CN103300023A - Pesticide composition - Google Patents
Pesticide composition Download PDFInfo
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- CN103300023A CN103300023A CN2013102454381A CN201310245438A CN103300023A CN 103300023 A CN103300023 A CN 103300023A CN 2013102454381 A CN2013102454381 A CN 2013102454381A CN 201310245438 A CN201310245438 A CN 201310245438A CN 103300023 A CN103300023 A CN 103300023A
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- tetramycin
- ningnanmycin
- disease
- pesticide
- composition
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Abstract
The invention belongs to the technical field of pesticides and relates to a pesticide composition. The pesticide composition is characterized by being prepared from active ingredients including tetramycin and ningnanmycin, wherein the weight ratio of the two active ingredients is 1:150-150:1. The composition can be prepared to a water agent and is used for preventing a rice sheath blight disease; and the composition is remarkable in sterilization effect and is environment-friendly.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to contain the composition pesticide of tetramycin and Ningnanmycin.
Background technology
Tetramycin (Tetramycin) claim again tetramycin, is by a strain that separates in the soil sample of Wuzhou, the Guangxi stimulation metabolite of streptomyces hygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp) not.This product fungicidal spectrum is wide, and three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungi are all had extremely strong killing action.The control of the applicable multiple fungal disease of various crops.Especially to fruit tree putrefaction disease, spot defoliation, cotton yellow (withered) wither disease, root rot, rice sheath blight disease, seedling blight, ginseng and pseudo-ginseng black spot, tealeaves tea gall, fruit white rot of grape, the climing rot of watermelon, root rot, the rotten special efficacy of stem foot.This composition can obviously promote the callus healing simultaneously, promotes flourishing, the aging root system recovery of weak shoot root system, improves the crop disease-resistant ability and optimizes crop quality.
Ningnanmycin (ningnanmycin) is by the tunning that is isolated fungi at Ningnan County, Sichuan Province soil, is a kind of Cystosine nucleocide type antibiotics.This antibiotic is a kind of low toxicity, low-residual, without " three cause " and accumulation problems, novel pesticide free from environmental pollution, obvious to the bacterial blight of rice relative control effect, it is to wheat in addition, vegetables, the diseases prevention of the powdery mildews such as flowers, effect of increasing production is all very remarkable, to small round sclerotial disease of rice, Olive peacock's eye disease, shot hole and tobacco mosaic preventive effect are also fine, simultaneously at tobacco mosaic virus disease, tomato virus disease, pepper virus disease, rice seedling blight, root rot, stripe disease, alternaria leaf spot of apple, obtained registration on the powdery mildew of cucumber, this external control sclerotinia rot of colza, peronophythora litchi, other crop virosis, stem rot, climing rot, also large scale application on the multiple diseases such as powdery mildew.
Rice sheath blight disease claims again moire disease, is to be infected by Rhizoctonia solani Kuhn to fall ill, and how to fall ill under high temperature, super-humid conditions.Banded sclerotial blight is serious in the harm of south rice zone, is one of Major Diseases on the current Rice Production.This disease can make paddy rice not ear, and particle weight descends.
Along with a large amount of uses of agricultural chemicals, pesticide hazards problem and people become increasingly conspicuous to the concern of living environment.For the impact of present agricultural chemicals on environment, people begin more to be conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between the microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of exploitation biogenic pesticide more and more are subject to people's attention at present, and two kinds of active ingredients that relate among the present invention are biogenic pesticide, show by our test in the past, two kinds of mixed pesticides that mechanism of action is different, can not only postpone resistance produces, can also improve drug effect, reduce usage amount, reduce peasant's dispenser cost.Therefore we are composite with tetramycin and Ningnanmycin, in order to enlarge fungicidal spectrum, reduce peasant's labour cost.
Summary of the invention
The purpose of this invention is to provide a kind of composition pesticide with synergistic effect and safety and environmental protection, realize improving drug effect, reduce the purpose of polluting, reducing cost.Be specifically related to the composite composition pesticide of tetramycin and Ningnanmycin.
Utilize composition pesticide of the present invention, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, this formulation is aqua.
In the above-mentioned composition pesticide, the weight ratio of tetramycin and Ningnanmycin is 1:150~150:1.
Above-mentioned composition pesticide has following characteristics: 1. environmentally friendly, two kinds of active ingredients are biogenic, and are very little to environmental influence; 2. bactericidal action is strong, utilizes the antagonism between the microorganism that Oomycete class plant disease is had very strong control efficiency; 3. the adhesive ability at plant surface is strong; 4. two kinds of compositions are without obvious cross resistance, and complex preparation has obvious synergistic effect; 5. delay the generation of active ingredient resistance, reduce peasant's labour cost.
Embodiment
For determining tetramycin and two kinds of composite synergistic effects of active ingredient of Ningnanmycin, the applicant at first uses these two kinds of compositions to carry out the indoor biometrics test, and test method and result are as follows:
After at first having analyzed the effect characteristics of two kinds of active ingredients, the object that we will give birth to survey is decided to be thanatephorus cucumeris(frank) donk (Thanatephorus cucumeris), this Pseudomonas Basidiomycotina fungi, and sclerotium is dark brown, it is light brown to the taupe mildew that bacterium colony is.
The test method of indoor biometrics: adopt growth rate method to measure medicament to the inhibitory action of rice sheath blight disease mycelia growth.On the basis of preliminary experiment, from each medicament the germ mycelial growth inhibition rate is reached 5 concentration of design in 10%~90% scope, first that the former medicine of 95% tetramycin and the former medicine of 70% Ningnanmycin is for subsequent use with the liquid that the sterile water dissolving is made into series concentration, then with liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that to make the band medicine in the culture dish (Φ 90mm) of sterilization dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Card punch (Φ 5mm) picking Rhizoctonia solani Kuhn bacterium cake with sterilization, mycelia faces down aseptic inoculation in the central authorities with the medicine flat board, place 25 ℃ interior cultivation of constant incubator, cultivate and adopt the right-angled intersection method to measure colony diameter after 6 days, calculate each medicament to the EC of pathogen
50, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Used former medicine is tested in above-mentioned living test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
Co-toxicity coefficient CTC was synergistic effect greater than 120 o'clock, less than 80 o'clock be antagonism, be addition in the time of between 80~120.
The indoor biometrics test data sees the following form:
Table 1 tetramycin, Ningnanmycin and different proportioning are to the measurement result of rice sheath blight disease co-toxicity coefficient
Can be found out by above-mentioned data, when tetramycin and Ningnanmycin are composite in varing proportions, the co-toxicity coefficient (CTC) of prescription is all greater than 120, show that complex preparation has obvious synergistic effect to the preventive effect of rice sheath blight disease, wherein the synergy between 1:5~5:1 of tetramycin and Ningnanmycin is the most remarkable, and visible tetramycin and Ningnanmycin complex preparation can become the desirable medicament of preventing and treating rice sheath blight disease.
Determining by indoor biometrics test that tetramycin and two kinds of active ingredients of Ningnanmycin are composite has a good synergistic effect, below by instantiation in order to further describing the present invention, but the present invention absolutely not only only limits to following these embodiment.Percentage all is weight percentage in the prescription, and employed former medicine and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 15.1% tetramycin Ningnanmycin aqua
Example two, 30.2% tetramycin Ningnanmycin aqua
Example three, 20.2% tetramycin Ningnanmycin aqua
Example four, 10.1% tetramycin Ningnanmycin aqua
Example five, 20.4% tetramycin Ningnanmycin aqua
Example six, 25.5% tetramycin Ningnanmycin aqua
Example seven, 11% tetramycin Ningnanmycin aqua
Example eight, 6.6% tetramycin Ningnanmycin aqua
Example nine, 8% tetramycin Ningnanmycin aqua
Select above example to prevent and treat the field trial of rice sheath blight disease, the prescription determined is prevented and treated the effect of rice sheath blight disease, simultaneously by with the Contrast on effect of 5% tetramycin AS, 5% Ningnanmycin AS, verify the synergistic effect after composite.
8% tetramycin Ningnanmycin aqua and other preparation are done the test of pesticide effectiveness of field control rice sheath blight disease.Test site is the Hanchuan City, Hubei Province, and the time is on May 15th, 2012, test each and process three repetitions, and the residential quarter randomized arrangement, each residential quarter area is 100m
2, adopt the back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all residential quarters all planting habit with the locality is identical, records the dispenser same day and the synoptic data in a week thereafter, and is specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each residential quarter diagonal, and every some straight ahead is decided 25 clumps, and fixed point investigation in the 7th day, 21 days is investigated each residential quarter disease and is referred to after the dispenser, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
Table 38% tetramycin Ningnanmycin aqua and different medicament are to the preventive effect of rice sheath blight disease
Annotate: data are the mean value that repeats for 3 times in the table, and letter is all Duncan testing differentia remarkable time mutually: in May, 2012 place; Hanchuan, Hubei
From above-mentioned data, can find out, tetramycin and Ningnanmycin complex preparation behind medicine 7 days preventive effects all above 70%, and 5% tetramycin AS, 7 days preventive effects behind medicine are 40.85%, the preventive effect of 5% Ningnanmycin AS is 38.73%, compare tetramycin and Ningnanmycin single dose, the preventive effect of complex preparation and single dose all have extremely significant difference, after visible two kinds of active ingredients are composite good synergistic effect are arranged.And behind medicine 21 days, all complex preparations have all surpassed 84% to the preventive effect of rice sheath blight disease, show that complex preparation has not only prolonged the lasting period, and synergistic effect is remarkable, have improved economic benefit.
Claims (3)
1. composition pesticide, it is characterized in that: described active component is comprised of tetramycin and Ningnanmycin, and the weight ratio of tetramycin and Ningnanmycin is 1:150~150:1.
2. composition pesticide according to claim 1 is characterized in that: make aqua by active ingredient and auxiliary material.
3. it is characterized in that according to claim 1 or composition pesticide claimed in claim 2: be used for preventing and treating rice sheath blight disease.
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CN2013102454381A CN103300023A (en) | 2013-06-19 | 2013-06-19 | Pesticide composition |
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CN2013102454381A CN103300023A (en) | 2013-06-19 | 2013-06-19 | Pesticide composition |
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CN2013102454381A Pending CN103300023A (en) | 2013-06-19 | 2013-06-19 | Pesticide composition |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103535370A (en) * | 2013-10-15 | 2014-01-29 | 梅秀芳 | Pesticide composition |
CN103598192A (en) * | 2013-11-30 | 2014-02-26 | 海利尔药业集团股份有限公司 | Bactericidal composition containing tetramycin and zhongshengmycin |
CN103609572A (en) * | 2013-11-30 | 2014-03-05 | 海利尔药业集团股份有限公司 | Sterilization composition containing tetramycin and amino-oligosaccharin |
CN103636622A (en) * | 2013-12-02 | 2014-03-19 | 海利尔药业集团股份有限公司 | Sterilizing composition containing tetramycin and bromothalonil |
CN106912490A (en) * | 2017-03-10 | 2017-07-04 | 陕西康禾立丰生物科技药业有限公司 | A kind of efficient bactericidal composite containing tetramycin |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101633941A (en) * | 2008-07-24 | 2010-01-27 | 辽宁省微生物科学研究院 | Method for preparing biopesticide tetramycin and application |
CN102293205A (en) * | 2011-05-27 | 2011-12-28 | 江苏省绿盾植保农药实验有限公司 | Osthole and ningnanmycin compound bactericide |
-
2013
- 2013-06-19 CN CN2013102454381A patent/CN103300023A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101633941A (en) * | 2008-07-24 | 2010-01-27 | 辽宁省微生物科学研究院 | Method for preparing biopesticide tetramycin and application |
CN102293205A (en) * | 2011-05-27 | 2011-12-28 | 江苏省绿盾植保农药实验有限公司 | Osthole and ningnanmycin compound bactericide |
Non-Patent Citations (1)
Title |
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余露: "生物农药-梧宁霉素", 《农药市场信息》, no. 21, 11 August 2011 (2011-08-11), pages 33 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103535370A (en) * | 2013-10-15 | 2014-01-29 | 梅秀芳 | Pesticide composition |
CN103598192A (en) * | 2013-11-30 | 2014-02-26 | 海利尔药业集团股份有限公司 | Bactericidal composition containing tetramycin and zhongshengmycin |
CN103609572A (en) * | 2013-11-30 | 2014-03-05 | 海利尔药业集团股份有限公司 | Sterilization composition containing tetramycin and amino-oligosaccharin |
CN103598192B (en) * | 2013-11-30 | 2016-01-06 | 海利尔药业集团股份有限公司 | A kind of bactericidal composition containing tetramycin and Zhongshengmycin |
CN103609572B (en) * | 2013-11-30 | 2016-01-20 | 海利尔药业集团股份有限公司 | A kind of bactericidal composition containing tetramycin and amino-oligosaccharide |
CN103636622A (en) * | 2013-12-02 | 2014-03-19 | 海利尔药业集团股份有限公司 | Sterilizing composition containing tetramycin and bromothalonil |
CN106912490A (en) * | 2017-03-10 | 2017-07-04 | 陕西康禾立丰生物科技药业有限公司 | A kind of efficient bactericidal composite containing tetramycin |
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Address after: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant after: Beijing Yanhua Yongle Biotechnology Co., Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant before: Beijing Yoloo Pesticide Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: BEIJING YOLOO PESTICIDE CO., LTD. TO: BEIJING YANHUA YONGLE BIOTECHNOLOGYCO., LTD. |
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Application publication date: 20130918 |