CN103651397A - Compound bactericide - Google Patents
Compound bactericide Download PDFInfo
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- CN103651397A CN103651397A CN201310701475.9A CN201310701475A CN103651397A CN 103651397 A CN103651397 A CN 103651397A CN 201310701475 A CN201310701475 A CN 201310701475A CN 103651397 A CN103651397 A CN 103651397A
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- jiean
- peptide
- tebuconazole
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- wheat
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Abstract
The invention belongs to the technical field of pesticides, and relates to a compound bactericide. The compound bactericide is characterized in that active ingredients comprise Jiean-peptide and penconazole. Composition can be prepared into a suspension agent and water dispersible granules. The bactericidal spectrum is wide, the synergistic effect is remarkable, and the compound bactericide is environment-friendly.
Description
Technical field
The invention belongs to technical field of pesticide, contain specifically the composite bactericide with synergistic effect of Jiean-peptide and Tebuconazole.
Background technology
Jiean-peptide (jiean-peptide) derives from the metabolite of bacillus, and the pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium are all had to stronger inhibition growth and control efficiency.Field test results shows: to tomato and Botrytis cinerea, and capsicum damping off, cucumber, wheat powdery mildew etc., control efficiency is remarkable, reaches more than 85%, is a kind of novel biological bactericide that has research and development value and application prospect, has good market prospects.
A kind of efficient, wide spectrum of Tebuconazole (Tebuconazole), interior absorption triazole type sterilization pesticide, have protection, treat, root out three large functions, and fungicidal spectrum is wide, the lasting period is long.This composition is the same with all triazole bactericidal agents, the biosynthesis of the ergosterol that Tebuconazole can Antifungi.Tebuconazole is mainly used in preventing and treating the multiple fungal disease on the crops such as wheat, paddy rice, peanut, vegetables, banana, apple, pears and corn and Chinese sorghum, and it obtains registration extensive use in 60 various crop of more than 50 countries in the whole world.
Wheat sharp eyespot has become China wheat district regular incidence evil.After wheat is infected by the withered bacterium of line, at each growing stage, there is the symptoms such as rotten bud, the rotten stem of withered, the colored stalk of sick seedling, withered strain dead ears.Jiean-peptide and Tebuconazole have good preventive effect to wheat sharp eyespot, have good synergistic effect after two kinds of compositions are composite.
Summary of the invention
The object of this invention is to provide a kind of composite bactericide with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the composite bactericide of Jiean-peptide and Tebuconazole.
Utilize above-mentioned composite bactericide, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make formulation of the present invention, comprise suspending agent and water dispersible granules.
In above-mentioned composite bactericide, the weight ratio of Jiean-peptide and Tebuconazole is 1:60-60:1.
Above-mentioned composite bactericide has following characteristics: 1. active ingredient is biogenic, low toxic and environment-friendly; 2. both are composite has a synergistic effect; 3. both are without cross resistance; 4. sterilizing ability is strong; 5. formulation environmental protection.
Embodiment
For determining Jiean-peptide and two kinds of composite synergistic effects of active ingredient of Tebuconazole, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:
Beak angle load bacterium (Ceratobasidium cornigerum Rogers) belongs to Basidiomycotina fungi, is the main pathogenic fungi that causes wheat sharp eyespot, and Jiean-peptide and Tebuconazole are fine to wheat sharp eyespot preventive effect, and we survey object by life and are decided to be this bacterium.
The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the mycelial growth of Etiology of Sharp Eyespot of Wheat bacterium.On the basis of preliminary experiment, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the Original Tebuconazole of the former medicine of 70% Jiean-peptide and 95% be take acetone as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterile water; Bacterium cake with card punch (Φ 5mm) the picking Etiology of Sharp Eyespot of Wheat bacterium of sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivation 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC of each medicament to pathogen
50, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
It is synergistic effect that co-toxicity coefficient CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.
Table 1 Jiean-peptide, Tebuconazole and the measurement result of different proportioning to wheat sharp eyespot co-toxicity coefficient thereof
Analyze above-mentioned data, can obviously find out when Jiean-peptide and Tebuconazole are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of Etiology of Sharp Eyespot of Wheat bacterium is had to the synergistic effect of different proportion, wherein Jiean-peptide and Tebuconazole are being that proportioning five is the most remarkable to the synergy between proportioning seven between 1:5-5:1, the composite desirable medicament that becomes control wheat sharp eyespot of visible Jiean-peptide and Tebuconazole.
By indoor biometrics, test and determine Jiean-peptide and two kinds of active ingredients of Tebuconazole are composite can good synergistic effect, below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 61% Jiean-peptide tebuconazole water dispersible granule
Example two, 42% Jiean-peptide tebuconazole suspension concentrates
Example three, 33% Jiean-peptide tebuconazole suspension concentrates
Example four, 24% Jiean-peptide tebuconazole water dispersible granule
Example five, 10% Jiean-peptide tebuconazole suspension concentrates
Example six, 30% Jiean-peptide tebuconazole suspension concentrates
Example seven, 22% Jiean-peptide tebuconazole suspension concentrates
Example eight, 16.8% Jiean-peptide tebuconazole suspension concentrates
Example nine, 30.5% Jiean-peptide tebuconazole suspension concentrates
Example ten, 15% Jiean-peptide tebuconazole suspension concentrates
Example 11,20% Jiean-peptide tebuconazole suspension concentrates
Select above example to prevent and treat the field trial of wheat sharp eyespot, determine the effect of formula control wheat sharp eyespot; Simultaneously by the Contrast on effect with Jiean-peptide and Tebuconazole single dose, verify the synergistic effect after composite.Concrete test method data are as follows:
20% Jiean-peptide tebuconazole suspension concentrates and other preparation are done the test of pesticide effectiveness of field control wheat sharp eyespot.Test site is Zhumadian prefecture, Henan province city, and the time is on April 16th, 2012, test each and process three repetitions, and community randomized arrangement, each community area is 100m
2, adopting back-pack electric compression sprayer, dispenser was sunny calm the same day, and the planting habit of all communities is all identical with local planting habit, records the dispenser same day and the synoptic data of a week thereafter, specific as follows:
Table 2 duration of test meteorological data
Investigation method is: fixed 2 points of each community diagonal, and every some straight ahead is determined 25 clumps, and after dispenser, fixed point investigation in the 7th day, 21 days, investigates each community disease and refers to, calculates preventive effect, and carries out the new multipole difference analysis of Deng Kenshi.
The grade scale of disease index:
0 grade: healthy tree, blade is without symptom
1 grade: it is vaned below 5% that symptom blade accounts for institute
3 grades: symptom blade accounts for vaned 6%-15%
5 grades: symptom blade accounts for vaned 16%-25%
7 grades: symptom blade accounts for vaned 26%-50%
9 grades: it is vaned more than 50% that symptom blade accounts for institute
Table 315% Jiean-peptide tebuconazole suspension concentrates and the preventive effect of different medicament to wheat sharp eyespot
From above-mentioned data, can find out, Jiean-peptide and Tebuconazole complex preparation after medicine 7 days preventive effects all over 72%, and Jiean-peptide single dose 7 days preventive effects after medicine are 52.75%, the preventive effect of Tebuconazole single dose is 51.43%, compare Jiean-peptide and Tebuconazole single dose, the preventive effect that has utmost point significant difference between complex preparation and single dose, visible two active ingredients are composite good synergistic effect.And after medicine 21 days, all complex preparations have all surpassed 80% to the preventive effect of wheat sharp eyespot, and the lasting period of composite rear preparation extends, more lasting to the control efficiency of wheat sharp eyespot.After two compositions are composite, can improve drug effect, extend the lasting period, there is good economic benefit.
Claims (3)
1. a composite bactericide, is characterized in that: active component is comprised of Jiean-peptide and Tebuconazole, and the weight ratio of described Jiean-peptide and Tebuconazole is 1:60～60:1.
2. composite bactericide according to claim 1, is characterized in that: by active ingredient and auxiliary material, make suspending agent, water dispersible granules.
3. according to claim 1 or composite bactericide claimed in claim 2, it is characterized in that: for preventing and treating wheat sharp eyespot.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701475.9A CN103651397B (en) | 2013-12-18 | 2013-12-18 | Compound bactericide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701475.9A CN103651397B (en) | 2013-12-18 | 2013-12-18 | Compound bactericide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103651397A true CN103651397A (en) | 2014-03-26 |
| CN103651397B CN103651397B (en) | 2015-01-07 |
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|---|---|---|---|
| CN201310701475.9A Active CN103651397B (en) | 2013-12-18 | 2013-12-18 | Compound bactericide |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1339287U (en) * | ||||
| CN1698428A (en) * | 2005-05-13 | 2005-11-23 | 胡玉莲 | High-tension winding ceramic disc of electronic insects killing lamp |
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| WO2010046927A2 (en) * | 2008-10-16 | 2010-04-29 | Deepak Pranjivandas Shah | Use of synergistic composition |
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2013
- 2013-12-18 CN CN201310701475.9A patent/CN103651397B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1339287U (en) * | ||||
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| CN1698428A (en) * | 2005-05-13 | 2005-11-23 | 胡玉莲 | High-tension winding ceramic disc of electronic insects killing lamp |
| WO2010046927A2 (en) * | 2008-10-16 | 2010-04-29 | Deepak Pranjivandas Shah | Use of synergistic composition |
Non-Patent Citations (1)
| Title |
|---|
| 钟娟 等: "一种新型抗菌肽—捷安肽素的研究", 《中国生物工程学会第四次会员代表大会暨学术讨论会论文摘要集》, 1 July 2005 (2005-07-01), pages 113 * |
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| CN103651397B (en) | 2015-01-07 |
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Inventor after: Yang Huiying Inventor after: Liu Xiaojiao Inventor after: Ma Songyue Inventor after: Shen Dongxia Inventor after: Luo Xueting Inventor after: Li Qiumei Inventor after: Xu Bo Inventor before: Yang Huiying Inventor before: Liu Xiaojiao Inventor before: Wu Yong Inventor before: Ma Songyue Inventor before: Wang Zhandi |
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Effective date of registration: 20171221 Address after: 063600 Leting County Economic Development Zone, Tangshan City, Hebei Patentee after: Yanhua Yongle (Leting) Biological Technology Co. Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Patentee before: Beijing Yanhua Yongle Biotechnology Co., Ltd. |