CN103636629A - Polyoxin-containing bactericidal composition - Google Patents

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a polyoxin-containing bactericidal composition which is characterized in that the active ingredients include tetramycin and polyoxin at a weight ratio of (1:50)-(50:1). The composition can be made into a suspending agent for preventing rice sheath blight, realizes a remarkable synergistic effect and is environment-friendly.

Description

Bactericidal composition containing polyoxin

Technical field

The invention belongs to technical field of pesticide, contain specifically the bactericidal composition with synergistic effect of tetramycin and polyoxin.

Background technology

Tetramycin (Tetramycin) claim again tetramycin, is the not stimulation metabolite of streptomyces hygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp) of the strain that separates in the soil sample of Wuzhou, Guangxi.This product fungicidal spectrum is wide, and three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungi are all had to extremely strong killing action.The control of the applicable multiple fungal disease of various crops.Especially to fruit tree putrefaction disease, spot defoliation; Cotton yellow (withered) disease of withering; Root rot; Rice sheath blight disease, seedling blight; Ginseng and pseudo-ginseng black spot; Tealeaves tea gall; Fruit white rot of grape, the climing rot of watermelon, root rot, the rotten special efficacy of stem foot.This composition can obviously promote callus healing simultaneously, promotes weak seedling well developed root system, the recovery of aging root system, improves crop disease-resistant ability and optimizes crop quality.

Polyoxin (polyoxin) is the metabolite that streptomyces aureus produces, and belongs to broad-spectrum antibiotics series bactericidal agent.In having preferably, inhale conduction.Its mechanism of action is to disturb the chitinous biosynthesis of germ cell wall, makes somatic cells wall can not carry out biosynthesis and causes germ dead.After germ tube contacts medicament with mycelia, cell inclusion is expanded, breaks, overflowed in part, and can not normal development, causes death.Therefore also there is the effect that germ produces spore and scab expansion that suppresses.Because polyoxin is a kind of efficient, low toxicity, the safety pesticide of non-environmental-pollution, so be widely used in cereal crops, special crop, the control of the important disease such as fruits and vegetables.

The agricultural chemicals using mostly at present is synthetic, the intermediate that has a lot of high poison in building-up process produces, these high malicious intermediates are very large to environmental influence, for current present situation, people start to be more conceived to the more free of contamination biogenic pesticide of low toxic and environment-friendly, because biogenic pesticide is to utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition.We are composite by tetramycin and polyoxin, to strengthening both drug effects, expand fungicidal spectrum, reduce peasant's labour cost.

Summary of the invention

The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the bactericidal composition that contains tetramycin and polyoxin.

Utilize bactericidal composition of the present invention, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make suspending agent of the present invention.

In above-mentioned bactericidal composition, the weight ratio of tetramycin and polyoxin is 1:50-50:1.

Bactericidal composition has following characteristics: 1. active ingredient is biogenic, safety and environmental protection; 2. utilize the antagonism between microorganism to carry out sterilization, ability to function is strong; 3. formulation environmental protection, does not add organic principle, easy to use; 4. two kinds of active ingredients are composite, also have synergistic effect; 5. twenty percent divide between without cross resistance; 6. delay the generation of active ingredient resistance, reduce peasant's labour cost.

Embodiment

For determining tetramycin and two kinds of composite synergistic effects of active ingredient of polyoxin, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:

First analyzed after the effect feature of two kinds of active ingredients, we are decided to be thanatephorus cucumeris(frank) donk (Thanatephorus cucumeris) by the raw object of surveying, this Pseudomonas Basidiomycotina fungi, and sclerotium is dark brown, it is light brown to taupe mildew that bacterium colony is, and this bacterium can cause the banded sclerotial blight of paddy rice.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of rice sheath blight disease mycelia.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of 95% tetramycin and the former medicine of 70% polyoxin be take methyl alcohol as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Card punch (Φ 5mm) picking Rhizoctonia solani Kuhn bacterium cake with sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen ₅₀, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).

It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.

Indoor biometrics test data sees the following form:

Table 1 tetramycin, polyoxin and the measurement result of different proportioning to rice sheath blight disease co-toxicity coefficient thereof

Analyze above-mentioned data, can obviously find out when tetramycin and polyoxin are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the control of rice sheath blight disease is had to the synergistic effect of different proportion, wherein tetramycin and polyoxin are being that proportioning five is the most remarkable to the synergy between proportioning seven between 1:5-5:1, the composite desirable medicament of preventing and treating rice sheath blight disease that becomes of tetramycin and polyoxin.

By indoor biometrics test determine tetramycin and two kinds of active ingredients of polyoxin composite, there is good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 51% tetramycin polyoxin suspending agent

Example two, 15.5% tetramycin polyoxin suspending agent

Example three, 22% tetramycin polyoxin suspending agent

Example four, 18% tetramycin polyoxin suspending agent

Example five, 10% tetramycin polyoxin suspending agent

Example six, 25.5% tetramycin polyoxin suspending agent

Example seven, 31% tetramycin polyoxin suspending agent

Example eight, 33% tetramycin polyoxin suspending agent

Example nine, 22% tetramycin polyoxin suspending agent

Select above example to prevent and treat the field trial of rice sheath blight disease, the formula of determining is prevented and treated the effect of rice sheath blight disease; Simultaneously by the Contrast on effect with tetramycin and polyoxin single dose, verify the synergistic effect after composite.Concrete test method data are as follows:

10% tetramycin polyoxin suspending agent and other preparation are done the test of pesticide effectiveness of field control rice sheath blight disease.Test site is Hanchuan City, Hubei Province, and the time is on June 10th, 2012, test each and process three repetitions, and community randomized arrangement, each community area is 100m ², adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.

The grade scale of disease index:

0 grade: without scab;

1 grade: lesion area accounts for below 5% of whole leaf area;

3 grades: lesion area accounts for 6%～10% of whole leaf area;

5 grades: lesion area accounts for 11%～25% of whole leaf area;

7 grades: lesion area accounts for 26%～50% of whole leaf area;

9 grades: lesion area accounts for the more than 51% of whole leaf area.

Table 310% tetramycin polyoxin suspending agent and the preventive effect of different medicament to rice sheath blight disease

Note: in table, data are the mean value time repeating for 3 times: in June, 2012 place; Hanchuan, Hubei

From above-mentioned data, can find out, tetramycin and polyoxin complex preparation after medicine 7 days preventive effects all over 75%, and tetramycin single dose 7 days preventive effects after medicine are 47.39%, the preventive effect of polyoxin single dose is 41.72%, compare tetramycin and polyoxin single dose, the synergistic effect of complex preparation is extremely remarkable, after visible two kinds of active ingredients are composite, has good synergistic effect.And after medicine 21 days, all complex preparations have all surpassed 80% to the preventive effect of rice sheath blight disease, and visible complex preparation has not only extended the lasting period, strengthens drug effect, and has alleviated peasant's drug cost, environmentally friendly.

Claims (3)

1. containing the bactericidal composition of polyoxin, it is characterized in that: active component is comprised of tetramycin and polyoxin, the weight ratio of described tetramycin and polyoxin is 1:50～50:1.

2. bactericidal composition according to claim 1, is characterized in that: by active ingredient and auxiliary material, make suspending agent.

3. according to claim 1 or bactericidal composition claimed in claim 2, it is characterized in that: for preventing and treating rice sheath blight disease.