CN103314970A - Bactericidal composition - Google Patents
Bactericidal composition Download PDFInfo
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- CN103314970A CN103314970A CN201310245343XA CN201310245343A CN103314970A CN 103314970 A CN103314970 A CN 103314970A CN 201310245343X A CN201310245343X A CN 201310245343XA CN 201310245343 A CN201310245343 A CN 201310245343A CN 103314970 A CN103314970 A CN 103314970A
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- tetramycin
- isoprothiolane
- rice blast
- bactericidal composition
- effect
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Abstract
The invention belongs to the technical field of pesticide and relates to a bactericidal composition. The bactericidal composition is characterized in that the active ingredients comprise tetramycin and isoprothiolane. The bactericidal composition is mainly used for preventing and treating rice blast and is excellent in bactericidal effect, obvious in synergistic effect and safe to rice.
Description
Technical field the invention belongs to technical field of pesticide, contains specifically the bactericidal composition with synergistic effect of tetramycin and Isoprothiolane.
Background technology
Tetramycin (Tetramycin) claim again tetramycin, is by a strain that separates in the soil sample of Wuzhou, the Guangxi stimulation metabolite of streptomyces hygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp.) not.This product fungicidal spectrum is wide, and three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungi are all had extremely strong killing action.The control of the applicable multiple fungal disease of various crops.Especially to fruit tree putrefaction disease, spot defoliation, cotton yellow (withered) wither disease, root rot, rice blast, seedling blight, ginseng and pseudo-ginseng black spot, tealeaves tea gall, fruit white rot of grape, the climing rot of watermelon, root rot, the rotten special efficacy of stem foot.This composition can obviously promote the callus healing simultaneously, promotes flourishing, the aging root system recovery of weak shoot root system, improves the crop disease-resistant ability and optimizes crop quality.
Isoprothiolane (Isoprothiolane) belongs to the low toxicity bactericide, has efficient interior absorption, is the special effect agent of control rice blast.Rice blast is had preventive and therapeutic action, can be absorbed by each position of paddy rice, and be accumulated to leaf section tissue, thus the performance drug effect, and resistance of rainwater washing against also can doublely be controlled plant hopper.Main control rice blast has certain preventive effect to rice blast, culm rot and bacterial leaf-blight simultaneously.
Rice blast (Magnaporthe grisea) rice blast is one of important disease of paddy rice, can cause the significant underproduction, the underproduction 40%～50% when serious, even No kernels or seeds are gathered, as in a year of scarcity.Each rice district, the world evenly occurs.This disease all has generation in various places, wherein occur as manyly with leaf section, joint section, can cause the in various degree underproduction after the generation, and especially panicle blast or joint pest occur early and weigh, can cause dead ears so that has no harvest.
The development difficulty of bactericide new varieties is larger; and at present because the abuse of bactericide causes existing a lot of sterilization components gradually to produce pesticide resistance; and single sterilization component is because mechanism of action is single; all diseases that usually can disposable elimination cover crop have so just strengthened peasant's labour cost.Show by field control effectiveness test, composite between the bactericide not only can alleviate pesticide resistance, can also improve drug effect.Simultaneously, bactericide compounded development cost can be alleviated the economic pressures of the new sterilization component of exploitation well below the development cost of new varieties, can receive preferably bactericidal effect again simultaneously.In sum, we are for the shortcoming of existing bactericide, and tetramycin and Isoprothiolane is composite, to strengthening both drug effects, enlarge fungicidal spectrum, reduce peasant's labour cost.
Summary of the invention
The purpose of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize improving drug effect, reduce the purpose of polluting, reducing cost.Be specifically related to the bactericidal composition of tetramycin and Isoprothiolane.
Utilize bactericidal composition of the present invention, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise aqueous emulsion and water dispersible granules.
In the above-mentioned microbicide compositions, the weight ratio of tetramycin and Isoprothiolane is 1:100～50:1.
Tetramycin and Isoprothiolane composite bactericide have following characteristics: 1. formulation environmental protection, use less organic principle, and environmentally safe; 2. two kinds of compositions are composite, and synergistic effect is arranged; 3. between twenty percent divides without cross resistance; 4. delay the generation of active ingredient resistance, reduce peasant's labour cost.
Embodiment
For determining tetramycin and two kinds of composite synergistic effects of active ingredient of Isoprothiolane, the applicant at first uses these two kinds of compositions to carry out the indoor biometrics test, and test method and result are as follows:
After at first having analyzed the effect characteristics of two kinds of active ingredients, the object that we will give birth to survey is decided to be Magnaporthe grisea (Phyricularia grisea Sacc.), this Pseudomonas Deuteromycotina fungi, and this bacterium can cause the rice blast of paddy rice.
The test method of indoor biometrics: adopt growth rate method to measure medicament to the inhibitory action of rice blast mycelia growth.On the basis of preliminary experiment, from each medicament the germ mycelial growth inhibition rate is reached 5 concentration of design in 10%～90% scope, the liquid that first the former medicine of 95% tetramycin and 95% isoprothiolane is made into series concentration take acetone as solvent is for subsequent use, then with liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that to make the band medicine in the culture dish (Φ 90mm) of sterilization dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of same amount acetone; Card punch (Φ 5mm) picking rice blast fungus bacterium cake with sterilization, mycelia faces down aseptic inoculation in the central authorities with the medicine flat board, be inverted in 25 ℃ interior cultivation of constant incubator, cultivate and adopt the right-angled intersection method to measure colony diameter after 6 days, calculate each medicament to the EC of pathogen
50, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Used former medicine is tested in above-mentioned living test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
Co-toxicity coefficient CTC was synergistic effect greater than 120 o'clock, less than 80 o'clock be antagonism, be addition in the time of between 80～120.
The indoor biometrics experimental data sees the following form:
Table 1 tetramycin, Isoprothiolane and different proportioning are to the measurement result of rice blast co-toxicity coefficient
Analyze above-mentioned data, can obviously find out when tetramycin and Isoprothiolane are composite in varing proportions, the co-toxicity coefficient (CTC) of prescription has stronger synergistic effect all greater than 130 to the preventive effect of rice blast, and wherein the synergy between 1:5～5:1 of tetramycin and Isoprothiolane is the most remarkable.
Determine tetramycin and two kinds of active ingredients of Isoprothiolane are composite that good synergistic effect can be arranged by indoor biometrics test, below by instantiation in order to further describing the present invention, but the present invention absolutely not only only limits to following these embodiment.Percentage all is weight percentage in the prescription, and employed former medicine and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 20.2% tetramycin isoprothiolane emulsion in water
Example two, 13% tetramycin isoprothiolane emulsion in water
Example three, 15.3% tetramycin isoprothiolane emulsion in water
Example four, 12% tetramycin isoprothiolane emulsion in water
Example five, 6% tetramycin isoprothiolane emulsion in water
Example six, 24% tetramycin 'Daowenling ' water dispersible granule
Example seven, 33% tetramycin 'Daowenling ' water dispersible granule
Example eight, 51% tetramycin 'Daowenling ' water dispersible granule
Example nine, 26% tetramycin 'Daowenling ' water dispersible granule
Select above nine field trials that example is prevented and treated rice blast, to determine the effect of prescription control rice blast; Simultaneously by with the Contrast on effect of tetramycin single dose and Isoprothiolane single dose, verify the synergistic effect after composite.Concrete test method data are as follows:
6% tetramycin isoprothiolane emulsion in water and other preparation are done the test of pesticide effectiveness of field control rice blast.Test site is the Yiyang Prefecture, Hunan Province, and the time is on June 12nd, 2012, test each and process three repetitions, and the residential quarter randomized arrangement, each residential quarter area is 90m
2, adopt the back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all residential quarters all planting habit with the locality is identical, records the dispenser same day and the synoptic data in a week thereafter, and is specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each residential quarter diagonal, and every some straight ahead is decided 25 clumps, and fixed point investigation in the 7th day, 21 days is investigated each residential quarter disease and is referred to after the dispenser, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
Table 3 6% tetramycin isoprothiolane emulsion in water and different medicament are to the preventive effect of rice blast
Annotate: data are the mean value that repeats for 3 times in the table, and letter is all Duncan testing differentia remarkable time mutually: in June, 2012 place; Yiyang, hunan
As can be seen from the above table, tetramycin and Isoprothiolane complex preparation behind medicine 7 days preventive effects all above 80%, and 5% tetramycin SC, 7 days preventive effects behind medicine only are 52.95%, the preventive effect of 5% Isoprothiolane EC is 56.01%, compare 5% tetramycin SC and 5% Isoprothiolane EC, preventive effect difference is extremely remarkable, and visible complex preparation has good synergistic effect.And behind medicine 21 days, complex preparation has all surpassed 85% to the preventive effect of rice blast, and compares in 7 days behind the medicine, preventive effect is higher, and the lasting period of composite rear preparation prolongs, and is more lasting to the control efficiency of rice blast, can alleviate peasant's the amount of labour, very high practicality is arranged.
Claims (3)
1. bactericidal composition, it is characterized in that: described active component is comprised of tetramycin and Isoprothiolane, and the weight ratio of tetramycin and Isoprothiolane is 1:100～50:1.
2. composition pesticide according to claim 1 is characterized in that: make aqueous emulsion, water dispersible granules by active ingredient and auxiliary material.
3. it is characterized in that according to claim 1 or composition pesticide claimed in claim 2: be used for the control rice blast.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310245343XA CN103314970A (en) | 2013-06-19 | 2013-06-19 | Bactericidal composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310245343XA CN103314970A (en) | 2013-06-19 | 2013-06-19 | Bactericidal composition |
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| CN103314970A true CN103314970A (en) | 2013-09-25 |
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| CN201310245343XA Pending CN103314970A (en) | 2013-06-19 | 2013-06-19 | Bactericidal composition |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103719113A (en) * | 2013-12-16 | 2014-04-16 | 骆毛喜 | Pesticide water solution and preparing method and application thereof |
| CN104621147A (en) * | 2013-11-26 | 2015-05-20 | 辽宁微科生物工程有限公司 | Tetramycin compound and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101069505A (en) * | 2007-06-21 | 2007-11-14 | 王建军 | Sterilizing composition containing kasugamycin and isoprothiolane |
| CN101632367A (en) * | 2008-07-24 | 2010-01-27 | 辽宁省微生物科学研究院 | Application of biopesticide tetramycin in preventing and controlling Magnaporthe grisea |
-
2013
- 2013-06-19 CN CN201310245343XA patent/CN103314970A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101069505A (en) * | 2007-06-21 | 2007-11-14 | 王建军 | Sterilizing composition containing kasugamycin and isoprothiolane |
| CN101632367A (en) * | 2008-07-24 | 2010-01-27 | 辽宁省微生物科学研究院 | Application of biopesticide tetramycin in preventing and controlling Magnaporthe grisea |
Non-Patent Citations (1)
| Title |
|---|
| 韩润亭等: "生物农药梧宁霉素防治水稻稻瘟病田间药效试验", 《现代农业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621147A (en) * | 2013-11-26 | 2015-05-20 | 辽宁微科生物工程有限公司 | Tetramycin compound and application |
| CN103719113A (en) * | 2013-12-16 | 2014-04-16 | 骆毛喜 | Pesticide water solution and preparing method and application thereof |
| CN103719113B (en) * | 2013-12-16 | 2015-11-18 | 骆毛喜 | A kind of aqueous pesticide solution and preparation method thereof and application |
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Address after: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant after: Beijing Yanhua Yongle Biotechnology Co., Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Applicant before: Beijing Yoloo Pesticide Co., Ltd. |
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Free format text: CORRECT: APPLICANT; FROM: BEIJING YOLOO PESTICIDE CO., LTD. TO: BEIJING YANHUA YONGLE BIOTECHNOLOGYCO., LTD. |
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Application publication date: 20130925 |