has inhibiting effect, belong to the agriculture of absorbability rosickyite azole a new generation
With fungicide, there is prevention & protection, interior suction treatment, infiltration conduction.The fungicide wide sterilization spectrum has stronger effect side
The complementarity of formula and bioactivity is significant with the effect of other composite reagent synergisms.For in efficient, less toxic, real wide spectrum
Absorption fungicide, it may have the feature that activity is high, the lasting period is long, absorbability is strong, spraying time is flexible has brown spot preferably anti-
Effect.C12H6N2O2
Fludioxonil (English namefludioxonil, molecular formula is C12H6 F2N2O2, molecular weight 248.18) and it is a kind of contact toxicity
Fungicide is used for seed treatment, can prevent and treat the fungal disease of seed-borne fungi and soil-borne.Lasting period is long, and be not easy with it is other
Fungicide occurs cross resistance and is used for wheat, barley, corn, pea, rape, rice, vegetables, grape, lawn, ornamental crops leaf
Surface treatment, prevention and treatment avenge rotten Fusariumsp, wheat net raw meat black rot, rhizoctonia solani etc., have special efficacy to gray mold；To cereal and non-cereal
Seed treatment, prevention and treatment kind passes and soil-borne disease bacterium, such as Alternaria, Ascochyta, aspergillus, Fusarium, Helminthosporium, silk core
Pseudomonas and Penicillium bacterium, have special efficacy to grape grey mould.
The application part for preventing and treating grape Mild Powder involved in Chinese patent database has: ZL 201310299766X is " a kind of
Control method before the blooming of grape grey mould ", ZL 2014100969373 " a kind of biological control method of grape grey mould ",
No. 2015103741058 " a kind of grape grey mould control method ", No. 2016111831493 " pterostilbene is in prevention and treatment grape grey mold
Disease and peronophythora litchi in application ", No. 201710070581X " a kind of control method of grape grey mould ",
No. 2018101054740 " resveratrol is preventing and treating the application in grape grey mould as the synergetic effect additive of pyrimethanil " etc..Mesh
Before there is no compounded with rosickyite azoles with fludioxonil prevention and treatment grape Mild Powder bactericidal composition application part.
Summary of the invention
The present invention is intended to provide a kind of combination sterilization composition for preventing and treating grape grey mould, it is with rosickyite azoles and fludioxonil
The bactericidal composition of compounding, to improve yield of strawberry and quality.
Inventor carries out repeated screening to the pesticide that gray mold has favorable effect to having, and to the bulk pharmaceutical chemicals of compound composition
Quality proportioning carries out repetition test, compares to test result, finds a kind of compound disinfectant, have synergistic effect, to Portugal
The stronger poisoning ability of the germ of grape gray mold.
The combination sterilization composition for the prevention and treatment grape grey mould that inventor provides is aqueous suspension agent, including active constituent with help
Agent, wherein active constituent is compounded using rosickyite azoles with fludioxonil as base-material, auxiliary agent include: wetting dispersing agent, thickener,
Antifreezing agent and defoaming agent；
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Defoaming agent 0.4%~0.6%
Water adds to 100%；
The quality proportioning of rosickyite azoles and fludioxonil is 1: 4~4: 1 in the active constituent；The rosickyite azoles is mass fraction 98%
Raw medicine, fludioxonil be mass fraction 95% raw medicine.
Preferably, the active constituent is that rosickyite azoles compounds to obtain with fludioxonil according to quality proportioning 1: 1.
In the auxiliary agent, wetting dispersing agent is agriculture breast 600-1, and thickener is xanthan gum, and antifreezing agent is ethylene glycol, defoaming
Agent is methanol.
According to experimental result, inventor is determined, with the prevention and treatment grape grey mould for the bactericidal composition pair that said ratio is prepared
With synergistic effect.
The Bioexperiment for preventing and treating the combination sterilization composition of grape grey mould is as follows:
Rosickyite azoles used is produced by Guizhou Daoyuan Biotechnology Co., Ltd.；Fludioxonil used is by Zhejiang standing grain Ordering-the Science and Technology Ltd.
Production.
Toxicity measures
1.1 Study on Fungicide Toxicity
Referring to farm-chemical indoor determination test rule, using mycelial growth rate method is used, measurement is for examination single dose to light grey and pinkish
The inhibiting rate of mildew.Using mycelial growth rate method, the PDA culture medium that 0.5ml medical fluid and 4.5ml melt is mixed, and is poured into sterile
Culture dish in be made band medicine culture medium flat plate.After culture medium solidification, 1 bacteria cake for trying bacterium is put into each culture base plane
(diameter 0.5cm) makes bacteria cake be attached to media surface on one side with mycelia, and 3 repetitions of every processing with alcohol solvent and contain
0.1% Tween 80 aqueous solution is control.After cultivating 72h at 25 DEG C, with crossing method measurement for trying bacterium colony growth diameter.
Inhibiting rate is calculated according to the following formula.And rosickyite azoles, the toxicity regression curve of fludioxonil single dose and EC50 are found out by probit analysis
Value, SD and 95% confidence limit.
Mycelial growth inhibition rate (%)=(control colony growth diameter-processing colony growth diameter)/control bacterium colony growth is straight
Diameter × 100
The research of the complex role of 1.2 rosickyite azoles and fludioxonil
Rosickyite azoles and fludioxonil are compounded: two kinds of raw medicines are configured to 4: 1,3: 1,2: 1,1: 1,1: 2,1 by effective component:
3,1:4 totally 7 quality proportionings.Above-mentioned formula presses Study on Fungicide Toxicity method respectively and carries out toxicity test.
Mixture synergy is evaluated, with EC50Based on value, mixture joint is evaluated by calculating mixture co-toxicity coefficient
Type of action.
CTC120 be synergistic effect, and 80≤CTC < 120 are summation action, and CTC < 80 are antagonism.
Test result
2.1 Study on Fungicide Toxicity results
The Toxicity Determination result of the 12 kinds of medicaments of table to grape grey mould
| Treatment agent | Virulence regression equation | EC50(mgL–1) | Related coefficient | SD | CL0.95 |
| Rosickyite azoles | y = 0.9764x + 2.9274 | 131.6454 | 0.9790 | 0.2879 | 35.9050~482.6774 |
| Fludioxonil | y = 0.7949x + 3.4971 | 77.8372 | 0.9800 | 0.3405 | 16.7390~361.9473 |
As it can be seen from table 1 EC of the rosickyite azoles to grape grey mould50For 131.6454 μ g/mL；Fludioxonil is to grape grey mould
EC50For 77.8372 μ g/mL.
2 rosickyite azoles of table compounds the The Toxicity Determination result to grape grey mould with fludioxonil
| Rosickyite azoles: fludioxonil | Virulence regression equation | Related coefficient | EC50(μ g/mL) | Co-toxicity coefficient |
| 1∶4 | y = 0.9596x + 3.4468 | 0.9881 | 41.3338 | 205.0781 |
| 1∶3 | y = 1.2981x + 2.9192 | 0.9985 | 40.2434 | 215.4296 |
| 1∶2 | y = 0.7065x + 3.8639 | 0.9659 | 40.3918 | 223.1018 |
| 1∶1 | y = 1.0357x + 3.3456 | 0.9760 | 39.4995 | 247.6756 |
| 2∶1 | y = 1.1792x + 2.9121 | 0.9677 | 57.8004 | 185.1049 |
| 3∶1 | y = 0.7711x + 3.6791 | 0.9056 | 50.6557 | 221.5873 |
| 4∶1 | y = 1.0148x + 3.2858 | 0.9751 | 49.2839 | 234.6714 |
Interpretation of result: as shown in Table 2, all proportions of rosickyite azoles and fludioxonil compounding show certain synergistic effect, but
Different compound proportion coefficient of synergism difference.Wherein, when the compound proportion of rosickyite azoles and fludioxonil is 1: 1, co-toxicity coefficient is most
Height is 247.6756.
The combination sterilization composition of prevention and treatment grape grey mould of the invention, after two kinds of base-materials compound, effect is obviously added, and is made
With safety, there is good cytotoxicity for grape grey mould germ, and is higher than rosickyite azoles and ZEN 90160 single dose.Suitable for wide
Big town and country are for preventing and treating grape grey mould.
Specific embodiment
Embodiment 1
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd. to produce is 98% rosickyite azoles raw medicine 3.0kg, then takes Zhejiang
The fludioxonil raw medicine 3.0kg of the mass fraction 95% of standing grain Ordering-the Science and Technology Ltd. production is re-dubbed 6.0 kg prevention and treatment grape grey mold
The base-material of the combination sterilization composition of disease；
0.4 kg of wetting dispersing agent agriculture breast 600-1,0.02 kg of thickener xanthan gum are taken again, and antifreezing agent is ethylene glycol 0.4
Kg, 0.05 kg of defoaming agent methanol add water until 10kg, obtains the combination sterilization composition 10kg for controlling apple anthrax.
Embodiment 2
The mass fraction for taking Guizhou Daoyuan Biotechnology Co., Ltd. to produce is 98% rosickyite azoles raw medicine 4.0kg, then takes Zhejiang
The fludioxonil raw medicine 1.0kg of the mass fraction 50% of standing grain Ordering-the Science and Technology Ltd. production is re-dubbed 5.0 kg prevention and treatment grape grey mold
The base-material of the combination sterilization composition of disease；0.3 kg of wetting dispersing agent agriculture breast 600-1, thickener xanthan gum 0.02 are taken again
Kg, antifreezing agent are 0.4 kg of ethylene glycol, 0.05 kg of defoaming agent methanol, add water until 10kg, obtains answering for prevention and treatment grape grey mould
With bactericidal composition 10kg.
Rosickyite azoles and the bactericidal composition of fludioxonil of a kind of prevention and treatment grape grey mould of the invention and application thereof have passed through
Specific embodiment is described.Those skilled in the art can use for reference the appropriate feed change of the contents of the present invention, technique item
The links such as part realize that corresponding other purposes, correlation change all without departing from the contents of the present invention, all similar replacements
With change it will become apparent to those skilled in the art that being considered as being included within the scope of the present invention.
Claims (3)
1. a kind of combination sterilization composition for preventing and treating grape grey mould, it is characterised in that the composition is aqueous suspension agent, including work
Property ingredient and auxiliary agent, wherein active constituent is to be compounded using rosickyite azoles with fludioxonil as base-material, and auxiliary agent includes: wetting and dispersing
Agent, thickener, antifreezing agent and defoaming agent；
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Defoaming agent 0.4%~0.6%
Water adds to 100%；
The quality proportioning of rosickyite azoles and fludioxonil is 1: 4~4: 1 in the active constituent；The rosickyite azoles is mass fraction 98%
Raw medicine, fludioxonil be mass fraction 95% raw medicine.
2. combination sterilization composition as described in claim 1, it is characterised in that the combination sterilization composition is aqueous suspension agent, packet
Active constituent and auxiliary agent are included, wherein active constituent is compounded using rosickyite azoles with ZEN 90160 as base-material, and auxiliary agent includes: profit
Hygroscopic water powder, thickener, antifreezing agent and defoaming agent；
The mass ratio of various composition is as follows:
Active constituent 5%~50%
Wetting dispersing agent 2%~6%
Thickener 0.1%~0.4%,
Antifreezing agent 3%~5%
Defoaming agent 0.4%~0.6%
Water adds to 100%；
The quality proportioning of rosickyite azoles and fludioxonil is 1: 1 in the active constituent；The rosickyite azoles is the original of mass fraction 98%
Medicine, fludioxonil are the raw medicine of mass fraction 95%.
3. combination sterilization composition as described in claim 1, it is characterised in that in the auxiliary agent, wetting dispersing agent is agriculture breast
600-1, thickener are xanthan gum, and antifreezing agent is ethylene glycol, and defoaming agent is methanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810950201.6A CN109042700A (en) | 2018-08-20 | 2018-08-20 | A kind of combination sterilization composition for preventing and treating grape grey mould |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810950201.6A CN109042700A (en) | 2018-08-20 | 2018-08-20 | A kind of combination sterilization composition for preventing and treating grape grey mould |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109042700A true CN109042700A (en) | 2018-12-21 |
Family
ID=64687567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810950201.6A Pending CN109042700A (en) | 2018-08-20 | 2018-08-20 | A kind of combination sterilization composition for preventing and treating grape grey mould |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109042700A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632795A (en) * | 2021-05-07 | 2021-11-12 | 广东省农业科学院果树研究所 | Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258042A (en) * | 2010-05-27 | 2011-11-30 | 青岛奥迪斯生物科技有限公司 | Germicide composition containing fludioxonil and thiophanate-methyl |
| CN104904723A (en) * | 2014-03-11 | 2015-09-16 | 江苏龙灯化学有限公司 | Sterilization composition |
| CN105076169A (en) * | 2015-09-09 | 2015-11-25 | 江苏省绿盾植保农药实验有限公司 | Bactericidal composition containing albendazole and fludioxonil and application |
-
2018
- 2018-08-20 CN CN201810950201.6A patent/CN109042700A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102258042A (en) * | 2010-05-27 | 2011-11-30 | 青岛奥迪斯生物科技有限公司 | Germicide composition containing fludioxonil and thiophanate-methyl |
| CN104904723A (en) * | 2014-03-11 | 2015-09-16 | 江苏龙灯化学有限公司 | Sterilization composition |
| CN105076169A (en) * | 2015-09-09 | 2015-11-25 | 江苏省绿盾植保农药实验有限公司 | Bactericidal composition containing albendazole and fludioxonil and application |
Non-Patent Citations (1)
| Title |
|---|
| 张淼等主编: "《同药异名速查手册》", 31 August 2014, 人民军医出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113632795A (en) * | 2021-05-07 | 2021-11-12 | 广东省农业科学院果树研究所 | Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103314968B (en) | Bactericidal composition with synergistic effect | |
| CN101642126A (en) | Compound bactericidal composition containing fludioxonil | |
| CN104322558A (en) | Sterilization composition and applications thereof | |
| CN106973907A (en) | A kind of composition pesticide containing copper 8-hydroxyquinolinate and kasugarnycin and its application | |
| CN103444729A (en) | Bactericidal pharmaceutical composition and applications thereof | |
| CN103039468A (en) | Oligochitosan-propineb composition used for plant protection | |
| CN109548791A (en) | A kind of bactericidal composition | |
| CN104106580B (en) | A kind of composite bactericide preventing and treating wheat scab | |
| CN109042700A (en) | A kind of combination sterilization composition for preventing and treating grape grey mould | |
| CN105028435B (en) | A kind of native compound is as the purposes of agricultural bacteriocide | |
| CN101653131A (en) | Bactericidal composition containing azoxystrobin | |
| CN106857511A (en) | A kind of microbicide compositions comprising emodin derivates Yu pyrazol acid amide compounds | |
| CN101438703B (en) | Sterilizing compositional composition containing eugenol | |
| CN103858874B (en) | Application of natural compound as agricultural bactericide | |
| CN109042701A (en) | A kind of rosickyite azoles and the bactericidal composition of trifloxystrobin and application thereof for preventing and treating Cucumber Target Leaf Spot | |
| CN105707106A (en) | Bactericidal composition | |
| CN104115841A (en) | Fungicide composition containing fludioxonil and application thereof | |
| CN110495456A (en) | A kind of bactericidal composition | |
| CN107333792A (en) | By rosickyite azoles and the bactericide of the preventing and treating rice blast of miaow chaff azoles compounding | |
| CN108338165A (en) | A kind of microbicide compositions including emodin derivates and polysaccharide | |
| CN108617676A (en) | A kind of combination sterilization composition of prevention lawn reaping hook droop | |
| CN106889091A (en) | A kind of composite bactericide for preventing and treating sigatoka | |
| CN108925573A (en) | A kind of combination sterilization composition for preventing and treating Kiwi berry soft rot | |
| CN101731221B (en) | Sterilizing composition | |
| CN105191997A (en) | Fungicide containing prochloraz and scutellaria baicalensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181221 |