CN103651581A - Bactericidal composition - Google Patents

Abstract

The invention belongs to the technical field of pesticides and relates to a bactericidal composition. The bactericidal composition is characterized in that the active ingredients comprise Jiean-peptide and validamycin. The composition can be prepared into suspending agents and water dispersible granules, is used for preventing and treating wheat sharp eyespot and is obvious in bactericidal effect and environment-friendly.

Description

A kind of bactericidal composition

Technical field the invention belongs to technical field of pesticide, contains specifically the bactericidal composition with synergistic effect of Jiean-peptide and jinggangmeisu.

Background technology

Jiean-peptide (jiean-peptide) derives from the metabolite of bacillus, and the pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium are all had to stronger inhibition growth and control efficiency.Field test results shows: to tomato and Botrytis cinerea, and capsicum damping off, cucumber, wheat powdery mildew etc., control efficiency is remarkable, reaches more than 85%, is a kind of novel biological bactericide that has research and development value and application prospect, has good market prospects.

Jinggangmeisu (Validamycin) is a kind of antibiotic of actinomycetes generation, has stronger interior absorption, easily by somatic cells, is absorbed and conduction rapidly within it, disturbs and inhibition somatic cells g and D.Be mainly used in rice sheath blight disease, also can be used for the control of the crop pests such as rice green smut, corn northern and southern leaf blight and vegetables and cotton, beans.Large-scale field is used result fully to show the feature of the environmental protection type agricultural chemical of its " preventive effect high, without poisoning, pollution-free " for many years, welcome by users at home and abroad.

Wheat sharp eyespot has become China wheat district regular incidence evil.After wheat is infected by the withered bacterium of line, at each growing stage, there is the symptoms such as rotten bud, the rotten stem of withered, the colored stalk of sick seedling, withered strain dead ears.Jiean-peptide and jinggangmeisu have good preventive effect to wheat sharp eyespot, have good synergistic effect after both are composite.

Summary of the invention

The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the bactericidal composition of Jiean-peptide and composite jinggangmycin.

Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make formulation of the present invention, comprise suspending agent and water dispersible granules.

In above-mentioned composite bactericide, the proportion of Jiean-peptide and jinggangmeisu is 1:60-60:1.

Jiean-peptide and validamycin compound germicide have following characteristics: 1. active ingredient is biogenic, low toxic and environment-friendly; 2. both are composite has a synergistic effect; 3. both are without cross resistance; 4. sterilizing ability is strong; 5. formulation environmental protection.

Embodiment

For determining Jiean-peptide and two kinds of composite synergistic effects of active ingredient of jinggangmeisu, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:

Beak angle load bacterium (Ceratobasidium cornigerum Rogers) belongs to Basidiomycotina fungi, is the main pathogenic fungi that causes wheat sharp eyespot, and Jiean-peptide and jinggangmeisu are fine to wheat sharp eyespot preventive effect, and we survey object by life and are decided to be this bacterium.

The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the mycelial growth of Etiology of Sharp Eyespot of Wheat bacterium.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of jinggangmeisu of the former medicine of 70% Jiean-peptide and 75% be take acetone as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Bacterium cake with card punch (Φ 5mm) the picking Etiology of Sharp Eyespot of Wheat bacterium of sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen ₅₀, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).

It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.

Indoor biometrics test data sees the following form:

Table 1 Jiean-peptide, jinggangmeisu and the measurement result of different proportioning to wheat sharp eyespot co-toxicity coefficient thereof

Analyze above-mentioned data, can obviously find out when Jiean-peptide and jinggangmeisu are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of Etiology of Sharp Eyespot of Wheat bacterium is had to the synergistic effect of different proportion, wherein Jiean-peptide and jinggangmeisu are being that proportioning four is the most remarkable to the synergy between proportioning six between 1:10-1:1, the composite desirable medicament that becomes control wheat sharp eyespot of visible Jiean-peptide and jinggangmeisu.

By indoor biometrics, test and determine Jiean-peptide and two kinds of active ingredients of jinggangmeisu are composite can good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 30.5% Jiean-peptide jinggangmeisu water dispersible granules

Example two, 6.1% Jiean-peptide jinggangmeisu suspending agent

Example three, 41% Jiean-peptide jinggangmeisu suspending agent

Example four, 3% Jiean-peptide jinggangmeisu water dispersible granules

Example five, 16.5% Jiean-peptide jinggangmeisu suspending agent

Example six, 18% Jiean-peptide jinggangmeisu suspending agent

Example seven, 5% Jiean-peptide jinggangmeisu suspending agent

Example eight, 9% Jiean-peptide jinggangmeisu suspending agent

Example nine, 31% Jiean-peptide jinggangmeisu suspending agent

Example ten, 12% Jiean-peptide jinggangmeisu suspending agent

Example 11,36% Jiean-peptide jinggangmeisu suspending agent

Select above example to prevent and treat the field trial of wheat sharp eyespot, determine the effect of formula control wheat sharp eyespot; Simultaneously by the Contrast on effect with Jiean-peptide and jinggangmeisu single dose, verify the synergistic effect after composite.Concrete test method data are as follows:

18% Jiean-peptide jinggangmeisu suspending agent and other preparation are done the test of pesticide effectiveness of field control wheat sharp eyespot.Test site is Luoyang City, and the time is on April 20th, 2012, test each and process three repetitions, and community randomized arrangement, each community area is 100m ², adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.

The grade scale of disease index:

0 grade: healthy tree, blade is without symptom;

1 grade: it is vaned below 5% that symptom blade accounts for institute;

3 grades: symptom blade accounts for vaned 6%-15%;

5 grades: symptom blade accounts for vaned 16%-25%;

7 grades: symptom blade accounts for vaned 26%-50%;

9 grades: it is vaned more than 50% that symptom blade accounts for institute.

Table 318% Jiean-peptide jinggangmeisu suspending agent and the preventive effect of different medicament to wheat sharp eyespot

From above-mentioned data, can find out, Jiean-peptide and composite jinggangmycin preparation after medicine 7 days preventive effects all over 72%, and Jiean-peptide single dose 7 days preventive effects after medicine are 51.55%, the preventive effect of jinggangmeisu single dose is 50%, compare Jiean-peptide and jinggangmeisu single dose, the preventive effect that has utmost point significant difference between complex preparation and single dose, visible two active ingredients are composite good synergistic effect.And after medicine 21 days, all complex preparations have all surpassed 80% to the preventive effect of wheat sharp eyespot, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of wheat sharp eyespot.After two compositions are composite, can improve drug effect, extend the lasting period, there is good economic benefit.

Claims (3)

1. a bactericidal composition, is characterized in that: active component is comprised of Jiean-peptide and jinggangmeisu, and the weight ratio of described Jiean-peptide and jinggangmeisu is 1:60～60:1.

2. bactericidal composition according to claim 1, is characterized in that: by active ingredient and auxiliary material, make suspending agent, water dispersible granules.

3. according to claim 1 or bactericidal composition claimed in claim 2, it is characterized in that: for preventing and treating wheat sharp eyespot.