CN103719114B - Sterilization composition - Google Patents

Abstract

The invention belongs to the technical field of pesticides, and relates to a sterilization composition. The sterilization composition is characterized in that an active ingredient contains tetramycin and jiean-peptide. The sterilization composition can be prepared into water dispersible granules or a suspending agent. The sterilization composition has an obvious synergism effect and a long lasting period, and is environmentally friendly.

Description

Bactericidal composition

Technical field

The invention belongs to technical field of pesticide, specifically containing tetramycin and Jiean-peptide bactericidal composition.

Background technology

Tetramycin (Tetramycin), also known as tetramycin, is by the stimulation metabolite of the strain S. ahygroscopicus Wuzhou subspecies (Streptomyces ahygroscopicn wuzhouensisn subsp.) separated in the soil sample of Wuzhou, Guangxi.This product fungicidal spectrum is wide, all has extremely strong killing action to three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungies.Be suitable for the control of the multiple fungal disease of various crops.Especially to fruit tree putrefaction disease, spot defoliation; Cotton yellow (withered) withers disease; Root rot; Cotton verticillium wilt, seedling blight; Ginseng and Alternaria panax; Tealeaves tea gall; Fruit white rot of grape, watermelon grafting, root rot, brown foot rot special efficacy.This composition can obviously promote that callus heals simultaneously, promotes that weak seedling well developed root system, aging root system are recovered, and improves crop disease-resistant ability and optimizes crop quality.

Jiean-peptide (jiean-peptide) derives from the metabolite of bacillus, all has stronger Developing restraint effect and control efficiency to pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium.Field test results shows: to tomato and Botrytis cinerea, capsicum damping off, cucumber, wheat powdery mildew etc., and control efficiency is remarkable, reaches more than 85%, is a kind of novel biological bactericide having research and development value and application prospect, has good market prospects.

Cotton verticillium wilt is one of the Major Diseases of Cotton Production of causing harm.Under natural conditions there is symptom in seedling morbidity less or seldom.Generally start aobvious disease at 3 ~ 5 leaf periods, after the middle and late growth stage cotton buddings, fall ill in a large number in field, the leaf margin just in plant lower blade and occur light yellow patch between vein, after expand gradually, leaf look chlorosis shoals, and master pulse and surrounding thereof still keep green, sick leaf occurs that palmate is mottled, mesophyll is thickening, and leaf margin is curling downwards, and blade from bottom to top comes off gradually, only surplus top minority leaflet, cotton buds and bolls are rare, and cotton boll ftractures in advance, and later stage diseased plant base portion bears tiny shoot.The sick stem of vertical profile, xylem produces light brown variable color striped.Occur acute wilting symptom after Summer Heavy Rainfall, cotton plant withers suddenly vertical, and blade comes off in a large number, and serious plot of falling ill is so tragic that one cannot bear to look at it, causes Severe Reduction.

The tunning of biogenic pesticide owing to being microorganism, in building-up process, do not have the intermediate of a lot of high poison to produce, and due to biogenic pesticide be utilize the antagonist properties between microorganism to prevent and treat pathogen, this is more efficient and environmental protection than existing chemosynthesis composition, new varieties and the complex preparation of current exploitation biogenic pesticide are more and more subject to people's attention, and relate in the present invention two kinds of active ingredients are biogenic pesticide.We by tetramycin and Jiean-peptide composite, to the drug effect both strengthening, expand fungicidal spectrum, reduce the labour cost of peasant.

Summary of the invention

The object of this invention is to provide and a kind ofly there is synergistic effect and the bactericidal composition of safety and environmental protection, realize improving drug effect, decreasing pollution, the object that reduces costs.Be specifically related to tetramycin and the composite bactericidal composition of Jiean-peptide.

Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise suspending agent and water dispersible granules.

In above-mentioned bactericidal composition, the weight ratio of tetramycin and Jiean-peptide is 1:60-60:1.

Above-mentioned bactericidal composition has following characteristics: 1. sterilizing ability is strong; 2. former medicine is safe and efficient; 3. formulation environmental protection; 4. composite have synergistic effect; 5. between two kinds of compositions without cross resistance.

Embodiment

For determining tetramycin and the composite synergistic effect of Jiean-peptide two kinds of active ingredients, first applicant uses these two kinds of compositions to carry out indoor biometrics test, test method and result as follows:

Dahlia wheel branch spore (Verticillium dahliae Kleb.) is the main pathogenic fungi causing cotton verticillium wilt, and according to the mechanism of action of two kinds of active ingredients, life is surveyed this bacterium of objects location by us.

The test method of indoor biometrics: adopt the inhibitory action that growth rate method mensuration medicament grows cotton verticillium wilt mycelia.On the basis of preliminary experiment, from each medicament, design 5 concentration within the scope of 10%-90% are reached to germ mycelial growth inhibition rate, first by former for the tetramycin of 70% medicine and 70% the former medicine of Jiean-peptide with methyl alcohol to be that solvent is made into the liquid of series concentration for subsequent use, then liquid is joined in 1% ratio in the PDA medium melting also cool to room temperature, after fully shaking up, pour in the culture dish (Φ 90mm) of sterilizing and make band medicine flat board, often process and repeat, to add the process of sterile water for blank for four times; With card punch (Φ 5mm) the picking verticillium dahliae cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in the interior cultivation of constant incubator of 25 DEG C, right-angled intersection method is adopted to measure colony diameter after cultivating 6d, calculate the EC50 of each medicament to pathogen, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:

The theoretical toxicity index TTI=∑ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).

Be synergistic effect when co-toxicity coefficient CTC is greater than 120, being antagonism when being less than 80, is addition time between 80-120.

Indoor biometrics test data sees the following form:

Table 1 tetramycin, Jiean-peptide and different ratio thereof are to the measurement result of cotton verticillium wilt co-toxicity coefficient

Analyze above-mentioned data, can obviously find out when tetramycin and Jiean-peptide are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of cotton verticillium wilt is had to the synergistic effect of different proportion, wherein the synergy between 1:5-5:1 namely between proportioning five to proportioning seven of tetramycin and Jiean-peptide is visible, the composite favourable agents becoming control cotton verticillium wilt of tetramycin and Jiean-peptide.

Determine tetramycin by indoor biometrics test and Jiean-peptide two kinds of active ingredients are composite can good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 61% tetramycin Jiean-peptide water dispersible granules

Example two, 31.5% tetramycin Jiean-peptide suspending agent

Example three, 22% tetramycin Jiean-peptide suspending agent

Example four, 18% tetramycin Jiean-peptide suspending agent

Example five, 10% tetramycin Jiean-peptide suspending agent

Example six, 12% tetramycin Jiean-peptide water dispersible granules

Example seven, 44% tetramycin Jiean-peptide suspending agent

Example eight, 30.5% tetramycin Jiean-peptide suspending agent

Example nine, 24% tetramycin Jiean-peptide suspending agent

Example ten, 15% tetramycin Jiean-peptide suspending agent

Example 11,10% tetramycin Jiean-peptide suspending agent

Select above example to carry out preventing and treating the field trial of cotton verticillium wilt, determine to fill a prescription and prevent and treat the effect of cotton verticillium wilt; Simultaneously with the Contrast on effect of tetramycin and Jiean-peptide single dose, verify composite after synergistic effect.Concrete test method data are as follows:

The field control effectiveness test of 10% tetramycin Jiean-peptide suspending agent and other preparation control cotton verticillium wilt.Test site is Xinyang City, Henan Province, and the time is on July 25th, 2012, and test each process three repetition, community randomized arrangement, each plot area is 100m ², adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, records the synoptic data on the dispenser same day and a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: fixed 2 points of each community diagonal, and often some straight ahead determines 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refer to, calculate preventive effect, and carry out the new multipole difference analysis of Deng Kenshi.

The grade scale of disease index:

0 grade: healthy tree, blade is without symptom;

The blade performance symptom of 1 grade: less than 10%;

The blade performance symptom of 3 grades: less than 25%, mesophyll becomes faint yellow or has difform yellow scab;

The blade of 5 grades: 26% ~ 50% shows symptom, and scab color major part is yellow or yellowish-brown, and blade edge is slightly rolled up withered;

The blade performance symptom of 7 grades: 51% ~ 75%, has minority blade to wither and fall;

9 grades: 76% with blade morbidity, and mostly be the withered spot of brown palmate, what blade had comes off, and time serious, even whole strain is withered.

Table 310% tetramycin Jiean-peptide suspending agent and different agents are to the preventive effect of cotton verticillium wilt

As can be seen from above-mentioned data, tetramycin and Jiean-peptide complex preparation after medicine 7 days preventive effects all more than 77%, and tetramycin single dose 7 days preventive effects after medicine are 54.27%, the preventive effect of Jiean-peptide single dose is 59.76%, it is extremely remarkable that complex preparation compares single dose synergy, has good synergistic effect after visible two kinds of active ingredients are composite.And after medicine 21 days, all complex preparations have all exceeded 83% to the preventive effect of cotton verticillium wilt, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of cotton verticillium wilt.Complex preparation can well improve drug effect, extends the lasting period, alleviates the amount of labour of peasant, have very high practicality.

Claims (3)

1. a bactericidal composition, is characterized in that: active component is made up of tetramycin and Jiean-peptide, and the weight ratio of described tetramycin and Jiean-peptide is 1:60 ~ 60:1.

2. bactericidal composition according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active component and auxiliary material.

3. according to bactericidal composition according to claim 1 or claim 2 for preventing and treating the purposes of cotton verticillium wilt.