CN104054727A - Sterilization composition containing phenazino-1-carboxylic acid - Google Patents

Abstract

The invention relates to a sterilization composition containing phenazino-1-carboxylic acid. The sterilization composition comprises two active components, namely phenazino-1-carboxylic acid and polyoxins, wherein the weight ratio of the two active components is 1 to 100-100 to 1. The composition is used for preventing and treating rice sheath blight diseases, achieves an outstanding sterilization effect and is safe to crops.

Description

Bactericidal composition containing Shen piperazine mycin

Technical field the invention belongs to technical field of pesticide, contains specifically the bactericidal composition with synergistic effect of Shen piperazine mycin and polyoxin.

Background technology

Shen piperazine mycin (phenazino-1-carboxylic acid) is a kind of antibiotic through biological culture secretion by pseudomonas fluorescens, the dual-use function simultaneously with wide spectrum Suppressing phytopathogens Promoting plant growth effect, this biological bactericide has wide spectrum, efficient feature, can effectively prevent and treat paddy rice, wheat, fusarium wilt on the crops such as vegetables, climing rot, epidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and soil-borne disease soil treatment.

Polyoxin (polyoxin) is the metabolite that streptomyces aureus produces, and belongs to broad-spectrum antibiotics series bactericidal agent.In having preferably, inhale conduction.Its mechanism of action is to disturb the chitinous biosynthesis of germ cell wall, makes somatic cells wall can not carry out biosynthesis and causes germ dead.After germ tube contacts medicament with mycelia, cell inclusion is expanded, breaks, overflowed in part, and can not normal development, causes death.Therefore also there is the effect that germ produces spore and scab expansion that suppresses.Because polyoxin is a kind of efficient, low toxicity, the safety pesticide of non-environmental-pollution, so be widely used in cereal crops, special crop, the control of the important disease such as fruits and vegetables.

Rice sheath blight disease claims again moire disease, is to be infected and fallen ill by Rhizoctonia solani Kuhn, how under high temperature, super-humid conditions, to fall ill.Banded sclerotial blight is serious in the harm of south rice region, is one of Major Diseases in current Rice Production.This disease can make paddy rice not ear, and particle weight declines.

Along with a large amount of uses of agricultural chemicals, pesticide hazards problem and people become increasingly conspicuous to the concern of living environment.Impact for current agricultural chemicals on environment, people start to be more conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of exploitation biogenic pesticide are more and more subject to people's attention at present, and two kinds of active ingredients that relate in the present invention are biogenic pesticide, by our experiment in the past, show, two kinds of mixed pesticides that mechanism of action is different, can not only postpone resistance produces, can also improve drug effect and residual period, reduce usage amount, reduce peasant's dispenser cost.Therefore we are composite by Shen piperazine mycin and polyoxin, in order to expand fungicidal spectrum, reduce peasant's labour cost.

Summary of the invention

The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the bactericidal composition of Shen piperazine mycin and polyoxin.

Utilize bactericidal composition of the present invention, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make formulation of the present invention, comprise water dispersible granules and suspending agent.

In above-mentioned bactericidal composition, the weight ratio of Shen piperazine mycin and polyoxin is 1:100-100:1.

Above-mentioned bactericidal composition has following characteristics: 1. active ingredient is biogenic, safety and environmental protection; 2. utilize the antagonism between microorganism to carry out sterilization, ability to function is strong; 3. formulation environmental protection, does not add organic principle, convenient transportation and use; 4. two kinds of active ingredients are composite, also have synergistic effect; 5. twenty percent divide between without cross resistance; 6. delay the generation of active ingredient resistance, reduce peasant's labour cost.

Embodiment

For determining Shen piperazine mycin and two kinds of composite synergistic effects of active ingredient of polyoxin, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:

First analyzed after the effect feature of two kinds of active ingredients, we are decided to be thanatephorus cucumeris(frank) donk (Thanatephoruscucumeris) by the raw object of surveying, this Pseudomonas Basidiomycotina fungi, and sclerotium is dark brown, it is light brown to taupe mildew that bacterium colony is, and this bacterium can cause rice sheath blight disease.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of rice sheath blight disease mycelia.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the 95% former medicine of Shen piperazine mycin and the former medicine of 70% polyoxin be take methyl alcohol as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Card punch (Φ 5mm) picking Rhizoctonia solani Kuhn bacterium cake with sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen ₅₀, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).

It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.

Indoor biometrics test data sees the following form:

Table 1 Shen piperazine mycin, polyoxin and the measurement result of different proportioning to rice sheath blight disease co-toxicity coefficient thereof

Analyze above-mentioned data, can obviously find out when Shen piperazine mycin and polyoxin are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 125, the control of rice sheath blight disease is had to the synergistic effect of different proportion, visible, the composite desirable medicament of preventing and treating rice sheath blight disease that becomes of Shen piperazine mycin and polyoxin.

By indoor biometrics, test, checking Shen piperazine mycin and two kinds of active ingredients of polyoxin are composite has a good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 20.2% Shen piperazine mycin polyoxin suspending agent

Example two, 25.5% Shen piperazine mycin polyoxin water dispersible granules

Example three, 18.6% Shen piperazine mycin polyoxin suspending agent

Example four, 18% Shen piperazine mycin polyoxin water dispersible granules

Example five, 10% Shen piperazine mycin polyoxin suspending agent

Example six, 50.5% Shen piperazine mycin polyoxin water dispersible granules

Example seven, 31% Shen piperazine mycin polyoxin suspending agent

Example eight, 55% Shen piperazine mycin polyoxin water dispersible granules

Example nine, 24% Shen piperazine mycin polyoxin suspending agent

Select above example to prevent and treat the field trial of rice sheath blight disease, the formula of determining is prevented and treated the effect of rice sheath blight disease; Simultaneously by the Contrast on effect with Shen piperazine mycin and polyoxin single dose, verify the synergistic effect after composite.Concrete test method data are as follows:

10% Shen piperazine mycin polyoxin suspending agent and other preparation are prevented and treated the field control effectiveness test of rice sheath blight disease.Test site is Hanchuan City, Hubei Province, and the time is on June 20th, 2013, test each and process three repetitions, and community randomized arrangement, each community area is 100m ², adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.

The grade scale of disease index:

0 grade: without scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for 6%～10% of whole leaf area;

5 grades: lesion area accounts for 11%～25% of whole leaf area;

7 grades: lesion area accounts for 26%～50% of whole leaf area;

9 grades: lesion area accounts for the more than 51% of whole leaf area.

Table 3 10% Shen piperazine mycin polyoxin suspending agent and the preventive effect of different medicament to rice sheath blight disease

From above-mentioned data, can find out, Shen piperazine mycin and polyoxin complex preparation after medicine 7 days preventive effects all over 75%, and piperazine mycin single dose 7 days preventive effects after medicine in Shen are 55.97%, the preventive effect of polyoxin single dose is 47.36%, by contrast, complex preparation and single dose preventive effect difference are extremely remarkable, after visible two kinds of active ingredients are composite, have good synergistic effect.And after medicine 21 days, all complex preparations have all surpassed 82% to the preventive effect of rice sheath blight disease, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of rice sheath blight disease, alleviates peasant's the amount of labour, has very high practicality.

Claims (2)

1. containing a bactericidal composition for Shen piperazine mycin, it is characterized in that: active component is comprised of Shen piperazine mycin and polyoxin, the weight ratio of described Shen piperazine mycin and polyoxin is 1:100～100:1.

2. bactericidal composition according to claim 1, is characterized in that: by active ingredient and auxiliary material, make suspending agent, water dispersible granules.