CN103271094A - Compound bactericide - Google Patents
Compound bactericide Download PDFInfo
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- CN103271094A CN103271094A CN2013102449735A CN201310244973A CN103271094A CN 103271094 A CN103271094 A CN 103271094A CN 2013102449735 A CN2013102449735 A CN 2013102449735A CN 201310244973 A CN201310244973 A CN 201310244973A CN 103271094 A CN103271094 A CN 103271094A
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- tetramycin
- piperazine mycin
- bactericide
- shen piperazine
- disease
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Abstract
The invention belongs to the technical field of pesticides, and relates to a compound bactericide. The bactericide is characterized in that active ingredients comprise tetramycin and phenazino-1-carboxylic acid; and the weight ratio of tetramycin to phenazino-1-carboxylic acid is (1:100) to (100:1). The composition can be made into a suspending agent and aqueous solution, and is used for preventing rice sheath blight disease; the sterilizing effect is obvious; and the bactericide is safe for the plants.
Description
Technical field
The invention belongs to technical field of pesticide, contain the composite bactericide of tetramycin and Shen piperazine mycin specifically.
Background technology
Tetramycin is by a strain that separates in the soil sample of Wuzhou, the Guangxi stimulation metabolite of streptomyces hygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp) not.This product fungicidal spectrum is wide, and three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungi are all had extremely strong killing action.Be suitable for the control of the multiple fungal disease of various crops.This composition can obviously promote the callus healing simultaneously, promotes flourishing, the aging root system recovery of weak shoot root system, improves the crop disease-resistant ability and optimizes crop quality.
Shen piperazine mycin is by a kind of antibiotic of pseudomonas fluorescens through the biological culture secretion, has the dual-use function that wide spectrum suppresses phytopathogen and promotes plant growth effect simultaneously, this biological bactericide has wide spectrum, characteristics of high efficiency, can effectively prevent and treat fusarium wilt, climing rot, eqpidemic disease, banded sclerotial blight, false smut, rice blast, downy mildew, yellow rust, stalk break, head blight, anthracnose, gray mold, scab, leaf spot, bacterial wilt, canker, ginger plague and soil-borne disease soil treatment on the crops such as paddy rice, wheat, vegetables.
Rice sheath blight disease (Rhizoctorzia solani) claim moire disease again, is to be infected and fallen ill by Rhizoctonia solani Kuhn, how to fall ill under high temperature, super-humid conditions.Banded sclerotial blight is serious in the harm of south rice zone, is one of main disease on the current Rice Production.This disease can make paddy rice not ear, and particle weight descends, and has a strong impact on rice yield.
The agricultural chemicals that uses mostly is synthetic at present, and the intermediate that has a lot of high poison in building-up process produces, and the use day by day of a large amount of chemical pesticides has aggravated the influence to environment.At present present situation, people begin more to be conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between the microorganism to prevent and treat pathogen, this is than more efficient, the environmental protection of existing chemosynthesis composition, biogenic pesticide new varieties and the complex preparation of exploitation more and more are subject to people's attention at present, and two kinds of active ingredients that relate among the present invention are biogenic pesticide.We are composite with tetramycin and Shen piperazine mycin, in the hope of strengthening both drug effects, enlarge fungicidal spectrum, reduce peasant's labour cost.
Summary of the invention
The purpose of this invention is to provide a kind of composite bactericide with synergistic effect and safety and environmental protection, realize improving drug effect, reduce the purpose of polluting, reducing cost.Be specifically related to the composite bactericide of tetramycin and Shen piperazine mycin.
Utilize the described composite bactericide of the invention described above, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with those skilled in that art's known method, comprise aqua and suspending agent.
In the above-mentioned composite bactericide, the weight ratio of tetramycin and Shen piperazine mycin is 1:100～100:1.
Tetramycin and Shen piperazine mycin composite bactericide have following characteristics: 1. biogenic pesticide is little to environmental influence; 2. utilize the antagonism between the microorganism to come sterilization, effect is strong; 3. organic principle is not added in formulation environmental protection; 4. two kinds of compositions are composite, and synergistic effect is arranged; 5. twenty percent does not have cross resistance between dividing; 6. delay the generation of active ingredient resistance, reduce peasant's labour cost.
Embodiment
For determining tetramycin and two kinds of composite synergistic effects of active ingredient of Shen piperazine mycin, the applicant at first uses these two kinds of compositions to carry out the indoor biometrics test, and test method and result are as follows:
After at first having analyzed the effect characteristics of two kinds of active ingredients, the object that we will give birth to survey is decided to be thanatephorus cucumeris(frank) donk, this Pseudomonas Basidiomycotina fungi, and sclerotium is dark brown, and it is light brown to the taupe mildew that bacterium colony is, and this bacterium can cause the banded sclerotial blight of paddy rice.
The test method of indoor biometrics: adopt growth rate method to measure medicament to the inhibitory action of rice sheath blight disease mycelia growth.On the basis of preliminary experiment, from each medicament the germ mycelial growth inhibition rate is reached 5 concentration of design in 10%～90% scope, it is standby earlier with the former medicine of 95% tetramycin and the 70% Shen former medicine of piperazine mycin with methyl alcohol to be that solvent is made into the soup of series concentration, then with soup in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour into and make band medicine flat board in the culture dish (Φ 90mm) of sterilization, every processing repeats for four times, to add the blank that is treated to of sterile water; Card punch (Φ 5mm) picking Rhizoctonia solani Kuhn bacterium cake with sterilization, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in 25 ℃ interior cultivation of constant incubator, adopt the right-angled intersection method to measure colony diameter behind the cultivation 6d, calculate each medicament to the EC of pathogen
50, and calculate toxicity index according to the following equation, the virulence of more different medicaments and bacteriostasis.Used former medicine is tested in above-mentioned living test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
Co-toxicity coefficient CTC was synergistic effect greater than 120 o'clock, less than 80 o'clock be antagonism, be addition in the time of between 80-120.
The indoor biometrics test data sees the following form:
Table 1 tetramycin, Shen piperazine mycin and different proportioning are to the measurement result of rice sheath blight disease co-toxicity coefficient
By above-mentioned data as can be seen, when tetramycin and Shen piperazine mycin were composite in varing proportions, the co-toxicity coefficient of prescription (CTC) had obvious synergistic effect all greater than 120, wherein tetramycin and the Shen CTC of piperazine mycin between 1:5～5:1 are up to 165, and synergistic effect is extremely remarkable.
By indoor biometrics verification experimental verification tetramycin and two kinds of active ingredients of Shen piperazine mycin are composite good synergistic effect arranged, below by instantiation in order to further describing the present invention, but the present invention absolutely not only only limits to following these embodiment.Percentage all is weight percentage in the prescription, and employed former medicine and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 10.1% tetramycin Shen piperazine mycin suspending agent
Example three, 15.5% tetramycin Shen piperazine mycin suspending agent
Example four, 8.8% tetramycin Shen piperazine mycin aqua
Example five, 2% tetramycin Shen piperazine mycin suspending agent
Example six, 3% tetramycin Shen piperazine mycin aqua
Example seven, 3.3% tetramycin Shen piperazine mycin suspending agent
Example eight, 30.6% tetramycin Shen piperazine mycin aqua
Example nine, 20.2% tetramycin Shen piperazine mycin suspending agent
Select above example to prevent and treat the field trial of rice sheath blight disease, the prescription of determining is prevented and treated the effect of rice sheath blight disease; Simultaneously by with the contrast of the effect of 5% tetramycin SC and 5% Shen piperazine mycin SC, verify the synergistic effect after composite.
2% tetramycin Shen piperazine mycin suspending agent and other preparation are done the test of pesticide effectiveness of field control rice sheath blight disease.Test site is the Hanchuan City, Hubei Province, and the time is on June 15th, 2011, test each and handle three repetitions, and the residential quarter randomized arrangement, each residential quarter area is 60m
2, adopt the back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all residential quarters all planting habit with the locality is identical, records the dispenser same day and the synoptic data in a week thereafter, and is specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each residential quarter diagonal, and every some straight ahead is decided 25 clumps, and fixed point investigation in the 7th day, 21 days is investigated each residential quarter disease and is referred to after the dispenser, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
Table 3 2% tetramycin Shen piperazine mycin suspending agent and different medicament are to the preventive effect of rice sheath blight disease
Annotate: data are the mean value that repeats for 3 times in the table, and letter is all Duncan testing differentia remarkable time mutually: in June, 2011 place; Hanchuan, Hubei
From above-mentioned data as can be seen, tetramycin and Shen piperazine mycin complex preparation behind medicine 7 days preventive effects all above 70%, and the preventive effect that 5% tetramycin SC, 7 days preventive effects behind medicine are 46.24%, 5% Shen piperazine mycin SC is 42.60%, and preventive effect difference is extremely remarkable between visible complex preparation and the single agent.And behind medicine 21 days, complex preparation has all surpassed 80% to the preventive effect of rice sheath blight disease, and obviously, complex preparation can well improve drug effect, prolongs the lasting period, reduces peasant's labour cost, increases economic efficiency.
Claims (3)
1. composite bactericide, it is characterized in that: described active component is made up of tetramycin and Shen piperazine mycin, and the weight ratio of described tetramycin and Shen piperazine mycin is 1:100～100:1.
2. composite bactericide according to claim 1 is characterized in that: make aqua, suspending agent by active ingredient and auxiliary material.
3. according to claim 1 or the described composite bactericide of claim 2, it is characterized in that: be used for preventing and treating rice sheath blight disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102449735A CN103271094A (en) | 2013-06-19 | 2013-06-19 | Compound bactericide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102449735A CN103271094A (en) | 2013-06-19 | 2013-06-19 | Compound bactericide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103271094A true CN103271094A (en) | 2013-09-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102449735A Pending CN103271094A (en) | 2013-06-19 | 2013-06-19 | Compound bactericide |
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| Country | Link |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598192A (en) * | 2013-11-30 | 2014-02-26 | 海利尔药业集团股份有限公司 | Bactericidal composition containing tetramycin and zhongshengmycin |
| CN103609572A (en) * | 2013-11-30 | 2014-03-05 | 海利尔药业集团股份有限公司 | Sterilization composition containing tetramycin and amino-oligosaccharin |
| CN103636629A (en) * | 2013-11-28 | 2014-03-19 | 北京燕化永乐生物科技股份有限公司 | Polyoxin-containing bactericidal composition |
| CN103651430A (en) * | 2013-12-17 | 2014-03-26 | 北京燕化永乐生物科技股份有限公司 | Compound fungicide |
| CN103719114A (en) * | 2013-12-19 | 2014-04-16 | 北京燕化永乐生物科技股份有限公司 | Sterilization composition |
| CN103907644A (en) * | 2014-04-24 | 2014-07-09 | 北京燕化永乐生物科技股份有限公司 | Sterilization composite |
| CN104285945A (en) * | 2014-09-26 | 2015-01-21 | 郑州大学 | Phenazine-1-carboxylic acid (PCA) water suspension and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL6813094A (en) * | 1968-09-12 | 1970-03-16 | Tetramycin antibiotic | |
| CN101632367A (en) * | 2008-07-24 | 2010-01-27 | 辽宁省微生物科学研究院 | Application of biopesticide tetramycin in preventing and controlling Magnaporthe grisea |
| CN102812956A (en) * | 2012-09-18 | 2012-12-12 | 北京燕化永乐农药有限公司 | Bactericidal composition |
-
2013
- 2013-06-19 CN CN2013102449735A patent/CN103271094A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL6813094A (en) * | 1968-09-12 | 1970-03-16 | Tetramycin antibiotic | |
| CN101632367A (en) * | 2008-07-24 | 2010-01-27 | 辽宁省微生物科学研究院 | Application of biopesticide tetramycin in preventing and controlling Magnaporthe grisea |
| CN102812956A (en) * | 2012-09-18 | 2012-12-12 | 北京燕化永乐农药有限公司 | Bactericidal composition |
Non-Patent Citations (2)
| Title |
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| 余露: "生物农药——梧宁霉素", 《农药市场信息》 * |
| 温广月等: "新型微生物源农药申嗪霉素对水稻纹枯病菌的毒力测定及田间药效", 《植物保护》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103636629A (en) * | 2013-11-28 | 2014-03-19 | 北京燕化永乐生物科技股份有限公司 | Polyoxin-containing bactericidal composition |
| CN103598192A (en) * | 2013-11-30 | 2014-02-26 | 海利尔药业集团股份有限公司 | Bactericidal composition containing tetramycin and zhongshengmycin |
| CN103609572A (en) * | 2013-11-30 | 2014-03-05 | 海利尔药业集团股份有限公司 | Sterilization composition containing tetramycin and amino-oligosaccharin |
| CN103598192B (en) * | 2013-11-30 | 2016-01-06 | 海利尔药业集团股份有限公司 | A kind of bactericidal composition containing tetramycin and Zhongshengmycin |
| CN103609572B (en) * | 2013-11-30 | 2016-01-20 | 海利尔药业集团股份有限公司 | A kind of bactericidal composition containing tetramycin and amino-oligosaccharide |
| CN103651430A (en) * | 2013-12-17 | 2014-03-26 | 北京燕化永乐生物科技股份有限公司 | Compound fungicide |
| CN103651430B (en) * | 2013-12-17 | 2015-01-28 | 北京燕化永乐生物科技股份有限公司 | Compound fungicide |
| CN103719114A (en) * | 2013-12-19 | 2014-04-16 | 北京燕化永乐生物科技股份有限公司 | Sterilization composition |
| CN103719114B (en) * | 2013-12-19 | 2015-03-04 | 北京燕化永乐生物科技股份有限公司 | Sterilization composition |
| CN103907644A (en) * | 2014-04-24 | 2014-07-09 | 北京燕化永乐生物科技股份有限公司 | Sterilization composite |
| CN104285945A (en) * | 2014-09-26 | 2015-01-21 | 郑州大学 | Phenazine-1-carboxylic acid (PCA) water suspension and preparation method thereof |
| CN104285945B (en) * | 2014-09-26 | 2016-07-06 | 郑州大学 | A kind of shenqinmycin aqueous suspension agent and preparation method thereof |
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| C06 | Publication | ||
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| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant after: Beijing Yanhua Yongle Biotechnology Co., Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant before: Beijing Yoloo Pesticide Co., Ltd. |
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| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: BEIJING YOLOO PESTICIDE CO., LTD. TO: BEIJING YANHUA YONGLE BIOTECHNOLOGYCO., LTD. |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130904 |