CN104106580B - A kind of composite bactericide preventing and treating wheat scab - Google Patents
A kind of composite bactericide preventing and treating wheat scab Download PDFInfo
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- CN104106580B CN104106580B CN201410241810.6A CN201410241810A CN104106580B CN 104106580 B CN104106580 B CN 104106580B CN 201410241810 A CN201410241810 A CN 201410241810A CN 104106580 B CN104106580 B CN 104106580B
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- albendazole
- tebuconazole
- wheat scab
- compound disinfectant
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Abstract
The invention discloses a kind of compound disinfectant preventing and treating wheat scab, this compound disinfectant is with albendazole and Tebuconazole for base-material, formulated according to the quality proportioning of albendazole and Tebuconazole 7: 1 ~ 1: 7.Preferred albendazole and Tebuconazole quality proportioning are 1: 2.Albendazole is the bactericide original drug of mass fraction 97%, and Tebuconazole is the bactericide original drug of mass fraction 98.43%.Compound disinfectant of the present invention, there is not chemical reaction in two kinds of base-materials, has obvious synergy or be added effect, use safety, good cytotoxicity is had for the fusarium graminearum that current field is dissimilar, and apparently higher than albendazole or Tebuconazole single dose.Be applicable to vast rural area for preventing and treating wheat scab.
Description
Technical field
The present invention is relevant with bactericide, specifically, belongs to the bactericide containing organic compound, furthermore, belongs to the bactericide preventing and treating wheat scab.
Technical background
Wheat scab formal name used at school F.graminearum schw (FusaHumgraminearumSehw), withered, the rotten wheat head of the another name wheat head, red wheat head are one of Major Diseases of wheat.Wheat scab generally occurs in the whole world, and be mainly distributed in moist and semi-humid region, especially the moistening rainy Temperate Region in China of weather is injured seriously.All can be injured to heading from seedling, mainly cause that seedling is withered, brown foot rot, stalk are rotten and fringe is rotten, the most seriously fringe of wherein causing harm is rotten.Wheat scab is caused by multiple sickle-like bacteria, and these germs are collectively referred to as fusarium graminearum [Gibbreliazeae (Schw.) Petch].
Albendazole and Albendazole (Albendazole), be a kind of efficient, safety, broad spectrum activity imidazoles bactericide, have significant preventive effect, and have certain production-increasing function to plurality of plant diseases, but undesirable to preventing and treating wheat scab effect; Tebuconazole (Tebuconazole) is a kind of efficient, wide spectrum, interior absorption triazole type sterilization pesticide, but undesirable to wheat scab control efficiency, also has certain inhibitory action to wheat growth.It is all undesirable that wheat scab effect prevented and treated respectively by these two kinds of agricultural chemicals.
In recent years, the problem of preventing and treating wheat scab is subject to people's attention, develop the bactericide that some are relevant, such as No. 00112638.5, Chinese patent application part " preventing and treating the vegetable active oligosaccharide agricultural chemicals of the crop pests such as wheat scab ", No. 200810022910.4 " a kind of plant medical agent prevents and treats the compound method of wheat scab ", No. 201010202322.6 " a kind of bactericide preventing and treating wheat scab " and No. 201110332967.6 " a kind of chitosan oligosaccharide composition for anti-wheat scab and uses thereof and methods " etc.How these fungicide effects are still not clear.There is no the related application part with albendazole and the composite bactericide to fusarium graminearum of Tebuconazole at present.
Summary of the invention
The object of the present invention is to provide a kind of compound disinfectant preventing and treating wheat scab, it possesses stronger poisoning ability to fusarium graminearum, can be applied to vast rural area and prevent and treat wheat scab, the wheat increase yield accounting for agricultural product critical role is increased income, be the development service of national economy.
Inventor carries out repeated screening to having the agricultural chemicals killing fusarium graminearum activity, and repetition test is carried out to the bulk drug quality proportioning of Mixed Pharmacy, result of the test is contrasted, finds a kind of compound disinfectant, stronger poisoning ability is possessed to fusarium graminearum.
The compound disinfectant of wheat scab that what inventor provided prevent and treat is with albendazole and Tebuconazole for base-material, according to the compound disinfectant that the quality proportioning 7: 1 ~ 1: 7 of albendazole and Tebuconazole is formulated.
Through preferred further, the best in quality proportioning of albendazole and Tebuconazole is 1: 2.
Above-mentioned albendazole is the bactericide original drug of mass fraction 98.9%, and above-mentioned Tebuconazole is the bactericide original drug of mass fraction 97.5%.
Above-mentioned screening test of preventing and treating the compound disinfectant of wheat scab is as follows:
1 test objective: take fusarium graminearum as target, adopts mycelial growth rate method, measures the optimal proportion that albendazole Tebuconazole is composite.
2 experimental conditions
2.1 for examination target
Fusarium graminearum [Gibbrellazeae (Schw.) Petch], gathers from Zhejiang, is separated qualification; 4 DEG C of Refrigerator stores.
2.2 condition of culture
Transfer after 1 time on PDA culture medium flat plate before qualification, preculture 4d at 25 DEG C, is used for mensuration from colony edge cut-off footpath 5mm mycelia block.
3 experimental scheme
3.1 test medicine
(1) the former medicine of albendazole Ultramicro-powder, effective content 98.9%, is provided by Guizhou Daoyuan Biotechnology Co., Ltd..
(2) Original Tebuconazole, effective content 97.5%, by Changzhou, bumper harvest agrochemical Co., Ltd provides;
3.2 test process
3.2.1 dosage is arranged
Reagent agent albendazole DMF is made into 10000 μ g/ml mother liquors; Tebuconazole methyl alcohol is made into 10000 μ g/ml mother liquors.Albendazole, Tebuconazole are composite with 7: 1,5: 1,3: 1,1: 1,1: 2,1: 3,1: 5,1: 7 eight ratio respectively.Medicament joins in AEA medium by series concentration designed after preliminary experiment respectively and makes pastille flat board.The mensuration concentration of albendazole is 100,50,25,12.5,6.25 μ g/ml; The mensuration concentration of Tebuconazole is 2.5,1.25,0.625,0.3125,0.0156 μ g/ml.The mensuration concentration of albendazole Tebuconazole built agent is in Table.If without the contrast of medicine flat board.
3.2.2 repetition is tested
Each concentration process repeats 4 wares.
4 test methods
Adopt mycelial growth rate method.The previously prepared inoculated by hypha block of picking is on the PDA culture medium flat plate of different agents concentration, 4d is cultivated at 25 DEG C, check colony diameter, calculate the percentage that each chemicals treatment suppresses mycelial growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of serial drug concentration, obtain the EC of each medicament
50, calculate the synergistic effect of mixed agent according to the method for Wadley, i.e. SR<0.5, then two kinds of medicament mixtures have antagonism, 0.5≤SR≤1.5, then two kinds of medicament mixtures have summation action, SR>1.5, then two kinds of medicament mixtures have synergistic effect.
SR＝X
1/X
2
5 toxicity tests
Albendazole and Tebuconazole are tested by one's own efforts according to above-mentioned 8 proportionings, and result is as follows
8 composite proportionings of albendazole and Tebuconazole are to the synergy evaluation of wheat scab
6 interpretations of result
Albendazole suppresses the EC of fusarium graminearum mycelial growth
50value is 13.5142 μ g/ml; Tebuconazole suppresses the EC of fusarium graminearum mycelial growth
50value is 0.8579 μ g/ml.Albendazole Tebuconazole in 7: 1,5: 1,3: 1,1: 1,1: 2,1: 3,1: 5,1: 7 ratio composite after, EC
50value and SR
50value sees the above table, the synergistic effect of all doing well; Best in quality proportioning is 1: 2.
Compound disinfectant of the present invention, there is not chemical reaction in two kinds of base-materials, has obvious synergy or be added effect, use safety, good cytotoxicity is had for the fusarium graminearum that current field is dissimilar, and apparently higher than albendazole or Tebuconazole single dose.Be applicable to vast rural area for preventing and treating wheat scab.
Embodiment
Embodiment: the albendazole 2.5kg getting the mass fraction 98.9% that Guizhou Daoyuan Biotechnology Co., Ltd. produces, be base-material with the Tebuconazole 5.0kg of mass fraction 97.5% that Changzhou bumper harvest agrochemical Co., Ltd produces, be re-dubbed the compound disinfectant that 7.5kg prevents and treats wheat scab.
This compound disinfectant uses in somewhere, Guizhou, obtains treatment wheat scab remarkable result.
Claims (3)
1. prevent and treat a compound disinfectant for wheat scab, it is characterized in that this compound disinfectant is with albendazole and Tebuconazole for base-material, formulated according to the quality proportioning 7: 1 ~ 1: 7 of albendazole and Tebuconazole.
2., according to compound disinfectant according to claim 1, it is characterized in that this compound disinfectant is with albendazole and Tebuconazole for base-material, formulated according to the quality proportioning of albendazole and Tebuconazole 1: 2.
3., according to compound disinfectant according to claim 2, it is characterized in that described albendazole is the bactericide original drug of mass fraction 98.9%, described Tebuconazole is the bactericide original drug of mass fraction 97.5%.
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| CN201410241810.6A CN104106580B (en) | 2014-06-03 | 2014-06-03 | A kind of composite bactericide preventing and treating wheat scab |
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| CN104106580A CN104106580A (en) | 2014-10-22 |
| CN104106580B true CN104106580B (en) | 2016-01-06 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872155B (en) * | 2015-06-16 | 2016-09-21 | 贵州道元生物技术有限公司 | A kind of compound disinfectant preventing and treating rice blast |
| CN106359415A (en) * | 2016-08-29 | 2017-02-01 | 广东中迅农科股份有限公司 | Pesticidal composition containing albendazole and triazole fungicide |
| CN106857598A (en) * | 2017-03-02 | 2017-06-20 | 贵州道元生物技术有限公司 | A kind of compound disinfectant for preventing and treating Powdery Mildew in Tobacco |
| CN106818803A (en) * | 2017-03-02 | 2017-06-13 | 贵州道元生物技术有限公司 | The bactericide for preventing and treating wheat scab that rosickyite azoles is compounded with 2-cyano-3-amino-3-phenylancryic acetate |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101990911A (en) * | 2010-10-21 | 2011-03-30 | 贵州道元生物技术有限公司 | Medicine for preventing and treating ralstonia solanacearum |
| CN103329922A (en) * | 2013-07-17 | 2013-10-02 | 江苏省绿盾植保农药实验有限公司 | Compound sterilization composition, antiseptic and application of antiseptic |
| CN103444742A (en) * | 2013-09-24 | 2013-12-18 | 南京农业大学 | Sterilization composition containing metconazole and albendazole |
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2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990911A (en) * | 2010-10-21 | 2011-03-30 | 贵州道元生物技术有限公司 | Medicine for preventing and treating ralstonia solanacearum |
| CN103329922A (en) * | 2013-07-17 | 2013-10-02 | 江苏省绿盾植保农药实验有限公司 | Compound sterilization composition, antiseptic and application of antiseptic |
| CN103444742A (en) * | 2013-09-24 | 2013-12-18 | 南京农业大学 | Sterilization composition containing metconazole and albendazole |
Non-Patent Citations (1)
| Title |
|---|
| 戊唑醇与叶菌唑对小麦赤霉病的防治效果;韩青梅 等;《植物保护学报》;20031231;第30卷(第4期);第439-440页 * |
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