CN103271050A - Compound bactericide - Google Patents
Compound bactericide Download PDFInfo
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- CN103271050A CN103271050A CN2013102449720A CN201310244972A CN103271050A CN 103271050 A CN103271050 A CN 103271050A CN 2013102449720 A CN2013102449720 A CN 2013102449720A CN 201310244972 A CN201310244972 A CN 201310244972A CN 103271050 A CN103271050 A CN 103271050A
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- China
- Prior art keywords
- pyraclostrobin
- bactericide
- piperazine mycin
- shen piperazine
- downy mildew
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The invention belongs to the technical field of pesticides, and relates to a compound bactericide. The bactericide is characterized in that active ingredients comprise pyraclostrobin and phenazino-1-carboxylic acid; and the weight ratio of pyraclostrobin to phenazino-1-carboxylic acid is (1:10) to (30:1). The compound bactericide can be made into a suspending agent and a water dispersible granule, and is used for preventing cucumber downy mildew; the sterilizing effect is obvious; and the bactericide is safe for the plants.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to the composite bactericide of a kind of pyraclostrobin-containing and Shen piperazine mycin.
Background technology
Pyraclostrobin (Pyraclostrobin) is novel low toxicity, efficient, wide-spectrum bactericide.This composition is the mitochondrial respiratory inhibitor. namely passes through in cytochrome are synthetic, to stop electronics to shift, thereby reaches the purpose of sterilization.This composition has protection, treatment, blade infiltration conduction.Pyraclostrobin has the better prevention effect to powdery mildew of cucumber, downy mildew and banana freckle, leaf spot.Experiment shows cucumber, banana safety this composition through field efficacy, does not see that poisoning takes place.
Shen piperazine mycin (phenazino-1-carboxylic acid) is by a kind of antibiotic of pseudomonas fluorescens through the biological culture secretion, has the dual-use function that wide spectrum suppresses phytopathogen and promotes plant growth effect simultaneously, this biological bactericide has wide spectrum, characteristics of high efficiency can effectively be prevented and treated paddy rice, wheat, fusarium wilt on the crops such as vegetables, climing rot, eqpidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and soil-borne disease soil treatment.
Cucumber downy mildew is relatively severe diseases of cucumber, becomes seedling stage strain all can be injured, mainly cause harm blade and stem, and tendril and bennet are injured less.Cucumber downy mildew optimum onset temperature is 16~24 ℃, the morbidity of seedling phase, the irregular chlorisis yellowish-brown spot of the positive generation of cotyledon becomes the strain morbidity, how from the forward position, greenhouse, water stain shape, light green fleck appear in morbidity strain middle and lower part blade reverse side before this, and the front does not show, and back scab enlarges gradually, front exposed, scab flavescence brown is limited by vein, and scab is polygonal.Downy mildew is modal a kind of disease on the cucumber, and seedling stage, one-tenth strain phase all can catch an illness.Usually begin morbidity by plant middle part blade earlier, the chlorisis spot appears in portion's expansion up and down on the their early stage blade gradually, and the expansion back forms the irregular shape scab of yellowish-brown.Vacuum side of blade was water soaking mode earlier when humidity was high, then produced the mould layer of grey black, and in the later stage, scab and near mesophyll are rust.Blade is covered with scab when seriously ill, and mutually in flakes, it is withered to cause leaf margin to curl, and last blade is withered and yellow.This disease has bigger influence to the output of cucumber, is badly in need of special efficacy, long medicament of lasting period to cucumber downy mildew on the market.
The random use of agricultural chemicals at present makes pesticide hazards problem and people become increasingly conspicuous to the concern of living environment.The cost of a novel germicide composition of exploitation is quite big, and it is then much smaller relatively to have the composite cost of developing of composition now.Show that by our former experiments two kinds of mixed pesticides that mechanism of action is different can not only be postponed resistance and be produced, can also improve drug effect and prolong the lasting period, reduce usage amount, reduce peasant's dispenser cost.Therefore we are composite with pyraclostrobin and Shen piperazine mycin, in order to enlarge fungicidal spectrum, reduce peasant's labour cost.
Summary of the invention
The purpose of this invention is to provide a kind of composite bactericide with synergistic effect and safety and environmental protection, realize improving drug effect, reduce the purpose of polluting, reducing cost.Be specifically related to the composite bactericide of pyraclostrobin and Shen piperazine mycin.
Utilize composite bactericide of the present invention, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with those skilled in that art's known method, comprise suspending agent and water dispersible granules.
In the above-mentioned composite bactericide, the weight ratio of pyraclostrobin and Shen piperazine mycin is 1:10~30:1.
Described composite bactericide has following characteristics: 1. two kinds of active ingredients are low toxicity, and are very little to environmental influence; 2. formulation advanced person, organic solvent-free adds; 3. two kinds of compositions do not have tangible cross resistance; 4. two kinds of active components are composite, have obvious synergistic effect; 5. delay the generation of active ingredient resistance, reduce peasant's labour cost.
Embodiment
Be checking pyraclostrobin and two kinds of composite synergistic effects of active ingredient of Shen piperazine mycin, the applicant at first uses these two kinds of compositions to carry out the indoor biometrics test, and test method and result are as follows:
After at first having analyzed the effect characteristics of two kinds of active ingredients, the object that we will give birth to survey is decided to be Cuba artificial downy mildew (Pseudoperonospora cubensis), and this Pseudomonas Mastigomycotina fungi is the being bacterial parasite, only can be parasitic on the life plant, the Differentiation of Pathogenicity phenomenon is arranged.The sporangium of this germ is propagated by air-flow and rainwater, can cause the downy mildew of cucumber.
The test method of indoor biometrics: adopt growth rate method to measure medicament to the inhibitory action of cucumber downy mildew mycelia growth.On the basis of preliminary experiment, from each medicament the germ mycelial growth inhibition rate is reached 5 concentration of design in 10%~90% scope, it is standby earlier with the former medicine of 95% pyraclostrobin and the 95% Shen former medicine of piperazine mycin with methyl alcohol to be that solvent is made into the soup of series concentration, then with soup in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour into and make band medicine flat board in the culture dish (Φ 90mm) of sterilization, every processing repeats for four times, and the methyl alcohol that does not have soup with adding equivalent is treated to blank; Card punch (Φ 5mm) picking bacterium of downy mildew of cucumber bacterium cake with sterilization, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, place 25 ℃ interior cultivation of constant incubator, cultivate and adopt the right-angled intersection method to measure colony diameter after 6 days, calculate each medicament to the EC50 of pathogen, and calculate toxicity index, the virulence of more different medicaments and bacteriostasis according to the following equation.Used former medicine is tested in above-mentioned living test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
Co-toxicity coefficient CTC was synergistic effect greater than 120 o'clock, less than 80 o'clock be antagonism, be addition in the time of between 80-120.
Table 1 pyraclostrobin is given birth to the measurement result that test is tested with Shen piperazine mycin and different proportioning to cucumber downy mildew
By above-mentioned data as can be seen, when pyraclostrobin and Shen piperazine mycin were composite in varing proportions, the co-toxicity coefficient of prescription (CTC) had obvious synergistic effect all greater than 140.As seen, the composite desirable medicament that becomes the control cucumber downy mildew of pyraclostrobin and Shen piperazine mycin.
Have a good synergistic effect by indoor biometrics verification experimental verification pyraclostrobin and two kinds of active ingredients of Shen piperazine mycin are composite, below by instantiation in order to further describing the present invention, but the present invention absolutely not only only limits to following these embodiment.Percentage all is weight percentage in the prescription, and employed former medicine and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 11% pyraclostrobin Shen piperazine mycin suspending agent
Example two, 24% pyraclostrobin Shen piperazine mycin suspending agent
Example three, 20% pyraclostrobin Shen piperazine mycin water dispersible granules
Example four, 21% pyraclostrobin Shen piperazine mycin water dispersible granules
Example five, 6% pyraclostrobin Shen piperazine mycin suspending agent
Example six, 15% pyraclostrobin Shen piperazine mycin suspending agent
Example seven, 18% pyraclostrobin Shen piperazine mycin water dispersible granules
Example eight, 22% pyraclostrobin Shen piperazine mycin water dispersible granules
Example nine, 31% pyraclostrobin Shen piperazine mycin water dispersible granules
Select above example to prevent and treat the field trial of cucumber downy mildew, determine the effect of prescription control cucumber downy mildew, simultaneously by with the effect contrast of 5% pyraclostrobin SC, 5% Shen piperazine mycin SC, verify the synergistic effect after composite.
6% pyraclostrobin Shen piperazine mycin SC and other preparation are done the test of pesticide effectiveness of field control cucumber downy mildew.Test site is the Xiaogan Prefecture, Hubei Province, and the time is on June 10th, 2012, test each and handle three repetitions, and the residential quarter randomized arrangement, each residential quarter area is 90m
2, adopt the back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all residential quarters all planting habit with the locality is identical, records the dispenser same day and the synoptic data in a week thereafter, and is specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each residential quarter diagonal, and every some straight ahead is decided 25 clumps, and fixed point investigation in the 7th day, 21 days is investigated each residential quarter disease and is referred to after the dispenser, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%~10% of whole leaf area;
5 grades: lesion area accounts for 11%~25% of whole leaf area;
7 grades: lesion area accounts for 26%~50% of whole leaf area;
9 grades: lesion area accounts for more than 51% of whole leaf area.
Table 3 6% pyraclostrobin Shen piperazine mycin suspending agent and different medicament are to the preventive effect of cucumber downy mildew
Annotate: data are the mean value that repeats for 3 times in the table, and letter is all Duncan testing differentia remarkable time mutually: in June, 2012 place; The Hubei Xiaogan
From above-mentioned data as can be seen, pyraclostrobin and Shen piperazine mycin complex preparation behind medicine 7 days preventive effects all above 75%, the preventive effect of example five is the highest, reach 81.39%, and 5% pyraclostrobin SC, 7 days preventive effects behind medicine are 43.51%, 5% Shen piperazine mycin SC, preventive effect of 7 days behind medicine is 40.48%, and it is extremely remarkable that difference is compared in the preventive effect of complex preparation and single agent, and visible two kinds of active ingredients are composite good synergistic effect.And behind medicine 21 days, complex preparation has all surpassed 84% to the preventive effect of cucumber downy mildew, compares with 7 days preventive effect behind the medicine, and raising is in various degree all arranged, obviously, complex preparation is efficient, low toxicity, has prolonged the lasting period, enlarge fungicidal spectrum, reduced labour cost, have certain economic benefits.
Claims (3)
1. composite bactericide, it is characterized in that: described active component is made up of pyraclostrobin and Shen piperazine mycin, and the weight ratio of pyraclostrobin and Shen piperazine mycin is 1:10~30:1.
2. composite bactericide according to claim 1 is characterized in that: make suspending agent, water dispersible granules by active ingredient and auxiliary material.
3. according to claim 1 or the described composite bactericide of claim 2, it is characterized in that: be used for the control cucumber downy mildew.
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CN2013102449720A CN103271050A (en) | 2013-06-19 | 2013-06-19 | Compound bactericide |
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CN2013102449720A CN103271050A (en) | 2013-06-19 | 2013-06-19 | Compound bactericide |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104488950A (en) * | 2014-12-31 | 2015-04-08 | 安徽丰乐农化有限责任公司 | Pyraclostrobin, phenazine-1-carboxylic acid and thiamethoxam containing suspension concentrate for seed coating as well as preparation method and application thereof |
CN107517979A (en) * | 2016-11-22 | 2017-12-29 | 上海农乐生物制品股份有限公司 | A kind of microbicide compositions containing shenqinmycin and 2-cyano-3-amino-3-phenylancryic acetate |
CN107691456A (en) * | 2017-09-15 | 2018-02-16 | 安徽古尔特科技有限公司 | A kind of bactericidal composition containing fluoxastrobin and shenqinmycin |
CN108184862A (en) * | 2017-12-08 | 2018-06-22 | 甄金兰 | A kind of Synergistic bactericidal composition |
CN108477175A (en) * | 2018-05-15 | 2018-09-04 | 张月婵 | Bactericidal composition containing shenqinmycin and triforine |
CN109090128A (en) * | 2018-09-29 | 2018-12-28 | 武汉友芹种苗技术有限公司 | A kind of bactericidal composition for preventing and treating downy mildew of lettuce |
CN111937895A (en) * | 2020-08-19 | 2020-11-17 | 扬州大学 | Application of pyraclostrobin and composition containing pyraclostrobin in prevention and treatment of peach branch blight |
Citations (4)
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US4568675A (en) * | 1983-12-19 | 1986-02-04 | E. R. Squibb & Sons, Inc. | Phenazine ACE inhibitor produced from streptomyces species |
CN1802927A (en) * | 2006-01-19 | 2006-07-19 | 上海交通大学 | Method for preparing bactericidal agent using antagonizing bacteria M18 strain |
CN101705265A (en) * | 2009-11-12 | 2010-05-12 | 上海交通大学 | Method for producing phenazine-1-carboxylic acid by using engineering bacterial strain M18G to carry plasmid pME6032Phz |
CN103155925A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Sterilization composite containing phenazino-1-carboxylic acid |
-
2013
- 2013-06-19 CN CN2013102449720A patent/CN103271050A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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US4568675A (en) * | 1983-12-19 | 1986-02-04 | E. R. Squibb & Sons, Inc. | Phenazine ACE inhibitor produced from streptomyces species |
CN1802927A (en) * | 2006-01-19 | 2006-07-19 | 上海交通大学 | Method for preparing bactericidal agent using antagonizing bacteria M18 strain |
CN101705265A (en) * | 2009-11-12 | 2010-05-12 | 上海交通大学 | Method for producing phenazine-1-carboxylic acid by using engineering bacterial strain M18G to carry plasmid pME6032Phz |
CN103155925A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Sterilization composite containing phenazino-1-carboxylic acid |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104488950A (en) * | 2014-12-31 | 2015-04-08 | 安徽丰乐农化有限责任公司 | Pyraclostrobin, phenazine-1-carboxylic acid and thiamethoxam containing suspension concentrate for seed coating as well as preparation method and application thereof |
CN107517979A (en) * | 2016-11-22 | 2017-12-29 | 上海农乐生物制品股份有限公司 | A kind of microbicide compositions containing shenqinmycin and 2-cyano-3-amino-3-phenylancryic acetate |
CN107691456A (en) * | 2017-09-15 | 2018-02-16 | 安徽古尔特科技有限公司 | A kind of bactericidal composition containing fluoxastrobin and shenqinmycin |
CN107691456B (en) * | 2017-09-15 | 2020-10-02 | 陕西大成作物保护有限公司 | Bactericidal composition containing fluoxastrobin and shenqinmycin |
CN108184862A (en) * | 2017-12-08 | 2018-06-22 | 甄金兰 | A kind of Synergistic bactericidal composition |
CN108477175A (en) * | 2018-05-15 | 2018-09-04 | 张月婵 | Bactericidal composition containing shenqinmycin and triforine |
CN109090128A (en) * | 2018-09-29 | 2018-12-28 | 武汉友芹种苗技术有限公司 | A kind of bactericidal composition for preventing and treating downy mildew of lettuce |
CN111937895A (en) * | 2020-08-19 | 2020-11-17 | 扬州大学 | Application of pyraclostrobin and composition containing pyraclostrobin in prevention and treatment of peach branch blight |
CN111937895B (en) * | 2020-08-19 | 2021-08-13 | 扬州大学 | Application of pyraclostrobin and composition containing pyraclostrobin in prevention and treatment of peach branch blight |
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Address after: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant after: Beijing Yanhua Yongle Biotechnology Co., Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant before: Beijing Yoloo Pesticide Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: BEIJING YOLOO PESTICIDE CO., LTD. TO: BEIJING YANHUA YONGLE BIOTECHNOLOGYCO., LTD. |
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Application publication date: 20130904 |