CN103651397B - Compound bactericide - Google Patents
Compound bactericide Download PDFInfo
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- CN103651397B CN103651397B CN201310701475.9A CN201310701475A CN103651397B CN 103651397 B CN103651397 B CN 103651397B CN 201310701475 A CN201310701475 A CN 201310701475A CN 103651397 B CN103651397 B CN 103651397B
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- jiean
- peptide
- tebuconazole
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- wheat
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Abstract
The invention belongs to the technical field of pesticides, and relates to a compound bactericide. The compound bactericide is characterized in that active ingredients comprise Jiean-peptide and penconazole. Composition can be prepared into a suspension agent and water dispersible granules. The bactericidal spectrum is wide, the synergistic effect is remarkable, and the compound bactericide is environment-friendly.
Description
Technical field
The invention belongs to technical field of pesticide, contain the composite bactericide with synergistic effect of Jiean-peptide and Tebuconazole specifically.
Background technology
Jiean-peptide (jiean-peptide) derives from the metabolite of bacillus, all has stronger Developing restraint effect and control efficiency to pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium.Field test results shows: to tomato and Botrytis cinerea, capsicum damping off, cucumber, wheat powdery mildew etc., and control efficiency is remarkable, reaches more than 85%, is a kind of novel biological bactericide having research and development value and application prospect, has good market prospects.
Tebuconazole (Tebuconazole) a kind of efficient, wide spectrum, interior absorption triazole type sterilization pesticide, have protection, treat, root out three zones, fungicidal spectrum is wide, the lasting period is long.This composition is the same with all triazole bactericidal agents, and Tebuconazole can the biosynthesis of ergosterol of Antifungi.Tebuconazole is mainly used in preventing and treating the multiple fungal disease on the crops such as wheat, paddy rice, peanut, vegetables, banana, apple, pears and corn and Chinese sorghum, and it obtains registration and extensive use in 60 various crop of more than 50 countries in the whole world.
Wheat sharp eyespot has become China's wheat district regular incidence evil.After wheat infects by the withered bacterium of line, there is the symptoms such as rotten bud, sick seedling are withered, the rotten stem of flower stalk, withered strain dead ears at each growing stage.Jiean-peptide and Tebuconazole have good preventive effect to wheat sharp eyespot, after composite for two kinds of compositions, have good synergistic effect.
Summary of the invention
The object of this invention is to provide and a kind ofly there is synergistic effect and the composite bactericide of safety and environmental protection, realize improving drug effect, decreasing pollution, the object that reduces costs.Be specifically related to the composite bactericide of Jiean-peptide and Tebuconazole.
Utilize above-mentioned composite bactericide, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise suspending agent and water dispersible granules.
In above-mentioned composite bactericide, the weight ratio of Jiean-peptide and Tebuconazole is 1:60-60:1.
Above-mentioned composite bactericide has following characteristics: 1. active ingredient is biogenic, low toxic and environment-friendly; 2. both are composite has synergistic effect; 3. both are without cross resistance; 4. sterilizing ability is strong; 5. formulation environmental protection.
Embodiment
For determining Jiean-peptide and the composite synergistic effect of Tebuconazole two kinds of active ingredients, first applicant uses these two kinds of compositions to carry out indoor biometrics test, test method and result as follows:
Beak angle load bacterium (Ceratobasidium cornigerum Rogers) belongs to Basidiomycotina fungi, is the main pathogenic fungi causing wheat sharp eyespot, and to wheat sharp eyespot preventive effect very well, life is surveyed object and is decided to be this bacterium by us for Jiean-peptide and Tebuconazole.
The test method of indoor biometrics: adopt growth rate method to measure medicament to the inhibitory action of the mycelial growth of Etiology of Sharp Eyespot of Wheat bacterium.On the basis of preliminary experiment, from each medicament, design 5 concentration within the scope of 10%-90% are reached to germ mycelial growth inhibition rate, first by former for the Jiean-peptide of 70% medicine and 95% Original Tebuconazole with acetone to be that solvent is made into the liquid of series concentration for subsequent use, then liquid is joined in 1% ratio in the PDA medium melting also cool to room temperature, after fully shaking up, pour in the culture dish (Φ 90mm) of sterilizing and make band medicine flat board, often process and repeat, to add the process of sterile water for blank for four times; With the bacterium cake of card punch (Φ 5mm) the picking Etiology of Sharp Eyespot of Wheat bacterium of sterilizing, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in the interior cultivation of constant incubator of 25 DEG C, adopt right-angled intersection method to measure colony diameter after cultivating 6d, calculate the EC of each medicament to pathogen
50, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:
The theoretical toxicity index TTI=∑ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).
Be synergistic effect when co-toxicity coefficient CTC is greater than 120, being antagonism when being less than 80, is addition time between 80-120.
Table 1 Jiean-peptide, Tebuconazole and different ratio thereof are to the measurement result of wheat sharp eyespot co-toxicity coefficient
Analyze above-mentioned data, can obviously find out when Jiean-peptide and Tebuconazole are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of Etiology of Sharp Eyespot of Wheat bacterium is had to the synergistic effect of different proportion, wherein the synergy between 1:5-5:1 namely between proportioning five to proportioning seven of Jiean-peptide and Tebuconazole is the most remarkable, the composite favourable agents becoming control wheat sharp eyespot of visible Jiean-peptide and Tebuconazole.
Determine Jiean-peptide by indoor biometrics test and Tebuconazole two kinds of active ingredients are composite can good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 61% Jiean-peptide tebuconazole water dispersible granule
Example two, 42% Jiean-peptide tebuconazole suspension concentrates
Example three, 33% Jiean-peptide tebuconazole suspension concentrates
Example four, 24% Jiean-peptide tebuconazole water dispersible granule
Example five, 10% Jiean-peptide tebuconazole suspension concentrates
Example six, 30% Jiean-peptide tebuconazole suspension concentrates
Example seven, 22% Jiean-peptide tebuconazole suspension concentrates
Example eight, 16.8% Jiean-peptide tebuconazole suspension concentrates
Example nine, 30.5% Jiean-peptide tebuconazole suspension concentrates
Example ten, 15% Jiean-peptide tebuconazole suspension concentrates
Example 11,20% Jiean-peptide tebuconazole suspension concentrates
Select above example to carry out preventing and treating the field trial of wheat sharp eyespot, determine to fill a prescription and prevent and treat the effect of wheat sharp eyespot; Simultaneously by the Contrast on effect with Jiean-peptide and Tebuconazole single dose, verify composite after synergistic effect.Concrete test method data are as follows:
20% Jiean-peptide tebuconazole suspension concentrates and other preparation do the test of pesticide effectiveness of field control wheat sharp eyespot.Test site is Zhumadian prefecture, Henan province city, and the time is on April 16th, 2012, and test each process three repetition, community randomized arrangement, each plot area is 100m
2, adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, records the synoptic data on the dispenser same day and a week thereafter, specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each community diagonal, and often some straight ahead determines 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refer to, calculate preventive effect, and carry out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade: healthy tree, blade is without symptom
1 grade: symptom blade accounts for institute vaned less than 5%
3 grades: symptom blade accounts for vaned 6%-15%
5 grades: symptom blade accounts for vaned 16%-25%
7 grades: symptom blade accounts for vaned 26%-50%
9 grades: symptom blade accounts for institute vaned more than 50%
Table 315% Jiean-peptide tebuconazole suspension concentrates and different agents are to the preventive effect of wheat sharp eyespot
As can be seen from above-mentioned data, Jiean-peptide and Tebuconazole complex preparation after medicine 7 days preventive effects all more than 72%, and Jiean-peptide single dose 7 days preventive effects after medicine are 52.75%, the preventive effect of Tebuconazole single dose is 51.43%, compare Jiean-peptide and Tebuconazole single dose, have the preventive effect of pole significant difference between complex preparation and single dose, visible two active ingredients are composite good synergistic effect.And after medicine 21 days, all complex preparations have all exceeded 80% to the preventive effect of wheat sharp eyespot, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of wheat sharp eyespot.Can drug effect be improved after two-component is composite, extend the lasting period, there is good economic benefit.
Claims (2)
1. a composite bactericide, is characterized in that: active component is made up of Jiean-peptide and Tebuconazole, and the weight ratio of described Jiean-peptide and Tebuconazole is 1:60 ~ 60:1.
2. composite bactericide according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active component and auxiliary material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701475.9A CN103651397B (en) | 2013-12-18 | 2013-12-18 | Compound bactericide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701475.9A CN103651397B (en) | 2013-12-18 | 2013-12-18 | Compound bactericide |
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| CN103651397A CN103651397A (en) | 2014-03-26 |
| CN103651397B true CN103651397B (en) | 2015-01-07 |
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| CN201310701475.9A Active CN103651397B (en) | 2013-12-18 | 2013-12-18 | Compound bactericide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE1339287U (en) * | ||||
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| CN100399889C (en) * | 2005-05-13 | 2008-07-09 | 胡玉莲 | High-tension winding ceramic disc of electronic insects killing lamp |
| WO2010046927A2 (en) * | 2008-10-16 | 2010-04-29 | Deepak Pranjivandas Shah | Use of synergistic composition |
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Inventor after: Yang Huiying Inventor after: Liu Xiaojiao Inventor after: Ma Songyue Inventor after: Shen Dongxia Inventor after: Luo Xueting Inventor after: Li Qiumei Inventor after: Xu Bo Inventor before: Yang Huiying Inventor before: Liu Xiaojiao Inventor before: Wu Yong Inventor before: Ma Songyue Inventor before: Wang Zhandi |
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Effective date of registration: 20171221 Address after: 063600 Leting County Economic Development Zone, Tangshan City, Hebei Patentee after: Yanhua Yongle (Leting) Biological Technology Co. Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Patentee before: Beijing Yanhua Yongle Biotechnology Co., Ltd. |
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