CN104396978A

CN104396978A - Compound bactericide

Info

Publication number: CN104396978A
Application number: CN201410710655.8A
Authority: CN
Inventors: 杨会营; 刘晓娇; 吴勇; 蔡朝阳; 王�华
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Priority date: 2014-11-28
Filing date: 2014-11-28
Publication date: 2015-03-11

Abstract

The invention belongs to the technical field of pesticides and relates to a compound bactericide. The compound bactericide is characterized in that active components include chlorophenyl ether amide and propamocarb. The compound can be prepared into a suspension agent or water dispersible granules, and has the characteristics that the synergistic effect is remarkable, the drug resistance is delayed while the dosage is reduced, the lasting period is prolonged, and the environment-friendly effect is achieved.

Description

A kind of composite bactericide

Technical field

The invention belongs to technical field of pesticide, contain the composite bactericide of chlorobenzene ether acid amides and Propamocarb specifically.

Background technology

Chlorobenzene ether acid amides is Central China Normal University's pyrazol acid amide compounds newly developed, has good control efficiency, have good preventive effect simultaneously to the banded sclerotial blight of paddy rice and wheat to the disease such as epidemic disease, downy mildew, tikka of the plants such as cucumber, tomato, potato.Field trial shows do not have cross resistance with existing bactericide.Structural formula is as follows:

Propamocarb (Propamocarb) is a kind of low toxicity bactericide with local systemic action, belongs to carbamates.Special efficacy is had to Oomycete fungal.Its bactericidal mechanism mainly suppresses the phosphatide of germ cell membrane component and the biosynthesis of fatty acid, and then suppresses mycelial growth, sporangial formation and sprouting.This bactericidal mechanism is different from other types bactericide, without mutual pesticide resistance.Uptake and translocation is good, when being used as soil treatment, also can being upwards transported to whole plant very soon, when being used as cauline leaf process, can being absorbed by blade very soon and be distributed in blade, play a protective role by root absorption.

Cucumber downy mildew is the more serious disease of cucumber, all can be injured from seedling stage to strain, mainly cause harm blade and stem.This sick optimum onset temperature is 16-24 DEG C, Seedling Stage is fallen ill, there is irregular chlorisis yellowish-brown spot in cotyledon front, strain is fallen ill, many from forward position, greenhouse, there is water stain shape, light green fleck in morbidity strain before this middle and lower part blade reverse side, front does not show, rear scab expands gradually, front exposed.Be badly in need of good to the quick-acting of cucumber downy mildew, that the lasting period is long medicament in the market.

Summary of the invention

The object of this invention is to provide and a kind ofly there is synergistic effect and the composite bactericide of safety and environmental protection, for preventing and treating cucumber downy mildew, realizing improving drug effect, decreasing pollution, the object that reduces costs.Be specifically related to the composite bactericide of chlorobenzene ether acid amides and Propamocarb.

Utilize above-mentioned composite bactericide, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise suspending agent and water dispersible granules.

In above-mentioned composite bactericide, the weight ratio of chlorobenzene ether acid amides and Propamocarb is 1:60-60:1.

Above-mentioned composite bactericide has following characteristics: 1, two kinds of active ingredients composite after, there is very strong synergistic effect; 2, the active ingredient mode of action is novel, with existing bactericide without cross resistance; 3, formulation environmental protection, can be good at playing drug effect; 4, quick-acting is good, and the lasting period is long.

Embodiment

For determine chlorobenzene ether acid amides and Propamocarb two kinds of active ingredients composite after synergistic effect, first applicant uses these two kinds of compositions to carry out indoor biometrics experiment, after the action character analyzing two kinds of active ingredients, the raw object surveyed is decided to be Cuba artificial downy mildew (Pseudoperonospora cubensis) by us, this Pseudomonas Mastigomycotina fungi, can cause the downy mildew of cucumber.

The test method of indoor biometrics: reference standard NY/T1156.7-2006, adopts growth rate method to measure medicament to the inhibitory action of cucumber downy mildew pathogen mycelial growth.On the basis of preliminary experiment, from each medicament, design 5 concentration within the scope of 10%-90% are reached to germ mycelial growth inhibition rate, it is for subsequent use by the 95% former medicine of chlorobenzene ether acid amides and the former medicine of 98% Propamocarb with acetone to be first that solvent is made into the liquid of series concentration, then liquid is joined in 1% ratio in the PDA medium melting also cool to room temperature, after fully shaking up, pour in the culture dish (Φ 90mm) of sterilizing and make band medicine flat board, often process and repeat, to add the process of sterile water for blank for four times; With card punch (Φ 5mm) the picking bacterium of downy mildew of cucumber bacterium cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in the interior cultivation of constant incubator of 25 DEG C, adopt right-angled intersection method to measure colony diameter after cultivating 6d, calculate the EC of each medicament to pathogen ₅₀, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:

The theoretical toxicity index TTI=∑ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).

Be synergistic effect when CTC is greater than 120, being antagonism when being less than 80, is addition time between 80-120.

Table 1 chlorobenzene ether acid amides, Propamocarb and different ratio thereof are to the measurement result of cucumber downy mildew co-toxicity coefficient

Analyze above-mentioned data can find out, when chlorobenzene ether acid amides and Propamocarb are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of cucumber downy mildew is had to the synergistic effect of different proportion.

Tested by indoor biometrics, determine chlorobenzene ether acid amides and Propamocarb two kinds of active ingredients are composite can good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 42.7% chlorobenzene ether acid amides Propamocarb suspending agent

Example two, 48.8% chlorobenzene ether acid amides Propamocarb water dispersible granules

Example three, 13% chlorobenzene ether acid amides Propamocarb suspending agent

Example four, 15.6% chlorobenzene ether acid amides Propamocarb water dispersible granules

Example five, 11% chlorobenzene ether acid amides Propamocarb water dispersible granules

Example six, 22% chlorobenzene ether acid amides Propamocarb water dispersible granules

Example seven, 24% chlorobenzene ether acid amides Propamocarb water dispersible granules

Example eight, 18% chlorobenzene ether acid amides Propamocarb suspending agent

Example nine, 12% chlorobenzene ether acid amides Propamocarb water dispersible granules

Above example is selected to carry out preventing and treating the field trial of cucumber downy mildew, the effect of control cucumber downy mildew of determining to fill a prescription, simultaneously with the Contrast on effect of chlorobenzene ether acid amides and Propamocarb single dose, the control efficiency of the checking embodiment of the present invention.Concrete test method is with reference to GB/T17980.26-2000.Test site is Laiyang Shandong Province area, and the time is on May 1st, 2014, and test each process four repetition, community randomized arrangement, each plot area is 20m ², adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, records the synoptic data on the dispenser same day and a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: every community gets 4 investigation at random, and look into two strains at often, whole blade is investigated in every strain.

The grade scale of disease index:

0 grade: without scab;

1 grade: lesion area accounts for less than 5% of whole leaf area;

3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;

5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;

7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;

9 grades: lesion area accounts for more than 51% of whole leaf area.

Table 3 12% chlorobenzene ether acid amides Propamocarb water dispersible granules and different agents are to the preventive effect of cucumber downy mildew

As can be seen from above-mentioned data, chlorobenzene ether acid amides and Propamocarb complex preparation after medicine 7 days preventive effects all more than 70%, and 24% chlorobenzene ether amide suspending agents, 7 days preventive effects after medicine are 38.84%, the preventive effect of 66.5% Propamocarb aqua is 36.48%, and compare two kinds of single doses, complex preparation preventive effect is extremely remarkable, after medicine 21 days, all complex preparations have all exceeded 72% to the preventive effect of cucumber downy mildew, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of cucumber downy mildew.Visible, complex preparation can well improve drug effect, extends the lasting period, alleviates the amount of labour of peasant, have very high practicality.

Claims

1. a composite bactericide, is characterized in that: active component is made up of chlorobenzene ether acid amides and Propamocarb, and the weight ratio of described chlorobenzene ether acid amides and Propamocarb is 1:60 ~ 60:1.

2. composite bactericide according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active component and auxiliary material.