CN104430370A

CN104430370A - Fungicidal composition

Info

Publication number: CN104430370A
Application number: CN201410602518.2A
Authority: CN
Inventors: 杨会营; 刘晓娇; 吴勇; 蔡朝阳; 惠浩浩
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Priority date: 2014-10-31
Filing date: 2014-10-31
Publication date: 2015-03-25

Abstract

The invention belongs to the technical field of pesticides, and relates to a fungicidal composition. The fungicidal composition is characterized in that active ingredients comprise picoxystrobin and fluorophenylate amide. The composition can be prepared into a suspending agent and a water dispersible granule, the synergism is obvious, and the composition is environmentally friendly.

Description

A kind of bactericidal composition

Technical field

The invention belongs to technical field of pesticide, contain the bactericidal composition with synergistic effect of ZEN 90160 and fluorobenzene ether acid amides specifically.

Background technology

ZEN 90160 (Picoxystrobin) is systemic fungicide, controlling object wide spectrum, is mainly used in the foliage disease of control wheat class and paddy rice as banded sclerotial blight, leaf rust, glume blight, brown spot, powdery mildew etc.This active ingredient belongs to mitochondrial respiratory inhibitor, and due to systemic activity and the fumigation activity of ZEN 90160, thus after dispenser, active ingredient can effectively be reallocated and fully transmit, and compares other compositions, and ZEN 90160 has better result for the treatment of.

Fluorobenzene ether acid amides is Central China Normal University's pyrazol acid amide compounds newly developed, has good preventive effect to the banded sclerotial blight of paddy rice and wheat, also has certain control efficiency to the epidemic disease of the crop such as cucumber, tomato and sigatoka simultaneously.

Rice sheath blight disease, is infected by Rhizoctonia solani Kuhn to fall ill, how falls ill under high temperature, super-humid conditions.Banded sclerotial blight is serious in the harm of south rice region, is one of Major Diseases in current Rice Production.This disease can make paddy rice not ear, and particle weight declines.

Due to the use lack of standardization of current agricultural chemicals, cause disease more and more stronger to the resistance of agricultural chemicals, cause Pesticide use amount more and more higher, cause the pollution of environment.Shown by our experiment and relevant report, combined use of pesticides can be good at the deficiency overcoming existing product, improves drug effect, reduces usage amount.

Summary of the invention

The object of this invention is to provide a kind of bactericidal composition with synergistic effect, for the water prevention rice sheath blight, compared with existing pesticide control, realize the object improving drug effect.Be specifically related to the bactericidal composition of ZEN 90160 and fluorobenzene ether acid amides.

Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, formulation of the present invention is suspending agent and water dispersible granules.

In above-mentioned bactericidal composition, the weight ratio of ZEN 90160 and fluorobenzene ether acid amides is 1:50-50:1.

Above-mentioned bactericidal composition has following characteristics: 1. use complex preparation, has good synergistic effect; 2. the active ingredient mode of action is unique, with existing bactericide without cross resistance; 3. to crop safety, environmentally friendly; 4. bactericidal action is fast, and the lasting period is long.

In order to verify two kinds of active ingredients composite after synergistic effect, first applicant uses these two kinds of compositions to carry out indoor biometrics test, according to the mode of action of active ingredient, the raw object surveyed is decided to be thanatephorus cucumeris(frank) donk by us, this Pseudomonas Basidiomycotina fungi, sclerotium is dark brown, and bacterium colony is light brown to taupe mildew, can cause rice sheath blight disease.

The test method of indoor biometrics: reference standard NY/T1156.6-2006, adopts growth rate method to measure medicament to the inhibitory action of rice sheath blight disease pathogen mycelial growth.On the basis of preliminary experiment, from each medicament, design 5 concentration within the scope of 10%-90% are reached to germ mycelial growth inhibition rate, it is for subsequent use by the former medicine of 96% ZEN 90160 and the former medicine of 95% fluorobenzene ether acid amides with acetone to be first that solvent is made into the liquid of series concentration, then liquid is joined in 1% ratio in the PDA medium melting also cool to room temperature, after fully shaking up, pour in the culture dish (Φ 90mm) of sterilizing and make band medicine flat board, often process and repeat, to add the process of sterile water for blank for four times; With card punch (Φ 5mm) the picking Rhizoctonia solani Kuhn bacterium cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in the interior cultivation of constant incubator of 25 DEG C, adopt right-angled intersection method to measure colony diameter after cultivating 6d, calculate the EC of each medicament to pathogen ₅₀, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:

The theoretical toxicity index TTI=∑ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).

Be synergistic effect when co-toxicity coefficient CTC is greater than 120, being antagonism when being less than 80, is addition time between 80-120.

Table 1 ZEN 90160 and fluorobenzene ether acid amides and different ratio thereof are to the measurement result of rice sheath blight disease co-toxicity coefficient

Shown by above-mentioned data, when ZEN 90160 and fluorobenzene ether acid amides are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, has obvious synergistic effect to the control efficiency of rice sheath blight disease.

Tested by indoor biometrics, checking ZEN 90160 and fluorobenzene ether acid amides two kinds of active ingredients composite after, have good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 40.8% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example two, 51% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example three, 26% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example four, 13% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example five, 33% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example six, 44% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example seven, 12% ZEN 90160 fluorobenzene ether amide suspending agents

Example eight, 18% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example nine, 30% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Example ten, 20% ZEN 90160 fluorobenzene ether acid amides water dispersible granules

Select above example to carry out the field trial of the water prevention rice sheath blight, simultaneously by the Contrast on effect with ZEN 90160 and fluorobenzene ether acid amides single dose, verify composite after synergistic effect.Test site is peach of immortality area, Hubei Province, and the time is on July 25th, 2013, and test each process three repetition, community randomized arrangement, each plot area is 70m ², adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, records the synoptic data on the dispenser same day and a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is with reference to GB/T 17980.34-2000, each community diagonal 5 sampling, often some sampling 2-3 strain, investigate fixed 2 points of whole blade each community diagonal, often fixed 25 clumps of paddy rice of straight ahead, fixed point investigation in the 7th day, 21 days after dispenser, investigate each community disease to refer to, calculate preventive effect, and carry out the new multipole difference analysis of Deng Kenshi, calculate according to following grade scale:

0 grade: without scab;

1 grade: lesion area accounts for less than 5% of whole leaf area;

3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;

5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;

7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;

9 grades: lesion area accounts for more than 51% of whole leaf area, stem stalk there is scab.

Computing formula is as follows:

Table 3 30% ZEN 90160 fluorobenzene ether acid amides water dispersible granules and different agents are to the preventive effect of rice sheath blight disease

Analyze above-mentioned data to draw, ZEN 90160 and fluorobenzene ether acid amides composite after after medicine 7 days preventive effects all more than 70%, and 75% ZEN 90160 wetting powder, 7 days preventive effects after medicine are 43.50%, the preventive effect of 24% fluorobenzene ether amide suspending agents is 36.67%, visible, there is good synergistic effect after two kinds of active ingredients are composite.And after medicine 21 days, all complex preparations to be all better than after medicine 7 days to the preventive effect of rice sheath blight disease, and extend the lasting period, control efficiency is more lasting.

Claims

1. a bactericidal composition, is characterized in that: active component is made up of ZEN 90160 and fluorobenzene ether acid amides, and the weight ratio of described ZEN 90160 and fluorobenzene ether acid amides is 1:50 ~ 50:1.

2. bactericidal composition according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active component and auxiliary material.