CN106818803A - The bactericide for preventing and treating wheat scab that rosickyite azoles is compounded with 2-cyano-3-amino-3-phenylancryic acetate - Google Patents
The bactericide for preventing and treating wheat scab that rosickyite azoles is compounded with 2-cyano-3-amino-3-phenylancryic acetate Download PDFInfo
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- CN106818803A CN106818803A CN201710119431.3A CN201710119431A CN106818803A CN 106818803 A CN106818803 A CN 106818803A CN 201710119431 A CN201710119431 A CN 201710119431A CN 106818803 A CN106818803 A CN 106818803A
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- Prior art keywords
- cyano
- amino
- phenylancryic acetate
- albendazole
- wheat scab
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
Abstract
The invention discloses a kind of compound disinfectant for preventing and treating wheat scab, the compound disinfectant is with rosickyite azoles and 2-cyano-3-amino-3-phenylancryic acetate as base-material, the compound disinfectant that quality proportioning 1: 5~1: 15 according to albendazole and 2-cyano-3-amino-3-phenylancryic acetate is formulated, the albendazole is the active compound of mass fraction 97%, and the 2-cyano-3-amino-3-phenylancryic acetate is 95% active compound.The quality proportioning of preferred albendazole and 2-cyano-3-amino-3-phenylancryic acetate is 1: 10.Compound disinfectant of the invention, two kinds of base-materials are not chemically reacted, and effect is added with obvious, using safety, have a good cytotoxicity for the different types of fusarium graminearum in current field, and effect is apparently higher than albendazole or 2-cyano-3-amino-3-phenylancryic acetate single dose.The built agent can also effectively prevent and treat the diseases such as base rot disease, root-rot and the marrow necrosis of the crops such as take-all, bakanae disease of rice, tomato, cucumber.It is used to prevent and treat wheat scab suitable for vast rural area.
Description
Technical field
The present invention is relevant with bactericide, specifically, belongs to the bactericide containing organic compound, furthermore, it is understood that belonging to
Prevent and treat the bactericide of wheat scab.
Technical background
Wheat scab scientific name F.graminearum schw (FusaHumgraminearum Sehw), the alias wheat head is withered, rotten wheat head, red
Wheat head, is one of Major Diseases of wheat.Wheat scab generally occurs in the whole world, is distributed mainly on moist and semi-humid area
It is aggrieved serious that domain, especially weather moisten rainy Temperate Region in China.All can be aggrieved to heading from seedling, mainly cause that seedling is withered, stem foot
Rotten, stalk is rotten and fringe is rotten, wherein the most serious that cause harm is rotten fringe.Wheat scab is caused by various sickle-like bacteria, and these germs are collectively referred to as
It is fusarium graminearum [Gibbrelia zeae (Schw.) Petch].
Albendazole is Albendazole (Albendazole), is a kind of efficient, safety, broad spectrum activity imidazoles series bactericidal agent,
There is significant preventive effect to plurality of plant diseases, and have certain production-increasing function, have certain effect to preventing and treating wheat scab effect；
2-cyano-3-amino-3-phenylancryic acetate (phenamacril), chemical name is 2- cyano group -3- amino -3- ethyl phenylacrylates, belongs to 2- alpha-cyanoacrylates
Esters bactericide.Active compound outward appearance is white solid powder；Brown foot rot, the root-rot of the crops such as tomato, cucumber can effectively be prevented and treated
With the disease such as marrow necrosis, its preventing and treating to wheat scab also needs to checking.
In recent years, the problem for preventing and treating wheat scab is valued by people, and develops some related bactericide, for example
Chinese patent application part 200810022910.4《A kind of plant medical agent prevents and treats the compound method of wheat scab》、
No. 201010202322.6《A kind of bactericide for preventing and treating wheat scab》With No. 201110332967.6《One kind is used for anti-wheat
Chitosan oligosaccharide composition of head blight and application thereof and method》Deng.There is no at present and compound preventing and treating wheat with rosickyite azoles and 2-cyano-3-amino-3-phenylancryic acetate
The application part of the bactericide of gibberellic hypha.
The content of the invention
The present invention is intended to provide the bactericide for preventing and treating wheat scab compounded with rosickyite azoles and 2-cyano-3-amino-3-phenylancryic acetate, it is to wheat
Gibberellic hypha possesses poisoning ability, can be applied to vast rural area and prevent and treat wheat scab, makes to account for the small of agricultural product critical role
Wheat increasing both production and income, is the development service of national economy.
Inventor screens to the agricultural chemicals for being possible to kill fusarium graminearum activity, and to the bulk drug of Mixed Pharmacy
Quality proportioning carries out repetition test, and result of the test is contrasted, and finds a kind of compound disinfectant, possesses to fusarium graminearum
Stronger poisoning ability.
Inventor provide the compound disinfectant for preventing and treating wheat scab be with rosickyite azoles and 2-cyano-3-amino-3-phenylancryic acetate as base-material, according to
The compound disinfectant that the quality proportioning 1: 5~1: 15 of albendazole and 2-cyano-3-amino-3-phenylancryic acetate is formulated.
By further preferred, the best in quality proportioning of albendazole and 2-cyano-3-amino-3-phenylancryic acetate is 1: 10.
Above-mentioned albendazole is the active compound of mass fraction 97%, and above-mentioned 2-cyano-3-amino-3-phenylancryic acetate is the active compound of mass fraction 95%.
The screening test of the above-mentioned compound disinfectant for preventing and treating wheat scab is as follows：
1 test objective
With fusarium graminearum as target, using mycelial growth rate method, albendazole 2-cyano-3-amino-3-phenylancryic acetate compounding is determined
Optimal proportion.
2 experimental conditions
2.1 for examination target
Fusarium graminearum [Gibbrella zeae (Schw.) Petch], from Zhejiang collection, separates identification；4 DEG C of refrigerators
Preserve.
2.2 condition of culture
In identification before on PDA culture medium flat board transfer 1 time after, at 25 DEG C culture.
2,3 reagent agents
97% albendazole active compound is provided by Guizhou Daoyuan Biotechnology Co., Ltd., 95% 2-cyano-3-amino-3-phenylancryic acetate active compound is by Jiangsu
Southern pesticide research center provides.
3 test doses are designed
3.1 experimental designs：
3.1.1 single dose toxicity test
3.1.2 screening extensively：EC50 values according to single dose, devise 13 proportionings, each 2 dosage of proportioning, with when
Dose design such as table 1.
The extensive screening test dose design of table 1
3.1.3 Toxicity baseline screening：
According to the result of extensive screening, design albendazole is matched with the compounding of 2-cyano-3-amino-3-phenylancryic acetate, totally 1: 5,1: 7.5,1: 10,
1: 12.5,1: 15 grade 5 assembles ratio.
Test dose design is shown in Table 2
The test dose of table 2 is designed
It is prepared by 3.2 pastille culture mediums
Treatment agent acetone solution, is configured to mother liquor.By test medicament and comparison medicament according to certain concentration gradient,
It is separately added into sterilized and is cooled to mixing in 45 DEG C or so of culture medium, pour into plate, is made pastille flat board.
3.3 toxicity tests
Using mycelial growth rate method.A bacterium dish is connect in pastille flat board center, mycelia is down.25 °C are cultivated to blank
When bacterium colony is covered with culture dish more than 2/3, the colony diameter of each treatment is measured.Each bacterium colony is measured 2 times by crossing method, with it
Average represents the size of bacterium colony.Calculate suppression of the medicament to thalli growth rate.
3.4 evaluation methods
3.4.1 synergistic effect (Me-Mt) method
Me-Mt=Me- [P1+P2(l-P1)]
Me-Mt values are synergistic effect more than 0, are antagonism less than 0.(P1 and P2It is the actual preventive effect of each single dose, Me is
Actual preventive effect, Mt is theoretical preventive effect.)
3.4.2 co-toxicity coefficient method
Content (the %)+B of the toxicity index × A of theoretical toxicity index TTI=A in mixture
Contents (%) of the toxicity index × B in mixture
Co-toxicity coefficient is more than 120, shows there is synergistic effect；Show there is antagonism less than 80；Show have between 80~120
Summation action.
4 result of the tests
The toxicity test of 4.1 single doses
The toxicity test result of the albendazole of table 3 and 2-cyano-3-amino-3-phenylancryic acetate to wheat scab
The poisoning of albendazole and 2-cyano-3-amino-3-phenylancryic acetate to fusarium graminearum has excellent activity, EC50Value is respectively 0.3646 μ
G/ml and 0.2304 μ g/ml, EC90Value is respectively 2.4232 μ g/ml and 1.9014 μ g/ml.The activity of 2-cyano-3-amino-3-phenylancryic acetate is better than rosickyite
Imidazoles.
4.2 extensive the selection results
The extensive the selection result of table 4
As seen from Table 4, dosage 1 is consistent with the synergistic effect trend of dosage 2.Proportioning more than 0 is 1: 15,1: 10,
With 1: 8, in proportioning of the synergistic effect more than 0, be peak at 1: 10, designs 1: 5,1 in the experiment of test Toxicity baseline:
7.5th, 1: 10,1: 12.5,1: 15 grade 5 assembles ratio.
4.3 albendazoles and 2-cyano-3-amino-3-phenylancryic acetate 5 are with the toxicity test for comparing wheat scab
The albendazole of table 5 matches somebody with somebody the toxicity test for comparing fusarium graminearum with 5 compoundings of 2-cyano-3-amino-3-phenylancryic acetate
From table 5,5 EC of quality proportioning of albendazole and 2-cyano-3-amino-3-phenylancryic acetate50It is 0.2125~0.2434 μ g/ml to be worth,
EC90It is 1.7528~2.3284 μ g/ml to be worth.Quality proportioning be 1: 7.5 and 1: 10 bacteriostatic activity be higher than albendazole and cyanogen alkene
Two single doses of bacterium ester, other 3 are assembled than bacteriostatic activity between two single doses.
4.4 synergy evaluations
The albendazole of table 6 matches somebody with somebody the synergy evaluation for comparing wheat scab with 5 compoundings of 2-cyano-3-amino-3-phenylancryic acetate
From table 6,5 co-toxicity coefficients of quality proportioning of albendazole and 2-cyano-3-amino-3-phenylancryic acetate are 97.31~108.28, equal table
It is now summation action, the result of co-toxicity coefficient is consistent with extensive screening, wherein 1: 10 co-toxicity coefficient is maximum.
5 conclusions
Albendazole has excellent bacteriostatic activity to fusarium graminearum with 2-cyano-3-amino-3-phenylancryic acetate.
Killing fusarium graminearum after both compoundings has summation action, with reference to EC50、EC90, 3 fingers such as co-toxicity coefficient
Mark, albendazole is 1: 10 with the best in quality proportioning of 2-cyano-3-amino-3-phenylancryic acetate, prevents and treats wheat scab effect is significant.
Compound disinfectant of the invention, two kinds of base-materials are not chemically reacted, and effect is added with obvious, use peace
Entirely, have a good cytotoxicity for the different types of fusarium graminearum in current field, effect apparently higher than albendazole or
2-cyano-3-amino-3-phenylancryic acetate single dose.The built agent can also effectively prevent and treat the crops such as take-all, bakanae disease of rice, tomato, cucumber
The diseases such as base rot disease, root-rot and marrow necrosis.It is used to prevent and treat wheat scab suitable for vast rural area.
Specific embodiment
Embodiment 1：97% albendazole active compound 2.5kg and Jiang Sunan of Guizhou Daoyuan Biotechnology Co., Ltd.'s offer is provided
The 95% 2-cyano-3-amino-3-phenylancryic acetate active compound 12.5kg that square pesticide research center provides is base-material, is re-dubbed 15kg and prevents and treats answering for wheat scab
Close bactericide.
Embodiment 2：97% albendazole active compound 2.0kg and Jiang Sunan of Guizhou Daoyuan Biotechnology Co., Ltd.'s offer is provided
The 95% 2-cyano-3-amino-3-phenylancryic acetate active compound 15.0kg that square pesticide research center provides is base-material, is re-dubbed 17kg and prevents and treats answering for wheat scab
Close bactericide.
Embodiment 3：97% albendazole active compound 1.0kg and Jiang Sunan of Guizhou Daoyuan Biotechnology Co., Ltd.'s offer is provided
The 95% 2-cyano-3-amino-3-phenylancryic acetate active compound 10.0kg that square pesticide research center provides is base-material, is re-dubbed 11kg and prevents and treats answering for wheat scab
Close bactericide.
Embodiment 4：97% albendazole active compound 1.0kg and Jiang Sunan of Guizhou Daoyuan Biotechnology Co., Ltd.'s offer is provided
The 95% 2-cyano-3-amino-3-phenylancryic acetate active compound 12.5kg that square pesticide research center provides is base-material, is re-dubbed 13.5kg and prevents and treats wheat scab
Compound disinfectant.
Embodiment 5：97% albendazole active compound 1.0kg and Jiang Sunan of Guizhou Daoyuan Biotechnology Co., Ltd.'s offer is provided
The 95% 2-cyano-3-amino-3-phenylancryic acetate active compound 15.0kg that square pesticide research center provides is base-material, is re-dubbed 16kg and prevents and treats answering for wheat scab
Close bactericide.
The compound disinfectant is used in Guizhou somewhere, obtains treatment wheat scab remarkable result.
Claims (2)
1. a kind of compound disinfectant for preventing and treating wheat scab, it is characterised in that the compound disinfectant is with rosickyite azoles and cyanogen alkene bacterium
Ester is base-material, according to the compound disinfectant that the quality proportioning 1: 5~1: 15 of albendazole and 2-cyano-3-amino-3-phenylancryic acetate is formulated, described third
Sulphur imidazoles is the active compound of mass fraction 97%, and the 2-cyano-3-amino-3-phenylancryic acetate is 95% active compound.
2. according to the compound disinfectant described in claim 1, it is characterised in that the compound disinfectant is with rosickyite azoles and cyanogen alkene bacterium
Ester is base-material, according to the compound disinfectant that the quality proportioning of the quality proportioning 1: 10 of albendazole and 2-cyano-3-amino-3-phenylancryic acetate is formulated,
The albendazole is the active compound of mass fraction 97%, and the 2-cyano-3-amino-3-phenylancryic acetate is 95% active compound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710119431.3A CN106818803A (en) | 2017-03-02 | 2017-03-02 | The bactericide for preventing and treating wheat scab that rosickyite azoles is compounded with 2-cyano-3-amino-3-phenylancryic acetate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710119431.3A CN106818803A (en) | 2017-03-02 | 2017-03-02 | The bactericide for preventing and treating wheat scab that rosickyite azoles is compounded with 2-cyano-3-amino-3-phenylancryic acetate |
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| Publication Number | Publication Date |
|---|---|
| CN106818803A true CN106818803A (en) | 2017-06-13 |
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| CN201710119431.3A Withdrawn CN106818803A (en) | 2017-03-02 | 2017-03-02 | The bactericide for preventing and treating wheat scab that rosickyite azoles is compounded with 2-cyano-3-amino-3-phenylancryic acetate |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416625A (en) * | 2008-11-28 | 2009-04-29 | 陕西韦尔奇作物保护有限公司 | Agricultural bactericide containing JS399-19 and use thereof |
| CN104106580A (en) * | 2014-06-03 | 2014-10-22 | 贵州道元生物技术有限公司 | Compound bactericide for preventing and treating wheat scab |
-
2017
- 2017-03-02 CN CN201710119431.3A patent/CN106818803A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416625A (en) * | 2008-11-28 | 2009-04-29 | 陕西韦尔奇作物保护有限公司 | Agricultural bactericide containing JS399-19 and use thereof |
| CN104106580A (en) * | 2014-06-03 | 2014-10-22 | 贵州道元生物技术有限公司 | Compound bactericide for preventing and treating wheat scab |
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Application publication date: 20170613 |